| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_000913 | 123,050 | A→T | 100% | I12F (ATC→TTC) | aceE → | pyruvate dehydrogenase E1 component |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 123,050 | 0 | A | T | 99.8% | 69.7 / ‑7.5 | 27 | I12F (ATC→TTC) | aceE | pyruvate dehydrogenase E1 component |
| Reads supporting (aligned to +/- strand): ref base A (1/0); new base T (11/15); total (12/15) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.44e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
| Rejected as polymorphism: E-value score below prediction cutoff. | |||||||||||
| Rejected as polymorphism: Frequency below/above cutoff threshold. | |||||||||||
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. | |||||||||||
AGCGCGGCAACTAAACGTAGAACCTGTCTTATTGAGCTTTCCGGCGAGAGTTCAATGGGACAGGTTCCAGAAAACTCAACGTTATTAGATAGATAAGGAATAACCCATGTCAGAACGTTTCCCAAATGACGTGGATCCGATCGAAACTCGCGACTGGCTCCAGGCGATCGAATCGGTCATCCGTGAAGAAGGTGTTGAGCGTGCTCAGTATCTGATCGACCAACTGCTTGCTGAAGCCCGCAAAGGCGGTGTAAACGTAGCCGCAGGCACAGGTATCA > NC_000913/122911‑123188 | aGCGCGGCAACTAAACGTAGAACCTGTCTTATTGAGCTTTCCGGCGAGAGTTCAATGGGACAGGTTCCAGACAACTCAACGTTATTAGATAGATAAGGAATAACCCATGTCAGAACGTTTCCCAGATGACGTGGATCCGTTCg > 1:177340/1‑143 (MQ=255) gTCGTATTGAGCTTTCTGGCGAGAGTTCAATGGGACAGGTTCCAGAAAACTCAACGTTATTAGATAGATAAGGAATAACCCATGTCAGAACGTTTCCCAAATGACGTGGATCCGTTCGAAACTCGCGACTGGCTCCAGGCGAt < 2:59433/143‑1 (MQ=255) tCTTATGGAGCTTTCCGGCGAGAGTTCAACGGGACAGGTTCCAGAAAACTCAACGTTATTAGATAGATACGGAATAACCCCTGTCAGAACGTTTCCCAAATGACGTGGATCCGTTCGAAACTCGCGACTGGCTCCAGGCGATc < 1:248593/143‑1 (MQ=255) tATTGAGCTTTCCGGCGAGAGTTCAATGGGACAGGTTCCAGAAAACTCAACGTTATTAGATAGATAAGGAATAACCCATGTCAGAACGTTTCCCAAATGACGTGGATCCGTTCGAAACTCGCGACTGGCTCCAGGCGATCGaa < 2:274366/143‑1 (MQ=255) ttGAGCGTTCCGGCGAGAGTTCAATGGGACAGGTTCCAGAAAACTCAACGTTATTAGATAGATAAGGAATAACCCATGTCAGAACGTTTCCCAAATGACGTGGATCCGTTCGAAACTCGCGACTGGCTCCAGGCGATCGAATc < 2:559551/143‑1 (MQ=255) cTTTCCGGCGAGAGTTCAATGGGACAGGTTCCAGAAAACTCAACGTTATTAGATAGATAAGGAATAACCCATGTCAGAACGTTTCCCAAATGACGTGGATCCGTTCGAAACGCGCGACTGGCTCCAGGCGATCGAATCGGTCa > 2:242916/1‑143 (MQ=255) ttCCGGCGAGAGTTCAATGGGACAGGTTCCAGAAAACTCAACGTTATTAGATAGATAAGGAATAACCCATGTCAGAACGTTTCCCAAATGACGTGGATCCGTTCGAAACTCGCGACTGGCTCCAGGCGATCGAATCGGTCATc > 1:474125/1‑143 (MQ=255) ggCGAGAGTTCAATGGGACAGGTTCCAGAAAACTCAACGTTATTAGATAGATAAGGAATAACCCATGTCAGAACGTTTCCCAAATGACGTGGATCCGTTCGAAACTCGCGACTGGCTCCAGGCGATCGAATCGGTCATCCGTg > 1:85292/1‑143 (MQ=255) ggCGAGAGTTCAATGGGACAGGTTCCAGAAAACTCAACGTTATTAGATAGATAAGGAATAACCCATGTCAGAACGTTTCCCAAATGACGTGGAAAACACCGGTTCTCGCGACAGCCCCCAGGCGATCGACTCGGTCATCCGTg > 2:402344/1‑143 (MQ=255) agTTCAATGGGACAGGTTCCAGAAAACTCAACGTTATTAGATAGATAAGGAATAACCCATGTCAGAGCGTTTCCCAAATGACGTGGATCCGTTCGAAACTCGCGACTGGCTCCAGGCGCTCGGATCGGTCATCCGTggcgagg > 1:811/1‑137 (MQ=255) ttCAATGGGACAGGTTCCAGAAAACTCAACGTTATTAGATAGATAAGGAATAACCCATGTCAGAACGTTTCCCAAATGACGTGGATCCGTTCGAAACTCGCGACTGGCTCCAGGCGATCGACTCGGTCATCCGTGAAGAAGgt > 1:130255/1‑143 (MQ=255) gACAGGTTCCAGAAAACTCAACGTTATTAGATAGATAAGGAATAACCCATGTCAGAACGTTTCCCAACTGCCGTGGATCCGTTCGAAACTCGCGACTGGCTCCAGGCGATCGAATCGGTCATCCGTGAAGCAGGTGTTGAGCg > 1:556182/1‑143 (MQ=255) ttCCAGAATACTTATCGTTATTAGATAGATAAGGAATAACCCATGTCAGAACGTTTCCCAAATGACGTGGATCCGTTCGAAACTCGCGACTGGCTCCAGGCGATCGAATCGGTCATCCGTGAAGAAGGTGTTGAGCGTGCTCa < 2:908/143‑1 (MQ=255) aaCTCAACGTTATTAGATAGATAAGGAATAACCCATGTCAGAACGTTTCCCAAATGACGTGGATCCGTTCGAAACTCGCGACTGGCTCCAGGCGATCGAATCGGTCATCCGTGAAGAAGGTGTTGAGCGTGCTCAGTATCTGa < 1:70488/143‑1 (MQ=255) tCAACGTTATTAGATAGATAAGGAATAACCCATGTCAGAACGTTTCCCAAATGACGTGGATCCGTTCGAAACTCGCGACTGGCTCCAGGCGATCGAATCGGTCATCCGTGAAGAAGGTGTTGAGCGTGCTCAGTATCTGATCg > 2:536238/1‑143 (MQ=255) ttaGAGAGAGAAGGAATAACCCATGTCAGTACGTTTCCCAAATGACTTGGATCCGTTCGAAACTCGCGACTGGCTCCAGGCGATCGAATCGGTCATCCGTGAAGAAGGTGTTGAGCGTGCTCAGTATCTGATCGACCAACtgc < 2:552796/143‑1 (MQ=255) tagataGGGAATAGCCCAGGCGAGAGCGTGTCCCAAATGACGTGGATCCGTTCGAAACTCGCGACTGGCTCCAGGCGATCGAATCGGTCATCCGTGAAGAAGGTGTTGAGCGTGCTCAGTATCTGATCGACCAACtgcttgct < 2:542138/143‑1 (MQ=255) agataAGGAATAACCCATGTCAGAACGTTTCCCAAATGACGTGGATCCGTTCGAAACTCGCGACTGGTTCCAGGCGATCGAATCGGTCATCCGTGAAGAAGGTGTTGAGCGTGCTCAGTATCTGATCGACCAACTGCTTGCTg < 2:637122/143‑1 (MQ=255) aaGGAATAACCCATGTCAGAACGTTTCCCAAATGACGTGGATCCGTTCGAAACTCGCGACTGGCTCCAGGCGATCGAATCGGGCATCCGTGAAGAAGGTGTTGAGCGTGCTCAGTATCTGATCGACCAACTGCTTGCTGAAGc > 1:495146/1‑143 (MQ=255) aaGGAACAACCGATGCAAGAACGTCTCCCAAATGACGTGAATCCGTTCGAAACTCGCGACTGGCTCCAGGCGTTCGAATCGGTCATCCGTGAACAAGGTGTTGAGCGTGCTCAGTATCTGATCGACCAACTGCTTGCTGAAGc < 2:385247/143‑1 (MQ=255) aGGAATAACCCATGTCAGAACGTTTCCCAAATGACGTGGATCCGTTCGAAACTCGCGACTGGCTCCAGGCGATCGAATCGGTCATCCGTGAAGAAGGTGTTGAGCGTGCTCAGTATCTGATCGACCAACTGCTTGCTGAAGcc > 1:315579/1‑143 (MQ=255) gtGTCATAATATTTCGCAATTGCCGTTGTTCTGTTCTAATCTCGCTACTGTCTCAAGGCGATCGAATCGGTCATCCGTGAAGAATGTGTTGAGCGTGCTCAGTATCTGATCGACCAACTGCTTGCTGAAGCCCGCAAAGGCgg < 2:126016/142‑1 (MQ=255) tgccAGAACGCTTCCCAAGTGACGTGGATCCGTTCGAAACTCGCGACTGTCTCCAGGCGATCGAATCGGTCATCCGTGAAGAAGGTGTTGAGCGTGCTCAGTATCTGATCGACCAACTGCTTGCTGAAGCCCGCAAAGGCGgt < 1:566003/140‑1 (MQ=255) gTCAGAACGTTGCCCAAGTGACGTGGATCCGTTCGAAACTCGCGACTGGCTCCAGGCGATCGAATCGGTCATCCGTGAAGAAGGGGTTGAGCGTGCTCAGTATCTGATCGACCAACTGCTTGCTGAAGCCCGCAAAGGCGgtg < 1:355453/143‑1 (MQ=255) gTCACAACGTCTCCCAAATGCCGTGGATCCGTTCGAAACTCGCGACTGGCTCCAGGCGATCGAATCGGTCATCCGTGAAGAAGGTGTTGAGCGTGCTCAGTATCTGATCGACCCACTGCTTGCTGAAGCCCGCAAAGGCGgtg < 1:359638/143‑1 (MQ=255) aaaTGACGTGGATCCGTTCGAAACTCGCGACTGGCTCCAGGCGATCGAATCGGTCATCCGTGAAGAAGGTGTTGAGCGTGCTCAGTATCTGATCGACCAACTGCTTGCTGAAGCCCGCAAAGGCGGTGTAAACGTAGCCGCAg < 1:322725/143‑1 (MQ=255) tCCGTTCGAAACTCGCGACTGGCTCCAGGCGATCGACTCGGTCATCCGTGAAGAAGGCGTTGAGCGTGCTCAGTATCTGATCGACCAACTGCTTGCTGAAGCCCGCAAAGGCGGTGTAAACGTAGCCGCAGGCACAGGTATCa > 1:331611/1‑143 (MQ=255) | AGCGCGGCAACTAAACGTAGAACCTGTCTTATTGAGCTTTCCGGCGAGAGTTCAATGGGACAGGTTCCAGAAAACTCAACGTTATTAGATAGATAAGGAATAACCCATGTCAGAACGTTTCCCAAATGACGTGGATCCGATCGAAACTCGCGACTGGCTCCAGGCGATCGAATCGGTCATCCGTGAAGAAGGTGTTGAGCGTGCTCAGTATCTGATCGACCAACTGCTTGCTGAAGCCCGCAAAGGCGGTGTAAACGTAGCCGCAGGCACAGGTATCA > NC_000913/122911‑123188 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |