| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_000913 | 3,210,838 | A→G | 100% | K20E (AAG→GAG) | rpsU → | 30S ribosomal subunit protein S21 |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 3,210,838 | 0 | A | G | 100.0% | 61.8 / NA | 21 | K20E (AAG→GAG) | rpsU | 30S ribosomal subunit protein S21 |
| Reads supporting (aligned to +/- strand): ref base A (0/0); new base G (8/13); total (8/13) | |||||||||||
| Rejected as polymorphism: Frequency below/above cutoff threshold. | |||||||||||
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. | |||||||||||
AGTAAAATTCCGCACCATTTTGAAATAAGCTGGCGTTGATGCCAGCGGCAAACCGAATTAATCAAAGGTGAGAGGCACATGCCGGTAATTAAAGTACGTGAAAACGAGCCGTTCGACGTAGCTCTGCGTCGCTTCAAGCGTTCCTGCGAAAAAGCAGGTGTTCTGGCGGAAGTTCGTCGTCGTGAGTTCTATGAAAAACCGACTACCGAACGTAAGCGCGCTAAAGCTTCTGCAGTGAAACGTCACGCGAAGAAACTGGCTCGCGAAAACGCA > NC_000913/3210703‑3210975 | aGTAAAATTCCGCACCATTTTGAAATAAGCTGGCGTTGATGCCAGCGGCAAACCGAATTAATCAAAGGTGAGAGGCACATGCCGGTAATTAAAGTACGTGAAAACGAGCCGTTCGACGTAGCTCGGCGTCGCTTCGAGCGGTc > 1:353251/1‑143 (MQ=255) cGCACCATCTTGAAATAAGCTGGCGTTGATGCCAGCGGCAAACCGAATTAATCAAAGGTGAGAGGCACATGCCGGTAATTAAAGTACGTGAAAACGAGCCGTTCGACGTAGCTCTGCGTCGCTTCGAGCGTTCCTGCGaaaaa < 1:224689/143‑1 (MQ=255) cGCACAATTTTTAAACCGGCGGGGGGTGATGCCAGCGGTATCCCGAAGTAAGCAAGGGTGAGAGGCACCAGCCGGTAATTATAGTACGTGAAAACGAGCCGTTCGACGTAGCTCTGCGTCGCTTCGAGCGTTCCTGCGaaaaa < 2:223572/143‑1 (MQ=255) aTTGTGAAATAAGCTGGCGTTGATGCCAGCGGCAAACCGAATTAATCAAAGGTGAGAGGCACATGCCGGTAATTAAAGTACGTGAAAACGAGCCGTTCGACGTAGCTCTGCGTCGCTTCGAGCGTTCCTGCGAAAAAGCAGgt < 1:273927/143‑1 (MQ=255) tAAGCTGGCGTTGATGCCAGCGGCAAACCGAATTAATCAAAGGTGAGAGGCACATGCCGGTAATTAAAGTCCGTGAAAACGAGCCATTCGACGTAGCTCTGCGTCGCTACGAGGGTTACTGCGACAAAGCAGGTGTCCTTGCg > 2:655354/1‑143 (MQ=255) ttGATGCCATGGGCAAACCGAATTAATCAAAGGTGAGAGGCACATAGCAGTGATTTGTGTGTGTGAAAACGAGCCGTTCGACGTAGCTCTGCGTCGCTTCGAGCGTTCCTGCGAAAAAGCAGGTGTTCTGGCGGAAGTtcgtc < 2:659349/143‑1 (MQ=255) aTGCCAGCGGCAAACCGAATTAATCAAAGGTGAGAGGCACATGCCGGTAATTAAAGTACGTGAAAACGAGCCGTTCGACGTAGCTCTGCGTCGCTTCGAGCGTTCCTGCGAAAAAGCAGGTGTTCTGGCGGAAGTtcgtcgtc < 1:446977/143‑1 (MQ=255) ccAGCGGCAAACCGAATTAATCAAAGGTGAGAGGCACATGCCGGTAATTAAAGTACGTGAAAACGAGCCGTTCGACGTAGCTCTGCGTCGCTTCGAGCGTTCCTGCGAAAAAGCGGGTGTTCTGGCGGAAGTTCGTCGTCGTg > 1:511125/1‑143 (MQ=255) cacaCCGAATTAATCAAAGGTGAGAGGCACATGCCGGTAATTAAAGTACGTGAAAACGAGCCGTTCGACGTAGCTCTGCGTCGCTTCGAGCGTTCCTGCGAAAAAGCAGGTGTTCTGGCGGAAGTTCGTCGTCGTGAGTTCTa < 1:383261/140‑1 (MQ=255) cAAACCGAATTAATCAAAGGTGAGAGGCACATGCCGGTAATTAAAGTACGTGAAAACGAGCCGTTCGACGTAGCTCTGCGTCGCTTCGAGCGTTCCTGCGAAAAAGCAGGTGTTCTGGCGGAAGTTCGTCGTCGTGAGTTCTa < 1:379751/143‑1 (MQ=255) aaccAAAGGTGAGAGGCACAGGCCGGTAATTAAAGTACGTGAAAACGAGCCGTTCGACGTAGCTCTGCGTCGCTGCGAGCGTTCCTGCGAAAAAGCAGGTGTTCTGGCGGAAGTTCGTCGTCGTGAGTTCTATGAAAAACCGa < 1:514182/140‑1 (MQ=255) agGCACATGGCGGTAATTAAAGTACGTGAAAACGAGCCGTTCGACGTAGCTCTGCGTCGCTTCGAGCGTTCCTGCGAAAAAGCAGGTGTTCTGGCGGAAGTTCGTCGTCGTGAGTTCTATGAAAAACCGACTACCGAACGTaa < 1:174380/143‑1 (MQ=255) agGCACATGCCGGTAATTAAAGTACGTGAAAACGAGCCGTTCGACGTAGCTCTGCGTCGCTTCGAGCGTTCCTGCGAAAAAGCAGGTGTTCTGGCGGAAGTTCGTCGTCGTGAGTTCTATGAAAAACCGACTACCGAACGTaa < 1:578679/143‑1 (MQ=255) cGGTAATTAAAGTACGTGAAAACGAGCCGTTCGACGTAGCTCTGCGTCGCTTCGAGCGTTCCTGCGAAAAAGCAGGTGTCCTCTCTGAAGTTCGTCGTCGTGAGTTCTATGAAAAACCGACTACCGAACGTAAGCGCGCTaaa < 1:427262/143‑1 (MQ=255) aaTTAAAGTACGTGAAAACGAGCCGTTCGACGTAGCTCTGCGTCGCTTCGAGCGTTCCTGCGAAAAAGCAGGTGTTCTGGCGGAAGTTCGTCGTCGTGAGTTCTATGAAAACCCGACTACCGAACGTAAGCGCGCTAAAGCtt < 1:13474/143‑1 (MQ=255) gTGAAAACGAGCCGTTCGACGTAGCTCTGCGTCGCTTCGAGCGTTCCTGCGAAAAAGCAGGTGTTCTGGCGGAAGTTCGTCGTCGTGAGTTCTATGAAAAACCGACTACCGAACGTAAGGGCGCTAAAGCTTATGCAGTGaaa > 1:157390/1‑143 (MQ=255) cGTTCGACGTAGCTCTGCGTCGCTTCGAGCGTTCCTGCGAAAAAGCAGGTGTTCTGGCGGAAGTTCGTCGTCGTGAGTTCTATGAAAAACCGACTACCGAACGTAAGCGCGCTAAAGCTTCTGCAGTGCAACGTCACGCgaag > 2:305842/1‑143 (MQ=255) cGTTCGACGTAGCTCTGCGTCGCTTCGAGCGTTCCTGCGAAAAAGCAGGTGTGCTGGCGGAAGTTCGTCGTCGTGAGTTCTATGAAAAACCGACTACCGAACGTACGCGCGCTAAAGCTTCTGCAGTGAAAAGTCACGCgaag > 2:9344/1‑143 (MQ=255) gCTCTGCGTCGCTTCGAGCGTTCCTGCGAAAAAGCAGGTGTTCTGGCGGAAGTTCGTCGTCGTGAGTTCTATGAAAAACCGACTACCGAACGTAAGCGCGCTAAAGCTTCTGCAGTGAAACGTCACGCGAAGAAACTGGCTcg < 1:602678/143‑1 (MQ=255) gTCGCTTCGAGCGTTCCTGCGAAAAAGCAGGTGTTCTGGCGGAAGTTAGTCGTCGCGAGTTCTATGAATACCCGACTACCGAACGTAAGCGCGCTAAAGCTTCGGCAGTGAAACGTCACGCGAAGAGACTGGATCGCGaaacc > 2:662388/1‑141 (MQ=255) gCTTCGAGCGTTCCTGCGAAAAAGCAGGCGTTCTGGCGGACGTTCGTCGTCGTGAGTTCTATGAAAAACCGACTACCGAACGTAAGCGCGCTAAAGCGTCTGCAGTGAAACGCCACCCGAAGAGACTGGGTGGCGGAGacgcc > 2:141474/1‑142 (MQ=255) | AGTAAAATTCCGCACCATTTTGAAATAAGCTGGCGTTGATGCCAGCGGCAAACCGAATTAATCAAAGGTGAGAGGCACATGCCGGTAATTAAAGTACGTGAAAACGAGCCGTTCGACGTAGCTCTGCGTCGCTTCAAGCGTTCCTGCGAAAAAGCAGGTGTTCTGGCGGAAGTTCGTCGTCGTGAGTTCTATGAAAAACCGACTACCGAACGTAAGCGCGCTAAAGCTTCTGCAGTGAAACGTCACGCGAAGAAACTGGCTCGCGAAAACGCA > NC_000913/3210703‑3210975 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |