| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 2,447,639 | G→A | H268Y (CAT→TAT) | prmB ← | 50S ribosomal subunit protein L3 N(5)‑glutamine methyltransferase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 2,447,639 | 0 | G | A | 96.4% | 150.3 / NA | 57 | H268Y (CAT→TAT) | prmB | 50S ribosomal subunit protein L3 N(5)‑glutamine methyltransferase |
| Reads supporting (aligned to +/- strand): ref base G (1/1); new base A (31/23); total (32/25) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
TGTTTACTTAATCTTTATAAATCGCGAAATGTTCTCGTGCGGCAATAAGCTGCTCTTTGGTGAGCATAAACACACCATCGCCGCCGTTATCAAACTCCAGCCAGGTGAACGGAACATCCGGATATTGTTCCATAAGATGTACCATGCTGTTGCCGACTTCACAAATCA‑‑ACACGCCATCATCAGCAAGGTAATCTGCCGCGTTACCGAGAATGCGACGCGTCAGTTTCAGGCCGTCAGTGCCAGATGCCAGGCCCAGTTCCGGCTCGTGGCGGTATTCGT > NC_000913/2447501‑2447779 | tgtTTACTTAATCTTTATAAATCGCGAAATGTTCTCGTGCGGCAATAAGCTGCTCTTTGGTGAGCATAAACACACCATCGCCGCCGTTATCAAACTCCAGCCAGGTGAACGGAACATCCGGATATTGTTCCATAAGATATAcc > 1:1158927/1‑143 (MQ=255) gtTTACTTAATCTTTATAAATCGCGAAATGTTCTCGTGCGGCAATAAGCTGCTCTTTGGTGAGCATAAACACACCATCGCCGCCGTTATCAAACTCCAGCCAGGTGAACGGAACATCCGGATATTGGTCCATAAGATATACCa > 2:910698/1‑143 (MQ=255) tttACTTAATCTTTATAAATCGCGAAATGTTCTCGTGCGGCAATAAGCTGCTCTTTGGTGAGCATAACCACACCATCGCCGCCGTTATCAAACTCCAGCCAGGTGAACGGAACATCCGGATATTGTTCCATAAGATATACCAt < 1:635829/143‑1 (MQ=255) ttccTTAATCTTTATTAATCGCGAAATGTTCTCGTGCGGCAATAAGCTGCTCTTTGGTGAGCATAAACACACCATCGCCGCCGTTATCAAACTCCAGCCAGGTTAACGGAACATCCGGATATTGTTCCATAAGATATACCATg < 2:784027/140‑1 (MQ=255) ccTTAATCGTTATAAATCGCGAAATGTTCTCGTGCGGCAATAAGCTGCTCTTTGGTGAGCATAAACACACCATCGCCGTCGTTATCAAACTCCAGCCAGGTGAACGGAACATCCGGATATTGTTCCATAAGATATACCATGCt < 1:270124/142‑1 (MQ=255) cTTAATCTTTATAAATCGCGAAATGTTCTCGTGCGGCAATAAGCTGCTCTTTGGTGCTCATAAACACACCATCGCCGCCGTTATCAAACTCCAGCCAGGTGAACGGCACATCCGGATATTGTTCCATAAGATATACCATgatc > 1:964344/1‑140 (MQ=255) ttAATCTTTATAAATCGCGAAATGTTCTCGTGCGGCAATAAGCTGCTCTTTGGTGAGCATAAACACACCATCGCCGCCGTTATCAAACTCCAGCCAGGTGAACGGAACATCCGGATATTGTTCCATAAGATATACCATGCTGt > 1:754510/1‑143 (MQ=255) taaaTCTTTATAAATCGCGAAATGTTCTCGTGCGGCAATAAGCTGCTCTTTGGTGAGCATAAACACACGAGCGACGCCGTTATCAAACTCCAGCCAGGTGAGCGGAACATCCGGATATAATTACATAAGATATACCATGCTGt > 2:1175369/3‑143 (MQ=255) aaTCTTTATAAATCGCGAAATGTTCTCGTGCGGCAATAAGCTGCTCTTTGGTGAGCATAAACACACCATCGCGGCCGTTATCAAACTCCAGCCAGGAGAACGGAACATCCGGATAATGTTCCATAACATATACCATGCAGTTg > 2:586288/1‑143 (MQ=255) aTCCTTATATATCGCGACATGTTCTCTTGCGGCAATAAGCTGCTCTTTGGTGAGCATAAACACACCATCGCCGCCGTTATCAAACTCCAGCCAGGTGAACGGAACATCCGGATATTGTTCCATAAGATATACCATGCTGTTGc < 2:1104462/143‑1 (MQ=255) tttATAAATCGCGAAATGTTCTCGTGCGGCAATAAGCTGCTCTTTGGTGAGCATAAACACACCATCGCCGCCGTTATCAAACTCCAGCCAGGTGAACGGAACATCCGGATATTGTTCCATACGATATACCATGCTGTTGCCGa > 2:453426/1‑143 (MQ=255) agaaaTCGCGGAATGTCGTCGTGCCGCAATATGCTGCTCTTTGGTGAGCATAAACACACCATCGCCGCCGTCAACCGACTACACTCAGGTCAACGGAACATCCGGATATTGTCCCATAAGATATACCATGCTGTTGCCGACtt < 2:551425/141‑1 (MQ=255) aaaTCGCGAAATGTTCTCGTGCGGCAATAAGCTGCTCTTTGGTGAGCATAAACACACCATCGCCGCCGTTATCAAACTCCAGCCAGGTGAACGGCACATCCGGATATTGTTCCATAAGATATACCATGCCGTTGCCGACTTca > 1:293234/1‑143 (MQ=255) tCGCGACATGTTCTCGCGCGGCCAGATGCTGCTCTTTGGTGAGCATAAACACACCATCGCCGCCGTTATCAAACTCCAGCCAGGTGAACGGAACATCCGGATATTGTTCCATAAGATATACCATGCTGTTGCCGACTTCACaa < 2:486872/143‑1 (MQ=255) gAAATGTTCTCGTGCGGCAATAAGCTGCTCTTTGGTGAGCATAAACACACCATCGCCGCCGTTATCAAACTCCAGCCAGGTGAACGGAACATCCGGGTATTGTTCCATAAGCTATACCCTGCTGTTTACGAGTTCAAAAAACa > 1:762239/1‑143 (MQ=255) gAAATGTTCTCGTGCGGCAATAAGCTGCTCTTTGGTGAGCATAAACACACCATCGCCGCCGTTATCAAACTCCAGCCAGGGGAACGGAACATCCGGATATTGTTCCATCAGATGTACCGTGCTGTGGCCGACCCCACAAACCa > 1:460175/1‑143 (MQ=255) aaaTGTTCTCGTGCGGCAATAAGCTGCTCTTTGGTGAGCATAAACACACCATCGCCGCCGTTATCAAACTCCAGCCAGGTGCACGGAACATCCGGATATTGTTCCATAAGATATACCATGCTGTTGCCGACTTCACAAATCa‑‑a < 1:114365/143‑1 (MQ=255) ttCTCGTGCGGCAATAAGCTGCTCTTTGGTGAGCATAAACACACCATCGCCGCCGTTATCAAACTCCAGCCAGGTGAACGGAACATCCGGATATTGTTCCATAAGATATACCATGCTGTTGCCGACTTCACAAATCA‑‑ACACGc > 1:1091492/1‑143 (MQ=255) gtctcGTGCCGCCAGAAGCTGGCCTGTGGTGAGCATAAACACACCGTCGCCGCCGTTATCAAACTCCAGCCAGGTGAACGGAACATCCGGATATTGTTCCATAAGATATACCATGCTGTTGCCGACTTCACAAATCA‑‑ACACGc < 1:481893/142‑1 (MQ=255) cGGCAAGAAGTCGCTCTTCGGTGAGCATAAACACACCCTCGCCGCCGTTATCAAACTCCAGCCAGGTGAACGGATCATCCGGATATTGTTCCATAAGACATACCATGCTGTTGCCGACTTCACAAATCT‑‑ACACGCCAtcatca < 1:1116816/143‑1 (MQ=255) ggCAATAAGCTGCTCTTTGGTGAGCATAAACACACCATCGCCGCCGTTATCAAACTCCAGCCAGGTGAACGGAACATCCGGATATTGTTCCATAAGATATACCATGCTGTTGCCGACTTCACAAATCA‑‑ACACGCCATCATCAg > 2:924722/1‑143 (MQ=255) gCAATAAGCTGCTCTTTGGTGAGCATAAACACACCATCGCCGCCGTTATCAAACTCCAGCCAGGTGAACGGAACATCCGGATATTGTTCCATAAGATATACCATGCTGTTGCGGACTTCCCACATCA‑‑ACACGCCataatccac > 1:1167492/1‑140 (MQ=255) aTAAGCTGCTCTTTGGTGAGCATAAACACACCATCGCCGCCGTTATCAAACTCCAGCCAGGTGAACGGAACATCCGGATATTGTTCCATAAGATATACCATGCTGTTGCCGACTTCACAAATCA‑‑ACACGCCATCATCAGCAAg < 1:128313/143‑1 (MQ=255) tAAGCTGCTCTTTGGTGAGCATAAACACACCATCGCCGCCGTTATCAAACTCCAGCCAGGTGAACGGAACATCCGGATATTGTTCCATAAGATATACCATGCTGTTGCCGACTTCACAAATCA‑‑ACACGCCATCATCAGCAAgg > 2:308831/1‑143 (MQ=255) aGCTGCTCTTTGGTGAGCATAAACACACCATCGCCGCCGTTATCAAACTCCAGCCAGGTGAACGGAACATCCGGATATTGTTCCATAAGATATACCATGCTGTTGCCGACTTCACAAATCA‑‑ACACGCCATCATCAGCAAGGTa < 1:1239545/143‑1 (MQ=255) aGCTGCTCTTTGGTGAGCATAAACACACCATCGCCGCCGTTATCAAACTCCAGCCAGGTGAACGGAACATCCGGATATTGTTCCATAAGATATACCATGCTGTTGCCGACTTCACAAATCA‑‑ACACGCCATCATCAGCAAGGTa < 1:1239644/143‑1 (MQ=255) tctTTGGTGAGCATAAACACACCATCGCCGCCGTTATCAAACTCCAGCCAGGTGAACGGAACATCCGGATATTGTTCCATAAGATATACCATGCTGTTGCCGACTTCACAAATCA‑‑ACACGCCATCATCAGCAAGGTAATCTGc > 1:347163/1‑143 (MQ=255) tcgttGGTTAGCATTAACACACCATCGCCGCCGTTATCAAACTCCAGCCAGGTGAACGGAACATCCGGATATTGTTCCATAGGATCTACCATGCTGTTGCCGACTTCACAAATCA‑‑ACACGCCATCATCAGCAAGGTAATCTGc < 2:536792/140‑1 (MQ=255) ttGGTGAGCATAAACACACCATCGCCGCCGTTATCAAACTCCAGCCAGGTGAACGGAACATCCGGATATTGTTCCATAAGATATACCATGCTGTTGCCGACTTCACATATCA‑‑ACACGCCATCATCAGCAAGGTAATCTGCcgc < 1:353705/143‑1 (MQ=255) tGAGCATAAACACACCATCGCCGCCGTTATCAAACTCCAGCCAGGTGAACGGAACATCCGGATATTGTTCCATAAGATATACCATGCTGTTGCCGCCTTCACAAATCA‑‑ACACGCCATCATCAGCAAGGAAATCTGCCGCGttg > 2:417105/1‑142 (MQ=255) tGAGCATAAACACACCATCGCCGCCGTTATCAAACTCCAGCCAGGTGAACGGAACATCCGGATATTGTTCCATAAGATATACCATGCTGTTGCCGACTTCACAAATCA‑‑ACACGCCATCATCCGCAGCGCAATCCGCCGCGttg > 2:345122/1‑142 (MQ=255) aGCATAAACACACCATCGCCGCCGTTATCAAACTCCAGCCAGGTGAACGGAACAACCGGGTATTGTACCATAAGAGAGACCATGCTGTTGCCGAATTCACAAATCA‑‑ACACGCCCTCATCAGCAAGGTAATATGCCACGTGAcc > 2:748935/1‑143 (MQ=255) tAAACCCACTATCGCCGCCGTTATCAAACTCCAGCCAGGTGAACGGAACATCCGGATATTGTTCCATAAGATATACCATGCTGTTGCCGACTTCACAAATCA‑‑ACACGCCATCATCAGCAAGGTAATCTGCCGCGTTACCgaga < 2:40586/143‑1 (MQ=255) tAAACACACCATCGCCGCCGTTATCAAACTCCAGCCAGGTGAACGGAACATCCGGATATTGTTCCATAAGATATACCATGCTGTTGCCGACTTCACAAATCA‑‑ACACGCCATCATCAGCCAGGTAATCTGCCGCCTTCCCggga > 2:157454/1‑143 (MQ=255) cacacCACCGCGGCGGTTATCAAACACCAGCAAGGTGAACGGAACATCCGGATATTGTTCCATAAGATATACCATGCTGTTGCCGACTTCACAAATCA‑‑ACACGCCATCATCAGCAAGGTAATCTGCCGCGTTACCGAGAATGc < 1:454803/143‑1 (MQ=255) acacCATCGCCGCCGTTATCAAACTCCAGCCAGGTGAACGGAACATCCGGATATTGTTCCATAAGATATACCATGCTGTTGCCGCCTTCACAAATCA‑‑ACACGCCATCATCAGCAAGGTAATCTGCCGCGTTACCGAGAATGCg > 1:1006131/1‑143 (MQ=255) gccgcgGTTATCAAACTCCAGCCAGGTGAACGGAACATCCGGATATTGTTCCATAAGATATACCATGCTGTTGCCGACTTCACAAATCA‑‑ACACGCCAGCATCAGCAAGGTAATCTGCCGCGTTACCGAGAATGCGACGCGTCa < 2:120367/143‑1 (MQ=255) ccgccgTTATCAAACTCCAGCCAGGTGAACGGAACATCCGGATATTGTTCCATAAGATATACCATGCTGTTGCCGACTTCACAAATCA‑‑ACACGCCATCATCAGCAAGGTAATCTGCCGCGTTACCGAGAATGCGACGCGTCAg > 2:1015743/1‑143 (MQ=255) cgTTATCAAACTCCAGCCAGGTGAACGGAACATCCGGATATTGTTCCATAAGATATACCATGCTGTTGCCGACTTCACAAATCA‑‑ACACGCCATCATCAGCAAGGTAATCTGCCGCGTTACCGAGAATGCGACGCGTCCGTTTc > 1:230062/1‑143 (MQ=255) aaaCTCCAGCCAGGTGAACGGAACATCCGGATATTGTTCCATAAGATATACCATGCTGTTGCCGACTTCACAAATCACCACAAGCAATGTT‑‑GCAAGGTAATCTGCCGCGTTACCGAGAATGCGACGCGGCAGTTTCAGGCCGt > 1:905025/1‑143 (MQ=255) aaaCTCCAGCCAGGTGAACGGAACATCCGGATATTGTTCCATAAGATATACCATGCTGTTGCCGACTTCACAAATCA‑‑ACACGCCATCATCAGCAAGGTAATCTCCCGCGTTACCGAGAATGCGACGCGTCAGTCTCAGGCCGt > 1:911370/1‑143 (MQ=255) aaCTCCAGCCAGGTGAACGGAACATCCGGATATTGTTCCATAAGATATACCATGCTGTTGCCGACTTCACAAATCA‑‑ACACGCCATCATCAGCAAGGTAATCTGCCGCGTTACCGAGAATGCGACGCGCCAGTTTCAGGCCGTc > 2:1066369/1‑143 (MQ=255) aaCTCCAGCCAGGTGAACGGAACATCCGGATATTGTTCCATAAGATATACCATGCTGTTGCCGACTTCACAAATCA‑‑ACACGCCATCATCAGCAAGGCAATCTGCCGCGTTACCGAGAATGCGCCGCGTCAGTCTCAGGCCGTc > 2:740898/1‑143 (MQ=255) cTCCAGCCAGGTGAACGGAACATCCGGATATGGTTCCATAAGATATACCATGCTGTTGCCGACTTCACGAATCA‑‑ACACGCCATCATCAGCAAGGTAATCGGCCGCGTTACCAAGCAGGCGGCGCGCCAGTTTAAGGACGTCAg > 2:674092/1‑143 (MQ=255) tCCAGCCAGGTGAACGGAGCATCCGGATATTGTTCCATAAGATATACCATGCTGTTGCCGACTTCACAAATCA‑‑ACACGCCATCATCAGCAAGGTAATCTGCCGCGTTACCGAGAATGCGACGCGTCAGTTTCAGGCCGTCAGt < 2:1169745/143‑1 (MQ=255) tCCAGCCAGGTGAACGGAACATCCGGATATTGTTCCATAAGATATACCATGCTGTTGCCGACTTCACAAATCA‑‑ACACGCCATCATCAGCAAGGTAATCTGCCGCGTTAACGAAAAATCGGGGCGTCAGTTTCAGGCCGTCAGt < 2:754510/143‑1 (MQ=255) ccagccagGTGAACGGAACATCCGGATATTGTTCCATAAGATATACCATGCTGTTGCCGAGTTCTCAAATCA‑‑ACACGCCATCATCAGCAAGGTAATCTGCCGCGTTACCGAGAATGCGACGCGTCAGTTTCAGGCCGTCAGTg < 2:1001576/143‑1 (MQ=255) cagccagGTGAACGGAACATCCGGATATTGCTCCATAAGATATACCATGCTGTTGCCGACTTCACAAATCA‑‑ACACGCCATCATCAGCAAGGTAATCTGCCGCGTTACCGAGAATGCGACGCGTCAGTTTCAGGCCGTCAGTGc < 2:1180868/143‑1 (MQ=255) ggAACATCCGGATATTGTTCCATAAGATATACCATGCTGTTGCCGACTTCACAAATCA‑‑ACACGCCATCATCAGCAAGGTAATCTGCCGCGTTACCGAGAATGCGACGGGTCAGCTTCAGGCCCTCAGGCCCAGATCCCAGGcc > 2:640950/1‑143 (MQ=255) gAACATCCGGATATTGTTCCATAAGATATACCATGCTGTTGCCGACTTCACAAATCA‑‑ACACGCCATCATCAGCAAGGTAATCTGCCGCGTTACCGAGAATGCGACGCGTCAGTTTCAGGCCGTCAGTGCCAGATGCCAGGccc > 1:577706/1‑143 (MQ=255) ccGGATATTGTTCCATAAGATATACCATGCTGTTGCCGACTTCACAAATCA‑‑ACACGCCATCATCAGCAAGGTAATCTGCCGCGTTACCGAGAATGCGACGCGTCCGTTTCAGGCCGTCAGTGCCCGATGCCAGGCCCAGTTcc > 1:545311/1‑143 (MQ=255) ccGGATATTGTTCCATAAGATATACCATGCTGTTGCCGACTTCACAAATCA‑‑ACACGCCATCATCAGCAAGGTAATCTGCCGCGTTACCGAGAATGCGACGCGTCAGTTTCAGGCCGTCAGTGCCAGATGCCAGGCCCAGTTcc > 1:540584/1‑143 (MQ=255) ggATATTGTTCCATAAGATATACCATGCTGTTGCCGACTTCACAAATCA‑‑ACACGCCATCATCAGCAAGGTAATCTGCCGCGTTACCGAGAATGCGACGCGTCAGTTTCAGGCCGTCAGTGCCAGATGCCAGGCCCAGTTCCgg < 1:308831/143‑1 (MQ=255) tGTTCCATAAGATATACCATGCTGTTGCCGACTTCACAAATCA‑‑ACACGCCATCATCAGCAAGGTAATCTGCCGCGTTACCGAGAATGCGACGCGTCAGTTTCAGGCCGTCAGTGCCAGATGCCAGGCCCAGTTCCGGCTCGTg < 1:729355/143‑1 (MQ=255) tGTTCCATAAGATATACCATGCTGTTGCCGACTTCACAAATCA‑‑ACACGCCATCATCAGCAAGGTAATCTGCCGCGTTACCGAGAATGCGACGCGTCAGTTTCAGGCCGTCAGTGCCAGATGCCAGGCCCAGTTCCGGCTCGTg > 1:617970/1‑143 (MQ=255) gTTCCATAAGATATACCATGCTGTTGCCGACTTCACAAATCA‑‑ACACGCCATCATCAGCAAGGTAATCTGCCGCGTTACCGAGAATGCGACGCGTCAGTTTCATGCCGTCAGTGCCAGATGCCAGGCCCAGTTCCGGCTCGTgg < 1:910698/143‑1 (MQ=255) aTGTCCCAGTCTGTTGCCGACTTCACAAGTCC‑‑ACACGCCATCATCAGCAAGGTAAGCTGCCGCGTTCCCGAGAACGCGGCCGGTCAGTTTCAGGCCGTCAGTGCCAGATGCCAGGCCCAGTTCCGGCTCGTGGCGGTATTCGt < 2:815134/143‑1 (MQ=255) | TGTTTACTTAATCTTTATAAATCGCGAAATGTTCTCGTGCGGCAATAAGCTGCTCTTTGGTGAGCATAAACACACCATCGCCGCCGTTATCAAACTCCAGCCAGGTGAACGGAACATCCGGATATTGTTCCATAAGATGTACCATGCTGTTGCCGACTTCACAAATCA‑‑ACACGCCATCATCAGCAAGGTAATCTGCCGCGTTACCGAGAATGCGACGCGTCAGTTTCAGGCCGTCAGTGCCAGATGCCAGGCCCAGTTCCGGCTCGTGGCGGTATTCGT > NC_000913/2447501‑2447779 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |