Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,962,562 T→C 13.2% N68S (AAC→AGC) ‡ ppdC ← putative YgdA

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,962,5620TC13.2% 67.5 / 4.0 34N68S (AAC→AGC) ‡ppdCputative YgdA
Reads supporting (aligned to +/- strand):  ref base T (14/15);  new base C (3/2);  total (17/17)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.80e-01

CCTTAACATAGCGGCTCCTGACTACTGACGATTCGGGCAATGCAGGCGGGTCATCTCGCCTTCTCTGCCCCCGGGTGAAACTAGCGTAACGCTGATGCTGACACATCCCGCCTGCGATGTCTGCATTCGGTTGACCTGCCAGTTGGCAGGTGGCGAAATCGCGCGCAGTTGCGTTTGCTGCCAGCCATGCCGCCAGAGCTGTTGGTACTGGTTTCTGCTGGCAAGACTGTTCATTAATGTTCGCTGGAAACCCGATAACGCAGTGACAATCATCACCATCA  >  NC_000913/2962420‑2962700
                                                                                                                                              |                                                                                                                                          
ccTTAACATAGCGGCTCCTGACTACTGACGATTCGGGCACTGCAGGCGGGTCATCTCGCCTTCTCTGCCCCCGGGTGAAACTAGCGTAACGCTGATGCTGGCACATCCCGCCTGCGATGTCTGCGTTCGGTTGACCTGCCCGt                                                                                                                                            >  2:25333/1‑143 (MQ=255)
         aGCGGCTCCTGCCTACTGACGATTCGGGCAATGCAGGCGGGTCATCTCGCCTTCTCTGCCCCCGGGTGAAACTAGCGTAACGCTGATGCTGACACATCCCGCCTGCGATGTCTGCATTCGGTTGACCTGCCAACTGGCAGGtc                                                                                                                                   <  2:189685/143‑2 (MQ=255)
            ggCTCCTGACTACTGACGATTCGGGCAATGCAGGCGGGTCATCTCGCCTTCTCTGCCCCCGGGTGAAACTAGCGTCACGCTGATGCTGACACATCCCGCCTGCGATGTCTGCGTTCGGTTCACCTGCCAGTTGGCAGGTAGCg                                                                                                                                >  1:229140/1‑143 (MQ=255)
            cgCTCCTGCCTACTGACGATTCGGGCAATGCAGGCGGGTCATCTCGCCTTCTCTGCCCCCGGGTGAAACTAGCGTAACGCTGATGCTGACACATCCCGCCTGCGATGTCTGCATTCGGTTGACCTGCCAACTGGCAGGtcaac                                                                                                                                <  1:511989/142‑5 (MQ=255)
                     ctactGACGATTCGGGCAATGCAGGCGGGTCATCTCGCCTTCTCTGCCCCCGGGTGAAACTAGCGTAACGCTGATGCTGACACATCCCGCCTGCGATGTCTGCATTCGGTTGACCTGCCAGTTGGCAGGTGGCGAAATcgcgc                                                                                                                       <  1:402941/143‑1 (MQ=255)
                            cGATTCGGGCAATGCAGGCGGGTCATCTCGCCTCCTCTGCCCCCGGGTGAAACTAGCGTAACGCTGATGCTGCCACATCCCGCCTGCGATGTCTGCATTCGGTTGACCTGCCAGTTGGCAGGTGGCGAAATCGCGCGCAGTTg                                                                                                                <  1:483890/143‑1 (MQ=255)
                                tCGGGCAATGCAGGCGGGTCATCTCGCCTTCTCTGCCCCCGGGTGAAACTAGCGTAACGCTGATGCTGACACATCCCGCCTGCGATGTCTGCATTCGGTTGACCTGCCAGTCGGCAGGTGGCGAAATCGCGCGCAGTTGCGtt                                                                                                            >  2:119207/1‑143 (MQ=255)
                                    gCAATGCAGGCGGGTCATCTCGCCTTCTCTGCCCCCGGGTGAAACTAGCGTAACGCTGATGCTGACACATCCCGCCTGCAATGTCTGCATTCGGTTGACCTGCCAGCTGGCAGGCGGCGACATCGCGCGCCGTTACgctatct                                                                                                        >  2:68120/1‑137 (MQ=255)
                                             gCGGGTCATCTCGCCTTCTCTGCCCCCGGGTGAAACTCGCGTACCGCTGCTGCTTACACCTCCCGCCTGCGCTCTCTGCATTCGGTTGACCTGCCAGTTAGCAGGGGCCGACATCGCCCGCAGTCGCGCTCGTTgccagccac                                                                                               >  2:496696/1‑142 (MQ=255)
                                                 gTCATCTCGCCCTCTCTGCCCCCGGGTGAAACTAGCGTAACGCTGATGCTGACACATCCCGCCTGCGATGTCTGCATTCGGTTGACCTGCCAGTTGGCAGGTGGCGAAATCGCGCGCAGTTGCGTTTGCTGCCAGCCATgccg                                                                                           <  2:309034/143‑1 (MQ=255)
                                                     tctcGCCTTCTCTGCCCCCGGGTGAAACTAGCGTAACGCTGATGCTGACACATCCCGCCTGCGATGTCTGCATTCGGTTGACCTGCCAGTTGGCAGGTGGCGAAATCGCGCGCAGTTGCGTTTGCTGCCAGCCATGCCGCCag                                                                                       >  1:556995/1‑143 (MQ=255)
                                                       tcGCCTTCTCTGCCCCCGGGTGAAACTAGCGTAACGCTGATGCTGACACATCCCGCCTGCGATGTCTGCATTCGGTTGACCTGCCAGTTGGCAGGTGGCGAAATCGCGCGCAGTTGCGTTTGCTTCCAGCCATGCCGCCagag                                                                                     >  1:571100/1‑143 (MQ=255)
                                                         gCCTTCTCTGCCCCCGGGTGAAACTAGCGTAACGCTGATGCTGACACATCCCGCCTGCGATGTCTGCATTCGGTTGACCTGCCAGTTGGCAGGTGGCGAAATCGCGCGCAGTTGCGTTTGCTGCCAGCCATGCCGCCAGAGCt                                                                                   <  1:559223/143‑1 (MQ=255)
                                                         cccTTCTCTGCCCCCGCGTCACACTAGCGTACCGCTGATGCTGACACATCCCGCCTGCGATGTCTGCATTCGGTTGACCTGCCAGTTGGCAGGTGGCGAAATCGCGCGCAGTTGCGTTTGCTGCCAGCCATGCCGCCAGAGCt                                                                                   <  1:98669/142‑1 (MQ=255)
                                                           gttCTCTGCCCCCGGGTGAAACTAGCGTAACGCTGAGGCTGACACATCCCGCCTGCGATGTCTGCATTCGGTTGACCTGCCAGTTGGCAGGTGGCGAAATCGCGCGCAGTTGCGTTTGCTGCCAGCCATGCCGCCAGAGCTGt                                                                                 <  1:481134/142‑1 (MQ=255)
                                                             tctcTGCCCCCGGGTGAAACTAGCGTAACGCTGATGCTGACACATCCCGCCTGCGATGTCTGCATTCGGTTGACCTGCCAGTTGGCAGGTGGCGAAATCGCGCGCAGTTGCGTTTGCTGCCAGCCATGCCGCCAGAGCTGTTg                                                                               >  2:301598/1‑143 (MQ=255)
                                                                    ccccGGGTGAAACTAGCGTAACGCTGATGCTGACACATCCCGCCTGCGATGTCTGCATTCGGTTGACCTGCCAGTTGGCAGGTGGCGAAATCGCGCGCAGTTGCGTTTGCTGCCAGCCCTGCCGCCAGAGCTGCTGGTACTgg                                                                        >  2:260870/1‑143 (MQ=255)
                                                                      ccGGGTGAAACTAGCGTAACGCTGATGCTGACACATCCCGCCTGCGATGTCTGCATTCGGTTGACCTGCCAGTTGGCAGGTGGCGAAATCGCGCGCAGTTGCGTTTGCTGCCAGCCATGCCGCCAGAGCTGGTGGTACTGGtg                                                                      >  2:568708/1‑142 (MQ=255)
                                                                             aaaCTAGCGTAACGCTGATGCTGACACATCCCGCCTGCGATGTCTGCATTCGGTTGACCTGCCAGTTGGCAGGTGGCGAAATCGCGCGCAGTTGCGTTTGCTGCCAGCCATGCCGCCAGAGCTGTTGGTACTGGTTTctgctg                                                               <  1:195317/143‑1 (MQ=255)
                                                                                cTAGCGTAACGCTGATGCTGACACATCCCGCCTGCGATGTCTGCATTCGGTTGACCTGCCAGTTGGCAGGTGGCGAAATCGCGCGCAGTTGCGTTTGCTGCCAGCCATGCCGCCAGAGCCGTTGGTACTGGTTTCTACCGGCa                                                            >  2:188396/1‑143 (MQ=255)
                                                                                     gTAACGCTGATGCTGACACATCCCGCCTGCGATGTCTGCATTCGGTTGACCTGCCAGTTGGCAGGTGGCGAAATCGCGCGCAGTTGCGTTTGCTCCCAGCCCTGCCGCCAGAGCCGTTGGTACTGATATCTGCTGGCaaaaat                                                       >  1:518836/1‑139 (MQ=255)
                                                                                           cTGATGCTGACACAGCCCGCCTGCGATGTCTGCATTCGGTTGACCTGCCAGTTGGCAGGTGGCGAAATCGCGCGCAGTTGCGTTTGCTGCCAGCCATGCCGCCAGAGCTGTTGGTACTGGTTTCTGCTGGCAAGACTGTTCAt                                                 <  1:449240/143‑1 (MQ=255)
                                                                                            tGATGCTGACACATCCCGCCTGCGATGTCTGCACAATTTTTACTTTTCATTTGGCAGGTGGCGAAATCGCGCGCAGTTGCGTTTGCTGCCAGCCATGCCGCCAGAGCTGTTGGTACTGGTTTCTGCTGGCCAGACTGTTCAtt                                                >  1:465557/1‑143 (MQ=255)
                                                                                             gATGCTGAAACATCCTGCCTGCGATGTCTGCATTCGGTTGACCTGCCAGTTGGCAGGTGGCGAAATCGCGCGCAGTTGCGTTTGCTGCCAGCCATGATACTAGAGCTGTTGGTACTGGTTTCTGCTGGCAAGACTGTTCATTa                                               <  2:302493/143‑1 (MQ=255)
                                                                                               ggCTGACACATCGCGCCTGCGAGTTCTGCATTCGGTTGACCTGCCAGTTGGCAGGTGGCGATATCGCGCGCAGTTGCGTTTGCTGCCAGCCATGCCGCCAGAGCTGTTGGTACTGGGTTCTGCTGGCAAGACTGTTCATTAAt                                             <  2:351784/142‑1 (MQ=255)
                                                                                                              ccTGCGATGTCTGCATTCGGTTGACCTGCCAGTTGGCAGGTGGCGAAATCGCGCGCAGTTGCGTTTGCTGCCAGCCATGCCGCCAGAGCTGTTGGTACTGGTTTCTGCTGGCAAGACTGTTCATTAATGTTCGCTGGAAAccc                              <  1:573347/143‑1 (MQ=255)
                                                                                                                     tGTCTGCATTCGGTTGACCTGCCAGTTGGCAGGTGGCGAAATCGCGCGCAGTTGCGTTTGCTGCCAGCCATGCCGCCAGAGCTGTTGGTACTGGTTTCTGCTGGCAAGACTGTTCATTAATGTTCGCTGGAAACCCGATAACg                       <  1:403852/143‑1 (MQ=255)
                                                                                                                     cgTCTGCATTCGGTTGACCTGCCAGTTGGCAGGTGGCGAAATCGCGCGCTGTTGCGTTTGCTGCCAGCCATGCCGCCAGAGCTGTTGGTACTGGTTTCTGCTGGCAAGACTGTTCATTAATGTTCGCTGGAAACCCGATAACg                       <  1:124626/142‑1 (MQ=255)
                                                                                                                              tCGGTTGACCTGCCAGTTGGCAGGTGGCGAAATCGCGCGCAGTCGCGTTTGCTGCCAGCCATGCCGCCAGAGCTGTTGGTACTGGTTTCTGCTGGCAAGACTGTTCATTAATGTTCGCTGGAAACCCGATAACGCAGTGACaa              <  1:25333/143‑1 (MQ=255)
                                                                                                                                    gACCTGCCAGTTGGCAGGTGGCGAAATCGCGCGCAGTTGCGTTTGCTGCCAGCCATGCCGCAAGAGCTACACGTACTGGTGTCTGCTGGCAAGACTCTGCATTAATGTTCGCCGGAAACCCGGGAACGCAGTGGCAatcctcg        >  2:203451/1‑142 (MQ=255)
                                                                                                                                    caCCTGCCAACTGGCAGGTGGCGAAATCGCGCGCAGTTGCGTTTGCTGCCAGCCATGCCGCCAGAGCTGTTGGTACTGGTTTCTGCTGGCAAGACTGTTCATTAATGTTCGCTGGAAACCCGATAACGCAGTGACAAtcatca        >  2:376501/2‑143 (MQ=255)
                                                                                                                                    caCCTGCCAACTGGCAGGTGGCGAAATCGCGCGCAGTTGCGTTTGCTGCCAGCCATGCCGCCAGAGCTGTTGGTACTGGTTTCTGCTGGCAAGACTGTTCATTAATGTTCGCTGGAAACCCGATAACGCAGTGACAAgcgtca        >  2:384488/2‑143 (MQ=255)
                                                                                                                                       cTGCCAGTTGGCAGGTGGCGAAATCGCGCGCAGTTGCGTTTGCTGCCAGCCATGCCGCCAGAGCTGTTGGTACTGGTTTCTGCTGGCAAGACTGTTCATTAATGTTCGCTGGAAACCCGATAACGCAGTGACAATCATCACCa     <  1:301598/143‑1 (MQ=255)
                                                                                                                                        tGCCAGTTGGCAGGTGGCGAAATCGCGCGCAGTTGCGTTTGCTGCCAGCCATGCCGCCAGAGCTGTTGGTACTGGTTTCTGCTGGCAAGACTGTTCATTAATGTTCGCTGGAAACCCGCTAACGCAGTGACAATCATCACCAt    >  1:325713/1‑143 (MQ=255)
                                                                                                                                          ccAGTTGGCAGGTGGCGAAATCGCGCGCAGTTGCGTTTGCTGCCAGCCATGCCGCCAGAGCTGTTGGTACTGGTTTCTGCTGGCAAGACTGTTCATTAATGTTCGCTGGAAACCCGATAACGCAGTGACAATCATCACCATCa  >  1:205599/1‑143 (MQ=255)
                                                                                                                                              |                                                                                                                                          
CCTTAACATAGCGGCTCCTGACTACTGACGATTCGGGCAATGCAGGCGGGTCATCTCGCCTTCTCTGCCCCCGGGTGAAACTAGCGTAACGCTGATGCTGACACATCCCGCCTGCGATGTCTGCATTCGGTTGACCTGCCAGTTGGCAGGTGGCGAAATCGCGCGCAGTTGCGTTTGCTGCCAGCCATGCCGCCAGAGCTGTTGGTACTGGTTTCTGCTGGCAAGACTGTTCATTAATGTTCGCTGGAAACCCGATAACGCAGTGACAATCATCACCATCA  >  NC_000913/2962420‑2962700

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑