| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_000913 | 2,132,787 | A→C | 100% | I204S (ATC→AGC) | wcaA ← | putative colanic acid biosynthesis glycosyl transferase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 2,132,787 | 0 | A | C | 100.0% | 158.1 / ‑6.7 | 54 | I204S (ATC→AGC) | wcaA | putative colanic acid biosynthesis glycosyl transferase |
| Reads supporting (aligned to +/- strand): ref base A (0/0); major base C (28/25); minor base G (1/0); total (29/25) | |||||||||||
| Rejected as polymorphism: E-value score below prediction cutoff. | |||||||||||
| Rejected as polymorphism: Frequency below/above cutoff threshold. | |||||||||||
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. | |||||||||||
TCTGGTAGAGGGTAAACAGCTGATATTTTTTGCTGGCGCGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGATCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCG‑GTATCGAACAAACACTCTTTGAAACGCCATGCCCAGGTAAAGACCTGGTT > NC_000913/2132650‑2132926 | gcgtgTAGAGGGTAAACAGCTGATATTTTTTGCTGGCGCGGTCGAATTTGTCTTTGTGCTTGCGGTAACAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGc < 2:692550/139‑1 (MQ=255) cTGGTAGAGGGTAAACAGCTGATATTTTTTGCTGGCGCGGTCGAATTTGTCTTTGTGCTTGCGGGAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCg > 1:201324/1‑143 (MQ=255) tAGAGGGTAAACAGCTGATATTTTTTGCTGGCGCGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGTAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGc < 1:670558/143‑1 (MQ=255) agagGGTAAACAGCTGATATTTTTTGCTGGCGCGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGcc > 1:11676/1‑143 (MQ=255) ggTAAACAGCTGATATTTTTTGCTGGCGCGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGGTCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCccctg > 1:45080/1‑142 (MQ=255) ggTAAACAGCTGATATTTTTTGCTGGCGCGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCtctt > 2:1226840/1‑143 (MQ=255) aaaCAGCTGATATTTGTGGCTGGCGCGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTa < 1:150499/143‑1 (MQ=255) aaaCAGCGGATATTTTTGGCTGGCGCGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGCAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTa < 1:80895/143‑1 (MQ=255) aaaGCTGATATTTTTTGCTGGCCGGTTCGAATGTGTTTTTGTGGTTGCGGTAAAAGTGGAAGTACACGGAGAATGGTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCGGCGTCGCCTCTTCTACt < 2:815426/141‑1 (MQ=255) aCAGCTGATATTTTTTGCTGGCGCGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGGTCTGCATTTCACCGTGATTGCTATGCAGGCTCTGCGTCGCCCCTTCTCCt > 1:430542/1‑143 (MQ=255) cAGCTGATATTTTTTGCTGGCGCGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACtt < 1:340877/143‑1 (MQ=255) gCTGATAGTTTTTGCGGGCGCGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTc < 1:783824/143‑1 (MQ=255) cTGATATTTTTTGCTGGCGCGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTcc > 1:952149/1‑143 (MQ=255) tGATATTTTTTGCTGGCGCGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCa > 1:1248791/1‑143 (MQ=255) tGATATTTTTTGCTGGCGCGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACGTGCCa > 1:301740/1‑143 (MQ=255) tGATATTTTTTGCTGGCGCGGTCGAATTTGTCTTTGTGCTGGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCa < 2:249260/143‑1 (MQ=255) atatTTTTTGCTGGCGCGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACg > 1:842697/1‑143 (MQ=255) ttttttGCTGGCGCGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGTGGTGTTCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGtt > 1:1076337/1‑143 (MQ=255) ttttttGCTGGCGCGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGtt > 1:70408/1‑143 (MQ=255) tttttGCTGGCGCGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGCGGTGATCTGCATTTCACCGTGATTGATATGCAGGGTCTGCGTCGCCCCTTCTCCTTCCCACGGTTc > 1:378641/1‑143 (MQ=255) gcgcGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTc > 1:622622/1‑143 (MQ=255) cgGTCGAATTTGTCCTTGTGCTTGCGGTAGAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTccac < 1:369977/143‑1 (MQ=255) gTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCACGCTCTGCGTCGCCTCTCCTACTTTCCACGGTTCGCCGTACCccccca > 2:530159/1‑143 (MQ=255) gTCGAATTTGTCTTTGCGCCTGCGCTCGCAGTGGAAGTCCCCGGAGAATTTTTTCGGTGACGTGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTccacca < 1:645600/143‑1 (MQ=255) gAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCCCGGTTCGCCGTCCTccaccaccc > 1:1068312/1‑142 (MQ=255) ttGGCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCg < 1:871277/143‑1 (MQ=255) tttGTGCTTGCGGTAAAAGTGGAAGTACCCGGATCATGTTTTGGATGTCGTGGTGATCTGCATTTCAACGTGATTGAGATGCAGGCTGTGGGTAGCATCTTTTAGTATCCAGTGTTCGTCGTATTCCAACACCGACCGCAGGa > 2:1045948/1‑143 (MQ=255) tttGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCTGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAggc > 1:1009164/1‑142 (MQ=255) tttGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGa > 1:310163/1‑143 (MQ=255) tttGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATACAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCCCCATCCGCAggc > 1:425570/1‑142 (MQ=255) tggtgCTCGCGGTAGTAGTGGACGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACGTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGaa < 2:1077839/141‑1 (MQ=255) gCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGCTg > 2:230731/1‑143 (MQ=255) ttGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTc > 2:535954/1‑143 (MQ=255) tAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATc > 1:310690/1‑143 (MQ=255) aaGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCtg < 1:1162269/143‑1 (MQ=255) tGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCCGGAAGATGTCGTAATCCTGTGc > 1:554547/1‑143 (MQ=255) tGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCGCTGCGTCGCCTATTCTACTTTCCACGGATCGCCGTACTCCACCACCACCCGAAGAAAGATGTCGTAAACCtggcc > 2:125661/1‑140 (MQ=255) aaGTACCGGGGGTATGTTTGCGGCGACGAGGTGATCGGCATTTCACCGTGTTTGAGTGGCAGGCTCTGCGTCGCCTCTGCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGc < 2:137491/143‑1 (MQ=255) cccGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCa < 1:53286/143‑1 (MQ=255) gagaATTTTTTCGGTGACGTGGTGATCTGCATTTCACCGTGATTGTTATGCAGGCTCTGCGTCGCCTCGTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTc < 1:376188/143‑1 (MQ=255) agtgttttctCGATGACGAGGTGGTCTGCCTTCCAGCGTGATTGATATGCAGCCTCTGCGTCGACGCGTCTAGTTGCCACGGGTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCg < 2:505268/139‑1 (MQ=255) ttttCGGTGACGTGGTGCTCTGCATTTCACCGTGATTGATGTGCCGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCG‑GTATCg < 2:721739/143‑1 (MQ=255) tttCGGTGCCGAGGTGATCTGCATTTCACCGTGCTTGCTATCCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGACGCTGTCGTAATCCTGTGCCGCTTTCAGTTCG‑GTATCGa < 1:484661/143‑1 (MQ=255) ttCGGTGAGGGGGTGATCTGCATTTCACCGGGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATTCTGTGCCGCTTTCAGTTCG‑GTATCGaa < 2:25901/143‑1 (MQ=255) cGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCT‑GTATCGaaca < 1:1010527/143‑1 (MQ=255) gTGACGAGGTGATCTGCATTTCACCGTTATTGATGTGCAGGCTCTGCGTCGCCTCTTCTACTTTCCCCTGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCG‑GTATCGaacaaa < 2:1036081/143‑1 (MQ=255) gTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCG‑GTATCGaacaaa > 1:991186/1‑143 (MQ=255) aTCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCG‑GTATCGAACAAACACTCTTTgca > 1:697358/1‑141 (MQ=255) gCATTTCACCGTGATTGATATGAAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGCACTCCACCACCATCCGCAGAAAGATGTCGTAATCCTGTGCCGCTTTCAGTTGGCGTGT‑GAGCAAACACTCTTTGacaggc > 2:1025200/1‑138 (MQ=255) ccGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCG‑GTATCGAACAAACACTCTTTGAAACGCCATGCCCa < 1:1008502/143‑1 (MQ=255) tGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCG‑GTATCGAACAAACACTCTTTGAAACGCCATGCCCAGGTAAAGACCTg < 1:670054/143‑1 (MQ=255) cAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCGGGAAGATGTCGTAATCCTGTGCCGCTTTCCGTTCG‑GTATCGAACAAACACTCTTTGAACCGCCATGCCCAGCTAAAGCCCTgct > 1:1227927/1‑141 (MQ=255) cAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCG‑GTATCGAACAAACACTCTTTGAAACGCCATGCCCAGGTAAAGACCTGGt > 2:546048/1‑143 (MQ=255) aGGCTCTGCGTCGCCTCTTCTACTGTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCG‑GTATCGAACAAACACTCTTTGAAACGCCATGCCCAGGTAAAGACCTGGtt < 1:1226840/143‑1 (MQ=255) | TCTGGTAGAGGGTAAACAGCTGATATTTTTTGCTGGCGCGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGATCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCG‑GTATCGAACAAACACTCTTTGAAACGCCATGCCCAGGTAAAGACCTGGTT > NC_000913/2132650‑2132926 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |