| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 2,917,603 | T→G | V849V (GTT→GTG) | barA → | sensory histidine kinase BarA |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 2,917,603 | 0 | T | G | 98.3% | 173.4 / ‑5.9 | 59 | V849V (GTT→GTG) | barA | sensory histidine kinase BarA |
| Reads supporting (aligned to +/- strand): ref base T (1/0); new base G (28/30); total (29/30) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.92e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.63e-01 | |||||||||||
TGGTGAACCCGAATGCGACCCTCGACTGGCAACTGGCACTACGCCAGGCAGCAGGAAAAACCGATTTAGCGCGCGATATGCTGCAAATGTTACTCGATTTCCTGCCTGAAGTTCGCAACAAAGTTGAGGAACAGCTGGTTGGAGAAAACCCGGAAGGCCTGGTTGATTTGATTCATAAACTGCATGGCAGTTGCGGCTATAGCGGTGTGCCACGTATGAAGAATCTCTGCCAACTGATCGAACAACAGCTACGTAGCGGTACTAAAGAAGAAGATTT > NC_000913/2917464‑2917740 | tgttaaaCCCGAATGCGACCCTCGACTGGCAACTGGCACTACGCCAGGCAGCAGGAACAACCGATTTAGCGCGCGATATGCTGCAAATGTTACTCGATTTCCTGCCTGAAGTTCGCAACAAAGTTGAGGAACAGCTGGTGGga < 2:100687/138‑1 (MQ=255) gcaCCCGTAGGCGACCCTCGCCTGGTATCTCGCACTACGCCAGGCACCCGGAAAAACCTACTTAGCGCGCGATATGCTGCAAATGTTTCTCGATTTCATGCCTTTAGTTCGCAACAAAGTTGAGGAACAGCTGGTGGGAGaaa < 2:378140/141‑1 (MQ=255) aaCCCAAATGCGACCCTCGACTGGCAACTGGCACTACGCCAGGCAGCAGGAAAAACCGATTTAGCGCGCGATATGCTGCAAATGTTACTCGATTTCCTGCCTGAAGTTCGCAACAAAGTTGAGGAACAGCTGGTGGGAGaaaa > 2:1029761/1‑143 (MQ=255) ccGAATGCGACCCTCGACTGGCAACTGGCACTACGCCAGGCAGCAGGAAAAACCGATTTAGCGCGCGATATGCTGCAAATGTTACTCGATTTCCTGACTGAAGTTCGCAACAAGGTTGAGGAACATCTGGTGGGAGAAAAccc > 2:667729/1‑143 (MQ=255) cGAATGCGACCCTCGACTGGCAACTGGCACTACGCCAGGCAGCAGGAAAAACCGATTTAGCGCGCGATATGCTGCAAATGTTACTCGATTTCCTGCCTGAAGTTCGCAACAAAGTTGAGGAACAGCTGGTGGGAGAAAACCCg > 2:33092/1‑143 (MQ=255) gAATGCGACCCTCGACTGGCAACTGGCACTGCGCCAGGCAGCAGGAAAAACCGATTTAGCGCGCGATATGCTGCAAATGTTACTCGATTTCCTGCCTGAAGTTCGCAACAAAGTTGAGGAACAGCTGGTGGGAGAAAACCCgg < 1:441875/143‑1 (MQ=255) gtGCGACCCTCGGCTGGCAACTGGCACTACGCCAGGCAGCAGGAAAAACCGATTTAGCGCGCGATATGCTGCAAATGTTACTCGATTTCCTGCCTGAAGTTCGCAACAAAGTTGAGGAACAGCTGGTGGGAGAAAACCCGGaa < 2:175302/142‑1 (MQ=255) aTGCGACGCGCGACTGGCAACTGGCACTACGCCAGGCAGCAGGAAAAACCGATTTAGCGCGCGATATGCCGCAAATGTTACTCGATTTCCTGCCTGAAGTTCGCAACAAAGTTGAGGAACAGCTGGTGGGAGAAAACCCGGaa < 1:84993/143‑1 (MQ=255) gACCCTCGACTGGCAACTGGCACTACGCCAGGCAGCAGGAAAAACCGATTTAGCGCGCGATATGCTGCAAATGTTACTCGATTTCCTGCCTGAAGTTCGCAACAAAGTTGAGGAACAGCTGGTGGGAGAAAGCCCGGAAGGcc > 1:678905/1‑143 (MQ=255) ccTCGACTGGCAACTGGCACTACGCCAGGCAGCAGGAAAAACCGATTTAGCGCGCGATATGCTGCAAATGTTACTCGATTTCCTGCCTGAAGTTCGCAACAAAGTTGAGGAACAGCTGGTGGGAGAAAACCCGGAAGGCCTgg > 2:525111/1‑143 (MQ=255) ccTCGACTGGCAACTGGCACTACGCCAGGCAGCAGGAAAAACCGATTTAGCGCGCGAGATGCTGCCCACGTTACGCTAGTTCCTGCCTGAAGTTCGCAACAAGGTTGAGGAACAGCTGGTGGGAGAAAGCACGGCAGGCCTgg > 2:149170/1‑143 (MQ=255) cTCGACTGGCAACTGGCACTACGCCAGGCAGCAGGAAAAACCGATTTAGCGCGCGATATGCTGCAAATGTTACTCGATTTCCTGCCTGAAGTTCGCAACAAAGTTGAGGAACAGCTGGTGGGAGAAAACCCGGAAGGCCTGGt > 2:339328/1‑143 (MQ=255) actggcaactggcaCTACGCCAGGCAGCAGGAAAAACCGATTTAGCGCGCGATATGCTGCAAATGTTACTCGATTTCCTGCCTGAAGTTCGCAACAAAGTTGCGGAACAGGTGGTGGGAGAAAACCAGGAAGGCCTGGttgat > 2:597316/1‑143 (MQ=255) ggcaactggcaCTACGCCAGGCAGCAGGAAAAACCGATTTAGCGCGCGATATGCTGCAAATGTTACTCGATTTCCTGCCTGAAGTTCGCAACAAAGTTGAGGAACAGCTGGTGGGAGAAAACCCGGAAGGGCTGGttgatgtg > 1:183151/1‑143 (MQ=255) gcaactggcaCTACGCCAGGCAGCAGGAAAAACCGATTTAGCGCGCGATATGCTGCAAATGTTACTCGATTTCCTGCCTGAAGTTCGCAACAAAGTTGAGGAACAGCTGGTGGGAGAAAACCCGGAAGGCCTGGgtgatttga > 1:1193569/1‑143 (MQ=255) ggcgCTACGCCAGGCAGCAGGAAAAACCGATTTAGCGCGCGATATGCTGCAAATGTTACTCGATTTCCTGCCTGAAGTTCGCAACAAAGTTGAGGAACAGCTGGTGGGAGAAAACCCGGAAGGCCTGGTTGATTTGATTCATa < 1:107642/143‑1 (MQ=255) aGGCAGCAGGAAAAACCGATTTAGCGCGCGATATGCTGCAAATGTTACTCGATTTCCTGCCTGAAGTTCGCAACAAAAAACCACAGATTATGTTTGGGGAAAACCCGGAAGGCCTGGTTGATTTGATTCATAAACTGCATGGc > 1:283304/1‑143 (MQ=255) gcagcaGGAAAAACCGATTTAGCGCGCGATATGCTGCAAATGTTACTCGATTTCCTGCCTGAAGTTCGCAACAAAGTTGAGGAACAGCTGGTGGGAGAAAACCCGGAAGGCCTGGTTGATTTGATTCATAAACTGCATGGCAg > 2:897389/1‑143 (MQ=255) gcagcaGGAAAAACCGATTTAGCGCGCGATATGCTGCAAATGTTACTCGATTTCCTGCCTGAAGTTCGCAACAAAGTTGAGGAACAGCTGGTGGGAGAAAACCCGGAAGGCCTGGTTGATTTGATTCATAAACTGCATGGCAg < 1:1137920/143‑1 (MQ=255) agcagGAAAAACCGATTTAGCGCGCGATATGCTGCAAATGTTACTCGATTTCCTGCCTGAAGTTCGCAACAAAGTTGAGGAACAGCTGGTGGGAGAAAACCCGGAAGGCCTGGTTGATTTGATTCATAAACTGCATGGCAGtt > 1:620966/1‑143 (MQ=255) ctggAAAATCCTATTTAGCGCGCGATATGCTGCAAATGTGACTCGATTTCCTGCCTGAAGTTCGCAACAAAGTTGAGAACCGGCAGGTGGGAGAAAACCCGGAAGGCCTGGTTGATTTGATTCATAAACTGCATGGCAGTTGc < 1:339441/141‑1 (MQ=255) cagGAAAAACCGATTTAGCGCGCGATATGCTGCAAATGTTACTCGATTTCCTGCCTGAAGTTCGCAACAAAGTTGAGGAACAGCTGGTGGGAGAAAACCCGGAAGGCCTGGTTGATTTGATTCATAAACTGCATGGCAGTTGc > 2:580915/1‑143 (MQ=255) ggAAAAACGCATCTAGCGCGCGGTATGCTGCAAAGGGTACGCGATTTCCTGCCTGAAGTTCGCAACAAAGTTGAGGAACAGCTGGTGGGAGAAAACCCGGAAGGCCTGGTTGATTTGATTCATAAACTGCATGGCAGTTGCgg < 1:1179246/143‑1 (MQ=255) gagagaCCGATTTGGCGCGCGATATGCTGCAAATGTTACTCGATTTCCTGCCTGAAGTTCGCAACAAAGTTGAGGAACAGCTGGTGGGAGAAAACCCGGAAGGCCTGGTTGATTTGATTCATAAACTGCATGGCAGTTGCGGc < 2:636648/138‑1 (MQ=255) aaaaaCCGATTTAGCGCGCGATATGCTGCAAATGTTACTCGATTTCCTGCCTGAAGTTCGCAACAAAGTTGAGGCACAGCTGGTGGGAGAAAACCCGGAAGGCCTGGTTGATTTGATTCATAAACTGCATGGCAGTTGCGGCt > 1:269387/1‑143 (MQ=255) aaaaaCCGATTTAGCGCGCGATATGCTGCAAATGTTACTCGATGTCCTGCCTGAAGTTCGCAACAAAGTTGAGGAACAGCTGTTGGGAGAAAACCCGGAAGGCCTGGTTGATTTGATTCATAAACTGCATGGCAGTTGCGGCt < 1:748902/143‑1 (MQ=255) aaaaCCGATTTAGCGCGCGATATGCTGCAAATGTTACTCGATTTCCTGCCTGAAGTTCGCAACAAGGTTGAGGAACAGCTGGTGGGAGAAAACCCGGAAGGCCTGGTTGATTTGATTCATAAACTGCATGGCAGTTGCGGCta < 2:860583/143‑1 (MQ=255) aaCCGATTTAGCGCGCGATATGCTGCAAATGTTACTCGATTTCCTGCCTGAAGTTCGCAACAAAGTTGAGGAACAGCTGGTGGGAGAAAACCCGGAAGGCCTGGTTGATTTGATTCATACACTGCATGGCAGTTGCGGCtata > 1:246668/1‑143 (MQ=255) aCCGATTTAGCGCGCGATATGCTGCAAATGTTACTCGATTTCCTGCCTGAAGTTCGCAACAAAGTTGAGGAACAGCTGGTGGGAGAAAACCCGGAAGGCCTGGGTGATTTGATTCATAAACTGCATGGCAGTTGCGGCTATAg > 1:1025107/1‑143 (MQ=255) gATTTAGCGCGCGATATGCTGCAACTGTTACTCGATTTCCTGCCTGAAGTTCGCAACAAAGTTGAGGAACAGCTGGTGGGAGAAAACCCGGAAGGCCTGGTTGATTTGATTCATAAACTGCATGGCAGTTGCGGCTATAGCgg > 2:372635/1‑143 (MQ=255) aTTTAGCGCGCGATATGCGGCAAATGTTACTCGATTTCCTGCCTGAAGTTCGCAACAAAGTTGAGGAACAGCTGGTGGGAGAAAACCCGGAAGGCCTGGTTGATTTGATTCATAAACTGCATGGCAGTTGCGGCTATAGCGgt < 1:737155/143‑1 (MQ=255) aTTGAGCGCGCGATATGCTGCAAATGTTACTCGATTTCCTGCCTGAAGTTCGCAACAAAGTTGAGGAACAGCTGGTGGGAGAAAACCCGGAAGGCCTGGTTGATTTGATTCATAAACTGCATGGCAGTTGCGGCTATAGCGgt < 1:745187/143‑1 (MQ=255) ttAGCGTGCGATATGCTGCAAATGTTACTCGATTTCCTGCCTGAAGTTCGCAACAAAGTTGAGGAACAGCTGGTGGGAGAAAACCCGGAAGGCCTGGTTGATTTGATTCATAAACTGCATGGCAGTTGCGGCTATAGCGgtgt < 2:541013/143‑1 (MQ=255) aGCGCGCGATATGCTGCAAATGTTACTCGATTTCCTGCCTGAAGTTCGCAACAAAGTTGAGGAACAGCTGGTGGGAGAAAACCCGGAAGGCCTGGTTGATTTGATTCATAAACTGCGTGGCAGTTGCGGCTAGAGAGGTGTGc > 2:458839/1‑143 (MQ=255) tgcgcgATATGCTGCAAATGTTACTCGATTGCCTGCCTGAAGTTCGCAACAAAGTTGAGGAACAGCTGGTGGGAGAAAACCCGGAAGGCCTGGTTGATTTGATTCATAAACTGCATGGCAGTTGCGGCTATAGCGGTGTGCCa < 2:714182/142‑1 (MQ=255) ctagatGCTGCAAATGTTACGCGATTTCCTGCCTGAAGTTCGCAACAAAGTTGCGGAACAGCTGGTGGGAGAACACCCGGAAGGCCTGGTTGATTTGATTCATAAACTTCATGGCAGTTGCGGCTATAGCGGTGTGCCACGTa < 2:445549/139‑1 (MQ=255) tatGCTGCAAATGTTACTCGATTTCCTGCCTGAAGTTCGCAACAAAGTTGAGGAACAGCTGGTGGGAGAAAACCCGGAAGGCCTGGTTGATTTGATTCATAAACTGCATGGCAGTTGCGGCTATAGCGGTGTGCCACGTATga < 2:290536/143‑1 (MQ=255) tatGCTGCAAATGTTACTCGATTTCCTGCCTGAAAACCTCAACAAAGTTGAGGAACAGCTGGTGGGAGGAAACCCGGAAGGCCTGGGTGATTTGATTCATAAACTGCATGGCAGTTGCGGCTATAGCGGTGTGCCACGTATga > 2:210960/1‑143 (MQ=255) tgcAAATGTTACTCGATTTCCTGCCTGAAGTTCGCAACAAAGTTGAGGAACAGCTGGTGGGAGAAAACCCGGAAGGCCTGGTTGATTTGATTCATAAACTGCATGGCAGTTGCGGCTATAGCGGTGTGCCACGTATGAAGAAt > 1:1080240/1‑143 (MQ=255) gcAAATGTTACTCGATTTCCTGCCTGAAGTTCGCAACAAAGTTGAGGAACAGCTGGTGGGAGAAAACCCGGAAGGCCTGGTTGATTTGATTCATAAACTGCATGGCAGTTGCGGCTATAGCGGTGTGCCACGTACGAAGAAtc > 2:699114/1‑143 (MQ=255) cAAATGTTACTCGATTTCCTGCCTGAAGTTCGCAACAAAGTTGAGGAACAGCTGGTGGGAGAAAACCCGGCAGGCCTGGTTGATTTGATTCATAAACTGCATGGCAGTTGCGGCTATAGCGGTGTGCCACGTATGAAGAAtct > 2:1040336/1‑143 (MQ=255) aaaTGTTACTCGATTTCCTGCCTGAAGTTCGCAACAAAGTTGAGGAACAGCTGGTGGGAGAAAACCCGGAAGGCCTGGTTGATTTGATTCATAAACTGCATGGCAGTTGCGGCTATAGCGGTGTGCCACGTATGAAGAAtctc < 1:1029761/143‑1 (MQ=255) tACTCGATTTCCTGCCTGAAGTTCGCAACAAAGTTGAGGAACAGCTGGTGGGAGAAAACCCGGAAGGCCTGGTTGATTTGATTCATAAACTGCATGGCAGTTGCGGCTATAGCGGTGTGCCACGTATGAAGAATCTCTGCCaa < 1:33092/143‑1 (MQ=255) tACTCGATTTCCTGCCTGAAGTTCGCAACAAAGTTGAGGAACAGCTGGTGGGAGAAAACCCGGAAGGCCTGGTTGATTAGATTCATAAACTGCATGGAAGTTGCGGCTATAGAGGTGTACAACGAATGAAGCACCTCGGCCaa > 2:1001235/1‑143 (MQ=255) cTCGATTTCCTGCCAGAAGTTCGCAACAAAGTTGAGGAACAGCTGGTGGGAGAAAACCCGGAAGGCCTGGTTGATTTGATTCATAAACTGCATGGCAGTTGCGGCTATAGCGGTGTGCCACGTATGAAGAATCTCTGCCAACt < 1:251545/143‑1 (MQ=255) tttCCTGCCTGAAGTTCGCAACAAAGTTGAGGAACAGCTGGTGGGAGAAAACCCGGAAGGCCTGGTTGATTTGATTCATAAACTGCATGGCAGTTGCGGCTATAGCGGTGTGCCACGTATGAAGAATCTCTGCCAACTGATCg < 1:848288/143‑1 (MQ=255) aaGTTCGCAACAAAGTTGAGGAACAGCTGGTGGGAGAAAACCCGGAAGGCCTGGTTGATTTGATTCATAAACTGCATGGCAGTTGCGGCTATAGCGGTGTGCCACGTATGAAGAATCTCTGCCAACTGATCGAACAACAGCTa < 1:922717/143‑1 (MQ=255) cGCAACAAAGTTGAGGAACAGCTGGTGGTAGAAAACCCGGAAGGCCTGGTTGATTTGATTCATAAACTGCATGGCAGTTGCGGCTATAGCGGTGTGCCACGTATGAAGAATCTCTGCCAACTGATCGAACAACAGCTACGTAg < 2:126280/143‑1 (MQ=255) cGCAACAAAGTTGAGGAACAGCTGGTGGGAGAAAACCCGGAAGGCCTGGTTGATTTGATTCATAAACTGCATGGCAGTTGCGGCTATAGCGGTGTGCCACGTATGAAGAATCTCTGCCAACTGATCGAACCACAGCTACGtcg > 1:1196356/1‑141 (MQ=255) gCAACAAAGTTGAGGAACAGCTGGTGGGAGAAAACCCGGAAGGCCTGGTTGATTTGATTCATAAACTGCATGGCAGTTGCGGCTATAGCGGTGTGCCACGTATGAAGAATCTCTGCCAACTGATCGAACAACATCTACGTAGc > 2:151790/1‑143 (MQ=255) aacaaAGTTGAGGAACAGCTGGTGGGAGAAAACCCGGAAGGCCTGGTTGATTTGATTCATAAACTGCATGGCAGTTGCGGCTATAGCGGTGTGCCCCGTATGAAGAATCTCTGCCAACTGATCGAACAACAGCTACGTAGCgg < 1:580915/143‑1 (MQ=255) caaAGTTGAGGAACAGCTGGTGGGAGAAAACCCGGAAGGCCTGGTTGATTTGATTCATAAACTGCATGGCAGTTGCGGCTATAGCGGTGTGCCACGTATGAAGAATCTCTGCCAACTGATCGAACAACAGCTACGTAGCGGTa > 1:665604/1‑143 (MQ=255) aaaGTTGAGGAACAGCTGGTGGGAGAAAACCCGGAAGGCCTGGTTGATTTGATTCATAAACTGCATGGCAGTTGCGGCTATAGCGGTGTGCCACGTATGAAGAATCTCTGCCAACTGATCGAACAACAGCTACGTAGCGGTAc < 1:923937/143‑1 (MQ=255) aaaGGTGAGGAGCAGCGGGGGGGAGAAAGCCCGGAAGGCCTGGTTGATTTGATTCATAAACTGCATGGCAGGTGGGGCTATCGCGGTGTGCCACGTATGAAGAATCTCTGCCAACTGATCGAACAACAGCTACGTAGCGGTAc < 1:424499/143‑1 (MQ=255) tGAGGAATATGTGGTGGCAGAAAACCCGGAAGGCTTTGTTGATGTGATTCATAAACTGCATGGCAGTTGCGGCGATAGCGGTGGGCCACGCATGAAGAATCTCTGCCAACTGATCGAACAACAGCTACGTAGCGGTACTAaag < 2:129011/143‑1 (MQ=255) aGGAACAGCTGGTGGGAGAAAACCCGGAAGGCCTGGTTGATTTGATTCATAAACTGCATGGCAGTTGCGGCTATAGCGGTGTGCCACGTATGAAGAATCTCTGCCGACTGATCGAACAACAGCTGCGTAGCGGAAcagaagga > 1:34341/1‑136 (MQ=255) cAGCTGGTGGGAGAAAACCCGGAAGGCCTGGTTGATTTGATTCATAAACTGCATGGCAGTTGCGGCTATAGCGGTGTGCCACGTATGAAGAATCTCTGCCAACTGATCGAACAACAGCTACGTAGCGGTACTAAagaagaaga > 1:705771/1‑143 (MQ=255) gCTGGTGGGAGAAAGGCGGCGAGGCCTGGTTGATTTGATTCATAAACTGCGTGGCAGTTGCGGCTATAGCGGTGTGCCACGTATGAAGAATCTCTGCCAACTGATCGAACAACAGCTACGTAGCGGTACTAAAGAAGAAGAtt < 2:602085/143‑1 (MQ=255) cTGGTGGGAGAAAACCCGGAAGGCCTGGTTGATTTGATTCATAAACTGCATGGCAGTTGCGGCTATAGCGGTGTGCCACGTATGAAGAATCTCTGCCAACTGATCGAACAACAGCTACGTAGCGGTACTAAAGAAGAAGAttt < 2:620966/143‑1 (MQ=255) | TGGTGAACCCGAATGCGACCCTCGACTGGCAACTGGCACTACGCCAGGCAGCAGGAAAAACCGATTTAGCGCGCGATATGCTGCAAATGTTACTCGATTTCCTGCCTGAAGTTCGCAACAAAGTTGAGGAACAGCTGGTTGGAGAAAACCCGGAAGGCCTGGTTGATTTGATTCATAAACTGCATGGCAGTTGCGGCTATAGCGGTGTGCCACGTATGAAGAATCTCTGCCAACTGATCGAACAACAGCTACGTAGCGGTACTAAAGAAGAAGATTT > NC_000913/2917464‑2917740 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |