Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,081,688 A→T 7.7% I287N (ATT→AAT)  dacD ← D‑alanyl‑D‑alanine carboxypeptidase DacD

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,081,6880AT 7.7% 160.0 / 4.3 64I287N (ATT→AAT) dacDD‑alanyl‑D‑alanine carboxypeptidase DacD
Reads supporting (aligned to +/- strand):  ref base A (33/24);  new base T (3/2);  total (37/27)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.02e-01

ATCAAGGGTATATTTGGCTTTGATATGTGGAATTTCGGCTTTCGGTAGCACCATCCAGAACTCTTGTTCCGTTCCCAGGTCGATATTTTCTTTATCGCCATACCAGATGCGTTCCGTTCCGACCTTTTTCCCACGGTGCAAAATTTGCACC‑GTAGTAAAGTTTTGTTGCCCCCAACGCAGTAATTTTCTTGCCTCTTCCTCACGACCTTTTGCACTGTCAGCCCCCATTACCACTGCAATGAGACGACGCTG‑CCCATCTACAGCCGAAGCAATGAGATTAAAC  >  NC_000913/2081546‑2081828
                                                                                                                                              |                                                                                                                                              
aTCGAGGGTATATTTGGCCTTGATATGTGGAATTTCGGCTTGCGGTAGCACCATCCAGAACTCTTGTTCCGTTCCCAGGTCGATATTTTCTTTATCGCCATACCAGATGCGTTCCGTTCCGACCTTTTTCCCACGGTGCaaaa                                                                                                                                                <  2:1077377/143‑1 (MQ=255)
   aaGGGTATATTTGGCTTTGATATGTGGAATTTCGGCTTTCGGTAGCACCATCCAGAACTCTTGTTCCGCTCCCGGGTCGGTATTTTCTTTATCGCCATACCAGATGCGTTCCGTTCCGACCTTTTTCCCCCGGTGCACAAttc                                                                                                                                             >  2:82831/1‑142 (MQ=255)
      ggTATATTTGGCTGTGGTAGGTGGAATTTCGGCTTTCGGTAGCACCATCCAGAACTCTTGTTCCGTTCCCAGGTCGATATTTTCTTTATCGCCATACCCGATGCGTTCCGTTCCGACCTTTTTCCCACGGTGCAAAATTTGCa                                                                                                                                          <  2:131022/143‑1 (MQ=255)
       gTATATTTGGCTTTGATATGTGGAATTTCGGCTTTCGGTAGCACCATCCAGAACTCTTGTTCCGTTCCCAGGTCGATATTTTCTTTATTGCCATACCAGATGCGTTCCGTTCCGACCTTTTTCCCACGGTGCAAAATTTGCAc                                                                                                                                         <  1:666111/143‑1 (MQ=255)
        tataTTTGGCTTTGATATGTGGAATTTCGGCTTTCGGTAGCACCATCCAGAACTCTTGTTCCGTTCCCAGGTCGATATTTTCTTTATCGCCATACCAGATGCGTTCCGTTCCGACCTTTTTCCCACGGTGCCAAATTTGcccc                                                                                                                                        >  1:911661/1‑143 (MQ=255)
         atatTTGGCTTTGATATGTGGAATTTCGGCTTTCGGTAGCACCATCCAGAACTCTTGTTCCGTTCCCAGGTCGATATTTTCTTTATCGCCATACCAGATGCGTTCCGTTCCGACCTTTTTCCCACGGTGCAAATTTTGCACC‑g                                                                                                                                      <  1:119315/143‑1 (MQ=255)
           atTTGGCTTTGATATGTGGAATGTCGGCTTTCGGTAGCACCATCCAGAACTCTTGTTCCGTTCCCAGGTCGATATTTTCTTTATCGCCATACCAGATGCGTTCCGTTCCGACCTTTTTCCCACGGTGCAAAATTTGCACC‑gta                                                                                                                                    <  1:29055/143‑1 (MQ=255)
            tttGGCTTTGATATGTGGAATTTCGGCTTTCGGTAGCACCATCCAGAACTCTTGTTCCGTTCCCAGGTCGATATTTTCTTTATCGCCATACCAGATGCGTTTCGTTGTCACCCTTCTCCCACGGTGCCAAATTTGCACC‑gtag                                                                                                                                   >  2:899898/1‑143 (MQ=255)
            tttGGCTTTGATATGTGGAATTTCGGCTTTCGGTAGCACCATCCAGAACTCTTGTTCCGTTCCCAGGTCGATATTTTCTTTATCGCCATACCAGATGCGTTCCGTTCCGACCTTTTTCCCACGGTGCAAAATTTGCAAC‑gtag                                                                                                                                   >  2:470827/1‑143 (MQ=255)
                  tttGATATGTGGAATTTCGGCTTTCGGTAGCACCATCCAGAACTCTTGTTACGTTCGCAGGGCGCTAATACAATTCACGCCATTCCAGATGCGTTCCGTTCCGCCCTTTTTCCCACGGTGCAAAATTTGCACC‑GTAGTAAAGt                                                                                                                             >  1:1275483/1‑143 (MQ=255)
                     gATATGTGGAATTTCGGCTTTCGGTAGCACCATCCAGAACTCTTGTTCCGTTCCCAGGTCGATATTTTCTTTATCGCCATACCAGATGCGTTCCGTTCCGACCTTTTTCCCACGGTCCCAAATTTGCCCC‑GTCGTCACGtttt                                                                                                                          >  1:1189399/1‑143 (MQ=255)
                      atatGTGGAATTTCGGCTTTCGGTAGCACCATCCAGAACTCTTGTTCCGTTCCCAGGTCGATATTTTCCTTATCGCCATACCAGATGCGTTCCGTTCCGACCTTTTTCCCACGGTGCAAAATTTGCACC‑GTAGTAAAGTTttg                                                                                                                         >  1:1041066/1‑143 (MQ=255)
                        atGTGGAATTTCGGCTTTCGGTAGCACCATCCAGAACTCTTGTTCCGTTCCCAGGTCGATATTTTCTTTATCGCCATACCAGATGCGTTCCGTTCCGACCTTTTTCCCACGGTGCAAAATTTGCACC‑GTAGTAAAGTTttgtt                                                                                                                       <  2:1099493/143‑1 (MQ=255)
                        atGTGGAATTTCGGCTTTCGGTAGCACCATCCAGAACTCTTGTTCCGTTCCCAGGTCGAGATTTTCATTTTCGCCATACCAGATGCGTTCCGTTCCGACCTTTTTCCCACGGTGCAAAATTTGCACA‑GTAGCAAAGCTttgtt                                                                                                                       >  2:622196/1‑143 (MQ=255)
                         tgtgGAATGTCGGCTTTCGGTAGCACCAGCCAGAACTCTTGTTCCGTTCCCAGGTCGATATTTTCTTTATCGCCATACCAGATGCGTTCCGTTCCGACCTTTTTCCCACGGTGCAAAATTTGCACC‑GTAGTAAAGTTttgttg                                                                                                                      <  1:1215034/143‑1 (MQ=255)
                            ggtctctCGGCTTTCGGGCGCACCATCCAGTCTTCTTGTTCCGTTCCCAGGTCGATCTTTTCTTTATCGCCATACCAGATGCGTTCCGTTCCGACCTTTTTCCCACGGTGCAAAATTTGCACC‑GTAGTAAAGTTTTGTTGccc                                                                                                                   <  2:649833/137‑1 (MQ=255)
                            ggAATTTCGGCTTTCGGTAGCACCATCCAGAACTCTTGTTCCGTTCCCAGGTCGATATTTTCTTTATCGCCATACCAGATGCGTTCCGTTCCGACCTTTTTCCCACGGTGCAACATTTGCACC‑CTAGTAAAGTTTTGTTGccc                                                                                                                   >  1:1201113/1‑143 (MQ=255)
                             gAATTTCGGCTTTCGGTAGCACCATCCAGAACTCTTGTTCCGTTCCCAGGTCGATATTTTCTTTATCGCCATACCAGATGCGTTCCGTTCCGACCTTTTTCCCACGGTGCACAATTTGCACC‑GTAGTAAAGTTTCGTTGcccc                                                                                                                  >  2:768443/1‑143 (MQ=255)
                                tttCGGCTTTCGGTAGCACCATCCAGAACTCTTGTTCCGTTCCCAGGTCGATATTTTCTTTATCGCCATACCAGATGCGTTCCGTTCCGACCTTTTTCCCACGGTGCAAAATTTGCACC‑GTAGTAAAGTTTTGTTGCCCCCaa                                                                                                               <  1:220184/143‑1 (MQ=255)
                                      cTTTCGGTAGTACCATTCAGAACTCTTGTTCCGTTCCCAGGTCGATATTTTCTTTATCGCCATACCAGATGCGTTCCGTTCCGACCTTTTTCCCACGGTGCAAAATTTGCACC‑GTAGTAAAGTTTTGTTGCCCCCAACGCAGt                                                                                                         <  2:390927/143‑1 (MQ=255)
                                      cTTTCGGTAGCACCATCCAGAACTCTTGTTCCGTTCCCAGGTCGATATTTTCTTTATCGCCATACCAGATGCGTTCCGTTCCGACCTTTTTCCCACAGCGCAAAATTTGCACC‑GTAGCAACGCTTTGTTGCCCCCCACGCaca                                                                                                         >  2:863327/1‑141 (MQ=255)
                                      cTTTCGGTAGCACCATCCAGAACTCTTGTTCCGTTCCCAGGTCGATATTGTCTTTATCGCCATACCAGATGCGTTCCGTTCCGACCTTTTTCCCACGGTGCAAAATTTGCACC‑GCAGTCAAGTTTTGTTGCCCCCAACGCAGt                                                                                                         >  2:1242696/1‑143 (MQ=255)
                                       tttCGGTAGCACCATCCAGAACTCTTGTTCCGTTCCCAGGTCGATATTTTATTTATCGCCATACCAGATGCGTTCCGTTCCGACCTTTTTCCCACGGTGCAAAATTTGCACC‑GTAGTAAAGTTTTGTTGCGCCCAACGCAGtc                                                                                                        >  1:682288/1‑142 (MQ=255)
                                         tCGGTAGCACCATCCAGAACTCTTGTTCCGTTCCCAGGTCGATATTTTCTTTATCGCCATACCAGATGCGTTCCGTTCCGACCTTTTTCCCACGGTGCAAAATTTGCACC‑GTAGTAAAGTTTTGTTGCCCCCAACGCAGTAAt                                                                                                      <  2:432485/143‑1 (MQ=255)
                                               gCACCATCCAGAACTCTTGTTCCGTTCCCAGGTCGATATTTTCTTTATCGCCATACCAGATGCGTTCCGTTCCGACCTTTTTCCCACGGTGCAAAATTTGCACC‑GTAGTAAAGTTTTGTTGCCCCCAACGCAGTAATTTTCtt                                                                                                <  2:1144033/143‑1 (MQ=255)
                                               gCACCATCCAGAACTCTTGGTCCGTTCCCAGGTCGATATTTTCTTTATCGCCATACCAGATGCGTTCCGTTCCGACCGTTTTCCCACGGTGCAAAATTTGCACC‑GTAGTAAAGTTTTGTTGCCCCCAACGCAGTAATTTTCtt                                                                                                <  2:1179090/143‑1 (MQ=255)
                                                     tCCAGAACTCTTGTTCCGTTCCCAGGTCGATATTTTCTTTATCGCCATACCAGATGCGTTCCGTTCCGACCTTTTTCCCACGGTGCAACATTTGCACC‑GTAGCCCAGCCCAGTAGCGCCCAGCGCAGTAATTTTCTTGCctct                                                                                          >  1:532187/1‑143 (MQ=255)
                                                     tCCAGAACTCTTGTTCCGTTCCCAGGTCGATATTTTCTTTATCGCCATACCAGATGCGTTCCGTTCCGACCTTTTTCCCACGGTGCAAAATTTGCACC‑GTAGTAAAGTTTTGTTGCCCCCAACGCAGTAATTTTCTTGCctct                                                                                          <  1:1047557/143‑1 (MQ=255)
                                                      ccAGAACTCTTGTTCCGTTCCCAGGTCGATATTTTCTTTATCGCCATACCAGATGCGTTCCGTTCCGACCTTTTTCCCACGGTGCAAAATTTGCACC‑GTAGTAAAGTTTTGTTGCCCCCAACGCAGTAATTTTCTTGCCTCtt                                                                                         >  1:967856/1‑143 (MQ=255)
                                                        aGAACTCTTGTTCCGTTCCCAGGTCGATATTTTCTTTATCGCCATACCAGATGCGTTCCGTTCCGACCTTTTTCCCACGGCCCAAAAATTGCACC‑GTAGTAAAGTTTTGTCGCCCCCAACTGAGTAACTTACTTGCCTCTTcc                                                                                       >  2:192328/1‑143 (MQ=255)
                                                          caCTCTTGTTCCGTTCCCCGGTCGATATTTTCTTTATCGCCATACCAGATGCGTTCCGTTCCGACCTTTTTCCCACGGTGCAAAATTTGCACC‑GTAGTAAAGTTTTGTTGCCCCCAACGCAGTAATTTTCTTGCCTCTTCCTc                                                                                     <  2:974234/142‑1 (MQ=255)
                                                              ctTGTTCCGTTCCCAGGTCGATATTTTCTTTATCGCCATACCAGATGCGTTCCGTTCCGACCTTTTTCCCCCGGTTCCAACTTTGCCCC‑GTAGTCAAGCTTTGTTGCCCCTCACGCCGTAGTTTTATTGCTTATTCCTAACGa                                                                                 >  1:380840/1‑143 (MQ=255)
                                                               ttGTTCCGTTCCCAGGTCGATATTTTCTTTATCGCCATACCAGATGCGTTCCGTTCCGACCTTTTTCCCACGGTGCAAAATTTGCACC‑GTAGTAAAGTTTTGTTGCCCCCACCGCAGTAATTTTCTTGCCTCTTCCTCACgcc                                                                                >  2:399999/1‑141 (MQ=255)
                                                                 gttccgttccCAGGTCGATATTTTCTTTATCGCCATACCAGATGCGTTCCGTTCCGACCTTTTTCCCACGGTGCAAAA‑TTGAACCAGAAAGGAGGGTTTGTTGCCCCCAACGCAGTAACTTTCTTGCCTCTTCCTCACGAcca                                                                              >  2:145236/1‑142 (MQ=255)
                                                                 gttccgttccCAGGTCGATATTTTCTTTATCGCCATACCAGATGCGTTCCGTTCCGACCTTTTTCACACGGTGCAAAATTTGCACC‑GTAGTAAAGCTTTGTTGCCCCCAAGGCAGTCATTTTCTTGCCTCTTCCTCACGACCt                                                                              >  2:348494/1‑143 (MQ=255)
                                                                     cgttccCAGGTCGATATTTTCTTTATCGCCATACCAGATGCGTTCCGTTCCGACCTTTTTCCCACGGTGCAAAATTTGCACC‑GTAGTAAAGTTTTGTTGCCCCCAACGCAGTAATTTTCTTGCCTCTTCCTCACGACCTTTTg                                                                          <  2:130478/143‑1 (MQ=255)
                                                                       ttccCGGGTCGTTATTCTCTTTATCGCCATACCAGATGCGTTCCGTTCCGACCTTTTTCCCACGGTGCAAAATTTGCACC‑GTAGTAAAGTTTTGTTGCCCCCAACGCAGTAATTTTCTTGCCTCTTCCTCACGACCTTTTGCa                                                                        <  2:1018032/143‑1 (MQ=255)
                                                                         cccAGGTTGATAGTTTCTTTCTCGCCATACCAGATGCGTTCCGTTCCGACCTTCTTCCCACGGTGCAAAATCTGCACT‑GTAGTAAAGTTTTGTTGCCCCCAACGCAGTAATTTTCTTGCCTCTTCCTCCCGACCTTTTGCACt                                                                      <  1:274407/143‑1 (MQ=255)
                                                                                      tttCTTTATCGCCATACCAGATGCGTTCCGTTCCGACCTTTTTCCCACGGTGCAAAATTTGCACC‑GTAGTAAAGTTTTGTTGCCCCCAACGCAGTAATTTTCTTGCCTCTTCCTCACGACCTTTTGCACTGTCCGcctcacgt                                                         >  1:640239/1‑137 (MQ=255)
                                                                                      tttCTTTATCGCCATACCAGATGCGTTCCGTTCCGACCTTTTTCCCACGGTGCAAAATTTGCACC‑GTAGTAAAGTTTTGTTGCCCCCAACGCAGTAATTTTCTTGCCTCTTCCTCACGACCTTTTGCACTGTCAGGCCCCAtt                                                         >  1:640426/1‑143 (MQ=255)
                                                                                        tCTTTATCGCCATACCAGATGCGTTCCGTTCCGACCTTTTTCCCACGGTGCAAAATTTGCACC‑GTAGTAAAGTTTTGTTGCCCCCAACGCAGTAATTTTCTTGCCTCTTCCTCACGACCTTTTGCACTGTCAGCCCCCATTAc                                                       <  2:531527/143‑1 (MQ=255)
                                                                                          tttATCGCCATACCAGATGCGTTCCGTTCCGACCTTTTTCCCACGGTGCAAAATTTGCACC‑GTAGTAAAGTTTTGTTGCCCCCAACGCAGTAATTTTCTTGCCTCTTCCTCACGACCTTTTGCACTGTCAGCCCCCATTACCa                                                     <  2:541883/143‑1 (MQ=255)
                                                                                           ttATCGCCATACCAGATGCGTTCCGTTCCGACCTTTTTCCCACGGTGCAAAATTTGCACC‑GTAGTAAAGTTTTGTTGCCCCCAACGCAGTAATTTTCTTGCCTCTTCCTCACGACCTTTTGCACTGTCAGCCCCCATTACCAc                                                    <  1:682803/143‑1 (MQ=255)
                                                                                             aTCGCCATACCAGATGCGTTCCGTTCCGACCTTTTTCCCACGGTGCAAAATTTGCACC‑GTAGCAAAGTTTTGTTGCCCCCAACGCAGTAATTTTCTTGCCTCTTCCGCACGACCTTTTGCCCTGTCAGCCCCCCTCACCACTg                                                  >  2:293946/1‑143 (MQ=255)
                                                                                                gCCATACCAGATGCGTTCCGTTCCGACCTTTTTCCCACGGTGCAAAATTTGCACC‑GTAGTAAAGTTTTGTTGCCCCCAACGCAGTAATTTTCTTGCCTCTTCCTCACGACCTTTTGCACTGTCAGCCCCCATTACCACTGCaa                                               >  1:1046855/1‑143 (MQ=255)
                                                                                                 ccATACCAGATGCGTTCCGTTCCGACCTTTTTCCCACGGTGCAAAATTTACACC‑GTAGGAAAGTTTTGTTGCCCCCAACGCAGTAATTTTCCTACCTATTCCACACGACCCTCGGCACAGACACCCCCCATTACAAAAGCAAt                                              >  2:1042760/1‑143 (MQ=255)
                                                                                                    tACCAGATGCGTTCCGTTCCGACCTTTTTCCCACGGTGCAAAATTTGCACC‑GTAGTAAAGTTTTGTTGCACCCAACGCAGTACTTTTCTTGCCTCTTCCTCCCGACCTTTTGCACTGTCAGCACCCCTTACCACCACCATgag                                           >  2:174350/1‑143 (MQ=255)
                                                                                                        gggggCGTTCCGTTCCGACCTGTTTCCCACGGTGCAAAATTTGCACC‑GTAGTAAAGTTTTGTTGCCCCCAACGCAGTAATTGTCTTGCCTCTTCCTCACGACCTTTTGCACTGTCAGCCCCCATTACCACTGCAATGAgacga                                       <  2:508120/139‑1 (MQ=255)
                                                                                                          aTGCGTTCCGTTCCGACCTTTTTCCCACGGTGCAAACTTTGCACC‑GTAGTAAAGTTTTGTTGCCCCCAACGCAGTAATTTTCTTGCCTCTTCCTCACGACCTTTTGCACTGTCAGCCCCCATTACCACTGCAATGAGacgacg                                     <  1:1242696/143‑1 (MQ=255)
                                                                                                                tccgttccgACCTTTTTCCCACGGTGCAAAATTTGCACC‑GTAGTAAAGTTTTGTTGCCCCCAACGCAGTAATTTTCTTGCCTCTTCCTCACGACCTTTTGCACTGTCAGCCCCCATTACCACTGCAATGAGAAGACGGTG‑cac                              >  1:361628/1‑141 (MQ=255)
                                                                                                                  cgttccgTCCTTGTTCCCACGGTGCAAAATTCGCACC‑GTAGTTACGTTTTGTTGCCCCCACCGCAGTAATTTTCTTGCCTCTTCCTCTCGACCTTTTCCACTGTCATCCCCCGTTTCCACTGCCATGAGACGACGCTG‑CCCAt                            <  2:638680/143‑1 (MQ=255)
                                                                                                                     tccgACCTTTTTCCCACGGTGCAAAATTTGCACC‑GTAGTAAAGTTTTGTTGCCCCCAACGCAGTAATTTTCTTGCCTCTTCCTCACGACCTTTTGCACTGTCAGCCCCCATTACCACTGCAATGAGACGACGCTG‑CCCGTCTa                         <  1:561497/143‑1 (MQ=255)
                                                                                                                      ccgACCTTTTTCCCACGGTGCAAAATTTGCACC‑GTAGTAAAGTTTTGTTGCCCCCAACGCAGTAATTTTCTTGCCTCTTCCTCACGACCTTTTGCACTGTCAGCCCCCATTACCACTGCAATGAGACGACGCTG‑CCCATCTAc                        <  1:1106626/143‑1 (MQ=255)
                                                                                                                      ccgACCTTTTTCCCACGGTGCAAAATTTGCACC‑GTAGTAAAGTTTTGTTGCCCCCAACGCAGTAATTTTCTTGCCTCTTCCTCACGACCTTTTGCACTGTCAGCCCCCATTACCACTGCAATGAGACGACGCTG‑CCCATCTAc                        <  1:708913/143‑1 (MQ=255)
                                                                                                                        gACCTTTTTCCCACGGTGCAAAATTTGCACC‑GTAGTAAAGTTTTGTTGCCCCCAACGCAGTAATTTTCTTGCCTCTTCCTCACGACCTTTTGCACTGTCAGCCCCCATTACCACTGCAATGAGACGACGCTG‑CCCATCTACAg                      >  1:171484/1‑143 (MQ=255)
                                                                                                                          ccTTTTTCCCACGGTGCAAAATTTGCACC‑GTAGTAAAGTTTTGTTGCCCCCAACGCAGTAATTTTCTTGCCTCTTCCTCACGACCTTTTGCACTGTCAGCACACGTGCGCACCGCAATGAGACGACGCTG‑CCCATCTACAGcc                    >  2:562336/1‑143 (MQ=255)
                                                                                                                            tttttCCCACGGTGCAAAATTTGCACC‑GTAGTAAAGTTTTGTTGCCCCCAACGCAGTAATTTTCTTGCCTCTTCCTCACGACCTTTTGCACTGTCAGCCCCCATTACCACTGCAATGAGACGACGCTG‑CCCATCTACAGCCGa                  >  1:197552/1‑143 (MQ=255)
                                                                                                                                   tccGGTGCAAATTTTGCACC‑GTAGTAAAGTTTTGTTGCCCCCAACGCAGTAATTTTCTTGCCTCTTCCTCACGACCTTTTGCACTATCAGCCCCCATTACCACGGCAATGAGACGACGCTG‑CCCATCTACAGCCGAAGCaccg           >  2:254546/3‑140 (MQ=255)
                                                                                                                                   taCGGTGCAAATTTTGCACC‑GTAGTAAAGTTTTGTTGCCCCCAACGCAGTACTTTTCTTGCCTCTTCCTCACGCCCTTTTGCACTGTCAGCCCCCATTACCCCTCCAATGAGACGACGCTG‑CCCACGTAAAGCCGAAGCCATg           >  2:87513/2‑143 (MQ=255)
                                                                                                                                   taCGGTGCAAATTTTGCACC‑GTAGTAAAGTTTTGTTGCCCCCAACGCAGTAATTTTCTTGCCTCTTCCTCACGCCCTTTTGCACTGTCAGCCCCCCTCACAGTAGTAATGCGAC‑ACACCGCCCCATCTACCGGCGAAGCAATg           >  2:1239120/2‑143 (MQ=255)
                                                                                                                                   cgcGGTGCAAATCTTGCACC‑GTAGTAAAGTTTTGTTGCCCCCAACGCAGTCTCTTTCTTGCCTCTTCCTCACGACCTTCTGCACTGTCAGCCCCCATCACCACTGCAATGAGACGACGCTG‑CCCATCTACAGCCGAAGCAATg           <  1:447284/141‑1 (MQ=255)
                                                                                                                                   cACGGTGCAAAATTTGCACC‑GTAGTAAAGTTTTGTTGCCCCCAACGCAGTAATTTTCTTGCCTCTTCCTCACGACCTTTTGCACTGTCAGCCCCCATTACCACTGCAATGAGACGACGCTG‑CCCATCTACAGCCGAAGCAATg           >  1:137798/1‑143 (MQ=255)
                                                                                                                                   cACGGTGCAAAATTTGCACC‑GTAGTAAAGTTTTGTTGCCCCCAACGCAGTAATTTTCTTGCCTCTTCCTCACGACCTTTTGCACTGTCAGCCCCCATTACCACTGCAATGAGACGACGCTG‑CCCATCTACAGCCGAAGCAATg           >  1:1159732/1‑143 (MQ=255)
                                                                                                                                   cACGGTGCAAAATTTGCACC‑GTAGTAAAGTTTTGTTGCCCCCAACGCAGTAATTTTCTTGCCTCTTCCTCACGACCTTTTGCACTGTCAGCCCCCATTACCACTGCAATGAGACGACGCTG‑CCCACCTACAGCCGAAGCAATg           >  2:1082563/1‑143 (MQ=255)
                                                                                                                                          cAAAATTTGCACC‑GTAGTAAAGTTTTGTTGCCCCCAACGCAGTAATTTTCTTGCCTCTTCCTCACGACCTTTTGCACTGTCAGCCCCCATTACCACTGCAATGAGACGACGCTG‑CCCATCTACAGCCGAAGCAATGAGATTaa    >  1:974217/1‑143 (MQ=255)
                                                                                                                                            aaaTTTGCACC‑GTAGTAAAGTTTTGTTGCCCCCAACGCAGTAATTTTCTTGCCTCTTCCTCACGACCTTTTGCACTGTCAGCCCCCCTTACCACTGCAATGAGACGACGCTG‑CCCATCTACAGCCGAAGCAATGAGATTAAAc  >  2:565909/1‑143 (MQ=255)
                                                                                                                                              |                                                                                                                                              
ATCAAGGGTATATTTGGCTTTGATATGTGGAATTTCGGCTTTCGGTAGCACCATCCAGAACTCTTGTTCCGTTCCCAGGTCGATATTTTCTTTATCGCCATACCAGATGCGTTCCGTTCCGACCTTTTTCCCACGGTGCAAAATTTGCACC‑GTAGTAAAGTTTTGTTGCCCCCAACGCAGTAATTTTCTTGCCTCTTCCTCACGACCTTTTGCACTGTCAGCCCCCATTACCACTGCAATGAGACGACGCTG‑CCCATCTACAGCCGAAGCAATGAGATTAAAC  >  NC_000913/2081546‑2081828

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑