| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_000913 | 2,917,603 | T→G | 100% | V849V (GTT→GTG) | barA → | sensory histidine kinase BarA |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 2,917,603 | 0 | T | G | 98.2% | 74.0 / ‑6.4 | 29 | V849V (GTT→GTG) | barA | sensory histidine kinase BarA |
| Reads supporting (aligned to +/- strand): ref base T (0/0); major base G (14/14); minor base A (0/1); total (14/15) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.78e-01 | |||||||||||
| Rejected as polymorphism: E-value score below prediction cutoff. | |||||||||||
| Rejected as polymorphism: Frequency below/above cutoff threshold. | |||||||||||
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. | |||||||||||
TGAACCCGAATGCGACCCTCGACTGGCAACTGGCACTACGCCAGGCAGCAGGAAAAACCGATTTAGCGCGCGATATGCTGCAAATGTTACTCGATTTCCTGCCTGAAGTTCGCAACAAAGTTGAGGAACAGCTGGTTGGAGAAAACCCGGAAGGCCTGGTTGATTTGATTCATAAACTGCATGGCAGTTGC‑GGCTATAGCGGTGTGCCACGTATGAAGAATCTCTGCCAACTGATCGAACAACAGCTACGTAGCGGTACTAAAGAAGAAGATT > NC_000913/2917467‑2917739 | tgAACCCGGATGCGAGCCACGAGTGGCAACAGGCAATACGCCGGGCAGCAGGAAAAACCGGTTTAGCGCGGGAGATGCTGCAAATGTTACTCGATTTCCTGCCTGAAGTTCGGAACAAAGTGGAGGAACAGCTGGTGGGAGaa < 2:275354/143‑1 (MQ=255)tgAACCCGAATGCGACCCTCGACTGGCAACTGGCACTACGCCAGGCAGCAGGAAAAACCGATTTAGCGCGCGATATGCTGCAAATGTTACTCGATTTCCTGCCTGAAGTTCGCAACAAAGTTGAGGAACAGCTGGTGGGAGaa > 2:601345/1‑143 (MQ=255) actggcaactggcaCTGCGCCAGGCAGCAGGAAAAACCGATTTAGCGCGCGATATGCTGCAAATGTTACTCGATTTCCTGCCTGAAGTTCGCAACAAAGTTGAGGAACAGCTGGTGGGAGAAAACCCGGAAGGCCTGGttgat < 2:406585/143‑1 (MQ=255) ggaactggctCGACGCGAGGCAGCAGGGAAAACCGATTTGGCGCGCGATATGCTGCAAATGTTACTCGATTTCCTGTCTGAAGTTCGCAACAAAGTTGAGGAACAGCTGGTGGGAGAAAACCCGGAAGGCCTGGttgatttga < 2:481743/141‑1 (MQ=255) gcaactggcaCTACGCCAGGCAGCAGGAAAAACCGATTTAGCGCGCGATATGCTGCAAATGTTACTCGAATTCCTGCCTGAAGTTCGCAACAAAGTTGAGGAACAGCCGGTGGGAGAAAACCCGGAAGGCCCGGacgatttga > 2:368488/1‑143 (MQ=255) caactggcaCTACGCCAGGCAGCAGGAAAAACCGATTTAGCGCGCGATATGCTGCAAATGTTACTCGATTTCCTGCCTGAAGTTCGCAACAAAGTTGAGGAACAGCTGGTGGGAGAAAACCCGGAAGGCCTGGttggtttggt > 1:5878/1‑141 (MQ=255) aCGTCAGGCAGGAGGAAAAAGCGATTGAGCGCGCGATATGCTGCAAATGTTACTCGATTTCCGGCCTGAAGTTCGCAACAAAGTTGAGGAACAGCTGGTGGGAGAAAACCCGGAAGGCCTGGTTGATTTGATTCATAAACTGc < 2:645131/143‑1 (MQ=255) ggAAAAACCGATTTAGCGCGCGATATGCTGCAAATGTTACTCGATTTCCTGCCTAAAGTTCGCAACAAAGTTGAGGAACAGCTGGTGGGAGAAAACCCGGAAGACCAGGTAGACTAGATTCGTAGACTGCATGGAAGttacag > 2:321372/1‑139 (MQ=255) gAAAAACTGAGTTAGCGCGCGATATGCTGCAAATGTTACTCGATTGCCTGCCTGAAGTTCGCAACAAAGTTGAGGAACAGCTGGTGGGAGAAAACCCGGAAGGCCTGGTTGATTTGATTCATAAACTGCATGGCAGTTGC‑GGc < 2:617702/143‑1 (MQ=255) gAAAAACCGATTTAGCGCGCGATATGCTGCAAATGTTACTCGATTTCCTGCCTGAAGTTCGCAACAAAGTTGAGGAACAGCTGGTGGGAGAAAACCCGGAAGGCCTGGTTGATTTGATTCATAAACTGCATGGCGGTTGC‑GGc > 1:277042/1‑143 (MQ=255) aCCGATTTAGCGCGCGATATGCTGCAAATGTTACTCGATTTCCTGCCTGAAGTTCGCAACAAAGTTGAGGAACAGCTGGTGGGAGAGAACCCGGAAGGCCTGGCTGATTTTATTCATAAACTGCATAGCAGTGGC‑GGCGATAg > 2:346473/1‑143 (MQ=255) aCCGATTTAGCGCGCGATATGCTGCAAATGTTACTCGATTTCCTGCCTGAAGTTCGCAACAAAGTTGAGGAACAGCTGGTGGGAGAAAACCCGGAAGGCCTGGTTGATTTGATTCATAAACTGCATGGCAGTTGC‑GGCTATAg < 1:601345/143‑1 (MQ=255) cGATTTAGCGCGCGATATGCTGCAAATGTTACTCGATTTCCTGCCTGAAGTTCGCAACAAAGTTGAGGAACAGCTGGTGGGAGAAAACCCGGAAGGCCTGGTTGATTTGATTCATAAACTGCATGGCAGTTGC‑GGCTATAGCg > 1:227583/1‑143 (MQ=255) cGATTTACCGTGGGCTATGCTGCAAATGTTACGCGGTTTCCTGCCTGAAGTTCGCAACAAAGTTGAGGAACAGCTTGTGGGAGAAAACCCGGAAGGCCTGGTTGATTTGATTCATAAACTGCATGGCAGTTGC‑GGCTATAGCg < 2:422566/143‑1 (MQ=255) gcgATATGCTGCAAATGTTACTCGATTTCCTGCCTGAAGTTCGCAACAAAGCTGAGGAACAGCTGGTGGGAGAAAACCCGGAAGGCCTGGTTGATTTGATTCATAAACTGCATGGCAGTTGC‑GGCTATCGCGGGGTGCCACgg > 1:6806/1‑142 (MQ=255) gATATGCTGCAAATGTTACTCGATTTCCTGCCTGAAGTTCGCAACAAAGTTGAGGAACAGCTGGTGGGAGAAAACCCGGAAGGCCTGGTTGATTTGATTCATAAACTGCATGGCAGTTGC‑GGCTATAGCGGTGTGCCACGTAt > 1:445146/1‑143 (MQ=255) aaTGTTACTCGATTTCCTGCCTGAAGTTCGCAACAAAGTTGAGGAACAGCTGGTGGGAGAAAACCCGGAAGGCCTGGTTGATTTGATTCATCCACTGCATGTCAGTTTC‑GGCTATAGCGGTGTGCCACGTATGAAGAATctct < 1:644332/143‑1 (MQ=255) aaTGGTAGTCGCTTTCCTGCCTGAAGTGAGCACCAAAGTTGAGGACAAGAAGGAAGGAAAAAAACGGGTAGAACTGTTTAATTTGGTTCGTAAACTGAATCG‑GGTTACTTGCTACGGCGGTGTGCCACGTATGAAGAATctct < 2:448709/143‑1 (MQ=255) tGTTACTCGATTTCCTGCCTGAAGTTCGCAACAAAGTTGAGGAACAGCTGGTGGGAGAAAACCCGGAAGGCCTGGTTGATTTGATTCATAAACTGCATGGCAGTTGC‑GGCTATAGCGGTGTGCCACGTATGAAGAATCTCTGc < 1:379071/143‑1 (MQ=255) cTCGATTTCCTGCCTGAAGTTCGCAACAAAGTTGAGGAACAGCTGGTGGGAGAAAACCCGGAAGGCCTGGTTGATTTGATTCATAAACTGCATGGCAGTTGC‑GGCTATAGCGGTGTGCCACGTATGAAGAATCTCTGCCAACt > 1:577985/1‑143 (MQ=255) tCGATTTCCTGCCTGAAGTTCGCAACAAAGTTGAGGAACAGCTGGTGGGAGAAAACCCGGAAGGCCTGGTTGGTTTGATTCATAAACTGCATGGCGGTTGC‑GGCTATAGCGGTGTGCCGCGTATGAAGAATCTCTGCCGAcgg > 1:142281/1‑141 (MQ=255) gATTTCCTGCCTGAAGTTCGCAACAAAGTTGAGGACCAGCTGGTGGGAGAAAACCCGGAAGGCCTGGTTGATTTGATTCATAAACTGCATGGCAGTTGC‑GGCTATAGCGGTGTGCCACGCATGAAGAATCTCTGCCCACCGAt > 1:128538/1‑143 (MQ=255) gATCTCATGCCGGAAGATCCCAGCATAGGTGAGGAACAGCTGGTGGGAGAAAACCCGGAAGGCCTGGTTGATTTGATTCATAAACTGCATGGCAGTTGC‑GGCTATAGCGGTGTGCCACGTATGAAGAATCTCTGCCAACTGAt < 2:33011/143‑1 (MQ=255) tCGCAACAAAGTTGAGGAACAGCTGGTGGGAGAAAACCCGGAAGGCCTGGTTGATTTGATTCATAAACTGCATGGCAGTTGC‑GGCTATAGCGGTGTGCCACGTATGAAGAATCTCTGCCAACTGATCGAACAACAGCTACGTa > 2:128900/1‑143 (MQ=255) gCAACAAAGTTGAGGAACAGCTGGTGGGAGAAAACCCGGAAGGCCTGGTTGATTTGATTCATAAACTGCATGGCAGTTGC‑GGCTATAGCGGTGTGCCACGTATGAAGAATCTCTGCCAACTGATCGAACAACAGCTCCGTAGc > 1:176370/1‑143 (MQ=255) tGAGGAACAGCTGGTGGGAGAAAACCCGGAAGGCCTGGTTGATTTGATTCATAAACTGCATGGCAGTTGC‑GGCTATAGCGGTGTGCCACGTATGAAGAATCTCTGCCAACTGATCGAACAACAGCTACGTAGCGGTACTAaag < 1:435350/143‑1 (MQ=255) ggaCAGCTGGTGGGGGAAGACCCGGAAGGCCGGGTTGATTTGATTCATAAACTGCATGGCAGTTGC‑GGCTATAGCGGTGTGCCACGTATGAAGAATCTCTGCCAACTGAGCGAACAACAGCTACGTAGCGGTACTAaagaaga < 1:176497/141‑1 (MQ=255) cAGCGGGTGGGAGAAAACCCGGAAGGCCTGGTTGATTTGATTCATAAACTGCATGGCAGTTGC‑GGCTATAGCGGTGTGCCACGTATGAAGAATCCCTGCCCAATGACCGAACAACAGCTACGTAGCGGTACTAAagaagaaga < 2:483498/143‑1 (MQ=255) gCTGGTGGGACAAAACCCGGAAGGCCTGGTTGATTTGATTCATAAACTGCATGGCAGTTGC‑GGCTATACCGGTGTGCCACGTATGAAGAATCTCTGCCAACTGATCGAACAACAGCTACGTAGCGTTACTAAACAAGAAGAtt < 2:207540/143‑1 (MQ=255) | TGAACCCGAATGCGACCCTCGACTGGCAACTGGCACTACGCCAGGCAGCAGGAAAAACCGATTTAGCGCGCGATATGCTGCAAATGTTACTCGATTTCCTGCCTGAAGTTCGCAACAAAGTTGAGGAACAGCTGGTTGGAGAAAACCCGGAAGGCCTGGTTGATTTGATTCATAAACTGCATGGCAGTTGC‑GGCTATAGCGGTGTGCCACGTATGAAGAATCTCTGCCAACTGATCGAACAACAGCTACGTAGCGGTACTAAAGAAGAAGATT > NC_000913/2917467‑2917739 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |