| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_000913 | 770,679 | G→T | 11.0% | intergenic (+68/‑779) | mngB → / → cydA | alpha‑mannosidase/cytochrome bd‑I ubiquinol oxidase subunit I |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 770,679 | 0 | G | T | 11.0% | 131.1 / 9.9 | 55 | intergenic (+68/‑779) | mngB/cydA | alpha‑mannosidase/cytochrome bd‑I ubiquinol oxidase subunit I |
| Reads supporting (aligned to +/- strand): ref base G (19/30); new base T (3/3); total (22/33) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.74e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.20e-01 | |||||||||||
GAAAATCAAGGTTCAAATCTATCGGGGCCTTTTTTACCCGGCCAGTCACGGACGTTCAGTTACCGGCT‑TGCCTGAATAGCAATCAAACCGAAGCCACATATGCGCGGCCAGATTGTTGACAAAGGGCGCTTTGTTCATGCCGGATACGGCATGAACGCTTTATTCGGTCTACAAAAGCAGGCAAATTCAATATATTGCAGAGATGATGTAGACACTGGCAAGCGTAGCGCATCAGGTAATTTTGCGTTTATCTTCACTCTCAAGCCACGTATATGTGGCTTTAT > NC_000913/770537‑770820 | gAAAATCAAGGTTCAAATCTATCGGGGCCTTTTTTACCCGGCCAGTCACGGACGTTCAGTTACCGGCT‑TGCGTGAATAGCAATCAAACCGAAGCCACATATGCGCGGCCAGATTGTTGACAAAGGGCGCTTTGTTCATGCCgg > 2:824500/1‑143 (MQ=255) aaaaTCAAGGTTCAAATCTATCGGGGCCTTTTTTACCCGGCCAGTCACGGACGTTCAGTTACCGGCT‑TGCCTGAATAGCAATCAAACCGAAGCCACATATGCGCGGCCAGCTTGTTGACAAAGGGCGCTTTGTTCATGTCGGa > 1:509398/1‑143 (MQ=255) aTCAAGGTTCAAATCTATCGGGGCCTTTTTTACCCGGCCAGTCACGGACGTTCAGTTACCGGCT‑TGCCTGAATAGCAATCAAACCGAAGCCACATATGCGCGGCCAGATTGTTGACAAAGGGCGCTTTGTTCATGCCGGATAc < 1:1189004/143‑1 (MQ=255) tCAAGGTTCAAATCTATCGGGGCCTTTTTTACCCGGCCAGTCACGGACGTTCAGTTACCGGCT‑TGCCTGAATAGCAATCAAACCGAAGCCACATATGCGCGGCCAGATTGTTGACAGAGGGCGCTTTGTTCATGCCGGATACg > 1:892586/1‑143 (MQ=255) cAAGGTTCAAATCTATCGGGGCCTTTTTTACCCGGCCAGTCACGGACGTTCAGTTACCGGCT‑TGCCTGAATAGCAATCAAACCGGAGCCACATATGCGCGGGCAGATTGTTGACAAAGGGGGCTTTGTTCATGCCGGATACgg > 2:240277/1‑143 (MQ=255) cAAGGTTCAAATCTATCGGGGCCTTTTTTACCCGGCCAGTCACGGACGTTCAGTTACCGGCT‑TGCCTGAATAGCAATCAAACCGAAGCCACATATGCGCGGCCAGATTGTTGACAAAGGGCGCTTTGTTGATGCCGGATACgg < 2:382715/143‑1 (MQ=255) agcttCAAATCTATCGGGGCCTTTTTTACCCGGCCAGTCACGGACGTTCAGTTACCGGCT‑TGCCTGAATAGCAATCAAACCGAAGCCACATATGCGCGGCCAGATTGTTGACAAAGGGCGCTTTGTTCATGCCGTATCCGGCa < 1:676289/140‑1 (MQ=255) aGGTTCAAATCTATCGGGGCCTTTTTTACCCGGCCAGTCACGGACGTTCAGTTACCGGCT‑TGCCTGAATAGCAATCAAACCGAAGCCACATATGCGCGGCCAGATTGTTGACAAAGGGCGCTTTGTTCATGCCGGATACGGCa < 1:10762/143‑1 (MQ=255) aGGTTCAAATCTATCGGAGCCTTTTTTTCCCGTCCAGTCACGGACGTTCAGTTACCGGCT‑TGCCTGAATAGCAATCAAACCGAAGCCACATATGCGCGGCCAGATTGTTGACAAAGGGCGCTTTGTTCATGCCGGATACGGCa < 1:173354/143‑1 (MQ=255) gTTCAAATCTATCGGGGCCTTTTTTACCCGGCCAGTCACGGACGTTCAGTTACCGGCT‑TGCCTGAATAGCAATCAAACCGAAGCCACATATGCGCGGCCAGATTGTTGACAAAGGGCGCTTTGTTCATGCCGGATACGGCacg > 2:31284/1‑141 (MQ=255) ggttATCGGGGCCTTTTTTACCCGGCCGGTCACGGACGTTCAGTTACCGGCT‑TGCCGGAATAGCAATCAAACCCACGCCACATATGCGCGGCCAGATCGTTGACAAAGGGCGCTTCGTTCATGCCGTATCCGGCATGAAcaaa < 2:981399/140‑4 (MQ=255) ttgcGGGGCCTTTTCTGCCCGGGCAGTCGCGGACGGTCAGTTACCGGCT‑TGCCTGAACAGCGATCAAGCCGCTGCCACATATGCGCGGCCAGATTGTTGACCAAGGGCGCTTTGTTCATGCCGGATACGGCATGCCCGCtttt < 2:853344/140‑2 (MQ=255) gcgCCTTTTTTACCCGGCCAGTCACCGACGTTCAGTTACCGGCT‑TGCCTGAATATCAATCAAACCTAAGCCACATATGCTCTGCCATATTGTTGACAAAGGGCTCTTTGTTCATGCCGGATACGGCATGAACGCTTTATTCgg < 1:569090/141‑1 (MQ=255) gggcTTGTTTACCCGGCCGGTCACGGACGTTCAGTTACCGGCT‑TGCCGGAATAGCAATCAAACCGACGCCACATGTGCGCGGCCAGATTCTTGACAAAGGGCGCTTTGTTCATGCCGGATACGGCATGAACGCTTTATTCGGt < 2:888806/140‑1 (MQ=255) tttCCCCGGCCAGTCACGGACGTTCAGTTACCGGCT‑TGCCTGAATAGCAATCAAACCGCCGCCACATATGCGCGGCCAGATTGTTGACAAAGGGCGCTTTGTTCATGCCGGATACGGCATGAACGCTTTATTCGGTCTACaaa < 1:310125/143‑1 (MQ=255) ttACCCGGCTATTCACGGACGTTCAGTTACCGGCT‑TGCCTGAATAGCAATCAAACCGAAGCCACATATGCGCGGCCAGATTGTTGACAAAGGGCGCTTTGTTCATGCCGGATACGGCATGAACGCTTTATTCGGTCTACaaaa < 1:553329/143‑1 (MQ=255) cccGGCCAGTCACGGACGTTCAGTTACCGGCT‑TGCCTGAATAGCAATCAAACCGACGCCACATATGCGCGGCCAGATTGTTGACAAAGGGCGCTTTGTTCATGCCGGATACGGCATGAACGCTTTATTCGGTCTACAAAAGCa < 2:621434/143‑1 (MQ=255) ccGGCCCGTCACGGTCGTTTAGTTACCGGCT‑TGCCTGAATAGCAATCAAACCGAGGCCACATATGCGCGGCCAGATTGTTGACATAGGGCGCTTTGTTCATGCCGGATACGGCATGAACGCTTTATTCGGTCTACAAAAGCAg < 2:546468/143‑1 (MQ=38) ccGGCCAGTCACGGACGTTCAGTTACCGGCT‑TGCCTGAATAGCAATCAAACCGACGCCACATATGCGCGGCCAGATTGTTGACAAAGGGCGCTTTGTTCATGCCGGATACGGCATGAACGCTTTATTCGGTCTACAAAAGCAg < 1:252581/143‑1 (MQ=255) aCGGACGTTCAGTTACCGGCT‑TGCCTGAATAGCAATCAAACCGAAGCCACATATGCGCGGCCAGATTGTTGACAAAGGGCGCTTTGTTCATGCCGGATACGGCATGAACGCTTTATTCGGTCTACAAAAGCAGGCAAATTCaa < 1:674969/143‑1 (MQ=255) aCGGACGTTCAGTTACCGGCT‑TGCCTGAATAGCAATCAAACCGAAGCCACATATGCGCGGCCAGATTGTTGACAAAGGGCGCTTTGTTCATGCCGGATACGGCATGAACGCTTTATTCGGTCTACAAAAGCAGGCAAATTCaa < 2:929738/143‑1 (MQ=255) gTTCAGTTACCGGCT‑TGCCTGAATAGCAATCAAACCGAAGCCACATATGCGCGGCCAGAATGTTGACAAAGGGCGCTTTGTTCATGCCGGATACGGCATGAACGCATTATTCGGTCTACAAAAGCAGGCAAATTCAATATAtt > 2:20612/1‑143 (MQ=255) tCAGTTACCAGCTCTTCCT‑AATAGCGATCAAACCGAAGCCACATATGCGCGGCCAGATTGTTGACAAAGGGCGCGTTGTTCATGCCGGATACGGCATGAACGCTTTATTCGGTCTACAAAAGCAGGCAAATTCAATATATTGc < 1:1134183/143‑1 (MQ=38) ccgTTACCGGCT‑TGCCTGAACAGCAATCAACCCGAAGCCACATATGCGCGGCCAGATTGTGGACAAAGGGCGCTTTGTTCATGCCGGATACGGCATGAACGCTTTATTCGGTCTACAAAAGCAGGCAAATTCAATATATTGCa < 1:740560/141‑1 (MQ=255) cAGTTACCTGCT‑TGCCTGAATAGCAATCAAACCGAAGCCACATATGCGCGGCCAGATTGTTGACAAAGGGCGCTTTGTTCATGCCGGATACGGCATGAACGCTTTATTCGGTCTACAAAAGCAGGCAAATTCAATATATTGCa < 2:844684/143‑1 (MQ=255) cAGTTACCGGCT‑TGCCTGAATAGCAATCAAACCGAAGCCACATATGCGCGGCCAGATTGTTGACAAAGGGCGCTTTGTTCATGCCGGATACGGCATGAACGCTTTATTCGGTCTACAAAAGCAGGCAAATTCAATATATTGCa < 2:317931/143‑1 (MQ=255) gTTACCGGCT‑TGCCTGAATAGCAATCAAACCGAAGCCACATATGCGCGGCCAGATTGTTGACAAAGGGCGCTTTGTTCATGCCGGATACGGCATGAACGCTTTATTCGGTCTACAAAAGCAGGCAAATTCAATATATTGCaga < 1:368933/143‑1 (MQ=255) atcGGCT‑TGCCTGAATAGCAATCAAACCGAAGCCACATATGCGCGGGCCGATTGTTGACAAAGGGCGCGTGGTTCATGCCGGATACGGCATGAACGCTTTATTCGGTCTACAAAAGCAGGCAAATTCAATATATTGCAGAgat < 2:529646/141‑1 (MQ=255) cGGCT‑TGCCTGAATAGCAATCAAACCGAAGCCACATATGCGCGGCCAGATTGTTGACAAAGGGCGCTTTGTTCATGCCGGATACGGCATGAACGCTTTATTCGGTCTACAAAAGCTGGCAAATTCAAGAGATTGCAGAgatga > 2:838650/1‑143 (MQ=255) cT‑TGCCTGAATAGCAATCAAACCGAAGCCACATATGCGCGGCCAGATTGTTGACAAAGGGCGCTTTGTTCATGCCGGATACGGCATGAACGCTTTATTCGGTCTACAAAAGCAGGCAAATTCAATATATTGCAGAGATGATGt < 1:1103997/143‑1 (MQ=255) gCCTGAATAGCAATCAAACCGAAGCCACATATGCGCGGCCAGATTGTTGACAAAGGGCGCTTTGTTCATGCCGGATACGGCATGAACGCTTTATTCGGTCTACAAAAGCAGGCAAATTCAATATATTGCAGAGATGATGTAGa < 1:512286/143‑1 (MQ=255) cTGAATAGCAATCAAACCGAAGCCACATATGCGCGGCCAGATTGTTGACAAAGGGCGCTTTGTTCATGCCGTATCCGGCAt > 1:439107/1‑81 (MQ=255) cTGAATAGCAATCAAACCGAAGCCACATATGCGCGGCCAGATTGTTGACAAAGGGCGCTTTGTTCATGCCGTATCCGGCAt < 2:439107/81‑1 (MQ=255) gAATAGCAATCAAACCGAAGCCACATATGCGCGGCCAGATTGTTGACAAAGGGCGCTTTGTTCATGCCGGATACGGCATGAACGCTTTATTCGGTCTACAAAAGCAGGCAAATTCAATATATTGCAGAGATGATGTAGACACt > 2:885167/1‑143 (MQ=255) aTCAAACCGAAGCCACATATGCGCGGCCAGATTGTTGACAAAGGGCGCTTTGTTCATGCCGGATACGGCATGAACGCTTTATTCGGTCTACAAAAGCAGGCAAATCCAATATATTGCAGAGAGGATGTAGACACTGGCAAGCg > 1:1158381/1‑143 (MQ=255) tCAAACCGAAGCCACATATGCGCGGCCAGATTGTTGACAAAGGGCGCTTTGTTCATGCCGGCTACGGCATGAACGCTTTATTCGGCCTACAAAAGCAGGCAAACTCAATATATTGCAGAGCTGATGTAGACACTGGCAACCGt > 2:389187/1‑143 (MQ=38) aaCCGAAGCCACATATGCGCGGCCAGATTGTTGACAAAGGGCGCTTTGTTCATGCCGGATACGGCATGAACGCTTTATTCGGTCTACAAAAGCAGGCAAATTCAATTTATTGCAGAGATGATGTAGACACTGGCAAGCGTAgc < 1:824500/143‑1 (MQ=255) aaGCCACATATGCGCGGCCAGATTGTTGACAAAGGGCGCTTTGTTCATGCCGGATACGGCATGAACGCTTTATTCGGTCTACAAAAGCAGGCAAATTCAATATATTGCAGAgat > 2:1140439/1‑114 (MQ=255) aaGCCACATATGCGCGGCCAGATTGTTGACAAAGGGCGCTTTGTTCATGCCGGATACGGCATGAACGCTTTATTCGGTCTACAAAAGCAGGCAAATTCAATATATTGCAGAgat < 1:1140439/114‑1 (MQ=255) ccACATATGCGCGGCCAGATTGTTGACAAAGGGCGCTTTGTTCATGCCGGATACGGCATGAACGCTTTATTCGGTCTACAAAAGCAGGCAAATTCAATATATTGCAGAGATGATGTAGACACTGGCAAGCGTAGCGCATCAgg > 1:978519/1‑143 (MQ=255) acaTATGCGCGGCCAGATTGTTGACAAAGGGCGCTTTGTTCATGCCGGATACGGCATGAACGCTTTATTCGGTCTACACAAGCAGGCACATTCAATCTATTGCAGCGATGATGTAGACACTGGCAACCCTACCGCCTCAGGTa > 2:426940/1‑143 (MQ=25) tGCGCGGCCAGATTGTTGACAAAGGGCGCTTTGTTCATGCCGGATACGGCATGAACGCTTTATTCGGTCTACAAAAGCAGGCAAATTCAATATATTGCAGAGATGATGTAGACACTGGCAAGCGTAGCGCATCAGGTAAtttt < 2:935789/143‑1 (MQ=255) gcgcGGCCAGATTGTTGACAAAGGGCGCTTTGTTCATGCCGGATACGGCATGAACGCTTTATTCGGTCTACAAAAGCAGGCAAATTCAATATATTGCAGAGATGATGTAGACACTGGCAAGCGTAGCGCATCAGGTAATTTTg > 2:944471/1‑143 (MQ=255) ttgttgACAAAGGGCGCTTTGTTCATGCCGGATACGGCATGAACGCTTTATTCGGTATGCAAAAACACGCAAATTCAATATATTGCAGAGATGATGTAGACACTGGCAAGCGTAGCGCATCAGGTAATTTTGCGTTTATCTTc < 1:933630/143‑1 (MQ=255) tgttgACAAAGGGCGCTTTGTTCATGCCGGATACGGCATGAACGCTTTATTCGGTCTACAAAAGCAGGCAAATTCAATATATTGCAGAGATGATGTAGACACTGGCAAGCGTAGCGCATCAGGTAATTTTGCGTTTATCTTCa < 1:1257974/143‑1 (MQ=255) tgttgACAAAGGGCGCTTTGTTCATGCCGGATACGGCATGAACGCTTTATTCGGTCTACAAAAGCAGGCAAATTCAATATATTGAAGAGAGGATGTAGACACTGGCAAGCGTAGGGCATGAGGTAATTTAGCGTTTATCTTCa > 2:631661/1‑143 (MQ=25) gACAAAGGGCGCTTTGTTCATGCCGGATACGGCATGAACGCTTTATTCGGTCTACAAAAGCAGGCACATTCAATATATTGCAGAGATGATGTAGACACTGGCAAGCGTAGCGCATCAGGTAATTTTGCGTTTCTCTTCActct > 2:502817/1‑143 (MQ=255) gcAAAGGGCGCTTTGTTCATGCCGGATACGGCATGAACGCTTTAGTCGGTCTACAAAAGCAGGCAAATTCAATATATTGCAGAGATGATGTAGACACTGGCAAGCGTAGCGCATCAGGTAATTTTGCGTTTATCTTCACtctc < 2:108895/142‑1 (MQ=255) aCAAAGGGCGCTTTGTTCATGCCGGATACGGCATGAACGCTTTATTCGGTCTACAAAAGCAGGCAAATTCACTATATTGCAGCGCTCATGGAGACACTGGCAAGCGTAGCGCATCACGCAATTTTGCGTTTATCTTCACtctc > 2:147226/1‑143 (MQ=255) aCAAAGGGCGCTTTGTTCATGCCGGATACGGCATGAACGCTTTATTCGGTCTACAAAAGCAGGCAAATTCAATATATTGCAGAGATGATGTAGACACTGGCAAGCGTAGCGCATCAGGTAATTTTGCGTTTATCTTCACtctc > 2:1010841/1‑143 (MQ=255) aaaGGGCGCTTTGTTCATGCCGGATACGGCATGAACGCTTTATTCGGTCTACAAAAGCAGGCAAATTCAATATATTGCAGAGATGATGTAGACACTGGCAAGCGTAGCGCATCAGGTAATTTTGCGTTTATCTTCACTCTCaa < 1:178851/143‑1 (MQ=255) gcgcTTTGTTCATGCCGGATACGGGATGAACGCTTTATTCGGTCTACAAAAGCAGGCAAATTCAATATATTGCAGAGATGATGTAGACACTGGCAAGCGTAGCGCATCAGGTAATTTTGCGTTTATCTTCACTCTCAAGCCAc < 2:1062999/143‑1 (MQ=255) ttCATGCCGTATCCGGCATGAACGCTTTATTCGGTCTACAAAAGCAGGCAAATTCAATATATTGCAGAGATGATGTAGACACTGGCAAGCGTTGCGCATCACGTGATTTTGGGTTTATCTTCACTCTCAAGCCACGTTTAtgt > 1:650517/1‑143 (MQ=37) ttCATGCCGTATCCGGCATGAACGCTTTATTCGGTCTACAAAAGCAGGCAAATTCAATATATTGCAGAGATGATGTAGACACTGGCAAGCGTAGCGCATCAGGTAATTTTGCGTTTATCTTCACTCTCAAGCCACGTATAtgt > 1:810048/1‑143 (MQ=255) gCCGGATACGGCATGAACGCTTTATTCGGTCTACAAAAGCAGGCAAATTCAATATATTGCAGAGATGATGTAGACACTGGCAAGCGTAGCGCATCAGGTAATTTTGCGTTTATCTTCACTCTCAAGCCACGTATATGTGGCtt > 2:34356/1‑143 (MQ=255) ccGGATACGGCATGAACGCTTTATTCGGTCTACAAAAGCAGGCAAATTCAATATATTGCAGAGATGATGTAGACACTGGCAAGCGTAGCGCATCAGGTAATTTTGCGTTTATCTTCACTCTCAAGCCACGTATATGTGGCttt > 1:260309/1‑143 (MQ=255) ggATACGGCATGAACGCTTTATTCGGTCTACAAAAGCAGGCAAATTCAATATATTGCAGAGATGATGTAGACACTGGCAAGCGTAGCGCATCAGGTAATTTTGCGTTTATTTTCACTCTCAAGCCACGTATATGTGGCTTTAt > 1:498284/1‑143 (MQ=255) | GAAAATCAAGGTTCAAATCTATCGGGGCCTTTTTTACCCGGCCAGTCACGGACGTTCAGTTACCGGCT‑TGCCTGAATAGCAATCAAACCGAAGCCACATATGCGCGGCCAGATTGTTGACAAAGGGCGCTTTGTTCATGCCGGATACGGCATGAACGCTTTATTCGGTCTACAAAAGCAGGCAAATTCAATATATTGCAGAGATGATGTAGACACTGGCAAGCGTAGCGCATCAGGTAATTTTGCGTTTATCTTCACTCTCAAGCCACGTATATGTGGCTTTAT > NC_000913/770537‑770820 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |