Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,041,398	T→G	L29R (CTT→CGT)	ygfZ →	folate‑binding protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,041,398	0	T	G	96.6%	181.4 / NA	60	L29R (CTT→CGT)	ygfZ	folate‑binding protein
Reads supporting (aligned to +/- strand):  ref base T (0/2);  new base G (27/30);  total (28/32)
Fisher's exact test for biased strand distribution p-value = 4.95e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.99e-01

CTCTTTTACCATTAGTCATTAATACGCCGTTAAGCAACTCAGGACTCTATTATGGCTTTTACACCTTTTCCTCCCCGTCAGCCTACGGCTTCTGCCCGTTTACCGCTGACGCTGATGACGCTTGATGACTGGGCGCTTGCCACCATTACTGGCGCGGACAGCGAAAAATATATGCAGGGTCAGGTGACAGCAGATGTCAGCCAGATGGCAGAAGATCAGCACCTGCTCGCCGCCCATTGCGACGCCAAAGGTAAAATGTGGAGCAATTTACGTCTG  >  NC_000913/3041262‑3041537                                                                                                                                         |                                                                                                                                            ctctTTTACCATTAGTCATTAATACGCCGTTAAGCAACTCGGGACTCTATTATGGCATTTACACGTTTTCCTCACCGTCAGCCTGCGGCTTCTGCCCGTGTACGGCGGACGCTGATGACCCTCGATGCCTGGGCGCACGccac                                                                                                                                       >  2:988811/1‑143 (MQ=255) ctctTTTACCATTAGTCATTAATACGCCGTTAAGCAACTCAGGACTCTATTATGGCTTTTACACCTTTTCCTCCCCGTCAGCCTACGGCTTCTGCCCGTTTACCGCTGGCGCTGATGACGCTTGATGACTGGGCGCGTGccac                                                                                                                                       >  2:503634/1‑143 (MQ=255)        cccATTAGTCATTAATACGCCGTTAAGCAACCCAGGACTCTATTATGGCTTTTACACCTTTTCCTCCCCGTCAGCCTACGGCTTCTGCCCGTTTACCGCTGACGCTGATGACGCTTGATGACTGGGCGCGTGCCACCATTACt                                                                                                                                <  1:43348/142‑1 (MQ=255)           actAGTCAACAATACGCCGCAAAGCAACTCAGGACTCTATTATGGCTTTTACACCTTTTCCTCCCCGTCAGCCTACGGCTTCTGCCCGTTTACCGCTGACGCTGATGACGCTTGATGACTGGGCGCGTGCCACCATTACTGgc                                                                                                                             <  1:75402/141‑1 (MQ=255)              agccATTAATACGCCGTTAAGCAACTCAGGACTCTATTATGGCTTTTACACCTTTTCCTCCCCGTCAGCCTACGGCTTCTGCCCGTTTACCGCTGACGCTGATGACGCTTGATGACTGGGCGCGTGCCACCATTACTGGcgcg                                                                                                                          <  1:686920/140‑1 (MQ=255)                tCATTAATACGCCGTTAAGCAACTCAGGACTCTATTATGGCTTTTACACCTTTTCCTCCCCGTCAGCCTACGGCTTCTGCCCGTTTACCGCTGACGCTGATGACGCTTGATGACTGGGCGCGTGCCACCATTACTGGCGCGGa                                                                                                                        >  2:228369/1‑143 (MQ=255)                  aTTAATATGCCGTTAAGCAACTCAGGACTCTATTATGGCTTTTACACCTTTTCCTCCCCGTCAGCCTACGGCTTCTGCCCGTTTACCGCTGACGCTGATGACGCTTGATGACTGGGCGCGTGCCCCCGTTACTGGCGCGGACa                                                                                                                      >  1:11001/1‑143 (MQ=255)                  aTTAATACGCCGTTAAGCAACTCAGGACTCTATTATGGCTTTTACACCTTTTCCTCCCCGTCAGCCTACGGCTTCTGCCCGTTTACCGCTGACGCTGATGACGCTTGATGACTGGGCGCGTGCCACCATTACTGGCGCGGACa                                                                                                                      >  2:690270/1‑143 (MQ=255)                       tACGCCGTTAAGCACCTCCGGACTCTATTATGGCTTTTACACCCTTTCCTCCCCGTCAGCCTACGGCTTCCGCCCTTTTCCCCCTGACGCTGATGACGCTTGATGACTGGGCGCGTGCCACCATTACTTGCGCGGACAGCGaa                                                                                                                 <  2:24977/143‑1 (MQ=255)                         cGCCGTTAAGCAACTCAGGACTCTATTATGGCTTTTACACCTTTTCCTCCCCGTCAGCCTACGGCTTCTGCCCGTTTACCGCTGACGCTGATGACGCTTGATGACTGGGCGCGTGCCACCATTGCTGGCGCGGCGAGCGaaag                                                                                                               >  1:29714/1‑142 (MQ=255)                         cGCCGTTAAGCAACTCAGGACTCTATTATGGCTTTTACACCTTTTCCTCCCCGTCAGCCTACGGCTTCTGCCCGTTTACCGCTGACGCTGATGACGCTTGATGACTGGGCGCGTGCCACCATTACTGGCGCGGACAGCGaaaa                                                                                                               <  1:642277/143‑1 (MQ=255)                                 aGCAACGCAGGACTCTATTATGGCTTTTACACCTTTTCCTCCCCGTCAGCCTACGGCTTCTGCCCGTTTACCGCTGACGCTGATGACGCTTGATGACTGGGCGCGTGCCACCATTACTGGCGCGGACAGCGAAAAATATATGc                                                                                                       <  1:876087/143‑1 (MQ=255)                                   cAACTCAGGACTCTATTATGGCTTTTACACCTTTTCCTCCCCGTCAGCCTACGGCTTCTGCCCGTTTACCGCTGACGCTGATGACGCTTGATGACTGGGCGCGTGCCACCATTACTGGCGCGGACAGCGACAAATATATGccg                                                                                                     >  2:770223/1‑141 (MQ=255)                                   cAACTCAGGACTCTATTATGGCTTTACCTATCATTCCTCCCCGTCAGCCTACGGCTTCTGCCCGTTTACCGCTGACGCTGATGACGCTTGATGACTGGGCGCGTGCCACCATTACTGGCGCGGACAGCGAAAAATATATGCAg                                                                                                     <  1:226771/143‑1 (MQ=255)                                       tCAGGACTCTATTATGGCTTTTACACCTTTTCCTCCCCGTCAGCCTACGGCTTCTGCCCGTTTACCGCTGACGCTGATGACGCTTGATGACTGGGCGCGTGCCACCATTACTGGCGCGGACAGCGAAAAGTATATGCAGGGtg                                                                                                 >  1:379186/1‑142 (MQ=255)                                                cagtatGGCTTTCACACCTTCTCCTCCCCGTCAGCCTACGGCTTCTGCCCGTTTACCGCTGACGCTGATGACGCTTGATGACTGGGCGCGTGCCACCATTACTGGCGCGGACAGCGAAAAATATATGCAGGGTCAGGTGAcag                                                                                        <  2:940864/140‑1 (MQ=255)                                                 attatGGCTTTTACACCTTTTCCTCCCCGTCAGCCTACGGCGTCTGCCCGTTTACCGCTGACGCTGATGACGCTTGATGACTGGGCGCGTGCCACCATTACTGGCGCGGACAGCGAAAAATATATGCAGGGTCAGGTGAcagc                                                                                       >  2:1214662/1‑143 (MQ=255)                                                 attatGGCTGTTGCACCTTTTCCCCCGCGTCAGCCTACGGCTGCTGCCCGTTTACCGCTGACGCTGATGACGCTTGATGACTGGGCGCGTGCCACCATTACTGGCGCGGACAGCGAAAAATATATGCAGGGTCAGGTGAcagc                                                                                       <  2:1136248/143‑1 (MQ=255)                                                 attatGGCGTTTACACCTTTTCCTCCCCGTCAGCCTACGGCTTCTGCCCGTTTACCGCTGACGCTGATGACGCTTGATGACTGGGCGCGTGCCACCATTACTGGCGCGGACAGCGAAAAATATATGCAGGGTCAGGTGAcagc                                                                                       <  1:174355/143‑1 (MQ=255)                                                   tatGGCTTTTACACCTTTACCTCCCCGTCAGCCTACGTCTTCTGCCTGTTTTTTGTTTACACCAAAAACGCTTTTTGACTGGGCGCGTGCCACCATGAAAGGCGCGGACAGCGAAAAATATATGCAGGGTCAGGTGAcagcag                                                                                     <  1:283958/143‑1 (MQ=255)                                                     tGGCTTTTACACCTTTTCCTCCCCGTCAGCCTACGGCTTCTGCCCGTTTACCGCTGACGCTGATGACGCTTGATGACTGGGCGCGTGCCACCATTACTGGCGCGGACAGCGAAAAATATATGCAGGGTCAGGGGACAGCAGag                                                                                   >  1:199863/1‑142 (MQ=255)                                                      ggCTTTTACACCTTTTGCTCCCCGTCAGCCTACGGCTGCTGCCCGTTTACCGCTGACGCTGATGACGGTTGATGACTGGGCGCGTGCCACCATTGCTGGCGCGGACAGCGAAAAATATATGCAGGGTCAGGTGACAGCAGATg                                                                                  <  1:550527/143‑1 (MQ=255)                                                        cTTTTACACCTTTTCCTCCCCGTCAGCCTACGGCTTCTGCCCGTTTACCGCTGACGCTGATGACGCTTGATGACTGGGCGCGTGCCACCATTACTGGCGCGGACAGCGAAAAATATATGCAGGGTCAGGTGACAGCAGATGTc                                                                                <  1:1152194/143‑1 (MQ=255)                                                         ttttACCTCTTTTCCTCCCCGTCCGCCTACGGCTTCTGCCCGTTTACCGCTGACGCTGATGACGCTTGATGACTGGGCGCGTGCCACCATTACTGGCGCGGACAGCGAAAAATATATGCAGGGTCAGGTGACAGCAGATGTCa                                                                               <  1:653406/143‑1 (MQ=255)                                                         ttttACACCTTTTCCTCCCCGTCAGCCTACGGCTTCTGCCCGTTTACCGCTGACGCTGATGACGCTTGATGACTGGGCGCGTGCCACCATTACTGGCGCGGACAGCGAAAAATATATGCAGGGTCAGGTGACAGCAGATGTCa                                                                               <  1:1052092/143‑1 (MQ=255)                                                         ttttACACCCTTTCCTCCCCGTCAGCCTACGGCTTCTGCCCGTTTACCGCTGACGCTGATGACGCTTGATGACTGGGCGCGTGCCACCATTACTGGCGCGGACAGCGAAAAATATATGCAGGGTCAGGTGACAGCAGATGTCa                                                                               <  2:162524/143‑1 (MQ=255)                                                             acacCTTTTCCTCCCCGTCAGCCTACGGCTTCTGCCCGTTTACCGCTGACGCTGATGACGCTTGATGACTGGGCGCGTGCCACCATTACTGGCGCGGACAGCGAAAAATATATGCAGGGTCAGGTGACAGCAGATGTCAGCCa                                                                           <  1:690270/143‑1 (MQ=255)                                                             acacCTTTTCCTCCCCGTCAGCCTACGGCTTCTGCCCGTTTACCGCTGACGCTGATGACGCTTGATGACTGGGCGCGTGCCACCATTACTGGCGCGGACAGCG‑AAAATATATGCAGGGTCAGGTGACAGCAGATGTCAGCCAg                                                                          >  2:119080/1‑143 (MQ=255)                                                                  ttttCCTCCCCGTCAGCCTACGGCTTCTGCCCGTTTACCGCTGACGCTGATGACGCTTGATGACTGGGCGCGTGCCACCATTACTGGCGCGGACAGCGAAAAATATATGCAGGGTCAGGTGACAGCAGATGTCAGCCAGATgg                                                                      >  1:600939/1‑143 (MQ=255)                                                                  ttttCCTCCCCGGCAGCCTACGGCTTCTGCCCGTTTACCGCTGACGCTGATGATGCTTGATGACTGGGCGCGTGCCACCATTACTGGCGCGGACAGCGAAAAATATATGCAGGGTCAGGTGACAGCAGATGTCAGCCAGATgg                                                                      <  1:1146952/143‑1 (MQ=255)                                                                    ttCCTCCCCGTCAGCCTACGGCTTCGGCCCGTTTACCGCTGACGCGGATGACGCTTGATGACTGGGCGCGTGCCACCATTACTGGCGCGGACAGCGAAAAATATATGCAGGGTCAGGTGACAGCAGATGTCAGCCAGATGGCa                                                                    <  1:470395/143‑1 (MQ=255)                                                                    ggcctccCGGGCAGCCTACGGGTGCCGCCCGTTTACCGCTGACGCTGATGACGCTTGATGACTGGGCGCGTGCCACCATTACTGGCGCGGACAGCGAAAAATATATGCAGGGTCAGGTGACAGCAGATGTCAGCCAGATGGCa                                                                    <  2:172752/141‑1 (MQ=255)                                                                        tccCCGTCAGCCTACGGCCTCTGCCCGTTTACCGCTGACGCTGATGACGCTTGATGACTGGGCGCGTGCCACCATTACTGGCGCGGACAGCGAAAAATATATGCAGGGTCAGGTGACAGCAGATGTCAGCCAGATGGCagaag                                                                <  1:968497/143‑1 (MQ=255)                                                                          cccGTCAGCCTACGGCTTCTGCCCGTTTACCGCTGACGCTGATGACGCTTGATGACTGGGCGCGTGCCACCATTACTGGCGCGGACAGCGAAAAATATATGCAGGGTCAGGTGACAGCAGATGTCAGCCAGATGGCAGAAGAt                                                              <  1:1219347/143‑1 (MQ=255)                                                                           ccGTTAGCCTGCGGCTTCTGCCCGTTTACCGCTGACGCTGGTGGCGCTTGATGACTGGGCGCGTGCCACCATTACTGGCGGGGACAGCGAAAAATATATGCAGGGTCAGGTGACAGCAGATGTCAGCCAGATGGCAGAAGATc                                                             <  2:396651/143‑1 (MQ=255)                                                                           ccGTCAGCCTACGGCTTCTGCCCTTTTACCGCTGACGCTTATGACGCTTGATGACTGGGCGCTTGCCACCATTACTGTCGCGGACAGCGAAAAATATATGCAGTGTCAGGTGACAGCAGATGTCAGCCAGATGGCAGAAGATc                                                             <  2:287966/143‑1 (MQ=255)                                                                           ccGTCAGCCTACGGCTTCTGCCCGTTTCCCGCTGACGCTGATGACGCTTGATGACTGGGCGCGTGCCACCATTACTGGCGCGGACAGCGAAAAATATATGCAGGGTCAGGTGACAGCAGATGTCAGCCAGATGGCAGAAGATc                                                             <  1:424179/143‑1 (MQ=255)                                                                             gTCAGCCTACGGCTTCTGCCCGTTTACCGCTGACGCTGATGACGCTTGATGACTGGGCGCGTGCCACCATTACTGGCGCGGACAGCGAAAAATATATGCAGGGTCAGGTGACAGCAGATGTCAGCCAGATGGCAGAAGATCAg                                                           >  2:883899/1‑143 (MQ=255)                                                                             gTCAGCCTACGGCTTCTGCCCGTTTAACGCTGACGCTGATGACGCTTGATGACTGGGCGCGTGCCACCATTAGTGGCGAGGACAGCGAGAAATATATGCAGGGTCAGGGGACAGCGGGTGCCAGCCAGATGGAGGAAGAGCAg                                                           >  2:209843/1‑143 (MQ=255)                                                                               cAGCCTACGGCTTCTGCCCGTTTACCGCTGACGCTGATGACGCTTGATGACTGGGCGCGTGCCACCATTACTGGCGCGGACAGCGAAAAATATATGCAGGGTCAGGTGACAGCAGATGTCAGCCAGATGGCAGAAGATCAGCa                                                         <  1:763718/143‑1 (MQ=255)                                                                                     aCGGCTTCTGCCCGTTTACCGCTGACGCTGATGACGCTTGATGACTGGGCGCGTGCCACCATTACTGGCGCGGACAGCGAAAAATATATGCAGGGTCAGGTGACAGCAGATGTCAGCCAGATGGCAGAAGATCAGCACCTGCt                                                   >  1:1008653/1‑143 (MQ=255)                                                                                     aCGGCTTCTGCCCGTTTACCGCTGACGCTGATGACGCTTGATGACTGGGCGCGTGCCACCATTACTGGCGCGGACAGCGAAAAATATATGCAGGGTCAGGTGACAGCAGATGTCAGCCAGATGGCAGAAGAACAGCACCTGCt                                                   >  2:297536/1‑143 (MQ=255)                                                                                        gCTTCTGCCCGTTTACCGCTGACGCTGATGACGCTTGATGACTGGGCGCGTGCCACCATTACTGGCGCGGACAGCGAAAAATATATGCAGGGTCAGGTGACAGCAGATGTCAGCCAGATGGCAGAAGATCAGCACCTGCTcgc                                                >  1:322222/1‑143 (MQ=255)                                                                                          tctttcgcGTTTACTGCTGATGCTTTTGTCGCTTGAGTACTGGGCGCGTGCCACCATTACTGGCGCGGACAGCGAAAAATATATGGAGGGTCAGGTGACAGCAGATGTCAGCCAGATGGCAGAAGATCAGCACCTGCTcgccg                                              <  2:86535/136‑1 (MQ=255)                                                                                                    gtACCGCTGACGCCGATGACGCTCGAAGACTGGGCGCTTGCCACCACTACGGGCGCGGACAGCGAAAAAGATATGCAGGGTCAGGTGACAGCAGATGTCAGCCAGATGGCAGAAGATCAGCACCTGCTCGCCGCCCATTGCGa                                    <  2:528421/142‑1 (MQ=255)                                                                                                        cgcggacgctgaTGACGCTTGATGACTGGGCGCGTGCCACCATTACTGGCGCGGACAGCGAAAAATATATGCAGGGTCAGGTGACAGCAGATGTCAGCCAGATGGCAGAAGATCAGCACCTGCTCGCCGCCCATTGCGACGcc                                <  1:911319/143‑1 (MQ=255)                                                                                                            gatgctgaTGACGCGGGGTGACTGTGCGCGTGTCACCAGTACTGGCCCGGATAGCGAAAAATATATGCAGTGTCAGGTGACAGCAGAGGTCAGCCAGATGGCAGAAGATCAGCACCTGCTCGCCGCCCATTGCGACGCCAAAg                            <  2:248107/140‑1 (MQ=255)                                                                                                               gctgaTGACGCTTGATGACTGGGCGCGTGCCACCATTACTGGCGCGGACAGCGAAAAATATATGCAGGGTCAGGTGACAGCAGATGTCAGCCAGATGGCAGAAGATCAGCACCTGCTCGCCGCCGAGTGCGACGCCAAAGGTa                         >  1:157758/1‑143 (MQ=255)                                                                                                               gctgaTGACGCTTGATGACTGGGCGCGTGCCACCATTACTGGCGCGGACAGCGAAAAATATATGCAGGGTCAGGTGACAGCAGATGTCAGCCAGATGGCAGAAGATCAGCACCTGCTCGCCGCCCATTGCGACGCCAAAGGTa                         >  1:886121/1‑143 (MQ=255)                                                                                                                    tgaCGCTTGATGACTGGGCGCGTGCCACCATTACTGGCGCGGACAGCGAAAAATATATGCAGGGTCAGGTGACAGCAGATGTCAGCCAGATGGCAGAAGATCAGCACCTGCTCGCCGCCCATTGCGACGCCAAAGGTAAAAtg                    >  1:1005233/1‑143 (MQ=255)                                                                                                                     gaCGCTTGATGACTGGGCGCGTGCCACCATTACTGGCGCGGACAGCGAAAAATATATGCAGGGTCAGGTGACAGCAGATGTCAGCCAGATGGCAGAAGATCAGCACCTGCGCGCCGCGCATTGCGACGGCGAAGGGAAAAtgt                   >  2:591317/1‑143 (MQ=255)                                                                                                                     gaCGCTTGATGACTGGACGCGTGCCACCATTACTGGCGCGGACAGCGAAAAATATATGCAGGGTCAGGTGACAGCAGATGTCAGCCAGATGGCAGAAGATCAGCACCTGCTCGCCGCCCATTGCGACGCCCAAGGTAAACtgg                   >  2:757216/1‑142 (MQ=255)                                                                                                                        gCTTGATGACTGGGCGCGTGCCACCATTACTGGCGCGGACAGCGAAACATATATGCAGGGTCAGGTGACAGCAGATGTCAGCCAGATGGCAGAAGATCAGCACCTGCTCGCCGCCCATCGCGACCCCAAAGGCAACATGTGGa                >  2:686099/1‑143 (MQ=255)                                                                                                                        gCTTGATGACTGGGCGCGTGCCACCATTACTGGCGCGGACAGCGAAAAATATATGCAGGGTCAGGTGACAGCAGATGTCATCCAGATGGCAGAAGATCAGCACCTGCTCGCCGCCCATTGCGACGCCAAAGGTAAAATGTGGa                >  1:460958/1‑143 (MQ=255)                                                                                                                        gCTTGATGACTGGGCGCGTGCCACCATTACTGGCGCGGACAGCGAAAAATATATGCAGGGTCAGGTGACAGCAGATGTCAGCCAGATGGCAGAAGATCAGCACCTGCTCGCCGCCCATTGCGACGCCAAAGGTAAAATGTGGa                >  1:666771/1‑143 (MQ=255)                                                                                                                         cTTGATGACTGGGCGCGTGCCACCATTACTGGCGCGGACAGCGAAAAATATATGCAGGGTCAGGTGACAGCAGATGTCAGCCAGATGGCAGAAGATCAGCACCTGCTCGCCGCCCATTGCGACGCCAAAGGTAAAATGTGGAg               <  1:363168/143‑1 (MQ=255)                                                                                                                             atgaCTGGGCGCGTGCCACCATTACTGGCGCGGACAGCGAAAAATATATGCAGGGTCAGGTGACAGCAGATGTCAGCCAGATGGCAGAAGATCAGCACCTGCTCGCCGCCCATTGCGACGCCAAAGGTAAAATGTGGAGCAAt           >  1:482652/1‑143 (MQ=255)                                                                                                                                aCTGGGCGCGTGCCACCATTACTGGCGCGGACAGCGAAAAATATATGCAGGGTCAGGTGACAGCAGATGTCAGCCAGATGGCAGAAGATCAGCACCTGCTCGCCGCCCATTGCGAc                                   <  2:360368/116‑1 (MQ=255)                                                                                                                                aCTGGGCGCGTGCCACCATTACTGGCGCGGACAGCGAAAAATATATGCAGGGTCAGGTGACAGCAGATGTCAGCCAGATGGCAGAAGATCAGCACCTGCTCGCCGCCCATTGCGAc                                   >  1:360368/1‑116 (MQ=255)                                                                                                                                aCTGGGCGCGTGCCACCATTACTGGCGCGGACAGCGAAAAATATATGCAGGGTCAGGTGACAGCAGATGTCAGCCAGATGGCAGAAGATCAGCACCTGCTCGCCGCCCATTGCGACGCCAAAGGTAAAATGTGGAGCAATTTa        >  1:419539/1‑143 (MQ=255)                                                                                                                                      cgcgTGCCACCATTACTGGCGCGGACAGCGAAAAATATATGCAGGGTCAGGTGACAGCAGATGTCAGCCAGATGGCAGAAGATCAGCACCTGCTCGCCGCCCATTGCGACGCCAAAGGTAAAATGTGGAGCAATCTCCGTCTg  >  2:161498/1‑143 (MQ=255)                                                                                                                                         |                                                                                                                                            CTCTTTTACCATTAGTCATTAATACGCCGTTAAGCAACTCAGGACTCTATTATGGCTTTTACACCTTTTCCTCCCCGTCAGCCTACGGCTTCTGCCCGTTTACCGCTGACGCTGATGACGCTTGATGACTGGGCGCTTGCCACCATTACTGGCGCGGACAGCGAAAAATATATGCAGGGTCAGGTGACAGCAGATGTCAGCCAGATGGCAGAAGATCAGCACCTGCTCGCCGCCCATTGCGACGCCAAAGGTAAAATGTGGAGCAATTTACGTCTG  >  NC_000913/3041262‑3041537

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑