| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_000913 | 2,172,350 | A→T | 7.3% | L175Q (CTG→CAG) | gatD ← | galactitol‑1‑phosphate 5‑dehydrogenase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 2,172,350 | 0 | A | T | 7.3% | 143.4 / 4.5 | 56 | L175Q (CTG→CAG) | gatD | galactitol‑1‑phosphate 5‑dehydrogenase |
| Reads supporting (aligned to +/- strand): ref base A (26/25); new base T (2/2); total (28/28) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.68e-01 | |||||||||||
CTCTGCATTTGCGGCGCGCTCATTTCGCTACTGTTAAATGTTTGCATCGCACCGAAAGATTTTGCCAGTGCCAGTTTTTCTGAACTAATGTCGATCGCCGTCACACTCTTTGCTCCCAGCGCGACAGCGCACTGAATGGCCAGCAGGCCAATGGTTCCGGCACCAATAATAATAACGTTTTTATTCTCACAACCTTGCGCTAAATGAAAAGCATGCAGACCAACGGTAATCGGCTCAATAAAAGCCCCATCCTCAATAGGCATATCCGTGGGTAGAGCAAAGAC > NC_000913/2172209‑2172492 | ctctGCATTCGCGGCGCGCTCCTTTCGCTACTGTTAGCTGTTTGCATCGCACCGAAAGATTTTGCCAGTGCCAGTTTTTCTGAACTAAGGTCGATCGCCGTCACACTCTTTGCTCCCAGCGCGACAGCGCACTGAATGGCcag < 2:250013/143‑1 (MQ=255) ctGCATTTGCGGCTCGCTCATCTCGCTACTGTTAAATGTTTGCATCGCACCGAAAGATTTTGCCAGTGCCAGTTTTTCTGAACTAATGTCGATCGCCGTCACACTCTTTGCTCCCAGCGCGACAGCGCACTGAATGGCcagca < 2:350123/143‑1 (MQ=255) gcggcgCGCTCATTTCGCTACTGTTAAATGTTTGCATCGCACCGAAAGATTTTGCCAGTGCCAGTTTTTCTGAACTAATGTCGATCGCCGTCACACTCTTTGCTCCCAGCGCGACAGCGCACTGAATGGCCTGCTGGCCattc < 2:928314/143‑4 (MQ=255) gcggcgCGCTCATTTCGCGACTGTTAAATGGTTGCATCGCACCGAAAGATTTTGCCAGTGCCAGTTTTTCTGAACTAATGTCGATCGCCGTCACACTCTTTGCTCCCAGCGCGACAGCGCACTGAATGGCCAGCAGGCCAATg < 1:981219/143‑1 (MQ=255) gcgcgcTCATTTCGCTACTGTTAAATGTTTGCATCGCACCGAAAGATTTTGCCAGTGCCAGTTTTTCTGAACTAATGTCGATCGCCGTCACACTCTTTGCTCCCAGCGCGACAGCGCACTGAATGGCCTGCTGGCCattcagt < 2:468885/143‑7 (MQ=255) cgcTCATTTCGCTACTGTTAAACGCTTGCATCGCACCGAAAGATTTTGCCAGTGCCAGTTTTTCTGAACTAATGTCGATCGCCGTCACACTCTTTGCTCCCAGCGCGACAGCGCACTGAATGGCCAGCAGGCCAATGGTTCCg < 1:384941/143‑1 (MQ=255) cTCATTTCGCTACTGTTAAATGTTTGCATCGCACCGAAAGATTTTGCCAGTGCCAGTTTTTCTGAACTAATGTCGATCGCCGTCACACTCTTTGCTCCCAGCGCGACAGCGCACTGAATGGCCAGCAGGCCAATGGTTCCGGc > 2:226732/1‑143 (MQ=255) cTCATTTCGCTACTGTTAAATGTTTGCATCGCACCGAAAGATTTTGCCAGTGCCAGTTTTTCTGAACTAATGTCGATCGCCGTCACACTCTTCGCTCCCAGCGCGACAGCGCACTGCATGGCCAGCAGCCCAACGGCTCCGGc > 2:239014/1‑143 (MQ=255) tCATTTCGCTACTGTTAAATGTTTGCATCGCACCGAAAGATTTTGCCAGTGCCAGTTTTTCTGAACTAATGTCGATCGCCGTCACACTCTTTGCTCCCAGCGCGACAGCGCACTGAATGGCCAGCAGGCCAATGGTTCCGGCa > 1:501390/1‑143 (MQ=255) tttCGCTACTGTTAAATGTTTGCATCGCACCGAAAGATTTTGCCAGTGCCAGTTTTTCTGAACTAATGTCGATCGCCGTCACACTCTTTGCTCCCAGCGCGACAGCGCACTGAATCGCCAGCAGGCCAATGGTTCCGGCAccc > 2:643655/1‑142 (MQ=255) gCTACTGTTAAATGTTTGCATCGCACCGAAAGATTTTGCCAGTGCCAGTTTTTCTGAACTAATGTCGATCGCCGTCACACTCTTTGCTCCCAGCGCGACAGCGCACTGAATGGCCAGCAGGCCAATGGTTCCGCCACCcataa > 2:248886/1‑143 (MQ=255) ccaCTGGTAAATGTTTGCATCGCACCGAAAGATTTTGCCAGTGCCAGTTTTTCTGAACTAATGTCGATCGCCGTCACACACATCGCTCCTAGCGCGACAGCGCACTGAATGGCCAGCAGGCCAACGGATCCGGCACCactaat > 2:1263624/3‑143 (MQ=255) cTACTGTTAAATGTTTGCATCGCACCGAAAGATTTTGCCAGTGCCAGTTTTTCTGAACTAATGTCGATCGCCGTCACACTCTTTGCTCCCAGCGCGACAGCGCACTGAATCGCAAGCCGGCCAATGGTTCCGGCACAactaat > 2:804497/1‑143 (MQ=255) ttAAATGTTTGCATCGCACCGAAAGATTTTGCCAGTGCCAGTTTTTCTGAACTAATGTCGATCGCCGTCACACTCTTTGCTCCCAGCGCGACAGCGCACTGAATGGCCAGCAGGCCAATGGTTCCGGCACCAATAATAATAAc > 1:836416/1‑143 (MQ=255) gTTTGCATCGCACCGAAAGATTTTGCCAGTGCAAGTTATCTGGTATTAATGTGGATCGCCGTCACACTCTTTGTGGTCATCGCGATAGTGCACTGAATGTCGAGCAGTCTAATGTTTCCGGCAGCAATAAAAATAACGttttt > 2:153408/1‑143 (MQ=255) tttGCATGGCACCGAATGATTTTGCCAGTGCCAGTGTTTCTGAACTAATGTCGATCGCCGTCACACTCTTTGCTCCCAGCGCGACAGCGCACTGAATGGCCAGCAGGCCAATGGTTCCGGCACCAATAATAATAACGTTTTTa < 2:707977/143‑1 (MQ=255) gCATCGCACCGAAAGATTTTGCCAGTGCCAGTTTTTCTGAACTAATGTCGATCGCCGTCACACTCTTTGCTCCCAGCGCGACAGCGCACTGAATGGCCAGCAGGCCAATGGTTCCGGCACCAATAATAAGAACGTTTTTATtc > 1:1165444/1‑143 (MQ=255) cATCGCACCGAACGATTTTGCCAGTGCCAGTTTTTCTGAACTAATGTCGATCGCCGTCACACTCTTTGCTCCCAGCGCGACAGCGCACTGAATGGCCAGCAGGCCAATGGTTCCGGCACCAATAATAATAACGTTTTTATtct < 1:220376/143‑1 (MQ=255) cATCGCACCGAAAGATTTTGCCAGTGCCAGTTTTTCTGAACTAATGTCGATCGCCGTCACACTCTTTGCTCCCAGCGCGACAGCGCACTGAATGGCCAGCAGGCCAATGGTTCCGGCACCAATAATAATAACGTTTTTATgct > 1:334570/1‑143 (MQ=255) aTCGCACCGAAAGAGTTTGCCAGTGCCAGTTGTTCTCAACTAATGTCGACCGCCGTCACACTCTTTGCTCCCAGCGCGACAGCGCACTGAATGGCCAGCAGGCCAATGGTTCCGGCACCAATAATAATAACGTTTTTATcctc < 2:222957/143‑1 (MQ=255) tCGCACCGAAAGATTTTGCCAGTGCCAGTTTTTCTGAACTAATGTAGATCGCCGTCATGTTCTGTGGTCACACACTTACAGCGCACTGAATGGCCAGCAGGCCAATGGTTCCGGCACCAATAATAATAACGTTTTTATTCTca > 1:295471/1‑143 (MQ=255) cGCACCGATAGAGTGTGCCCGTGCCAGTCTTTCTGCACTAATGTCGATCGCCGTCCCACTCTTTGCTCCCAGCGCGACAGCGCACTGAATGGCCAGCAGGCCAATGGTTCCGGCACCAATAATAATAACGTTTTTATTCTcac < 2:613430/143‑1 (MQ=255) gCACCGAAAGATTTTGCCAGTGCCAGTTTTTCTGAACTAATGTCGATCGCCGTCACACTCTTTGCCCCCAGCGCGACAGCGCACTGAATGGCCAGCAGGCCAATGGCTCCGGCACCACTAATAATCACGTTTTTATACCcaca > 2:84844/1‑143 (MQ=255) cACCGAAAGATTTTGCCAGTGCCAGTTTTTCTGAACTAATGTCGATCGCCGTCACACTCTTTGCTCCCAGCGCGACAGCGCACTGAATGGCCAGCAGGCCAATGGTTCCGGCACCAATAATAATAACGTTTTTATTCTCACaa < 1:818806/143‑1 (MQ=255) cACCGAAAGATTTTGCCAGTGCCAGTTTTTCTGAACTAATGTCGATCGCCGTCACACTCTTTGCTCCCAGCGCGACAGCGCACTGAATGGCCAGCAGGCCAATGGTTCCGGCACCAATAATAATAACGTTTTTATTCTCACaa < 1:431544/143‑1 (MQ=255) cACCGAAAGATTCTGCCAGTGCCAGTTTTTCTGAACTAATGTCGATCGCCGTCACACTCTTTGCTCCCAGCGCGACAGCGCACTGAATGGCCAGCAGGCCAATGGTTCCGGCACCAATAATAATAACGTTTTTATTCTCACaa < 2:475556/143‑1 (MQ=255) aGATTTTGCCAGTGCCAGTTTTTCTGAACTAATGTCGATCGCCGTCACACTCTTTGCTCCCAGCGCGACAGCGCACTGAATGGCCAGCAGGCCAATGGTTCCGGCACCAATAATAATAACGTTTTTATTCTCACAACCTTgcg < 1:766865/143‑1 (MQ=255) ttttGCCAGTGCCAGTTTTTCTGAACTAATGTCGATCGCCGTCACACTCTTTGCTCCCAGCGCGACAGCGCACTGAATGGCCAGCAGGCCAATGGTTCCGGCACCAATAATAATAACGTTTTTATTCTCACAACCTTGCGCTa > 1:447552/1‑143 (MQ=255) agtgccagtTTTTCTGAACTAATGTCGATCGCCGTCACACTCTTTGCTCCCAGCGCGACAGCGCACTGAATGGCCAGCAGGCCAATGGTTCCGGCACCAATAATAATAACGTTTTTATTCTCACAACCTTGCGCTAAATGaaa < 1:261164/143‑1 (MQ=255) gtgccaggTTTTCTGAACTAATGTCGATCGCCGTCACACTCTTTGCTCCCAGCGCGACAGCGCACTGAATGGCCAGCAGGCCAATGGTTCCGGCACCAATAATAATAACGTTTTTATTCTCACAACCTTGCGCTAAATGaaaa < 1:338736/143‑1 (MQ=255) ggtggtgcTGAACTAGTGGTGTGCGCAGTCACACGCGTTGCTGCCAGCGCGACAGCGCACTGAATGGCCAGCAGGCCGATGGTTCCGGCACCAATAATAATAACGTTTTTATTCTCACAACCTTGCGCTAAATGAAAAGCATg < 2:507297/136‑1 (MQ=255) tgcTGAACTAATGCCGATCGCCGTCACACTCTTTGCCCCCAGCGGGTCAGCGCACTGAATGGCCAGCAGGCCAATGGTTCCGGCACCAATAATAATAACGTTTTTATTCTCACAACCTTGCGCTAAATGAAAAGCATGCAGAc < 2:836416/141‑1 (MQ=255) tGAACTAATGTCGATCGCCGTCACACTCTTTGCTCCCAGCGCGACAGCGCACTGAATGGCCAGCAGGCCAATGGTTCCGGCACCAATAATAATAACGTTTTTATTCTCACAACCTTGCGCTAAATGAAAAGCATGCAGACCaa > 2:279219/1‑143 (MQ=255) gAACTAATGTCGATCGCCGTCACACTCTTTGCTCCCAGCGCGACAGCGCACTGAATGGCCAGCAGGCCAATGGTTCCGGCACCAATAATAATAACGTTTTTATTCTCAAAACCTTGCGCTAAATGAAACGCATGAAGACCCAc > 1:494903/1‑143 (MQ=255) tGTCGATCGCCGTCACACTCTTTGCTCCCAGCGCGACAGCGCACTGAATGGCCAGCAGGCCAATGGTTCCGGCACCAATAATAATAACGTTTTTATCCCCACAACCTTCGCCTAAATGAAACGCAGGAAGCCCAACGGaaacg > 2:869461/1‑141 (MQ=255) gTCGATCGTCGTCGGACTCTTTGCTCCCAGCGCGACAGCGCACTGAATGGCCAGCAGGCCAATCGTTCCGGCACCAATAATAATAACGTTTTTAGTCTCACAACCTTGCGCTAAATGAAAAGCATGCAGACCAACGGTAATCg < 2:917684/143‑1 (MQ=255) cGATCGCCGTCACACTCTTTGCTCCCAGCGCGACAGCGCACTGAATGGCCAGCAGGCCAATGGTTCCGGCACCAATAATAATAACGTTTTTATTCTCACAACCTTGCGCTAAATGAAAAGCATGCAGACCAACGATAATAGGc > 2:396508/1‑143 (MQ=255) gATCGCCGTCACACTCTTTGCTCCCAGCGCGACAGCGCACTGAATGGCCAGCAGGCCAATGGTTCCGGCCCCAATAATAATAACGTTTTTATTCTCACAACCTTGCGCTCACTGAACA‑CCTGCAGACCAACGGTAATCCGccc > 1:146134/1‑141 (MQ=255) acacTCTTTGCTCCCAGCGCGACAGCGCACTGAATGGCCAGCAGGCCAATGGTTCCGGCACCAATAATAATAACGTTTTTATTCTCACAACCTTGCGCTAAATGAAAAGCATGCAGACCAACGGTAATCGGCTCAATAAAAGc < 1:696175/143‑1 (MQ=255) acacTCCTTGCCCCCAGCGCGACAGCGCACTGAATGGCCAGCAGGCCAATGGTTCCGGCACCAATAATAATAACGTTTTTATTCTCACAACCTTGCGCTAAATGAAAAGCATGCAGACCAACGGTAATCGGCTCAATAAAAGc < 2:800856/143‑1 (MQ=255) cacTCTTTGCTCCCAGCGCGACAGCGCACTGAATGGCCAGCAGGCCAATGGTTCCGGCACCAATAATAATAACGTTTTTATTCTCACAACCTTGCGCTAAATGAAAAGCATGCAGACGAACGGTAATCGGCTCAATAAAAGcc > 1:1129467/1‑143 (MQ=255) cctctTTGCTCCCAGCGCGACACCACACTGAATGGCCAGCAGGCCAATGGTTCCGGCACCAATAATAATAACGTTTTTATTCTCACAACCTTGCGCTAAATGAAAAGCATGCAGACCAACGGTAATCGGCTCAATAAAAGccc < 2:501390/142‑1 (MQ=255) acTCTTTGCTCCCAGCGCGACAGCGCACTGAATGGCCAGCAGGCCAATGGTTCCGGCACCAATAATAATAACGTTTTTATTCTCACAACCTTGCGCTAAATGAAAAGCATGCAGACCAACGGTAATCGGCTCAATAAAAGccc > 2:749738/1‑143 (MQ=255) ctctTTGCTCCCAGCGCGACAGCGCACTGAATGGCCAGCAGGCCAATGGTTCCGGCACCAATAATAATAACGTTTTTATTCTCACAACCTTGCGCTAAATGAAAAGCATGCAGACCAACGGTAATCGGCTCAATAAAAGcccc > 1:16512/1‑143 (MQ=255) tttGCTCCCAGCGCGACAGCGCACTGAATGGCCAGCAGGCCAATGGTTCCGGCACCAATAATAATAACGTTTTTATTCTCACAACCTTGCGCTAAATGAAAAGCATGCa < 2:675617/109‑1 (MQ=255) tttGCTCCCAGCGCGACAGCGCACTGAATGGCCAGCAGGCCAATGGTTCCGGCACCAATAATAATAACGTTTTTATTCTCACAACCTTGCGCTAAATGAAAAGCATGCa > 1:675617/1‑109 (MQ=255) tGCTCCCAGCGCGACAGCGCACTGAATGGCCAGCAGGCCAATGGTTCCGGCACCAATAATAATAACGTTTTTATTCTCACAACCTTGCGCTAAATGAAAAGCATGCAGACCAACGGTAATCGGCTCAATACAAGCCCCATCCt > 1:1182296/1‑143 (MQ=255) ccggcgcGACAGCGCACTGAATGGCCAGCAGGCCAATGGTTCCGGCACCAATAATAATAACGTTTTTATTCTCACAACCTTGCGCTAAATGAAAAGCATGCAGACCAACGGTAATCGGCTCAATAAAAGCCCCATCCTCAATa < 2:295471/140‑1 (MQ=255) gcgcGACAGCGCACTGAATGGCCAGCAGGCCAGTGGTTCCGGCACCAATAATAATAACGTTTTTATTCTCACAACCTTGCGCTAAATGAAAAGCATGCAGACCAACGGTAATCGGCTCAATAAAAGCCCCATCCTCAATAGGc < 2:885582/143‑1 (MQ=255) gcgcACTGAATGGCCAGCAGGCCAATGGTTCCGGCACCAATAATAATAACGTTTTTATTCTCACAACCTTGCGCTAAATGAAAAGCATGCAGACCAACGGTAATCGGCTCAATAAAAGCCCCATCCTCAATAGGCATATCCGt < 1:1143989/143‑1 (MQ=255) ccaaCTGGATGCCCAGCATGTCAATGGTTCCGGCACCAATAATAATAACGTTTTTATTCTCACAACCATGCGCTAAATCAAAAGCATGCAGACCAACGGTAATCGGCTCAATAAAAGCCCCATCCGCAATAGGCATATCCGTg < 2:1109004/140‑1 (MQ=255) gcACTGGATGGCCCGCAGGCCAATGGTTCCGGCACCAATAATAATAACGTTTTTATTCTCACAACCTTGCGCTAAATGAAAAGCATGCAGACCAACGGTAATCGGCTCAATAAAAGCCCCATCCTCAATAGGCATATCCGTgg < 2:164562/143‑1 (MQ=255) gcACTGAATGGCCAGCAGGCCAATGGTTCCGGCACCAATAATAATAACGTTTTTATTCTCACAACCTTGCGCTAAATGAAAAGCATGCAGACCAACGGTAATCGGCTCAATAAAAGCCCCATCCTCAATAGGCATATCCGTgg < 1:298486/143‑1 (MQ=255) cACTGAATGGCCAGCAGGCCAATGGTTCCGGCACCAATATTAATAACGTTTTTATTCTCACAACCTTGCGCTAAATGAAAAGCATGCAGACCAACGGTAATCGGCTCAATAAAAGGCACATCCTCAATAGGCATATCCGTggg > 1:105894/1‑143 (MQ=255) cattGGCCTGCTGGCCAATGGTTCCGGCACCAATAATAATAACGTTTTTATTCTCACAACCTTGCGCTAAATGAAAAGCATGCAGACCAACGGTAATCGGCTCAATAAAAGCCCCATCCTCAATAGGCATATGCGGGGGTaga > 1:792492/4‑143 (MQ=255) attGGCCTGCTGGCCAATGGTTCCGGCACCAATAATAATAACGTTTTTATTCTCACAACCTTGCGCTAAATGAAAAGCATGCAGACCAACGGTAATCGGCTCAATAAAAGCCCCATCCTCAATAGGCATATCCGTGGGTagag > 2:725312/3‑143 (MQ=255) tGGCCAGCAGGCCAATGGTTCCGGCACCAATAATAATAACGTTTTTATTCTCACAACCTTGCGCTAAATGAAAAGCATGCAGACCAACGGTAATCGGCTCAATAAAAGCCCCATCCTCAATAGGCATATCCGTGGGTAGAGCa < 2:930813/143‑1 (MQ=255) ccAGCAGGCCAATGGTTCCGGCACCAATAATAATAACGTTTTTATTCTCACAACCTTGCGCTAAATGAAAAGCATGCAGACCAACGGTAATCGGCTCAATAAAAGCCCCACCCTCAATAGGCATATCCGCGCGTAGAGCAAAg > 2:937599/1‑143 (MQ=255) cagcagGCCACTGGGTCCCGCACCAATAATAATAACGCTTTTACTCTCACAGCCTTGCGCTAAATGAAAAGCATGCAGCCCAACGGTAATCGGCTCAATAAAAGCCCCATCCTCAATAGGCATATCCGTGGGTAGAGCAAAGa < 2:937236/143‑1 (MQ=255) cagcagGCCAATGGTTCCGGCACCAATAATAATAACGTTTTTATTCTCACAACCTTGCGCTAAATGAAAAGCATGCAGACCAACGGTAATCGGCTCAATAAAAGCCCCATCCTCAATACGCATATCCCTCGGCAGAGCAAAGa > 2:256562/1‑143 (MQ=255) agcagGCCAATGGTTCCGGCACCAATAATAATAACGTTTTTATTCTCACAACCTTGCGCTAAATGAAAAGCATGCAGACCAACGGTAATCGGCTCAATAAAAGCCCCATCCTCAATAGGCATATCCGCGGGTAGAGCAAAGAc > 1:150358/1‑143 (MQ=255) | CTCTGCATTTGCGGCGCGCTCATTTCGCTACTGTTAAATGTTTGCATCGCACCGAAAGATTTTGCCAGTGCCAGTTTTTCTGAACTAATGTCGATCGCCGTCACACTCTTTGCTCCCAGCGCGACAGCGCACTGAATGGCCAGCAGGCCAATGGTTCCGGCACCAATAATAATAACGTTTTTATTCTCACAACCTTGCGCTAAATGAAAAGCATGCAGACCAACGGTAATCGGCTCAATAAAAGCCCCATCCTCAATAGGCATATCCGTGGGTAGAGCAAAGAC > NC_000913/2172209‑2172492 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |