| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_000913 | 2,447,639 | G→A | 100% | H268Y (CAT→TAT) | prmB ← | 50S ribosomal subunit protein L3 N(5)‑glutamine methyltransferase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 2,447,639 | 0 | G | A | 92.6% | 38.5 / ‑4.7 | 22 | H268Y (CAT→TAT) | prmB | 50S ribosomal subunit protein L3 N(5)‑glutamine methyltransferase |
| Reads supporting (aligned to +/- strand): ref base G (1/1); major base A (8/10); minor base C (2/0); total (11/11) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.74e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.89e-01 | |||||||||||
| Rejected as polymorphism: E-value score below prediction cutoff. | |||||||||||
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. | |||||||||||
CGTGTTTACTTAATCTTTATAAATCGCGAAATGTTCTCGTGCGGCAATAAGCTGCTCTTTGGTGAGCATAAACACACCATCGCCGCCGTTATCAAACTCCAGCCAGGTGAACGGAACATCCGGATATTGTTCCATAAGATGTACCATGCTGTTGCCGACTTCACAAATCAACACGCCATCATCAGCAAGGTAATCTGCCGCGTTACCGAGAATGCGACGCGTCAGTTTCAGGCCGTCAGTGCCAGATGCCAGGCCCAGTTCCGGCTCGTGGCGGTATTCGT > NC_000913/2447499‑2447779 | cgttgttACCTAATCTGTATAAATCGCGAAGTGTTCTCGTGCGGCAATAAGCTGCTCTTTGGTGAGCATAAACACACCATCGCCGCCGTTATCAAACTCCAGCCAGGTGAACGGAACATCCGGATATTGTTCCATAAGAtata < 2:360058/138‑1 (MQ=255) ttAATCTTTATAAATCGCGAAATGTTCTCGTGCGGCAATAAGCTGCTCTTTGGTGAGCATAAACACACCATCGCCGCCGTTATCAAACTCCAGCCAGGCGAACGGAACATCCGGATATTGTTCCATAAGATCTACCATGCTGt > 2:372145/1‑143 (MQ=255) tAAGCTTTATAAATCGCGAGATGTTCTCGTGCGGCAATAAGCTGCTCTTTGGTGAGCATAAACACACCATCGCCGCCGTTATCAAACTCCAGCCAGGTGAACGGAACATCCGGATATTGTTCCATAAGATATACCATGCTGtt < 2:419865/143‑1 (MQ=255) taAATCGCGAAATGTTCTCGTGCGGCAATAAGCTGCTCTTTGGTGAGCATAAACACACCATCGCCGCCGTTATCAAACTCCAGCCAGGTGAACGGAACATCCGGATATTGTTCCATAAAATGTACCATCCTCTTGCCGACTTc > 1:617959/1‑143 (MQ=255) gcgAAATGTTCTCGTGCGGCAATAAGCGGCTCTTTGGTGAGCATAAACACACCATCGCCGCCGTTATCAAACTCCAGCCAGGTGAACGGAACATCCGGATATTGTTCCATAAGATATACCATGCTGTTGCCGACTTCACAAAt < 1:220115/143‑1 (MQ=255) aaaTGTTCTCGTGCGGCAATAAGCTGCTCTTTGGTGAGCATAAACACACCATCGCCGCCGTTATCAAACTCCAGCCAGGTGAACGGAACATCCGGATATTGTTCCATAAGATATACCATGCTGTTGCCGACTTCACAAATCaa > 1:128613/1‑143 (MQ=255) tGTTCTCGTGCGGCAATAAGCTGCTCTTTGGTGAGCATAAACACACCATCGCCGCCGTTATCAAACTCCAGCCAGGTGAACGGAACATCCGGATATAGCTCCATAAGATATACCATGCTGTTGCCGACCTCACAACTccagcc > 2:435443/1‑138 (MQ=255) tGTTCTCGTGCGGCAATAAGCTGCTCTGTGGTGAGCATAAACACACCATCGCCGCCGTTATCAAACTCCAGCCAGGTGAACGGAACATCCGGATATTGTTCCATAAGATATACCATGCTGTTGCCGACTTCACAAATCAacac < 1:323202/143‑1 (MQ=255) gCGGCAATAAGGTGCTCGTTGGTGAGCATAAACACACCATCGCCGCCGTTATCAAACTCCAGCCAGAAGAACGGAACACCCGGATATTGTTCCATAAGAAATACCACGCTGGTGCCGACTTCACAAATCAACACGCcaccctg > 2:631641/1‑138 (MQ=255) ctgctCTTTGGTGAGCATAAACACACCATCGCCGCCGTTATCAAACTCCAGCCAGGTGAACGGAACATCCGGATATTGTTCCATAAGATCTACCATGCTGTTGCCGACTTCCAAAATCAACACGCAATCACCAGCAAGGTacc > 2:543169/1‑141 (MQ=255) tGGTGAGCATAAACACACCATCGCCGCCGTTATCAAACTCCAGCCAGGTGAACGGAACATCCGGATATTGTTCCATAAGATATACCATGCTGTTGCCGACTTCACAAATCAACACGCCATCATCAGCAAGGTAATCTGCcgcg < 1:346355/143‑1 (MQ=255) cgTTATCAAATGCCAGCCAGTTAAAGGTAACATCCGGATAGTGTTCCATAAGATATACCATGCTGTTGCCGACTTCACAAATCAACACGCCATCATCAGCAAGGTAATCTGCCGCGTTACCGAGAATGCGACGCGTCAGTTTc < 2:380301/143‑1 (MQ=255) gTTATCAAACTCCAGCCAGGTGAACGGAACATCCGGATATTGTTCCATAAGATATACCATGCTGTTGCCGACTTCACAAATCAACACGCCATCATCAGCAAGGTAATCTGCCGCGTTACCGAGAATGCGACGCGTCGGTTTCa > 1:2148/1‑143 (MQ=255) ttATCAAACGCCAGCCACGTGAACGGAACCTCCGGATATTGTTCCATAAGATATACCATGCTGTTGCCGACGTCACAAATCAACACGCCATCATCAGCAAGGTAATCTGCCGCGTTACCGAGAATGCGACGCGTCAGTTTCAg < 2:141302/143‑1 (MQ=255) gAACGGAACATCCGGATATTGTTCCATAAGATATACCATGCTGTTGCCGACTTCACAAATCAACACGCCATCATCAGCAAGGTAATCTGCCGCGTTACCAGGAATGCGACGCGGCAGTTTCAGGCGGGCAGTGCCCGAGGCCa > 2:537705/1‑143 (MQ=255) cGGAACATCCGGATATTGTTCCATAAGATATACCATGCTGTTGCCGACTTCACAAATCAACACGCCATCATCAGCAAGGTAATCTGCCGCGTTACCGAGAATGCGACGCGTCCGTTTCAGGCCGTCCGTCCCAGATGCCAGGc > 2:41376/1‑143 (MQ=255) cGGAACATCCGGATATTGTTCCATAAGATATACCATGCTGTTGCCGACTTCACAAATCAACACGCCATCATCAGCAAGGTAATCTGCCGCGTTACCGAGAATGCGACGCGGCAGTTGCAGGCCGTCAGTGCCAGGTGCGAGGc > 2:41408/1‑143 (MQ=255) ggAACATCCGAATATTGTTCCATAAGATATACCATGCTGCTGCCGACTTCACAAATCACCACGCCAGCATCATCAAGGTAATCTGCCTCGTTACCGAGAATGCGACGCGTCAGTTTCAGGCCGTCAGTGCCAGATGCCAGGcc < 1:480994/143‑1 (MQ=255) aTCCGGAGATTGTTCCATAAGTGATACCATGCTGTTGCCGACTTCACAAATCAACACGCCAGCATCAGCAAGGTAATCTGCCGCGTTACCGAGAATGCGACGCGTCAGTTTCAGGCCGTCAGTGCCAGATGCCAGGCCCAGtt < 1:581522/143‑1 (MQ=255) ccGGGTATTGTGCCATCTGGTATTCCATGCTGTTGCCGACTTCCCACATCACCACGCCATCTTCTGCGAGGTGGTCTGCCGCGTTACCGAGAATGCGACGCGTCAGTTTCAGGCCGTCAGTGTCAGATGCCAGGCCCAGTTcc < 2:283053/143‑1 (MQ=255) gATATTGTTCCATAAGATATACCATGCTGTTGCCGACTTCACAAATCAACACGCCATCATCAGCAAGGTAATGTGCCGCGTTACCGAGCATGCGCCGCGGCGGTGTCAGGCCGTCAGTGCGAGATGCCGAGCCCAGTTCCggg > 2:207297/1‑142 (MQ=255) tCCATAAGATATACCATGCTGGTGCCGACTTCACAAATCAACACGCCATCATCAGCAAGGTAATCTGCCGCGTTACCGAGAATGCGACGCGTCAGTTTCAGGCCGTCAGTGCCAGATGCCAGGCCCAGTTCCGGCTCGTGGCg < 1:66511/143‑1 (MQ=255) aTGTGCCATGCGTTCGCCCAGTTCACAAGTCCACACGCCCTCCTCAGCAAGGTAATCTGCCGCGTTACCGAGAATGCGACGCGTCAGTTTCAGGCCGTCAGTGCCAGATGCCAGGCCCAGTTCCGGCTCGTGGCGGTATTCGt < 2:428776/143‑1 (MQ=255) | CGTGTTTACTTAATCTTTATAAATCGCGAAATGTTCTCGTGCGGCAATAAGCTGCTCTTTGGTGAGCATAAACACACCATCGCCGCCGTTATCAAACTCCAGCCAGGTGAACGGAACATCCGGATATTGTTCCATAAGATGTACCATGCTGTTGCCGACTTCACAAATCAACACGCCATCATCAGCAAGGTAATCTGCCGCGTTACCGAGAATGCGACGCGTCAGTTTCAGGCCGTCAGTGCCAGATGCCAGGCCCAGTTCCGGCTCGTGGCGGTATTCGT > NC_000913/2447499‑2447779 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |