Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
JC JC	NC_000913	1,979,486	IS5 (+) +4 bp	100%	intergenic (‑271/‑264)	insA‑5 ← / → uspC	IS1 protein InsA/universal stress protein C

New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	NC_000913	1979486 =	0 (0.000)	41 (1.170)	34/264	NT	100%	intergenic (‑271/‑267)	insA‑5/uspC	IS1 protein InsA/universal stress protein C
	?	NC_000913	2101749 =	NA (NA)					noncoding (1195/1195 nt)	IS5	repeat region
*	?	NC_000913	= 1979489	0 (0.000)	23 (0.660)	23/264	NT	100%	intergenic (‑274/‑264)	insA‑5/uspC	IS1 protein InsA/universal stress protein C
	?	NC_000913	= 2290113	NA (NA)					noncoding (1/1195 nt)	IS5	repeat region

AATCACTCCCGGTGATCATATAATTTCAAATGTAATTATAAGAAAATAGTGTACTGATTCTCCGCATTAAACAAGTGGTTAATATATGTAAAACATTTCATTAACATTAAGTTGATTGTTGCCTTTCTTTGTATTTAATTAG‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  <  NC_000913/1979627‑1979486 ‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑GGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTTCTCCCGTAAATGCCTTGAATCAGCCTATTTAGACCGTTTCTTCGCCATTTAAGGCGTTATCCCCA  >  NC_000913/2101749‑2101890                                                                                                                                                                                                                                                                                              AATCACTCCTGGTGATCATATAATTTCAAATGTAATGATAAGAAAATAGTGTACTGATTCTCCGCATTAAACAAGTGGTTAATATATGTAAAACATTTCATTAACATTAAGTTGATTGTTGCCTTTCTTTGTATTTAATTAGG                                                                                                                                               <  2:146330/143‑1       TCCCGGTGATCATATAATTTCAAATGTAATTATAAGAAAATAGTGTACTGATTCTCCGCATTAAACAAGTGGTTAATATATGTAAAACATTTCATTAACATTAAGTTGATTGTTGCCTTTCTTTGTATTTAATTAGGGAAGGT                                                                                                                                         >  2:222878/1‑143         CCGTTGATCATATAATTTCAAATGTAATTATAAGAAACTAGTGTACTGATTCTCCGCATTAAACAAGTGGTTAATATATGTAAAACATTTCATTAACATTAAGTTGATTGTTGCCTTTCTTTCTATTTAATTAGGGAAGGTGC                                                                                                                                       <  2:595491/143‑1          CGGTGATCATATAATTTCAAATGTAATTATAAGAAAATAGTGTACTGATTCTCCGCATTAAACAAGTGGTTAATATATGTAAAACATTTCATTAACATTAAGTTGATTGTTGCCTTTCTTTGTATTTAATTAGGGAAGGTGCG                                                                                                                                      >  1:135088/1‑143                         TTCAAATGTAATTATAAGAAAATAGTGTACTGATTCTCCGCATTAAACAAGTGGTTAATATATGTAAAACATTTCATTAACATTAAGTTGATTGTTGCCTTTCTTTGTATTTAATTAGGGAAGGTGCGAATAAGCGGGcgaaa                                                                                                                       >  2:170064/1‑138                            AAATGTAATTATAAGAAAATAGTGTACTGATTCTCCGCATTAAACAAGTGGTTAATATATGTAAAACATTTCATTAACATTAAGTTGATTGTTGCCTTTCTTTGTGTTTAATTAGGGAAGGTGCGAATAAGCGGGGCAATTCT                                                                                                                    >  1:546660/1‑143                            AAATGTAATTATAAGAAAATAGTGTACTGATTCTCCGCATTAAACAAGTGGTTAATATATGTAAAACATTTCATTAACATTAAGTTGATTGTTGCCTTTCTTTGTATTTAATTAGGGAAGGTGCGAATAAGCGGGGAAATTCT                                                                                                                    <  1:547661/143‑1                                GTAATTATAAGAAAATAGTGTACTGATTCTCCGCATTAAACAAGTGGTTAATATATGTAAAACATTTCATTAACATTAAGTTGATTGTTGCCTTTCTTTGTATTTAATTAGGGAAGGTGCGAATAAGCGGGCAAATTCTTCTC                                                                                                                >  2:519405/1‑143                                GTAACTATAAGAAAATAGTGTACTGATTCTCGGCATTAAACAAGTGGTTAATATATGTAAAACATTTCATTAACATTAAGTTGATTGTTGCCTTTCTTTGTATTTAATTAGGGAAGGTGCGAATAAGCGGGGAAATTCTTCTC                                                                                                                <  2:243081/143‑1                                  AATTATAAGAAAATAGTGTACTGATTCTCCGCATTAAACAAGTGGTTAATATATGTAAAACATTTCATTAGCATTAAGTTGATTGTTGCCTTTCTTTGTATTTAATTAGGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGG                                                                                                              <  2:599821/143‑1                                    TTATAAGAAAATAGTGTACTGATTCTCCGCATTAAACAAGTGGTTAATATATGTAAAACATATCATTAAAAATAAAGTGATTGTTGCCATTCTTTGTACTTAATTAGGGAAGGAGCGAATAAGCGGGGAAATTCTTCTCGGCa                                                                                                            >  2:44576/1‑142                                          GAAAATAGTGTACTGATTCTCCGCATTAAACAAGTGGTTAATATATGTAAAACATTTCATTAACATTAAGTTGATTGTTGCCTTTCTTTGTATTTAATTAGGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCA                                                                                                      >  1:77534/1‑143                                                   GTACTGATTCTCCGCATTACACAAGTGGTTAATATATGTACAACATTTCATTAACATTAAGTTGATTGTTGCCTTTCTTTGTATCTAATTAGGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCA                                                                                             <  1:553648/143‑1                                                               CGCATTAAACAAGTGGTTAATATATGTAAAACATTTCATTAACATTAAGTTGATTGTTGCCTTTCTTTGTATTTAATTAGGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTT                                                                                 >  2:470792/1‑143                                                                GCATTAAACAAGTGGTTAATATATGTAAAACATTTGATTAACATTAAGTTGATTGTTGCCTTTCGTTGTATTTTATGAGGGAAGGTGCGAATAAGCGGGGAAAGTCTTCTCGGCTGATTAAGTCATTTCATTTCTTCATGTTT                                                                                >  2:517262/1‑143                                                                  ATTAATCAAGTGGTTAATATATGTAAATCATTTCATTAACATTAAGTTGATTGTTGCCTTTCTTTGTATTTAATTAGGGAAGGTGCTAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGA                                                                              <  2:16108/143‑1                                                                   TTAAACAAGTGGTTAATATATGTAAAACATTTCATTAACATTAAGTTGATTGTTGCCTTTCTTTGTATTTAATTAGGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAG                                                                             <  1:303346/143‑1                                                                         AAGTGGTTAATATATGTAAAACATTTCATTAACATTAAGTTGATTGTTGCCTTTCTTTGTATTTAATTAGGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATT                                                                       >  1:503432/1‑143                                                                            TGGTTAATATATGTAAAACATTTCATTAACATTAAGTTGATTGTTGCCTTTCTTTGTATTTAATTAGGGAAGGTGCGAATACGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTT                                                                    <  1:608418/143‑1                                                                            TGGTTAATATATGTAAAACATTTCATTAACATTAAGTTGATTGTTGCCTTTCTTTGTATTTAATTAGGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGCCTCAGTCACTTCATTTCTTCATGTTTGAGCCGATTTTT                                                                    <  1:610135/143‑1                                                                            TGGTTAATATATGTAAAACATTTCATTAACATTAAGTTGATTGTTGCCTTTCTTTGTATTTAATTAGGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTT                                                                    >  2:11092/1‑143                                                                                   TATATGTAAAACATTTCATTAACATTAAGTTGATTGTTGCCTTTCTTTGTATTTAATTAGGGAAGGTGCGAATACGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTTCTCTCG                                                             >  2:189719/1‑143                                                                                   TATATGTAAAACATTTCATTAACATTAAGTTGATTGTTGCCTTTCTTTGTATTTAATTAGGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTTCTCCCG                                                             >  1:9823/1‑143                                                                                    ATATGTAAAACATTTCATTAACATTAAGTTGATTGTTGCCTTTCTTTGTATTTAATTAGGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTTCTCCCGT                                                            <  1:75241/143‑1                                                                                     tcTGTAAAACATGTTATTAACATTAAGTTGATTGTTGCCTTTCTTGGTATTTAATTAGGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTTCTCCCGTA                                                           <  2:322332/141‑1                                                                                         TAAAACATTTCATTAACATTAAGTTGATTGTTGCCTTTCTTTGTATTTAATTAGGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTTCTCCCGTAAATG                                                       >  1:270731/1‑143                                                                                         TAAAACATTTCATTAACATTAAGTTGATTGTTGCCTTTCTTTGTATTTAATTAGGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTAGAGCCGATGTTTTCTACCGTAAATG                                                       >  2:221827/1‑143                                                                                                TTTCATTAACATTAAGTTGATTGTTGCCTTTCTTTGTATTTAATTAGGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTTCTCCCGTAAATGCCTTGAA                                                >  1:647544/1‑143                                                                                                 TTCATTAACATTAAGTTGATTGTTGCCTTTCTTTGTATTTAATTAGGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCTTTTCTTCATGTTTGAGCCGATTTTTTCTCCCGTAAATGCCTTGAAT                                               >  1:566651/1‑143                                                                                                 TTCATTAACATTAAGTTGATTGTTGCCTTTCTTTGTATTTAATTAGGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATATCTTCCCGTTCAATCCGATTTTTTCTCCCGTAAATGCCTTGAAT                                               >  1:639055/1‑143                                                                                                   CATTAACATTAAGTTGATTGTTGCCTTTCTTTGTATTTAATTAGGGAAGGTGCGAATAAGCGGTGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTTCTCCCGTAAATGCCTTGAATCA                                             <  2:363815/143‑1                                                                                                          gttgcGTTGATGGTTGTGTTTCTTTGTGTTTGATTAGGGAAGGTGCGAAGAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTGAGGTTTGAGCTGATTTTTTCTCCCGTAAATGCCTTGAATCAGCCTATT                                      <  2:670797/138‑1                                                                                                          ATTAAGTTGATTGTTGCCTTTCTTTGTATTTAATTAGGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGTATCATTCATTTCATTTCTTCATGTTTGAGCCGATTTTTTCTCCCGTAAATGCCTTGAATCAGCCTATT                                      <  2:327910/143‑1                                                                                                            TAAGTTGTTTGTTGCCTTTCTTTGTATTTAATTAGGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTTCTCCCGTAAATGCCTTGAATCAGCCTATTTA                                    <  2:495278/143‑1                                                                                                                 TGATTGTTGCCTTTCTTTGTATTTAATTAGGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTTCTCCCGTAAATGCCTTGAATCAGCCTATTTAGACCG                               <  1:222878/143‑1                                                                                                                          CCTTTCTTTGTATTTAATTAGGGAAGGTGCGAATAAGCGGCGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTGGAGCCGATTTTTTCTCCCGTAAATGCCTTGAATCAGCCTATTTAGACCGTTTCTTCGC                      <  2:336864/143‑1                                                                                                                                  TGTATTTAATTAGGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTTCTCCCGTAAATGCCTTGAATCAGCCTATTTAGACCGTTTCTTCGCCATTTAAG              <  1:166415/143‑1                                                                                                                                  TGTATTTAATTAGGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTTCTCCCGTAAATGCCTTGAATCAGCCTATTTAGACCGTTTCTTCGCCATTTAAG              <  2:77534/143‑1                                                                                                                                    TATTTAATTAGGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTTCTCCCGTAAATGCCTTGAATCAGCCTATTTAGACCGTTTCTTCGCCATTTAAGGC            <  1:74420/143‑1                                                                                                                                      TTTAATTAGGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTTCTCCCGTATGTGCCTTGAATCAGCCTATTTAGACTGTGTCTTAGCCATCTAATGCGT          >  1:320426/1‑143                                                                                                                                       TTAATTAGGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTTCTCCCGTAAATGCCTTGAATCAGCCTATTTAGACCGTTTCTTCGCCATTTCAGGCGct         >  2:502742/1‑141                                                                                                                                         AATTAGGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTGATTTCTTCATGTTTGAGCCGATTTTTTCTCCCGTATATGCCTTGAATCAGCCTCTTTAGACCGTTCCTTCGACAATTTAAGCGTTga       >  2:259067/1‑141                                                                                                                                           TTAGGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGCTTTTTTCTCCCGTAACTGCCTTGAATCAGCCTACTTAGCCCGCTTCTCCGCCCTTCAACGCGTcaccc     >  2:472520/1‑138                                                                                                                                           TTAGGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTTCTCCCGTAAATGCCTTGAATCAGCCTATTTAGACCGTTTCTTCGCCATTTAAGGCGTTATCC     >  1:537465/1‑143                                                                                                                                              GGGAAGGTGCTAATAGGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTTCTCCCGTAAATGCCTTGAATCAGCCTATTTAGACCGTTTCTTCGCCATTTAAGGCGTTATCCCCA  <  2:632125/143‑1                                                                                                                                              GGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTTCTCCCGTAAATGCCTTGAATCAGCCTATTTAGGCCGTTTCTTCGCCATTTAAGGCGTTATCCCCA  >  1:10383/1‑143                                                                                                                                              GGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTTCTCCCGTAAATGCCTTGAATCAGCCTATTTAGACCGTTTCTTCGCCATTTAAGGCGTTATCCCCA  <  2:379805/143‑1                                                                                                                                                                                                                                                                                              AATCACTCCCGGTGATCATATAATTTCAAATGTAATTATAAGAAAATAGTGTACTGATTCTCCGCATTAAACAAGTGGTTAATATATGTAAAACATTTCATTAACATTAAGTTGATTGTTGCCTTTCTTTGTATTTAATTAG‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  <  NC_000913/1979627‑1979486 ‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑GGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTTCTCCCGTAAATGCCTTGAATCAGCCTATTTAGACCGTTTCTTCGCCATTTAAGGCGTTATCCCCA  >  NC_000913/2101749‑2101890

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.