BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	4,335,655	T→A	5.9%	intergenic (‑65/+39)	eptA ← / ← adiC	phosphoethanolamine transferase EptA/arginine:agmatine antiporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,335,655	0	T	A	5.9%	396.5 / 14.7	139	intergenic (‑65/+39)	eptA/adiC	phosphoethanolamine transferase EptA/arginine:agmatine antiporter
Reads supporting (aligned to +/- strand): ref base T (57/71); new base A (3/5); total (61/78)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.73e-03

Only 100 of 146 total aligned reads displayed.

GATAGAGATATAAAAAGCGGCCAACAATAGCCAGGCGAGTAAATTCAAAGAGGGTCTTTTTAGTAGGCGCTTCAACATCACGGTGTTTCCATCGAACAAAGTGCGCATATGCTCGCAAAGCAAA‑ATT‑AAGCCAACCTTAAGTTCTTAAGGTTGGCTTTTATGTTTGCTGGATTAAGGGGAATTAATCTTTGCTTATTGGT‑GCATCTAAGGGATACGGGTTTTTATGTAGCCGGTTGTAATTCAGCGCATACATGGCGGTGATGACCATCAGGGTGACAAATGACC  >  NC_000913/4335513‑4335797

gATAGAGATATAAAAAGCGGCCAACAATAGCCAGGCGAGTAAATTCAAAGAGGGTCTTTTTAGTAGGCGCTTCAACATCACGGTGTTTCCATCGAACAAAGTGCGCATATGCTCGCAACGCAAA‑ATT‑AAGCCCAGCTTAAGtt                                                                                                                                                 >  1:1109189/1‑143 (MQ=255)

  tAGAGATATAAAAAGCGGCCAACAATAGCCAGGCGAGTAAATTCAAAGAGGGTCTTTTTAGTAGGCGCTTCAACATCACGGTGTTTCCATCGAACAAAGTGCGCATATGCTCGCAAAGGAAA‑ATT‑AAGCCAACATTAAGTTCt                                                                                                                                               >  1:199253/1‑143 (MQ=255)

  tAGAGATATAAAAAGCGGCCAACAATAGCCAGGCGAGTAAATTCAAAGAGGGTCTTTTTAGTAGGCGCTTCAACATCACGGTGTTTCCATCGAACAAAGTGCGCATATGCTCGCAAAGCACA‑ATT‑AAGCCAACCTCACGTTCt                                                                                                                                               >  2:306557/1‑143 (MQ=255)

      gaTATAAAAAGCGGCCAACAATAGCCAGGCGAGTAAATTCAAAGAGGGTCTTTTTAGTAGGCGCTTCAACATCACGGTGTTTCCATCGAACAAAGTGCGCATATGCTCGCAAAGCAAA‑ATT‑AAGCCAACCTTAAGTTCTTAAg                                                                                                                                           <  1:1230434/143‑1 (MQ=255)

      gaTATAAAAAGCGGCCAACAACAGCCAGGCGAGTATATTCAAAGAGGGTCTTTTTAGTAGGCGCTTCAACATCACGGTGTTTCCATCGAACAAAGTGCGCATATGCTCGCAAAGCAAA‑ATT‑AAGCCAACCTTAAGTTCTTAAg                                                                                                                                           <  1:40657/143‑1 (MQ=255)

         ataAAGCGCGGCCAACAGTAGCCAGGCGAGTAACGTCAAAGTGGGTCTGTGTAGTAGGCGCTTCAACAGCACGGGGTTTCCATCGAACAAAGTGCGCATATGCTCGCAAAGCAAA‑ATT‑AAGCCAACCTTAAGTTCTTAAGGtt                                                                                                                                        <  2:212897/143‑1 (MQ=255)

         ataAAAAGCGGCCAACAATAGCCAGGCGAGTAAATTCAAAGAGGGTCTTTTTAGTAGGCGCTTCAACATCACGGTGTTTCCATCGAACAAAGTGCGCATATGCTCGCAAAGCAAA‑ATT‑AAGCCAACCTTAAGAACTTAAGGtt                                                                                                                                        <  1:1158949/143‑1 (MQ=255)

         ataAAAAGCGGCCAACAATAGCCAGGCGAGTAAATTCAAAGAGGGTCTTTTTAGTAGGCGCTTCAACATCACGGTGTTTCCATCGAACAAAGTGCGCATATGCTCGCAAAGCAAA‑ATT‑AAGCCAACCTTAAGAACTTAAGGtt                                                                                                                                        <  1:1184601/143‑1 (MQ=255)

         ataAAAAGCGGCCAACAAGAGCCAGGCGAGTAAATTCAAAGAGGGTCTTTTTAGTAGGCGCTTCAACATCACGGTGTTTCCATCGAACAAAGTGCGCATATGCTCGCAAAGCAAA‑ATT‑AAGCCAACCTTAAGTTCGTAAGGtt                                                                                                                                        <  2:1142301/143‑1 (MQ=255)

          taAAAAGCGGCCAACAATAGCCAGGCGAGTAAATTCAAAGAGGGTCTTTTTAGTAGGCGCTTCAACATCACGGTGTTTCCATCGAACAAAGTGCGCATATGCTCGCAAAGCAAA‑ATT‑AAGCCAACCTTAAGTTCTTAAGGTTg                                                                                                                                       >  2:25518/1‑143 (MQ=255)

          taAAAAGCGGCCAACAATAGCCAGGCGAGTAAATTCAAAGAGGGTCTTTTTAGTAGGCGCTTCAACATCACGGTGTTTCCATCGAACAAAGTGCGCATATGCTCGCAAAGCAAA‑ATT‑AAGCCAACCTTAAGTTCTTAAGGTTg                                                                                                                                       >  1:936795/1‑143 (MQ=255)

           aaaaaGCGGCCAACAATAGCCAGGCGAGTAAATTCAAAGAGGGTCTTTTTAGTAGGCGCTTCAACATCACGGTGTTTCCATCGAACAAAGTGCGCATATGCTCGCAAAGCAAA‑ATT‑AAGCCAACCTTAAGTTCTTACGGTTgg                                                                                                                                      >  1:917174/1‑143 (MQ=255)

           aaaaaGCGGCCAACAATAGCCAGGCGAGTAAATTCAAAGAGGGTCTTTTTAGTAGGCGCTTCAACATCACGGTGTTTCCATCGAACAAAGTGCGCATATGCTCGCAAAGCAAA‑ATT‑AAGCCAACCTTAAGTTCTTAAGGTTgg                                                                                                                                      <  1:724551/143‑1 (MQ=255)

            aaaaGCGGCCAACAATAGCCAGGCGAGTAAATTCAAAGAGGGTCTTTTTAGTAGGCGCTTCAACATCACGGTGTTTCCATCGAACAAAGTGCGCATATGCTCGCAAAGCAAACATAAAAGCCAACCTTAAGAACTTAAGGTTg                                                                                                                                       <  2:656048/143‑1 (MQ=38)

                    ccAACAATAGCCAGGCGAGTAAATTCAAAGAGGGTCTTTTTAGTAGGCGCTTCAACATCACGGTGTTTCCATCGAACAAAGTGCGCATATGCTCGCAAAGCAAA‑ATT‑AAGCCAACCTTAAGTTCTTAAGGTTGGCTGTTATGt                                                                                                                             >  1:397888/1‑143 (MQ=255)

                       acaaTAGCCAGGCGAGTAAATTCAAAGAGGGTCTTTTTAGTAGGCGCTTCAACATCACGGTGTTTCCATCGAACAAAGTGCGCATATGCTCGCAAAGCAAA‑ATT‑AAGCCAACCTTAAGTTCTTAAGGTTGGCTTTTATGTTTg                                                                                                                          <  1:1193952/143‑1 (MQ=255)

                         aaTAGCCAGGCGAGTAAATTCAAAGAGGGTCTTTTTAGTAGGCGCTTCAACATCACGGTGTTTCCATCGAACAAAGTGCGCATATGCTCGCAAAGCAAA‑ATT‑AAGCCAACCTTAAGTTCTTAAGGTTGGCTTTTATGTTTGCt                                                                                                                        <  2:937377/143‑1 (MQ=255)

                         aaTAGCCAGGCGAGTAAATTCAAAGAGGGTCTTTTTAGTAGGCGCTTCAACATCACGGTGTTTCCATCGAACAAAGTGCGCATATGCTCGCAAAGCAAA‑ATT‑AAGCCAACCTTAAGTTCTTAAGGTTGGCTTTTATGTTTGCt                                                                                                                        <  1:983033/143‑1 (MQ=255)

                         aaTAGCCAGGCGAGTAAATTCAAAGAGGGTCTTTTTAGTAGGCGCTTCAACATCACGGTGTTTCCATCGAACAAAGTGCGCATATGCTCGCAAAGCAAA‑ATT‑AAGCCAACCTTAAGTCCTTAAGGTTGGCTTTTATGTTTGCt                                                                                                                        >  2:1021808/1‑143 (MQ=255)

                         aaTAGCCAGGCGAGTAAATTCAAAGAGGGTCTTTTTAGTAGGCGCTTCAACATCACGGTGTTTCCATCGAACAAAGTGCGCATATGCGCGCAAAGCAAA‑ATT‑AAGCCAACCTTAAGTTCTTAAGGTTGGCTTTTATGTTTGCt                                                                                                                        <  1:1229665/143‑1 (MQ=255)

                            aGCCAGGCGAGTAAATTCAAAGAGGGTCTTTGTAGTAGGCGCTTCAACATCACGGTGTTTCCATCGAACAAAGTGCGCATATGCTCGCAAAGCAAA‑ATT‑AAGCCAACCTTAAGTTCTTAAGGTTGGCTTCTATGTTTGCTGGa                                                                                                                     <  1:552800/143‑1 (MQ=255)

                            aGCCAGGCGAGTAAATTCAAAGAGGGTCCTTTTAGTAGGCGCTCCAACATCACGGTTTTTCCATCGAACAAAGTGCGCATATGCTCGCAAAGCAAA‑ATT‑AAGCCAACCTTAAGTTCTTAAGGTTGGCTTTTATGTTTGCTGGa                                                                                                                     <  1:1069332/143‑1 (MQ=255)

                                 ggCGAGTAAATTCCAAGAGGGTCTTTTTAGTAGGCGCTTCAACATCACGGTGTTTCCATCGAACAAAGCGCGCAAATCTGCGCATAGCAAA‑ATT‑AAGCCAACCTTAAGTTCTTAAGGTTGGCTTTTATGTTTGCTGGATTAAg                                                                                                                <  1:180282/143‑1 (MQ=255)

                                    gattAAATTCTAAGAGCGTCTTTTTAGTAGGCGCTTCAACATCACGGTGTTTCCATCGAACAAAGTGCGCATATGCTCGCAAAGCAAA‑ATT‑AAGCCAACCTTAAGTTCTTAAGGTTGGCTTTTATGTTTGCTGGATTAAgggg                                                                                                             <  1:749290/140‑1 (MQ=255)

                                      gTAAATTCAAAGAGGGTCTTTCTAGTAGGCGCTTCAACATCACGGTGTTTCCATCGAACAAAGTGCGCATATGCTCGCAAAGCAAA‑ATT‑AAGCCAACCTTAAGTTCTTAAGGTTGGCTTTTATGTTTGCTGGATTAAGGGGaa                                                                                                           <  1:645510/143‑1 (MQ=255)

                                       tAAATTCAAAGAGGGTCTTTTTAGTAGGCGCTTCAACATCACGGTGTTTTCATCGAACAAAGTGCGCATATGCTCGCAAAGCAAA‑ATT‑AAGCCAACCTTAAGTTCTTAAGGTTGGCTCTTATGTTTGCTGGATTAAGGGGAAt                                                                                                          >  1:152431/1‑143 (MQ=255)

                                       tAAATTCAAAGAGGGTCTTTTTAGTAGGCGCTTCAACATCACGGTGTTTCCATCGAACAAAGTGCGCATATGCTCGCAAAGCAAA‑ATT‑AAGCCAACCTTAAGTTCTTAAGGTTGGCTTTTATGTTTGCTGGATTAAGGGGAAt                                                                                                          <  1:1089500/143‑1 (MQ=255)

                                         aaTTCAAAGAGGGTCTTTTTAGTAGGCGCTTCAACATCACGGTGTTTCCATCGAACAAAGTGCGCATATGCTCGCAAAGCAAA‑ATT‑AAGCCAACCTTAAGTTCTTAAGGTTGGCTTTTATGTTTGCTGGATTAAGGGGAATTa                                                                                                        <  1:1257817/143‑1 (MQ=255)

                                           ttCAAAGAGGGTCTTTTTAGTAGGCGCTTCAACATCACGGTGTTTCCATCGAACAAAGTGCGCATATGCTCGCAAAGCAAA‑ATT‑AAGCCAACCTTAAGTTCTTAAGGTTGGCTTTTATGTTTGCTGGATTAAGGGGAATTAAt                                                                                                      <  1:712205/143‑1 (MQ=255)

                                                 gagGGTCTTTTTAGTAGGCGCTTCAACATCACGGTGTTTCCATCGAACAAAGTGCGCATATGCTCGCAAAGCAAA‑ATT‑AAGCCAACCTTAAGTTCTTAAGGTTGGCTTTTATGTTTGCTGGATTAAGGGGAATTAATCTTTGc                                                                                                <  2:66375/143‑1 (MQ=255)

                                                    ggTCTTTTTAGTAGGCGCTTCAACATCACGGTGTTTCCATCGAACAAAGTGCGCATATGCTCGCAAAGCAAA‑ATT‑AAGCCAACCTTAAGTTCTTAAGGTTGGCTTTTATGTTTGCTGGATTAAGGGGAATTAATCTTTGCTTa                                                                                             >  1:489965/1‑143 (MQ=255)

                                                      tCTTTTTAGTAGGCGCTTCAACATCACGGTGTTTCCATCGAACAAAGTGCGCATATGCTCGCAAAGCAAA‑ATT‑AAGCCAACCTTAAGTTCTTAAGGTTGGCTTTTATGTTTGCTGGATTAAGGGGAATTAATCTTTGCTTAtt                                                                                           <  1:1127102/143‑1 (MQ=255)

                                                       cTTTTTAGTAGGTCCTTCAACATCACGTCGTTTCCATCGAACAAACTGCGCATATGCTCGCAAATCAAA‑ATT‑AATCCAACCTTAACTTCTTAAGGTTGGCTTTTATGTTTGCTGGATTAAGGGGAATTAATCTTTGCTTATTg                                                                                          <  2:40931/143‑1 (MQ=255)

                                                        tttttAGTAGGCGCTTCAACATCACGGTGTTTCCATCGAACAAAGTGCGCATATGCTCGCAAAGCAAA‑ATT‑AAGCCAACCTTAAGTTCTTAAGGTTGGCTTTTATGTTTGCTGGATTAAGGGGAATTAATCTTTGCTTATTgg                                                                                         >  2:784514/1‑143 (MQ=255)

                                                         ttttAGTAGGCGCTTCAACATCACGGTGTTTCCATCGAACCAAGTGCGCATATGCTCGCAGAGCAAA‑ATT‑AAACCAACGTGGAGTTCTTAAGGTTGGCTTTTATGTTTTCTGGATTAAGGGGACTTCATCTTTGATTATTGGt                                                                                        >  2:1110875/1‑143 (MQ=255)

                                                         ttttAGTAGGCGCTTCAACATCACGGTGTTTCCATCGAACAAAGTGCGCATATGCTCGCAAAGCAAA‑ATT‑AAGCCAACCTTAAGTTCTTAAGGTTGGCTTTTATGTTTGCTGGATTAAGGGGAATTAATCTTTGCTTATTGGt                                                                                        <  2:890500/143‑1 (MQ=255)

                                                          tttAGTAGGCGCTTCAACATCACGGTGTTTCCATCGAACAAAGTGCGCATATGCTCGCAAAGCAAA‑ATT‑AAGCCAACCTTAAGTTCTTAAGGTTGGCTTTTATGTTTGCTGGATTAAGGGGAATTAATCTTTGCTTATTGGT‑g                                                                                      <  1:500609/143‑1 (MQ=255)

                                                          tttAGTAGGCGCTTCAACATCACGGTGTTTCCATCGAACAAAGTGCGCATATGCTCGCAAAGCAAA‑ATT‑AAGCCAACCTTAAGTTCTTAAGGTTGGCTTTTATGTTTGCTGGATTAAGGGGAATTAATCTTTGCTTATTGGT‑g                                                                                      >  1:993881/1‑143 (MQ=255)

                                                          tttAGTAGGCGCTTCAACATCACGGTGTTTCCATCGAACAAAGTGCGCATATGATCGCAAAGCAAA‑ATT‑GCGCCAACCTTAAGTTCTTAAGGTTGGCGTTTATGTTTGCTGGTGGACGGGGACTTAATCTTTGCTTATTGGtc                                                                                       >  1:614214/1‑142 (MQ=255)

                                                          tttAGTAGGCGCTTCAAACTCAAGGTGCTTCCACCGAACAAAGTGCACATATGCTCGAACTGAGAA‑TTC‑AAGCCATCATTAAGTTCTTAAGGTTGGCTTTTCAATTTGCTTGATTAAGGGGAATTAATCTTTGCTTATTGGT‑g                                                                                      <  2:325753/143‑1 (MQ=255)

                                                           ttAGTAGGCGCTTCAACGTCACGGTGTTTCCATCGAACAAAGTGCGCATATGCTCGCAAAGCAAA‑ATT‑AAGCCAACCTTAAGTTCTTAAGGTTGGCTTTTATGTTTGCTGGATTAAGGGGAATTAATCTTTGCTTATTGGT‑Gc                                                                                     <  2:115113/143‑1 (MQ=255)

                                                           ttAGTAGGCGCTTCAACATCACGGTGTTTCCATCGAACAAAGTGCGCATATGCTCGCAAAGCAAA‑ATT‑AAGCCAACCTTAAGTTCTTAAGGTTGGCTTTTATGTTTGCTGGATTAAGGGGAATTAATCTTTGCTTATTGGT‑Gc                                                                                     >  2:947816/1‑143 (MQ=255)

                                                               tagGCGCTTCAACATCACGGTGTTTCCATCGAACAAGGTGCGCATATGCTCGCAAAGCAAA‑ATT‑AAGCCAACCTTAAGTTCTTAAGGTTGGCTTTTATGTTTGCTGGATTAAGGGGAATTAATCTTTGCTTATTGGT‑GCATCt                                                                                 >  1:607393/1‑143 (MQ=255)

                                                                agGCGCTTCAACATCACGGTGTTTCCATCGAACAAAGTGCGCATATGCTCGCAAAGCAAA‑ATT‑AAGCCAACCTTAAGTTCTTAAGGTTGGCTTTTATGTTTGCTGGATTAAGGGGAATTAATCTTTGCTTATTGGT‑GCATCTa                                                                                >  2:327570/1‑143 (MQ=255)

                                                                 ggCGCTTCAACATCACGGTGTTTCCATCGAACAAAGTGCGCATATGCTCGCAAAGCAAA‑ATT‑AAGCCAACCTTAAGTTCTTAAGGTTGGCTTTTATGTTTGCTGGATTAAGGGGAATTAATCTTTGCTTATTGGT‑GCATCTaa                                                                               <  1:644061/143‑1 (MQ=255)

                                                                  gcgcTTCAACATCACGGTGTTTCCATCGAACAAAGTGCGCATATGCTCGCAAAGCAAA‑GTT‑GAGCCAACCTTAAGTTCTTAAGGTTGGCTTTTATGTTTGCTGGATTAAGGGGAATTAATCTTTGCTTATTGGT‑GCATCTAAg                                                                              <  2:1110131/143‑1 (MQ=255)

                                                                  gcgcTTCAACATCACGGTGTTTCCATCGAACAAAGTGCGCATATGCTCGCAAAGCAAA‑ATT‑AAGCCAACCTTAAGTTCTTAAGGTTGGCTTTTATGTTTGCTGGATTAAGGGGAATTAATCTTTGCTTATTGGT‑GCATCTAAg                                                                              <  1:222829/143‑1 (MQ=255)

                                                                     cTTCAACATCACGGTGTTTCCATCGAACAAAGTGCGCATATGCTCGCAAAGCAAA‑ATT‑AAGCCAACCTTAAGTTCTTACGGTTGGCTTTTATGTTTGCTGGATTAAGGGGAATTAATCTTTGCTTATTGGT‑GCATCTAAGGGa                                                                           <  1:973362/143‑1 (MQ=255)

                                                                     cTTCAACATCACGGTGTTTCCATCGAACAAAGTGCGCATATGCTCGCAAAGCAAA‑ATT‑AAGCCAACCCACAGTTCTTAAGGTTGGCTCTTACGTTTGCTGGCTTCAAGGGAATTAAGCATTGCGTATTGGC‑GCCTCGAAgcaa                                                                           >  2:542385/1‑140 (MQ=255)

                                                                      ttCAACATCACGGTGTTTCCATCGAACAAAGTGCGCATATGCTCGCAAAGCAAA‑ATT‑AAGCCAACCTTACGGTGGTAAGGTAGGCTGTTATGGTTGCTGGAGTACGGGGAATTAATCTTTGCTTATGGGT‑GCAACTAAGGGAt                                                                          >  1:442698/1‑143 (MQ=255)

                                                                       tCAACATCACGGTGTTTCCATCGAACAAAGTGCGCATATGCTCGCAAAGCAAA‑ATT‑AAGCCAACCTTAAGTTCTTAAGGTTGGCTTTTATGTTTGCTGTATTAAGTGGAATTAATCTTTGCTTATTGGT‑GCATCTAAGGGATa                                                                         <  2:1044307/143‑1 (MQ=255)

                                                                          aCATCACGGTGGTTCAATCGAACAAAGTGCGCATATGCTCGCAAAGCAAA‑ATT‑AAGCCAACCTTAAGTTCTTAAGGTTGGCTTTTATGTTTGCTGGATTAAGGGGAATTAATCTTTGCTTATTGGT‑GCATCTAAGGGATACgg                                                                      <  2:481300/143‑1 (MQ=255)

                                                                                 ggTGTTTCCATCGAACAAAGTGCGCATATGCTCGCAAAGCAAA‑ATT‑AAGCCAACCTTAAGTTCTTAAGGTTGGCTTTTATGTTTGCTGGATTAAGGGGAATTAATCTTTGCTTATTGGT‑GCATCTAAGGGATACGGGTTTTTa                                                               <  1:241219/143‑1 (MQ=255)

                                                                                  gtgtTTCCATCGAACAAAGTGCGCATATGCTCGCAAAGCAAA‑ATT‑AAGCCAACCTTAAGTTCTTAAGGCTGGCTTTTATGTTTGCTGGGTTAAGGGGAATTAATCTTTGCTTATTGGT‑GCATCTAAGGGATACGGGTTTTTAt                                                              >  2:1255773/1‑143 (MQ=255)

                                                                                  gtgtTTCCATCGAACAAAGTCCGCATATGCTCGCAAAGCAAA‑ATT‑AAGCCAACCTTAAGTTCTTAAGGTTGGCTTTTATGTTTGCTGGATTAAGGGGAATTAATCTTTGCTTATTGGT‑GCATCTAAGGGATACGGGTTTTTAt                                                              <  1:845795/143‑1 (MQ=255)

                                                                                     ctgccATCAAACAAAGTGCGCATATGCGCGCAAAGCAAA‑ATT‑CAGCCAACCTTAAGTTCTTAAGGTTGGCTTTTATGTTTGCTGGATTAAGGGGAATTAATCTTTGCTTATTGGT‑GCATCTAAGGGATACGGGTTTTTATGTa                                                           <  1:1165526/140‑1 (MQ=255)

                                                                                      ttCCATCGAACAAAGTGCGCATATGCTCGCAAAGCAAA‑ATT‑AAGCCAACCTTAAGTTCTTAAGGTTGGCTTTTATGTTTGCTGGATTAAGGGGAATTAATCTTTGCTTATTGGT‑GCATCTAAGGGATACGGGTTTTTATGTAg                                                          >  2:182606/1‑143 (MQ=255)

                                                                                         cATCGAACAAAGTGCGCATATGCTCGCAAAGCAAA‑ATT‑AAGCCAACCTTAAGTTCTTAAGGTTGGCTTTTATGTTTGCTGGATTAAGGGGAATTAATCTTTGCTTATTGGT‑GCATCTAAGGGATACGGGTTTTTATGTAGCCg                                                       >  1:329860/1‑143 (MQ=255)

                                                                                          aTCGAACAAAGTGCGCATATGCTCGCAAAGCAAA‑ATT‑AAGCCAACCTTAAGTTCTTAAGGTTGGCTTTTATGTTTGCTGGATTAAGGGGAATTAATCTTTGCTTATTGGT‑GCATCTAAGGGATACGGGTTTTTATGTAGCCgg                                                      <  1:8703/143‑1 (MQ=255)

                                                                                          aTCGAACAAAGTGCGCATATGCTCGCAAAGCAAA‑ATT‑AAGCCAACCTTAAGTTCTTAAGGTTGGCTTTTATGTTTGCTGGATTAAGGGGAATTAATCTTTGCTTATTGGT‑GCATCTAAGGGATACGGGTTTTTATGTAGCCgg                                                      >  2:60212/1‑143 (MQ=255)

                                                                                              aaCAAAGTGCGCATATGCTCGCAAAGCAAA‑ATT‑AAGCCAACCTTAAGTTCTTAAGGTTGGCTTTTATGTTTGCTGGATTAAGGGGAATTAATCTTTGCTTATTGGT‑GCATGTAAGGGATACGGGTTTTTATGTAGCAGGTTGt                                                  >  1:231977/1‑143 (MQ=255)

                                                                                              aaCAAAGTGCGCATATGCTCGCAAAGCAAA‑ATT‑AAGCCAACCTTAAGTTCTTAAGGTTGGCTTTTATGTTTGCTGGATTAAGGGGAATTAATCTTTGCTTATTGGT‑GCATCTAAGGGAGACGGGATTTTATGTAGCCGGTTGt                                                  >  1:1225650/1‑143 (MQ=255)

                                                                                                    gTGCGTATTTGCTTGTGATGCGAA‑ATT‑AATCCAACCTTAAGTTCTTAAGGTTGGCTTTTATGTTTGCTGGATTAAGGGGAATTAATCTTTGCTTATTGGT‑GCATCTAAGGGATACGGGTTTTTATGTAGCCGGTTGTAATTCa                                            <  2:1023692/143‑1 (MQ=255)

                                                                                                    gTGCGCATATGCTCGCAAAGCAAA‑ATT‑AAGCCAACCTTAAGTTGTTAAGGTTGGCTTTTATGTTTGCTGGATTAAGGGGAATTAATCTTTGCTTATTGGT‑GCATCTAAGGGATACGGGTTTTTATGTAGCCGGTTGTAATTCa                                            <  1:1273170/143‑1 (MQ=255)

                                                                                                    gTGCGCATATGCTCACAAAGCAAA‑ATT‑AAGCCAACCTTAAGTTCTTAAGGTTGGCTTTTATGTTTGCTGGATTAAGGGGAATTAATCTTTGCTTATTGGT‑GCATCTAAGGGAGACGGGCGTTTATGTAGCCGGTTGTAATACa                                            >  2:1192756/1‑143 (MQ=255)

                                                                                                     tGCGCATATGTTCTCAAAGCAAA‑ATT‑AAGCCAACCTTAAGTTCTTAAGGTTGGCTTTTATGTTTGCTGGATTAAGGGGAATTAATCTTTGCTTATTGGT‑GCATCTAAGGGATACGGGTTTTTATGTAGCCGGTTGTAATTCAg                                           <  1:1143247/143‑1 (MQ=255)

                                                                                                       cgtatatGGTCGCAAAGCAGA‑GTT‑AAGCTGACCTTAAGTTCTTAAGGTTGGCTTGTATGTTTGCTGGATTAAGGGGAATTAATCTTTGCTTATTGGT‑GCATCTAAGGGATACGGGTTTTTATGTAGCCGGTTGTAATTCAgcg                                         <  2:863520/140‑1 (MQ=255)

                                                                                                       cgcATATGCTCGCAAAGCAAA‑ATT‑AAGCCAACCTTAAGTTCTTAAGGTTGGCTTTTATGTTTGCTGGATTAAGGGGAATTAATCTTTGCTTATTGGT‑GCATCTAAGGGATACGGGTTTTTATGTAGCCGGTTGTAATTCAgcg                                         <  2:225293/143‑1 (MQ=255)

                                                                                                       cgcATATGCTCGCAAAGCAAA‑ATT‑AAGCCAACCTTAAGTTCTCAAGGTTGGCTTTTATGTTTGTTGTCTTCCGACTAACAAAT‑TTAAGGTATCGTTATGATCTAAGGGATACGGGTTTTTATGTAGCCGGTTGTAATTCAgcg                                         <  1:595384/143‑1 (MQ=255)

                                                                                                           tatGCTCGCAAAGCAAA‑ATT‑AAGCCAACCTTAAGTTCTTAAGGTTGGCTTTTATGTTTGCTGGATTAAGGGGAATTAATCTTTGCTTATTGGT‑GCATCTAAGGGATACGGGTTTTTATGTAGCCGGTTGTAATTCAGCGCATa                                     >  1:1114125/1‑143 (MQ=255)

                                                                                                                tCGCAAAGCAAA‑ATT‑AAGCCAACCTTAAGTTCTTAAGGGTGGCGTTTATGTTTGCTGGATTAAGGGGAATTAATCTTTGCTTATTGGT‑GCATCTAAGGGATACGGGTTTTTATGTAGCCGGTTGTAATTCGGCGCATACATgg                                >  1:256784/1‑143 (MQ=255)

                                                                                                                 cGCAAAGGAAA‑ATT‑ATGCCAACCGTATGTGCTTAAGGTTGGCTTTTATGTTTGCTGGATTAAGGGGAATTAATCTTTGCTTATTGGT‑GCATCTAATGGATACGGGTTTTTATGTAGCCGGTTGTAATTCAGCGCATACATGGc                               <  2:571992/143‑1 (MQ=255)

                                                                                                                  gcaaagcaaa‑ATT‑AAGCCAACCTTAAGTTCTTAAGGTTGGCTTTTATGTTTGCTGGATTAAGGGGAATTAATCATTGCTGATTGGT‑GCATCTAAGGGATACGGGTTTTTATGTAGCCGGGTGTCATTCAGCGGATACATGGCg                              >  2:82015/1‑143 (MQ=255)

                                                                                                                   caaagcaaa‑ATT‑AAGCCAACCTTAAGTTCTTAAGTTTGGCTTTTATGTTTTCTTGATTAAGGGGAATTAATCTTTGCTTATTGGT‑GCATCTAAGGGATACGGGTTTTTATGTAGCCGGTTGTAATTCAGCGCATACATGGCgg                             <  2:1036404/143‑1 (MQ=255)

                                                                                                                    aaagcaaa‑ATT‑AAGCCAACCTTAAGTTCTTAAGGTTGGCTTTTATGTTTGCTGGATTAAGGGGAATTAATCTTTGCTTATTGGT‑GCATCTAAGGGATACGGGTTTTTATGTAGCCGGTTGTAATTCAGCGCATACATGGCGGt                            >  1:885814/1‑143 (MQ=255)

                                                                                                                    aaagcaaa‑ATT‑AAGCCAACCTTAAGTTCTTAAGGTTGGCTTTTATGTTTGCTGGATTAAGGGGAATTAATCTTTGCTTATTGGT‑GCAGCTAAGGGATACGGGTTTTTATGTAGCCGGTTGTAATTCAGCGCATACATGGCGGt                            >  2:38125/1‑143 (MQ=255)

                                                                                                                     aagcaaa‑ATT‑AAGCCCACCTTAAGTGCTTAAGGTGGGCTTCTATGTTTGCTGGATTAAGGGGAATTCATCTTTGCTTATTGGT‑GCATCTAAGGGATACGGGTTTTTATGTAGTCGGTTGTAATTCAGCGCATACATGGCGGtg                           <  2:285624/143‑1 (MQ=255)

                                                                                                                      agcaaa‑ATT‑AAGCCAACCTTAAGTTCTTAAGGTTGGCTTTTATGTTTGCTGGATTAAGGGGAATTAATCTTTGCTTATTGGT‑GCATCTAAGGGATACGGGTTTTTATGTAGCCGGTTGTAATTCAGCGCATACATGGCGGtga                          <  1:25518/143‑1 (MQ=255)

                                                                                                                      agcaaa‑ATT‑AAGCCAACCTTAAGTTCTTAAGGTTGGCTTTTATGTTTGCTGGATTAAGGGGAATTAATCTTTGCTTATTGGT‑GCATCTAAGGGATACGGGTTTTTATGTAGCCGGTTGTAATTCAGCGCATACATGGCGGtga                          <  1:882360/143‑1 (MQ=255)

                                                                                                                        caaa‑ATT‑AAGCCAACCTTAAGTTCTTAAGGTCGGCTTTTATGTTTGCTGGATTAAGGGGAATTAATCTTTGCTTATTGGT‑GCATCTAAGGGATACGGGTTTTTATGTAGCCGGTTGTACTTCAGCCCATACCGGGCGGtgctg                        >  2:1212001/1‑143 (MQ=255)

                                                                                                                         aaa‑aTT‑AAGCCAACCTTAAGTTCTTAAGGTTGGCTTTTATGTTTGCTGGATTAAGGGGAATTAATCTTTGCTTATTGGT‑GCATCTAAGGGATACGGGTTTTTATGTAGCCGGTTGTAATTCAGCGCATACATGGCGGtgatga                       <  1:1266935/143‑1 (MQ=255)

                                                                                                                         aaa‑aTT‑AAGCCAACCTTAAGTTCTTAAGGTTGGCTTTTATGTTTGCTGGATTAAGGGGAATTAATCTTTGCTTATTGGT‑GCATCTAAGGGATACGGGTTTTTATGTAGCCGGTTGTAATTCAGCGCATACATGGCGGtgatga                       >  1:168805/1‑143 (MQ=255)

                                                                                                                         aaa‑aTT‑AAGCCAACCTTAAGTTCTTAAGGTTGGCTTTTATGTTTGCTGGATTAAGGGGAATTAATCTTTGCTTATTGGT‑GCATCTAAGGGATACGGGTTTTTATGTAGCCGGTTGTAATTCAGCGCATACATGGCGGtgatga                       <  1:166134/143‑1 (MQ=255)

                                                                                                                           a‑aTT‑AAGCCAACCTTAAGTTCTTAAGGTTGGCTTTTATGTTTGCTGGATTAAGGGGAATTAATCTTTGCTTATTGGT‑GCATCTAAGGGATACGGGTTTTTATGTAGCCGGTTGTACTTCGGCGCATACATGGCGGTGTTGAcc                     >  2:1275951/1‑143 (MQ=255)

                                                                                                                            aagt‑AAGCCAACCTTAAGTTCTGAAGGTTGGCTGTTAGGTTTGCGGGATTAAGGGGAAGTAATCTTTGCTTATTGGT‑GCATCTAAGGGATACGGGTTTTTATGTAGCCGGTTGTAATTCAGCGCATACATGGCGGTGATGAcc                     <  1:279294/140‑1 (MQ=255)

                                                                                                                             aTT‑AAGCCAACCTTAAGTTCTTAAGGTTGGCTTTTATGTTTGCTGGATTAAGGGGAATTAATCTTTGCTTATTGGT‑GCATCTAAGGGATACGGGTTTTTATGTAGCCGGTTGTAATTCAGCGCATACATGGCAGTGATGGCCa                    >  1:816804/1‑143 (MQ=255)

                                                                                                                             aTT‑AAGCCAACCTTAAGTTCTTAAGGTTGGCTTTTATGTTTGCTGGATGAAGGGGAATTAGTCTTTGCTTATTGGT‑GCATCTAAGGGATACGGGTTTTTATGTAGCCGGTTGTAATTCAGCGCATACATGGCGGTGGTGCCCa                    >  1:968958/1‑143 (MQ=255)

                                                                                                                                    ccAACCTTTAGTTCTTAAGGTTGGCTTTTATGTTTGCTGGATTAAGGGGAATTAATCTTTGCTTATTGGT‑GCAGCTAAGGGATACGGGTTTTGATGTAGCCGGTTGTAATTCAGCGCATACATGGCGGTGATGACCATCAggg              <  2:917174/143‑1 (MQ=255)

                                                                                                                                    ccAACCTTAAGTTCTTAAGGTTGGCCTTTATGTTTGTTTGAGTAAGGGGAATTAATCTTTGCGTATTGGT‑GCAGCTAAGGGAGACGGGGTTTTATGTAGCCGGTTGTAATTCAGCGCATACATGGCGGTGATGAGCATCAggg              >  2:774588/1‑143 (MQ=255)

                                                                                                                                     cAACCTTAAGTTCTTAAGGTTCGCTTCTCCCCTTGATGCGGTGGGAGGAATTAATCTTTGCTTATTTGT‑GCACCTGAGGGATACGGGAGCTTATGTAGCCGGTTGTAATTCAGCGCATACAGGGCGGGGATGGCCATGAGGGt             >  2:153119/1‑143 (MQ=255)

                                                                                                                                     cAACCTTAAGAACTTAAGGTTGGCTTTTATGTTTGCTGGATTAAGGGGAATTAATCTTTGCTTATTGGT‑GCATCTAAGGGATACGGGTTTTTATGTAGCCGGTTGTAATTCAGCGCATACATGGCGGTGATGACCATCAGGGt             >  2:599278/1‑143 (MQ=255)

                                                                                                                                          ttAAGTTCTTAAGGTTGGCTTTTATGTTTGCTGGATTAAGGGGAATTAATCTTTGCTTATTGGT‑GCATCTAAGGGATACGGGTTTTTATGTAGCCGGTTGTAATTCAGCGCATACATGGCGGTGATGACCATCAGGGTGAGaa        >  1:525950/1‑143 (MQ=255)

                                                                                                                                           tgaggtCTTAAGGTTGGCTTTTATGTTTGCTGGATTAAGGGGAATTAATCTTTGCTTATCGGT‑GCATCTAAGGGATACGGGTTTTTATGTAGCCGGTTGTAATTCAGCGCATACATGGCGGTGATGACCATCAGGGTGACaaa       <  2:1110490/138‑1 (MQ=255)

                                                                                                                                           tAAGTTCTTAAGGTTGGCTTTTATGTTTGCTGGAGTAAGGGGAATTAATCTTTGCTTATTGGT‑GGATCTAAGGGATACGGGTTTTTATGTAGCCGGTTGTAATTCAGCGCATACATGGCGGTGATGACCATCAGGGTGACaaa       <  2:559942/143‑1 (MQ=255)

                                                                                                                                              gTTCTTAAGGTTGGCTTTTATGTTTGCTGGATTAAGGGGAATTAATCTTTGCTTATTGGT‑GCATCTAAGGGATACGGGTTTTTATGTAGCCGGTTGTAATTCAGCGCATACATGGCGGTGATGACCATCAGGGTGACAAATGa    <  1:947816/143‑1 (MQ=255)

                                                                                                                                              gTTCTTAAGGTTGGCTTTTATGTTTGCTGGATTAAGGGGAATTAATCGTTGCTTATTGGT‑GCATCTAAGGGATACGGGTTTTTATGTAGCCGGTTGTAATTCAGCGCATACATGGCGGTGATGACCATCAGGGTGACAAAtca    >  2:290518/1‑141 (MQ=255)

                                                                                                                                               ttCTTAAGGTTGGCTTTTATGTTTGCTGGATTAAGGGGAATTAATCTTTGCTTATTGGT‑GCATCTAAGGGATATGGGTTTTTATGTAGCCGGTTGTAATTCAGCGCATACATGGCGGTGATGACCATCAGGGTGACAAATGAc   <  1:1046129/143‑1 (MQ=255)

                                                                                                                                               ttCTTAAGGTTGGCTTTTATGTTTGCTGGAGTAAGGGGAATTAATCTTTGCTTATTGGT‑GCATCTAAGGGATACGGGTTTTTATGTAGCCGGTTGTAATTCAGCGCATACATGGCGGTGATGACCATCAGGGGGTCAACTGAc   >  2:61810/1‑143 (MQ=255)

                                                                                                                                                tCTTAAGGTTGGCTTTTATGTTTGCTGGATTAAGGGGAATTAATCTTTGCTTATTGGT‑GCATCTAAGGGATACGGGTTTTTATGTAGCCGGTTGTAATTCAGCGCATACATGGCGGTGATGACCATCAGGGTGACAAATGAcc  <  1:545002/143‑1 (MQ=255)

                                                                                                                                                tCTTAAGGTTGGCTTTTATGTTTGCTGGATTAAGGGGAATTAATCTTTGCTTATTGGT‑GCATCTAAGGGATACGGGTTTGTATGTAGCCGGTTGTAATTCAGCGCATACATGGCGGTGATGACCATCAGGGTGACAAATGAcc  <  2:160065/143‑1 (MQ=255)

GATAGAGATATAAAAAGCGGCCAACAATAGCCAGGCGAGTAAATTCAAAGAGGGTCTTTTTAGTAGGCGCTTCAACATCACGGTGTTTCCATCGAACAAAGTGCGCATATGCTCGCAAAGCAAA‑ATT‑AAGCCAACCTTAAGTTCTTAAGGTTGGCTTTTATGTTTGCTGGATTAAGGGGAATTAATCTTTGCTTATTGGT‑GCATCTAAGGGATACGGGTTTTTATGTAGCCGGTTGTAATTCAGCGCATACATGGCGGTGATGACCATCAGGGTGACAAATGACC  >  NC_000913/4335513‑4335797

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 13 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`