| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_000913 | 699,556 | C→T | 14.8% | intergenic (‑379/+18) | asnB ← / ← umpH | asparagine synthetase B/UMP phosphatase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 699,556 | 0 | C | T | 14.8% | 100.7 / 9.9 | 47 | intergenic (‑379/+18) | asnB/umpH | asparagine synthetase B/UMP phosphatase |
| Reads supporting (aligned to +/- strand): ref base C (23/17); new base T (2/5); total (25/22) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.28e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.23e-01 | |||||||||||
GATTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAAGCATGAAAATTCAATATATTGCAGGAGCTGCGTAGGCCTGATAAGCGTAGCGCATCAGGCAGTTTGGCGTTTGTCATCAGAGCCAACCACGTCCGCAGACGTGGTTGCTATTCAGATAACGTCGATTTCAGCGACTGACGGGTAAATCCAGCTGGGGCGGAAAGGCATACTGTCGATATCGTCGAGCGACGAAACACCAGAAAGCACCAGAATCGTCTCCAGACCTGCCTGGAA > NC_000913/699414‑699696 | gATTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAAGCATGAAAATTCAATATATTGCAGGAGCTGCGTAGGCCTGATAAGCGTAGCGCATCAGGCAGTTTGGCGTTTGTCATCAGAGCCACCCACGTcc > 1:628452/1‑143 (MQ=255) aTTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAAGCATGAAAATTCAATATATTGCAGGAGCTGCGTAGGCCTGATAAGCGTAGCGCATCAGGCAGTTTGGCGTTTGTCATCAGAGCCAACCACGTCCg > 2:395997/1‑143 (MQ=255) ttGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAAGCATGAAAATTCAATATATTGCAGGAGCTGCGTAGGCCTGATAAGCGTAGCGCATCAGGCAGTTTGGCGTTTGTCATCAGAGCCAACCACGTCCGCa > 1:774998/1‑143 (MQ=255) cATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAAGCATGAAAATTCAATATATTGCAGGAGCTGCGTAGGCCTGATAAGCGTAGCGCATCAGGCAGTTTGGCGTTTGTCATCAGAGCCAACCACGTCCGCAGACGt < 2:109460/143‑1 (MQ=255) ccGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAAGCATGAAAATTCAATATATTGCAGGAGCTGCGTAGGCCTGATAAGCGTAGCGCATCAGGCAGTTTGGCGTTTGTCATCAGAGCCAACCACGTCTGCGGACGTGGtt < 2:346631/143‑1 (MQ=255) ccGGACGCGGCGTGAACGCCTTAGCCGGCCTACAAAAGCATGAAAATTCAATATATTGCAGGAGCTGCGTAGGCCTGATAAGCGTAGCGCATCAGGCAGTTTGGCGTTTGTCATCAGAGCCAACCACGTCTGCGGACGTGGtt < 1:31297/143‑1 (MQ=255) cgcctGCGGCGTGAACTCCTTACCCGGCCTACAAAACCATGAAAATTCAATATATTGCAGGAGCTGCGTAGGCCTGATAAGCGTAGCGCATCAGGCAGTTTGGCGTTTGTCATCAGAGCCAACCACGTCTGCGGACGTGGTTg < 1:894930/139‑1 (MQ=21) cGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAAGCATGAAAATTCACTATATTGCAGGAGCTGCGTAGGCCTGATAAGCGTAGCGCATCAGGCAGTTTGGCTTTTGTCATCAGAGCCAACCACGTCTGCGGACGTGGTTg < 1:253966/143‑1 (MQ=255) cGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAAGCATGAAAATTCAATATATTGCAGGAGCTGCGTAGGCCTGATAAGCGTAGCGCATCAGGCAGTTTGGCGTTTGTCATCAGAGCCAACCACGTCCGCAGACGTGGTTg > 1:614353/1‑143 (MQ=255) cGGATCGGGCGTGATCGCCTTATCCGGCCTACAAAAGCATGAAAATTCAATATATTGCAGGAGCTGCGTAGGCCTGATAAGCGTAGCGCATCAGGCAGTTTGGCGTTTGTCATCAGAGCCAACCACGTCCGCAGACGTGGTTg < 2:948329/143‑1 (MQ=255) cGGATACGGCGTGAACACCTTATCCGGCATACAAAAGCATGAAAACTCAAAACATAGCAGGAGCTGCGTAGGCCTGATAATCGAAGAGCAACAGACAGTTTGGCGTTTGACATCAGAGCCAACCACGTCCGCAGACGAggaca > 2:1089052/1‑140 (MQ=255) ggATGCGGCGTGAACGCCTTATCCGGCCTACAAAAGCATGAAAATTCAATATATTGCAGGAGCTGCGTAGGCCTGATAAGCGTAGCGCATCAGGCAGTTTGGCGTTTGTCATCAGAGCCAACCACGTCTGCGGACGTGGTTgg < 2:541515/143‑2 (MQ=255) cggcgTGAACGCCTTATCCGGCCTACAAAAGCATGACAATTCAATATATTGCAGGAGCTGCGTAGGCCTGATAAGCGTAGCGCATCAGGCAGTTTGGCGTTTGTCATCAGAGCCAACCACGTCCGCAGACGTGGTTGCTATTc > 1:826741/1‑143 (MQ=255) cggcgTGAACGCCTTATCCGGCCTACAAAAGCATGAAAATTCAATATATTGCAGGAGCTGCGTAGGCCTGATAAGCGTAGCGCATCAGGCAGTTTGGCGTTTGTCATCAGAGCCAACCACGTCCGCAGACGTGGTTGCTATTc > 1:764743/1‑143 (MQ=255) ggcgTGAACGCCTTATCCGGCCTACAAAAGCATGAAAATTCAATATATTGCAGGAGCTGCGTAGGCCTGATAAGCGTAGCGCATCAGGCAGTTTGGCGTTTGTCATCAGAGCCACCCAAGTGTGCTGTTTTGGTTGCTATTCa > 1:1199207/1‑143 (MQ=21) cgTGACCGCTTTCGCCGGCCTACAAAAGCATGAAAATTCAATATATTGCAGGAGCTGCGTAGGCCTGATAAGCGTAGCGCATCAGGCAGTTTGGCGTTTGTCATCAGAGCCAACCACGTCCGCAGACGTGGTTGCTATTCAGa < 2:422474/143‑1 (MQ=255) cgTGAACGCCTTATCCGGCCTACAAAAGCATGAAAATTCAATATATTGCAGGAGCTGCGAAGGCCTGATAAGCGTAGCGCATCAGGCAGTTTGGCGTTTGTCATCAGAGCCAACCACGTCCGCAGACGTGCGTGCTCTTCAGa > 2:787659/1‑143 (MQ=255) cTTATCCGGCCTACAAATGCATGAAAATTCAATATATTGCAGGAGCTGCGTAGGCCTGATAAGCGTAGCGCTTCAGGCAGTTTGGCGTTTGTCATCAGAGCCAACCACGTCCGCAGACGTGGTTGCTATTCAGATAACGTCGa < 2:800801/143‑1 (MQ=255) aTCCGGCCTACAAAAGCATGAAAATTCAATATATTGCAGGAGCTGCGTAGGCCTGATAAGCGTAGCGCATCAGGCAGTTTGGCGTTTGTCATCAGAGCCAACCACGTCCGCAGACGTGGTTGCTATTCAGATAACGTCGAttt < 1:843273/143‑1 (MQ=255) aCAAAAGCATGAAAATTCAATATATTGCAGGAGCTGCGTAGGCCTGATAAGCGTAGCGCATCAGGCAGTTTGGCGTTTGTCATCAGAGCCAACCACGTCCGCAGACGTGGTTGCTATTCCGATAACGTCGATTTTAGCGAcag > 1:583041/1‑141 (MQ=255) aaGCATGAAAATTCAATATATTGCAGGAGCTGCGTAGGCCTGATAAGCGTAGCGCATCAGGCAGTTTGGCGTTTGTCATCAGAGCCAACCACGTCCGCAGACGTGGTTGCTATTCAGATAACGTCGATTTCAGCGACTGACgg > 1:992389/1‑143 (MQ=255) aTTCAATATATTGCAGGAGCTGCGTAGGCCTGATAAGCGTAGCGCATCAGGCAGTTTGGCGTTTGTCATCAGAGCCAACCACGTCCGCAGACGTGGTTGCTATTCAGATAACGTCGATTTCAGCGACTGACGGGTAAATCCAg < 1:444363/143‑1 (MQ=255) tatatTGCAGGAGCTGCGTAGGCCTGATAAGCGTAGCGCATCAGGCAGTTTGGCGTTTGTCATCAGAGCCAACCACGTCCGCAGACGTGGTTGCTATTCAGATAACGTCGATTTCAGCGACTGACGGGTAAATCCAGCGgggg > 2:391853/1‑143 (MQ=255) cAGGAGCTGCGTAGGCCTGATAAGCGTAGCGCATCAGGCAGTTTGGCGTTTGTCATCAGAGCCAACCACGTCCGCAGACGTGGTTGCTATTCAGATAACGTCGATTTCAGCGACTGACGGGTAAATCCAGCTGGGGCGGAAAg < 1:636880/143‑1 (MQ=255) aGCTGCGTAGGCCTGATAAGCGTAGCGCATCAGGCAGTTTGGCGTTTGTCATCAGAGCCAACCACGTCCGCAGACGTGGTTGCTATTCAGATAACGTCGATTTCAGCGACTGACGGGTCAATCCAGCTGGGGCGGACAGGCAt > 1:45548/1‑143 (MQ=255) gCGTAGGCCTGATAAGCGTAGCGCATCAGGCAGTTTGGCGTTTGTCATCAGAGCCAACCACGTCCGCAGACGTGGTTGCTATTCAGATAACGTCGATTTCAGCGACTGACGGGTAAATCCAGCTGGGGCGGAAAGGCATACTg > 2:56416/1‑143 (MQ=255) gCGTAGGCCTGATAAGCGTAGCGCATCAGGCAGTTTGGCGTTTGTCATCAGAGCCAACCACGTCCGCAGACGTGGTTGCTATTCAGATAACGTCGATTTCAGCGACTGACGGGCAAATCCAGCTGGGCCGGACAGGCATACtc > 1:950857/1‑142 (MQ=255) gTAGGCCTGATAAGCGTAGCGCATCAGGCAGTTTGGCGTTTGTCATCAGTGAATAACTAGTACGATGAAGTGGTTGCTATTCAGATAACGTCGATTTCAGCGTCAAACGAGTATATCCAGCTGGGGCGGAAAGGCATACTGTc < 2:23586/143‑1 (MQ=21) aGGCCTGATAAGCGTAGCGCATCAGGCAGTTTGGCGTTTGTCATCAGAGCCAACCACGTCCGCAGACGTGGTTGCTATTCAGATAACGTCGATTTCAGCGACTGACGGGTAAATCCAGCTGGGGCGGAAAGGCATACTGTCGa < 1:122969/143‑1 (MQ=255) ccTGATAAGCGTAGCGCATCAGGCAGTTTGGCGTTTGTCATCAGAGCCAACCACGTCCGCAAACGTGGGTGCTATTCAGATAACGTCGATTTCAGCGACTGACGGGTAAATCCAGCTGGGGCGGACAGGCGTACTGTCGatat > 1:256916/1‑143 (MQ=255) cTGATAAGCGTAGCGCATCAGGCAGTTTGGCGTTTGTCATCAGCGCCAACCACGTCCGCAGACGTGGTTGCTATTCAGATAACGTCGCTTTCAGCGACTGACGGGTAAATCCAGCTGGGGCGGAAAGGCATACTGTCGATAtc > 2:843405/1‑143 (MQ=255) gATAAGCGTAGCGCATCAGGCAGTTTGGCGTTTGTCATCAGAGCCAACCACGTCCGCAGACGTGGTTGCTATTCAGATAACGTCGATTTCAGCGACTGACGGGTAAATCCAGCTGGGGCGGAAAGGCATACTGTCGATAtcgt < 1:240391/143‑1 (MQ=255) gTAGCGCAGCAGGCAGTTTGGCGTTTGTCATCAGAGCCAACCACGTCCGCAGACGTGGTTGCTATTCAGATAACGTCGATTTCAGCGACTGACGGGTAAATCCAGCTGGGGCGGAAAGGCATACTGTCGATATCGTCGAGcga < 2:617430/143‑1 (MQ=255) aGCGCATCAGGCAGTTTGGCGTTTGTCATCAGAGCCAACCACGTCCGCAGACGTGGTTGCTATTCAGATAACGTCGATTTCAGCGACTGACGGGTAAATCCAGCTGGGGCGGAAAGGCATACTGTCGATATCGTCGAGcgacg > 2:796685/1‑143 (MQ=255) cATCAGGCAGTTTGGCGTTTGTCATCAGAGCCAACCACGTCCGCAGACGTGGTTGCTATTCAGATAACGTCGATTTCAGCGACTGACGGGTAAATCCAGCTGGGGCGGAAAGGCATACTGTCGATATCGTCGAGCGACGAAac > 1:120984/1‑143 (MQ=255) gCAGTTTGGCGTTTGTCATCAGAGCCAACCACGTCCGCAGACGTGGTTGCTATTCAGATAACGTCGATTTCAGCGACTGACGGGTAAATCCAGCTGGGGCGGAAAGGCATACTGTCGATATCGTCGAGCGACGAAACACCAGa > 1:145700/1‑143 (MQ=255) aGTTTGGCGTTTGGCATCAGAGCCAACCACGTCCGCAGAGGTGGTTGCTATTCAGATAACGTCGATTTCAGCGACTGACGGGTAAATCCAGCTGGGGCGGAAAGGCATACTGTCGATATCGTCGAGCGACGAAACACCAGaaa < 2:91041/143‑1 (MQ=255) tttGGCGTTTGTCATCAGAGCCAACCACGTCCGCAGACGTGGTTGCTATTCAGATAACGTCGATTTCAGCGACTGACGGGTAAATCCAGCTGGGGCGGAAAGGCATACTGTCGATATCGTCGAGCGACGAAACACCAGAAAGc > 1:1072093/1‑143 (MQ=255) tGTCATCAGAGCCAACCACGGCCGCAGACGTGGGTGCTATTCAGAGAACGTCGATTTCAGCGACTGACGGGTAAATCCAGCTGGGGCGGAAAGGCATACTGTCGATATCGTCGAGCGACGAAACACCAGAAAGCACCAGAATc < 1:239699/143‑1 (MQ=255) tcatcaGAGCCAACCACGTCCGCAGACGTGGTTGCTATTCAGATAACGTCGATTTCAGCGACTGACGGGTAAATCCAGCTGGGGCGGAAAGGCATACTGTCGATATCGTCGAGCGACGAAACACCAGAAAGCACCAGAATCGt < 1:395997/143‑1 (MQ=255) tcaGAGCCAACCACGTCCGTAGACGTGGTTGCTATTCAGATAACGTCGATTTCAGCGACTGACGGGTAAATCCAGCTGGGGCGGAAAGGCATACTGTCGATATCGTCGAGCGACGAAACACCAGCACGCACCAGAGTCGtctc > 1:622584/1‑143 (MQ=255) agagGCAACCACGCCCGCCGGTGTGGCATCTGGTCACAGAACGCCGATGTCAGCGGCTGACGGGTAAATCCCGCTCGGGCGGAAAGGCAGACTGTCGATATCGTCGAGCGACGAAACACCAGAAAGCACCAGAATCGTCTCCa < 1:1139377/143‑1 (MQ=255) gagCCAACCACGTCCGCAGACGTGGTTGCTATTCAGATAACGTCGATTTCAGCGACTGACGGGTAAATCCAGCTGGGGCGGAAAGGCATACTGTCGATATCGTCGAGCGACGAAACACCAGAAAGCACCAGAATCGTCTCCAg < 1:621044/143‑1 (MQ=255) aaCCACGTCTGCGGACGTGGTTGCTATTCAGATAACGTCGATTTCAGCGACTGACGGGTAAATCCAGCTGGGGCGGAAAGGCATACTGTCGATATCGTCGAGCGACGAAACACCAGAAAGCACCAGACTCGTCCCCAGAcctg > 2:597241/1‑143 (MQ=255) aaCCACGTCCGCAGACGTGGTTGCTATTCAGATAACGTCGATTTCAGCGACTGACGGGTAAATCCAGCTGGGGCGGAAAGGCATACTGTCGATATCGTCGAGCGACGAAACACCAGAAAGCACCAGAATCGTCTCCAGAcctg > 1:1060009/1‑143 (MQ=255) tCCGCAGACGTGGTTGCTATTCAGATAACGTCGATTTCAGCGACTGACGTGTAAATCCAGCTGGGGCGGAAAGGCATACTGTCGATATCGTCGAGCGACGAAACACCAGAAAGCACCAGAATCGTCTCCAGACCTGCCTGGaa < 2:614353/143‑1 (MQ=255) tCCGCAGACGTGGTTGCTATTCAGATAACGTCGATTTCAGCGACTGACGGGTAAATCCAGCTGGGGCGGTAAGTCATACTGTCGATATCGTCGAGAGACGAAACACCATAAAGCACCAGAATCTTCTCCAGATCTGCCTCGaa > 1:103257/1‑143 (MQ=255) tCCGCAGACGTGGTTGCTATTCAGATAACGTCGATTTCAGCGACTGACGGGTAAATCCAGCTGGGGCGGAAAGGCATACTGTCGATATCGTCGAGCGACGAAACACCAGAAAGCACCAGAATCGTCTCCAGACCTGCCTGGaa > 1:783822/1‑143 (MQ=255) | GATTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAAGCATGAAAATTCAATATATTGCAGGAGCTGCGTAGGCCTGATAAGCGTAGCGCATCAGGCAGTTTGGCGTTTGTCATCAGAGCCAACCACGTCCGCAGACGTGGTTGCTATTCAGATAACGTCGATTTCAGCGACTGACGGGTAAATCCAGCTGGGGCGGAAAGGCATACTGTCGATATCGTCGAGCGACGAAACACCAGAAAGCACCAGAATCGTCTCCAGACCTGCCTGGAA > NC_000913/699414‑699696 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |