| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 1,934,850 | C→A | E489* (GAG→TAG) | zwf ← | NADP(+)‑dependent glucose‑6‑phosphate dehydrogenase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 1,934,850 | 0 | C | A | 97.9% | 137.1 / NA | 49 | E489* (GAG→TAG) | zwf | NADP(+)‑dependent glucose‑6‑phosphate dehydrogenase |
| Reads supporting (aligned to +/- strand): ref base C (0/1); new base A (19/28); total (19/30) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
TTTCAGTCAGTGTAATAAAAAAAGCCTCGTGGGTGAATCCGCACGAGGCCTGAAAGTGTAAAAATTGTTCTACAATCTGCGCAAGATCATGTTACCGGTAAAATAACCATAAAGGATAAGCGCAGATATTACTCAAACTCATTCCAGGAACGACCATCACGGGTAATCATCGCCACCGAGGCAACGGGTCCCCAGGTTCCGGCCTGATACGGTTTCGGCGCATCATTGTCCATCGCCCACGCCTCAGTAATGGAGTCTACCCATTTCCAGGCTTCTTC > NC_000913/1934711‑1934988 | attCAGCAAGTGTAATAAAAAAAGCCTCGTGGGTGAATCCGCACGCGGCCTGAAATTGCAAAAATTGTTCTACAATCTGCGCAAGATCATGTTACCGGTAAAATAACCATAAAGGATAAGCGCAGATATTACTCAAACTAAtt < 1:470693/142‑1 (MQ=255)agtcaatcagGTTAATAAAAAAAGCGTCGTGGGTTAATCCGCACGAGGCCTGAAAGTATAAAAATTGTTCTACAATCTGCGCAAGATCATGTTACCGGTAAAATAACCATAAAGGATAAGCGCAGATATTACTCAAACTAAtt < 2:206147/141‑1 (MQ=255) ttCAGTCAGTGTAATAAAAAAAGCCTCGTGTGTGAATCTGCACGAGGCCTGAAAGTGTAAAAATTGTTCTACTATCTGCGCAAGATCATGTTACCCGTAAAATAACCATAAAGGATAAGCGCAGATATTACTCAAACTAATTc < 2:675305/143‑1 (MQ=255) aaggcagtGTAATAAAAAAAGCCTCGTGGGTGAATCCGCACGAGGCCTGAAAGTGTAAAAATTGTTCTACAATCTGCGCAAGATCATGTTACCGGTAAAATAACCATAAAGGATAAGCGCAGATATTACTCAAACTAATTCCa < 2:920541/139‑1 (MQ=255) agtGTAATAAAAAAAGCCTCGTGGGTGAATCCGCACGAGGCCTGAAAGTGTAAAAATTGTTCTACAATCTGCGCAAGATCATGTTACCGGTAAAATAACCATAAAGGATAAGCGCAGATATTACTCAAACTAATTCCAGGAAc < 2:362035/143‑1 (MQ=255) tgtAATACAAAAAGCCTCGTGGGTGAATCCGCACGAGGCCTGAAAGTGTAAAAATTGTTCTACAATCTGCGCAAGATCATGTTACCGGTAAAATAACCATAAAGGATAAGCGCAGATATTACTCAAACTAATTCCAGGAACGa < 2:952308/143‑1 (MQ=255) gtAATAAAAAAAGCCTCGTGGGTGAATCCGCACGAGGCCTGAAAGTGTAAAAATTGTTCTACAATCTGCGCAAGATCATGTTACCGGTAAAATAACCATAAAGGATAAGCGCAGATATTACTCAAACTAATTCCAGGAACGAc < 1:1219960/143‑1 (MQ=255) taagaaAAAAAGCCTCGTGGGTGAATCCGCACGAGGCCTGAAAGTGTAAAAATTGTTCTACAATCTGCGCAAGATCATGTTACCGGTAAAATAACCATAAAGGATAAGCGCAGATATTACTCAAACTAATTCCAGGAACGAcc < 2:933995/143‑1 (MQ=255) ataaAAAAAGCCTCGTGGGTGAATCCGCCCGAGGCCTGAAAGTGTCAAAATTGTTCTACAATCTGCGCACGATCCTGTTACCGGTAAAATAACCATCACGGATAAGAGCAGCTATTTCTAAAACTAATACAGGGAACCAcaga > 2:656095/1‑140 (MQ=255) ataaAAAAAGCCTCGTGGGTGAATCCGCACGAGGACTGAAAGTGTAAAAGTTGTTCTACAATCTGCGCAAGATCATGTTACCGGTAAAATAACCATAAAGGATAAGCGCAGATATTACTCAAACTAATTCCAGGAACGACCAt > 1:639885/1‑143 (MQ=255) aaaaaGCCTCGTGGGTGAATCCGCACGAGGCCTGAAAGTGTAAAAATTGTTCTACAATCTGCGCACGATCATGTTACCGGTAAACTAACCATAAAGGATAAGCGCAGATATGACTCAAACTAATTCCAGGAACGACCacaacg > 2:905033/1‑138 (MQ=255) aaaaaGCCTCGTGGGTGAATCCGCACGAGGCCTGAAAGTGTAAAAATTGTTCTACAATCTGCGCAAGATCATGTTACCGGTAAAATAACCATAAAGGATAAGCGCAGATATTACTCAAACTAATTCCAGGAACGACCATCACg < 1:778810/143‑1 (MQ=255) aatgCCCCGTGGGTGCATCCTCCAGATGCCTGAAAGTGTAAAAATTGTTCTACAATCTGCGCAAGATCATGTTACCGGTAAAATAACCATAAAGGACAAGCGCAGATATTACTCAAACTAATTCCAGGAACGACCATCACggg < 2:462231/140‑1 (MQ=255) aaaGCCTCGTGGGTGAATCCGCACGAGGCCTGAAAGTGTAAAAATTGTTCTACAATCTGCGCAAGATCATGTTACCGGTAAAATAACCATAAAGGATAAGCGCAGATATTACTCAAACTAATTCCAGGAACGACCATCACggg > 1:1011799/1‑143 (MQ=255) aaaGCCTCGTGGGTGAATCCGCACGAGGCCTGAAAGTGTAAAAATTGTTCTACAATCTGCGCAAGATCATGTTACCGGTAAAATAACCATAAAGGATAAGCGCAGATATTACTCAAACTAATTCCAGGAACGACCATCACggg < 1:1009539/143‑1 (MQ=255) gCCTCGTGGGTGAATCCGCACGATGCCTTATATTGTCGAAATTATTCTACAATCTGCGCAAGATCATGTTACCGGTAAAATAACCATAAAGGATAAGCGCAGAGATTACTCAAACTAATTCCAGGAACGACCATCACGGGTaa < 2:544073/143‑1 (MQ=255) gCCTCGTGGGTGAATCCGCACGAGGCCTGAAAGTGTAGAAATTGTTCTACAATCTGCGCAAGATCATGTTACCGGTAAAATAACCATAAAGGATAAGCGCAGATATTACTCAAACTAATTCCAGGAACGACCATCACGGGTaa < 2:720431/143‑1 (MQ=255) cGTGGGTGAATCCGCACGAGGCCTGAAAGTGTAAAAATTGTTCTACAATCTGCGCAAGATCATGTTACCGGTAAAATAACCATAAAGGATAAGCGCAGATATTACTCAAACTAATTCCAGGAACGACCATCACGGGTAatcat > 1:759806/1‑143 (MQ=255) gTGGGTGAATCCGCACGAGGCCTGAAAGTGTAAAAATTGTTCTACAATCTGCGCAAGATCATGTTACCGGTAAAATAACCATAAAGGATAAGCGCAGATATTACTCAAACTAATTCCAGGAACGACCATCACGGGTAatcatc > 1:836954/1‑143 (MQ=255) gTGGGTGAATCCCCACGAGGCCTGAAAGTGTAAAAATTGTTCTACAATCTGCGCAAGATCATGTTACCGGTAAAATAACCATAAAGGATAAGCGCAGATATTACTCAAACTAATTCCAGGAACGACCATCACGGGTAatcatc < 1:388824/143‑1 (MQ=255) gTGAATCCGCACGCGGCCTGGAAGTGTAAAAATTGTTCTACAATCTGCGCAAGATCATGTTACCGGTAAAACAACCATAAAGGATAAGCGCAGATATTACTCAAACTAATTCCAGGAACGACCATCAAGGGGACTCATCGCCa > 2:779431/1‑143 (MQ=255) gTGAATCCGCACGAGGCCTGAAAGTGTAAAAATTGTTCTACAATCTGCGCAAGACCATGTTACCGGTAAAATAACCATAAAGGATAAGCGCAGATATTACTCAAACTAATTCCAGGAACGACCATCACGGGTAATCATCGCCa > 2:597158/1‑143 (MQ=255) tGAATCCGCACGAGGCCTGAAAGTGTAAAAATTGTTCTACAATCTGCGCAAGATCATGTTACCGGTAAAATAACCATAAAGGATAAGCGCAGATATTACTCAAACTAATTCCAGGAACGACCATCACGGGTAATCATCGCCAc < 1:1138705/143‑1 (MQ=255) tggCACGAGGCGTGAAAGTGTGCACATTGTTGGACGATCTGCGCAAGATCATGTGACCGGTAAAATAACCATAAAGGATAAGCGCAGATATTACTCAAACTAATTCCAGGAGCGACCATCACGGGTAATCATCGCCACCGAgg < 2:425205/141‑1 (MQ=255) cACGAGGCCTGAAAGTGTAAAAATTGTTCTACAATCTGCGCAAGATCATGTTACCGGTAAAATAACCATAAAGGATAAGCGCAGATATTACTCAAACTAATTCCAGGAACGACCATCACGGGTAATCATCGCCACCGAGGCaa < 1:1127491/143‑1 (MQ=255) gCCTGGAAGTGTAAAAATTGTTCTACAATCTGCGCAAGACCATGTTACCGGTAAAATAACCATAAACGATAAGCGCAGATATTACTCAAACTAATTCCAGGAACGACCATCACGGCTAATCATCGCCACCGAGGCAACggcgc > 2:949975/1‑140 (MQ=255) tGAAAGTGTAAAAATTGTTCTACAATCTGCGCAAGATCATGTTACCGGTAAAATAACCATAAAGGATAAGCGCAGATATTACTCAAACTAATTCCAGGAGCGGCCATCACGGGTACTCATCGCCACCCAGGCAACGCGCcccc > 2:366391/1‑143 (MQ=255) gAAAGTGTAAAAATTGTTCTACAATCTGCGCAAGATCATGTTACCGGTAAAATAACCATAAAGGATAAGCGCAGATATTACTCAAACTAATTCCAGGAACGACCATCACGGGTAATCATCGCCACCGAGGCAACGGGTCCCCa < 2:1168664/143‑1 (MQ=255) aaaGTGTAAAAATTGTTCTACAATCTGCGCAAGATCATGTTACCGGTAAAATCACCATAAAGGAGAAGCGCCGATATCACCCAAACTAATCCCAGGAACGACCATCACGGGTAAGCACCCCCCCCGCGACAAGGGGTcccccg > 2:481090/1‑141 (MQ=255) gtAAAAATTGTTCTACAATCTGCGCAAGATCATGTTACCGGTAAAATAACCATAAAGGATAAGCGCAGATATTACTCAAACTAATTCCAGGAACGACCATCACGGGTAATCATCGCCACCGGGGCAACGGGACCCCAGATTcc > 1:1020332/1‑143 (MQ=255) tAAAAATTGTTCTACAATCTGCGCAAGATCATGTTACCGGTAAAATAACCATAAAGGATAAGCGCAGATATTACTCAAACTAATTCCAGGAACGACCATCACGGGTAATCATCGCCACCGAGGCAACGGGTCCCCAGGTTCCg < 2:579117/143‑1 (MQ=255) agaaaTTGTTCGACAAGCTGGGCAAGATCATGTTACCGGTAAAATAACCATAAAGGATAAGCGCAGATATTACTCAAACTAATTCCAGGAACGACCATCGCGGGTAATCATCGCCACCGAGGCAACGGGTCCCCAGGTTCCgg < 2:811791/141‑1 (MQ=255) aaTTGTTCTACAATCTGCGCAAGATCATGTTACCGGTAAAATAACCATAAAGGATAAGCGCAGATATTACTCAAACTAATTCCAGGAACGACCATCACGGGTAATCATCGCCACCGAGGCAACGGGTCCCCAGGTTCCGGCCt < 1:1160827/143‑1 (MQ=255) tACAATCTGCGCAAGATCATGTTACCGGTAAAATAACCATAAAGGATAAGCGCAGATATTACTCAAACTAATTCCAGGAACGACCATCACGGGTAATCATCGCCACCGAGGCAACGGGTCCCCAGGTTCCGGCCTGAGACGGt > 1:655753/1‑143 (MQ=255) aTCATGTTACCGGTAAAATAACCATAAAGGATAAGCGCAGATATTACTCAAACTAATTCCAGGAACGACCATCACGGGTAATCATCGCCACCGAGGCAACGGGTCCCCAGGTTCCGGCCTGATACGGTTTCGGCGCCTCATTg > 1:724101/1‑143 (MQ=255) tGTTACCGGTAAAATAACCATAAATGATAAGCGCAGAGATTACACAAACTAATTCCAGGAACGACCATCACGGGTAATCATCGCCACCGAGGCAACGGGTCCCCAGGTTCCGGCCTGATACGGTTTCGGCGCATCATTGTCCa < 1:381342/143‑1 (MQ=255) gTTACCGGTAAATTTATCATAAGGGATAAGCGCAGATATTACTCAAACTCATTCCAGGAACGACCATCACGGGGAATCATCGCCACCGAGGCAACGGGTCCCCAGGTTCCGGCCTGAGACGGTTTCGGCGCATCATTGTCCAt < 2:685767/143‑1 (MQ=255) gTTACCGGTAAAATAACCATAAAGGATAAGCGCAGATATTACTCAAACTAATTCCAGGAACGACCATCACGGGTAATCATCGCCACCGAGGCAACGGGCCACCAGGATCCGGCCTGATACGCTTTCGGCGCATCATTGTCCAt > 2:375117/1‑143 (MQ=255) ttACCGGTAAAATAACCATAAAGGATAAGCGCAGATATTACTCAAACTAATTCCAGGAACGACCATCACGGGTAATCATCGCCACCGAGGCAACGGGTCCCCAGGTTCCGGCCTGATACGGCTTCGGCGCATCATTGTCCATc > 1:1240884/1‑143 (MQ=255) ccGGTAAAATAAACATAAAGGATAAGCGCAGATATAACTCAAACGAATTCCAGGAACGACCATCACGGGTAATCATCGCCACCGAGGCAACGGGTCCCCAGGTTCCGGCCTGATACGGTTTCGGCGCATCATTGTCCATCGcc < 1:248202/143‑1 (MQ=255) ggTAAAATAACCATAAAGGATAAGCGCAGATATTACTCAAACTAATTCCAGGAACGACCATCACGGGTAATCATCGCCACAGACTTTTCTTTTCCCCAGGTTCCGGCCTGATACGGTTTCGGCGCATCATTGTCCATCGCCCa > 1:1232881/1‑143 (MQ=255) gTAAAATAACCATAAAGGATAAGCGCAGATATTAATCAAACTAATTCCAGGAACGACCATCACGGGTAATCATCGCCACCGAGGCAACGGGTCCCCAGGTTCCGGCCTGATACGGTTTCGGCGCATCATTGTCCATCGCCCAc < 1:331906/143‑1 (MQ=255) agaaCCATAAAGGAGAAGCGCAGATATTACTCAAACTAATTCCAGGAACGACCATCACGGGTAATCATCGCCACCGAGGCAACGGGTCCCCAGGTTCCGGCCTGATACGGTTTCGGCGCATCATTGTCCATCGCCCACGCCTc < 2:444877/141‑1 (MQ=255) gATAAGCGCAGATATTACTCAAACTAATTCCAGGAACGACCATCACGGGTAATCATCGCCACCGAGGCAACGGGTCCCCAGGTTCCGGCCTGATACGGTTTCGGCGCATCATCGTCCATGGCCCACGCCTCAGTCATGGAGTc > 1:208832/1‑143 (MQ=255) ctaatACGCAGACTAACTCCAGGAACGACCATCACGGGTAATCATCGCCACCGAGGCAACGGGTCCCCAGGTTCCGGCCTGATACGGTTTCGGCGCATCATTGTCCATCGCCCACGCCTCAGTAATGGAGTCTACCCATTTcc < 1:1029927/139‑1 (MQ=255) ttACTCAAACTATTGCCGGGAACGACCATCACGGGTAATCATCGCCACCGAGGCAACGGGTCCCCAGGTTCCGGCCTGATACGGTTTCGGCGCATCATTGTCCATCGCCCACGCCTCAGTAATGGAGTCTACCCATTTCCAgg < 2:530599/143‑1 (MQ=255) ttACTCAAACTAATTCCAGGAACGACCATCACGGGTAATCATCGCCACCGAGGCAACGGGTCCCCAGGTTCCGGCCTGATACGGTTTCGGCGCATCATTGTCCATCGCCCACGCCTCAGTAATGGAGTCTACCCATTTCCAgg > 1:986749/1‑143 (MQ=255) aaaCTGATTCCAGGAACGACCATCACGGGTAATCATCGCCACCGAGGCAACGGGTCCCCAGGTTCCGGCCTGATACGGTTTCGGCGCATCATTGTCCATCGCCCACGCCTCAGTAATGGAGTCTACCCATTTCCAGGcttctt < 2:914273/143‑1 (MQ=255) aaCTAAGTCCAGGACCGAGCATCACGGGTAAGCACCGCCACCGAGGCAACGGGTCCCCAGGTTCCGGCCTGATACGGTTTCGGCGCATCATTGTCCATCGCCCACGCCTCAGTAATGGAGTCTACCCATTTCCAGGCttcttc < 2:170911/143‑1 (MQ=255) | TTTCAGTCAGTGTAATAAAAAAAGCCTCGTGGGTGAATCCGCACGAGGCCTGAAAGTGTAAAAATTGTTCTACAATCTGCGCAAGATCATGTTACCGGTAAAATAACCATAAAGGATAAGCGCAGATATTACTCAAACTCATTCCAGGAACGACCATCACGGGTAATCATCGCCACCGAGGCAACGGGTCCCCAGGTTCCGGCCTGATACGGTTTCGGCGCATCATTGTCCATCGCCCACGCCTCAGTAATGGAGTCTACCCATTTCCAGGCTTCTTC > NC_000913/1934711‑1934988 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |