| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 125,085 | 2 bp→CA | coding (2069‑2070/2664 nt) | aceE → | pyruvate dehydrogenase E1 component |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 125,085 | 0 | T | C | 98.0% | 146.2 / NA | 50 | I690T (ATC→ACA) | aceE | pyruvate dehydrogenase E1 component |
| Reads supporting (aligned to +/- strand): ref base T (0/0); major base C (27/22); minor base G (0/1); total (27/23) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.60e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
| * | NC_000913 | 125,086 | 0 | C | A | 98.0% | 137.4 / NA | 50 | I690T (ATC→ACA) | aceE | pyruvate dehydrogenase E1 component |
| Reads supporting (aligned to +/- strand): ref base C (1/0); new base A (26/22); total (27/23) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.99e-01 | |||||||||||
TCACAGCCACATTCAGTCGCTGACTATCCCGAACTGTATCTCTTACGACCCGGCTTACGCTTACGAAGTTGCTGTCATCATGCATGACGGTCTGGAGCGTATGTACGGTGAAAAACAAGAGAACGTTTACTACTACATCACTACGCTGAACGAAAACTACCACATGCCGGCAATGCCGGAAGGTGCTGAGGAAGGTATCCGTAAAGGTATCTACAAACTCGAAACTATTGAAGGTAGCAAAGGTAAAGTTCAGCTGCTCGGCTCCGGTTCTATC > NC_000913/124948‑125221 || tCACAGCCACATTCAGTCGCTGACTATCCCGAACTGTATCTCTTACGACCCGGCTTACGCTTACGAAGTTGCTGTCATCATGCATGACGGTCTGGAGCGTATGTACGGTGAAAAACAAGAGAACGTTTACTACTACACAACTa > 1:257681/1‑143 (MQ=255) aGCCACATTCAGTCGCTGACTATCCCGAACTGTATCTCTTACGACCCGGCTTACGCTTAAGAAGTGGCTGTCCTCATGCATGACGGTCTGGAGCGTATGTACGGTGAAAAACAAGAGAACGTTTACTACTACACAACTACGcg > 1:368541/1‑142 (MQ=255) ccACATTCAGTCGCTGACTATCCCGAACTGTATCTCTTACGACCCGGCTTACGCTTACGAAGTTGCTGTCATCATGCATGACGGTCTGGAGCGTATGTACGGTGAAAAACAAGAGAACGTTTACTACTACACAACTACGCTGa < 1:807265/143‑1 (MQ=255) cacaTTCAGTCGCTGACTATCCCGAACTGTATCTCTTACGACCCGGCTTACGCTTACGAAGTTGCTGTCAACATGCATGACGGACTGGAGCGTATGTACGGTGAAAAACAAGAGAACGTCTAATACTAACCAACGACGCTGaa > 2:637066/1‑143 (MQ=255) acaTTCAGTCGCTGACTATCCCGAACTGTATCTCTTACGACCCGGCTTACGCTTACGAAGTTGCTGTCATCATGCATGACGGTCTGGAGCGTATGTACGGTGAAACACAAGAGAACGTTTACTCCTACACAACTACGCTGacc > 2:415231/1‑141 (MQ=255) tCGCTGACTATCCCGAACTGTATCTCTTACGACCCGGCTTACGCTTACGAAGTTGCTGTCATCATGCATGACGGTCTGGAGCGTATGTACGGTGAAAAACAAGAGAACGTTTACTACTACACAACTACGCTGAACGAAAAcaa > 2:764312/1‑141 (MQ=255) tGACTATCCCGAACTGTATCTCTTACGACCCGGCTTACGCTTACGAAGTTGCTGTCATCATGCATGACGGCCTGGAGCGTATGTACGGTGAAAGACAAGAGAACGTTTACTACTACACCACTCCGCTGAACGAAGCCTACcac > 1:479699/1‑143 (MQ=255) tCCCGAACTGTATCTCTTACGACCCGGCTTACGCTTACGAAGTTGCTGTCATCATGCATGACGGTCTGGCGCGTATGTACGGTGAAAAACAAGAGAACGTTTACTACTAAACAAGTACGCTGAACGAAAACTACGACATGCCg > 2:162462/1‑143 (MQ=255) cGAACTGTATCTCTTACGACCCGGCTTACGCTTACGAAGTTGCTGTCATCATGCATGACGGTCTGGAGCGTATGTACGGTGAAAAACAAGAGAACGTTTACTACTACACAACTACGCTGAACGAAAACTACCACATGCCGGCa < 1:433849/143‑1 (MQ=255) gTATCTCTTACGACCCGGCTTACGCTTACGAAGTTGCTGTCATCATGCATGACGGTCTGGAGCGTATGTACGGTGAAAAACAAGAGAACGTTTACTACTACACAACTACGCTGAACGAAAACTACCACATGCCGGCAATGCCg < 2:476538/143‑1 (MQ=255) tatctTACGACCCGGCTTACGCTTACGAAGTTGCTCTCATCATGCATGACGGTCTGAAGCGTATGTACCGTGAAAAACAAGAGAACGTTTACTACTACACAACTACGCTGAACGAAAACTACCACACGCCGGCAATGCCGGaa < 2:999894/141‑1 (MQ=255) ctctTACGACCCTGCTTACGCTTACGAAGTTGCTGTCATCATGCATGACGGTCTGGAGCGTATGTACGGTGAAAAACAAGAGAACGTTTACTACTACACAACTACGCTGAACGAAAACTACCACATGCCGGCAATGCCGGAAg < 1:331208/143‑1 (MQ=255) ctctTACGACCCGGCTTACGCTTCCGAACTTGCTGTCATCATGCATGACGGTCTGGAGCGTATGTCCGGTGCAAAACAAGAGCACGTTTACTACTACACAACTACGCTGCCCGAAACCTCCCCCACGCCGCCAATGCCggccg > 1:1142517/1‑140 (MQ=255) gACCCGGCTTACGCTTACGAAGTTGCTGTCATCATGCATGACGGTCTGGAGCGTATGTACGGTGAAAAACAAGAGAACGTTTACTACTACACAACTACGCTGAACGAAAACTACCACATGCCGGCAATGCCGGAAGGTGCTGa < 1:596426/143‑1 (MQ=255) cGGCTTACGCGTACGAAGTTGCTGTCATCATGCATGACGGTCTGGAGCGTATGTACGGTGAAAAACAAGAGAACGTTTACTACTACACAACTACGCTGAACGAAAACTACCACATGCCGGCAATGCCGGAAGGTGCTGAGGaa < 2:72450/143‑1 (MQ=255) gcttacgcttacGAAGTTGCTGTCAGCATGCATGACGGTCTGGAGCGGATGTACGGTGAAAAACAAGAGAACGTTTACTACTACACAACGACGCTGAACGAAAACTACCACATGCCGGCAATGCCGGAAGGTGCTGAGGAAgg < 1:1012229/143‑1 (MQ=255) ttacgcttacgAAGTTGCTGTCATCATGCATGACGGACTGGAGAGTATGTACGGTGAAAAACAAGAGAACGTTTACTACTACACAACTACGCTGAACGAAAACTACCACATGCCGGCAATGCCGGAAGGTGCTGAGGAAGGtc > 2:87528/1‑142 (MQ=255) cgcttacgAAGTTGCTGTCATCATGCATGACGGTCTGGAGCGTATGTACGGTGAAAAACAAGAGAACGTTTACTACTACACAACTACGCTGAACGAAAACTACCACATGCCGGCAATGCCGGAAGGTGCTGAGGAAGGTATcc < 1:617177/143‑1 (MQ=255) gcttacgAAATTGCTATCATAATGCATGACGATCTCGAGCGTAAGTACGGTGAAAAACAAGAGAACGTTTAGTACTGCACAACTAGGCTCAACGAAAACTAACACATGCCGGCAATGCCGGAAGGGGCTGAGGGACGTATcca > 2:177322/1‑142 (MQ=255) cgAAGTTGCTGTCATCATGCATGGCGGTCTGGAGCGTATGTACGGTGAAAAACAAGAGAACGTTTACTACTACACAACTACGCTGAACGAAAACTTCCACATGCCGGCAATGCCGGAAGGTGCTGAGGAAGGTATCCGTAAAg < 1:404060/143‑1 (MQ=255) gAAGTTGCTGTCATCATGCATGACGGTCTGGAGCGTATGTACGGTGAAAAACAAGAGAACGTTTACTACTACACAACTACGCTGAACGAAAACTACCACATGCCGGCAATGCCGGAAGGTGCTGAGGAAGGTCTCCGTAAAgg > 1:147376/1‑143 (MQ=255) gcatcatGCATGACGGTCTGGAGCGTATGTACGGTGAAGCGACGGAATGCGTGTGCTACTACACAACTACGCTGAACGAAAACTACCACATGCCGGCAATGCCGGAAGGTGCTGAGGAAGGTATCCGTAAAGGTATCTACaaa < 2:1069750/142‑1 (MQ=255) atcatGCATGACGGTCTGGAGCGTATGTACGGTGAAAAACAAGAGAACGTTTACTACTACACAACTACGCTGAACGAAAACTACCACATGCAGGAAAGTCCAGAAGGTGCTGAGGAAGGTATCCGTAAAGGTATCTACACACt > 1:520407/1‑143 (MQ=255) tcacGCATGACGGGCTGGAGCGTATGTACGGTGAAAAACAAGAGAACGTTTACTACTACACAACTACGCTGAACGAAAACTACCACATGCCGGCAATGCCGGAAGGTGCTGAGGAAGGTATCCGTAAAGGTATCTACAAACTc < 1:123827/143‑1 (MQ=255) atgcatgACGGTCTGGAGCGTATGTACGGTGAAAAACAAGAGAACGTTTACTACTACACAACTACGCTGAACGAAAACTACCACATGCCGGCAATGCCGGAAGGTGCTGAGGCAGGTATCCGTAAAGGTATCTACAAACTCGa > 1:603036/1‑143 (MQ=255) gcaggACGGTCTGGAGCGTATGTACGGTGAAAAACAAGAGAACGTTTACTACTACACAACTACGCTGAACGAAAACTACCACATGCCGGCAATGCCGGAAGGTGCTGAGGAAGGTATCCGTAAAGGTATCTACAAACTCGaaa < 1:921491/143‑1 (MQ=255) atgACGGTCTGGAGCGTATGTACGGTGAAAAACAAGAGAACGTTTACTACTACACAACTACGCTGAACGAAAACTACCACATGCCGGCAATGCCGGAAGGTGCTGAGGAAGGTATCCGTAAAGGTATCTACAAACTCGAAACt > 1:235548/1‑143 (MQ=255) gACGGTCAGGGGCGTATGTACGGTGAAAAACAAGAGAACGTTTACTACTACACAACTACGCTGAACGAAAACTACCACATGCCGGCAATGCCGGCAGGGGCTGAGGAAGGTATCCGTAACGGTATCTACAAACTCGCAACTAt > 2:613554/1‑143 (MQ=255) aCGGTCTGGAGCGTACGTACGGTGAAAAACAAGAGAACGTTTACTACTACACAACTACGCTGAACGAAAACTACCACATGCCGGCAATGCCGGAAGGTGCTGAGGAAGGTATCCGTAAAGGTATCTACAAACTCGAAACTAtt < 2:598900/143‑1 (MQ=255) cGGTCTGGAGCGTATGTACGGTGAAAAACAAGAGAACGTTTACTACTACACAACTACGCTGAACGAAAACTACCACATGCCGGCAATGCCGGAAGGTGCTGAGGAAGGTATCCGTAAAGGTATCTACAAACTCGAAACTATTg > 1:409702/1‑143 (MQ=255) cGGTCTGGAGCGTATGTACGGTGAAAAACAAGAGAACGTTTACTACTACACAACTACGCTAAACGAAAACTACCACATGCCGGCAATGCCGGAAGGTGCTGAGGAAGGTATCCGTAAAGGTATCTACAAACTCGAAACTATTg > 2:139652/1‑143 (MQ=255) cGGTCGTGAGCGTGTGTAGGGTGAAAAACAGGAGAGCGTTTACTACTGCACAACTGCGCTGAAGGGACACTCGCCCGCGCCGGCGATGCCGGAAGGTGCTGAGGAAGGTATCCGTAAAGGTATCTACAAACTCGAAACTATTg < 2:815248/143‑1 (MQ=255) tGGAGCGTATGTACGGTGAAAAACAAGAGAACGTTTACTACTACACAACTACGCTGAACGAAAACTACCACATGCCGGCAATGCCCGAAGGTGCTGAGGAAGGTATCCGTAAAGGTATCTACAAACTCGAAACTATTGAAggg > 2:475559/1‑142 (MQ=255) gAGCGTATGTACGGTGAAAAACAAGAGAACGTTTACTACTACACAACTACGCTGAACGAAAACTACCACATGCCGGCAATGCCGGAAGGTGCTGAGGAAGGTATCCGTAAAGGTATCTACAAACTCGAAACTATTGAAGGTAg > 1:783509/1‑143 (MQ=255) gCGTATGTACGGTGAAAAACAAGAGAACGTTTACTACTACACAACTACGCTGAACGAAAACTACCACATGCCGCCAACGCCGGAAGGTGCTGAGGAAGGTATCCGCAAAGGTAGCTACAACACCGAAACTATCGCAGCTAGCa > 2:1222591/1‑143 (MQ=255) gTACGGTGAAAAACAAGAGAACGTTTACTACTACACAACTACGCTGAACGAAAACTACCACATGCCGGCAATGCCGGAAGGTGCTGAGGAAGGTATCCGTAAAGGTATCTACAAACTCGAAACTATTGAAGGTAGCAAAGGTa > 2:859652/1‑143 (MQ=255) aCGGTGAAAAACAAGAGAACGTTTACTACTACACAACTACGCTGAACCAAAACCACCACATGCCGGCAACGCCGCAAGGTGCTGAGGAAGGTATCCGTAAAGGTATCTACAAACTCGAAACTATTGAAGGTAGCAAAGGTaaa < 1:87528/143‑1 (MQ=255) ggTGAAAAACAAGAGAACGTTTACTACTACACAACTACGCTGAACGAAAACTACCACATGCCGGCAATGCCGGAAGGTGCTGAGGAAGGTATCCGTAAAGGTATCTACAAACTCGAAACTATTGAAGGTAGCAAAGGTAAAGt > 1:549676/1‑143 (MQ=255) ggTGAAAAACAAGAGAACGTTTACTACTACACAACTACGCTGAACGAAAACTACCACATGCCGGCAATGCCGGAAGGTGCTGAGGAAGGTATCCGTAAAGGTATCTACAAACTCGAAACTATTGAAGGTAGCAAAGGTAAAGt > 1:1013100/1‑143 (MQ=255) tggaaaaCAAGAGAACGTGTACGACTACACAACTACGCTGGACGAAAACTACCACATGCCGGCAATGCCGGAAGGTGCTGAGGAAGGTATCCGTAAAGGTATCTACAAACTCGAAGCTATTGAAGGTAGCAAAGGTAAAGTTc < 2:939263/140‑1 (MQ=255) aaaaaCAAGCGAACGTTTACTACTACACAACTACGCTGAACGAAAACTACCACATGCCGGCAATGCCGGAAGGTGCTGAGGAAGGTATCCGTAAAGGTATCTACAAACTCGAAACTATTGAAGGTAGCAAAGGTAAAGTTCAg < 2:498667/143‑1 (MQ=255) aacaCAAGAGAACGTTTACTAGTACAGAACGACGCGGAACGAAAACTACCACATGCCGGCAATGCCGGAAGGGGCCGAGGAAGGTATCCGTAAAGGTATCTACAAACTCGAAACTATTGAAGGTAGCAAAGGTAAAGTTCAgc < 1:303545/140‑1 (MQ=255) aaCAAGAGGACGTTTACTACTACACAACTACGCTGAACGAAAACTACCACATGCCGGCAATGCCGGAAGGTGCTGAGGAAGGTATCCGTAAAGGTATCTACAAACTCGAAACTATTGAAGGTAGCAAAGGTAAAGTTCAgctg < 1:202749/143‑1 (MQ=255) aaCAAGAGAACGTTTACTACTACACAACTACGCTGAACGAAAACTACCACATGCCGGCAATGCCGGAAGGTGCTGAGGAAGGTATCCGTAAAGGTATCTACAAACTCGAAACTATGGAAGGTAGCAAAGGTAAAGttgcggcg > 2:729216/1‑137 (MQ=255) aaGAGAACGTTTACTACTACACAACTACGCTGAACGAAAACTACCACATGCCGGCAATGCCGGAAGGTGCTGAGGAAGGTATCCGTAAAGGTATCTACACACTCGAAAATATTGAAGGTAGCAAAGGTAAAGTTCAGATGCTc > 1:733440/1‑143 (MQ=255) aaGAGAACGTTTACTACTACACAACTACGCTGAACGAAAACTACCACATGCCGGCAATGCCGGAAGGTGCTGAGGAAGGTATCCGTAAAGGTATCTACAAACTCGAAACCATTGACAGTAGCAAAGATATAGCTCAgctgcgc > 2:125333/1‑141 (MQ=255) cGTTTACTACTACACAGCGACGCTGAACGTAAACTACCACATGCCGGCAATGCCGGAAGGTGCTGAGGAAGGTATCCGTAAAGGTATCTACAAACTCGAAACTATTGAAGGTAGCAAAGGTAAAGTTCAGCTGCTCGGCTCCg < 2:1224058/143‑1 (MQ=255) ttACTGCTACACTGCTACGCGGAACGAAACCTACCACATGCCGGCAATGCCGGAAGGTGCTGAGGAAGGTATCCGTAAAGGTATCTACAAACTCGAAACTATTGAAGGTAGCAAAGGTAAAGTTCAGCTGCTCGGCTCCGGtt < 2:918103/143‑1 (MQ=255) tactacACAACTACGCTGAACGAAAACTACCACATGCCGGCAATGCCGGAAGGTGCTGAGGAAGGTATCCGTAAAGGTATCTACAAACTCGAAACTATTGAAGGTAGCAAAGGTAAAGTTCAGCTGCTCGGCTCCGGTTCTAt > 2:517468/1‑143 (MQ=255) actacACAACTACGCTGAACGAAAACTACCACATGCCGGCAATGCCGGAAGGTGCTGAGGAAGGTATCCGTAAAGGTATCTACAAACTCGAAACTATTGAAGGTAGCAAAGGTAAAGTTCAGCTGCTCGGCTCCGGTTCTATc < 1:764312/143‑1 (MQ=255) || TCACAGCCACATTCAGTCGCTGACTATCCCGAACTGTATCTCTTACGACCCGGCTTACGCTTACGAAGTTGCTGTCATCATGCATGACGGTCTGGAGCGTATGTACGGTGAAAAACAAGAGAACGTTTACTACTACATCACTACGCTGAACGAAAACTACCACATGCCGGCAATGCCGGAAGGTGCTGAGGAAGGTATCCGTAAAGGTATCTACAAACTCGAAACTATTGAAGGTAGCAAAGGTAAAGTTCAGCTGCTCGGCTCCGGTTCTATC > NC_000913/124948‑125221 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |