BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	3,041,398	T→G	100%	L29R (CTT→CGT)	ygfZ →	folate‑binding protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,041,398	0	T	G	100.0%	91.5 / NA	35	L29R (CTT→CGT)	ygfZ	folate‑binding protein
Reads supporting (aligned to +/- strand): ref base T (0/0); new base G (15/20); total (15/20)
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

ATAGCTCTTTTACCATTAGTCATTAATACGCCGTTAAGCAACTCAGGACTCTATTATGGCTTTTACACCTTTTCCTCCCCGTCAGCCTACGGCTTCTGCCCGTTTACCGCTGACGCTGATGACGCTTGATGACTGGGCGCTTGCCACCATTACTGGCGCGGACAGCGAAAAATATATGCAGGGTCAGGTGACAGCAGATGTCAGCCAGATGGCAGAAGATCAGCACCTGCTCGCCGCCCATTGCGACGCCAAAGGTAAAATGTGGAGCAATT  >  NC_000913/3041258‑3041529

aTAGCTCTTTTACCATTAGTCATTAATACGCCGTTAAGCAACTCAGGACTCTATTCTGGCTTTTACACCTTTTCCTCCCCGTCAGCCTACGGCTTCTGCCCGTTTACCGCTGACGCTGATGACGCTTGATGACTGGGCGCGTg                                                                                                                                   <  2:99834/143‑1 (MQ=255)

 tccacattttACCATTAGTCATTAATACGCCGTTAAGCAACTCAGGACTCTATTATGGCTTTTACACCTTTTCCTCCCCGTCAGCCTACGGCTTCTGCCCGTTTACCGCTGCCCCTGATGCCGCGTGATCACTGGGCGCGTGc                                                                                                                                  >  2:513192/7‑143 (MQ=255)

         ttACCATTAGTCATTAATACGCCGTTAAGCAACTCAGGACTCTATTATGGCTTTTACACCTTTTCCTCCCCGTCAGCCTACGGCTTCTGCCCGTTTACCGCTGACGCTGATGACGCTTGATGACTGGGCGCGTGCCACCATTa                                                                                                                          <  2:32770/143‑1 (MQ=255)

              aTTAGTCATTAATACGCCGTTAAGCAACTCAGGACTCTATGATGGCTTTTACACCTTTTCCTCCCCGTCAGCCTCCGGCTTCTGCCCGTTTACCGCTGACGCTGATGACGCTTGATGACTGGGCGCGTGCCACCATTACTGgc                                                                                                                     <  1:529275/143‑1 (MQ=255)

                   tCATTAATACGCCGTTAAGCAACTCAGGACTCTATTATGGCTTTTACACCTTTTCCTCCCCGTCAGCCTACGGCTTCTGCCCGTTTACCGCTGACGCTGATGACGCTTGATGACTGGGCGCGTGGCACCATTACTGGcgcgca                                                                                                                >  1:468163/1‑141 (MQ=255)

                   tCATTAATACGCCGTTAAGCAACTCAGGACTCTATTATGGCTTTTACACCTTTTCCTCCCCGTCAGCCTACGGCTTCTGCCCGTTTACCGCTGACGCCGATGACGCTTGATGACTGGGCGCGTCCCACCAGTACTGGCCCggc                                                                                                                >  2:602347/1‑142 (MQ=255)

                     aTTAATACGCCGTTAAGCAACTCAGGACTCTATTATGGCTTTTACACCTTTTCCTCCCCGTCAGCCTACGGCTTCTGCCCGTTTACCGCTGACGCTGATGCCGCCTGATGCCTGGGCGCGTGCCACCATTACTGGCGCGGACa                                                                                                              >  1:619153/1‑143 (MQ=255)

                     aTTAATACGCCGTTAAGCAACTCAGGACTCTATTATGGCTTTTACACCTTTTCCTCCCCGTCAGCCTACGGCTTCTGCCCGTTTACCGCTGACGCTGATGACGCTTGATGACTGGGCGCGTGCCACCATTACTGGCGCGGCCa                                                                                                              >  1:507733/1‑143 (MQ=255)

                          tACGCCGTTAAGCAACTCAGGACTCTATTATGGCTTTTACACCTTTTCCTCCCCGTCAGCCTACGGCTTCTGCCCGTTTACCGCTGACGCTGATGACGCTTGATGACTGGGCGCGTGCCACCATTACTGGCGCGGACAGCgca                                                                                                         >  1:498868/1‑141 (MQ=255)

                                 ttAAGCAACTCAGGACTTGATTATGGCTTTTACACCTTTTCCTCCCCGTCAGCCTACGGCTTCTGCCCGTTTACCGCTGACGCTGATGCCGCTTGATGACTGGGCGCGTGCCTCCATTACTGGCGCGGACAGCGAAAAatata                                                                                                  <  1:43802/143‑1 (MQ=255)

                                      cAACTCAGGACTCTATTATGGCTTTTACACCTTTTCCTCCCCGTCAGCCTACGGCTTCTGCCCGTTTACCGCTGACGCTGATGACGCTTGATGACTGGGCGCGTGCCACCATTACTGGCGCGGACAGCGGAAAGTATATGCAg                                                                                             >  1:498165/1‑143 (MQ=255)

                                            aGGACTCTATTAGGGCTTTGACCCCTTTTCCTCCCCGTCAGCCTCCGGCGTCTGCCCGGCTGCCGCTGACGCTGATGACGCTTGATGACTGGGCGCGTGCCACCATTACTGGCGCGGACAGCGAAAAATATATGCAGGGTCAg                                                                                       <  2:45172/143‑1 (MQ=255)

                                                 tctATTATGGCTTTTACACCTTTTCCTCCCCGTCAGCCTCCGGCTTCTGCCCGTTTACCGCCACCGCTCATGACGCTTGATGACTGGGCGCGTGCCACCATTCCTGGCGCGGACAGCGAACACTATATGcaggtgcaggtgcc                                                                                  >  1:118732/1‑141 (MQ=255)

                                                     ttaggtcTTTTACACCGTTTCCTCCCCGTCAGCCTACGGCTTCTGCCCGTCTTCCTCCATCGCTGATGACGCTTGATGACTGGGCGCGTGCCACCATTACTGGCGCGGACAGCGAAAAATATATGCAGGGTCAGGTGAcagca                                                                              <  1:493403/137‑1 (MQ=255)

                                                      tatGGCTTTTACTCCTTTTCCTCCCCGTCAGCCTACGGCTTCTGCCCGTTTACCGCTGACGCTGATGACGCTTGATGACTGGGCGCGTGCCACCATTACTGGCGCGGACAGCGAAAAATATATGCAGGGTCAGGTGAcagcag                                                                             <  1:16265/143‑1 (MQ=255)

                                                      tacggCTTTGACACCGTTTCCTCCCCGTCAGCCTACGGCTTCTGCCCGTTTACCGCTGACGCTGATGACGCTTGATGACTGGGCGCGTGCCACCATTACTGGCGCGGACAGCGAAAAATATATGCAGGGTCAGGTGAcagcag                                                                             <  1:18659/140‑1 (MQ=255)

                                                        tGGCTTTTACACCTTTTCCTCCCCGTCAGCCTACGGCTTCTGCCCGTTTACCGCTGACGCTGATGACGCTTGATGACTGGGCGCGTGCCACCATTACTGGCGCGGACAGCGAAAAATATATGCAGGGTCAGGTGAcagcagct                                                                           >  1:514865/1‑141 (MQ=255)

                                                                acacCTTTTCCTCCCCGTCAGCCTACGGCTTCTGCCCGTTTACCGCTGACGCTGATGACGCTTGATGACTGGGCGCGTGCCACCATTACTGGCGCGGACAGCGAAAAATATATGCAGGGTCAGGTGACAGCAGCTGTCAGCCa                                                                   >  1:569717/1‑143 (MQ=255)

                                                                    cTTTTCCTACCCGTCAGCCTACGGCTTCTGCCCGTTTACCGCTGACGCTGATGACGCTTGCTGACTGGGCGCGTGCCACCATTACTGCGGCGGACAGCGAAAAATATATGAAGGGTCAGGTGACCGCAGATGTCCACCAGATg                                                               >  2:38232/1‑143 (MQ=255)

                                                                      tttCCTCCCCGTCAGCCGACGGCTTCTGCCCGTTTACCGCTGACGCTGATGACGCTTGATGACTGGGCGCGTGCCACCATTACTGGCGCGGACAGCGAAAAATATCTGCAGCGTCAGGTGACAGCAGATGTCAGCCAGATGGc                                                             <  2:241106/143‑1 (MQ=255)

                                                                          cgccccGTCAGCCGGCGGCTTCGGCCGGTTTACCGCTGACGCTGATGACGCTTGATGACTGGGCGCGTGCCACCATTACTGGCGCGGACAGCGAAAAAGATATGCAGGGTCAGGTGACAGCAGATGTCAGCCAGATGGCagaa                                                         <  2:126612/141‑1 (MQ=255)

                                                                           tccCCGTCAGCCTACGGCTTCTGCCCGTTTACCGCTGACGCTGATGACGCTTGATGACTGGGCGCGTGCCACCATTACTGGCGCGGACAGCGAAAAATATATGCAGGGTCAGGTGACAGCAGATGTCAGCCAGATGGCagaag                                                        >  2:641412/1‑143 (MQ=255)

                                                                           tccCCGTCAGCCTACGGCTTCTGCCCGTTTACCGCTGACGCTGATGACGCTTGATGACTGGGCGCGTGCCACCATTACTGGCGCGGACAGCGAAAAATATATGCAGGGCCAGGTGACAGCAGATGTCAGCCAGATGGCagaag                                                        >  2:658349/1‑143 (MQ=255)

                                                                             ctcGTCAGCCTACGGCTTCTTCCCGTTTACCGCTGACAATGATGACGCGTGATGACTGGGCGCGTGCCACCATTACTGGCGCGGACAGCGAAAAATATATGCAGGGTCAGGTGACAGCAGATGTCAGCCAGATGGCAGAAGAt                                                      <  1:382061/141‑1 (MQ=255)

                                                                              ccGTCGTCCTACGGCTTCTGTCCGTTTACCGCTGACGCTGATGACGCTTGATGACTGGGCGCGTGCCACCAGTACTGGCGCGGACAGCGAAAAATATATGCAGGGTCAGGTGACAGCAGATGTCAGCCAGATGGCAGAAGATc                                                     <  2:2510/143‑1 (MQ=255)

                                                                               cGTCAGCCTACGGCTTCTGCCCGTTTACCGCTGACGCTGATGACGCTTGATGACTGGGCGCGTGACACCATTACTGGCGCGGACAGCGAAAAATATATGCAGGGTCAGGTGACAGCAGATGTCAGCCAGATGGCAGAAGATCa                                                    <  1:214440/143‑1 (MQ=255)

                                                                                       taggtcTTCGGCCCGGTTACGGCTGACGCTGAGGACGCTTGATGACTGGGCGCGTGCCACCATTACTGGCGCCGACAGCGAAAAATATATGCAGGGTCAGGTGACAGCAGATGTCAGCCAGATGGCAGAAGATCAGCACCTGc                                            <  2:316544/138‑1 (MQ=255)

                                                                                                tGCCCGTTTACCGCTGACGCTGATGACGCTTGATTACTGGGCGCGTTCCACCATTACTGGCGCGGACAGCGAAAAATATATGCAGGGTCAGGTGACAGCAGATGTCAGCCAGATGGCAGAAGATCAGCACCTGCTCGCCGccc                                   <  1:201365/143‑1 (MQ=255)

                                                                                                 gCCCGTTTACCGCTGACGCTGATGACGCTTGATGACTGGGCGCGTGCCACCATTACTGGCGCGGACAGCGAAAAATATATGCAGGGTCAGGTGACAGCAGATGTCAGCCAGATGGCAGAAGATCAGCACCTGCTCGCCGCCCa                                  >  1:643407/1‑143 (MQ=255)

                                                                                                             ctgacgctgaTGACGCTTGATGACTGGGCGCGTGCCACCATTACTGGCGCGGACAGCGAAAAATATATGCAGGGTCAGGTGACAGCAGATGTCAGCCAGATGGCAGAAGATCAGCACCTGCTCGCCGCCCATTGCGACGCCaa                      <  2:547152/143‑1 (MQ=255)

                                                                                                                  ggtgacgaCGCTCAATGACTGTGCGCGTTCCACCATTACGGGCGCGGACAGCGAAAAATATAGGCAGGGTCAGGTGACAGCAGATGTCAGCGAGATGGCAGAAGATCAGCACCTGCTCGCCGCCCATTGCGACGCCAAAGGTa                 <  1:526305/141‑1 (MQ=255)

                                                                                                                  gcgtatgaCGCTTGACGACTGGGCGCGTGCCACCATTACTGGCGCGGACAGCGAAAAATATATGCAGGGTCAGGTGACAGCAGATGTCAGCCAGATGGCAGAAGATCAGCACCTGCTCGCCGCCCATTGCGACGCCAAAGGTa                 <  1:358370/139‑1 (MQ=255)

                                                                                                                         aCGCTTGATGACTGGGCGCGTGCCACCATTACTGGCGCGGACAGCGAAAAATATATGCAGTGTCAGGTGACAGCAGATGTCAGCCAGATGGCAGAAGATCAGCACCTGCTCGCCGCCCATTGCGACACCAAAGGTAAAAtgtg          <  2:587082/143‑1 (MQ=255)

                                                                                                                           gCTTGATGACTGGGCGCGTGCCACCATTACTGGCGCGGACAGCGAAAAATATATGCAGGGTCAGGTGACAGCAGATGTCAGCCAGATGGCAGAAGATCAGCACCTGCTCGCCGCCCATTGCGATGCCAAAGGTAAAATGTGGa        <  1:513192/143‑1 (MQ=255)

                                                                                                                                 tgaCTGGGCGCGTGCCACCATTACTGGCGCGGACAGCGAAAAATATATGCAGGGTCAGGTGACAGCAGATGTCAGCCAGATGGCAGAAGATCAGCACCTGCTCGCCGCCCATTGCGACGCCAAAGGTAAAATGTGGAGCAAtt  >  2:398143/1‑143 (MQ=255)

ATAGCTCTTTTACCATTAGTCATTAATACGCCGTTAAGCAACTCAGGACTCTATTATGGCTTTTACACCTTTTCCTCCCCGTCAGCCTACGGCTTCTGCCCGTTTACCGCTGACGCTGATGACGCTTGATGACTGGGCGCTTGCCACCATTACTGGCGCGGACAGCGAAAAATATATGCAGGGTCAGGTGACAGCAGATGTCAGCCAGATGGCAGAAGATCAGCACCTGCTCGCCGCCCATTGCGACGCCAAAGGTAAAATGTGGAGCAATT  >  NC_000913/3041258‑3041529

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 8 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`