| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_000913 | 2,356,738 | G→A | 12.2% | intergenic (+27/‑166) | glpC → / → rpnE | anaerobic glycerol‑3‑phosphate dehydrogenase subunit C/inactive recombination‑promoting nuclease‑like protein RpnE |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 2,356,738 | 0 | G | A | 12.2% | 71.4 / 2.2 | 31 | intergenic (+27/‑166) | glpC/rpnE | anaerobic glycerol‑3‑phosphate dehydrogenase subunit C/inactive recombination‑promoting nuclease‑like protein RpnE |
| Reads supporting (aligned to +/- strand): ref base G (13/14); new base A (2/2); total (15/16) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.41e-01 | |||||||||||
GATCTGGTGGTCACCGACTGCGAAACCTGTAAATGGCAGATTGAGATGTCCACAAGTCTTCGCTGCGAACATCCGATTACGCTACTGGCCCAGGCGCTGGCTTAAACTCCTTTCTGATGCCCGGTAAGCATGTGGTTACCGGGCATTTTTGCGTACACGATTCCGTGCCCAATGTATGCGTTGCAACGCAGTGAAAATTCCTCTGAAAACGTCTCGCAAAGGCTGAAACTGGCAGATGTCAAAGGCCTGGGATAACCGTAATGTCGCGT > NC_000913/2356607‑2356875 | tgtccggtggtCACCGTCTGCGACACCGTGAAGTGCCAGACTGAGATGGCCACATGTCTTCGCTGCTATCTTCCGATTTCGCTACTGGCCCAGGCGTTGGCTTAAACTCCTTTCTGATGCCCGGTACCCACATGCTTACCggg < 2:534374/138‑1 (MQ=255)gATCTGGTGGTCACCGACTGCGAAACCTGTAAATGGCAGATTGAGATGTCCACAAGTCTTCGCTGCGAACATCCGATTACGCTACTGGCCCAGGCGCTGGCTTAAACTCCTTTCTGATGCCCGGTAACCACATGCTTACCggg < 1:436846/143‑1 (MQ=255) tggtggTCACCGACTGCGAAACCTGTAAATGGCAGATTGAGATGTCCACAAGTCTTCGCTGCGAACATCCGATTACGCTACTGGCCCAGGCGCTGGCTTAAACTCCTTTCTGATGCCCGGTAAGCATGTGGGTACCGGGCAtt > 2:173916/1‑143 (MQ=255) gtggtCGCCGGCTGCGAGGCCGGTGAGTGGCAGATTGGGATGTCGACAGGTCTTCGCTGCGAACATCCGATGACGCTACTGGCCCAGGCGCTGGCTTAAACTCCTTTCTGATGCCCGGTAAGCATGTGGTTACCGGGCAtttt < 2:225204/143‑1 (MQ=255) tggtCACCGACTGCGAAACCTGTAAATGGCAGATTGAGATGTCCACACGTCTTCGCTGCGAACATCCGATTACGCTACTGGCCCAGGCGCTGGCTTAAACTCCTTTCTGATGCCCGGTAAGCATGTGGTTACCGGGCAttttt < 1:254661/143‑1 (MQ=255) tggtCACCGACTGCGAAACCTGTAAATGGCAGATTGAGATGTCCACAAGTCTTCGCTGCGAACATCCGATTACGCTACTGGCCCAGGCGCTGGCTTAAACTCCTTTCTGATGCCCGGTAAGCATGTGGTTACCGGGCAttttt < 1:572279/143‑1 (MQ=255) ggtCACCGACTGCGAAACCTGTAAATGGCAGATTGAGATGTCCACAAGTCTTCGCTGCGAACATCCGATTACGCTACTGGCCCAGGCGCTGGCTTAAACTCCTTTCTGATGCCCGGTAAGCATGTGGTTACCGGGCATTTTTg < 2:391677/143‑1 (MQ=255) gtCAGCGACCGCGAACCCTGTGAATGGCAGAGTGAGAGGTCCACAAGTCTGCGCTGCGAACATCCGATTACGCTACTGGCCCAGGCGCTGGCTAAAACTCCTTTCTGATGCCCGGTAAGCATGTGGTTACCGGGCATTTTTGc < 1:93506/143‑1 (MQ=255) gtCACCGACTGCGAAACCTGTAAATGGCAGAGTGAGATGTCCACAAGTCTTCGCTGCGCACATCCGATTACGCTACTGGCCCAGGCGCTGGCTTAAACTCCTTTCTGATGCCCGGTAAGCATGTGGTTACCGGGCATTTCTGc > 1:664917/1‑143 (MQ=255) tCACCGACTGCGAAACCTGTAAATGGCAGATTGAGATGTCCACAAGTCTTCGCTGCGAACATCCGATTACGCTACTGGCCCAGGCGCTGGCTTAAACTCCTTTCTGATGCCCGGTAAGCATGTGGTTACCGGGCATTTTTGCg > 2:462758/1‑143 (MQ=255) gACTGCGAAACCTGTAAATGGCAGATTGAGATGTCCACAAGTCTTCGCTGCGAACATCCGATTACGCTACTGGCCCAGGCGCTGGCTTAAACTCCTTTCTGATGCCCGGTAAGCATGTGGTTACCGGGCATTTTTGCGTacac < 2:621843/143‑1 (MQ=255) gACTGCGAAACCTGTAAATGGCAGATTGAGATGTCCACAAGTCTTCGCTGCGAACATCCGATTACGCTACTGGCCCAGGCGCTGGCTTAAACTCCTTTCTGATGCCCGGTAAGCATGTGGTTACCGGGCATTTTTGCGTacac > 2:198602/1‑143 (MQ=255) tGTAAATGGCAGATTGAGATGTCCACAAGTCTTCGCTGCGAACATCCGATTACGCTACTGGCCCAGGCGCTGGCTTAAACTCCTTTCTGATGCCCGGTAAGCATATGGTTACCGGGCATGTTTGCGGACAAGAGTCCATGccc > 1:392924/1‑143 (MQ=255) acaAGTCTTCGCTGCGAACATCCGATTACGCTACTGGCCCAGGCGCTGGCTTAAACTCCTTTCTGATGCCCGGTAAGCATGTGGTTACCGGGCCTTTTTGCGTACACGCTTCCGTGCCCAATGTATGCGTTGCAACGCAGTGa > 1:580412/1‑143 (MQ=255) acaAGTCTTCGCTGCGAACATCCGATTACGCTACTGGCCCAGGCGCTGGCTTAAACTCCTTTCTGATGCCCGGTAAGCATGTGGTTACCGGGCATTTTTGCGTACACGATTCCGTGCCCAATGTATGCGTTGCAACGCAGTGa > 1:51255/1‑143 (MQ=255) tCTTCGCTGCGAACATCCGATTACGCTACTGGCCCAGGCGCTGGCTTAAACTCCTTTCTGATGCCCGGTACGCATGTGGTTACCGGGCATTTTTGCGTACACGCTTCCGTGCCCAATGTTTCCATTGCAACGCAGTGAACAtt > 2:467369/1‑143 (MQ=255) cTTCGCTGCGAACATCCGATTACGCTACTGGCCCAGGCGCTGGCTTAAACTCCTTTCGGATGCCCGGTAAGCATGTGGTTACCGGGCATTTTTGCGGAAAGGAATCCGTGCCCAATGTATGCGTTGCAACGCAGTGAAAATTc < 1:128954/143‑1 (MQ=255) tCGCTGCGAACATCCGATTACGCTACTGGCCCAGGCGCTGGCTTAAACTCCTTTCTGATGCCCGGTAAGCATGTGGTTACCGGGCATTTTTGCGTACACGATTCCGTGCCCAATGTATGCGTTGCAACGCAGTGAACATTCct > 1:629691/1‑143 (MQ=255) cGCTGCGAACATCCGATTACGCTACTGGCCCAGGCGCTGGCTTAAACTCCTTTCTGATGCCCGGTAAGCATGTGGTTACCGGGCATTTTTGCGTACACGATTCCGTGCCCAATGTATGCGTTGCAACGCAGTGAAAATTCctc > 1:318287/1‑143 (MQ=255) gATTACGCTACTGGCCCAGGCGCTGGCTTAAACTCCTTTCTGATGCCCGGTAAGCATGTGGTTACCGGGCATTTTTGCGTCCACGCTTCCGTGCCCAATGTATGCGTTGCAACGCAGTGAAAATTCCTCTGCAAACGTCTCGc > 2:295280/1‑143 (MQ=255) aTTACGCTACTGGCCCAGGCGCTGGCTTAAACTCCTTTCTGATGCCCGGTAAGCATGTGGTTACCGGGCATTTTTGCGTACACGATTCCGTGCCCAATGTATGCGTTGCAACGCAGTGAAAATTCCTCTGAAAACGTCTCGCa > 1:371924/1‑143 (MQ=255) tGGCCCAGGGGCTGTCGTAACCGCTGTTCTGAGGCCGGGTAATCATGTGGTTACCGGGCATTTTTGCGTCCACGATTCCGTGCCCAATGTATGCGTTGCAACGCAGTGAAAATTCCTCTGAAAACGTCTCGCAAAGGCTGaaa < 2:207516/143‑1 (MQ=255) ggCCCAGGCGCTGGCGTAAACTCCTTTCTGATGCCCGGTAAGCATGTGGTTACCGGGCATTTTTGCGTACACGATTCCGTGCCCAATGTATGCGTTGCAACGCAGTGAAAATTCCTCTGAAAACGTCTCGCAAAGGCTGAAAc < 1:98469/143‑1 (MQ=255) cAGGCGCTGGCTTAAACTCCTTTCTGATGCCCGGTAAGCATGTGGTTACCGGGCATTTTTGCGTACACGATTCCGTGCCCAATGTATGCGTTGCAACGCAGTGAAAATTCCTCTGAAAACGTCTCGCAAAGGCTGAAACTGGc < 1:198602/143‑1 (MQ=255) gcgtTGGCTTAAACTCCTTTCTTATGCCCGGTAAGTATGTGGTTACCGGGCATTTTTGCGTACACGATTCCGTGCCCAATGTATGCGTTGCAACGCAGTGAAAATTCCTCTGAAAACGTCTCGCAAAGGCTGAAACTGGCAGa < 1:83753/143‑1 (MQ=255) tAAACTCCTTTCTGATGCCCGGTAAGCATGTGGTTACCGGGCATTTTTGCGTACACGATTCCGTGCCCAATGTATGCGTTGCAACGCAGTGAAAATTCCTCTGAAAACGTCTCGCAAAGGCTGAAACTGGCAGATGTCACAgg > 1:82931/1‑143 (MQ=255) ctctTTTCTGATGCCCGGTAAGCATGTGGTTCCCGGGCATTTTTACGTACACGATTCCGTGCCCAATGTATGCGTTGCAACGCAGTGAAAAGTCCTCTGAAAACGTCTCGCAAAGGCTGAAACTGGCAGATGTCAAAGGCCTg < 2:28637/143‑1 (MQ=255) tCCTTTCTGATGCCCGGTAAGCATGTGGTTACCGGGCATTTTTGCGTACACGATTCCGTGCCCAATGTATGCGTTGCAACGCAGTGAAAATTCCTCTGAAAACGTCTCGCAAAGGCTGAAACTGGCAGATGTCAAAGGCCTgg > 2:646722/1‑143 (MQ=255) tttCTGGTGCCCGGTAGGTATGTGGTTACCGGGCATTTTTGCGTACACGATTCCGTGCCCGATGTATGCGTTGCAACGCAGTGAAAATTCCTCTGAAAACGTCTCGCAAAGGCTGAAACTGGCAGATGTCAAAGGCCTGGGAt < 2:363002/143‑1 (MQ=255) gCCCGGTAACCACATGCTTACCGGGCATTTTTGCGTACACGATTCCGTGCCCAATGTCTGCGTTGCAACGCAGTGAAAATTCCTCTGAAAACGTCTCGCAAAGGCTGAAACTGGCAGCTGTCAAAGGCCCGGGAACACCGtca > 2:421429/1‑141 (MQ=255) cgCATGTGGTTACCGGGCAGTTTTGCGTACACGATTCCGTGCCCAATGTATGCGTTGCAACGCAGTGAAAAGTCCTCTGAAAACGGCTCGCAAAGGCTGAAACTGGCAGATGTCAAAGGCCTGGGATAACCGTAATGTCGCGt < 2:207466/142‑1 (MQ=255) | GATCTGGTGGTCACCGACTGCGAAACCTGTAAATGGCAGATTGAGATGTCCACAAGTCTTCGCTGCGAACATCCGATTACGCTACTGGCCCAGGCGCTGGCTTAAACTCCTTTCTGATGCCCGGTAAGCATGTGGTTACCGGGCATTTTTGCGTACACGATTCCGTGCCCAATGTATGCGTTGCAACGCAGTGAAAATTCCTCTGAAAACGTCTCGCAAAGGCTGAAACTGGCAGATGTCAAAGGCCTGGGATAACCGTAATGTCGCGT > NC_000913/2356607‑2356875 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |