| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_000913 | 282,518 | G→A | 7.1% | G81S (GGC→AGC) | yagE → | CP4‑6 prophage; putative 2‑keto‑3‑deoxygluconate aldolase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 282,518 | 0 | G | A | 7.1% | 151.8 / 2.6 | 56 | G81S (GGC→AGC) | yagE | CP4‑6 prophage; putative 2‑keto‑3‑deoxygluconate aldolase |
| Reads supporting (aligned to +/- strand): ref base G (22/30); new base A (2/2); total (24/32) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.74e-01 | |||||||||||
CGACGATCTGATCAAAGCAGGCGTTGACGGCCTGTTCTTCCTGGGCAGCGGTGGCGAGTTCTCCCAGCTCGGCGCCGAAGAGCGTAAAGCCATTGCCCGCTTTGCTATCGATCATGTCGATCGTCGCGTGCCGGTGCTGATCGGCACCGGCGGCACCAACGCCCGGGAAACCATCGAACTCAGCCAGCACGCGCAGCAGGCGGGCGCGGACGGCATCGTGGTGATCAACCCCTACTACTGGAAAGTGTCGGAAGCGAACCTGATCCGCTATTTCGAGCAGGT > NC_000913/282376‑282657 | cgacgaTCTGATCAAAGCAGGCGTTGACGGCCTGTTCTTCCTGGGCAGCGGTGGCGAGTTCTCCCAGCTCGGCGCCGAAGAGCGTAAAGCCATTGCCCGCTTTGCTATCGATCATGTCGATCGTCGCGTGCCGGTGCTGATCg < 2:28202/143‑1 (MQ=255) ggcgaTCTGATCAAAGCAGGCGGGGACGGCCTGTTCTTCCTGGGCAGCGGTGGCGAGTTCTCCCAGCTCGGCGCCGAAGAGCGTAAAGCCATTGCCCGCTTTGCTATCGATCATGTCGATCGTCGCGTGCCGGTGCTGATCgg < 1:365347/141‑1 (MQ=255) tCTGATCAAAGCAGGCGTTGACGGCCTGTTCTGCCCGGGCAGCGGTGGCGGGTTCTCCCAGCGCGGCGCCGAAGAGCGTAAAGCCATTGCCCGCTTTGCTATCGATCATGTCGATCGTCGCGTGCCGGTGCCGATCAGCACcg < 2:1012798/143‑1 (MQ=255) tGATCAACGCAGGCGTTGACGGCCTGTTCTTCCTGGGCAGCGGTGGCGAGTTCTCCCAGCTCGGTGCCGAAGAGCGTAAAGCCATTGCCCGCTTTGCTATCGATCATGTCGATCGTCGCGTGCCGGTGCCGATCAGCACcggc < 2:1031339/143‑1 (MQ=255) atccgaGCAGGCGTTGGCCGCCCGTTCGTCCTGGGGCGCGGCGGCGAGTGCTCCCAGCTCGGCGCCGGAGAGCGTAAAGCCATTGCCCGCTTTGCGATCGAGCATGTCGATCGTCGCGTGCCGGTGCTGATCGGCACcggcgg < 1:97657/138‑1 (MQ=255) aTCAAAGCAGGCGTTGACGGCCTGTTCTTCCTGGGCAGCGGTGGCGAGTTCTCCCAGCTCGGCGCCGAAGAGCGTAAAGCCATTGCCCGCTTTGCTATCGATCATGTCGATCGTCGCGTGCCGGTGCTGATCGGCACcggcgg > 1:1056291/1‑143 (MQ=255) tCAAAGCAGGCGTTGACGGCCTGTTCTTCCTGGGCAGCGGTGGCGAGTTCTCCCAGCTCGGCGCGGACGAGCGTAAAGCCATTGCCCGCTTTGTTATCGATCATGTCGATCGTCGCGTGCCGGTGCTGATCGGCACCggcggc > 1:419011/1‑143 (MQ=255) cAACGCAGGCGTTGACGGCCTGTTCTTCCTGGGCAGCGGTGGCGAGTTCTCCCAGCTCGGCGCCGAAGAGCGTAAAGCCATTGCCCGCTTTGCTATCGATCATGTCGATCGTCGCGTGCCGGTGCTGATCGGCACCGGCGGCa < 2:91774/143‑1 (MQ=255) cAAAGCAGGCGTTGACGGCCTGTTCTTCCTGGGCAGCGGTGGCGAGTTCTCCCAGCTCGGCGCCGAAGAGCGTAAAGCCATTGCCCGCTTTGCTATCGATCATGTCGATCGTCGCGTGCCGGTGCTGATCGGCACCGGCGGCa > 1:774744/1‑143 (MQ=255) cAAAGCAGGCGTTGACGGCCTGTTCTTCCTGGGCAGCGGTGGCGAGTTCTCCCAGCTCGGCGCCGAAGAGCGTAAAGCCATTGCCCGCTTTGCTATCGAGCATGTCGATCGTCGCGTGCCGGTGCTGATCGGCACCGGCGGCa > 1:536641/1‑143 (MQ=255) cAGGCGTTGACGGCCTGTTCTTCCTGGGCAGCGGTGGCGAGTTCTCCCAGCTCGGCGCCGAAGAGCGTAAAGCCATTGCCCGCTTTGCTCTCGATCACGTCGATCGTAGCGTGCCGGTGCTGATCGGCACCGGCGGCACCGAc > 2:834923/1‑143 (MQ=255) cAGGCGTTGACGGCCTGTTCTTCCTGGGCAGCGGTGGCGAGTTCTCCCAGCTCGGCGCCGAAGAGCGTAAAGCCATTGCCCGCTTTGCTATCGATCATGTCGATCGTCGCGTGCCGGTGCTGATCGGCACCGGCGGCACCAAc < 1:1067529/143‑1 (MQ=255) ggtcaCGGCCTGTTCTTCCTGGGCAGCGGTGGCGAGTTCTCCCAGCTCGGCGCCGAAGAGCGTAAAGCCATTGCCCGCTTTGCTATCGATCATGTCGATCGTCGCGTGCCGGTGCTGATCGGCACCGGCGGCACCAACGCCCg < 1:606211/139‑1 (MQ=255) gTTGACGGCCTGTTCTTCCTGGGCAGCGGTGGCGAGTGCTCCCAGCTCGGCGCCGAAGAGCGTAAAGCCATTGCCCGCTTTTCTATCGATCATGTCGATCGTCGCGTGCCGGTGCTGATCGGCACCGGCGGCACCAACGCCCg < 1:480386/143‑1 (MQ=255) ggCCTGTTCTTCCTGGGCAGCGGTGGCGAGTTCTCCCAGCTCGGCGCCGAAGAGCGTAAAGCCATTGCCCGCTTTGCTATCGATCATGTCGATCGTCGCGTGCCGGTGCTGATCGGCACCGGCGGCACCAACGCCCGGGAAAc < 1:981667/143‑1 (MQ=255) ggCCTGTTCTTCCTGGGCAGCGGTGGCGAGTTCTCCCAGCTCGGCGCCGAAGAGCGTAAAGCCATTGCCCGCTTTGCTATCGATCATGTCGATCGTCGCGTGCCGGTGCTGATCGGCACCGGCGGCACCAACGCCCGGGAAAc > 1:610630/1‑143 (MQ=255) ccTGTTCTTCCTGGGCAGCGGTGGCGAGTTCTCCCAGCTCGGCGCCGAAGAGCGTAAAGCCATTGCCCGCTTTGCTATCGATCATGTCGATCGTCGCGTGCCGGTGCTGATCGGCACCGGCGGCACCAACGCCCGGGAAACCa < 1:758851/143‑1 (MQ=255) cTGTTCTTCCTGGGCAGCGGTGGCGAGTTCTCCCAGCTCGGCGCCGAAGAGCGTAAAGCCATTGCCCGCTTTGCTATCGATCATGTCGATCGTCGCGTGCCGGTGCTGATCGGCACCGGCGGCACCAACGCCCGGGAAACCAt > 1:1009441/1‑143 (MQ=255) cTGTTCTGCCTGGCCAGCGATGGCGAGTTCTCCCCGCTCGGCGCCGAAGAGCGTAAAGCCATTGCCCGCTTTGCTATCGATCATGTCGATCGTCGCGTGCCGGTGCTGATCGGCACCGGCGGCACCAACGCCCGGGAAACCAt < 2:308337/143‑1 (MQ=255) ttcttcCTGGGCAGCGGTGGCGAGTTCTCCCAGCTCGGCGCCGAAGAGCGTAAAGCCATTGCCCGCTTTGCTATCGATCATGTCGATCGTCGCGTGCCGGTGCTGATCGGCACCGGCGGCACCAACGCCCGGGAAACCATCGa < 2:664721/143‑1 (MQ=255) tcttcCTGGGCAGCGGTGGCGAGTTCTCCCAGCTCGGCGCCGAAGAGCGTAAAGCCATTGCCCGCTTTGCTATCGATCATGTCGATCGTCGCGTGCCGGTGCTGATCGGCACCGGCGGCACCAACGCCCGGGAAACCATCGaa < 1:1024484/143‑1 (MQ=255) gcttcCTGGGCAGCGGTGGCGAGTTCTCCCAGCTCGGCGCCGAAGAGCGTAAAGCCATTGCCCGCTTGTCTATCGATCATGTCGATCGTTGCGTGCCGGTGCTGATCGGCACCGGCGGCACCAACGCCCGGGAAACCATCGaa < 1:357933/142‑1 (MQ=255) cccgggCAGCGGTGGCGAGCTCACCCAGCTCGGCGCCGAAGAGCGTAAAGCCATTGCCCGCTTTGCTATCGATCATGTCGATCGTCGCGTGCCGGTGCTGATCGGCACCGGCGGCACCAACGCCCGGGAAACCATCGAACTca < 1:73402/140‑1 (MQ=255) cTGGGCAGCGGTGGCGAGTTCTCCCAGCTCGGCGCCGAAGAGCGTAAAGCCATTGCCCGCTTTGCTATCGATCATGTCGATCGTCGCGTGCCGGTGCTGATCGGCACCGGCGGCACCAACGCCCGGGAAACCATCGAACTcag < 1:952656/143‑1 (MQ=255) tGGGCAGCGGTGGCGAGTTCTCCCAGCTCGGCGCCGAAGAGCGTAAAGCCATTGCCCGCTTTGCTATCGATCATGTCGATCGTCGCGTGCCGGTGCTGATCGGCACCGGCGGCACCAACGCCCGGGAAACCATCGAACTcagc > 1:193653/1‑143 (MQ=255) ggCAGCGGTGGCGAGTTCTCCCAGCTCGGCGCCGAAGAGCGTAAAGCCATTGCCCGCTTTGCTATCGATCATGTCGATCGTCGCGTGCCGGTGCTGATCGGCACCGGCGGCACCAACGCCCGGGAAACCATCGAACTcagcca < 1:412640/143‑1 (MQ=255) gCGGTTGCGAGTTCTCCCCGCTGGGCGCCGAACAGCGTAAAGCCGCTGCCCGCTTTGCTATCGATCATGTCGATCGTCGCGTGCCGGTGCTGATCGGCACCGGCGGCACCAACGCCCGGGAAACCATCGAACTCAGCCAGCAc < 1:473859/143‑1 (MQ=255) gAGTTCTCCCAGCTCGGCGCCGAAGAGCGTAAAGCCATTGCCCGCTTTGCTATCGATCATGTCGATCGTCGCGTGCCGGTGCGGATTGGAAACGTTAACTTCAACGCCCGGGAAACCATCGAACTCCGCCCGCACGCgcagca > 1:238746/1‑143 (MQ=255) aGTTCTCCCAGCTCGGCGCCGAAGAGCGTAAAGCCATTGCCCGCTTTGCTATCGATCATGTCGATCGTCGCGTGCCGGTGCTGATCGGCACCGGCGGCACCAACGCCCGGGAAACCATCGAACTCAGCCAGCACGCGcagcag < 1:555432/143‑1 (MQ=255) aGCTCGGCGCCGAAGAGCGTAAAGCCATTGCCCGCTTTGCTATCGATCATGTCGATCGTCGCGTGCCGGTGCTGATCGGCACCGGCGGCACCAACGCCCGGGAAACCATCGAACTCAGCCAGCACGCGCAGCAGGCGGGcgcg > 1:503307/1‑143 (MQ=255) cTCGGCGCGGAAGAGCGTAAAGCCATTGCCCGCGTTGCTATCGATCATGTCGATCGTCGCGTGCCGGTGCTGATCGGCACCGGCGGCACCAACGCCCGGGAAACCATCGAACTCAGCCAGCACGCGCAGCAGGCGGGCGCGGa < 1:371931/143‑1 (MQ=255) ccGGCGCCGAAGAGCGTAAAGCCATTGCCCGCTTTGCTATCGATCATGTCGATCGTCGCGTGCCGGTGCTGATCGGCACCGGCGGCACCAACGCCCGGGAAACCATCGAACTCAGCCAGCACGCGCAGCAGGCGGGCGCGGAc < 2:361493/142‑1 (MQ=255) ggCGCCGAAGAGCGTAAAGCCATTGCCCGCTTTGCTATCGATCATGTCGATCGTCGCGTGCCGGTGCTGATCGGCACCGGCGGCACCAACGCCCGGGAAACCATCGAACTCAGCCAGCACGCGCAGCAGGCGGGCGCGGACgg < 2:1056291/143‑1 (MQ=255) cgagtgCGTATAGCCTTGGCCCGCTTTGCTATCGATCATGTCGTTCGTCGCGTGCCGGTGCTGATCGGCACCGGCGGCACCAACGCCCGTGAAACCATCGAACTCAGCCAGCACGCGCAGTAGGCGGGCGCGGACGGCATCgt < 2:1189668/138‑1 (MQ=255) aggagCGTGAGGCCATTGCCCGCTTTGCTATCGATCATGTCGATCGTCGCGTGCCGGTGCTGATCGGCACCGGCGGCACCAGCGCCCGGGAAACCATCGAACTCAGCCAGCACGCGCAGCAGGCGGGCGCGGACGGCATCgtg < 2:493233/141‑1 (MQ=255) aaGAGCGTAAAGCCATTGCCCGCTTTGCTATCGATCATGTCGATCGTCGCGTGCCGGTGCTGAACGGCACCGGCGGCACCAACGCCCGGGAAACCATCGAACTCAGCCAGCACGCGCAGCCGGCGGGCGCGGACCGCATCgtg > 2:586472/1‑143 (MQ=255) aaGAGCGTAAAGCCATTGCCCGCTTTGCTATCGATCATGTCGATCGTCGCGTGCCGGTGCTCATCGGCACCGGCGGCACCAACGCCCGGGAAACCATCGAACTCAGCCAGCACGCGCAGCAGGCGGGCGCGGACGGCATCgtg < 1:237151/143‑1 (MQ=255) agagCGTAAAGCCATTGCCCGCTTTGCTATCGATCATGTCGATCGTCGCGTGCCGGTGCTGATCGGCACCGGCGGCACCAACGCCCGGGAAACCATCGAACTCAGCCAGCACGCGCAGCAGGCGGGCGCGGACCGCGTAgtgg > 1:977377/1‑143 (MQ=255) tAAAGCCATTGCCCGCTTTGCTATCGATCATGTCGATCGTCGCGTGCCGGTGCTGATCGGCACCGGCGGCACCAACGCCCGGGAAACCATCGAACTCAGCCAGCACGCGCAGCAGGCGGGCGCGGCCGGCCTCCTGGTGCTcc > 1:271960/1‑142 (MQ=255) aaaGCCATTGCCCGCTTTGCTATCGATCATGTCGATCGTCGCGTGCCGGTGCTGATCGGCACCGGCGGCACCAACGCCCGGGAAACCATCGAAATCAGCACTGGCTTGGGGCAGGCGGGCGCGGACGGCCTCGCGGTGAGCaa > 2:902364/1‑143 (MQ=255) cATTGCCCGCTTTGCTATCGATCATGTCGATCGTCGCGTGCCGGTGCTGATCGGCCCCGGCGGCACCAACGCCCGGGAAACCATCGAACTCAGCCAGCACGCGCCGCAGGCGGGCGCGGACGGCAACGTGGCGATCAACCCct > 2:796344/1‑143 (MQ=255) gCTATCGATCATGTCGATCGTCGCGTGCGGGTGCTGATCGGCACCGGCGGCACCAACGCCCGGGAAACCATCGAACTCAGCCAGCACGCGCAGCAGGCGGGCGCGGACGGCATCGTGGTGATCAACCCCTACTACTGGAAAgt < 1:518859/143‑1 (MQ=255) tATCGATCATGTCGATCGTCGCGTGCCGGTGCTGATCGGCAACGGTGGACCTGACGCCCGGGAAAGTATCGCCGGCACCCAGCACGCGCAGCAGGCGGGCCCGGACGGCATCGTGGTGATCAACCCCCACTACTGGAAAgcgt > 1:595241/1‑143 (MQ=255) aTCGATCATGTCGATAGTCGCGTGCCGGTGCTGATCGGCACCGGCGGCACCAACGCCCGGGAAACCATCGAACTCAGCCAGCACGCGCAGCAGGCGGGCGCGGACGGCATCGTGGTGATCAACCCCTACTCCTGGAAAGTGTc > 2:1078024/1‑143 (MQ=255) gTCGATCGTCGCGTGCCGGTGCTGATCGGCACCGGCGGCACCAACGCCCGGGAAACCATCGAACTCAGCCAGCACGCGCAGCAGGCGGGCGCGGACGGCATCGTGGTGATCAACCCCTACTACTGGAAAGTGTCGGAAGCgga < 2:1009441/143‑3 (MQ=255) gTCGATCGTCGCGTGCCGGTGCTGATCGGCACCGGCGGCACCAACGCCCGGGAAAACTGCGGACGCAGCCAGCACGCGCAGCAGGCGGGCGCGGACGGCATCGTGGTGATCACCCCCCACTGCTGGAAAGTGTCGGAAGCCaa > 1:873488/1‑143 (MQ=255) aTCGGCGCGTGCCGGTGCTGGTCGGCACCGGCGGCACCAACGCCCGGGAAACCATCGAACTCAGCCAGCACGCGCAGCAGGCGGGCGCGGACGGCATCGTGGTGATCAACCCCTACTACTGGAAAGTGTCGGAAGCGAACCTg < 2:527124/143‑1 (MQ=255) cgtcgCGTGCCGGTGCTGATCGGCACCGGCGGCACCAACGCCCGGGAAACCATCGAACTCAGCCAGCACGCGCAGCAGGCGGGCGCGGACGGCATCGTGGTGATCAACCCCTACTACGGGAAAGTGTCGGAAGCGAACCTGAt < 1:579032/143‑1 (MQ=255) gtcgCGTGCCGGTGCTGATCGGCACCGGCGGCACCAACGCCCGGGAAACCATCGAACTCAGCCAGCACGCGCAGCAGGCGGGCGCGGACGGCATCGTGGTGATCAACCCCTACTACTGGAAAGTGTCGGAAGCGAACCTGATc < 1:431683/143‑1 (MQ=255) tcgCGTGCCGGTGCTGATCGGCACCGGCGGCACCAACGCCCGGGAAACCATCGAACTCAGCCAGCACGCGCGGCAGGCGGGCGCGGACGGCATCGTGGTGATCACCCCCTACTCCTGGCAAGTGTCGGCAGCGAACCTCATcc > 1:789283/1‑143 (MQ=255) gCCGGTGCCGATCAGCACCGGCGGCACCAACGCCCGGGAAACCATCGAACTCAGCCAGCACGCGCAGCAGGCGGGCGCGGACGGCATCGTGGTGATCAACCCCTACTACTGGAAAGTGTCGGAAGCGCACCTGGTCCGCTAtt > 1:459933/1‑143 (MQ=255) gCCGGTGCCGATCAGCACCGGCGGCACCAACGCCCGGGAAACCATCGAACTCAGCCAGCACGCGCAGCAGGCGGGCGCGGACGGCATCGTGGTGATCAACCCCTACTACTGGAAAGTGTCGGAAGCGAACCTGATCCGCTAtt > 1:619898/1‑143 (MQ=255) ccGGTGCTGATCGGCACCGGCGGCACCAACGCCCGGGAAACCATCGAACTCAGCCAGCACGCGCAGCAGGCGGGCGCGGACGGCATCGTGGTGATCAACCCCTACTACTGGAAAGTGTCGGAAGCGAACCTGATCCGCTAttt < 2:503307/143‑1 (MQ=255) ccGGTGCTGATCGGCACCGGCGGCACCAACGCCCGGGAAACCATCGAACTCAGCCAGCACGCGCAGCAGGCGGGCGCGGACGGCATCGTGGTGATCAACCCCTACTACTGGAAAGTGTCGGAAGCGAACCTGATCCGCTAttt < 1:1187094/143‑1 (MQ=255) gcgCTCATCGGCACCGGCCGCCCCAACGCCCGGCAAACCATCGAACTCAGCCAGCACGCGCAGCAGGCGGGCGCGGACGGCATCGTGGTGATCAACCCCTACTACTGGAAAGTGTCGGAAGCGAACCTGATCCGCTATTTCGa < 1:1017355/141‑1 (MQ=255) tGCTGATCGGCACCGGCGGCACCAACGCCCGGGAAACCATCGAACTCAGCCAGCACGCGCAGCAGGCGGGCGCGGACGGCATCGTGGTGATCAACCCCTACTACTGGAAAGTGTCGGAAGCGAACCTGATCCGCTATTTCGAg > 1:1242492/1‑143 (MQ=255) tGATCGGCACCGGCGGCACCAACGCCCGGGAAACCATCGAACTCAGCCAGCACGCGCAGCAGGCGGGCGCGGACGGCATCGTGGTGATCAACCCCTACTACTGGACAGTGTCGGGAGCGGACCTGATCCGCTATTTGGAGCAg > 2:502488/1‑143 (MQ=255) gtgggCACCGGCGGCCCGCGCGCCCGGGCGACCATCGAACTCAGCCAGCACGCGCAGCAGGCGGGCGCGGACGGCATCGTGGTGATCAACCCCTACTACCGGAAAGTGTCGGAAGCGAACCTGATCCGCTATTTCGAGCAGGt < 2:857958/140‑1 (MQ=255) aTCGGCACCGGCGGCACCAACGCCCGGGAAACCATCGAACTCAGCCAGCACGCGCAGCAGGCGGGCGCGGACGGCATCGTGGTGATCAACCCCTACTACTGGAAAGTGCCGGAAGCGACCCTGATCCGCCATATCGAGCAGGt > 2:808581/1‑143 (MQ=255) | CGACGATCTGATCAAAGCAGGCGTTGACGGCCTGTTCTTCCTGGGCAGCGGTGGCGAGTTCTCCCAGCTCGGCGCCGAAGAGCGTAAAGCCATTGCCCGCTTTGCTATCGATCATGTCGATCGTCGCGTGCCGGTGCTGATCGGCACCGGCGGCACCAACGCCCGGGAAACCATCGAACTCAGCCAGCACGCGCAGCAGGCGGGCGCGGACGGCATCGTGGTGATCAACCCCTACTACTGGAAAGTGTCGGAAGCGAACCTGATCCGCTATTTCGAGCAGGT > NC_000913/282376‑282657 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |