| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_000913 | 2,971,159 | G→A | 12.2% | intergenic (+26/‑112) | ygdQ → / → ygdR | UPF0053 inner membrane protein YgdQ/DUF903 domain‑containing lipoprotein YgdR |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 2,971,159 | 0 | G | A | 12.2% | 97.8 / 6.8 | 49 | intergenic (+26/‑112) | ygdQ/ygdR | UPF0053 inner membrane protein YgdQ/DUF903 domain‑containing lipoprotein YgdR |
| Reads supporting (aligned to +/- strand): ref base G (20/23); new base A (4/2); total (24/25) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.17e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.86e-01 | |||||||||||
CTGGAAAGTTTCGACATCCACGTACCGAAAGGTTACATCTACTTCGCGATGTTCTTCTCTATTGCGGTTGAAAGCCTCAACTTGATTCGCAACAAAAAGAATCCGCTCTGATACTCCGTACGCTCTCCTGCGGGAGGGCGTACTCACTCCTTTTACCCTCACAGTTTAAGATTTTCCTGCTTTCAAGATATATAACGTCGGTTTATAAACAGACTATTATCATAGGTAGGCCAAATGAGGATAACCGGATGAAAAAATGGGCAGTAATAATTTCT > NC_000913/2971023‑2971297 | cgggAAAGGTGCGACATCCACGTACCGAAAGGTTACATCTACTTCGCGATGTTCTTCTCTATTGCGGTTGAAAGCCTCAACTTGATTCGCAACAAAAAGAATCCGCTCTGATACTCCGTACGCCCTCCCGCAGGAGAGCGTac < 1:119312/141‑1 (MQ=255) atggTTTCGAAATCCGCGGACCGAAAGGTTACATCTACTTCGCGATGTTCTTCTCTATTGCGGTTGAAAGCCTCAACCTGATTCGCAACAAAAAGAATCCGCTCTGATACTCCGTACGCTCTCCTGCGGGAGGGCGTactcac < 2:37912/140‑1 (MQ=255) aaaGTTTCGACATCCACGTACCGAAAGCTTACATCTACTTCGCGATGTTCTTCTCTATTGCGGTTGAAAGCCTCAACTTGATTCGCAACAAAAAGAATCCGCTCTGATACTCCGTACGCTCTCCTGCGGGAGGGCGTactcac < 1:509950/143‑1 (MQ=255) tCGACATCCACGTACCGAAAGGTTACATCTACTTCGCGATGTTCTTCTCTATTGCGGTTGAAAGCCTCAACTTGATTCGCAACAAAAAGAATCCGCTCTGATACTCCGTACGCTCTCCTGCGGGAGGGCGTACTCACTCCttt < 1:62609/143‑1 (MQ=255) aCGTACCGAAAGGTTACATCTACTTCGCGATGTTCTTCTCTATTGCGGTTGAAAGCCTCAACTTGATTCGCAACAAAAAGAATCCGCTCTGATACTCCGTACGCTCTCCTGCGGGAGGGCGTACTCACTCCTTTTACCCTcac > 1:377373/1‑143 (MQ=255) cGTACCGAAAGGTTACATTTACTTCGCGATGTTCTTCTCTATTGCGGTTGAAAGCCTCAACTTGATTCGCAACAAAAAGAATCCGCTCTGATACTCCGTACGCTCTCCTGCGGGAGGGCGTACTCACTCCTTTTACTCTcaca < 1:435229/143‑1 (MQ=255) aagggTTAGATCTACTGCGCGATGTTCTTCTCTACTGCGGTTGACAGCCTCAACTTGATTCGCAACAAAAAGAAGCCGCTCTGATACTCCGTACGCTCTCCTGCGGGAGGGCGTACTCACTCCTTTTACCCTCACAGTTTAAg < 2:503244/140‑1 (MQ=255) aaaGGTTACATCTACTTCGCGATGTTCTTCTCTATTGCGGTTGAAAGCCTCAACTTGATTCGCAACAAAAAGAATCCGCTCTGATACTCCGTACGCTCTCCTGCGGGAGGGCGTACTCACTCCTTTTACCCTCACAGTTTAAg > 1:161227/1‑143 (MQ=255) aaaGGTTACATCTACTTCGCGATGTTCTTCTCTATTGCGGTTGAAAGCCTCAACTTGATTCGCAACAAAAAGAATCCGCTCTGATACTCCGTACGCTCTCCTGCGGGAGGGCGTACTCACTCCTTTTACCCTCACAGTTTAAg > 2:379031/1‑143 (MQ=255) aaGGTTACATCTACTTCGCGATGTTCTTCTCTATTGCGGTTGAAAGCCTCAACTTGATTCGCAACAAAAAGAATCCGCTCTGATACTCCGTACGCTCTCCTGCGGGAGGGCGTACTCACTCCTTTTACCCTCACAGTTTAAGa < 1:588357/143‑1 (MQ=255) gTTACATCTACTTCGCGATGTTCTTCTCTATTGCGGTTGAAAGCCTCAACTTGATTCGCAACAAAAAGAATCCGCTCTGATACTCCGTACGCTCTCCTGCGGGAGGGCGTACTCACTCCTTTTACCCTCACAGTTTAAGAttt > 1:17102/1‑143 (MQ=255) gTTACATCTACTTCCTGATTTTCTTCGCTATTGCGGTGGAAAGCCTCAACTTGATTCGCAACAAAAAGAATCCGCTCTGCTACTCCGTACGCTCTCCTGCGGGAGGGCGTACTCGCTCCTTTTACCCTCACAGTTTAAGAttt < 2:372989/143‑1 (MQ=255) ttACATCTACTTCGCGATGTTCTTCTCTATTGCGGTTGAAAGCCTCAACTTGATTCGCAACAAAAAGAATCCGCTCTGATACTCCGTACGCTCTCCTGCGGGAGGGCGTACTCACTCCTTTTACCCTCACAGTTTAAGAtttt > 1:187249/1‑143 (MQ=255) aTCTACTTCGCGATGTTCTTCTCTATTGCGGTTGAAAGCCTCAACTTGATTCGCAACAAAAAGAATCCGCTCTGATACTCCGTACGCTCTCCTGCGGGAGGGCGTACTCACTCCTTTTACCCTCACAGTTTAAGATTTTCCTg < 1:444970/143‑1 (MQ=255) tCTACTTCGCGATGTTCTTCTCTATTGCGGTTGAAAGCCTCAACTTGATTCGCAACAAAAAGAATCCGCTCTGATAGTCCGTACGCTCTCCTGCGGGAGGGCGTACTCACTCCTTTTACCCTCACAGTTGAAGATCTTCCTGc > 2:649220/1‑143 (MQ=255) tCGCGATGTTCTTCTCTATTGCGGTTGAAAGCCTCAACTTGATTCGCAACAAAAAGAATCCGCTCTGATACTCCGTACGCTCTCCTGCGGGAGGGCGTACTCACTCCTTTTACCCTCACAGTTTAAGATTTTCCTGCTTTCaa < 1:160393/143‑1 (MQ=255) gATGTTATTCTCTATTGCGGTTGAAAGCCTCAACTTGATTCGCAACAAAAAGAATCCGCTCTGATACTCCGTACGCTCTCCTGCGGGAGGGCGTACTCAAAACATTTCTATTTATTGTTTAAGATTTTCCTGCTTTCAAGata < 1:316991/143‑1 (MQ=255) tcttctCTATTGCGGTTGAAAGCCTCAACTTGATTCGCAACAAAAAGAATCCGCTCTGATACTCCGTACGCTCTCCTGCGGGAGGGCGTACTCACTCCTTTTACCCTCACAGTTTAAGATTTTCCTGCTTTCAAGATATATaa < 2:365133/143‑1 (MQ=255) ttctCTATTGCGGTTGAAAGCCTCAACTTGATTCGCAACAAAAAGAATCCGCTCTGATACTCCGTACGCTCTCCTGCGGGAGGGCGTCCTCACTCCTTTTACCCTCACAGTTTAAGATTTTCCTGCTTTCAAGATATATAACg > 1:241384/1‑143 (MQ=255) ttctCTATTCCTGTTGCCGGCCTTAACTTGATTCGCAACTACATGCAGCCGCTCTGATACTCCGTACGCTCTCCTGCGGGAGGTCGTACTCCCTCCTTTTACCCTCACAGTTTAAGATTTTCCTGCTTTCAAGATATATAACg < 2:504775/143‑1 (MQ=255) ctctATTGCGGTTGAAAGCCTCAACTTGATTCGCAACAAAAAGAATCCGCTCTGATACTCCGTACGCTCTCCTGCGGGAGGGCGTACTCACTCCTTTTACCCTCACAGTTTAAGATTTTCCTGTTTTCAAGATATATAACGTc > 1:455888/1‑143 (MQ=255) ctctATTGCGGTTGAAAGCCTCAACTTGATTCGCAACAAAAAGAATCCGCTCTGATACTCCGTACGCTCTCCTGCGGGAGGGCGTACTCACTCCTTTTACCCTCACAGTTTAAGATCTTCCCGCTTTGAAGATATATACCGTc > 1:278162/1‑143 (MQ=255) tATTGCAGTGGAAAGCCTCAACGTGATTCGCATCGAAAATAATCCGCTCTGATACTCCGTACGCTCTCCTGCGGGAGGGCGTACTCACTCCTTTTACCCTCACAGTTTAAGATTTTCCTGCTTTCAAGATATATAACGTCGGt < 2:138372/143‑1 (MQ=255) gCGGTTGAAAGCCTCAACTTGATTCGCAACAAAAAGAATCCGCTCTGATACTCCGTACGCTCTCCTGCGGGAGGGCGTACTCACTCCTTTTCCCCTCACAGTTTAAGATTTTCCTGCTTTCAAGATCTATAACGTCGGTTtat > 2:103961/1‑143 (MQ=255) gTTGAAAGCCTCAACTTGATTCGCAACAAAAAGAATCCGCTCTGATACTCCGTACGCTCTCCTGCGGGAGGGCGTACTCACTCCTTTTACCCTCACAGTTTAAGCTTTTCCTGCTTTCAAGATATATAACGTCGGTATATaaa > 2:148589/1‑143 (MQ=255) gaaGCCTCAACTTTGTTCGGAACAAAAAGAATCCGCTCGGCTACTCCGTACGCTCTCCTGCGGGAGGGCTTACTCACTCCTTTTACCCTCACAGTTTAAGATTTTCCTGCTTTCAAGACATATAACGTCGGTTTATAAACAGa < 1:271483/142‑1 (MQ=255) aaGCCTCACCTTGATTCGCAACAAAAAGAATCCGCTCTGATACTCCGTACGCTCTCCTGCGGGAGGGCGTACTCACTCCTTTTACCCTCACAGTTTAAGATTTTCCTGCTTTCAAGATATATAACGTCGGTTTATAAACAGAc < 1:396337/143‑1 (MQ=255) aGCCTCAACTTGATTCGCAACAAAAAGAATCCGCTCTGATAATCCGTACGCTCTCCTGCGGGAGGGCGTACTCACTCCTTTTACCCTCACAGTTTAAGATTTTCCTGCTTTCAAGATATATAACGTCGGTTTATAAACAGACt < 2:168659/143‑1 (MQ=255) gCCTCAACTTGATTCGCAACAAAAACACTCCGCGCTGATACTCCGTACGCTCTCCTGCGGGAGGGCGTACTCACTCCTTTTACCCTCACAGTTTAAGATTTTCCTGCTTTCAAGATATATAACGTCGGTTTATAAACAGACta < 1:341522/143‑1 (MQ=255) ttGATTCTCACCATAACGAATCCGCTCTGATCCGCCGTACGCTCTCCTGCGGGAGGGCGTACTCACTCCTTTTACCCTCTCAGTTTAAGATTTTCCTGCTTTCAAGATATATAACGTCGGTTTATAAACAGACTATTATCAta < 2:184849/143‑1 (MQ=255) gtcacaaAGAGAATCCGCTCTGATTCTCCGTACGCTCTCCTGCGGGAGGGCGTACTCACTCCTTTTACCCTCACAGTTTAAGATTTTCCTGCTTTCAAGATATATACCGTCGGTTTATAAACAGACTATTATCATAGGTAGGc < 2:605080/140‑1 (MQ=255) caacaaAAAGAATCCGCTCTGATACTCCGTACGCTCTCCTGCGGGAGGGCGTCCTCACTCCTTTTACCCTCACCGTTTAAGATTTTCCTGCTTTCACGATATATAACGTCGGTTTATAAACAGACTATTATCATACGTAGGcc > 2:425310/1‑143 (MQ=255) caaAAAGAATCCGCTCTGATACTCCGTACGCTCTCCTGCGGGAGGGCGTACTCACTCCTTTTACCCTCACAGTTTAAGATTTTCCTGCTTTCAAGATATATAACGTCGGTTTATAAACAGACTATTATCATAAGTAGGCCaaa > 2:187857/1‑143 (MQ=255) aaaaaGAATCCGCTCTGATACTCCGTACGCTCTCCTGCGGGAGGGCGTACTCACTCCTTTTACCCTCACAGTTTAAGATTTTCCTGCTTTCAAGATATATAACGTCGGTTTATAAACAGACTATTATCATAGGTAGGCCaagt > 1:51841/1‑141 (MQ=255) tCCGCTCTGATACTCCGTACGCTCTCCTGCGGGAGGGCGTACTCACTCCTTTTACCCTCACAGTTTAAGATTTTCCTGCTTTCAAGATATATACCGTCCGTTTATAAACAGACTATTATCATAGGTAGGCCAAATGAGGATaa > 1:222047/1‑143 (MQ=255) ctGATACTCCGTACGCTCTCCTGCCGGAGGGCGTACTCACTCCTTTTACCCTCACAGTTTAAGATTTTCCTGCTTTCAAGATATATAACGTCGGTTTATAAACAGACTATTATCATAGGTAGGCCAAATGAGGATAACCGGAt < 2:439741/143‑1 (MQ=255) ctGACACTCCGTACGCTCTCCCGCGGGAGAGCGTACTCACTCCTTTTACCCTCACAGTTTAAGATTTTCCTGCTTTCAAGATATATAACGTCGGTTTATACACAGACTATTATCATAGGTAGGCCAAATGAGGATAACCGGAt < 1:137521/143‑1 (MQ=255) tGATACTCCGTACGCTCTCCTGCGGGAGGGCGTTCACACGCCTGTTGCCTTTACCGTTTAAGATGTTCCTGCTTTCAAGGTAGATAACGTCGGTTTATAAACAGACTATTATCAGAGCTCGGCCAGATGAGGATAACCGGacg > 2:449206/1‑141 (MQ=255) gttACTCCGTGCGCTGTGCTGCGGGAGGTCGTAGTCACTCCTTTTACCCTCACAGTTTAAGATTTTCCTGCTTTCAAGAGATATAACGTCTGTTTATAAACAGACTATTATCATAGGTAGGCCAAATGAGGATAACCGGATGa < 2:5378/141‑1 (MQ=255) gATACTCCGTACGCTCTCCTGCGGGAGGGCGTACTCACTCCTTTTACCCTCACAGTTTAAGATTTTCCTGCTTTCAAGATATATAACGTCGGTTTATAAACAGACTATTATCATAGGTAGGCCAAATGAGGATAACCGGATGa < 1:379031/143‑1 (MQ=255) aTACTCCGTACGCTCTCCTGCGGGAGGGCGTACTCACTCCTTTTACCCTCACAGTTTAAGATTTTCCTGCTTTCAAGATATATCACGTCGGTTTATAAACAGACTATTATCATAGGTAGGCCAAATGAGGATAACCGGAGGaa > 1:9888/1‑143 (MQ=255) cTCCGTACGCTCTCCTGCGGGAGGGCGTACTCACTCCTTTTACCCTCACAGTTTAAGATTTTCCTGCTTTCAAGATATATAACGTCGGTTTATAAACAGACTATTATCATAGGTAGGCCAAATGAGGATAACCGGATGaaaaa > 2:11985/1‑143 (MQ=255) agTACGCCCTCCCGCAGGAGAGCGTACTCACTCCTTTTACCCTCACAGTTTAAGATTTTCCTGCTTTCAAGATATATAACGTCGGTTTATAAACAGACTATTATCATAGGTAGGCCAAATGAGGATAACCGGATGAAAAAATg > 2:295016/2‑143 (MQ=255) gTACGCTCTCCTGCGGGAGGGCGTACTCACTCCTTTTACCCTCACAGTGTAAGATTTTCCTGCTTTCAAGATATATAACGTCGGTTTATAAACAGACTATTATCATAGGTAGGCCAAATGAGAATAACAGGAGGAAGAAATgg > 2:19444/1‑143 (MQ=255) tACGCCCTCCCGCAGGAGAGCGTACTCACTCCTTTTACCCTCACAGTTTAAGATTTTCCTGCTTTCAGGATATATAACGTCGGTTTATAAACAGACTATTATCATAGGTAGGCCAAATGAGGATAACCGGATGAAAAAATggg > 1:502304/1‑143 (MQ=255) tACGCCCTCCCGCAGGAGAGCGTACTCACTCCTTTTACCCTCACAGTTTAAGATTTTCCTGCTTTCAAGATATGTAACGTCGGTTTATAAACAGACTGTTATCATAGGTAGCACAAGTGAGGCTAACCGGAAGAACAAATggg > 1:243929/1‑143 (MQ=255) tACGCCCTCCCGCAGGAGAGCGTACTCACTCCTTTTACCCTCACAGTTTAAGATTTTCCTGCTTTCAAGATATATAACGTCGGTTTATAAACAGACTATTATCATAGGTAGGACAAATGAGGATAACCGGATGAAAAAATggg > 1:32766/1‑143 (MQ=255) gCTCTCCTGCGGGAGGGCGTACTCACTCCTTTTACCCTCACAGGTTAAGATTTTCCTGCTTTCAAGATATATAACGTCGGTTTATAAACAGACTATTATCATAGGTAGGCCAAATGAGGATAACCGGATGAAAAAATGGGCAg > 1:83924/1‑143 (MQ=255) ggAGGGCGTACTCACTCCTTTTACCCTCACAGTTTAAGATTTTCCTGCTTTCAAGATATATAACGTCGGTTTATAAACAGACTATTATCATAGGTAGGCCAAATGAGGATAACCGGATGAAAAAATGGGCAGTAATAATTTCt < 2:187249/143‑1 (MQ=255) | CTGGAAAGTTTCGACATCCACGTACCGAAAGGTTACATCTACTTCGCGATGTTCTTCTCTATTGCGGTTGAAAGCCTCAACTTGATTCGCAACAAAAAGAATCCGCTCTGATACTCCGTACGCTCTCCTGCGGGAGGGCGTACTCACTCCTTTTACCCTCACAGTTTAAGATTTTCCTGCTTTCAAGATATATAACGTCGGTTTATAAACAGACTATTATCATAGGTAGGCCAAATGAGGATAACCGGATGAAAAAATGGGCAGTAATAATTTCT > NC_000913/2971023‑2971297 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |