| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_000913 | 125,085 | 2 bp→CA | 100% | coding (2069‑2070/2664 nt) | aceE → | pyruvate dehydrogenase E1 component |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 125,085 | 0 | T | C | 100.0% | 131.2 / NA | 43 | I690T (ATC→ACA) | aceE | pyruvate dehydrogenase E1 component |
| Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (20/23); total (20/23) | |||||||||||
| Rejected as polymorphism: Frequency below/above cutoff threshold. | |||||||||||
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. | |||||||||||
| * | NC_000913 | 125,086 | 0 | C | A | 100.0% | 133.0 / NA | 43 | I690T (ATC→ACA) | aceE | pyruvate dehydrogenase E1 component |
| Reads supporting (aligned to +/- strand): ref base C (0/0); new base A (20/23); total (20/23) | |||||||||||
| Rejected as polymorphism: Frequency below/above cutoff threshold. | |||||||||||
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. | |||||||||||
TCACAGCCACATTCAGTCGCTGACTATCCCGAACTGTATCTCTTACGACCCGGCTTACGCTTACGAAGTTGCTGTCATCATGCATGACGGTCTGGAGCGTATGTACGGTGAAAAACAAGAGAACGTTTACTACTACATCACTACGCTGAACGAAAACTACCACATGCCGGCAATGCCGGAAGGTGCTGAGGAAGGTATCCGTAAAGGTATCTACAAACTCGAAACTATTGAAGGTAGCAAAGGTAAAGTTCAGCTGCTCGGCTCCGGTTCTAT > NC_000913/124948‑125220 || tCACAGCCTCATTCAGTTGCTGACAATCCGCAACTGTACCTCGCACGACACGGCTTACGCTTACGAAGTTGCGGACAGCATGCACGACGGTCTGGAGCGTATGTCCGGTGAAAAACAAGAGAACGTTTACTACTACACAACTa < 1:479254/143‑1 (MQ=255) aGCCACATTCAGTCGCTGACTATCCCGAACTGTCTCTCTTACGACCCGGCTTACGCTTACGAAGTTGCTGTCATCATGCATGACGGTCTGGCGCGTATGTACGGTGAAAAACAAGAGAACGTTTACTACTACACAACTACGCt < 1:475373/143‑1 (MQ=255) cacaTTCAGTCGCTGACTATCCCGAACTGTATCTCTTACGACCCGGCTTACGCTTACGAAGTTGCTGTCATCATGCATGACGGTCCGGAGCGTATGTACGGTGAAAAACAAGAGAACGTTAACTACTACACAACTACGCTGaa > 2:509904/1‑143 (MQ=255) acaTTCAGTCGCTGACTATCCCGAACTGTATCTCTTACGACCCGGCTTACGCTTACGAAGTTGCTGTCATCATGCATGACGGTCTGGAGCGTATGTACGGTGAAAAACAAGAGAACGTTTACTACTACACAACTACGCTGAAc < 2:354554/143‑1 (MQ=255) caTTCAGTCGCTGACTATCCCGAACTGTATCTCTTACGACCCGGCTTACGCTTACGAAGTTGCTGTCATCATGCATGACGGTCTGGAGCGTATGTACGGTGAAAAACAAGAGAACGTTTACTACTACACAACTCCGCTGCACg > 1:246658/1‑143 (MQ=255) aGTCGCTGACTATCCCGAACTGTATCTCTTACGACCCGGCTTACGCTTACGAAGTTGCTGTCATCATGCATGACGGTCTGGAGCGTATGTACGGTGAAAAACAAGAGAACGTTTACTACTACACAACTACGCTGAACGAAAAc > 1:10745/1‑143 (MQ=255) ggCTGACTAGCCCGAACTGTGTCTCTTACGACCCGGCTTACGCTTACGAAGTTGCTGTCATCATGCATGACGGTCTGGAGCGTATGTACGGTGAAAAACAAGAGAACGTTTACTACTACACAACTACGCTGAACGAAAACTAc < 2:514038/142‑1 (MQ=255) tATCCCGAACTGTATCTCTTACGACCCGGCTTACGCTTACGAAGTTGCTGTCATCATGCATGACGGTCTGGAGCGTATGTACGGTGAAAAACAAGAGAACGTTTACTACTACACAACTACGCTGAACGAAAACTACCACATGc > 2:648430/1‑143 (MQ=255) cTGTATCTCTTACGACCCGGCTTACGCTTACGAAGTTGCTGTCATCATGCATGACGGTCTGGAGCGTATGTACGGTGAAAAACAAGAGAACGTTTACTACTACACAACTACGCTGAACGAAAACTACCACATGCCGGCAATGc < 1:214217/143‑1 (MQ=255) tctTACGACCCGGCTTACGCTTACGAAGTTGCTGTCATCATGCATGACGGTCTGGAGCGTATGTACGGTGAAAAACAAGAGAACGTTTACTACTACACAACTACGCTGAACGAAAACTACCACATCCCGGCAATGCCGGAAgg > 1:168987/1‑143 (MQ=255) ttACGACCCGGCTTACGCTTACGAAGTTGCTGTCATCATGCATGACGGTCTGGAGCGTATGTACGGTGAAAAACAAGAGAACGTTTACTACTACACAACTACGCTGAACGAAAACTACCACATGCCGGCAATGCCGGAAGGTg > 1:556069/1‑143 (MQ=255) aCGTCCCGGGTTACGCTTACGAAGTTGCTGTCATCATGCATGACGGTCTGGAGCGTATGTACGGTGAAAAACAAGAGAACGTTTACTACTACACAACTACGCTGAACGAAAACTACCACATGCCGGCAATGCCGGAAGGTGCt < 1:101282/143‑1 (MQ=255) cGACCCGGCTTACGCTTACGAAGTTGCTGTCATCATGCATGACGGTCTGGAGCGTATGTACGGTGAAAAACAAGAGAACGTTTACTACTACACAACTACGCTGAACGAAAACTACCACATGCCGGCAATGCCGGAAGGTGCTg < 2:62456/143‑1 (MQ=255) cGGGTTACGCTTACGAAGTTGCTGTCATCATGCATGACGGTCTGGAGCGTATGTACGGTGAAAAACAAGAGAACGTTTACTACTACACAACTACGCTGAACGAAAACTACCACATGCCGGCAATGCCGGAAGGTGCTGAGGaa < 2:314099/143‑1 (MQ=255) ggCTTACGCCTACGAAGTTGCTGTCATCATGCATGACGGTCTGGAGCGTATGTACGGTGAAAAACAAGAGAACGTTTACTACTACACAACTACGCTGAACGAAAACTACCACATGCCGGCAATGCCGGAAGGTGCTGAGGAAg < 1:628013/143‑1 (MQ=255) tgacggttacgAAGTTGCTGTCATCATGCATGACGGTCTGGAGCGTATGTACGGTGAAAAACAAGAGAACGTTTACTACTACACAACTACGCTGAACGAAAACTACCACATGCCGGCAATGCCGGAAGGTGCTGAGGAAGGTa < 2:375396/141‑1 (MQ=255) acgAAGTTGCTGTCATCATGCATGACGGTCTGGAGCGTATGTACGGGGAAAAACAAGAGAACGTTTACTACTACACAACTACGCTGAACGAAAACTACCACATGCCGGCAATGCCGGAAGGTGCTGAGGAAGGTATCCGTaaa < 1:413647/143‑1 (MQ=255) cgAAGTTGCTGTCATCATGCATGACGGTCTGGAGCGTATGTACGGTGAAAAACAAGAGAACGTTTACTACTACACAACTACGCTGAACGAAAACTACCACATGCCGGCAATGCCGGAAGGTGCTGAGGAAGGTATCCGTAAAg < 2:371711/143‑1 (MQ=255) aaGTCGCTGTCACCATGCATGACGGTCTGGAGCGTATGTACGGTGAAAAACAAGAGAACGTTTACTACTACACAACTCCGCTGAACGAAAACTACCACATGCCGGCAATGCCGGAAGGTGCTGAGGAAGGTATCCGTAAAGGt < 1:368359/143‑1 (MQ=255) gTTGCTGTCATCATGCATGACGGTCTGGAGCGTATGTACGGTGAAAAACAAGAGAACGTTTACTACTACACAACTACGCTGAACGAAAACTACCACATGCCGGCAATGCCGGAAGGTGCTGAGGAAGGTATCCGTAAAGGTAt < 1:249888/143‑1 (MQ=255) tGTCATCATGCATGACGGTCTGGAGCGTATGTACGGTGAAAAACAAGAGAACGTTTACTACTACACAACTACGCTGAACGAAAACTACCACATGCCGGCAATGCCGGACGGTGCTGAGGAAGGTATCCGCACAGGCATCTACa > 2:433790/1‑143 (MQ=255) tcatcaTGTATGACGGTCTGGAGCGTGTGTACGGTGAAAAACAAGAGAACGTTTACTACTACACAACTACGCTGAACGAACACTACCACATGCCGGCAATGCCGGAAGGTGCTGAGGAAGGTATCCGTAAAGGTATCTACaaa < 2:522296/143‑1 (MQ=255) atcatGCATGACGGTCTGGAGCGTATGTACGGTGAAAAACAAGAGAACGTTTACTACTACACAACTACGCTGAACGAAAACTACCACATGCCGGCAATGCCGGAAGGTGCTGAGGAAGGTATCCGTAAAGGTATCTACAAACt > 1:651994/1‑143 (MQ=255) tcatGCATGACGGTCTGGAGCGTATGTACGGTGAAAACCAAGAGAACGTTTACTACTACACAACTACGCTGAACGAAAACTACCACATGCCGGCAATGCCGGAAGGTGCTGAGGAAGGTATCCGTAAAGGTATCTACAAACTc > 1:285745/1‑143 (MQ=255) gcatgACGGTCTGGAGCGTATGTACGTTGAAAAACAAGAGAACGTTTACTACTACACAACTACGCTGAACGAAAACTACCACATGCCGGCAATGCCGGAAGGTGCTGAGGAAGGTATCCGTAAAGGTATCTACAAACTCGaaa < 2:529723/143‑1 (MQ=255) acatgACGTTTTGTAGCGTATGTACGGTGAAAAAAAAGATAATTTTTACTACTACACAACTACGCTGAACGAAAACTACCACATGCCGGCAATGCCGGAAGGTGCTGAGGAAGGTATCCGTAAAGGTATCTACAAACTCGaaa < 2:460243/142‑1 (MQ=255) tgACGGTCTGGAGCGTATGTACGGTGAAAAACAAGAGAACGTTTACTACTACACAACTACGCTGAACGAAAACTACCACATGCCGGCAATGCCGGAAGGTGCTGAGGAAGGTATCCGTAAAGGTATCTACAAACTCGAAACTa < 2:493962/143‑1 (MQ=255) aCGGTCTGGAGCGTATGTACGGTGAAAAACAAGAGAACGTTTACTACTACACAACTACGCTGAACGAAAACTACCACATGCCGGCAATGCCGGAAGGTGCTGAGGAAGGTATCCGTAAAGGTATCTACAAACTCGAAACTAtt > 2:300831/1‑143 (MQ=255) ggTCTGGAGCGTATGTACGGTGAAAAACAAGAGAACGTTTACTACTACACAACTACGCTGAACGAAAACTACCACATGCCGGCAATGCCGGAAGGTGCTGAGGAAGGTATCCGTAAAGGTATCTACAAACTCGAAACTATTGa > 1:325109/1‑143 (MQ=255) ggAGCGTATGTACGGTGAAAAAGAAGAGAGAGTGATCTACTACACAACTACGCTGAACGAAAACTACCCCATGCCAATAATCCCGGAAGGTGCTGAAGAAGGTATACGTAAAGGTATCGACAAACTAGAAACAATGGAAGGTa > 2:367551/1‑143 (MQ=255) tACGGTGAAAAACAAGAGAACGTTTACTACTACACAACTACGCTGAACGAAAACTACCACATGCCGGCAATGCCGGAAGGTGCTGAGGAAGGTATCCGTAAAGGTATCTACAAACTCGAAACTATTGAAGGTAGCAAAGTTaa > 1:172570/1‑143 (MQ=255) aCGGTGAAAAACAACAGAACCTTTACTACTACACAACTACGCTGAACGAAAACTACCCCATGCCGGCAATGCCGGAAGGTGCTGAGGAAGGTATCCGTAAAGGTATCTACAAACTCGAAACTATTGAAGGTAGCAAAGGTaaa < 1:562720/143‑1 (MQ=255) cgggcaaaaaCAAGAGAACGTTTACTACGACACAACTACGCTGAACGAAAACTACCACATGCCGGCAATGCCGGAAGGTGCTGAGGAAGGTATCCGTAAAGGTATCTACAAACTCGAAACTATTGAAGGTAGCAAAGGTAAAg < 2:611083/138‑1 (MQ=255) cGGTGAAAAACAAGAGAACGTTTACTACTACACAACTACGCTGAACGAAAACTACCACATGCCGGCAATGCCGGAAGGTGCTGAGGAAGGTATCCGTAAAGGTATCTACAAACTCGAAACTATTGAAGGTGGCACAGGTAAAg > 1:489287/1‑143 (MQ=255) gTGAAAAACAAGAGAACGTTTACTACTACACAACTACGCTGAACGAAAACTACCACATGCCGGCAATGCCGGAAGGTGCTGAGGAAGGTATGCGTAAAGGTATCTACAAACTCGAAACTATTGAAGGTAGCACCGGTAGAGtt > 2:580811/1‑143 (MQ=255) gAAAAACAAGAGAACGTTTACTACTACACAACTACGCTGAACGAAAACTACCACATGCCGGCAATGCCGGAAGGTGCTGAGGAAGGTATCCGTAAAGGTATCTACAAAATCGAAACTATTGAAGGTAGCAAAGGTAAAGTTCa > 1:69224/1‑143 (MQ=255) cAAGAGAACGTTTACTACTACACAACTACGCTGAACGAAAACTACCACAGGCCGGCAATGCCGGAAGGTGCTGAGGAAGGTATCCGTAAAGGTATCTACAAACTCGAAACTATTGAAGGTAGCAAAGGTAAAGTTCAgctgct < 1:412977/143‑1 (MQ=255) aaCGTTTACTACTACACAACTACGCTGAACGAAAACTACCACATGCCGGCAATGCCGGAAGGTGCTGAGGAAGGTATCCGTAAAGGTATCTACAAACTCGAAACTATTGAAGGTAGCAAAGGTAAAGTTCAGCTGCGCGGCTc > 1:555865/1‑143 (MQ=255) gTTTACTACTACACAACTACGCTGAACGAAAACTACCACATGCCGGCAATGCCGGAAGGTGCTGAGGAAGGTATCCGTAAAGGTATCTACAAACTCGAAACTATTGAAGGTAGCAAAGGTAAAGTTCAGCTGCTCGGCTCCgg < 2:651994/143‑1 (MQ=255) tttACTACTACACAACTACGCTGAACGAAAACTACCACATGCCGGCAATGCCGGAAGGTGCTGAGGAAGGTATCCGTAAAGGTATCTACAAACTCGAAACTATTGAAGGTAGCAAAGGTAAAGTTCAGCTGCTCGGCTCCGGt > 1:609868/1‑143 (MQ=255) ttACTACTACACAACTGCGCTGAACGAAAACTACCACATGCCGGCAATGCCGGAAGGTGCTGAGGAAGGTATCCGTAAAGGTATCTACAAACTCGAAACTATTGAAGGTAGCAAAGGTAAAGTTCAGCTGCTCGGCTCCGGtt < 2:10745/143‑1 (MQ=255) tactacACAACTACGCTGAACGAAAACTACCACATGCCGGCAATGCCGGAAGGTGCTGAGGAAGGTATCCGTAAAGGTATCTACAAACTCGAAACTATTGAAGGTAGCAAAGGTAAAGTTCAGCTGCTCGGCTCCGGTTCTAt > 1:93666/1‑143 (MQ=255) tactacACAACTACGCTGAACGAAAACTACCACATGCCGGCAATGCCGGAAGGTGCTGAGGAAGGTATCCGTAAAGGTATCTACAAACTCGAAACTAATGAAGGCAGCAACAATGAGTGTCAGCTGCTCGGCTCCGGGTCTAt > 1:123232/1‑143 (MQ=255) || TCACAGCCACATTCAGTCGCTGACTATCCCGAACTGTATCTCTTACGACCCGGCTTACGCTTACGAAGTTGCTGTCATCATGCATGACGGTCTGGAGCGTATGTACGGTGAAAAACAAGAGAACGTTTACTACTACATCACTACGCTGAACGAAAACTACCACATGCCGGCAATGCCGGAAGGTGCTGAGGAAGGTATCCGTAAAGGTATCTACAAACTCGAAACTATTGAAGGTAGCAAAGGTAAAGTTCAGCTGCTCGGCTCCGGTTCTAT > NC_000913/124948‑125220 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |