| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_000913 | 1,559,325 | C→A | 21.6% | A196S (GCG→TCG) | ddpC ← | putative D,D‑dipeptide ABC transporter membrane subunit DdpC |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 1,559,325 | 0 | C | A | 21.6% | 37.1 / 10.3 | 28 | A196S (GCG→TCG) | ddpC | putative D,D‑dipeptide ABC transporter membrane subunit DdpC |
| Reads supporting (aligned to +/- strand): ref base C (7/15); new base A (4/2); total (11/17) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.74e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.83e-01 | |||||||||||
GTTGAGCACCCAGGCCAATAAATCCCAACGTGGCGGCCATTAAAATCGCGCTACCGATATCCAGCGATGCCTGCACGATCAGCGGCGGTAGGGAGTTACGTAAAATATGCCAGTTGATCAGATGCCAACGAGACGCGCCAAAGGTTTTCGCCGCCTGAACATAGGTATACTGGCGTACAACTAATGCTTGCCCCCGCGCCAGGCGCACATAAAAGGGAATTCGCACAATAGCAATAGCCAGCATGGCGTTAAACAAACTCGGCCCGAGAGCGGCAGC > NC_000913/1559190‑1559466 | gtttcgCACCCAGGCCAATAAGTGCTGTTGTGGCGGCCATTAGAACCGCGCTAGCGATGCCCAGCGATGCCTGCACGATCAGCGGCGGTAGGGAGTTACGTAAAATAGGCCAGTTGATCAGATGCCAACGAGACGCGCCAAAg < 2:650754/138‑1 (MQ=255) ttgAGCACCCACGCCACTAACTCCCGACGTCTCGGCCATTAAAATCGCGCTACCGATATCCAGCGATTCCTGCACGATCAGCGGCGGTAGGGAGTTACGTAAAATATGCCAGTTGATCAGATGCCAACGAGACGCGCCAAAgg < 2:164861/143‑1 (MQ=255) gAGCCCCCAGGCCCATAGATCCCCACGTGGCGGCCATTAAAATCGCGCTACCGATATCCAGCGATGCCTGCACGATCAGCGGCGGTAGGGAGTTACGTAAAATATGCCAGTTGATCAGATGCCAACGAGACGCGCCAAAGGtt < 2:154749/143‑1 (MQ=255) cACCCAGGCCAATAAATCCCAACGTGGCGGCCATTAAAATCGCGCTACCGATATCCAGCGATGGCTGCACGATCAGCGGCGGTAGGGAGTTACGTAAAATATGCCAGTTGATCAGATGCCAACGAGACGCGCCAAAGGTTTTc < 1:427279/143‑1 (MQ=255) aCCCAGGCCAATAAATCCCAACGTGGCGGCCATTAAAATCGCGCTACCGATATCCAGCGATGCCTGCACGATCAGCGGCGGTAGGGAGTTACGTAAAATATGCCAGTTGATCAGATGCCAACGAGACGAGCCAAAGGTTTTcg > 1:592416/1‑143 (MQ=255) aCCCAGGCCAATAAATCCCAACGTGGCGGCCATTAAAATCGCGCTACCGATATCCAGCGATGCCTGCACGATCAGCGGCGGTAGGGAGTTACGTAAAATATGCCAGTTGATCAGATGCCAACGAGACGAGCCAAAGGTTTTcg > 1:594108/1‑143 (MQ=255) cAGGCCAATAAATCCCAACGTGGCGGCCATTAAAATCGCGCTACCGATATCCCGCGCTGCCTGCACGGTCCGCGGCGGTAGGGAGTTACGTCAAATATGCCAGTTGATCAGACGCCACCGCGACGCGCCCACGGCTTccgccg > 2:625654/1‑143 (MQ=255) aaTAAATCCCAACGTGGCGGCCATTAAAATCGCGCTACCGATATCCAGCGATGCCTGCACGATCAGCGGCGGTAGGGAGTTACGTAAAATATGCCAGTTGATCAGATGCCAACGAGACGCGCCGAAGGCTTTCGCCCCCGGaa > 2:220783/1‑143 (MQ=255) aaTAAATCCCAACGTGGCGGCCATTAAAATCGCGCTACCGATATCCAGCGATGCCTGCACGATCAGCGGCGGTAGGGAGTTACGTAAAATATGCCAGTTGATCAGATGCCAACGAGACGAGCCAAAGGTTTTCGCCGCCTGaa > 1:222765/1‑143 (MQ=255) tCCCAACGTGGCGGCCATTAAAATCGCGCTACCGGTATCCCGCGATGCCTGCACGAGCAGCGGCGGTAGGGAGTTACGTAAAATATGCCAGTTGATCAGATGCCAACGAGACGCGCCAAAGGTTTTCGCCGCCTGAACATAgg < 2:404346/143‑1 (MQ=255) gctaTTCAAATCGCGCTACCGATCTCCACCGATGCCTGCACGATCAGCGGCGGTAGGGAGTTACGTAAAATACGCCAGTTGATCAGATGCCAACGAGACGCGCCAAAGGTTTTCGCCGCCTGAACATAGGTATACTGGCGTAc < 1:457847/140‑1 (MQ=255) cATTAAAATCGCGCTACCGATCTCCAGCGATGCCTGCACGAGCAGCGGCGGTAGGGAGTTACGTAAAATAGGCCAGTTGATCAGATGCCAACGAGACGCGCCAAAGGTTTTCGCCGCCTGAACATAGGTATACTGGCGTACaa < 2:231237/143‑1 (MQ=255) aaaaTGGCGCTCCAGTGATCCAGCGATGCCTGCACGATCAGCGGCGGTAGGAAGTTACGTAACATATGCCAGTTGATCAGATGCCAACGAGACGCGCCAAAGGTTTTCGCCGCCTGAACATAGGTATACTGGCGTACAACTaa < 2:294445/143‑1 (MQ=255) aTCGCGCTACCGATATCCAGCGATGCCTGCACGATCAGCGGCGGTAGGGAGCTCCGTAAAATATGCCAGTTGATCAGATGCCAACGAGACGCGCCAAAGGTTTTCGCCGCCTGGACATAGGTATACTGGCGTACAACTAATGc > 1:651417/1‑143 (MQ=255) tCGCGCTACCGATATCCAGCGATGCCTGCACGATCAGCGGCGGTAGGGAGTTACGTAACATATGCCAGTTGATCAGATGCCAACGAGACGCGCCCAAGGTCTTCGGCGCCCGCACATAGGTACACTGGCGCCCAACCAATGcc > 2:570368/1‑142 (MQ=255) tGCACGATCAGCGGCGGTAGGGCGTTACGTAAAATATGCCAGTTGATCAGCTGCCAACGAGACGCGCCAAAGGTTTTCGCCGCCTGAAAAAAGGGATCCTGGCGTACCACTAATGCTTCACCCCGCGCCCGGCACACATcaaa > 2:445092/1‑143 (MQ=255) cGATCAGCGGCGGTAGGGAGTTACGTAAAATATGCCAGTTGATCAGATGCCAACGAGACGAGCCAAAGGTTTTCGCCGCCTGAACATAGGTATACTGGCGTACAACTAATGCTTGCCCCCGCGCCAGGCGCACATAAAAGGGa < 1:658138/143‑1 (MQ=255) gcggcgGTAGGCAGTTACGTAAAATATGCCAGTTGATCAGAGGCCAACGGTACGCGCCAAAGGGTTTCGCCGCCTGAACATAGGTATACTGGCGTACAACTAATGCTTGCCCCCGCGCCAGGCGCACATAAAAGGGAATTCGc < 2:77444/143‑1 (MQ=255) cggTAGGGAGTTACGTAAAATATGCCAGTTGATCAGCTGCCAACGAGACGCGCCAAAGGTTTTCGCCGCCTGCACATAGGTATACTGGCGTACGACTAATGCTTGCCCCCGCGCCAGGCGCACATAAAAGGGAATTCGCAcaa > 1:176434/1‑143 (MQ=255) ggAGTTACGTAAAATATGCGAGTTGATCAGATGCCAACGAGACGAGCCAAAGGTTTTCGCCGCCTGAACATAGGTATACTGGCGTACAACTAATGCTTGCCCCCGCGCCAGGCGCACATAAAAGGGAATTCGCAcaatagcaa < 1:505082/143‑1 (MQ=255) aCGTAAAATATGCCAGTGGATCAGATGCCAACGAGACGCGCCAAAGGTTTTCGCCGCCTGAACATAGGTATACTGGCGTACAACTAATGCTTGCCCCCGCGCCAGGCGCACATAAAAGGGAATTCGCACAATAGCAATAGCCa < 2:171675/143‑1 (MQ=255) cGTAAAATATGCCAGTTGATCAGATGCCAACGAGACGCGCCAAAGGTTTTCGCCGCCTGAACATAGGTATACTGGCGTACAACTAATGCTTGCCCCCGCGCCAGGCGCACATAAAAGGGAATTCGCACAATAGCAATAGCCAg < 1:567384/143‑1 (MQ=255) tGCCAGTTGATCAGATGCCAACGAGACGCGCCAAAGGACTTCGCCGCCTGAACATAGCTATACCGGCGTACAACTAATGCTCGCCCCCGCGCCAGGCGGACAGGCAAGAGAACACGCACCACCGCCAGAGCCAGCACggggca > 2:64465/1‑139 (MQ=255) gCCTGTGGATCAGAGGCCGACGAGACGCGCCAAAGGTTTTCGCCGCCTGAACATAGGTATACTGGCGTACAACTAATGCTTGCCCCCGCGCCAGGCGCACATAAAAGGGAATTCGCACAATAGCAATAGCCAGCATGGCGTTa < 2:71254/143‑1 (MQ=255) gCCAGTTGATCAGATGCCAACGAGACGAGCCAAAGGTTTTCGCCGCCTGAACATAGGTATACTGGCGTACAACTAAAGAGCATTCTTCGGCCAGGCGCACATAAAAGGGAATTCGCACAATAGCAATCGCCAGCATGGCGGTa > 1:494796/1‑143 (MQ=255) gATCAGAGGCCAACGAGACGCGCCAAAGGTTTTCGCCGCCTGAACATAGGTATACTGGCGTACAACTAATGCTTGCCCCCGCGCCAGGCGCACATAAAAGGGAATTCGCACAATAGCAATAGCCAGCATGGCGTTaaacaaac < 2:513136/143‑1 (MQ=255) tccgATGCCACCGAGACGCGCCAAAGGTGTTCGCCGCCTGAACATAGGTATACTGGCGTACAACTAATGCTTGCCCCCGCGCCAGGCGCACATAAAAGGGAATTCGCACAATAGCAATAGCCAGCATGGCGTTAAACAAACTc < 1:635372/140‑1 (MQ=255) cgagtccAAGGATACGCGCCAAAGTCTGTCGCCGCCTGAACATAGGTATACTGGCGTACAACTAATGCTTGCCCCCGCGCCAGGCGCACATAAAAGGGAATTCGCACAATAGCAATAGCCAGCATGGCGTTAAACAAACTCgg < 1:668954/138‑1 (MQ=255) gcgcCAAAGGTTTTCGCCGCCTGAACATAGGTATACTGGCGTACAACTAATGCTTGCCCCCGCGCCAGGCGCACATAAAAGGGAATTCGCACAATAGCAATCGCCAGCATGGCGTTACACAAACTCGGCCCGAGACCGCCAGc > 2:124802/1‑143 (MQ=255) | GTTGAGCACCCAGGCCAATAAATCCCAACGTGGCGGCCATTAAAATCGCGCTACCGATATCCAGCGATGCCTGCACGATCAGCGGCGGTAGGGAGTTACGTAAAATATGCCAGTTGATCAGATGCCAACGAGACGCGCCAAAGGTTTTCGCCGCCTGAACATAGGTATACTGGCGTACAACTAATGCTTGCCCCCGCGCCAGGCGCACATAAAAGGGAATTCGCACAATAGCAATAGCCAGCATGGCGTTAAACAAACTCGGCCCGAGAGCGGCAGC > NC_000913/1559190‑1559466 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |