Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,512,749 G→A intergenic (‑43/‑293) mntH ← / → nupC Mn(2(+))/Fe(2(+)): H(+) symporter MntH/nucleoside:H(+) symporter NupC

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,512,7490GA96.7% 180.5 / NA 60intergenic (‑43/‑293)mntH/nupCMn(2(+))/Fe(2(+)): H(+) symporter MntH/nucleoside:H(+) symporter NupC
Reads supporting (aligned to +/- strand):  ref base G (0/0);  major base A (32/26);  minor base T (1/1);  total (33/27)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TAACCAATCGCCGCAATGAACGCAGGTCCCATTAATGCGAGCCTCATCTTGCGCGCCGCCCGTCCGCTGCTACTCTCAACGCGATAGTTCGTCATCTTGTGCCTCTAAAACATAGCCTTTGCTATGTTTCATGCTATGCCAAACGAGAATGATTATCAAATTCATTTAAATGGATTGTGGTGATTTCTCTGATAGACCAGGATTATGACTACGAAAAGATTGATGACTTGAATGTTATGGTTATGTTTAATGTTAGCACATTTACATAACTTTCA  >  NC_000913/2512612‑2512886
                                                                                                                                         |                                                                                                                                         
tacccAATCGCCGCAATGAACGCAGGTCCCATTAATGCGAGCCTCATCTTGCGCGCCGCCCGTCCGCTGCTACTCTCAACGCGATAGTTCGTCATCTTGTGCCTCTAAAACATAGCCTTTGCTATGTTTCATGCTATACCaaa                                                                                                                                      <  2:35495/140‑1 (MQ=255)
  aCCAATCGCCGCAATGAACGCAGGTCCCATTAATGCGAGCCTCATCTTGCGCGCCGCCCGTCCGCTGCTACTCTCAACGCGATAGTTCGTCATCTTGTGCCTCTGAACGATAGCTTTTGCTATGCTTCATGCTATACCAAACg                                                                                                                                    >  2:871730/1‑143 (MQ=255)
  aCCAATCGCCGCAATGAACGCAGGTCCCATTAATGCGAGACTCAACTTGCGCGCCGCCCGTCCGCTGCTACTCTCAACGCGATAGTTCGTCATCTTGTGCCTCTAAAACATAGCCTTTGCTATGTTTCATGCTATACCAACCg                                                                                                                                    >  1:850274/1‑143 (MQ=255)
         gccgcAATGAACGCAGGTCCCATTAATGCGAGCCTCATCTTGCGCGCCGCCCGTCCGCTGCTACTCTCAACGCGATAGTTCGTCATCTTGTGCCTCTAAAACATAGCCTTTGCTATGTTTCATGCTTTTCCAAACCAGAATGa                                                                                                                             >  1:653003/1‑143 (MQ=255)
          ccgcAATGAACGCAGGTCCCATTAATGCGAGCCTCATCTTGCGCGCCGCCCGTCCGCTGCTACTCTCAACGCGATAGTTCGTCATCTTGTGCCTCTAAAACATAGCCTTTGCTATGTTTCATGCTATACCAAACGAGAATGAt                                                                                                                            >  2:892535/1‑143 (MQ=255)
          ccgcAATGAACGCAGGTCCCATTAATGCGAGCCTCATCTTGCGCGCCGCCCGTCCGCTGCTACTCTCAACGCGATAGTTCGTCATCTTGTGCCTCTAAAACATAGCCTTTGCTATGTTTCATGCTATACCAAACGAGAATGAt                                                                                                                            >  2:956177/1‑143 (MQ=255)
                 gAACGCAGGTCCCATTAATGCGAGCCTCATCTTGCGCGCCGCCCGTCCGCTGCTACTCTCAACGCGATAGTTCGTCATCTTGTGCCTCTAAAACATAGCCTTTGCTATGTTTCATGCTATACCAAACGAGAATGATTATCcaa                                                                                                                     >  2:1127664/1‑143 (MQ=255)
                  aaCGCAGGTCCCATTAATGCGAGCCTCATCTTGCGCGCCGCCCGTCCGCTGCTACTCTCAACGCGATAGTTCGTCATCTTGTGCCTCTAAAACATATCCTTTGCTATGTTTCATGCTATACCAAACGAGAATGATTATCAAAt                                                                                                                    <  1:1142704/143‑1 (MQ=255)
                  aaCGCAGGTCCCATTAATGCGAGCCTCATCTTGCGCGCCGCCCGTCCGCTGCTACTCTCAACGCGATAGTTCGTCATCTTGTGCCTCTAAAACATAGCCTTTGCTATGTTTCATGCTATACCAAACGAGAATGATTATCAAAt                                                                                                                    >  1:644106/1‑143 (MQ=255)
                           cccATTAATGCGAGCCTCATCTTGCGCGCCGCCCGTCCGCTGCTACTCTCAACGCGATAGTTCGGCATCTTGTGCCTCTAAAACATAGCCTTTGCTATGTTTCATGCTATACCAAACGAGAATGATTATCAAATTCATTTaaa                                                                                                           <  1:1107308/143‑1 (MQ=255)
                            ccATTAATGCGAGCCTCATCTTGCGCGCCGCCCGTCCGCTGCTACTCTCAACGCGATAGTTCGTCATCTTGTGCCTCTAAAACATAGCCTTTGCTATGTTTCATGCTATACCAAACGAGAATGATTATCAAATTCATTTAAAt                                                                                                          >  1:888659/1‑143 (MQ=255)
                              aTTAATGCGAGCCTCATCTTGCGCGCCGCCCGTCCGCTGCTTCTCTCAACGCGATAGTTCGTCATCTTGTGCCTCTAAAACATAGCCTTTGCTATGTTTCATGCTATACCAAACGAGAATGATTATCAAATTCATTTAAATgg                                                                                                        >  1:808164/1‑143 (MQ=255)
                                     cGAGCCTCATCTTGCGCGCCGCCCGTCCGCTGCTACTCTCAACGCGATAGTTCGTCATCTTGTGCCTCTAAAACATAGCCTTTGCTATGTTTCATGCTATACCAAACGAGAATGATTATCAAATTCATTTAAATGGATTgtgg                                                                                                 >  1:496979/1‑143 (MQ=255)
                                        gCCTCATCTTGCGCGCCGCCCGTCCGCTGCTACTCTCAACGCGATAGTTCGTCATCTTGTGCCTCTAAAACATAGCCTTTGCTATGTTTCATGCTATATCAAACGAGAATGATTATCAAATTCATTTAAATGGATTGTGGTGa                                                                                              <  1:576534/143‑1 (MQ=255)
                                             aTCTTGCGCGCCGCCCGTCCGCTGCTACTCTCAACGCGATAGTTCGTCATCTTGTGCCTCTAAAACATAGCCTTTGCTATGTTTCATGCTATACCAAACGAGAATGGTTATCAAATTCATTTAAATGGATTGTGGTGATTtct                                                                                         <  1:800577/143‑1 (MQ=255)
                                             aTCTTGCGCGCCGCCCGTCCGCTGCTACTCTCAACGCGATAGTTCGTCATCTTGTGCCTCTAAAACATAGCCTTTGCTATGTTTCATGCTATACCAAACGAGAATGATTATCAAATTCATTTAAATGGATTGTGGTGATTtct                                                                                         <  1:555364/143‑1 (MQ=255)
                                              tCTTGCGCGCCGCCCGTCCGCTGCTACTCTCAACGCGATAGTTCGTCATCTTGTGCCTCTAAAACATAGCCTTTGCTATGTTTCATGCTATACCAAACGAGAATGATTATCAAATTCATTTAAATGGATTGTGGTGATTtctc                                                                                        <  1:1078244/143‑1 (MQ=255)
                                              gatgccgcgcCGCCGGTCCGCTGCAACTCTCGACGCGATAGTTCGTCATCTAGTGCCTATAAAACATAGCCGCTGCTAGGTTGCATGCTATACCAAAAGAGAATGATTATCAAACTCATTTAAATGGATTGTGGTGATTtctc                                                                                        <  2:146100/138‑1 (MQ=255)
                                               ctttggcgcCGACCGTTCGCTGCTTCTCTCGACGGGATAGTTCGTCATCTTGTGCCTCTAAAACATAGCCTTTGCTATGTTTCATGCTATACCAGACGAGAATGATTATCAAATTCATTTAAATGGATTGTGGTGATTTctct                                                                                       <  2:726122/138‑1 (MQ=255)
                                               ctggcgcgcCGCCCGTCCGCTGCTACTCTCAACGCGATAGTTCGTCATCTTGTGCCTCTAAAACATAGCCTTTGCTATGTTTCATGCTATACCAAACGAGAATGATTATCAAATTCATTTAAATGGATTGTGGTGATTTctct                                                                                       <  1:1214867/140‑1 (MQ=255)
                                                 tGCGCGCCGCCCGTCCGCTGCTACTCTCAACGCGATAGTTCGTCATCTTGTGCCTCTACAACATAGCCTTTGCTATGTTTCATGCTATACCAAACGAGAATGATTATCAAATTCATTTAAATGGATTGTGGTGATTTctctaa                                                                                     >  2:241974/1‑141 (MQ=255)
                                                 tGCGCGCCGCCCGTCCGCTGCTACTCTCAACGCGATAGTTCGTCATCTTGTGCCTCTAAAACATAGCCTTTGCTATGTTTCATGCTATACCAAACGAGAATGATTATCAAATTCATTTAAATGGATTGTGGTGATTTCTCTGa                                                                                     <  2:230836/143‑1 (MQ=255)
                                                 tGCGCGCCGCCCGTCCGCTGCTACTCTCAACGCGATAGTTAGGCATCTTGTGCCTCTAAAACATAGCCTTTGCTATGTTTGATGCTATACCACACGAGAATGCTTATCAAATTCATTTAAATGGAATGTGGTGATTTCTCTGa                                                                                     >  2:606623/1‑143 (MQ=255)
                                                        cgcccgccCGCTGCTACCCTCAACGCGCTAGTTCGTCATCTTGTGCCTCTAAAACATAGCCTTTGCTATGTTTCATGCTATACCAAACGAGAATGATTATCAAATTCATTTAAATGGATTGTGGTGATTTCTCTGATAGACCa                                                                              <  1:100378/143‑1 (MQ=255)
                                                        cgccCGTCCGCTGCTACTCTCAACGCGATAGTTCGTCATCTTGTGCCTCTAAAACATAGCCTTTGCTATGTTTCATGCTATACCAAACGAGAATGATTATCAAATTCATTTAAATGGATTGTGGTGATTTCTCTGATAGACCa                                                                              <  2:863991/143‑1 (MQ=255)
                                                         gccCGTCCGCTGCTACTCTCAACGCGATAGTTCGTCATCTTGTGCCTCTAAAACATAGCCTTTGCTATGTTTCATGCTATACCAAACGAGAATGATTATCAAATTCATTTAAATGGATTGTGGTGATTTCTCTGATAGACCAg                                                                             <  2:1226386/143‑1 (MQ=255)
                                                                   tgctACTCTCAACGCGATAGTTCGTCATCTTGTGCCTCTAAAACATAGCCTTTGCTATGTTTCATGCTATACCAAACGAGAATGATTATCAAATTCATTTAAATGGATTGTGGTGATTTCTCTGATAGACCAGGATTATGACt                                                                   >  2:724524/1‑143 (MQ=255)
                                                                   tgctACTCTCAACGCGATAGTTCGTCATCTTGTGCCTCTAAAACATAGCCTTTGCTATGTTTCATGCTATACCAAACGAGAATGATTATCAAATTCATTTAAATGGATTGTGGTGATTTCTCTGATAGACCAGGATTATGACt                                                                   >  2:682647/1‑143 (MQ=255)
                                                                       aCTCTCAACGCGATAGTTCGTCATCTTGTGCCTCTAAAACATAGCCTTTGCTATGTTTCATGCTATACCAAACGAGAATGATTATCAAATTAATTTAGATGGATTGTGGTGATTTCTCTGATAGACCAGGATTATGACTaagc                                                               >  2:37174/1‑140 (MQ=255)
                                                                               cgcgATAGTTCGTCATCTTGTGCCTCTAAAACATAGCCTTTGCTATGTTTCATGCTATACCAAACGAGAATGATTATCAAATTCATTTAAATGGATTGTGGTGATTTCTCTGATAGACCAGGATTATGACTACGAAAAGATtg                                                       >  1:85674/1‑143 (MQ=255)
                                                                                gcgATAGTTCGTCATCTTGTGCCTCTAAAACATAGCCTTTGCTATGTTTCATGCTATACCAAACGAGAATGTTTATCAAATTCATTTAAATGGATTGTGGTGATTTCTCTGATAGACCAGGATTATGACTACGAAAAGATtga                                                      >  2:445687/1‑143 (MQ=255)
                                                                                gcgATAGTTCGTCATCTTGTGCCTCTAAAACATAGCCTTTGCTATGTTTCATGCTATACCAAACGAGAATGATTATCAAATTCATTTAAATGGATTGTGGTGATTTCTCTGATAGACCAGGATTATGACTACGAAAAGATtga                                                      >  2:722466/1‑143 (MQ=255)
                                                                                 cgATAGTTCGTCATCTTGTGCCTCTAAAACATAGCCTTTGCTATGTTTCATGCTATACCAAACGAGAATAATTATCAAATTCATTTAAATCGCTTGTGGTGATTTCTCTGATAGACCAGGATTATGACTACGAAAACATggat                                                     >  2:1125411/1‑143 (MQ=255)
                                                                                 cgATAGTTCGTCATCTTGTGCCGCTAAAACAGAGCCTTTGCTATGTTTCATGCTATACCAAACGAGAATGATTATCAAATTCATTTAAATGGATTGTGGTGATTTCTCTGATAGACCAGGATTATGACTACGAAAAGATtgat                                                     <  1:687584/143‑1 (MQ=255)
                                                                                     aGTTTGTCATTTTGGGCTTATGAAACGTAGACTTTGCTATGTTTCATGCTATACCGAACGAGAATGATTATCAAATTCATTTAAATGGATTGTGGTGATTTCTCTGATAGACCAGGATTATGACTACGAAGATATTGATGACt                                                 <  2:923671/143‑1 (MQ=255)
                                                                                         cGTCATCTTGTGCCTCTAAAACATAGCCTTTGCTATGTTTCATGCTATACCAAACGCGAATGATTATGAAATCCATTTAGATGCATAGTGGTGATTTCTCTGATAGACCAGGATTATGACTGCGAAAAGATTGACGACGTgga                                             >  2:717397/1‑141 (MQ=255)
                                                                                           tCATCTTGTGCCTCTAAAACATAGCCTTTGCTATGTTTCATGCTATACCAAACGAGAATGATTATCAAATTCATTTAAATGGATTGTGGTGATTTCTCTGATAGACCAGGATTATGACTACGAAAAGATTGATGACTTGAATg                                           <  1:887816/143‑1 (MQ=255)
                                                                                           tCATCTTGTGCCTCTAAAACATAGCCTTTGCTATGTTTCATGCTATACCAAACGAGAATGATTATCAAATTCATTTAAATGGATTGTGGTGATTTCTCTGATAGACCAGGATTATGACTACGAAAAGATTGATGACTTGAATg                                           >  1:868096/1‑143 (MQ=255)
                                                                                                ttGTTCCTCTAAAACATAGCCTTTGCTATATTTCATGCTATACCAAACTAGAATTATTATCAAATTCATTTAAATGGATTATGGTTATTTCTCTGATAGACCAGGATTATGACTACGAAAAGATTGATGACTTGAATgttatg                                      <  2:145595/143‑1 (MQ=255)
                                                                                                ttGTGCTTCTAAAACATAGCCTTTACTATGTTTTATGATATACCCAACTAGAATGATTATCAAATTCATTTAAATGGATTGTGGTGATTTCTCTGATAGACCAGGATTATGACTACGAAAAGATTGATGACTTGAATgttatg                                      <  2:746385/143‑1 (MQ=255)
                                                                                                ttGTGCCTCTAAAACATAGCCTTTGCTATGTTTCATGCTATACCAAACGAGAATGATTATCAAATTCATTTAAATGGATTGTGGTGATTTCTCTGATAGACCAGGATTATGACTACGAAAAGATTGATGACTTGAATgttatg                                      >  1:1049597/1‑143 (MQ=255)
                                                                                                ttGTGCCTCTAAAACATAGCCTTTGCTATGTTTCATGCTATACCAAACGAGAATGATTATCAAATTCATTTAAATGGATTGTGGTGATTTCTCTGATAGACCAGGATTATGACTACGAAAAGATTGATGACTTGAATgttatg                                      <  1:361517/143‑1 (MQ=255)
                                                                                                    gCCTCTAAAACATAGCCTTTGCTATATGTCATTTTCAACCGAACGACAATGATTATCAAATTCATTTAAATTGATTCTGTATTGTCCTCTGATGTACCGAGATTATAACTACTAAATGATAGCGGACTTGAATtttctcgtta                                  >  2:1153062/1‑143 (MQ=255)
                                                                                                       tctAAAACATAGCCTTTGCTATGTTTCATGCTATACCAAACGAGAATGATTATCAAATTCATTTAAATGGATTGTGGTGATTTCTCTGATAGACCAGGATTATGACTACGAAAAGATTGATGACTTGAATGTTATGGTTATGt                               >  1:338850/1‑143 (MQ=255)
                                                                                                         tAAAACATAGCCTTTGCTATGTTTCATGCTATACCAAACGAGAATGATTATCAAATTCATTTAAATGGATTGTGGTGATTTCTCTGATAGACCAGGATTATGACTACGAAAAGATTGATGACTTGAATGTTATGGTTCTGttt                             >  2:857771/1‑143 (MQ=255)
                                                                                                         tAAAACATAGCCTTTGCTATGTTTCATGCTATACCAAACGAGAATGATTATCAAATTCATTTAAATGGATTGTGGTGATTACTCTGATAGACCAGGATTATGACTACGAAAAGTTTGAGGACTTGAATGTTATGGTTATGttt                             >  1:627377/1‑143 (MQ=255)
                                                                                                         tAAAACATAGCCTTTGCTATGTTTCATGCTATACCAAACGAGAATGATTATCAAATTCATTTAAATGGATGGTGGTGATTTCTCTGATAGACCAGGATGATGACTACGAAAAGATTGATGACTTGAATGTTATGGTTATGttt                             >  1:198952/1‑143 (MQ=255)
                                                                                                            aaCATAGCCTTTGCTATGTTTCATGCTATACCAAACGAGAATGATTATCAAATTCATTTAAATGGATTGTGGTGATTTCTCTGATAGACCAGGATTATGACTACGAAAAGATTGATGACTTGAATGTTATGGTTATGTTTAAt                          <  2:1159425/143‑1 (MQ=255)
                                                                                                              cATAGCCTTTGCTATGTTTCATGCTATACCAAACGAGAATGATTATCAAATTCATTTAAATGGATTGTGGTGATTTCTCTGATAGACCAGGATTATGACTACGAAAAGATTGATGACTTTAATGTTATGGTTATGTTTAATGt                        <  2:850274/143‑1 (MQ=255)
                                                                                                                 aGCCTTTGCTATGTTTCATGCTATACCAAACGAGAATGATTATCAAATTCATTTAAATGGATTGTGGTGATTTCTCTGATCGACCAGGATTATGACTACGAAAAGATTGATGACTTGAATGTTATGGTTATGTTTAATGTTAg                     >  2:312852/1‑143 (MQ=255)
                                                                                                                   ccTTTGCTATGTTTCATGCTATACCAAACGAGAATGATTATCAAATTCATTTAAATGGATTGTGGTGATTTCTCTGATAGACCAGGATTATGACTACGAAAAGATTGATGACTTGAATGTTATGGTTATGTTTAATGTTAGca                   >  2:434660/1‑143 (MQ=255)
                                                                                                                         cTATGTTTCATGCTATACCAAACGAGAATGATTATCAAATTCATTTAAATGGATTGTGGTGATTTCTCTGATAGACCAGGATTATGACTACGAAAAGATTGATGACTTGAATGTTATGGTTATGTTTAATGTTAGCACATTTa             >  2:827992/1‑143 (MQ=255)
                                                                                                                         cTATGTTTCATGCTATACCAAACGAGAATGATTATCAAATTCATTTAAATGGATTGTGGTGATTTCTCTGATAGACCAGGATTATGACTACGAAAAGATTGATGACTTGAATGTTATGGTTATGTTTAATGTTAGCACATTTa             >  1:832152/1‑143 (MQ=255)
                                                                                                                          tATGTTTCATGCTATACCAAACGAGAATGATTATCAAATTCATTTAAATGGATTGTGGTGATTTCTCTGATAGACCAGGATTATGACTACGAAAAGATTGATGACTTGAATGTTATGGTTATGTTTAATGTTAGCACATTTAc            >  2:1231701/1‑143 (MQ=255)
                                                                                                                              tttCATGCTATACCAAACGAGAATGATTATCAAATTCATTTAAATGGATTGTGGTGATTTCTCTGATAGACCAGGATTATGACTACGAAAAGATTGATGACTTGAATGTTATGGTTATGTTTAATGTTAGCACATTTACATaa        >  2:783662/1‑143 (MQ=255)
                                                                                                                              tttCATGCTATACCAAACGAGAATGATTATCAAATTCATTTAAATGGATTGTGGTGATTTCTCTGATAGACCAGGATTATGACTACGAAAAGATTGATGACTTGAATGTTATGGTTATGTTTAATGTTAGCACATTTACATaa        <  1:956177/143‑1 (MQ=255)
                                                                                                                              gttCATGCTATACCGAGCGAGAATGATTATCAAATTCATTTAAATGGATTGTGGTGATTTCTCTGATAGACCAGGATTATGACTACGAAAAGATTGATGACTTGAATGTTATGGTTATGTTTAATGTTAGCACATTTACATaa        <  1:892535/142‑1 (MQ=255)
                                                                                                                                  aTGCTATTCCAAACGATAATGATTATCAAATTCATTTAAATGGATTGTGGTGATTTCTCTGATAGACCAGGATTATGACTACGAAAAGATTGATGACTTGTATGTTATGGTTATGTTTAATGTTAGCACATTTACATAACttt    <  2:49802/143‑1 (MQ=255)
                                                                                                                                  aTGCTATACCAAACGAGAATGATTATCAAATTCATTTAAATGGATTGTGGTGATTTCTCTGATAGACCAGGATTATGACTACGAAAAGATTGATGACTTGAATGTTATGGTTATGTTTAATGTTAGCACATTTACATAACttt    <  2:85674/143‑1 (MQ=255)
                                                                                                                                    gCTATACCAAACGAGAATGATTATCAAATTCATTTAAATGGATTGTGGTGATTTCTCTGATAGACCAGGATTATGACTACGAAAAGATTGATGACTTGAATGTTATGGTTATGTTTAATGTTAGCACATTTACATAACTTTCa  <  1:856696/143‑1 (MQ=255)
                                                                                                                                         |                                                                                                                                         
TAACCAATCGCCGCAATGAACGCAGGTCCCATTAATGCGAGCCTCATCTTGCGCGCCGCCCGTCCGCTGCTACTCTCAACGCGATAGTTCGTCATCTTGTGCCTCTAAAACATAGCCTTTGCTATGTTTCATGCTATGCCAAACGAGAATGATTATCAAATTCATTTAAATGGATTGTGGTGATTTCTCTGATAGACCAGGATTATGACTACGAAAAGATTGATGACTTGAATGTTATGGTTATGTTTAATGTTAGCACATTTACATAACTTTCA  >  NC_000913/2512612‑2512886

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑