Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	123,050	A→T	I12F (ATC→TTC)	aceE →	pyruvate dehydrogenase E1 component

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	123,050	0	A	T	100.0%	174.7 / NA	59	I12F (ATC→TTC)	aceE	pyruvate dehydrogenase E1 component
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base T (25/34);  total (25/34)

AAGCGCGGCAACTAAACGTAGAACCTGTCTTATTGAGCTTTCCGGCGAGAGTTCAATGGGACAGGTTCCAGAAAACTCAACGTTATTAGATAGATAAGGAATAACCCATGTCAGAACGTTTCCCAAATGACGTGGATCCGATCGAAACTCGCGACTGGCTCCAGGCGATCGAATCGGTCATCCGTGAAGAAGGTGTTGAGCGTGCTCAGTATCTGATCGACCAACTGCTTGCTGAAGCCCGCAAAGGCGGTGTAAACGTAGCCGCAGGCACAGGT  >  NC_000913/122910‑123184                                                                                                                                             |                                                                                                                                       aaGTGCGGCAACTAATCGTAGAACCTGTCTTGTTGCGCTTTCCGGCAAGAGTTCAATGGGACAGGTTCCAGAAAACTCAGCGTTATTAGATAGTGATGGAATCACCCATGTCAGAACGTTTCCCAAATGACGTGGATCCGTTc                                                                                                                                      <  2:491746/143‑1 (MQ=255)     gcgGCAACTAAACGTAGAAGCTGGGTTACTGGGCTTTCGGGCGAGAGTTGAATGGGACGGGTTCCAGAAAAGGCAACGTTATTAGATAGATAAGGAATAACCCATGTCAGAACGTTTCCCAAATGACGTGGATCCGTTCGaaa                                                                                                                                  <  2:575198/143‑1 (MQ=255)     gcgGCAACTAAACGTAGAACCGCTCGTATTGAGCTTTCCGGCGAGAGTTCAATGGGACAGGTTCCAGAAAACTCAACGTTATTAGATAGATAAGGAATAACCCATGTCAGAACGTTTCCCAAATGACGTGGATCCGTTCGaaa                                                                                                                                  <  2:924401/143‑1 (MQ=255)        gCAACTAAACGTAGAACCTGTCTTATTGAGCTTTCCGGCGAGAGTTCAATGGGACAGGTTCCAGAAAACTCAACGTTATTAGGTGGTGGACGAACAACCCATGTCAGAACGTTTCCCAAATGACGTGGATCCGTTCGAAACta                                                                                                                               >  1:903671/1‑142 (MQ=255)                aCGTAGACCCTGTCTTATGGAGCTTGCCGGCGAGAGTTCAATGGGACAGGTTCCAGAAAACTCAACGTTATTAGATTGATAAGGAATAACCCATGTCAGAACGTTTCCCAAATGACGTGGATCCGTTCGAAACTCGCGACTgg                                                                                                                       <  1:256592/143‑1 (MQ=255)                    aGAACCTGTCTTATTGAGCTTTCCGGCGAGAGTTCAATGGGACAGGTTCCAGAAAACTCAACGTTATTAGATAGATAAGGAATAACCCATGTCAGAACGTTTCCCAAATGACGTGGATCCGTTCGAAACTCGCGACTGGCTcc                                                                                                                   <  2:1023486/143‑1 (MQ=255)                       aCCTGTCTTATTGAGCTTTCCGGCGAGAGTTCAATGGGACAGGTTCCAGAAAACTCAACGTTATTAGATAGATAAGGAATAACCCATGTCAGAACGTTTCCCAAATGACGTGGATCCGTTCGAAACTCGCGACTGGCTCCAgg                                                                                                                >  1:192390/1‑143 (MQ=255)                             cTTATTGAGCTTTCCGGCGAGAGTTCAATGGGACAGGTTCCAGAAAACTCAACGTTATTAGATAGATAAGGAATAACCCATGTCAGAACGTTTCCCAAATGACGTGGATCCGTTCGAAACTCGCGACTGGCTCCAGGCGATCg                                                                                                          <  1:304442/143‑1 (MQ=255)                                 ttGAGCTTTCCGGCGAGAGTTCAATGGGACAGGTTCCAGAAAACTCAACGTTATTAGATCGATAAGGAATAACCCATGTCAGAACGTTTCCCAAATGACGTGGATCCGTTCGAAACTCGCGACTGGCTCCAGGCGATCGCATc                                                                                                      >  1:1142795/1‑143 (MQ=255)                                   gAGCTTTCCGGCGAGAGTTCAATGGGACAGGTTCCAGAAAACTCAACGTTATTAGATAGATAAGGAATAACCCATGTCAGAACGTTTCCCAAATGACGTGGATCCGTTCGAAACTCGCGACCGGCTCCAGGCGCTCGAATCgg                                                                                                    >  2:355217/1‑143 (MQ=255)                                    aGCTTTCCGGCGAGAGTTCAATGGGACAGGTTCCAGAAAACTCAACGTTATTAGATAGATAAGGAATAACCCATGTCAGAACGTTTCCCAAATGACGTGGATCCGTTCGAAACTCGCGACTGGCTCCAGGCGATCGAATCgga                                                                                                   >  1:1093900/1‑142 (MQ=255)                                            ggCGAGAGTTCAATGGGACAGGTTCCAGAAAACTCAACGTTATTAGATAGATAAGGAATAACCCATGTCAGAACGTTTCCCAAATGACGTGGATCCGTTCGAAACTCGCGACTGGCTCCAGGCGCTCGACTCGGTCATCCGTg                                                                                           >  1:605385/1‑143 (MQ=255)                                            ggCGAGAGTTCAATGGGACAGGTTCCAGAAAACTCAACGTTATTAGATAGATAAGGAATAACCCATGTCAGAACGTTTCCCAAATGACGTGGATCCGTTCGAAACTCGCGACTGGCTCCAGGCGATCGAATCGGTCATCCGTg                                                                                           <  1:992390/143‑1 (MQ=255)                                             gCGAGAGTTCAATGGGACAGGTTCCAGAAAACTCAACGTTATTAGATAGATAAGGAATAACCCATGTCAGAACGTTTCCCAAATGACGTGGATCCGTTCGAAACTCGCGACTGGCTCCAGGCGa                                                                                                             >  2:1172811/1‑124 (MQ=255)                                             gCGAGAGTTCAATGGGACAGGTTCCAGAAAACTCAACGTTATTAGATAGATAAGGAATAACCCATGTCAGAACGTTTCCCAAATGACGTGGATCCGTTCGAAACTCGCGACTGGCTCCAGGCGa                                                                                                             <  1:1172811/124‑1 (MQ=255)                                              cGAGAGTTCAATGGGACAGGTTCCAGAAAACTCAACGTTATTAGATAGATAAGGAATAACCCATGTCAGAACGTTTCCCAAATGACGTGGATCCGTTCGAAACTCGCGACTGGCTCCAGGCGATCGAATCGGTCATCCGTgaa                                                                                         <  2:715284/143‑1 (MQ=255)                                                   gTTCAATGGGACAGGTTCCAGAAAACTCAACGTTATTAGATAGATAAGGAATAACCCATGTCAGAACGTTTCCCAAAGGACGTGGATCCGTGCGAAACTCGCGACTGGCTCCAGGCGATCGAATCGGTCATCCGTGAAGAAgg                                                                                    <  2:451933/143‑1 (MQ=255)                                                    ttCAATGGGACAGGTTCCAGAAAACTCAACGTTATTAGATAGATAAGGAATAACCCATGTCAGAACGTTTCCCAAATGACGTGGATCCGTTCGAAACTCGCGACTGGCTCCAGGCGATCGAATCGGTCATCCGTGAAGAAGgt                                                                                   >  2:374649/1‑143 (MQ=255)                                                    ttCAATGGGACAGGTTCCAGAAAACTCAACGTTATTAGATAGATAAGGAATAACCCATGTCAGAACGTTTCCCAAATGACGTGGATCCGTTCGAAACTCGCGACTGGCTCCAGGCGATCGAATCGGTCATCCGTGAAGAAGgt                                                                                   <  2:990128/143‑1 (MQ=255)                                                       caTGGGACAAGTTCCAGAAACCTCAACGTTATTAGATAGATAAGGAATACCCCACGTCAGAACGTTTCCCAAATGACGTGGATCCGTTCGAAACTCGCGACTGGCTCCAGGCGATCGAATCGGTCATCCGTGAAGAAGGTGtt                                                                                <  2:89198/142‑1 (MQ=255)                                                       aaTGGGACAGGTTCCAGAAAACTCAACGTTATTAGATAGATAAGGAATAACCCATGTCAGAACGTTTCCCAAATGACGTGGATCCGTTCGAAACTCGCGACTGGCTCCAGGCGATCGAATCGGTCATCCGTGAAGAAGGTGtt                                                                                <  1:980195/143‑1 (MQ=255)                                                        aTGGGACAGGTTCCAGAAAACTCAACGTTATTAGATAGATAAGGAATAACCCATGTCAGAACGTTTCCCAAATGACGTGGATCCGTTCGAAACTCGCGACAGGCTCCAGGAGAACGAAACGGACATACGTGAAGAAGGAGTTg                                                                               >  1:306153/1‑143 (MQ=255)                                                         tGGGACAGGTTCCAGCAAACTCAACGTTATTCGCTGGTTAAGGAATAACCCATGTCAGAACGTTTCCCAAATGACGTGGATCCGTTCGAAACTCGCGACTGGCTCCAGGCGATCGAATCGGTCATCCGTGAAGAAGGTGTTGa                                                                              <  2:871323/143‑1 (MQ=255)                                                            gACAGGTTCCAGAAAACTCAACGTTATTAGATAGATAAGGAATAACCCATGTCAGAACGTTTCCCAAATGACGTGGATCCGTTCGAAACTCGCGACTGGCTCCAGGCGATCGAATCGGTCAGCCGGGAAGAAGGGGTTGACCg                                                                           >  1:223838/1‑143 (MQ=255)                                                            gACAGGTTCCAGAAAACTCAACGTTATTAGATAGATAAGGAATAACCCATGTCAGAACGGTTACCGAATGACGTGGATCCGTTCGAAACTCGCGACTGGCGCCAGGCGATCGAATGGGTCATCCGTGACGAAAGTGGTGAGCg                                                                           >  1:630966/1‑143 (MQ=255)                                                               aGGTTCCAGAAAACGCAACGTTATTAGATAGATAAGGAATAACCCATGTCAGAACGTTTCCCAAATGACGTGGATCCGTTCGAAACTCGCGACTGGCTCCAGGCGATCGAATCGGTCATCCGTGAAGAAGGTGTTGAGCGTGc                                                                        <  2:324678/143‑1 (MQ=255)                                                                ggTTCCAGAAAACTCAACGTTATTAGATAGATAAGGAATAACCCATGTCAGAACGTTTCCCAAATGACGTGGATCCGTTCGAAACTCGCGACTGGCTCCAGGCGATCGAATCGGTCATCCGTGAAGAAGGTGTTGAGCGTGCt                                                                       <  1:107426/143‑1 (MQ=255)                                                                  ttCCGGAAAACTCAACTTTATTAGATAGAGAAGTATTAAGCGATGTCAGAACGTTTCCCACATGACGTGGAGCCGTTGGAAACTCGCGTCTGGCTCCAGGCGATCGAATCGGTCATCCGTGAAGAAGGTGTTGAGCGTGCTCa                                                                     <  2:1145030/143‑1 (MQ=255)                                                                    ccAGACAACTCAACGTTATTAGATCGATAAGGAATCACCCATGTCAGAACGTTTCCCACATGACGTGGATCTGTTCCAATCTCGCGACTTGCTCCAGGCGATCGAATCGGTCATCCGTGAAGAAGGTGTTGAGCGTGCTCAGt                                                                   <  1:261942/143‑1 (MQ=255)                                                                           aCTCATCGTTATGAGATAGATAAGGAATAACCCCGGTCAGAACGTTTCCCAAATGACGGGGATCCGTTCGAAACTCGCGACTGGCTCCAGGCGATCGAATCGGTCATCCGTGAAGAAGGTGTTGAGCGTGCTCAGTATCTGAt                                                            <  1:323867/143‑1 (MQ=255)                                                                              cAACGTTATTAGATAGATAAGGAATAACCCATGTCAGAACGTTTCCCAAATGACGTGGATCCGTTCGAAACTCGCGACTGGCTCCAGGCGATCGAATCGGTCATCCGTGAAGAAGGTGTTGAGCGTGCTCAGTATCTGATCGa                                                         <  1:423543/143‑1 (MQ=255)                                                                                      ttaGATAGATAAGGAATAACCCATGTCAGAACGTTTCCCAAATGACGTGGATCCGTTCGAAACTCGCGACTGGCTCCAGGCGATCGAATCGGTCATCCGTGAAGAAGGTGTTGAGCGTGCTCAGTATCTGATCGACCAACtgc                                                 <  2:858257/143‑1 (MQ=255)                                                                                       tagggagataAGGAATGACCCAGGTCGGGACGTTTCCCAAATGCCGTGGCTCCGTTCGAAACTCGCGACTGGCTCCAGGCGATGGAATCGGCCATCCGTGAAGAAGGTGTTGAGCGTGCTCAGTATCTGATCGACCAACtgct                                                <  1:88468/138‑1 (MQ=255)                                                                                        agatagatAAGGAATAACCTACGTGAGAACGTGGGTCTATTGACGTGGATCCGTTCGAAACTCGCGACTGGCTCCAGGCGATCGAAGCGGTTATCCGTGAAGAAGGTGTTGAGCGTGCTCAGTATCTGATCGACCAACtgctt                                               >  2:269597/1‑143 (MQ=255)                                                                                        agatagatAAGGAATAACCCATGTCAGAACGTTTCCCAAATGACGTGGATCCGTTGGAAAGTCGCCACTGGCTCCAGGCGATCGAATCGGTACACCATGACGAGGGCGTAGAGCGTGCTCAGTAGCTCCACCACGAACtgctt                                               >  2:1138855/1‑143 (MQ=255)                                                                                        acatggataAGGAATAGCTCATGTCAGAACGTTTCCCAAATGGCGTGTATCCGTTCGAAACTCGCGACTGGGTCCGGGCGATCGAATCGGTCATCCGTGGAGAAGGTGTGGAGCGTGCTCAGTATCTGATCGACCAACtgctt                                               <  2:203625/138‑1 (MQ=255)                                                                                         gatagataAGGAATAACCCATGTCAGAACGTTTCCCAAATGACGTGGATCCGTTCGAAACTCGCGACTGGCTCCAGGCGATCGAATCGGTCATCCGTGAAGAAGGTGGTGAGCGTGCTCAGTATCTGATCGACCAACtgattg                                              >  2:1000280/1‑143 (MQ=255)                                                                                          atagataAGGAATAACCCATGTCAGAACGTTTCCCAAATGACGTGGATCCGTTCGAAACTCGCGACTGGCTCCAGGCGATCGAATCGGTCATCCGTGAAGAAGGTGTTGAGCGTGCTCAGTATCTGGTCGAGCAGCtgcttgc                                             >  1:167054/1‑143 (MQ=255)                                                                                            agataAGGAATAACCCATGTCAGAACGTTTCCCAAATGACGTGGATCCGTTCGAAACTCGCGACTGGCTCCAGGCGATCGAATCGGTCATCCGTGAAGAAGGTGTTGAGCGTGCTCAGTATCTGATCGACCAACTGCTTGCTg                                           <  1:559680/143‑1 (MQ=255)                                                                                            agataAGGAACAACCCATGTCAGAACGTTTCCCAAATGACGTGGATCCGTTCGAAACTCGCGACTGGCTCCAGGCGATCGAATCGGTCATCCGTGAAGAAGGTGTTGAGCGTGCTCAGTATCTGATCGACCAACTGCTTGCTg                                           <  1:765509/143‑1 (MQ=255)                                                                                                aaGGAATAACCCATGTCAGAACGTTTCCCAAATGACGTGGATCCGTTCGAAACTCGCGACTGGCTCCAGGCGATCGAATCGGTCATCCGTGAAGAAGGTGTTGAGCGTGCTCAGTATCTGATCGACCAACTGCTTGCTGAAGc                                       <  1:848469/143‑1 (MQ=255)                                                                                                aaGGAATAACCCAGGGCAGAACGTTGCCCAAATGACGTGGATCCGTTCGAAACTCGCGACTGGCTCCAGGCGATCGAATCGGTCATCCGTGAAGAAGGTGTTGAGCGTGCTCAGTATCTGATCGACCAACTGCTTGCTGAAGc                                       <  1:144297/143‑1 (MQ=255)                                                                                                  ggAATAACCCATGTCAGAACGTTTCCCAAATGACGTGGATCCGTTCGAAACTCGCGACTGGCTCCAGGCGATCGACACGGTCATCCGTCAAGAAGGTGTTGAGCGTGCTCAGCATCTCACCGACCAACTGCTTGCTGAAGccc                                     >  2:54125/1‑143 (MQ=255)                                                                                                  ggAATAACCCATGTCAGAACGTTTCCCAAATGACGTGGATCCGTTCGAAACTCGCGACTGGCTCCAGGCGATCGAATCGGTCATCCGTGAAGAAGGTGTTGAGCGTGCTCAGTATCTGATCGACCAACTGCTTGCTGAAGccc                                     <  1:1139706/143‑1 (MQ=255)                                                                                                   gAATAACCCATGTCAGAACGTTTCCCAAATGACGTGGATCCGTTCGAAACTCGCGACTGGCTCCAGGCGATCGAATCGGTCATCCGTGAAGAAGGTGTTGAGCGTGCTCAGTATCTGATCGACCAACTGCTTGCTGAATCCCg                                    >  2:767258/1‑143 (MQ=255)                                                                                                   gAATAACACATGTCAGAACGTTTCCCAAATGACGTGGATCCGTTCGAAACTCGCGACTGGCTACAGGCGATCGAATCGGTCATCCGTGAAGAAGGTGTTGAGCGTGGTCAGTATCTGGTCGAGAATCTGCTTGATGAAGCTCg                                    >  1:413328/1‑143 (MQ=255)                                                                                                         cccATGTCAGAACGTTTCCCAAATGACGTGGATCCGTTCGAAACTCGCGACTGGCTCCAGGCGATCGAATCGGTCATCGGTGAAGAAGGTGTTGAGCGTGCTCAGTATCTGATCGACCAACTGCTTGCTGAAGCCCGCAAAgg                              >  1:46099/1‑143 (MQ=255)                                                                                                          ccATGTCAGAACGTTTCCCAAATGACGTGGATCCGTTCGAAACTCGCGACTGGCTCCAGGCGATCGAATCGGTCATCCGTGAAGAAGGTGTTGAGCGTGCTCAGTATCTGATCGACCAACTGCTTGCTGAAGCCCGCAAAGGc                             >  2:312090/1‑143 (MQ=255)                                                                                                          ccATGTCAGAACGTTTCCCAAATGACGATTTAACTTGCGAAACTCGTGGATCGAACCAGGCGGTCGAATCGTTCATCCTTGTTGGACGTGTGAATCGTTCTCAGGAGCTGATCGACCAAGTGCTTGCTGAAGGCCGCACAGGc                             >  2:821693/1‑143 (MQ=255)                                                                                                            aTGCCAGAACGTTTCCCAAATGACGTGGATCCGTTCGAACCTCGCGACTGGCTCCAGGCGATCGAATCGGTCATCCGTGAAGAAGGTGTTGAGCGTGCTCAGTATCTGATCGACCAACTGCTTGCTGAAGCCCGCAAAGGCgg                           <  1:1208434/143‑1 (MQ=255)                                                                                                                      gTTTCCCAAATGACGTGGATCCGTTCGAAACTCGCGACTGGCTCCAGGCGATCGAATCGGTCATCCGTGAAGAAGGTGTTGAGCGTGCTCAGTATCTGATCGACCAACTGCTTGCTGAAGCCCGCAAAGGCGGTGTAAACGTa                 >  1:130676/1‑143 (MQ=255)                                                                                                                           ccAAATGACGTGGATCCGTTCGAAACTCGCGACTGGCTCCAGGCGCACGAATCGGGCATCCGTGAAGAAGGTGTTGAGCGTGCGCAGTATCTGATCGACCAACGGCTTGCTGAAGGCCGCAGAGGCGGGGTAAACGTAGCCga            >  1:116313/1‑142 (MQ=255)                                                                                                                           ccAAATGACGTGGATCCGTTCGAAACTCGCGACTGGCTCCAGGCGATCGAATCGGTCATCCGTGAAGAAGGTGTTGAGCGTGCTCAGTATCTGATCGACCAACTGCTTGCTGAAGCCCGCAAAGGCGGTGTACACGTAGCCGc            >  2:701268/1‑143 (MQ=255)                                                                                                                            cAAATGACGTGGATCCGTTCGAAACTCGCGACTGGCTCCAGGCGATCGAATCGGTCATCCGTGAAGAAGGTGTTGAGCGTGCTCAGTATCTGATCGACCAACTGCTTGCTGAAGCCCGCAAAGGCGGTGTAAACGTAGCCGCa           <  1:81340/143‑1 (MQ=255)                                                                                                                             cactGACTTGGAGCCGTTCGAAACTCGCGAGTGGCTCCAGGCGATCGAATCGGTCATCCGTGCAGAAGGTGTTGAGCGTGCTCAGTAGCTGATCGACCAACGGCTTGCTGAAGCCCGCAAAGGCGGTGTAAACGTAGCCGCAg          <  2:155995/140‑1 (MQ=255)                                                                                                                                    gTGGATCCGTTCGAAACTCGCGACTGGCTCCAGGCGATCGAATCGGTCATCCGTGAAGAAGGTGTTGAGCGTGCTCAGTATCTGATCGACCAACTGCTTGCTGAAGCCCGCAAAGGCGGTGTAAACGTAGCCGCAGGCACAgg   <  2:268798/143‑1 (MQ=255)                                                                                                                                     tGGATCCGTTCGAAACTCGCGACTGGCTCCAGGCGATCGAATCGGTCATCCGTGAAGAAGGTGTTGAGCGTGCTCAGTATCTGATCGACCAACTGCTTGCTGAAGCCCGCAAAGGCGGTGTAAACGTAGCCGCAGGCACAGGt  <  1:854604/143‑1 (MQ=255)                                                                                                                                       gATCCGTTCGAAACTCGCGACTGGCTCCAGGCGATCGAATCGGTCATCCGTGAAGAAGGTGTTGAGCGTGCTCAGTATCTGATCGACCAACTGCTTGCTGaa                                         >  2:1042887/1‑102 (MQ=255)                                                                                                                                       gATCCGTTCGAAACTCGCGACTGGCTCCAGGCGATCGAATCGGTCATCCGTGAAGAAGGTGTTGAGCGTGCTCAGTATCTGATCGACCAACTGCTTGCTGaa                                         <  1:1042887/102‑1 (MQ=255)                                                                                                                                             |                                                                                                                                       AAGCGCGGCAACTAAACGTAGAACCTGTCTTATTGAGCTTTCCGGCGAGAGTTCAATGGGACAGGTTCCAGAAAACTCAACGTTATTAGATAGATAAGGAATAACCCATGTCAGAACGTTTCCCAAATGACGTGGATCCGATCGAAACTCGCGACTGGCTCCAGGCGATCGAATCGGTCATCCGTGAAGAAGGTGTTGAGCGTGCTCAGTATCTGATCGACCAACTGCTTGCTGAAGCCCGCAAAGGCGGTGTAAACGTAGCCGCAGGCACAGGT  >  NC_000913/122910‑123184

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑