Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,364,995 T→G 8.2% intergenic (+16/+23) nudI → / ← ais pyrimidine deoxynucleotide diphosphatase NudI/putative lipopolysaccharide core heptose(II)‑phosphate phosphatase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,364,9950TG 8.2% 136.9 / 4.0 56intergenic (+16/+23)nudI/aispyrimidine deoxynucleotide diphosphatase NudI/putative lipopolysaccharide core heptose(II)‑phosphate phosphatase
Reads supporting (aligned to +/- strand):  ref base T (22/29);  new base G (3/2);  total (25/31)
Fisher's exact test for biased strand distribution p-value = 6.47e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.24e-01

GCCAACCGAGAAGTGAAAATAAACGAAGAGTTTCAGGACTACGCGTGGGTAAAACCTGAAGATCTGGTGCATTATGATTTGAATGTCGCCACCCGAAAAACGTTACGTTTGAAAGGTCTTCTGTAACAATGATGACAGCCATTACTGGCTGTCAGATTGAAAATTTAGTGGTTAACGAATTCCCCATCCAGATAAACTTTGCCTTTTTCCACATGCATGACTAAACCATCCAGATAATCAGGTTTAAATGTCGCGTCACGCTTATCTTTAGCAATATATGTCA  >  NC_000913/2364854‑2365136
                                                                                                                                             |                                                                                                                                             
gCCAACCGAGAAGTGAAAATAAACGAAGAGTTTCAGGACTACGCGTGGGTAAAACCTGAAGATCTGGTGCATTATGATTTGAATGTCGCCACCCGAAAAACGTTACGTTTGAAAGGTCTTCTGTAACAATGATGACAGCCAtt                                                                                                                                              >  1:229615/1‑143 (MQ=255)
  cAACCGAGAAGTGAAAATAAACGAAGAGTTTCAGGACTACGCGTGGGTAAAACCTGAAGATCTGGTGCATTATGATTTGAATGTCGCCACCCGAAAAACGTTACGCTTGCAAGGTCTTCTGTAACAATGATGACAGCCATTAc                                                                                                                                            >  1:343509/1‑143 (MQ=255)
     ccGAGAAGTGAAAATAAACGAAGAGTTTCAGGACTACGCGTGGGTAAAACCTGAAGATCTGGTGCATTATGATTTGAATGTCGCCACCCGAAAAACGTTACGTTTGAAAGGTCTTCTGTAACAATGATGACAGCCATTACTgg                                                                                                                                         <  1:397523/143‑1 (MQ=255)
         gaAGTGAAAATAAACGAAGAGTTTCAGGACTACGCGTGGGTAAAACCTGAAGATCTGGTGCATTATGATTTGAATGTCGCCACCCGAAAAACGTTACGTTTGAAAGGTCTTCTGTAACAATGATGACAGCCATTACTGGCTGt                                                                                                                                     <  1:817550/143‑1 (MQ=255)
           aGTGAAAATAAACGCAGAGGTTCAGGACTACGCGTGGGTAAACGCTGATTATCCGCTGCATTATGATTTGAATGTCGCCACCCGAAAAACGTTACGTTTGAAAGGTCTTCTGTAACAATGATGACAGCCATTACTGGCTGTCa                                                                                                                                   <  1:872356/143‑1 (MQ=255)
             ggAAAATAAACGAAGAGTCTCAGGACTACGCGTGGGTAAAACCTGAAGATCGGGTGCATTATGATTTGAATGTCGCCACCCGAAACACGTTACGTTTGAAAGGTCTTCTGTAACAATGATGACAGCCAGTAATGGCTGTCatc                                                                                                                                 <  2:1243696/142‑3 (MQ=255)
                   tAAACGAAGAGTTTCAGGACTACGCGTGGGTAAAACCTGAAGATCGGGTGCATTATGATTTGAATGTCGCCACCCGAAAAACGTTACGTTTGAAAGGTATTCTGTAACAATGATGACAGCCATTACTGGCTGTCAGATTGaca                                                                                                                           >  2:345910/1‑141 (MQ=255)
                    aaaCGAAGAGTTTCAGGACTACGCGTGGGTAAAACCTGAAGATCTGGTGCATTATGATTTGAATGTCGCCACCCGAAAAACGTTACGTTTGAAAGGTCTTCTGTAACAATGATGACAGCCATTACTGGCTGTCAGATTGaaaa                                                                                                                          <  2:1180722/143‑1 (MQ=255)
                        ggagagGTTGAGGTCTATGCGTGGGTAAAACCTGAAGATCTGGTGCATTATGATTTGAATGTCGCCACGCGAAAAACGTTACGTTTGAAAGGTCTTCTGTAACAATGATGACAGCCATTACTGGCTGTCAGATTGAAAATTTa                                                                                                                      <  2:390790/141‑1 (MQ=255)
                        gAAGAGTTTCAGGACTACGCGTGGGTAAAACCTGAAGATCTGGTGCATTATGATTTGAATGTCGCCACCCGAAAAACGTTACGTTTGAAAGGTCTTCTGTAACAATGATGACAGCCATTACTGGCTGTCAGATTGAAAATTTa                                                                                                                      <  2:885520/143‑1 (MQ=255)
                        gAAGAGTTTCAGGACTACGCGTGGGTAAAACCTGAAGATCTGGTGCATTATGATTTGAATGTCGCCACCCGAAAAACGTTACGTTTGAAAGGTCTTCTGTAACAATGATGACAGCCATTACTGGCTGTCAGATTGAAAATTTa                                                                                                                      <  2:658458/143‑1 (MQ=255)
                          agagCTTCAGGACTACGCGTGGGTAAAACCTGAAGATCTGGTGCATTATGATTTGAATGTCGCCACCCGAAAAACGTTACGTTTGAAAGGTCTGCCGTAACAATGATGACAGCCATTACTGGCTGTCAGATTGAAAATTTAGt                                                                                                                    <  1:627832/143‑1 (MQ=255)
                              tttCAGGACTACGCGTGGGTAAAACCTGAAGATCTGGTGCATTATGATTTGAATGTCGCCACCCGAAAAACGTTACGTTTGAAAGGTCTTCTGTAACAATGATGACAGCCATTACTGGCTGTCAGATTGACAATTTAGTGGtt                                                                                                                >  2:540681/1‑143 (MQ=255)
                                tgaGGACGACGGGTGGGTAAAACATGAAGATCTGGTGCATTATGATTGGAGTGTCGCCACCCGAAAAACGTTACGTTGGAAAGGTCTTCTGTAACAATGATGACGGCCATTACTGGCTGTCAGATTGAAAATTTAGTGGTTaa                                                                                                              <  1:84280/141‑1 (MQ=255)
                                tCAGGACTACGCGTGGGTAAAACCTGAAGATCTGGTGCATTATGATTTGAATGTCGCCACCCGAAAAACGTTACGTTTGAAAGGTCTTCTGTAACAATGATGACAGCCATTACTGGCTGTCAGATTGAAAATTTAGTGGTTaa                                                                                                              >  1:1017091/1‑143 (MQ=255)
                                 cAGGACGACGCGTGGGTAAAACCGGAAGATCTGGTGCATTATGATTTGAATGTCGCCACCCGAAAAACGTTACGTTTGAAAGGTCTTCTGTAACAATGATGACAGCCATTACTGGCTGTCAGATTGAAAATTTAGTGGTTAAc                                                                                                             <  1:44375/143‑1 (MQ=255)
                                  aGGACTACGCGTGGGTAAAACCTGAAGATCGGGTGCATTATGATTTGAATGTCGCCACCCGAAAAACGTTACGTTTGAAAGGTCTTCTGTAACAATGATGACAGCCATTACTGGCTGTCAGATTGAAAATTTAGTGGTTAACg                                                                                                            <  1:763935/143‑1 (MQ=255)
                                    ggataagcgTGGGTCAAACCTGAAGATCTGGTGCATTATGATTTGAATGTCGCCACCCGAAAAACGTTACGTTTGAAAGGTCTTCTGTAACAATGATGACAGCCATTACTGGCTGTCAGATTGAAAATTTAGTGGTTAACGaa                                                                                                          <  1:301863/137‑1 (MQ=255)
                                    gACTACGCGTGGGTAAAACCTGAAGATCTGGTGCATTATGATTTGAATGTCGCCACCCGAAAAACGTTACGTTTGAAAGGTCTTCTGTAACAATGATGACAGCCATTACTGGCTGTCAGATTGAAAATTTAGTGGTTAACGaa                                                                                                          >  1:1109963/1‑143 (MQ=255)
                                    gACTACGCGTGGGTAAAACCTGAAGATCTGGTGCATGATGATTTGAATGTCGCCACCCGAAAAACGTTACGTTTGAAAGGTCTTCTGTAACAATGATGACAGCCATTACTGGCTGTCAGATTGAAAATTTAGTGGTTAACGaa                                                                                                          >  2:276701/1‑143 (MQ=255)
                                       tACGCGTGGGTAAAACCTGAAGATCTGGTGCATTATGATTTGAATGTCGCCACCCGAAAAACGTTACGTTTGAAAGGTCTTCTGTAACAATGATGACAGCCATTACTGGCTGTCAGATTGAAAATTTAGTGGTTAACGAATTc                                                                                                       <  2:1258628/143‑1 (MQ=255)
                                                gTAAAACCTGAAGATCTGGTGCATTATGGTTTGAATGTCGCCACCCGAAGAACGGTACGTTTGAGAGGTCTTCTGTAACAATGATGACAGCCATTACTGGCTGTCAGATGGCAAATTTAGTGGTTAACGAATTCCCCATCCAg                                                                                              <  2:516233/143‑1 (MQ=255)
                                                gTAAAACCTGAAGATCTGGTGCATTATGATTTGAATGTCGCCACCCGAAAAACGTTACGTTTGAAAGGTCTTCTGTAACAATGATGACAGCCATTACTGGCTGTCAGATTGAAAATTTAGTGGTTAACGAATTCCCCATCCAg                                                                                              >  2:1202287/1‑143 (MQ=255)
                                                         gAAGATCTGGTGCATTATGATTTGAATGTCGCCACCCGAAAAACGTTACGTTTGAAAGGTCTTCTGTAACAATGATGACAGCCATTACTGGCTGTCAGATTGAAAATTTAGTGGTTAACGAATTCCCCATCCAGATAAACttt                                                                                     >  2:215520/1‑143 (MQ=255)
                                                           aGATCTGGTGTATTATGATTTGACTGTCGCCACCCGAAAAACGTTACGTTTGAAAGGTCTTCTGTAACAATGATGACAGCCATTACTGGCTGTCAGATTGAAAATTTAGTGGTTAACGAATTCCCCATCCAGATAAACTTTGc                                                                                   <  2:130955/143‑1 (MQ=255)
                                                                  gTGCATTATGATTTGAATGTCGCCACCCGAAAAACGTTACGTTTGAAAGGTCTTCTGTAACAATGATGACAGCCATTACTGGCTGTCAGATTGAAAATTTAGTGGTTAACGAATTCCCCATCCAGATAAACTTTGCCTTTTTc                                                                            >  1:726158/1‑143 (MQ=255)
                                                                         aTGATTTGAATGTCGCCACCCGAAAAACGTTACGTTTGAAAGGTCTTCTGTAACAATGATGACAGCCATTACTGGCTGTCAGATTGAAAATTTAGTGGTTAACGAATTCCCCATCCAGATAAACTTTGCCTTTTTCCAcatgc                                                                     >  1:491109/1‑143 (MQ=255)
                                                                            aTTTGAATGTCGCCACCCGAAAAACGTTACGTTTGAAAGGTCTTCTGTAACAATGATGACAGCCATTACTGGCTGTCAGATTGAAAATTTAGTGGTTAACGAATTCCCCATCCAGATAAACTTTGCCTTTTTCCAcatgcatg                                                                  >  1:605976/1‑143 (MQ=255)
                                                                             gttGAATGTCGCCACCCGAAAAACGTTACGTTTGAAAGGTCTTCTGTAACAATGATGACAGCCATTACTGGCTGTCAGATTGAAAATTTAGTGGTTAACGAATTCCCCATCCAGATAAACTTTGCCTTTTTCCACATGCATGa                                                                 <  2:499409/142‑1 (MQ=255)
                                                                              ttGAATGTCGCCACCCGAAAAACGTTACGTTTGAAAGGTCTTCTGTAACAATGATGACAGCCATTACTGGCTGTCAGATTGACAATTTAGTGGTTATCGAATTCCCCATCCAGATAAACTTTGCCTTTTTCCACATGCATGAc                                                                >  1:174511/1‑143 (MQ=255)
                                                                                  aTGTCGCCACCCGAAAAACGTTACGTTTGAAAGGTCTTCTGTAACAATGATGACAGCCATTACTGGCTGTCAGATTGAAAATTTAGTGGTTAACGAATTCCCCATCCAGATAAACTTAGCCTTTTTCCACATTCCTGACTaaa                                                            >  2:249448/1‑143 (MQ=255)
                                                                                   tGTCGCCGCTCGCAGCACGTTCCGGTTGATGTGTCTTCTGGAACAATGAGGACAGCCATTACTGGCTGTCAGATTGAAAATTTAGTGGTTACCGAATTCCCCATCCAGATAAACTTTGCCTTTTTCCACATGCATGACTAAAc                                                           <  2:999639/143‑1 (MQ=255)
                                                                                       gCCACCCGAAAAACGTTACGTTTGAAAGGTCTTCTGTAACAATGATGACAGCCATTACTGGCTGTCAGATTGAAAATTTAGTGGTTAACGAATTCCCCATCCAGATAAACTTTGCCTTTTTCCACATGCATGACTAAACCATc                                                       <  2:445362/143‑1 (MQ=255)
                                                                                             cGAAAAACGTTACGTTTGAAAGGTCTTCTGTAACAATGATGACAGCCATTACTGGCTGTCAGATTGAAAATTTAGTGGTTAACGAATTCCCCATCCAGATAAACTTTGCCTTTTTCCACATGCATGACTAAACCATCCAGATa                                                 >  1:222903/1‑143 (MQ=255)
                                                                                              gAAAAACGTTACGTTTGAAAGGTCTTCTGTAACAATGATGACAGCCATTACTGGCTGTCAGATTGAAAATTTAGTGGTTAACGAATTCCCCATCCCGATAAACTTTGCCTTTTTCCACATGCATGACTAAACTATCCAGAtca                                                >  1:506049/1‑141 (MQ=255)
                                                                                                       tacgttTGAAAGGTCTTCTGTAACAATGATGACAGCCATTACTGGCTGTCAGATTGAAAATTTAGTGGTTAACGAATTCCCCATCCAGATAAACTTTGCCTTTTTCCACATGCATGACTAAACCATCCAGATAATCAGGTTTa                                       <  1:215520/143‑1 (MQ=255)
                                                                                                        acgttTGAAAGGTCTTCTGTAACAATGATGACAGCCATTACTGGCTGTCAGATTGAAAATTTAGTGGTTAACGAATTCCCCATCCAGATAAACTTTGCCTTTTTCCACATGCATGACTAAACCATCCAGATAATCAGGTTTaa                                      >  2:221119/1‑143 (MQ=255)
                                                                                                          gttTGAATTGTCTTCTTTAACAATGATGACAGCCATTACTGGCTGTCAGATTGAAAATTTAGTGGTTAACGAATCCCCCATCCAGATAAACTTTGCCTTTGTCCACATGCATGACTAAACCATCCAGATAATCAGGTTTAAAt                                    <  2:86666/143‑1 (MQ=255)
                                                                                                             tGAAAGGTCTTCTGTAACAATGATGACAGCCATTACTGGCTGTCAGATTGAAAATTTAGTGGTTAACGAATTCCCCATCCAGATAAACTTTGCCTTTTTCCACATGCATGACTAAACCATCCAGATAATCAGGTTTAAATGTc                                 <  2:16525/143‑1 (MQ=255)
                                                                                                              gAAAGGTCTTCTGTAACAACGATGACAGGCATTACTGGCTGTCAGATTGAAAATTTAGTGGTTAACGAATTCCCCATCCAGATAAACTTTGCCTTTTTCCACATGCATGACTAAACCATCCAGATAATCAGGTTTAAATGTcg                                <  2:199291/143‑1 (MQ=255)
                                                                                                               aagggTCTTCTGGTACAATGAGGACAGCCATTGCTGGCTGTCAGATTGAAAATTTAGTGGTTTACGAATTCCCCCTCCAGATAAACTTTGCCTTTTTCCACCTGCATGACTAAACCATCCAGATAATCAGGTTTAAATGTcgc                               <  2:1059153/140‑1 (MQ=255)
                                                                                                                      tcctGTAACCATGATGACAGCCAGTACTGGCTGTCAGACTGAAAATTGAGTGCTTAACGAATTCCCCATCCCGATAAACTGTGCCTCTTTCCACATGCATGACTAAACCATCCAGATAATCAGGTTTAAATGTCGCGTCACGc                        <  1:74459/141‑1 (MQ=255)
                                                                                                                        ctGTAACAATGATGACAGCCATTACTGGCTGTCAGATTGAAAATTTAGTGGTTAACGAATTCCCCATCCAGATAAACTTTGCCTTTTTCCACATGCATGACTAAACCATCCAGATAATCAGGTTTAAATGTCGCGTCACGCtt                      >  1:117068/1‑143 (MQ=255)
                                                                                                                         tGTAACAATGATGACAGCCATTACTGGCTGTCAGATTGAAAATTTAGTGGTTAACGAATTCCCCATCCAGATAAACTTTGCCTTTTTCCACATGCATGACTAAACCATCCAGATAATCAGGTTTAAATGTAGCGTCACGCTTa                     >  2:729459/1‑143 (MQ=255)
                                                                                                                           tcaCAGTGATGACAGCCATTACTGGCTGTCAGATTGAAAATTTAGTGATTAACGAATTCCCCATCCAGATAAACTTTGCCTTTTTCCACATGCATGACTAAACCATCCAGATAATCAGGTTTAAATGTCGCGTCACGCTTATc                   <  2:26024/141‑1 (MQ=255)
                                                                                                                           tAACAATGATGACAGCCATTACTGGCTGTCAGATTGAAAATTTAGTGGTTAACGAATTCCCCATCCAGATAAACTTTGCCTTTTTCCACATGCATGACTAAACCATCCAGATAATCAGGTTTAAATGTCGCGTCACGCTTATc                   >  2:344354/1‑143 (MQ=255)
                                                                                                                              ccatgatgTCCGCCCTTACTGGCTGTCAGATTGAAAATTTAGTGGTTAACGAATTCCCCATCCAGATAACCTTTGCCTTTTTCCACATGCATGACTAAACCATCCAGATAATCAGGTTTAAATGTCGCGTCACGCTTATCttt                <  2:605481/141‑1 (MQ=255)
                                                                                                                              cAATGATGACAGCCATTACTGGCTGTCAGATTGAAAATTTAGTGGTTAACGAATTCCCCATCCAGATAAACTTTGCCTTTTTCCACATGCATGACTAAACCATCCAGATAATCAGGTTTAAATGTCGCGTCACGCTTATCttt                <  2:917764/143‑1 (MQ=255)
                                                                                                                               aaTGATGACATCCATTACTGGCTGTCAGATTGAAAATTTAGTGGTTAACGAATTCCCCATCCAGATAAACTTTGCCTTTTTCCACATGCATGACTAAACCATCCAGATAATCAGGTTTAAATGTCGCGTCACGCTTATCTTTa               <  2:1163515/143‑1 (MQ=255)
                                                                                                                               aaTGATGACAGCCATTACTGGCTGTCAGATTGAAAATTTAGTGGTTAACGAATTCCCCATCCAGATAAACTTTGCCTTTTTCCACATGCATGACTAAACCATCCAGATAATCAGGTTTAAATGTCGCGTCAAGCGTATATTTa               >  1:486670/1‑143 (MQ=255)
                                                                                                                                 atctgaCAGCCAGTAATGGCTGTCAGATTGAAAATTTAGTGGTTAACGAATTCCCCATCCAGATAAACTTTGCCTTTTTCCACATGCATGACTAAACCATCCAGATAACCAGGTTTAAATGTCGCGTCACGCCTATCTTTAGc             >  2:252904/4‑143 (MQ=255)
                                                                                                                                    tgaCAGCCAGTAATGGCTGTCAGATTGAAAATTTAGTGGTTAACGAATTCCCCATCCAGATAAACTTTGCCTTTTTACACATGCATGACTAAACCATCCAGATAATCAGGTTTAAATGTCGCGTCACGCTTATATTTAGCAat          >  2:566807/1‑143 (MQ=255)
                                                                                                                                     gaCAGCCAGTAATGGCTGTCAGATTGAAAATTTAGTGGTTAACGAATTCCCCATCCAGATAAACTTTGCCTTTTTCCACATGCATGACTAAACCATCCAGATAATCAGGTTTAAATGTCGCGTCACGCTTATCTTTAGCAata         >  2:115576/1‑143 (MQ=255)
                                                                                                                                       ccgCCATTACTGGCTGTCAGATTGAAAATTTAGTGGTTAACGAATTCCCCATCCAGATAAACTTTGCCTTTTTCCACATGCACGACTAAACCTTCCAGATAATCAGGTTTAAATGTCGCGTCACGCTTATCTTTAGCAatata       <  1:419839/141‑1 (MQ=255)
                                                                                                                                          ccATTACTGGCTGTCAGATTGAAAATTTAGTGGTTAACGAATTCCCCATCCAGATAAACTTTGCCTTTTTCCACATGCATGACTAAACCATCCAGATAATCAGGTTTAAATGTCGCGTCACGCTTATCTTTCGCAATATATGt    >  1:1243844/1‑143 (MQ=255)
                                                                                                                                           cATTACTGGCTGTCAGATTGGAAATTTAGTGGTTAACGAATTCCCCATCCAGATAAACTTTGCCTTTTTACACATGCATGACTAAACCATCCCGATAATCAGGTTTGGATGTCGCGTCACGCTTTTCTTTGTCAATATCTGTc   >  1:827909/1‑143 (MQ=255)
                                                                                                                                            aTTACTGGCTGTCAGATTGAAAATTTAGTGGTTAACGAATTCCCCATCCAGATAAACTTTGCCTTTTTCCACATGCATGACTAAACCATCCAGATAATCAGGTTTAAATGTCGCGTCACGCTTATCTTTAGCAATATATGTCa  <  2:343509/143‑1 (MQ=255)
                                                                                                                                             |                                                                                                                                             
GCCAACCGAGAAGTGAAAATAAACGAAGAGTTTCAGGACTACGCGTGGGTAAAACCTGAAGATCTGGTGCATTATGATTTGAATGTCGCCACCCGAAAAACGTTACGTTTGAAAGGTCTTCTGTAACAATGATGACAGCCATTACTGGCTGTCAGATTGAAAATTTAGTGGTTAACGAATTCCCCATCCAGATAAACTTTGCCTTTTTCCACATGCATGACTAAACCATCCAGATAATCAGGTTTAAATGTCGCGTCACGCTTATCTTTAGCAATATATGTCA  >  NC_000913/2364854‑2365136

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑