| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_000913 | 1,557,343 | C→T | 17.6% | T232T (ACG→ACA) | ddpF ← | putative D,D‑dipeptide ABC transporter ATP‑binding subunit DdpF |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 1,557,343 | 0 | C | T | 17.6% | 48.1 / 3.2 | 28 | T232T (ACG→ACA) | ddpF | putative D,D‑dipeptide ABC transporter ATP‑binding subunit DdpF |
| Reads supporting (aligned to +/- strand): ref base C (7/16); new base T (3/2); total (10/18) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 3.15e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.53e-01 | |||||||||||
AACGTTCGTAGAAAAAACAGCCTTGCGGCAACGTGCGGTTTCCCGGCAGATCCGTTTTACGTAATGCCCATTCTTCCTCCAGCGGTTTATCAATGGCGGGGAGGGAATCCAGCAATAATCGGGTGTATGGATGTGCAGGTGCCGTCAGCACCTGCTGCGCGTCGCCCAGTTCTACAATCTGCCCGAGATACATCACCGCCACCCGATCGCTCATATGACGTATCACCGAGACATTGTGTGAAATCAGCACATAGGTCAGCCCGTGATTTTCCTGTAGCGTTACCA > NC_000913/1557201‑1557485 | aaCGTTCGTAGAAAAAACAGCCTTGCGGCAACGTGCGGTTTCCCGGCATATCCGTTTTACGTAATGCCCATTCTTCCTCCAGCGGTTTATCAATGGCGGGGAGGGAATCCAGCAATAATCGGGTGTATGGATGTGCAGGTGcc < 1:631498/143‑1 (MQ=255) tcgtcgTAAAACGAGACTTGCGGCAAGGGGCGGTTGCCCGGCAGATCCGTCTTTCGTGATGCCCATTCTTCCGCCAGCGGTTTATGAATGGCGGGGAGGGAATCCAGCAATAAGCGGGTGTATGGATGTGCAGGTGCCGTCAg < 2:592703/143‑1 (MQ=255) tCGTAGAAAAACCGGCCTTGCGGCAACGTGCGGTTTCCCGGCAGATCCGTTTTACGTAATGTCCATTCTTCCTCCAGCGGTTTATCAATGGCGGGGAGGGAATCCAGCAATAATCGGGTGTATGGATGTGCAGGTGCCGTCAg < 2:504305/143‑1 (MQ=255) gAAAAAACAGCCTTGCGGCAACGTGCGGTTTCCCGGCAGATCCGTTTTACGTAATGCCCATTCTTCCTCCAGCGGTTTATCAATGGCGGGGAGGGAATCCAGCAATAATCGGGTGTATGGATGTGCAGGTGCCGTCAGCACct < 1:545896/143‑1 (MQ=255) aaagaaCAGCCTTGCGGCAACGCGCGGTTTCCCGGCAGATCCGTTTTACGTAATGCCCATTCTTCCTCCAGCGGTTTTTCAATGGCGGGGAGGGAATCCAGCAATAATCGGGTGTATGGATGTGCAGGTGCTGACGGCACctg < 2:357460/143‑1 (MQ=255) aaaatgcAGCCTTGCGGGAACGTGCGGTTTCCCGGCAGATCCGTTTTACGTAATGCCCATTCTTCCTCCAGCGGTTTATCAATGGCGGGGAGGGAATCCAGCAATAATCGGGTGTATGGATGTGCAGGTGCTGACGGCACctg < 2:658959/137‑1 (MQ=255) accAGCCTTGCGGCAACGTGCGGTTCCCCGGCCGCTCCGTTTTACGTAATGCCCATTCTTCCTCCAGCGGTTTATCAATGGCGGGGCGGGAATCCAGCAATAATCGCGTGTATGGATGTGCAGGTGCCGTCAGCACCtgctgc < 1:511243/141‑1 (MQ=255) cAGCCTTGCGGCAACGTGCGGTTTCCCGGCAGATCCGTTTTACGTAATGCCCATTCTTCCTCCAGCGGTTTATCAATGGCGGGGAGGGAATCCAGCAATAATCGGGTGTATGGATGTGCAGGTGCCGTCAGCACCTGCTgcgc < 1:477031/143‑1 (MQ=255) ttGCGGCAACGTGCGGTTTCCCGGCAGATCCGTTTTACGTAATGCCCATTCTTCCTCCAGCGGTTTATCAATGGCGGGGAGGCAATCCAGCAATAATCGGGGGTATGGATGTGCAGGTGCCGTCAGCACCTGCTGCGCGTCGc > 2:110855/1‑143 (MQ=255) cAACGTGCGGTTTCCCGGCAGATCCGTTTTACGTAATGCACATGATTATATCGTTGAAGAATCACTGGCAGCCATTGTATCCAGCAATAATCGGGTGTATGGATGTGCAGGTGCCGTCAGCACCTGCTGCGCGTCGCCCAGtt < 2:660190/143‑1 (MQ=255) gcgCGGTTTCCCGGCAGATCCGTTTTACGTAATGCCCATGCTTCCTCCAGCGGTTTATCAATGGCGGGGAGGGAATCCAGCAATAATCGGGTGTATGGATGTGCAGGTGCCGTCAGCACCTGCTGCGCGTCGCCCAGTTCTAc < 1:27311/141‑1 (MQ=255) gTTTCCCGGCAGATCCGTGTTACGTAATGCCCATTCTTCCTCCAGCGGATTATCAATGGCGCGGAGGGACTCCAGCAATCAGCGGGTGGATGGGTGCGCAGGGGCCGCCAGCACCAGACGCGCGGCGCCCGGTTCTCCAAGCt > 2:261924/1‑143 (MQ=255) cagCAGATCCGTTTTACGGAATGCCCATTCTTCCTCCAGCGGTTTATCAATGGCGGGAAGGGAATCCAGCAATAATCGGGGGTATGGATGTGCGGGTGCCGTCGGCACCTGCGGCACGTCGCCCAGTTGTACAGTCTGCCCga > 2:555170/3‑143 (MQ=255) gtccATTCTCCTTCCAGCGTTCTGTCAATGTCGGGGACGGTATCCAGCAATAATCGGGTGTATGGATGTGCAGGTGCCGTCAGCACCTGCTGCGCGTCGCCCAGTTCTACAATCTGCCCTAGATACATCACCGCCACCCGATc < 2:382860/141‑1 (MQ=255) gCCCATTCTTCCTCCAGCGGTTTATCGATGGCGGGGAGGGAATCCAGCAATAATCGGGTGTATGTATGTGCAGGTGCCGTCAGCACCTGCTGCGCGTCGCCCAGTTCTACAATCTGCCCGAGATACATCACCGCCACCCGATc < 2:546726/143‑1 (MQ=255) ccATTCTTCCTCCATCGGTTTATCAATGGCGGGGAGGGAATCCCGCAATAATCGGGTGTATGGCTGTGCAGGTGCTGTCAGCAGCTGCTGCGCGTCGGCCAGTTCTACAATCTGCACGAGGCACATCGCGGCCGCCCgccagc > 2:434199/1‑138 (MQ=255) ccATTCTGCCTCCAACAGTTGGTCGGTGGCGGGGAGGGAATCCAGCAATAAGCGGGTGTATGGATGTGCAGGTGCCGTCAGCACCTGCTGCGCGTCGCCCAGTTCTACAATCTGCCCGAGATACATCACCGCCACCCGATCGc < 1:120647/143‑1 (MQ=255) aTTCTTCCTCCAGCGGTTTATCAATGGCGGGGAGGGAATCCAGCAATAATCGGGTGAATGGATGTGCAGCTGCCGTCAGCACCTGCCGGGAGTCGCCCAGTTCCACAATCTCCCCGGGCTGCGGCACCGCCACCCGAGCGCtg > 2:77072/1‑142 (MQ=255) ttcttcGTCCAGCGGGTTATCAATGGCGGGGAGGGAATCCAGCAATAATCGGGTGTATGGATGTGCAGGTGCCGTCAGCACCTGCTGCGCGTCGCCCAGTTCTACAATCTGCCCGAGATACATCACCGCCACCCGATCGCTCa < 2:306082/143‑1 (MQ=255) gTTTATCAATGGCGGGGAGGGAATCCAGCAATAATCGGGTGTATGGATGCAAAGTCCTTGCCTGTGGTTTTTGCGGGTCGCCCAGTTCTACAATCTGCCCGAGATACGTCACCGCCACCCGATCGCTCATATGACGTATcccc > 1:126586/1‑143 (MQ=255) tttATCAATGGCGGGGCAGCAATCGAGCAATAATAGGGTGTATGGATGCGCAGGTGCTGTCAGCACGAGCGGCGCGTCGCCCAGTACTACAATCTGCCCGAGATACAACACCGGCGCGCGGTGGCGCATAGGCCATATCaacc > 2:468010/1‑140 (MQ=255) aaTGGCCGAGAGGGAATCCAGCAATAATCGGGTGTATGGATGTGCAGGTGCCGTCAGCACCGGCTGCGAGTCGCCCAGTTCTACAATCGGCCCGAGAAACAACACCGCCACCCGATCGCAGCTATGACGTAGCACCGAGAAAt > 2:116789/1‑143 (MQ=255) gagggaATCCAGCAATAATCGGGTGTATGGATGTGCAGGTGCCGTCAGCACCTGCTGCGCGTCGCCCAGTTCTACAATCTGCCCGAGATACAGCACCGCCACCCGATCGTTCATAGGACGGATCACGGAGAAACTGGGCGaaa > 2:616960/1‑143 (MQ=255) aaTCCAGCAATAATCGTGTGTATGGATGTGCAGGTGCCGTCAGCACCTGCTGCGCGTCGCCCAGTTCTACAATCTGCCCGAGATACATCACCGCCACCCGATCGCTCATATGACGTATCACCGAGACATTGTGTGAAATCAGc < 1:626972/143‑1 (MQ=255) taatCGGGTGTATGGATGTGCAGGTGCCGTCAGCACCTGCTGCGCGTCGCCCAGTTCTACAATCTGCCCGAGATACATCACCGCCACCCGATCGCTCATATGACGTATCACCGAGACATTGTGTGAAATCAGCACATAGGTCa < 1:35776/143‑1 (MQ=255) gggTGTATGGATGTGCAGGTGCCGTCAGCACCTGCTGCGCGTCGCCCAGTTCTACACTCTGCCCGAGATACATCACCGCCACCCGATCGCTCATATGACGTATCACCGAGACATTGTGTGAAATCAGCACATAGGTCAGCCCg < 2:126586/143‑1 (MQ=255) gaTGGAGGTGCAGGTGCCGTCAGCACCTGCTGCGCGTCGCCCAGTTCTACAATCTGCCCGAGATACATCACCGCCACCCGATCGCTCATATGACGTATCACCGAGACATTGTGTGAAATCAGCACATAGGTCAGCCCGTGAtt < 2:650817/142‑1 (MQ=255) ggTGCCGTCAGCACCTGCTGCGCGTCGCCCAGTTCTACAATCTGCCCGAGATACATCACCGCCACCCAATCGCTCATATGAGGTATCAGCGAGACATTGCGAGAAAACAGCCCACAGGGCCGCACGTGAGTGTCCTggcacgg > 2:491799/1‑137 (MQ=255) gCCGTCAGCACCTGCTGCGCGTCGCCCAGTTCTACAATCTGCGCGAGATACATCACCGCCACCCGATCGCTCATATGACGTATCACCGAGACATTGTGTGAAATCAGCACATAGGTCAGCCCGTGATTTTCCTGTAGCGTTAc < 2:156116/143‑1 (MQ=255) cGTCAGCACCTGCTGCGCGTCGCCCAGTTCTACAATCTGCCCGAGATACATCACCGCCACCCGATCGCTCATATGACGTATCACCGAGACATTGTGTGAAATCAGCACATAGGTCAGCCCGTGATTTTCCTGTAGCGTTACCa < 1:110855/143‑1 (MQ=255) | AACGTTCGTAGAAAAAACAGCCTTGCGGCAACGTGCGGTTTCCCGGCAGATCCGTTTTACGTAATGCCCATTCTTCCTCCAGCGGTTTATCAATGGCGGGGAGGGAATCCAGCAATAATCGGGTGTATGGATGTGCAGGTGCCGTCAGCACCTGCTGCGCGTCGCCCAGTTCTACAATCTGCCCGAGATACATCACCGCCACCCGATCGCTCATATGACGTATCACCGAGACATTGTGTGAAATCAGCACATAGGTCAGCCCGTGATTTTCCTGTAGCGTTACCA > NC_000913/1557201‑1557485 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |