| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_000913 | 2,173,361 | Δ2 bp | 100% | pseudogene (917‑918/1358 nt) | gatC ← | galactitol‑specific PTS enzyme IIC component |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 2,173,361 | 0 | C | . | 96.0% | 203.6 / ‑0.1 | 50 | pseudogene (918/1358 nt) | gatC | galactitol‑specific PTS enzyme IIC component |
| Reads supporting (aligned to +/- strand): ref base C (0/2); new base . (26/22); total (26/24) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.25e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
| Rejected as polymorphism: E-value score below prediction cutoff. | |||||||||||
| Rejected as polymorphism: Frequency below/above cutoff threshold. | |||||||||||
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. | |||||||||||
| Rejected as polymorphism: Polymorphic indel expands or contracts a homopolymer stretch. | |||||||||||
| * | NC_000913 | 2,173,362 | 0 | C | . | 96.0% | 203.4 / ‑1.4 | 50 | pseudogene (917/1358 nt) | gatC | galactitol‑specific PTS enzyme IIC component |
| Reads supporting (aligned to +/- strand): ref base C (0/1); new base . (26/22); total (26/24) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.69e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.99e-01 | |||||||||||
| Rejected as polymorphism: E-value score below prediction cutoff. | |||||||||||
| Rejected as polymorphism: Frequency below/above cutoff threshold. | |||||||||||
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. | |||||||||||
| Rejected as polymorphism: Polymorphic indel expands or contracts a homopolymer stretch. | |||||||||||
AGATTTCCACGATGCACGGCGACCGCCATCGCCACGAAGAAGCCGATGGTGGCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACAGCAATTAAAATGGTGAGTGGGATAAAAATCAGGCTTGCCGATACCACCCCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCCTGCTTAGCGATGGGCGTTAAACCATCCATGATGGGTTTAATCACC > NC_000913/2173219‑2173493 || aGATTTCCACGATGCACGGCGACCGCCATCGCCACGAAGAAGCCGATGGTGGCAAGATCGCCAAACGGCAGCACCTGCTTACCCGGCACACAAACAGCAATTAAAATGGTGAGTGGGATAAAAATCAGGCTTGCCGATaccac > 2:496192/1‑143 (MQ=255) gATTTCCACGATGCACGGCGACCGCCATCGCCACGAAGAAGCCGATGGTGGCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACAGCAATTAAAATGGTGAGTGGGATAAAAATCAGGCTTGCCGATaccacc > 1:149149/1‑143 (MQ=255) ccAGGATGCAGGGGGAGCGGCATCGCCACGAAGGAGCCGATGGTGGCAAGATCGCCAAATGGCAGCACCTGATGACCCGGCACACAAACAGTAATTAAAATGGTGAGTGGGATAAAAAGCAGGCGTGCCGATaccaccgccgt < 2:469521/143‑6 (MQ=255) cACGATGCACGGCGACCGCCATCGCCACGAAGAAGCCGAAGGTGGCAAGATCGCCAAACGGCAGCACCTGATTAACCGGCACACAAACAGCAATTAAAATGGTGAGTGGGATAAAAATCAGGCTTGCCGATaccaccgccgta > 1:1043476/1‑137 (MQ=255) aCGATGCACGGCGACCGCCATCGCCACGAAGAAGCCGATGGTGGCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACAGCAATTAAAATGGTGAGTGGGATAAAAATCAGGCTTGCCGATaccaccgccgtat < 1:444391/143‑8 (MQ=255) ggtGCACGGCGACCGCCATCGCCACGACGCAGCCGATGGTGGCAAGATCGCCAAACGGCAGGACCTGATTACCCTGCACACAAACAGCAATTAAAATGGTGAGTGGGATAAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGt < 2:483300/141‑1 (MQ=255) tGCACGGCGACCGCCATCGCCACGAAGAAGCCGATGGTGGCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACAGCAATTAAAATGGTGAGTGGGATAAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTcc < 1:1232413/143‑1 (MQ=255) ggCGACCGCCATCGCCACGAAGAAGCCGATGGTGGCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACAGCAATTAAAATGGAGAGTGGGATAAAACTCAGGCTTGCCGATACCA‑‑CCGCCATATGTCAcagca > 2:1027318/1‑143 (MQ=255) gCGACCGCCGTCGCCACGAAGAAGCGGATGGGGGCAAGATCGCCAATCGTCAGCACCTGATTACCCGGCACACAAACAGCAATTAAAATGGTGAGTGGGATAAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCcagcag < 2:761468/143‑1 (MQ=255) gCGACCGCCATCGCCACGAAGAAGCCGATGGTGGCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACAGCAATTAAAATGGTGAGTGGGATAAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCcagcag < 1:387774/143‑1 (MQ=255) aCCGCCATCGCCACGAAGAAGCCGATGGTGGCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCACACAAATGGCATTTAAATTGGTGAGCTGCTTTATTATCAGGCTTGCCGATACCA‑‑CCGCCGCATGTCCGAGCAGCaa > 1:238348/1‑143 (MQ=255) aCCGCCATCGCCACGAAGAAGCCGATGGTGGCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACAGCAATTAAAATGGTGAGTGGGATAAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCaa > 1:875871/1‑143 (MQ=255) ccGCCATCGCCACGAAGAAGCCGATGGTGGCAAGATCGCCAAACGGCAGCACCTGATTACCAGGAACAAAAACAGCAATTAAAATGGTGAGGGGGATACAAATCAGGGTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAAc > 2:860349/1‑143 (MQ=255) cGCCATCGCCACGAAGAAGCCGATGGTGGCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACAGCAATTAAAATGGTGAGTGGGATAAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACg > 1:652420/1‑143 (MQ=255) cGCCATCGCCACGAAGAAGCCGATGGTGGCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACAGCAATTAAAATGGTGAGTGGGATAAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACg > 1:965776/1‑143 (MQ=255) ccATCGCCACGAAGAAGCCGATGGTGGCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACAGCAATTAAAATGGTGAGTGGGATAAAAATCAGGCTTGCCGATACCA‑‑CCGCCGAAGGTCCCAGCAGCAACGcc > 2:1088469/1‑143 (MQ=255) ccATCGCCACGAAGAAGCCGATGGTGGCAAGATCGCAAACAAGCAGCACCTGATTACCCGGCACACAAACAGCAATTAAAATGGTGAGTGGGATAAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGcc < 2:1241180/143‑1 (MQ=255) aCGAAAAAGCCGATGGTGGCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACAGCAATTAAAATGGTGAGTGGGATAAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCCAg > 2:1066151/1‑143 (MQ=255) cgtagaagCCAATGGTGGCAAGATCGCCCAACGGCAGCACCTGATTACCCGTCCCACAAACAGCAATTAAAATGGTGAGGGGTATAAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAgg < 2:160389/140‑1 (MQ=255) cGAAGAAGCCGATGGTGGCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACAGCAATTAAAATGGTGAGTGGGGTGAAAATCAGGCTTGCCGATACCA‑‑CCGACGGATGTCCCAGCAGCACCGCCGGATCAAgg > 2:321993/1‑143 (MQ=255) ccGATGGTGGCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACAGCAATTAAAATGGTGAGTGGGATAAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAg < 1:438442/143‑1 (MQ=255) ccGATGGTGGCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACAGCAATTAAAATGGTGAGTGGGATAAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTACGTCCCAGCAGCAAAGCAGGATCAAGGcaagtccg > 2:204809/1‑136 (MQ=255) gATGGTGGCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACAGCAATTAAAATGGTGAGTGGGATAAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGa > 1:465945/1‑143 (MQ=255) cgggtggCAAGATCGGCAAACGGCAGCACCTGATTACCCGGCACACAAACAGCAATTAAAATGGTGAGTGGGATAAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGaa < 2:198731/141‑1 (MQ=255) aTGGTGGCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACAGCAATTAAAATGGTGAGTGGGATAAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGaa < 1:1118144/143‑1 (MQ=255) gtggCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACAGCAATTAAAATGGTGAGTGGGATCAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCCGCAACGCCGCACCACGGCCAACCACGAACTc > 2:520468/1‑143 (MQ=255) ggCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACAGCAATTAAAATGGTGAGTGGGATAAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCt > 2:191273/1‑143 (MQ=255) cAAGATCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACAGCAATTACAATAGCGAGCGGGATAAAAAACAGGCTTGCCGAAACCA‑‑CCGCCGTATAACCCAGCAAAAACGCCGGAACACAGCCAAACAGGCACtacata > 2:608053/1‑138 (MQ=255) agctggCCACACGGCAGCACCCGACTCCCCGGCACACCACCAGCAAGTAAAATGGTGAGTGGTATAAAAATCAGGCTCGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGAcc < 2:79039/138‑1 (MQ=255) aTCGCCAAACGGCAGCACCTGATGACCCGGCACACAAACAGCAATTAAAATGGTGAGTGGGATAAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGAccgc < 2:1019135/143‑1 (MQ=255) aaCGGCAGCACCTGATTACCCGGCACACAAACAGCAATTAAAATGGTGAGTGGGATAAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACtt > 1:178504/1‑143 (MQ=255) gcagcaCCTGATTACCCGGCACACAAACAGCAATTAAAATGGTGAGTGGGATAAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGcc < 2:540662/143‑1 (MQ=255) cTGATTACCCGGCACACAAACAGCAATTAAAATGGTGAGTGGGATAAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGTAGCAACGCCGGATCAAGGCCAACCAGGAACTCCTGACCGCCGAACTACGCCTGTAAAc > 2:1129797/1‑143 (MQ=255) acacaAACAGCAATTAAAATGGTGAGTGGGATAAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCCTg < 2:929274/143‑1 (MQ=255) acacaAACAGCAAATAAAACGGTGAGTGGGATAAAAATCCGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCCTg < 1:287467/143‑1 (MQ=255) aTTAAAATGGTGACTGAGATAAAAATCACCCTTGCCGATACCA‑‑CCTCCGTATGTCCCAGCAGCAACGCCGGATCAAGCCCAATCACCAACTCCTCACCGCCGAACTTCGCCTGTAAACGACTACGAGCCTGCTTACCGATggg < 2:652420/143‑1 (MQ=255) tAAAATGGTGAGTGGGATAAAAATCAGGCTTGCCGATACCA‑‑CCACCGTATGACCCAGCAGCAACGCAGGATCAAGGCCAATCAGGAACACCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCCTGCTTAGCGATGGGCg > 1:358641/1‑143 (MQ=255) aaaTGGTGAGTGGGATAAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTCCGAGCCTGCTTCGCGATGGGCGtt > 1:236966/1‑143 (MQ=255) aaaTGGTGAGTGGGATAAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCCTGCTTAGCGACCGGCGtc > 1:314198/1‑142 (MQ=255) gggTGAGTGGGATAAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCCTGCTTAGCGATGGGCGTTaaa < 1:638668/142‑1 (MQ=255) ggTGAGTGGGATAAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCCTGCTTAGCGATGGGCGTTCAAc > 1:41558/1‑143 (MQ=255) gTGAGTGGGATAAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCCTGCTTAGCGATGGGCGTTAAAcc < 1:496192/143‑1 (MQ=255) tGAGTGGGATAAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCAGACCGCCGAACTTCGCCTGTAAACGACTACGAGCCTGCTTAGCGATGGGCGTTCAAcca > 2:628908/1‑143 (MQ=255) gTGGGATAAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCCTGCTTAGCGATCGGCGGTAAAccaccc > 2:1092008/1‑143 (MQ=255) aTAAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCCTCCTTAGCGATGGGCGTTAAACCATCCatgat > 1:1065366/1‑143 (MQ=255) tAAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAATGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCCGGCTTAGCGAGGGGCGTCAAAccacccacaatg > 1:874456/1‑138 (MQ=255) tAAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGTCGAACTTCGCCTGTAAACGACTACGAGCCTGCTTAGCGATGGGCGTTAAACCATCCatgatg < 2:465945/143‑1 (MQ=255) tAAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCCTGCTTAGCGATGGGCGTTAAACCATCCatgatg > 2:892518/1‑143 (MQ=255) aaaaaTCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGATCCTGCTTAGCGATGGGCGTTAAACCATCCATGATgg < 1:826579/143‑1 (MQ=255) aaaaaGCAGACTCGCCCATACCACGA‑‑CCGTAAGTCCCAGCAGAAACGCCGGATCAAGGCCCATCGGGAAGTCGTGACCGCGGAACTGCGCCTGAAAACAACTACGTGGGTTCTTTGCGATGGGCGTTAAACCATCCATGATgg < 2:516525/143‑1 (MQ=255) aaTCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAATCGACTACGAGCCTGCTTAGCGATGGGCGTTAAACCATCCATGATGGGtt < 2:1043476/143‑1 (MQ=255) cgggCTTGCCGATGCGA‑‑CCGCCGTATGGCGCAGCAGCAACGCCGGATCAAGGCCAAGCGGGAACTCCTGGCCGCCGAACTTCGCCTGTAAACGACTACGAGCCTGCTTAGCGATGGGCGTTAAACCATCCATGATGGGTTTaa < 2:437569/141‑1 (MQ=255) cAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTGCGCCTGTAAACGACTACGAGCCTGCTTAGCGATGGGCGTTAAACCATCCATAATGGGTTTaa < 2:875871/143‑1 (MQ=255) cAGGCTTGCCGACAC‑‑CCCCGCCGTAGGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCCTGCTTAGCGATGGGCGTTAAACCATCCATGATGGGTTTaa < 2:238348/143‑1 (MQ=255) ttGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGAACGCAGAACTTCGCCTGTAAACGACTACGAGCCTGCTTAGCGATGGGCGTTAGACCAGCCATGATGGGGTTGATGAcc > 1:1145057/1‑143 (MQ=255) || AGATTTCCACGATGCACGGCGACCGCCATCGCCACGAAGAAGCCGATGGTGGCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACAGCAATTAAAATGGTGAGTGGGATAAAAATCAGGCTTGCCGATACCACCCCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCCTGCTTAGCGATGGGCGTTAAACCATCCATGATGGGTTTAATCACC > NC_000913/2173219‑2173493 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |