Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	569,161	A→C	9.5%	D87A (GAC→GCC) ‡	ybcK →	DLP12 prophage; putative recombinase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	569,161	0	A	C	9.5%	165.9 / 8.8	66	D87A (GAC→GCC) ‡	ybcK	DLP12 prophage; putative recombinase
Reads supporting (aligned to +/- strand):  ref base A (21/36);  new base C (2/4);  total (24/42)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.81e-01

CTTGATACCATAACATATGAAGATTTAGGATTAAGTGCATTCAAAGGAAAGCATGCACAATCAGGAGCTTTTTCGGAATTTTTAGATGCTATAGAGCATGGTTATATATTGCCAGGAACTACATTGTTAGTTGAAAGTCTGGACAGACTTTCAAGAGAAAAAGTCGGTGAAGCGATTGAACGTCTGAAATTGATTTTGAATCACGGTATTGATGTTATAACTCTTTGCGACAATACAGTCTATAATATTGACTCTTTGAATGAGCCATATTCATTAATAAAAGC  >  NC_000913/569019‑569302                                                                                                                                               |                                                                                                                                              cTTGATCCCATAACATATGAAGATTTAGGATTAAGTGCATTCAAAGGAAAGCATGCACAATCAGGAGCTTTTTCGGAATTTTTAGATGCTATAGAGCATGGTTATATATTGCCAGGAACTACATTGTTAGTTGAAAGTCTGGa                                                                                                                                               <  1:240340/143‑1 (MQ=255) cTTGATACCATAACATATGAAGATTTAGGATTAAGTGCATTCAAAGGAAAGCATGCACAATCAGGAGCTTTTTCGGAATTTTTAGATGCTATAGAGCATGGTTATATATTGCCAGGAACTACATTGTTAGTTGAAAGTCTGGa                                                                                                                                               <  1:1017044/143‑1 (MQ=255) cTTGATACCATAACATATGAAGATTTAGGATTAAGTGCATTCAAAGGAAAGCATGCACAATCAGGAGCTTTTTCGGAATTTTTAGATGCTATAGAGCATGGTTATATATTGCCAGGAACTACATTGTTAGTTGAAAGTCTGGa                                                                                                                                               <  1:456671/143‑1 (MQ=255) cTTGATACCATAACATATGAACATTTAGGATTAAGTGCATTCAAAGGAAAGCATGCACAATCAGGAGCTTTTTCGGAATTTTTAGATGCTATAGAGCATGGTTATATATTGCCAGGAACTACATTGTTAGTTGAAAGTCTGGa                                                                                                                                               <  2:814136/143‑1 (MQ=255)   tGATACCATAACAAACGCAGATTTAGGATTAAGTGCCTTCAAAGGAAAGCATGCACAATCAGGAGCTTTATAGGAAAGTTTAGATGCGATAGAGCAAGGTTATATATTGACCGGAACTACAAGGATAGATGAAGGTCTGGGCa                                                                                                                                             >  2:800882/1‑143 (MQ=255)    gATACCATAACATATGAAGATTTAGGATTAAGTGCATTCAAAGGAAAGCATGCACAATCAGGAGCTTTTTCGGAATTTTTAGATGCTATAGAGCATGGTTATATATTGCCAGGAACTACATTGTTAGTTGAAAGTCTGGACAg                                                                                                                                            <  1:776561/143‑1 (MQ=255)      tACCATAACATATGAAGATTTAGGATTAAGTGCATTCAAAGGAAAGCATGCACAATCAGGAGCTTTTTCGGAATTTTTAGATGCTATAGAGCATGGTTATATATTGCCAGGAACTACATTGTTAGTTGAAAGTCTGGACAGAc                                                                                                                                          <  2:243351/143‑1 (MQ=255)      tACCATAACATATGAAGATTTAGGATTAAGTGCATTCAAAGGAAAGCATGCACAATCAGGAGCTTTTTCGGAATTTTTAGATGCTATAGAGCATGGTTATATATTGCCAGGAACTACATTGTTAGTTGAAAGTCTGGACAGAc                                                                                                                                          <  1:965321/143‑1 (MQ=255)         cATAACATATGAAGATTTAGGATTAAGTGCATTGAAAGGAAAGCATGCACAATCAGGAGCTTTTTCGGAATTTTTAGATGCTATAGAGCATGGTTATATATTGCCAGGAACTACATTGTTAGTTGAAAGTCTGGACAGACttt                                                                                                                                       >  2:997278/1‑143 (MQ=255)         cATAACATATGAAGATTTAGGATTAAGTGCATTCAAAGGAAAGCATGCACAATCAGGAGCTTTTTCGGAATTTTTAGATGCTATAGAGCATGGTTATATATTGCCAGGAACTACATTGTTAGTTGAAAGTCTGGACAGACttt                                                                                                                                       <  2:820388/143‑1 (MQ=255)          aTAACATATGAAGATTTAGGATTAAGTGCATTCAAAGGAAAGCATGCACAATCAGGAGCTTTTTCGGAATTTTTAGATGCTATAGAGCATGGTTATATATTGCCAGGAACTACATTGTTAGTTGAAAGTCTGTCCAGACTTTc                                                                                                                                      <  1:121769/143‑1 (MQ=255)            aaCATATGAAGATTTAGGATTAAGTGCATTCAAAGGAAAGCATGCACAATCAGGAGCTTTTTCGGAATTTTTAGATGCTATAGAGCATGGTTATATATTGCCAGGAACTACATTGTTAGTTGAAAGTCTGTCCAGACTTTCaa                                                                                                                                    <  1:717621/143‑1 (MQ=255)            aaCATATGAAGATTTAGGATTAAGTGCATTCAAAGGAAAGCATGCACAATCAGGAGCTTTTTCGGAATTTTTAGATGCTATAGAGCATGGTTATATATTGCCAGGAACTACATTGTTAGTTGAAAGTCTGTCCAGACTTTCaa                                                                                                                                    <  1:417349/143‑1 (MQ=255)            aaCATAGGAAGATTGAGGATTAAGTGCATTCAAAGGAAAGCATGCACAATCAGGAGCTTTTTCGGAATTTTTAGATGCTATAGAGCATGGTTATATATTGCCAGGAACTACATTGTTAGTGGAAAGTCTGTCCAGACTTTCaa                                                                                                                                    <  2:135940/143‑1 (MQ=255)               atatGAAGATTTAGGATTAAGTGCATTCAAAGGAAAGCATGCACAATCAGGAGCTTTTTCGGAATTTTTAGATGCTATAGAGCATGGTTATATATTGCCAGGAACTACATTGTTAGTTGAAAGTCTGGACAGACTTTCAagag                                                                                                                                 >  2:8719/1‑143 (MQ=255)                      gATTTAGGATTAAGTGCATTCAAAGGAAAGCATGCACAATCAGGAGCTTTTTCGGAATTTTTAGATGCTATAGAGCATGGTTATATATTGCCAGGAACTACATTGTTAGTTGAAAGTCTGGACAGACTTTCAAGAGAAAAAGt                                                                                                                          <  2:586298/143‑1 (MQ=255)                        tttAGGATTAAGTGCATTCAAAGGAAAGCATGCACAATCAGGAGCTTTTTCGGAATTTTTAGATGCTATAGAGCATGGTTATATATTGTCAGGAACTACATTGTTAGTTGAAAGTCTGGACAGACTTTCAAGAGAAAAAGTCg                                                                                                                        <  1:668230/143‑1 (MQ=255)                        tttAGGATTAAGTGCATTCAAAGGAAAGCATGCACAATCAGGAGCTTTTTCGGAATTTTTAGATGCTATAGAGCATGGTTATATATTGCCAGGAACTACATTGTTAGTTGAAAGTCTGGACAGACTTTCAAGAGAAAAAGTCg                                                                                                                        <  2:1084042/143‑1 (MQ=255)                         ttAGGATTAAGTGCATTCAAAGGAAAGCATGCACAATCAGGAGCTTTTTCGGAATTTTTAGATGCTATAGAGCATGGTTATATATTGCCAGGAACTACATTGTTAGTTGAAAGTCTGGACAGACTTTCAAGAGAAAAAGTCgg                                                                                                                       >  1:50593/1‑143 (MQ=255)                          tAGGATGAAGTGCATTGAAAGGAAAGCATGCACAATCAGGAGCTTGTTCGCAATTTTTAGATGCTATAGAGCATGGTTATAGATTGCCAGGAACTACATTGTTAGGTGAAAGTCTGGACAGACTTTCAAGAGAAAAAGTCGGt                                                                                                                      <  2:648062/143‑1 (MQ=255)                                  aGTGCATTCAAAGGAAAGCATGCACAATCAGGAGCTTTTTCGGAATTTTTAGATGCTATAGAGCATGGTTATATATTGCCAGGAACTACATTGTTAGTTGAAAGTCTGGACAGACTTTCAAGAGAAAAAGTCGGTGAAGCGAt                                                                                                              <  1:978901/143‑1 (MQ=255)                                   gTGCATTCAAAGGGAAGCATGCACAATCAGGAGCTTTTTCGGAATTTTTAGATGCTATAGCGCGTGGCTCCAAACACCCAGGGGGTACTTTATTACGTTAAAGTCTGGACAGACTTTCAAGAGAAAAAGTCGGTGAAGCGAtt                                                                                                             <  1:595200/143‑1 (MQ=255)                                   gTGCATTCAAAGGAAAGCATGCACAATCAGGAGCTTTTTCGGAATTTTTAGATGCTATAGAGCATGGTTATATATTGCCAGGAACTACATTGTTAGTTGAAAGTCTGGACAGACTTTCAAGAGAAAAAGTCGGTGAAGCGAtt                                                                                                             <  1:189762/143‑1 (MQ=255)                                      cATTCAAAGGAAAGCATGCACAATCAGGAGCTTTTGCGGAATTTTTAGATGCTATAGAGCATGGTTATATAATGACAGGAACTACATTGTTAGTTGAAAGTCTGGACAGACTTTCAAGAGAAAAAGACGGTGACGCGATTGaa                                                                                                          >  2:493414/1‑143 (MQ=255)                                       aTTCAAAGGAATGCATGCACAATCAGGAGCTTTTTCGGAATTTTTAGTTGCTTTTGTGCGTGGTTGTATGTTGCCAGGAACTACATTGTTAGTTGAATGTCTGGACCGACTTGCAAGAGAAAAAGTCGGTGAAGCGATTGAAc                                                                                                         <  2:326477/143‑1 (MQ=255)                                               gAAAGCATGCACAATCAGGAGCTTTTTCGGAATTTTTAGATGCTTTGGACTTATGTGAGATATTGTGATTAACTACATTGTTAGTTGAAAGTCTGGACAGACTTTCAAGAGAAAAAGTCGGTGAAGCGATTGAACGTCTGaaa                                                                                                 <  2:567729/143‑1 (MQ=255)                                                aaaGCATGCACAATCAGGAGCTTTTTCGGAATTTTTAGCTGCTATAGAGCATGGCTATATATTGCCAGGAACTACATTGTAAGTTGAAAGTCTGGACAGACTTTCAAGAGAAAAAGTCGGGGAAGCGATTGAACGCCTGAAAt                                                                                                >  2:247014/1‑143 (MQ=255)                                                  aGCATGCAGAATCAGGAGCTTTTTCGGAATTTTTAGATGCTATAGAGCATGGTTATATATTGCCAGGAACTACATTGTTAGTTGAAAGTCTGGACAGACTTTCAAGAGAAAAAGTCGGTGAAGCGATTGAACGTCTGAAATTg                                                                                              <  1:1082066/143‑1 (MQ=255)                                                   gCATGCACAATCAGGAGCTTTTTCGGAATTTTTAGATGCTATAGAGCATGGTTATATATTGCCAGGAACTACATTGTTAGTTGAAAGTCTGGACAGACTTTCAAGAGAAAAAGTCGGTGAAGCGATTGAACGTCTGAAATTGa                                                                                             <  2:72628/143‑1 (MQ=255)                                                    cATGCACAATCAGGAGCTTTTTCGGAATTTTTAGATGCTATAGAGCATGGTTATATATTGCCAGGAACTACATTGTTAGTTGAAAGTCTGGACAGACTTTCAAGAGAAAAAGTCGGTGAAGCGATTGAACGTCTGAAATTGAt                                                                                            >  2:345916/1‑143 (MQ=255)                                                      ggCACAATCTGGAGCTTTTTCGGAATTTTTAGATCTTATAGAGCATTGTTATATATTGCCAGGAACTACATTGTTAGTTGAAAGTCTGGACAGACTTTCAAGAGAAAAAGTCGGTGAAGCGATTGAACGTCTGAAATTGAttt                                                                                          <  2:1015404/142‑1 (MQ=255)                                                          caATCCGGGGCTTTTTCGGAATTTTTAGATGCTATAGAGCATGGTTATATATTGCCAGGAACTACATTGTTAGTTGAAAGTCTGGACAGACTTTCAAGAGAAAAAGTCGGTGAAGCGATTGAACGTCTGAAATTGATTTTGaa                                                                                      <  1:468220/143‑1 (MQ=255)                                                              cAGGGGCGTTTTCGGAATTGTTAGATGCTATAGGGCATGGTTATATATTGCCAGGAACTACATTGTTAGTTGAAAGTCTGGACAGACTTTCAAGAGAAAAAGTCGGTGAAGCGATTGAACGTCTGAAATTGATTTTGAATCAc                                                                                  <  2:368316/143‑1 (MQ=255)                                                               aGGAGCTTTTTCGGAATTTGTAGATGCTAGAGAGCATGGTTATATATTGCCAGGAACTACATTGTTAGTTGAAAGTCTGGACAGACTTTCAAGAGAAAAAGTCGGTGAAGCGATTGAACGTCTGAAATTGATTTTGAATCACg                                                                                 <  1:1114800/143‑1 (MQ=255)                                                               aGGAGCTTTGTCGGAATTTCTAGATGCTATAGAGCATGGTTATACATTGCCAGGAACTCCACAGCTACTGGAAAGTCTGGACAGACTTTCAAGAGAAAAAGTCGGTGAAGTGATTGAACGTCTGAAATTGATTTTGAATCACg                                                                                 <  2:695234/143‑1 (MQ=255)                                                               aGGAGCGTTTTCGGAATTTTTAGATGCTATAGAGCATGGTTATATATTGCCAGGAACTACATTGTTAGTTGAAAGTCTGGACAGACTTTCAAGAGAAAAAGTCGGTGAAGCGATTGAACGTCTGAAATTGATTTTGAATCACg                                                                                 <  2:1013841/143‑1 (MQ=255)                                                                       tttCGGAATTTTTAGATGCTATAGAGCATGGTTATATATTGCCAGGAACTACATTGTTAGTTGAAAGTCTGGACAGACTTTCAAGAGAAAAAGTCGGTGAAGCGATTGAACGTCTGAAATTGATTTTGAATCACGGTATTGAt                                                                         <  2:541155/143‑1 (MQ=255)                                                                       tttCGGAATTTTTAGATGCTATAGAGCATGGTTATATATTGCCAGGAACTACATTGTTAGTTGAAAGTCTGGACAGACTTTCAAGAGAAAAAGTCGGTGAAGCGATTGAACGTCTGAAATTGATTTTGAATCACGGTATTGAt                                                                         <  1:901937/143‑1 (MQ=255)                                                                        ttCGGAATTTTTAGATGCTATAGAGCATGGTCATATATTGCCAGGAACTACATTGTTAGTTGAAAGTCTGGACAGACTTTCAAGAGAAAAAGTCGGTGAAGCGATTGAACGTCTGAAATTGATTTTGAATCACGGTATTGATg                                                                        <  1:327088/143‑1 (MQ=255)                                                                            gtaTTTTTAGATGCGATAGAGGATGGTTCTATATTGGCAGGAACTACATTGTTAGTTGAAAGTCTGGGCAGACTTGCAAGAGACAAAGTCTGTGAAGCGATTGAACGTCTGAAATTGATTTTGAATCACGGTATTGATGTtat                                                                    <  2:814567/141‑1 (MQ=255)                                                                            gAATTTTTAGATGCTATAGAGCATGGTTATATATTGCCAGGAACTACATTGTTAGCCACTATTCGGGACAGACTTTCAAGAGAAAAAGTCGGTGAAGCGATTGAACGTCTGAAATTGATTTTGAATCACGGTATTGATGTtat                                                                    <  1:1271033/143‑1 (MQ=255)                                                                              aTTTTTAGATGGTATAGAGCATGGTTATATATTGCCAGGAACTACATTGTTAGTTGAAAGTCTGGACAGACTTTCAAGAGAAAAAGTCGGTGAAGCGATTGAACGTCTGAAATTGATTTTGAATCACGGTATTGATGTTATaa                                                                  <  1:627639/143‑1 (MQ=255)                                                                              aTTTTTAGATGCAATAGAGCATGGTTATATATTGCCAGGAACTACATTGTTAGTTGAAAGTCTGGACAGACTTTCAAGAGAAAAAGTGGGTGAAGCGATTGAACGACTGAAATTGATTAGTAATCACGGTATTGATGtaataa                                                                  >  2:1198451/1‑143 (MQ=255)                                                                                    aGATGCTATAGAGCATGGTTATATATTGCCAGGAACTACATTGTTAGTTGAAAGTCTGGACAGACTTTCAAGAGAAAAAGTCGGTGAAGCGATTGAACGTCTGAAATTGATTTTGAATCACGGTATTGATGTTATAACTCttt                                                            <  1:664206/143‑1 (MQ=255)                                                                                    aGATGCTATAGAGCATGGTTATATATTGCCAGGAACTACATTGTTAGTTGAAAGTCTGGACAGACTTTCAAGAGAAAAAGTCGGTGAAGCGATTGAACGTCTGAAATTGATTTTGAATCACGGTATTGATGTTATAACTCttt                                                            >  1:381682/1‑143 (MQ=255)                                                                                    aGATGCTAGAGAGCATGGTTATATATTGCCAGGAACTACATTGTTAGTTGAAAGTCTGGACAGCCAATCAAGAGAAAAAGTCGGTGAAGCGATTGAACGTCTGAAATTGATTTTGAATCACGGTATTGATGTTATAACTCttt                                                            <  2:1114995/143‑1 (MQ=255)                                                                                           ataGAGCATGGTTATATATTGCCAGGAACTACATTGTTAGTTGAAAGTCTGGACAGACTTTCAAGAGAAAAAGTCGGTGCAGCGATTGAACGTCTGAAATTGAATTTCAATCCCGGGGTTGATGTTATAACTCTTTGCGACaa                                                     >  1:531852/1‑143 (MQ=255)                                                                                                 cATGGTTATATATTGCCAGGAACTACATTGTTAGTTGAAAGTCTGGACAGACTTTCAAGAGAAAAAGTCGGTGAAGCGATTGAACGTCTGAAATTGATTTTGAATCACGGTATTGATGTTATAACTCTTTGCGACAATACAGt                                               <  2:1079364/143‑1 (MQ=255)                                                                                                    ggTTATATATTGCCAGGAACTACATTGTTAGTTGAAAGTCTGGACAGACTTTCAAGAGAAAAAGTCGGTGAAGCGATTGAACGTCTGAAATTGATTTTGAATCACGGTATTGATGTTATAACTCTTTGCGACAATACAGTCta                                            <  1:566485/143‑1 (MQ=255)                                                                                                      ttATATATTGCCAGGAACTACATTGTTAGTTGAAAGTCTGGACAGACTTTCAAGAGAAAAAGTCGGTGAAGCGATTGAACGTCTGAAATTGATTTTGAATCACGGTATTGATGTTATAACTCTTTGCGACAATACAGTCtata                                          >  1:1221806/1‑143 (MQ=255)                                                                                                         tatatTGCCAGGAACTACATTGTTAGTTGAAAGTCTGGACAGACTTTCAAGAGAAAAAGTCGGTGAAGCGATTGAACGTCTGAAATTGATTTTGAATCACGGAATTGATGTGATAACTCTTTGCGACAATACAGTCTataata                                       >  2:1176070/1‑143 (MQ=255)                                                                                                           tatTGCCAGGAACTACATTGTTAGTTGAAAGTCTGGACAGACTTTCAAGAGAAAAAGTCGGTGAAGCGATTGAACGTCTGAAATTGATTTTGAATCACGGTATTGATGTTATAACTCTTTGCGACAATACAGTCTCTAATAtt                                     >  1:1030834/1‑143 (MQ=255)                                                                                                           agttGCCATGGACTACATTGGTAGTTGAAAGTCTGGACAGACTTTCAAGAGAAGAAGTCGGTGAAGCGATTGAACGTCTGAAATTGATTTTGAATCACGGTATTGATGTTATAACTCTTTGCGACAATACAGTCTATAATAtt                                     <  2:752137/141‑1 (MQ=255)                                                                                                                        tACATTGTTAGTTGAAAGTCTGGACAGACTTTCAAGAGAAAAAGTCGGTGAAGCGATTGAACGTCTGAAATTGATTTTGAATCACGGTATTGATGTTATAACTCTTTGCGACAATACAGCCTATGATATTGTCTTTTTCaaga                        >  1:583365/1‑141 (MQ=255)                                                                                                                          cATTGTTAGTTGAAAGTCTGGACAGACTTTCAAGAGAAAAAGTCGGTGAAGCGATTGAACGTCTGAAATTGATTTTGAATCACGGTATTGATGTTATAACTCTTTGCGACAATACAGTCTATAATATTGACTCTTTGATTGAg                      >  1:1104720/1‑143 (MQ=255)                                                                                                                               ttAGTTGAAAGTCTGGACAGACTTTCAAGAGACAACGACGGCGAAGCGAATAAACGTCTGAAATTGATTTTGAATCACGGTATTGATGTTATAACTCTTTGCGACAATACAGACTATACTATTGACTCTTTGAATGCGCCata                 >  2:390368/1‑143 (MQ=255)                                                                                                                               ttAGTTGAAAGTCTGGACAGACTTTCAAGAGAAAAAGTCGGTGAAGTGATTGAACGTCTGAAATTGATTTTGAATCACGGTATTGATGTTATAACTCTTTGCGACAATACAGTCTATAATATTGACTCTTTGAATGAGCCata                 <  1:8719/143‑1 (MQ=255)                                                                                                                               ttAGTTGAAAGTCTGGACAGACTTTCAAGAGAAAAAGTCGGTGAAGCGATTGAACGTCTGAAATTGATTTTGAATCACCGTATTGATGTTATAACTCTTTGCGACAATACAGTCTATAATATTGACTCTTTGAATGAGCCata                 >  2:1031268/1‑143 (MQ=255)                                                                                                                                  gTTGAAAGTCTGGACAGACTTTCAAGAGAAAAAGTCGGTGAAGCGATTGAAGGTCTGAAATTGATTTTGAATCACGGTATTGATGTTATAACTCTTTGCGACAATGCAGTCTATAATATTGACTCTTTGCATGAGCACTATTc              >  1:521648/1‑143 (MQ=255)                                                                                                                                   ttGAAAGTCTGTCCAGACTTTCAAGAGAAAAAGTCGGTGAAGCGATTGAACGTCTGAAATTGATTTTGAATCACGGTATTGATGTTATAGATCTTTGCGACAATACAGCATATAATATTGACTCTTTGACTGAGCCATATTCa             >  2:506207/1‑143 (MQ=255)                                                                                                                                   ttGAAAGTCTGGACAGACTTTCAAGAGAAAAAGTCGGTGAAGCGATTGAACGTCTGAAATTGATTTTGAATCACGGTATTGATGTTATAACTCTTTGCGACAATACAGTCTAGAATATTGACTCTTTGAGTGGGCCCTCTTct             >  1:107732/1‑142 (MQ=255)                                                                                                                                    tGAAAGTCTGGACAGACTTTCAAGAGAAAAAGTCGGTGAAGCGATTGAACGTCTGAAATTGATTTTGAATCACGGTATTGATGTTATAACTCTTTGCGACAATACAGTCTATAATATTGACTCTTTGAATGAGCCATATTCAt            <  1:7223/143‑1 (MQ=255)                                                                                                                                    tGAAACTCTAAACAGACTTTCAAGAGAAAAAGTCGGTCAAGCGACTGAACCCCTAAAATTGATTTTGAATCACTGTATTGATGTTATATCTCTTTTCGACAATACAGTCTATAATATTGACTCTTTAAATGAGCCATATTCAt            <  1:1222091/143‑1 (MQ=255)                                                                                                                                     gAAAGTCTGTCCAGACTTTCAAGAGAAACAGTCGGTGAAGCGATTGAACGTCTGAAATTGATGATGAATCACGGTATTGATGTTATAACTCTTTGCGACAATACAGTCTATAATATTGACTCTTTGAATGAGCCATATTCAtt           >  2:1183895/1‑143 (MQ=255)                                                                                                                                      aaaGTCTGGTCAGACTTTCAAGAGAGATCGGCGGTGAAGCGATTGAACGTCTGAAATTGATTTTGAATCACTGTATTGATGTTATAACTCTTTGCGACATTACAGTCTATAATATTGACTCTTTGAATGAGCCATATTCATta          <  2:614142/143‑1 (MQ=255)                                                                                                                                      aaaGTCTGGACAGACTTTCAAGAGAAAAAGTCGGTGAAGCGATTGAACGTCTGAAATTGATTTTGAATCACGGTATTGATGTTATAACTCTTTGCGACAATACAGTCTATAATATTGACTCTTTGAATGAGCCATATTCATta          <  1:1001951/143‑1 (MQ=255)                                                                                                                                      aaaGTCTGGACAGACTTTCAAGAGAAAAAGTCGGTGAAGCGATTGAACGTCTGAAATTGATTTTGAATCACGGTATAGATGTTATAACTCTTTGCGACAATACAGTCTATAATATTGACTCTTTGAAAGAGCAATATTCATta          >  2:406608/1‑143 (MQ=255)                                                                                                                                         gTCTGGACAGACTTTCAAGAGAAAAAGTCGGTGAAGCGATTGAACGTCTGAAATTGATTTTGAATCACGGTATTGATGTTATAACTCTTTGCGACAATACAGTCTATAATATTGACTCTTTGAATGAGCCATATTCATtaata       >  2:1006221/1‑143 (MQ=255)                                                                                                                                            tGGACGGACTATCAAGAGAAAAAGTGGGTGAGGCGATTGAACGTCGGAAATTGATTTGGAATCACGGGAGTGATGTTATAACTCTTTGCGACAATACAGTCTATAATATTGACTCTGTGAATGAGCCATATTCATTAATaaaa    <  2:651310/143‑1 (MQ=255)                                                                                                                                              gACAGACTTTCAAGAGAAAAAGTCGGTGAAGCGATTGAACGTCTGAAATTGATTTTGAATCACGGTATTGATGTTATAACTCTTTGCGACAATACAGTCTATAATATTGACTCTTTGAATGAGCCATATTCATTAATAAAAGc  >  1:842240/1‑143 (MQ=255)                                                                                                                                               |                                                                                                                                              CTTGATACCATAACATATGAAGATTTAGGATTAAGTGCATTCAAAGGAAAGCATGCACAATCAGGAGCTTTTTCGGAATTTTTAGATGCTATAGAGCATGGTTATATATTGCCAGGAACTACATTGTTAGTTGAAAGTCTGGACAGACTTTCAAGAGAAAAAGTCGGTGAAGCGATTGAACGTCTGAAATTGATTTTGAATCACGGTATTGATGTTATAACTCTTTGCGACAATACAGTCTATAATATTGACTCTTTGAATGAGCCATATTCATTAATAAAAGC  >  NC_000913/569019‑569302

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑