| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_000913 | 1,935,690 | C→T | 30.5% | V209I (GTT→ATT) | zwf ← | NADP(+)‑dependent glucose‑6‑phosphate dehydrogenase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 1,935,690 | 0 | C | T | 30.5% | 32.6 / 22.8 | 36 | V209I (GTT→ATT) | zwf | NADP(+)‑dependent glucose‑6‑phosphate dehydrogenase |
| Reads supporting (aligned to +/- strand): ref base C (10/15); new base T (4/7); total (14/22) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.62e-01 | |||||||||||
CAGGTCAGACGGCGGAGACATCGCAATCATGCAAAGAATTTGCAGCAGGTGGTTCTGGATCATGTCGCGCATCTGACCGGCTTTATCAAAATAGCCCCAGCGCCCTTCGATCCCCACTTCTTCTGCCACGGTAATCTCAACATGATCAATGGTGCGATTGTCCCAGTTATTCACAAACAGGGAGTTAGCAAAACGCAGCGCCAACAGGTTCAGCACCGTTTCTTTACCAAGATAGTGGTCGATACGGTAAACCTGGCACTCCTCGAAGTATTCGCCAACC > NC_000913/1935550‑1935829 | cAGGTCAGACGGCCGAGACAGCGCAATCATGCAAAGCAGTTGCAGCAGGCGGTTCTGGACCATGTCGCGCATCTGACCGGCTTTATCAAAATAGCCCCAGCGCCCTTCGATCCCCACTTCTTCTGCCACGGTAATCTCAatat < 1:22821/143‑1 (MQ=255) gACGGCGGAGACATCGCTATCATGCAAAGAATTTGCAGCAGGTGGTTCTGGATCATGTCGCGCATCTGACCGGCTTTATCAAAATAGCCCCAGCGCCCTTCGATCCCCACTTCTTCTGCTATGGTAATCTCAATATGATCAAt < 2:413002/143‑1 (MQ=255) gACGGCGGAGACATCGCAATCATGCAAAGAATTTGCAGCAGGTGGTTCTGGATCATGTCGCGCATCTGACCGGCTTTATCAAAATAGCCCCAGCGCCCTTCGATCCCCACTTCTTCTGCCACGGTAATCTCAACATGATCAAt < 2:58230/143‑1 (MQ=255) cgggaggAGACAGCGCAATCACGCAAAGGATTTGCAGCAGGTGGTTCTGGATCATGTCGCGCATCTGACCGGCTTTATCAAAATAGCCCCAGCGCCCTTCGATCCCCACTTCTTCTGCCACGGTAATCTCAATATGATCAATg < 1:68810/138‑1 (MQ=255) gcggAGACACCGCAATCATGCAAAGAATTTGCAGCAGGTGGTTCTGGATCATGTCGCGCATCTGTCCGGCTTTATCAAAATCGCCCCAGCGCCCTTCTACCCCCACTTCTTCTGCCACGGTAATCTCAACATGATCAATGGTg < 1:47556/143‑1 (MQ=255) cggAGACATCGCAATCATGCAAAGAATTTGCAGCAGGTGGTTCTGGATCATGTCGCGCATCTGACCGGCTTTATCAAAATAGCCCCAGCGCCCTTCGATCCCCACTTCTTCTGCCACGGTAATCTCAACATGATCAATGGTGc > 1:405660/1‑143 (MQ=255) aTCGCAATCATGCAAAGAATTTGCAGCAGGTGGTTCTGGATCATGTCGCGCATCTGACCGGCTTTATCAAAATAGCCCCAGCGCCCTTCGATCCCCACTTCTTCTGCCACGGTAATCTCAACATGATTAGTGTTGCGCTCGTc > 2:317621/1‑143 (MQ=255) tGCAAAGAATTTGCAGCAGGTGGTTCTGGATCATGTCGCGCATCTGACCGGCTTTATCAAAATAGCCCCAGCGCCCTTCGATCCCCACTTCTTCTGCCACGGTAATCTCAACATGATCAATGGTGCGATTGTCCCAGTTATTc < 1:232789/143‑1 (MQ=255) tGCAAAGAATTTGCAGCAGGTGGTTCTGGATCATGTCGCGCATCTGACCGGATTTATCAAAATAGCCCCAGCGCCCTTCGATCCCCACTTCTTCTGCCACGGTAATCTCAACATGATCAATGGTGCGATTGTCACAGTTAtgc > 1:358528/1‑141 (MQ=255) gCAAAGCATTTGCCGCAGGTGGTTCTGGATCATGTCGCGCATCTGACCGGCTTTATCAAAATAGCCCCAGCGCCCTTCGATCCCCACTTCTTCTGCCACGGTAATCTCAACATGATCAATGGTGCGATTGTCCCAGTTATTca < 2:635763/143‑1 (MQ=255) tttCCAGCAGGTGGCTCCGGATCATGTCGCGCATCTGACCGGCTTTACCAAAATAGCCCCAGCGCCCTTCGATCCCCACTTCTTCTGCCACGGTAATCTCAACATGATCAATGGTGCGATTGTCCCAGTTATTCACAAACAgg < 1:30257/143‑1 (MQ=255) tGCAGCCGGTGGTCCTGGATCATGCCGCGCATCTGACCGGCTTTATCAAAACAGCCCCAGCGCCCGTCGATCCCCACTTCTTCTTACACGGTAAACTCAACATGATCAATGGTGCGATTGTCCCAGTTATTCACAAACAGGGa < 1:424885/143‑1 (MQ=255) gcagGTGGTTCTGGATCCTGTCGCGCATCTGCCCGGCTCTATCAAAATAGCCCCAGCGCCCTTCGATCCCCACTTCTTCTGCCACGGTAATCTCAATATGATCAATGGTGCGATTGTCCCAGTTATTCACAAACAGGGAGTTa < 2:307768/143‑1 (MQ=255) tCTGGATCATGTCGCGCATCTGCCCGGCTTTATCAAAATAGCCCCAGCGCCCTTCGATCCCCACTTCTTCTGCCACGGTAATCTCAACATGATCAATGGTGCGATTGTCCCAGTTATTCACAAACAGGGAGTTAGCAAAACGc < 2:495881/143‑1 (MQ=255) gTCGCGCATCTGACCGGCTTTATCAAAATAGCCCCAGCGCCCTTCGATCCCCACTTCTTCTGCCACGGTAATCTCAATATGATCAATGGTGCGATTGTCCCAGTTATTCACAAACAGGGAGTTAGCAAAACGCAGCGCCAACa < 1:454227/143‑1 (MQ=255) gcATCTGACCGGCTTTATCAAAATAGCCCCAGCGCCCTTCGATCCCCACTTCTTCTGCCACGGTAATCTCAACATGATCAATGGTGCGATTGTCCCAGTTATTCACAAACAGGGAGTTAGCAAAACGCAGCGCCAACAGGTTc < 1:207560/143‑1 (MQ=255) gcATCTGACCGGCTTTATCAAAATAGCCCCAGCGCCCTTCGATCCCCACTTCTTCTGCCACGGTAATCTCAACATGATCAATGGTGCGATTGTCCCAGTTATTCACAAACAGGGAGTTAGCAAAACGCAGCGCCAACAGGTTc < 1:557349/143‑1 (MQ=255) actcgACCGTCTTTATCAAACTACCCCCTGCGCCCTTCGACCCCCTCTTCTTCTCCCACCGTAATCTCAGTATGATCAAGTGTGCGATCGTCCCACTTATTCACAAACTGGGAGTTAGCAAAACGCAGCGCCAACAGGTTCAg < 2:346674/139‑1 (MQ=255) ccGGCTCTCTCAAAATAGCCCCAGCGCCCTTCGATCCCCACTTCTTCTGCCACGGTAATCTCAACATGATCAATGGTGCGATTGTCCCAGTTATTCACAAACAGGGAGTTAGCAAAACGCAGCGCCAACAGGTTCAGCACCGt < 2:466178/143‑1 (MQ=255) ggCTTTATCAAAATAGCCCCAGCGCCCTGCGATCCCCACTTCTTCTGCCACGGTAATCTCAATATGATCAATGGTGCGATTGTCCCAGTTATTCACAAACAGGGAGTTAGCAAAACGCAGCGCCAACAGGTTCAGCACCGttt < 1:203714/143‑1 (MQ=255) cTTTATCAAAATAGCCCCAGCGCCCTTCGATCCCCACTTCTTCTGCCACGGTAATCTCAACATGATCAATGGTGCCATTGTCCCAGTTATTCACAAACAGGGAGTTAGCAAAACCCAGCGCCCACCCGTTCAGCACCGTTTCt > 2:344768/1‑143 (MQ=255) tttATCAAAATAGCCCCAGCGCCCTTCGATCCCCACTTCTTCTGCCACGGTAATCTCAATATGATCAATGGTGCGATTGTGCCAGTTATTCACAAACAGGGAGTTAGCAAAACGCAGCGCCAACAGGTTCAGCACCGGTTCtt > 1:340087/1‑143 (MQ=255) tttATCAAAATAGCCCCAGCGCCCTTCGATCCCCACTTCTTCTGCCACGGTAATCTCAACATAATCAATGGTGCGATTGTCCCAGTTATTCACAAACAGGGAGTTAGCAAAACGCAGCGCCAACAGGTTCCGCACCGTTTCtt > 1:636722/1‑143 (MQ=255) aaaaTAGCCCCAGCGCCCTTCGATCCCCACTTCTTCTGCCACGGTAATCTCAATATGATCAATGGTGCGATTGTCCCAGTTATTCACAAACAGGGAGTTAGCAAAACGCAGCGCCAACAGGTTCAGCACCGTTTCTTTAccca > 2:245679/1‑141 (MQ=255) aaaTAGCCCCAGCGCCCTTCGATCCCCACTTCTTCTGCCACGGTAATCTCAATATGATCAATGGTGCGATTGTCCCAGTTATTCACAAACCGGGAGTTAGCAAAACGCAGCGCCAACAGGTTCAGCACCGTTTCTTTACCAAg > 1:187604/1‑143 (MQ=255) cccAGCGCCCTTCGCTCCTCACTTCTTCTGCCACGCTAATCTCAATATGATCAATGGTGCGATTGTCCCAGTTATTCACAAACAGGGAGTTAGCAAAACGCAGCGCCAACAGGTTCAGCACCGTTTCTTTACCAAGATAGTgg < 2:127387/143‑1 (MQ=255) ggcccccGTCGACCCCCCCTCCTCCTGCCCCGGTAGTCTCAACATGACCAAGGGTGCGATTGTCCCAGTTATTCCCACACAGGGAGTGAGCAAAACGCAGCGCCAACAGGTGCAGCACCGTTTCTTTACCAAGATAGTGGTCg < 1:59609/139‑1 (MQ=255) cgcgcCCTTCGACCCCCACTTCTGCTGCCACGGTAATCTCAACATGATCAATGGTGCGATTGTCCCAGTTATTCACAAACAGGGAGTTAGCAAAACGCAGCGCCAACAGGTTCAGCACCGTTTCTTTACCAAGATAGTGGTCg < 1:522970/142‑1 (MQ=255) aGCGCCCTTCGATCCCCACTTCTTCTGCCACGGTAATCTCAACATGATCAATGGTGCGATTGGCCCAGTTATTCACAAACAGGGAGTTAGCAAAACGCAGCGCCAACAGGTTCAGCACCGTTTCTTTACCAAGATAGTGGTCg < 2:405660/143‑1 (MQ=255) ttCGATCCCCACTTCCTCTGCCACGGTAATCTCAATATGATCAATGGTGCGATTGTCCCAGTTATTCACAAACAGGGAGTTAGCAAAACGCAGCGCCAACAGGTTCAGCACCGTTTCTTTACCAAGATAGTGGTCGATACGGt > 2:571611/1‑143 (MQ=255) tCGATCCTCACTTCTTCTGCCACGGTAATCTCAACATGATCAATGGTGCGATTGTCCCAGTTATTCACAAACAGGGAGTTAGCAAAACGCAGCGCCAACAGGTTCAGCACCGTTTCTCTACCAACATAGTGGTCGATACGGTa > 2:228142/1‑143 (MQ=255) ccccACTTCTTCTGCCACGGTAATCTCAACATGATCCATGTGGCGATTGTCCCAGTTATTCACAAACAGGGAGTTAGCAAAACGCATCGCCAACAGGTTCAGCACCGTTTCTTTACCAAGATAGTGGTCGATACGGTAAACCt > 2:448534/1‑143 (MQ=255) ccACTTCTTCTGCCACGGTAATCTCAACATGATCAATGGTGCGATTGTCCCAGTTATTCACAAACAGGGAGTTAGCAAAACGCAGCGCCAACAGGTTCAGCACCGTTTCTTTACCAAGATAGTGGTCGATACGGTAAACCTgg > 1:340166/1‑143 (MQ=255) ccACTTCTTCGGCCACGGTACTCTCAACATTCCCAATGGTGCGCTTGTCCCAGTTATTCACAAACAGGGAGTTAGCAAAACGCAGCGCCAACAGGTTCAGCACCGTTTCTTTACCAAGATAGTGGTCGATACGGTAAACCTgg < 1:536211/143‑1 (MQ=255) ccttcttCTGCCACGGTATTCTCATCAGGATGAATGGTGGGATTGTCCCAGTTATTCACAAACAGGGAGTTAGCAAAACGCAGCGCCAACAGGTTCAGCACCGTTTCTTTACCAAGATAGTGGTCGATACGGTAAACCTGGCa < 2:635105/142‑1 (MQ=255) cACGGTAATCTCAACATGATCAATGGTGCGATTGTCCCAGTTATTCACAAACAGGGAGTTAGCAAAACGCAGCGGCAACAGGTTCAGCACCGTTTCTTTACCAAGATAGTGGTCGATACGGTAACCCTGGCACTCCTCGAAGt > 2:422064/1‑143 (MQ=255) cAACATGATCAATGGTGCGATTGTCCCAGTTATTCACAAACAGGGAGTTAGCAAAACGCAGCGCCAACAGGTTCAGCACCGTTTCTTTACCAAGATAGTGGTCGATACGGTAAACCTGGCACTCCTCGAAGTATTCGCCAAcc > 1:209807/1‑143 (MQ=255) | CAGGTCAGACGGCGGAGACATCGCAATCATGCAAAGAATTTGCAGCAGGTGGTTCTGGATCATGTCGCGCATCTGACCGGCTTTATCAAAATAGCCCCAGCGCCCTTCGATCCCCACTTCTTCTGCCACGGTAATCTCAACATGATCAATGGTGCGATTGTCCCAGTTATTCACAAACAGGGAGTTAGCAAAACGCAGCGCCAACAGGTTCAGCACCGTTTCTTTACCAAGATAGTGGTCGATACGGTAAACCTGGCACTCCTCGAAGTATTCGCCAACC > NC_000913/1935550‑1935829 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |