| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_000913 | 3,560,455:1 | +G | 100% | pseudogene (151/758 nt) | glpR ← | DNA‑binding transcriptional repressor GlpR |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 3,560,455 | 1 | . | G | 91.2% | 55.4 / ‑3.6 | 24 | pseudogene (151/758 nt) | glpR | DNA‑binding transcriptional repressor GlpR |
| Reads supporting (aligned to +/- strand): ref base . (1/0); major base G (13/8); minor base A (1/1); total (15/9) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.28e-01 | |||||||||||
| Rejected as polymorphism: E-value score below prediction cutoff. | |||||||||||
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. | |||||||||||
AAACAGCGTCGAGCCATTGGGGATTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGGC‑CAGGAT‑CAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGA > NC_000913/3560319‑3560588 | aaaCAGCGTCGAGCCATTGGGGATTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCGTGCGATCGTGCCACGGCGTCTTACCCGAACTGGAAGGCCGGGCAGCACCGCCCTGGTGGcacaggc > 2:264088/1‑137 (MQ=255) cgggtgcGAGCCATTGGGGATTTGCTCCGCCACGTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGGAT‑Ca < 2:240146/137‑1 (MQ=255) ttGGGGATTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGGAT‑CAGGTTTTGCTCCGc < 1:118486/143‑1 (MQ=255) gATTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCGTGCGATCGTGCCACGGCGTGTTAACCGGACTGGAAGGCAGCGCCGCAACGCGATGATGGCGCAGGAT‑CAGCATAGGCTCCGCCAGcg > 2:457472/1‑142 (MQ=255) ccGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGGAT‑CAGGTTTTGCTCCGCCAGCTCATTGAgg > 1:233966/1‑143 (MQ=255) cggctcgttGCGGGCGGTGCGCTCTTGTGCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTATCCGTACTGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGGAT‑CAGGTTTTGCTCCGCCAGCTCATTGAGGt < 2:540683/136‑1 (MQ=255) cGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGGAT‑CAGGTTTTGCTCCGCCAGCTCATTGAGGt < 1:641293/143‑1 (MQ=255) tttGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGGAT‑CCGGCATTGCACCTCCAGCGCATTGAGGTCgcggc > 2:321208/1‑143 (MQ=255) gggCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGGAT‑CAGGTTTTGCTCCGCCAGCTCATCGAGGTCGCGGCGAATa > 2:137885/1‑143 (MQ=255) gggCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGGAT‑CAGGTTTTGCTCCGCCAGCCCATCGAGGTCGCGGCGactc > 2:667270/1‑140 (MQ=255) cgcTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGGAT‑CAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCgg > 2:542330/1‑143 (MQ=255) tttttCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGGAT‑CAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAGTCGTCTGCGGGCTGa > 1:5814/1‑143 (MQ=255) cGGTCCGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGGAT‑CAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGaaa < 1:368022/143‑1 (MQ=255) gTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGGAT‑CAGGTTTTGCTCCGCCAGCCCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGCCGGAGAGATg > 2:381870/1‑143 (MQ=255) cTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGGAT‑CAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGGAATGCTCTACCAGct > 1:500884/1‑143 (MQ=255) cGTGCAACGGCGTGTTGACCGAGCGGGAAGGCGGTGCCGCACCGCCATGATGGCGCAGGCT‑CAGGTTTTGCGCCGCCAGCTCATTGAGGTCGCGGCGAATCGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTa < 2:400055/143‑1 (MQ=255) gTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCAACGCCAAGACAAT‑GTAAATGCTTGTTATGGACTAGTTACTCATTGAGGTCGCGGCGAATAGACTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTAc > 1:676176/1‑143 (MQ=255) gCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGGCACAGGAT‑CAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATaa > 1:679610/1‑143 (MQ=255) cGTGTTACCCGACCTGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGGAT‑CAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAAc < 1:34525/143‑1 (MQ=255) gCAGCGCCGCACCGCCATGATGGCGCAGGAT‑AAGGTGTTGCTCGGCCAGCTCATTGAGGTAGCGGAGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACTAGATGTTCGGTACTGAGCTAACCATGATGTGTAAACAGCTCg > 2:372937/1‑143 (MQ=255) cgccggACCGCCATGATGGCGCAGGAT‑CAGGTCTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCCGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACCTAACCCTGCTGTTTAACCAGTTCGataa < 1:264088/143‑1 (MQ=255) cgccgcACCGCCATGCTGGCGCAGGAT‑CAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAAGAGTCTGCGGGCTGACGGCGGAATGCTCTACCCGCTCTTCGGTACTGACATAACCCTGCTGTCTAGCCAGTTCGacaa > 1:593701/1‑143 (MQ=255) gccgcACCGCCATGATGGCGCAGGAT‑CAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGGTTAACCAGTCCGataat > 2:175464/1‑143 (MQ=255) cgcACCGCCATGATGGCGCAGGAT‑CAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGCTAATAc > 1:132364/1‑143 (MQ=255) cATGGTGGCACAGGAT‑CAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGa < 1:417688/143‑1 (MQ=255) | AAACAGCGTCGAGCCATTGGGGATTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGGC‑CAGGAT‑CAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGA > NC_000913/3560319‑3560588 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |