| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_000913 | 835,195 | A→G | 10.5% | K709R (AAA→AGA) | dinG → | ATP‑dependent DNA helicase DinG |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 835,195 | 0 | A | G | 10.5% | 103.1 / 2.4 | 46 | K709R (AAA→AGA) | dinG | ATP‑dependent DNA helicase DinG |
| Reads supporting (aligned to +/- strand): ref base A (24/17); new base G (3/2); total (27/19) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.83e-01 | |||||||||||
CTGGGGCGAAGTGGTTATCTACGACAAACGCTTGCTGACCAAAAATTATGGCAAGCGACTACTGGATGCATTACCGGTATTTCCGATAGAGCAACCGGAAGTCCCTGAAGGTATAGTTAAAAAGAAAGAAAAAACGAAATCCCCACGCCGTCGGCGGCGTTAATGATGTGAGTCAGGTAAGGAGTCGTAAATGGACTATCGCAAAATCATTAAAGAGATCGGGCGCGGGAAAAACCACGCGCGCGATTTAGACCGGGATACTGCGCGCGGTCTGTA > NC_000913/835058‑835333 | ctcgtgCGAAGTAGTTATCTACGAAAAACGCCTGCTGACCAAAAATTATGGCAAGCGACTACTGGATGCATTACCGGTATATCCGATAGAGCAACCGGAAGTCCCTGAAGGTATAGTTAAAAAGAAAGAAAAAACGAAATccc < 2:604585/138‑1 (MQ=255) tGGGGCGAAGTGGTTATCTACGACAAACGCTTGCTGACCAAAAATTATGGCAAGCGACTACTGGATGCATTACCGGTATTTCCGATAGAGCAACCGGAAGTCCCTGCAGGTATAGTTAAAAAGAAAGAAAAAACGAAATcccc < 1:438039/143‑1 (MQ=255) aaGTGGTTATCTACGACAAACGCTTGCTGACCAAAAATTATGGCAAGCGACTACTGGATGCATTACCGGTATTTCCGATAGAGCAACCGGAAGTCCCTGCAGGTATAGTTAAAAAGAAAGAAAAAACGAAATCCCCACGCCGt > 2:529210/1‑143 (MQ=255) tGGTTATCTACGACAAACGCTTGCTGACCAAAAATTATGGCAAGCGACTACTGGATGCATTACCGGTATTTCCGATAGAGCAACCGGAAGTCCCTGAAGGTATAGTTAAAAAGAAAGAAAAAACGAACTCCCCACGCCGTcgg > 2:182556/1‑143 (MQ=255) gTTATCTACGACAAACGCTTGCTGACCAAAAATTATGGCAAGCGACTACTGGATGCATTACCGGTATTTCCGATAGCGCAACCGGAAGTCCCTGAAGGTATAGTTAAAAAGAAAGAAAAAACGAAACCCCCACGCCGTcggcg > 2:96689/1‑143 (MQ=255) gTTATCTACGACAAACGCTTGCTGACCAAAAATTATGGCAAGCGACTACTGGATGCATTACCGGTATTTCCGATAGAGCAACCGGAAGTCCCTGAAGGTAAGATCAACAGGAAAGAAAAAACGAAATCCCCACGCCGTcggcg > 1:459017/1‑143 (MQ=255) gTTATCTACGACAAACGCTTGCTGACCAAAAATTATGGCAAGCGACTACTGGATGCATTACCGGTATTTCCGATAGAGCAACCGGAAGTCCCTGAAGGCATAGTTAAAAAGAAAGAAAAAACGAAATCCCCACGCCGTcggcg > 2:214041/1‑143 (MQ=255) gTTATCTACGACAAACGCTTGCTGACCAAAAATTATGGCAAGCGACTACTGGATGCATTACCGGTATTTCCGATAGAGCAACCGGAAGTCCCTGAAGGAGTAGTGAAAACAGAAGAAAAAACGAAATCACCACGCCGTcggcg > 2:5894/1‑143 (MQ=255) gTTATCTACGACAAACGCTTGCTGACCAAAAATGATGGCAAGCGACTACTGGATGCATTACCGGTATTTCCGATAGAGCAACCGGAAGTCCCTGAAGGGAGAGTTAAAAAGAGAGAAAAAACGAGATCCCCACGCCATcggcg > 2:336151/1‑143 (MQ=255) tATCTACGACAAACGCTTGCTGACCAAAAATTATGGCAAGCGACTACTGGATGCATTACCGGTATTTCCGATAGAGCAACCGGAAGTCCCTGAAGGTATAGTTAAAAAGAAAGAAAAAACGAAATCCCCACGCCGTCggcggc > 2:336933/1‑143 (MQ=255) aCGACAAAAGCTAGCTGACCAAAAAGTATGGCAAGCGACTACTGGATGCATTACCGGTATTTCCGATAGAGCAACCGGAAGTCCCTGAAGGTATAGTTAAAAAGAAAGAAAAAACGAAATCCCCACGCCGCCGACGGCGtggg < 1:282569/143‑4 (MQ=255) cGACAAACGCTTGCTGACCAAAAATTATGGCAAGCGACTACTGGATGCATTACCGGTATTTCCGATAGAGCAACCGGAAGTCCCTGAAGGTATAGTTAAAAAGAAAGCAAAAACGAAATCCCCACGCCGTCGGCGGCGTTaaa > 2:342540/1‑142 (MQ=255) aCAAACGCTTGCTGAGCAAATATTGTAGAAAGCGACTACTGGCTGCATTACCGGTATTTCCGATAGAGCAACCGGAAGTCCCTGAAGGTAGAGTTAAAAAGAAAGAAAAAACGAAATCCCCACGCCGTCGGCGGCGTTAatga < 2:460808/143‑1 (MQ=255) aCAAACGCTTGCTGACCAAAAATTATGGCAAGCGACTACTGGATGCATTACCGGTATTTCCGATAGAGCAACCGGAAGTCCCTGAAGGTATAGTTAAAAAGAAAGAAAAAACGAGATCCCCACGCCGTCGGCGGCGTTAatga > 2:446785/1‑143 (MQ=255) aCAAACGCTTGCTGACCAAAAATTATGGCAAGCGACTACTGGATGCATAACCGGTATTTCCGATAGAGCAACCGGAAGTCCCTGAAGGTATAGTTAAAAAGAAAGAAACAACGAGATCCCCACGCCGTCGGCGGCGTTAatga > 2:442522/1‑143 (MQ=255) cAAACGCTTGCTGACCAAAAATTATGGCAAGCGACTACTGGATGCATTACCGGTATTTCCGATAGAGCAACCGGAAGTCCCTGAAGGTATAGTTAAAAAGAAAGAAAAAACGAAATCCCCACGCCGTCGGCGGCGTTAatgat < 1:609021/143‑1 (MQ=255) cGCTTGCTGACCAAAAATTATGGCAAGCGACTACTGGATGCATTACCGGTATTTCCGATAGATCAACCGGAAGTCCCTGAAGGTATAGTTAAAAAGAAAGAAAAAACGAAATCCCCACGCCGTCGGCGGCGTTAATGATGTGa < 1:596923/143‑1 (MQ=255) gCTGACCAAAAATTATGGCAAGCGACTACTGGATGCATTACCGGTATTTCCGATAGAGCAACCGGAAGTCCCTGAAGGTATAGTTAAAAAGAAAGAAAAAACGAAATCCCCACGCCGTCGGCGGCGTTAATGATGTGAGTCAg < 2:665124/143‑1 (MQ=255) cgaaCCAAAAATTATGGCCAGCGACTACTGGATGCATTACCGGTATTTCCGATAAAGCAACCGGAAGTCACTGAAGGTATAGTTAAAAAGAAAGAAAAAACGAAATCCCCACGCCGTCGGCGGCGTTAATGATGTGAGTCAgg < 2:494667/140‑1 (MQ=255) tGACCAAAAATTATGGCAAGCGACTACTGGATGCATTACCGGTATTTCCGATAGAGCAACCGGAAGTCCCTGAAGGTATAGTTAAAAAGAAAGAGAAAACGAAATCCCCACGCCGTCGGCGGCGTTAATGATGTGAggcgggg > 1:431381/1‑137 (MQ=255) cAAAAATTATGGCAAGCGACTACTGGATGCATTACCGGTATTTCCGATAGAGCAACCGGAAGTCCCTGAAGGTATAGTTAAAAAGAAAGCAAAAACGAAATCCCCACGCCGTCGGCGGCGTTGATGATGTGAGTCAGGTAAgg > 2:181459/1‑143 (MQ=255) aaaaaTTATGGCAAGCGACTACTGGATGCATTACCGGTATTTCCGATAGAGCAACCGGAAGTCCGTGAAGGTATAGTTAAAAAGAAAGAAAAAACGAAATCCCCACGCCGTCGGCGGCGCTAATGATGTGCGTCAGGTAAGGa > 1:96468/1‑143 (MQ=255) aaaaaTTATGGCAAGCGACTACTGGATGCATTACCGGTATTTCCGATAGACAATCAACTTGGGCTGTAAGGTATAGTTAAAAAGAAAGAAAAAACGAAATCCCCACGCCGTCGGCGGCGTTAATGATGTGAGTCAGGTAAGGa < 1:154694/143‑1 (MQ=255) aaaaaTTATGGCAAGCGAATACAGAATGCATTACCGGTATTTCCGATAGAGCAACCGGAAGTCCCTGAAGGTATAGTTAAAAAGAAAGAAAAAACGAAATCCCCACGCCGTCGGCGGCGTTAATGATGTGAGTCAGGTAAGGa < 1:91818/143‑1 (MQ=255) aTTATGGCAAGCGACTACTGGATGCATTACCGGTATTTCCGATAGAGCAACCGGAAGTCCCTGAAGGTATAGTTAAAAAGAAAGAAAAAACGAGATCCCCACGCCGTCGGCGGCGTTAATGATGTGAGTCAGGTAAGGAGTCg < 1:446785/143‑1 (MQ=255) aTTATGGCAAGCGACTACTGGATGCATAACCGGTATTTCCGATAGAGCAACCGGAAGTCCCTGAAGGTATAGTTAAAAAGAAAGAAAAAACGAGATCCCCACGCCGTCGGCGGCGTTAATGATGTGAGTCAGGTAAGGAGTCg < 1:442522/143‑1 (MQ=255) aggCAAGTGGCTACTGGATGCATTACCGGTATTTCCGATAGACCAACCGGAAGTCCCTGAAGGTATAGTTAAAAGGAAAGAAAAAACGAAATCCCCAGGCCGTCGGCGGCGTTAATGATGTGAGTCAGGTAAGGAGTCGTaaa < 1:542002/142‑1 (MQ=255) gCAAGCGACTACTGGATGCATTACCGGTATTTCCGATAGAGCAACCGGAAGTCCCTGAAGGTATAGTTAAAAAGAAAGAAAAAACGAAATCCCCACGCCGTCGGCGGCATTAATGATGTGAGTCAGGTAAGGAGTCGTAAATg > 1:526387/1‑143 (MQ=255) ctGGATGCATTACCGGTATTTCCGATAGAGCAACCGGAAGTCCCTGAAGGTATAGTTAAAAAGAAAGAAAAAACGAAATCCCCACGCCGTCGGCGGCGTTAATGATGTGAGTCAGGAAAGGCGTCGTAAATGGACTATCGCac > 2:191578/1‑142 (MQ=255) gATGCATTACCGGTATTTCCGATAGAGCAACCGGAAGTCCCTGAAGGTATAGTTAAAAAGAAAGAAAAAACGAAATCCCCACGCCGTCGGCGGCGTTAATGATGTGAGTCAGGTAAGGAGTCGTAAATGGACTATCGCAAAAt > 1:116899/1‑143 (MQ=255) ggCATTAACGGGATTGCCGAGAGAGGAACGGGAAGGCGCTGAAGGTATAGTTAAAAAGAAAGAAAAAACGAAATCCCCACGCCGTCGGCGGCGTTAATGATGTGAGTCAGGTAAGGAGTCGTAAATGGACTATCGCAAAATCa < 2:27892/142‑1 (MQ=255) gTATTTCCGATAGAGCAACCGGAAGTCCCTGAAGGTATAGTTAAAAAGAAAGAAAAAACGAAATCCCCACGCCGTCGGCGGCGTTAATGATGTGAGTCAGGTAAGGAGTCGTAAATGCACTATCGCAAAATCATGAAAgagag > 2:328739/1‑142 (MQ=255) cGATAGAGCAACCGGAAGGCCCTGAAGGTAGAGTTAAAAAGAAAGAAAAAACGAAATCCCCACGCCGTCGGCGGCGTTAATGATGTGAGTCAGGTAAGGAGTCGTAAATGGACTAACGCAAGATCATTAAAGAGATCGGgcgc > 1:293571/1‑143 (MQ=255) gATAGAGCAACCGGAAGTCCCTGAAGGCATCATTAAAAAGATTGGAAAACCGAAATCCCCACGCCGTCGGCGGCGTTAATGATGTGAGTCAGGTAAGGAGTCGTAAATGGCCTATCGCACAATCATTAAAGAGATCGGGcgcg > 1:645661/1‑143 (MQ=255) ggCAACCGGAAGTCCCTGAAGGTATAGTTAAAAAGAAAGGAAGAACGAAAGCCTCACGCCGTCGGCGGCGGTAATGATGTGAGTCAGGTAAGGAGTCGTAAATGGACTATCGCAAAATCATTAAAGAGATCGGGCGCGGGaaa < 2:467720/142‑1 (MQ=255) cAACCGGAAGTCCCTGAAGGTATAGTTAAAAAGAAAGAAAAAACGAAATCCCCACGCCGTCGGCGGCGTTAATGATGTGAGTCAGGTAAGGAGTCGTAACTGGACTATCGCAAAATCATTAAAGCGATCGGGCGCGGGagaaa > 1:140642/1‑143 (MQ=255) aaCCGGAAGTCCCTGAAGGTATAGTTAAAAAGAAAGAAAAAACGAAATCCCCACGCCGTCGGCGGCGTTAATGATGTGAGTCAGGTAAGGAGTCGTAAATGGACTATCGCAAAATCATTAAAGAGATCGGGCGCGGGAAAAAc < 1:336933/143‑1 (MQ=255) aaCCGGAAGTCCCTGAAGGTATAGTTAAAAAGAAAGAAAAAACGAAATCCCCACGCCGTCGGCGGCGTTAAGGATGTGAGTCAGGTAAGGAGTCGTACATGGACTCTCGCAAAATCATTAAAGAGATCGGGCGCGGGAAAAAc > 2:253668/1‑143 (MQ=255) gAAGTCCCTGAAGGTATAGTTAACAAGAAAGAACAAAAGGAACGACCACACCGTCGGCGGCGTTAATAATGTGAAGCAGGTAAGGAAGCGGAAATGGACTATCGGAAAATCAGTGAAGAGAGCGGGCGCGCGAACAACCAcgc > 2:662755/1‑143 (MQ=255) tGAAGGTATAGTTAAAAAGAAAGAAAAAACGAAATCCCCACGCCGTCGGCGGCGTTAATGATGTGAGTCAGGTAAGGAGTCGTAAATGGCCTATCGCAAAATCGTTAACGAGAGCGGGCGGGGGAAGACCcgcgcgcgtgctt > 1:637889/1‑138 (MQ=255) ataGTTAAAAAGAAAGAAAAAACGAAATCCCCACGCCGTCGGCGGCGTTAATGATGTGAGGCAGGTAAGGAGTCGTAAATGGACTATCGCAAAATCATTAAAGAGATCGGGCGCGGGAAAAACCACGCGCGCGATTTAGACCg < 1:305017/143‑1 (MQ=255) ttAAAAAGAAAGAAAAAACGAAATCCCCACGCCGTCGGCGGCGTTAATGATGTGAGTCAGGTAAGGAGTCGTAAATGGACTATCGCAAAATCATTAAAGAGATCGGGCGCGGGAAAAACCACGCGCGCGATTTAGACAGGGAt > 2:236640/1‑143 (MQ=255) agaacAAGCGAAATCCCCACGCCGTCGGCGGCGTTAATGATGTGAGTCAGGTAAGGAGTCGTAAATGGACTATCGCAAAATCATTAAAGAGCTCGGGCGCGGGAAAAACCACGCGCGCGATTTAGACCGGGATACTGcgcgcg < 1:256143/143‑1 (MQ=255) aaaaaCGAAATCCCCACGCCGTCGGCGGCGTTAATGATGTGAGTCAGGTAAGGAGTCGTAAATGGACTATCGCAAAATCATTAAAGAGATCGGGCGCGGGAAAAACCACGCGCGCGATTTAGACCGGGATACTGCGCGCGGTc < 1:354297/143‑1 (MQ=255) aaaCGAAATCCCCACGCCGTCGGCGGCGTTAATGATGTGAGTCAGGTAAGGAGTCGTAAATGGACTATCGCAAAATCATTAAAGAGATCGGGCGCGGGAAAAACCACGCGCGCGATTTAGACCGGGATACTGCGCGCGGTCTg > 1:666377/1‑143 (MQ=255) aCGAAATCCCCACGCCGTCGGCGGCGTTAATGATGTGAGTCAGGTAAGGAGTCGTAAATGGACTATCGCAAAATCATTAATGAGATCGGGCGTGGGAAAAACCACGCGCGCGATTTAGACCGGGATACTGCGCGCGGTCTGta > 1:342667/1‑143 (MQ=255) | CTGGGGCGAAGTGGTTATCTACGACAAACGCTTGCTGACCAAAAATTATGGCAAGCGACTACTGGATGCATTACCGGTATTTCCGATAGAGCAACCGGAAGTCCCTGAAGGTATAGTTAAAAAGAAAGAAAAAACGAAATCCCCACGCCGTCGGCGGCGTTAATGATGTGAGTCAGGTAAGGAGTCGTAAATGGACTATCGCAAAATCATTAAAGAGATCGGGCGCGGGAAAAACCACGCGCGCGATTTAGACCGGGATACTGCGCGCGGTCTGTA > NC_000913/835058‑835333 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |