Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,934,850 C→A 82.3% E489* (GAG→TAG)  zwf ← NADP(+)‑dependent glucose‑6‑phosphate dehydrogenase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,934,8500CA82.3% 53.2 / 2.3 28E489* (GAG→TAG) zwfNADP(+)‑dependent glucose‑6‑phosphate dehydrogenase
Reads supporting (aligned to +/- strand):  ref base C (2/3);  new base A (8/14);  total (10/18)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

AGTCAGTGTAATAAAAAAAGCCTCGTGGGTGAATCCGCACGAGGCCTGAAAGTGTAAAAATTGTTCTACAATCTGCGCAAGATCATGTTACCGGTAAAATAACCATAAAGGATAAGCGCAGATATTACTCAAACTCATTCCAGGAACGACCATCACGGGTAATCATCGCCACCGAGGCAACGGGTCCCCAGGTTCCGGCCTGATACGGTTTCGGCGCATCATTGTCCATCGCCCACGCCTCAGTAATGGAGTCTACCCATTTCCAGGCTTCTTC  >  NC_000913/1934715‑1934988
                                                                                                                                       |                                                                                                                                          
agtcagtGTAATAAAAAAAGCCTCGTGGGTGAATCCGCACGAGGCCTGAAAGTGTAAAAATTGTTCTACAAGCTGCGCAAGATCATGTTACCGGGAAACTAACCATAAAGGATAAGAGCAGATATTACTCAAACTCATTCCAg                                                                                                                                     >  2:328289/1‑143 (MQ=255)
  gcagcGTAATAAAACAAGCCTCGTGGGTGAAGCCGCACGAGGCCTGAACGTGTAAAGACTGTTCTACAATCTGCGCAAGATCATGTTACCGGTAATATAACCATAAAGGATAAGCGCAGATATTACTCAAACTAATTCCAGGa                                                                                                                                   <  2:195553/142‑1 (MQ=255)
   cagtGTAATAAAAAAAGCCTCGTGGGTGAATCCGCACGAGGCCTGAAAGTGTAAAAATTGTTCTACAATCTGCGCAAGATCATGTTACCGGTAAAATAACCATAAAGGATAAGCGCAGATATTACTCAAACTAATTCCAGGaa                                                                                                                                  <  1:376087/143‑1 (MQ=255)
    agtGTAATAAAAAGAGCCTCGTGGGTGAATCCGCACGAGGACTGAAAGTGTAAAAATTGTTCTACAATCTGCGCAAGATCATGTTACCGGTAAAATAACCATAAAGGATAAGCGCAGATATTACTCAAACTAATTCCAGGAAc                                                                                                                                 <  1:344804/143‑1 (MQ=255)
        taatatAAAAAGCCTCGTGGGTGAATCCGCACGAGGCCTGAAAGTGTAAAAATTGTTCTACAATCTGCGCAAGATCATGTTACCGGTAAAATAACCATAAAGGATAAGCGCAGATATTACTCAAACTAATTCCAGGAACGAcc                                                                                                                             <  2:559293/143‑1 (MQ=255)
                                 tCCGCACGAGGCCTGAAAGTGTAAAAATTGTTCTACAATCTGCGCCCGATCATGTTACCGGTAAAATAACCATAAAGGATAAGCGCAGATATTACTCAAACTAATTCCAGGAACGACCATCACGGGTACTCATCGCCACCggg                                                                                                    >  2:618553/1‑141 (MQ=255)
                                 cccGCACGAGGCCTGAAAGTGTAAAAATTGTTCTACAATCTGCGCAAGATCATGTTACCGGTAAAATAACCATAAAGGATAAGCGCAGATATTACTCAAACTCATTCCAGGAACGACCATCACGGGTAATCATCGCCACCGAg                                                                                                    >  1:614626/2‑143 (MQ=255)
                                        gAGGCCTGAAAGTGTCAAAATTGTTCTACAATCTGCGCAAGATCATGTTACCGGTAAAATAACCATAAAGGATAAGCGCAGATATTACGCAAACTAATTCCAGGAACGACCATCACGGGTAATCATCGCCACCGAGGCAACgg                                                                                             <  2:3057/143‑1 (MQ=255)
                                            ccTGAAAGTGTAAAAATTGTTCTACAATCTGCGCAAGATCATGTTACCGGTAAAATAACCATAAAGGATAAGCGCAGATATTACTCAAACTAATTCCAGGAACGACCATCACGGGTAATCATCGCCACCGAGGCAACGGGTcc                                                                                         >  1:139126/1‑143 (MQ=255)
                                              tGAAAGTGTAAAAATTGTTCTACAATCTGCGCAAGATCATGTTACCGGTAAAATAACCATAAAGGATAAGCGCAGATATTACTCAAACTAATTCCAGGAACGACCATCACGGGTAATCATCGCCACCGAGGCAACGGGTcccc                                                                                       >  1:475687/1‑143 (MQ=255)
                                               gAAGGTGTGAAACTTGTTGTACAATCCGCGCAAGATCATGTTAGCGGTACAATAACCATAGAGGATAATCGCCGATATTACTCAAACTAATTCCAGGAACGACCATCTCGGGTAATCATCGCCACCGAGGCAACGGGTCCCCa                                                                                      <  2:32211/143‑1 (MQ=255)
                                                          aaTTGTTCTACAATCTGCGCAAGATCATGTTACCGGTAAAATAACCATAAAGGATAAGCGCAGATATTACTCAAACTAATTCCAGGAACGACCATCACGGGTAATCAACGCCACCGCGGCAACGGGCCCCCAGTTTCCGGcga                                                                           >  2:260756/1‑141 (MQ=255)
                                                          aaTTGTTCTACAATCTGCCCAAGATCACGTTACCGGTAAAATAACCATAAAGGATAAGCGCAGATATTACTCAAACTAATTCCAGGAACGACCATCACGGGTAATCATCGCCACCGAGGCAACGGGTCCCCAGGTTCCGGCCt                                                                           <  1:498383/143‑1 (MQ=255)
                                                                    cAATCTGCGCAAGATCATGTTACCGGTAAAATAACCATAAAGGATAAGCGCAGATATTACTCAAACTCATTCCAGGAACGACCATCACGGGTAATCATCGCCACCGAGGCAACGGGTCCCCAGGTTCCGGCCTGATACGGttt                                                                 <  2:180428/143‑1 (MQ=255)
                                                                                  tCATGTTACCGGTAAAATAACCATAAAGGATAAGCGCAGATATTACTCAAACTAATTCCAGGAACGACCATCACGGGTAATCATCGCCACCGAGGCAACGGGTCCCCAGGTTCCGGCCTGATACGGTTTCGGCGCATCATTGt                                                   >  1:168892/1‑143 (MQ=255)
                                                                                       ttACCGGTAAAATAACCATAAAGGATAAGCGCAGATATTACTCAAACTAATTCCAGGAACGACCATCACGGGTAATCATCGCCACCGAGGCAACGGGTCCCCAGGTTCCGGCCTGATACGGTTTCTGCGCATCCTCGTcaaat                                              >  1:187418/1‑139 (MQ=255)
                                                                                                  aTAACCATAAAGGATAAGCGCAGATATTACTCAAACTAATTCCAGGAACGACCATCACGGGTAATCATCGCCACCGAGGCAACGGGTCCCCAGGTTCCGGCCTGATACGGTTTCGGCACATCATTGTCCATCGCCCACGCCTc                                   <  2:83254/143‑1 (MQ=255)
                                                                                                   tAACCATAAAGGATAAGCGCAGATATTACTCAAACTAATTCCCGGCACGGCCCGCACTGTCAATCATCGCCACCGAGGCAACGGGTCCCCAGGTTCCGGCCTGATACGGTTTCGGCGCATCATTGTCCATCGCCCACGCCTCa                                  <  1:153700/143‑1 (MQ=255)
                                                                                                     aCCATAAAGGATAAGCGCAGATATTACTCAAACTAATTCCAGGAACGACCATCACGGGTAATCATCGCCACCGAGGCAACGGGTCCCCAGGTTCCGGCCTGATACGGTTTCGGCGCATCATTGTCCATCGCCCACGCCTCAGt                                <  2:524804/143‑1 (MQ=255)
                                                                                                         tAAGGGATAAGCGCAGGTATTACTCAAACGAATTCCGGGAACGACCATCACGGGTAATCATCGCCACCGAGGCAACGGGTCCCCAGGTTCCGGCCTGATACGGTTTCGGCGCATCATTGTCCATCGCCCACGCCTCAGTAATg                            <  2:245076/143‑1 (MQ=255)
                                                                                                          aaaGGATAAGCGCAGATATTACTCAAACTCATTCCAGGAACGACCATCACGGGTAATCATCGCCACCGAGGCAACGGGTCCCCAGGTTCCGGCCTGATACGGTTTCGGCGCATCATTGTCCATCGCCCACGCCTCAGTAATgg                           <  2:148188/143‑1 (MQ=255)
                                                                                                                tAAGCGCAGATATTACTCAAACTAATTCCAGGAACGACCATCACGGGTAATCATCGCCACCGAGGCAACGGGTCCCCAGGTTCCGGCCTGATACGGTTTCGGCGCATCATTGTCCATCGCCCACGCCTCAGTAATGGAGTCTa                     <  1:132875/143‑1 (MQ=255)
                                                                                                                      cAGATATTACTCAAACTAATTCCAGGAACGACCATCACGGGTAATCATCGCCACCGAGGCAACGGGTCCCCAGGTTCCGGCCTGATACGGTTTCGGCGCATCATTGTCCATCGCCCACGCCTCAGTAATGGAGTCTACCCAtt               >  1:320533/1‑143 (MQ=255)
                                                                                                                           atgaCTCAAACTAATTCCAGGAACGACCATCACGGGTAATCATCGCCACCGAGGCAACGGGTCCCCAGGTTCCGGCCTGATACGGTTTCGGCGCATCATTGTCCATCGCCCACGCCTCAGTAATGGAGTCTACCCATTTCCAg          <  2:394262/140‑1 (MQ=255)
                                                                                                                           actACTCAAACTGATTCCAGGAACGACCATCACGGGTAATCATCGCCACCGAGGCAACGGGTCCCCAGCGTCCGGCCTGATACGGTTTCGGCGCATCATTGTCCATCGCCCACGCCTCAGTAATGGAGTCTACCCATTTCCAg          <  2:526809/141‑1 (MQ=255)
                                                                                                                             tACTCAAACTAATTCCAGGAACGACCATCACGGGTAATCATCGCCACCGAGGCAACGGGTCCCCAGGTTCCGGCCTGATACGGTTTCGGCGCATCATTGTCCATCGCCCACGCCTGAGTAATGGAGTCTACCCACTCCCAGGc        >  1:387705/1‑143 (MQ=255)
                                                                                                                             tACTCAAACTAATTCCAGGAACGACCATCACGGGTAATCATCGCCACCGAGGCAACGGGTCCCCAGGTTCCGGCCTGATACGGTTTCGGCGCATCATTGTCCATCGCCCACGCCTCAGTAATGGAGTCTACCCATTTCCAGGc        <  1:432639/143‑1 (MQ=255)
                                                                                                                                   aaCTCATTGCAGGAACGGCCCTCACGGGTAATCATCGCCACCGAGGCAACGGGTCCCCAGGTTCCGGCCTGATACGGTTTCGGCGCATCATTGTCCATCGCCCACGCCTCAGTAATGGAGTCTACCCATTTCCAGGCttcttc  <  2:291740/143‑1 (MQ=255)
                                                                                                                                       |                                                                                                                                          
AGTCAGTGTAATAAAAAAAGCCTCGTGGGTGAATCCGCACGAGGCCTGAAAGTGTAAAAATTGTTCTACAATCTGCGCAAGATCATGTTACCGGTAAAATAACCATAAAGGATAAGCGCAGATATTACTCAAACTCATTCCAGGAACGACCATCACGGGTAATCATCGCCACCGAGGCAACGGGTCCCCAGGTTCCGGCCTGATACGGTTTCGGCGCATCATTGTCCATCGCCCACGCCTCAGTAATGGAGTCTACCCATTTCCAGGCTTCTTC  >  NC_000913/1934715‑1934988

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑