Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	1,196,232	C→T	100%	T370T (ACC→ACT)	icd →	isocitrate dehydrogenase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,196,232	0	C	T	100.0%	79.3 / NA	31	T370T (ACC→ACT)	icd	isocitrate dehydrogenase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (13/18);  total (13/18)
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

CTGGTGCAAACATCGGTGACGAATGCGCCCTGTTTGAAGCCACCCACGGTACTGCGCCGAAATATGCCGGTCAGGACAAAGTAAATCCTGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCCACATGGGTTGGACCGAAGCGGCTGACTTAATTGTTAAAGGTATGGAAGGCGCAATCAACGCGAAAACCGTAACCTATGACTTCGAGCGTCTGATGGA  >  NC_000913/1196093‑1196315                                                                                                                                            |                                                                                    cTGGTGCAAACATCGGTGACGAATGCGCCCTGTTTGAAGCCACCCACGGTACTGCGCCGAAATATGCCGGTCAGGACAAAGTAAATCCTGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCCATATGGGTTGGACTGaa                                                                                  <  1:849210/143‑1 (MQ=255)  tGGTGCAAACATCGGTGACGAATGCGCCCTGTTTGAAGCCACCCACGGTACTGCGCCGAAATATGCCGGTCAGGACAAAGTAAATCCTGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCCATATGGGTTGGACTGAAg                                                                                 >  1:79483/1‑143 (MQ=255)   ggTGCAAACATCGGTGACGAATGCGCCCTGTTTGAAGCCACCCACGGTACTGCGCCGAAATATGCCGGTCAGGACAAAGTAAATCCTGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCCATATGGGTTGGACTGAAGc                                                                                <  2:121338/143‑1 (MQ=255)   ggTGCAAACATCGGTGACGAATGCGCCCTGTTTGAAGCCACCCACGGTACTGCGCCGAAATATGCCGGTCAGGACAAAGTAAATCCTGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCCATATGGGTTGGACTGAAGc                                                                                <  1:626514/143‑1 (MQ=255)     tGCAAACAGCGGTGACGAAGGCGCCCTGTTTGAAGCCACCCACGGTACTGCGCCGAAATATGTCGGTCAGGACAAAGTAAATCCTGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCCATATGGGTTGGACTGAAGCgg                                                                              <  1:643818/143‑1 (MQ=255)        aaaCATCGGTGACGAATGCGCCCTGTTTGAAGCCACCCACGGTACTGCGCCGAAATATGCCGGTCAGGACAAAGTAAATCCTGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCCATATGGGGTGGACTGAAGAGGCTg                                                                           >  1:946183/1‑143 (MQ=255)                     gAATGCTGCCTGTTTGAAGCCACCCACGGTTCTGCGCCGAAATATGCCCGTCAGGACAAAGTAGATCCTGGCTCTACTATTCTCTCCGCTGAGATGATGCTGCGCCATATGGGTTGGACTGAAGCGGCTGACCTGATTGTTaa                                                              <  2:1263698/143‑1 (MQ=37)                         gcgcCCGGTTTGACGCCACCCACTGTACTGCGCCGAAATATGCCGGTCAGGACAAAGTAAATCCTGGCTCTATTATTCTCCCCGCTGAGATGATGCTGCGCCATATGGGTTGGACTGAAGCGGCTGACCTGATTGTTAAAGGt                                                          <  2:965503/143‑1 (MQ=25)                           gcCCTGTTTGAAGCCACCCACGGTACTGCGCCTAAATATGCCGGTCAGGACCAAGTAAATCCTGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCCATATGGGTTGGACTGAAGCGGCTGACCTGATTGCTAAAgggag                                                        >  2:672316/1‑140 (MQ=37)                           gcCCTGTTTGAAGCCACCCACGGTACTGCGCCGAAATATGCCGGTCAGGACAAAGTAAATCCTGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCCATGTGGGTTGGACTGAAGCGGCTGACCTGGTTGTTACAGGTAt                                                        >  1:77194/1‑143 (MQ=38)                           gcCCTGTTTGAAGCCACCCACGGTACTGCGCCGAAATATGCCGGTCAGGACAAAGTAAATCCTGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCCATATGGGTTGGACTGCAGCGGCTGACCTGATTGTTCAAGGtct                                                        >  1:755463/1‑141 (MQ=38)                           gcCCTGTTTGAAGCCACCCACGGTACTGCGCCGAAATATGCCGGTCAGGACAAAGTAAATCCTGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCCATATGGGTTGGACTGAAGCGGCTGACCTGATTGTTAAAGGTAt                                                        >  1:56181/1‑143 (MQ=255)                           gcCCTGTTTCGAGCCACCCACGGTACTGCGCCGAAATATGCCGGTCAGGACAAAGTAAATCCTGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCCATATGGGTTGGACTGAAGCGGCTGACCTGATTGTTAAAGGTAt                                                        <  2:857229/143‑1 (MQ=37)                                gTTTGAAGCCACCCACGGTACTGCGCCGAAATATGCCGGTCAGGACAAAGTAAATCCTGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCCATATGGGTTGGACTGAAGCGGCTGACCTGATTGTTAAAGGTATGGAAg                                                   <  1:1068633/143‑1 (MQ=37)                                  ttGAAGCCACCCACGGTACTGCGCCGAAATATGCCGGTCAGGACAAAGTAAATCCTGGCTCTCTTATTCTCTCCGCTGAGATGATGCTGCGCCATATGGGTTGGACTGCAGCGGCTGCCCTGATTGTTAAAGGTATGGAAGgc                                                 >  1:895999/1‑143 (MQ=37)                                   tGAACCCACCCACCGCACTGCGCCGAAATATGCCGGTCAGGACAAACTAATTCCTGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCCTTATGGGTTGGACTGAAGCGGCTGACCTGATTGTTAAAGGTATGGAAGgcg                                                <  2:639161/143‑1 (MQ=21)                                    gAAGCCACCCACGCTACTGCGCCGAAATATGCCGGTCAGGACAAAGTAAATCCTGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCCATATGGGTTGGACTGAAGCGGCTGACCTGATTGTTAAAGGTATGGAAGgcgc                                               <  1:126869/143‑1 (MQ=25)                                           cccacGGTACTGCGCCGAAATATGCCGGTCAGGACAAAGTAAATCCTGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCCATATGGGTTGGACTGAAGCGGCTGACCTGATTGTTAAAGGTATGGAAGGCGCAATCaat                                        <  1:818577/143‑2 (MQ=25)                                           cccacGGTACTGCGCCGAAATATGCCGGTCAGGACAAAGTAAATCCTGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCCATATGGGTTGGACTGAAGCGGCTGACCTGATTGTTAAAGGTATGGAAGGCGCAATCaat                                        <  1:638526/143‑2 (MQ=25)                                                        tccGAAAGATGACGTTCAGGACAAAGTAATTCGTGGCTCTATTTGTCTCTCCGTTGAGATTATGCTGCGCCATATGTGTTGGACTGAAGCGGCTGACCTCATCTTTAAAGGTATGGAAGGCGCAATCAATGCCAAGACCGTaa                           <  2:106288/142‑1 (MQ=9)                                                        gctgAAATATGCGGGTCAGGACAATGTAAGTCCTGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCCATATGGGTTGGACTGAAGCGGCTGACCTGATTGTTAAAGGTATGGAAGGCGCAATCAATGCCAAGACCGTaa                           <  2:41365/140‑1 (MQ=17)                                                          cGAAATATGCCGGTTAGGACAAAGTAAATCCTGGCTCTATTATTCTCTCCGCTGAGATACTGCTGCGCCATCTGGGTTGGACTGAAGCGGCTGACCTGATTGTCAAAGGTATGGAAGGCGCAATCAATGACAAAACCGTaaat                         >  2:1149625/1‑141 (MQ=18)                                                            aaaTATGCCGGTCAGGACAAAGTAAATCCTGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCCATATGGGTTGGACTGAAGCGGCTGACCTGATTGTTAAAGGTATGGAAGGCGCAATCAATGCCAAGACCGTAACTTa                       >  2:1099251/1‑143 (MQ=17)                                                             atggtGCCGGTCAGGACAAAGTAAATCCTGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCCATATGGGTTGGACTGAAGCGGCTGACCTGATTGTTAAAGGTATGGAAGGCGCAATCAATGCCAAGACCGTAACTTAt                      <  1:337275/139‑1 (MQ=17)                                                             aaTATGCCGGTCAGGACAAAGTAAATCCTGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCTCCATATGGGTTGGACTGAAGCGGCTGACCTGATTGTTAAAGGTATGGAAGGCGCAATCAACGCCAAGACCGTAACTTAt                      <  1:799099/143‑1 (MQ=18)                                                               tatTCCGGTCAGGACAAAGTAATGCCTGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCCATATGGGGTGGACTGAAGCGGCTGACCTGATTGTTAAAGGTATGGAAGGCTCAATCAATGCCAAGACCGTAACTTATGa                    <  2:935412/143‑1 (MQ=14)                                                                  gCCGGTCAGGACAAATGAAACCCTGGCTTTATTATTCTCTCCGCTGAGATGATGCTGCGCCATATGGGTTGGACTGAAGCGGCTGACCTGATTGTTAAAGGTATGGAAGGCGCAATCAATGCCAAGACCGTAACTTATGACtt                 <  2:586518/143‑1 (MQ=14)                                                                           gACAAAGTAAATCCTGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCCATATGGGTTGGACTGAAGCGGCTGACCTGATTGTTAAAGGTATGGAAGGCGCAATCAATGCCAAGACCGTAACTTATGACTTCGAACGTCt        >  1:680353/1‑143 (MQ=11)                                                                              aaaGTAAATCCTGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCCATATGGGTTGGACTGAAGCGGCTGACCTGATTGTTAAAGGTATGGAAGGCGCAATCAATGCCAAGACCGTAACTTATGCCCTCGACCATCTcat     >  2:131160/1‑143 (MQ=14)                                                                                 gTAAATCCTGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCCATATGGGTTGGACTGAAGCGGCTGACCTGATTGTTAAAGGTATGGAAGGCGCAATCAATGCCAAGACCGTAACTTATGACTTCGAACGTCTgatgga  >  1:89575/1‑143 (MQ=11)                                                                                 gTAAATCCTGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCCATATGGGTTGGACTGAAGCGGCTGACCTGATTGTTAAAGGTATGGAAGGCGCAATCAATGCCAAGACCGAACCTTATGACTTCGAACGTCTgatgga  >  2:967793/1‑143 (MQ=11)                                                                                                                                            |                                                                                    CTGGTGCAAACATCGGTGACGAATGCGCCCTGTTTGAAGCCACCCACGGTACTGCGCCGAAATATGCCGGTCAGGACAAAGTAAATCCTGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCCACATGGGTTGGACCGAAGCGGCTGACTTAATTGTTAAAGGTATGGAAGGCGCAATCAACGCGAAAACCGTAACCTATGACTTCGAGCGTCTGATGGA  >  NC_000913/1196093‑1196315

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑