| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 2,132,787 | A→C | I204S (ATC→AGC) | wcaA ← | putative colanic acid biosynthesis glycosyl transferase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 2,132,787 | 0 | A | C | 97.4% | 112.4 / NA | 38 | I204S (ATC→AGC) | wcaA | putative colanic acid biosynthesis glycosyl transferase |
| Reads supporting (aligned to +/- strand): ref base A (0/0); major base C (21/16); minor base T (0/1); total (21/17) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.47e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.94e-01 | |||||||||||
GATCTGGTAGAGGGTAAACAGCTGATATTTTTTGCTGGCGCGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGAT‑ATGCAGGATCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTATCGAACAAACACTCTTTGAAACGCCATGCCCAGGTAAAGACCTGGTT > NC_000913/2132648‑2132926 | gATCTGGTAGAGCGTAAACAGCTGATATTTTTTGCTGGCGCGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGAT‑ATGCAGGctct < 1:877025/143‑1 (MQ=255) aTCTGGTAGAGGGTAAACAGCTGATATTTTTTGCTGGCGCGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATGTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGAT‑ATGCAGGCTCTg > 1:194548/1‑143 (MQ=255) ggTAGAGGGTAAACAGCTGATATTTTTTGCTGGCGCGGTCGAATTTGTCTTTGTGCTTGCGGGAAAAGTGGAAGTACCGGGAGAATTTTTTCGGTGACGAGGTTATCTGCATTTCACCGTGATTCAT‑ATGCCAGCTCTTCGTc > 2:1124553/1‑143 (MQ=255) aCAGCTGCTAGTTTTTGCGGGCGCGGTCGAGTTTGTCGTTGTGCTGGCGGTAAAAGCGGAAGTACCCGGACAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGAT‑ATGCAGGCTCTGCGTCGCCTCTTCTACt < 1:374975/143‑1 (MQ=255) gATATTTTTTGCGGGCGCGGTCGAATTTGGCTGTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGAT‑ATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCAc < 1:442040/143‑1 (MQ=255) ttttttGCTGGCGCGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGAT‑ATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGtt > 2:904731/1‑143 (MQ=255) gcgagggCGCGGTCGAATGTGCCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGAT‑ATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGcc < 1:137890/138‑1 (MQ=255) gTCGGATGTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGAT‑ATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTccacca < 1:289866/143‑1 (MQ=255) tCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGACTTTTTTCGGTGACGAGGTGATCAGCATTACAACGTGATTGCT‑ATGCAGGCTCTGCCTCGCCTCTTCTACTTCTCACGGCACGCCGTACTccaccac > 2:495900/1‑143 (MQ=255) tCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGAT‑ATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTccaccac > 2:68125/1‑143 (MQ=255) aTTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGAT‑ATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACCCCCCCCCCATc > 2:218951/1‑143 (MQ=255) tGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGAT‑ATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGc < 1:583269/143‑1 (MQ=255) tcggggtgcgtgcGGTAAGAGTGGAAGGACCCGGAGAATTTTTCCGGTGACGAGGTGATCTGCATTTCACCGTGATTGAT‑ATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCAGCACCGTCCGCAg < 1:1080734/138‑1 (MQ=255) gtgggCTGGCGGTGAACGTGGAGGTCGCCGGAGCCTTCTGTCCTTTACGTGGTGATCTGCATTTCACCGTGATTGAT‑ATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGaa < 2:874162/139‑1 (MQ=255) gtgCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGAT‑ATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCGCCATCCGCAGGgaga > 1:1080203/1‑143 (MQ=255) cTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGAT‑ATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGACGt > 2:143536/1‑143 (MQ=255) tGCGGTATAAGTGGCAGTACGCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGAT‑ATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCg < 2:330355/143‑1 (MQ=255) tGCGGTAAAAGTGGAAGTCCCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGAT‑ATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCg < 2:985203/143‑1 (MQ=255) ggTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGAT‑ATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCCTCCGCGGGAAGACGACGtca > 2:1105093/1‑141 (MQ=255) gTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGAT‑ATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAAt > 1:598476/1‑143 (MQ=255) tAAATGTGGAGGTGCCCGGAGATTTTTTTCGGTGCCGAGGTGATCTGCATTGCAGCGTGATTGAT‑ATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATc < 2:694678/143‑1 (MQ=255) aacagctgAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGAT‑ATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATcc < 1:6862/136‑1 (MQ=255) tGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGAT‑ATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCCTCCGCAGGACGATGTCGTAATCCTCTGc > 2:869443/1‑143 (MQ=255) ggAGAATTGTGTCGGTGACGGTGTGATCTGCATGTCACCGTGATTGAT‑ATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGtt < 2:511006/143‑1 (MQ=255) agaATTTTTGCGGTGACGAGGGGATCTGCATTTCACCGTGATTGAT‑ATGCAGGCTCTGCGTCGCCTCTTCTACGTTCCACGGTTCGCCGTACTCCACCACCAGCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCg < 2:850480/143‑1 (MQ=255) agaAGTTTTTGGGTGACGAGGTGATCTGCATTTCATCG‑AATGGTTGATGTTGGCGCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCg < 2:257927/143‑1 (MQ=255) tttttCGGTGACGAGGTGATCTGCATTTCACCGTGATTGAT‑ATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACT‑CACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTATCg > 1:818635/1‑143 (MQ=255) ttCGGTGACGAGGTGATCTGCATTTCACCGTGATTGAT‑ATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCAAGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTATCGaa > 1:492630/1‑143 (MQ=255) tGACGAGGTGATCTGCATTTCACCGTGATTGAT‑ATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTACTCCTGTGCCGCTTTCCGTTCGGTATCGaacaaac > 2:301356/1‑143 (MQ=255) aCGAGGTGATCTGCATTTCACCGTGATTGAT‑ATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTATCGACCAAacac > 1:882984/1‑143 (MQ=255) aCGAGGTGATCTGCATTTCACCGTGATTGAT‑ATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTATCGAACAAacac > 1:1077272/1‑143 (MQ=255) cGAGGTGATCTGCATTTCACCGTGATTGAT‑ATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGCAGCTGTCGTAATCCTGTGCCGCTGTCAGTTCGGTAGCGGCCAAACAct > 2:1055356/1‑143 (MQ=255) aGGTGAGCGGCATTTCACCGGGATTGTT‑AGGCAGGCCCTGCGTCGCCTCTTCTACTGTCCGCGGTTCGCCGTACTCCACCTCCATCCGCAGGAAGATGTCGTAAGCCTGTGCCGCTTTCAGTTCGGTATCGAACAAACActct < 2:762406/143‑1 (MQ=255) aTCTGCATTTCACCGTGATTGAT‑ATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGACGCTCTCACTTCGGTATCGAACAAACAATATTTGaa > 2:929577/1‑143 (MQ=255) aCCGTGATTGAT‑ATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGAATCGAACAAACACTCTTTGAAAGGCCATGcgc > 2:641005/1‑141 (MQ=255) ttgat‑ATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTATCGAACAAACACTCTTTGAAACGCCATGCCCAGGTaaa < 1:78481/143‑1 (MQ=255) gat‑ATGCAGGCTCTGCGTCGCCAATTCTACTTTCCAAGGTTCGCCGTCCTCCAACACCATCCGCAGGACGATGTCGTAATCCTGTGCCGCATTCAGTTAGGTATCGAACAAACGCCGTTTGAAAAGCCCTACCCCGGTaaaca > 2:709021/1‑141 (MQ=255) at‑atGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTATCGAACAAACACTCTTTGAAACGCCATGCCCAGGTAAAgcc > 1:1086167/1‑141 (MQ=255) aGGTTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCTGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGTCGCTTTCAGTTCGGTATCGAACAAACACTCTTTGAAACGCCATGCCCAGGTAAAGACCTGGtt < 2:569821/143‑1 (MQ=255) | GATCTGGTAGAGGGTAAACAGCTGATATTTTTTGCTGGCGCGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGAT‑ATGCAGGATCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTATCGAACAAACACTCTTTGAAACGCCATGCCCAGGTAAAGACCTGGTT > NC_000913/2132648‑2132926 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |