Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	1,992,083	G→C	6.3%	noncoding (35/76 nt)	glyW ←	tRNA‑Gly

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,992,083	0	G	C	6.3%	161.5 / 2.9	64	noncoding (35/76 nt)	glyW	tRNA‑Gly
Reads supporting (aligned to +/- strand):  ref base G (34/25);  new base C (2/2);  total (36/28)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.34e-01

AGACGGATTTGCAATCCGCTACATAACCGCTTTGTTAACGCGCCAAATTCTTCAGGCCTTTCAGCCAGACATCCGCTTGACGCCGATGTCTTTTAAACTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCATTCATCAAGCAATCAGTTAATCACTTGATTTTATTATCGTCTGGCAATCAGTGCCGCCGTTCGATGCGTTGCATTCTACTTACCTGGCGCGATGAGTCAACGA  >  NC_000913/1991944‑1992221                                                                                                                                            |                                                                                                                                           tgccgtctttGCAATCCGCTACATAACCTCTTTGTTAACGCGCCCAATTCTTCAGGCCTTTCAGCCAGACATCCGCTTGACGCCGATGTCTTTTAATCTGGAGCGCGAACCGAGACTCGAACTCGCGACCCCGACCTTGGCaa                                                                                                                                         <  2:387515/136‑1 (MQ=38)        tttGCAATCCGCTACACAACCGCTTTGTTAACGCGCCAAACTCTTCAGGCCTTTCAGCCAGACATCCGCTCGACGCCGATGTCTTTTAAACTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGCCAAGGTCggg                                                                                                                                  <  1:573691/143‑3 (MQ=38)         ttGCAATCCGCTACATAACCGCTTTGTTAACGCGCCAAATTCTTCAGGCCTTTCAGCCAGACATCCGCTTGACGCCGATGTCTTTTAAACTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGc                                                                                                                                 >  1:701828/1‑143 (MQ=255)         ttGCAATCCGCTACATAACCGCTTTGTTAACGCGCCAAATTCTTCAGGCCTTTCAGCCAGACACCCGCTTGACGCCGATGTCTTTTAAACTGGAGCGGGCAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGc                                                                                                                                 >  2:926346/1‑143 (MQ=255)          tGCAATCCGCTACATAACCGCTTTGTTAACGCGCCAAATTCTTCAGGCCTTTCAGCCAGACATCCGCTTGACGCCGATGTCTTTTCAACTGGAGCGTGAAACGAGACTCGAACTCGCTACCCCGACCTTGGCAAGGTCGTGct                                                                                                                                >  2:539863/1‑143 (MQ=255)          tGCAATCCGCTACATAACCGCTTTGTTAACGCGCCAAATTCTTCAGAGCATTCATCCAGACATCCGCTTGACGCCGATGTCTTTTAAACTGGAGCGGGAAGCGAGACTCGCACTCGCGACCCCGACCTTGGCCCGGTCGTGcc                                                                                                                                >  2:911833/1‑142 (MQ=38)           gCAATCCGCTACATAACCGCTTTGTTAACGCGCCAAATTCTTCAGGCCTTTCAGCCAGACATCCGCTTGACGCCGATGTCTTTTAAACTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGctc                                                                                                                               >  1:1274088/1‑143 (MQ=255)             aaTCCGCTACATAACCGCTTTGTTAACGCGCCAAATTCTTCAGGCCTTTCCGCCAGACATGCGCTTGACGCCGATGTCTTTTAAACTGGAGCTGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCCAGGTCGTTCTCTa                                                                                                                             >  1:656359/1‑143 (MQ=38)             aaTCCGCTACATAACCGCTTTGTTAACACGCCAAAGTCTTCAGGCCTTTCAGCCAGACATCCGCTTGACGCCGATGTCTTTTAAACTGGAGCGGGAAACGAGACGCGAACTCGCGACTCCGACCTTGGCAAGGTCGTGCTCTa                                                                                                                             >  1:1014140/1‑143 (MQ=255)               tCCGCTACATAACCGCTTTGTTAACGCGCCAAATTCTTCAGGCCTTTCAGCCAGACATCCGCTTGACGCCGATGTCTTTTAAACTGGAGCGGGAACCGAGACTCGAACTCGCGACCCCGGCCTTGGCAAGGTCGAGCTCTAcc                                                                                                                           >  2:1281310/1‑143 (MQ=255)                 cGCTACATAACCGCTTTGTTAACGCGCCAAATTCTTCAGGCCTTTCAGCCAGACATCCGCTTGACGCCGATGTCTTTTAAACTGGAGCGGGAAGCGAGCCGCGGACTCGCGACCCCGACCGTGGCATGGTAGTGGTGTAcaga                                                                                                                         >  1:124850/1‑140 (MQ=255)                 cGCTACATAACCGCTTTGTTAACGCGCCAAATTCTTCAGGCCTTTCAGCCAGACATCCGCTTGACGCCGATGTCTTTTAAACTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCaa                                                                                                                         <  2:1174804/143‑1 (MQ=255)                 cGCTACATAACCGCTTTGTTAACGCGCCAAATTCTGCAGGCCTTTCAGCCAGACATCCGCTTGACGCCGATGTCTTTTAAACTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGCGGGAGCTCTAccca                                                                                                                         >  2:393642/1‑141 (MQ=38)                    tACATAACCGCTTTGTTAACGCGCCAAATTCTTCAGGCCTTTCAGCCAGACATCCGCTTGACGCCGATGTCTTTTAAACTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTg                                                                                                                      <  1:775958/143‑1 (MQ=255)                    gaccgagccGCTTTGTTAACGCGCCAAATTCGTCAGGCCGTGCAGCCAGACATCCGCTTGACGCCGATGTCTTTTAAACTGGAGCGGGAATCGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTg                                                                                                                      <  1:763096/136‑1 (MQ=25)                     aCATAACCGCTTTGTTAACGCGCCAAGTTCTTCAGGCCTTTCAGCCAGACATCCGCGTGACGCCGATGTCTTTTAAACTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGa                                                                                                                     <  2:927511/143‑1 (MQ=255)                       aTAACCGCTTTGTTAACGCGCCAAATTCTTCAGGCCTTTCAGCCAGACATCCGCTTGACGCCGATGTCTTTTAAACTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCACGGTCGTGCTCTACCACCTGAGc                                                                                                                   >  1:91743/1‑143 (MQ=255)                            cGCTTTGTTACCGCGCCAAATTCTTCGGGCCTTTCAGCCAGACTTCCGCTTGACGCCGACGTCTTTTAAAGTGGAGCGGGAAACGAGACTCGAACTCGCGTCCGTGACCTTTTCAAGGTCGTGCTCTACCAACTGAGCTATTc                                                                                                              <  2:628519/143‑1 (MQ=25)                            cGCTTTGTTAACGCGCCGAATTCTTCAGGCCTTTCAGCCAGACACCCGCTTGACGCCGATGTCTTTTAAACTGCAGCGGGAAACGAGACTCGAACTCGCGATCCCGACCTTCCCAAAGCCGGGCTCTACCAACTGAGCTATTc                                                                                                              <  2:1119689/143‑1 (MQ=25)                            cGCTTTGTTAACGCGCCAAATTCTTCAGGCCTTTCAGCCAGACATCCGCTTGACGCCGATGTCTTTTAAACTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTc                                                                                                              >  2:924423/1‑143 (MQ=255)                            cGCTGTGTTAACGCGCCAAAGTCTCCGGGCCGTTCCGCCCGACATCCGCTTGACGCCGATGTCTTTTAAACTGGAGCCGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTc                                                                                                              <  2:6952/143‑1 (MQ=21)                              cTTTGTTAACGCGCCAAATTCTTCAGTCCTTTCAGCCAGACATCCGCTTGACGCCGATGTCTTTTAAACTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTccc                                                                                                            <  1:1049236/143‑1 (MQ=255)                              cTTTGTTAACGCGCCAAATTCTTCAGGCCTTTCAGCCAGACATCCGCTTGACGCCGATGTCTTTTAAACTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTccc                                                                                                            >  1:614662/1‑143 (MQ=255)                                ttGTTAACGCGCCAAATTCTTCAGGCCTTTCAGCCAGACATCCGCTTGACGCCGATGTCTTTTAATCTGGAGCGGGCAACGAGACTCGAACTCGCGACCCCGCCCTTGGCACGGTCGTGCTCTACCAACGCAGCCACTccccc                                                                                                          >  2:212978/1‑141 (MQ=25)                                ttGTTAACGCGCCAAATTCTTCAGGCCTTTCAGCCAGACATCCGCTTGACGCCGATGTCTTTTAAACTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGc                                                                                                          >  1:618118/1‑143 (MQ=255)                                    gaaCGCCCCAACTTCTTCAGGCCTTTCAGCCAGACATCCGCTGGACGCCGATGTCTTTTAAACTGGAGCGGGAAACGAGCCTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACTAACTGAGCTTTTCCCGCATtc                                                                                                      <  2:915005/142‑1 (MQ=25)                                      aCGCGCCAAATTCTTCAGGCCTTTCAGCCAGACATCCGCTTGACGCCGATGTCTTTTAAACTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCATtcat                                                                                                    >  2:559118/1‑143 (MQ=255)                                      aCGCGCCAAATTCTTCAGGCCTTTCAGCCAGACATCCGATTGACGCCGATGTCTTTTAAACTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCATtcat                                                                                                    <  1:912845/143‑1 (MQ=255)                                          gcCAAATTCTTCAGGCCTTTCAGCCAGACATCCGCTTGACGCCGATGTCTTTTAAACTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCATTCATCAAg                                                                                                <  1:223132/143‑1 (MQ=255)                                                   ttcAGGCCTTTCAGCCAGACATCCGCTTGACGCCGATGTCTTTTAAACTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCATTCATCAAGCAATCAGtt                                                                                       >  1:1156958/1‑143 (MQ=255)                                                    tcAGGCCTTTCAGCCAGACATCCGCTTGACGCCGATGTCTTTTAAACTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCATTCATCAAGCAATCAGTTa                                                                                      >  1:276032/1‑143 (MQ=255)                                                     cAGGCCTTTCAGCCAGACATCCGCTTGACGCCGATGTATTTTAAACTGGAGCGGGAAACGAGACTCGGACTCGCGACCCCGACCTTGGCGAGGTGGTGCCATACCAACTGAGCTATGCCCGCATTCCTCAAGCGATcccgtag                                                                                     >  2:684283/1‑137 (MQ=21)                                                     cAGGCCTTGCAGCCAGACATCGGCTGGACGCCGATGTCTTTGAAGGTGGTGCGGGGGGCGGGGGTGGAAGGCGCGGCCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCATGCATCAAGCAATCAGTTaa                                                                                     <  1:216508/143‑1 (MQ=18)                                                      aGGCCTGTCAGCCAGACATCCGCTTGACGCCGATGTCTTTTAAACTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCATTCATCAAGCAATCAGTTAAt                                                                                    <  1:130241/143‑1 (MQ=255)                                                                    gccATCCGCGTGACGCCGATGTCTTTTAAACTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCATTCATCAAGCAATCAGTTAATCACTTGATTttatt                                                                      <  2:417604/141‑1 (MQ=255)                                                                    gACATCCGCTTGACGCCGATGTCTTTTAAACTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCATTCATCAAGCAATCAGTTAATCACTTGATTttatt                                                                      <  1:1092507/143‑1 (MQ=255)                                                                          cgcgTGACGCCGATGTCTTTTAAACTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCATTCATCAAGCAATCAGTTAATCACTTGATTTTATTATCGTc                                                                <  2:640574/143‑1 (MQ=255)                                                                                  gCCGATGTCTTTTAAACTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCATTCATCAAGCAATCAGTTAATCACTTGATTTTATTATCGTCTGGCAATc                                                        >  2:104857/1‑143 (MQ=255)                                                                                        gTCTTTTAAACTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCATTCATCAAGCAATCAGTTAATCACTTGATTTTATTATCGTCTGGCAATCAGTgcc                                                  <  1:339512/143‑1 (MQ=255)                                                                                           ttttAAACTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCATTCATCAAGCACGCAGTTAGGCAGTTGATTTTATTATCGTCTGGCAACCAGTgccgcc                                               >  1:977862/1‑143 (MQ=21)                                                                                           ttatAAACTGGAGCGGGAAACGAGACTCGAACTCGCGAACCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCCTTCATCAAGCAATCCGCTAATCACTTGCTATTATTAGCGTCTGCCAAGCAGTgcgccc                                               >  2:433924/4‑139 (MQ=18)                                                                                                aaCTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCATTCATCAAGCAATCAGTTAATCACTTGATTTTATTATCGTCTGGCAATCAGTGCCGCCGTTCg                                          >  2:997261/1‑143 (MQ=255)                                                                                                 aCTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCATTCATCAAGCAATCAGTTAATCACTTGATTTTATTATCGTCTGGCAATCAGTGCCGCCGTTCGa                                         <  1:559118/143‑1 (MQ=255)                                                                                                  cTGGCGCGGGAGACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGCGCTCTACCAACTGAGCTATTCCCGCATTCATCAAGCAATCAGTTAATCACTTGATTTTATTATCGTCTGGCAATCAGTGCCGCCGTTCGAt                                        <  1:401035/143‑1 (MQ=255)                                                                                                   tGGACCGGGAAACGAGACTCGACCTCGCGCCCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCATTCATCAAGCAATCAGTTAATCACTTGATTCTATTATCGTCTGGCAAGCAGTGCCGCCGTTCGATg                                       <  1:834468/143‑1 (MQ=25)                                                                                                     gAGCGGGAAACGAGACTCGAACGCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCATTCATCAAGCAATCAGTTAATCACTTGATTTTATTATCGTCTGGCAATCAGTGCCGCCGTTCGATGCg                                     <  2:1128460/143‑1 (MQ=255)                                                                                                        cGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCATTCATCAAGCAATCAGTTAATCACTTGATTGTATTATAGTCTGGCAATCAGTGCCGCCGTTCGATGCGTTg                                  >  2:766695/1‑143 (MQ=255)                                                                                                          ggAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCATTCATCAAGCAATCAGTTAATCACTCGATTTTATTATCGTCTGGCAATCAGTGCCGCCGTTCGATGCGTTGCa                                >  1:481650/1‑143 (MQ=255)                                                                                                           gAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAAATGAGCTATTCCCGCATTCATCAAGCAATCAGCTAAACACTTGATCTTATTATCGCCTGGCAATCAGTACCGCCGATCGACGCGtgaccc                               >  2:313014/1‑138 (MQ=21)                                                                                                             aaCGAGACTCGAACTCGCGCACCCGTGCGTTGCAATTGCGTGCTCTACCAACTGCGCTATCCCCGCATTCATCAAGCAATCAGTTAATCACCTGATATTATTATCGCCTGGCCATCAGTGCCGCCGCTAGATGCGTTGCCTTc                             >  2:457301/1‑143 (MQ=255)                                                                                                                gagaCTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTACCGCATTCATCAAGCAATCAGTTAATCACTTGATTTTATTATCGTCTGGCAATCAGTGCCGCCGTTCGATGCGGTGCATTCTAc                          >  2:154372/1‑143 (MQ=255)                                                                                                                     tCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCATTCATCAAGCAATCAGTTCATCACTTGATTTTATTATCATCTGGCAATCAGTGCCGCCGTTCGATACGTTTCATTCTCCTTAcg                     >  2:528123/1‑142 (MQ=37)                                                                                                                      cgcgcTCGCGTCCCCGACCTTGGCAAGGTCGAGCTCTACCAACTGAGCTATTCCCGCATTCATCAAGCAATCAGTTAATCACTTGATTTTATTATCGTCTGGCAATCAGTGCCGCCGTTCGATGCGTTGCATTCTACTTACCt                    <  2:804077/139‑1 (MQ=255)                                                                                                                       gAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCATTCATCAAGCAATCAGTTAATCACTTGATTTTATTATCGTCTGGCAATCAGTGACGCCGTTCGATGCGTTGCATTCTACTAACCTg                   >  2:1274627/1‑143 (MQ=255)                                                                                                                           tCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCATTCATCAAGCAATCAGTTAATCACTTGATTTTATTATCGTCTGGCAATCAGTGCCGCCGTTCGATGCGTTGCATTCTACTTACCTGgcgc               <  1:212978/143‑1 (MQ=255)                                                                                                                             gcgACCCCGACCTTGGCAAGGTCGTGCTCTACCCACTGAGCTATTCCCGCATTCATCAAGCAATCAAATAAACACTTGATTTTATTATCGTCTGGCAATCAGTGCCGCCGTTCGATGAGTTGCATTCTACTTACCTGGCGCGa             >  1:483561/1‑143 (MQ=38)                                                                                                                              cgACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCATTCATCAAGCAATCAGTTAATCACTTGATTTTATTATCGTCTGGCAATCAGTGCCGCCGTTCGATGCGTTGCATTCTACTTACCTGGCGCGAt            <  2:1243538/143‑1 (MQ=255)                                                                                                                              cgACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCATTCATCAAGCAATCAGTTAATCACTTGATTTTATTATCGTCTGGCAATCAGTGCCGCCGTTCGATGCGTTGCATTCTACTTACCTGGCGCGAt            <  2:1274088/143‑1 (MQ=255)                                                                                                                               gagcacGACCTTGCCAAGGTCGGGGTCTACCGACTGAGCTATTCCCGCATTCATCACGCAATCAGTTAATCACTTGATTTTATTCTCCTATGGCAATCAGTGCCACCGTCCGCTCCCCTGCATTCTACTCCCCTGGCGCGacg           >  2:1161451/6‑141 (MQ=255)                                                                                                                                gcgccGAGCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCATTCATCAAGCAATCAGTTAATCACTTGATTTTATTATCGTCTGGCAATCAGTGCCGCCGTTCGATGCGTTGCATTCTACTTACCTGGCGCGATGa          <  2:91743/140‑1 (MQ=255)                                                                                                                                aCCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCATTCATCAAGCAATCAGTTAATCACTTGATTTTATTATCGTCTGGCAATCAGTGCCGCCGTTCGATGCGTTGCATCATACTTACCTGGCGCGagac          >  2:1252645/1‑140 (MQ=255)                                                                                                                                   acGACCTTGCCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCATTCATCAAGCAATCAGTTAATCACTTGATTTTATTATCGTCTGGCAATCAGTGCCGCCGTTCGATTCGTTTCATTCTAGTTACATGGCGCGATGAggt       >  2:864672/2‑141 (MQ=38)                                                                                                                                       ccTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCATTCATCACGCAATCAGTTACTCACTTGATTTTATTATCGTCTGGCAATCAGTGCCGCCGTTCGATGCGTTGCATTCTACTTACCTGGCGCCATGAGTCAACg   >  2:917682/1‑143 (MQ=255)                                                                                                                                        cTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCATTCATCAAGCAATCAGTTAATCACTTGATTTTATTATCGTGTGGCAATCAGTGCCGCCGTTCGATGCGTTGCATTCTACTTACCTGGCGCGATGAGTCAACGa  >  1:641886/1‑143 (MQ=255)                                                                                                                                            |                                                                                                                                           AGACGGATTTGCAATCCGCTACATAACCGCTTTGTTAACGCGCCAAATTCTTCAGGCCTTTCAGCCAGACATCCGCTTGACGCCGATGTCTTTTAAACTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCATTCATCAAGCAATCAGTTAATCACTTGATTTTATTATCGTCTGGCAATCAGTGCCGCCGTTCGATGCGTTGCATTCTACTTACCTGGCGCGATGAGTCAACGA  >  NC_000913/1991944‑1992221

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑