| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_000913 | 123,050 | A→T | 86.9% | I12F (ATC→TTC) | aceE → | pyruvate dehydrogenase E1 component |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 123,050 | 0 | A | T | 86.9% | 181.9 / 12.3 | 74 | I12F (ATC→TTC) | aceE | pyruvate dehydrogenase E1 component |
| Reads supporting (aligned to +/- strand): ref base A (5/5); new base T (28/36); total (33/41) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 7.44e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
GCGGCAACTAAACGTAGAACCTGTCTTATTGAGCTTTCCGGCGAGAGTTCAATGGGACAGGTTCCAGAAAACTCAACGTTATTAGATAGATAAGGAATAACCCATGTCAGAACGTTTCCCAAATGACGTGGATCCGATCGAAACTCGCGACTGGCTCCAGGCGATCGAATCGGTCATCCGTGAAGAAGGTGTTGAGCGTGCTCAGTATCTGATCGACCAACTGCTTGCTGAAGCCCGCAAAGGCGGTGTAAACGTAGCCGCAGGCACAGGTATCA > NC_000913/122914‑123188 | gcgGCAACTAAACGTAGAACCTGTCTTATTGAGCTTTCCGGCGAGAGTTCAATGGGACAGGTTCCAGAAAACTCAACGTTATTAGATAGATAAGGAATAACCCATGTCAGAACGTTTCCCAAATGACGTGGATCCGTTCGaaa < 1:249076/143‑1 (MQ=255) cgGCAACTAAACGTAGAACCTGTCTTATTGAGCTTTCCGGCGAGAGTTCAATGGGACAGGTTCCAGAAAACTCAACGTTATTAGATAGATAAGGAATAACCCATGTCAGAACGTTTCCCAAATGACGTGGATCCGTTCGAAAc > 1:663336/1‑143 (MQ=255) gCAACTAAACGTAGAACCTGTCTTATTGAGCTTTCCGGCGAGAGTTCAATGGGACAGGTTCCAGAAAACTCAACGTTATTAGATAGATAAGGAATAACCCATGTCAGAACGTTTCCCAAATGACGTGGATCCGTTCGAAACTc > 2:22542/1‑143 (MQ=255) aaCTAAACGTATAACCTGTCTTATTGAGCTTTCCGGCGAGAGTTCAATGGGACAGGTTCCAGAAAACTCAACGTTATTAGATAGATAAGTAATAACCCATGTCAGAACGTTTCCCAAATGACGTGGATCCGTTCGAAACTcgc < 2:837087/143‑1 (MQ=255) aaCTAAACGTAGAACCTGTCTTATTGAGCTTTCCGGCGAGAGTTCAATGGGACAGGTTTAACAAAACGCAACGTTATTAGATAGATAAGGAATAACCCATGTCAGAACGTTTCCCAAATGACGTGGATCCGTTCGAAACTcgc < 1:292351/143‑1 (MQ=255) aaCTAAACGTAGAACCTGTCTTATTGAGCTTTCCGGCGAGAGTTCAATGGGACAGGTTCCAGAAAACTCAACGTTATTAGATAGATAAGGAATAACCCATGTCAGAACGTTTCCCAAATGACGTGGATCCGTTCGAACCTcgc > 2:775264/1‑143 (MQ=255) aaCTAAACGAAGAACCTGTCTTATTGAGCTTTCCGGCGAGAGTTCAATGGGACAGGTTCCAGAAAACTCAACGTTATTAGATAGATAAGGAATCACCCATGTCAGAACGTTTCCCAAATGACGTGGATCCGTTCGAAACTcgc < 2:1242701/143‑1 (MQ=255) tGTCTTATTGAGCTTTCCGGCGCGAGTTCAATGGGACAGGTTCCACAAAACTCACCGTTATTAGATAGATCAGGCATAACCCATGTCATAACGTTTCCCAAATGACCTGGATCCGTTCGAAACTCGCGACTCCCCCCAGGCgc > 2:244664/1‑142 (MQ=255) tCTTATTGAGCTTTCCGGCGAGAGTTCAATGGGACAGGTTCCAGAAAACTCAACGTTATTAGATAGATAAGGAATAACCCATGTCAGAACGTTTCCCAAATGACGTGGATCCGTTCGAAACTCGCGACTGGCTCCAGGCGATc < 1:841582/143‑1 (MQ=255) ttATTGAGCTTTCCGGCGAGAGTTCAATGGGACAGGTTCCAGAAAACTCAACGTTATTAGATAGATAAGGAATAACCCATGTCAGAACGTTTCCCAAATGACGTGGATCCGTTCGAAACTCGCGACTGGCTCCAGGCGATCGa > 2:1006800/1‑143 (MQ=255) gtcgAGCCTTCCGGCCAGAGTTCGATTGGACAGGTTCCAGAAAACTCAACGTTATTAGATAGATAAGGAATAACGCATGTCAGAACTTTTCCCAAATGACGTGGATCCGTTCGAAACTCGCGACTGGCTCCAGGCGATCGAAt < 2:1183580/140‑1 (MQ=255) ttGAGCTTTCCGGCGAGAGTTCAATGGGACAGGTTCCAGAAAACTCAACGTTATTAGATAGATAAGGAATAACCGATGTCAGAACGTTTCCCAAATGACGTGGATCCGATCGAAACTCGCGACTGGCTCCAGGCGATCGAATc > 1:669643/1‑143 (MQ=255) gtGAGCCTGCCGGCGAGGGTTCAATGGGACAGGTTCCAGAAAACTCAACGTTATTAGATAGATAAGGAATACCCCATGTCAGAACGTTTCCCAAATGACGTGGATCCGTTCGAAACTCGCGACTGGCTCCAGGCGATCGAATc < 1:1132754/142‑1 (MQ=255) ttaGCTTTCCGGCGGTACTTCAATGGGACAGGTTCCAGACAACTCAACGTTATTAGATAGATAAGGAATCTCGCATCTCAGAACGTTTCCCATATGGCGGGGATCCGTTAGAAACTCGCGTCTGGCTCCAGGCGATCGAATCg < 2:966301/141‑1 (MQ=255) tGAGCTTTCCGGCGAGAGTTCAATGGGACAGGTTCCAGAAAACTCAACGTTATTAGATAGATAAGGAATAACCCATGTCAGAACGTTTCCCAAATGACGTGGATCCGTTCGAAACTCGCGACTGGCTCCAGGCGATCGAATCg < 1:774626/143‑1 (MQ=255) tGAGCTTTCCGGCGAGAGTTCAATGGGACAGGTTCCAGAAAACTCAACGTTATTAGATAGATAAGGAATAACCCATGTCAGAACGTTTCCCAAATGACGTGGATCCGTTCGAAACTCGCGACTGGCTCCAGGCGATCGAATCg < 1:1055076/143‑1 (MQ=255) ggAGCTTTCCGGCGAGAGTTCAACGGGACAGGTTCCAGAAAACTCAACGTTATTAGATAGATAAGGAATAACCCATGTCAGAACGTTTCCCAAATGACGTGGATCCGTTCGAAAATCGCGACTGGCTCCAGGCGATCGAATCg < 1:714578/142‑1 (MQ=255) gAGCTTTCCGGCGAGAGTTCAATGGGACAGGTTCCAGAAAACTCAACGTTATTAGATAGATAAGGAATAACCCATGTCAGAACGTTTCCCAAATGACGTGGATCCGTTCGAAACTCGCGACTGGCTCCAGGCGATCGAATCgg < 1:1004419/143‑1 (MQ=255) aGCTTTCCGGCGAGAGTTCAATGGGACAGGTTCCAGAAAACTCAACGTTATTAGATAGATAAGGAATAACCCATGTCAGAACGTTTCCCAAATGACGTGGATCCGTTCGAAACTCGCGACTGGCTCCAGGCGATCGAATCGGt > 1:1266389/1‑143 (MQ=255) ttCCGGCGAGAGTTCAATGGGACAGGTTCCAGAAAACTCAACGTTATTAGATAGATAAGGAATAACCCATGTCAGAACGTTTCCCAAATGACGTGGATCCGTTCGAAACTCGCGACTGGCTCCAGGCGATCGAATCGGTCATc < 1:905178/143‑1 (MQ=255) ccGGCGAGAGTTCAATGGGACAGGTTCCAGAAAACTCAACGTTATTAGATAGATAAGGAATAACCCATGTCAGAACGTTTTTGAGACAACGAAGATCCGTTCGAAACTCGCGACTGGCTCCAGGCGATCGAATCGGTCATCCg > 1:291905/1‑143 (MQ=255) gagaGTTTAATGGGACAGGTTCCAGAAAACTCTATGTTATTAGATAGATAAGGAATAACCCATGTCAGAACGTTTCCCAAATGACGTGGATCCGTTCGAAACTCGCGACTGGCTCCAGGCGATCGAATCGGTCATCCGTgaag < 1:916012/143‑1 (MQ=255) gagaGTTCAATGGGACAGGTTCCAGAAAACTCAACGTTATTAGATAGATAAGGAATAACCCATGTCAGAACGTTTCCCAAATGACGTGGATCCGTCCGAAACTCGCGACTGGCTACAGGCGATCGAATCGATCATCCGTgacg > 2:67277/1‑141 (MQ=255) ttCAATGGGACAGGTTCCAGAAAACTCAACGTTATTAGATAGATAAGGAATAACCCATGTCAGAACGTTTCCCAAATGACGTGGATCCGTTCGAAACTCGCGACTGGCTCCAGGCGATCGCATCGGTCATCCGTGAAGAAGgt > 2:400174/1‑143 (MQ=255) ttCAATGGGACAGGTTCCAGAAAACTCAACGTTATTAGATAGATAAGGAATAACCCATGTCAGAACGTTTCCCAAATGACGTGGATCCGTTCGAAACTCGCGACTGGCTCCAGGCGATCGAATCGGTCATCAGTGAAGAAGgt > 2:928765/1‑143 (MQ=255) aTGGGACAGGTTCCAGAAAACTCAACGTTATTAGATAGATAAGGAATAACCCATGTCAGAACGTTTCCCAAATGACGTGGATCCGTTCGAAACTCGCGACTGGCTCCAGGCGATCGAATCGGTCATCCGTGAAGAAGGTGTTg < 1:844464/143‑1 (MQ=255) tGGGACAGGTTCCAGAAAGCTCAACGTTATTAGATAGATAAGGAATAACCCATGTCAGAACGTTTCCCAAATGACGTGGATCCGTTCGAAACTCGCGACTGGCTCCAGGCGATCGAATCGGTCATCCGTGAAGAAGGTGTTGa < 1:830344/143‑1 (MQ=255) ggACAGGTTCCAGAAAACTCAACGTTATTAGATAGATAAGGAATAACCCATGTCAGAACGTTTCCCAAATGACGTGGATCCGTTCGAAACTCGCGACTGGCTCCAGGCGATCGAATCGGTCATCCGTGAAGAAGGTGTTGAGc > 1:1215428/1‑143 (MQ=255) gACAGGTTCCAGAAAACTCAACGTTATTAGATAGATAAGGAATAACCCATGTCAGAACGTTTCCCAAATGACGTGGATCCGTTCGAAACTCGCGACTGGCTCCAGGCGATCGAATCGGTCATCCGTGAAGAAGGTGTTGAGCg < 1:22542/143‑1 (MQ=255) gACAGGTTCCAGAAAACTCAACGTTATTAGATAGATAAGGAATAACCCATGTCAGAACGTTTCCCAAATGACGTGGATCCGATCGAAACTCGCGACTGGCTCCAGGCGATCGAATCGGTCATCCGTGAAGAAGGTGTTGAGCg > 1:1099034/1‑143 (MQ=255) cAGGTTCCAGAAAACTCAACGTTATTAGATAGATAAGGAATAACCCATGTCAGAACGTTTCCCAAATGACGTGGATCCGTTCGAAACTCGCGACTGGCTCCAGGCGATCGAATCGGTCATCCGTGAAGAAGGTGTTGAGCGTg < 2:304040/143‑1 (MQ=255) ggTTCCAGAAAACTCAACGTTATTAGATAGATAAGGAATAACCCATGTCAGAACGTTTCCCAAATGACGTGGATCCGTTCGAAACTCGCGACTGGCTCCAGGCGATCGAATCGGTCATCCGTGAAGAAGGTGTTGAGCGTGCt < 1:426064/143‑1 (MQ=255) ggTTCCAGAAAACTCAACGTTATTAGATAGATAAGGAATAACCCATGTCAGAACGTTTCCCAAATGACGTGGATCCGATCGAAACTCGCGACTGGCTCCAGGCGATCGAATCGGTCATCCGTGAAGAAGGTGTTGAGCGTGCt < 2:380842/143‑1 (MQ=255) cAGAAAACTCAACGTTATTAGATAGATAAGGAATAACCCATGTCAGAACGTTTCCCAAATGACGTGGATCCGTTCGAAACTCGCGACTGGCTCCAGGCGATCGAATCGGTCATACGTGAAGAAGGTGTTTAGCGTGCTCAGTa > 2:460057/1‑143 (MQ=255) gAAAACTCAACGTTATTAGATAGATAAGGAATAACTCAGGTTGGAACGTTTCCCAAATGACGTGGATCCGTTCGAAACTCGCGACTGGCTCCAGGCGATCGAATCGGTCATCCGTGAAGAAGGTGTTGAGCGTGCTCAGtctc > 1:244466/1‑143 (MQ=255) gAAAACGCCACGATATTAGATAGGTAAGGAATAACCCGTGTCAGAACGTTTCCCAAATGACGTTTAGCGGTTCGAAACGCGCGACTGGCTCCAGGCGATCGAAGCTGTCATCCGTGAAGAAGGTGTTTAGCGTGCTCAGTATc < 1:270683/143‑1 (MQ=255) cTCAACGTTATTAGATAGATAAGGAATAACCCATGTCAGAACGTTTCCCAAATGACGTGGATCCGTTCGAAACTCGCGACTGGCTCCAGGCGATCGAATCGGTCATCCGTGAAGAAGGTGTTGAGCGTGCTCAGTATCTGATc < 1:699063/143‑1 (MQ=255) tCAACGTTATTAGATAGATAAGGAATAACCCATGTCAGAACGTTTCCCAAATGACGTGGATCCGATCGAAACTCGCGACTGGCTCCAGGCGATCGAATCGGTCATCCGTGAAGAAGGTGTTGAGCGTGCTCAGTATCTGATCg > 1:1160572/1‑143 (MQ=255) cAACGTTATTAGATACATAAGGAATAACCCATGTCAGAACGTTTCCCAAATGACGTGGATCCGTTCGAAACTCGCGACTGGCTCCAGGCGATCGAATCGGTCATCCGTGAAGAAGGTGTTGAGCGTGCTCAGTATCTGATCGa < 2:81940/143‑1 (MQ=255) aaCGTTATTAGATAGATACGCAATAACCCATGTCAGAACGTTTCCCAAATGACGTGGATCCGTTCGAAACTCGCGACTGGCTCCAGGCGATCGAATCGGTCATCCGTGAAGAAGGTGTTGAGCGTGCTCAGTATCTGATCGAc < 1:756887/143‑1 (MQ=255) ttattaGATAGATAAGGAAGAACCCATGTCAGAACGTTTCCCAAATGACGTGGATCCGTTCGAAACTCGCGACTGGCTCCAGGCGATCGAATCGGTCATCCGTGAAGAAGGTGTTGAGCGTGCTCAGTATCTGATCGACCAAc < 1:369609/143‑1 (MQ=255) agatagatAAGGAATAACCCATGTCAGAACGTTTCCCAAATGACGTGGATCCGTTCGAAACTCGCGACTGGCTCCAGGCGATCGAATCGGTCATCCGTGAAGAAGGTGTTGAGCGTGCTCAGTATCTGATCGACCAACtgctt > 1:770874/1‑143 (MQ=255) gatagataAGGAATAACCCATGTCAGAACGTTTCCTAAATGACGTGGGTCCGTTCGAAACTCGCGACTGGCTCCAGGCGATCGAATCGGTCATCCGTGAAGAAGGGGTCGGGCGTGCTCAGTGTCTGCTCGACCAGCtgcttg > 2:973032/1‑143 (MQ=255) atagataAGGAATAACCCATGTCAGAACGTTTCCCAAATGACGTGGATCCGTTCGAAACTCGCGACTGGCTCCAGGCGATCGAATCGGTCATCCGTGAAGAAGGTGTTGAGCGTGCTCAGTATCTGATCGACCAACtgcttgc < 2:665806/143‑1 (MQ=255) tagataAGGAATAACCCAGGTCAGAACGTTTCCCAAATGACGTGGATCCGTTCGAAACTCGCGACTGGCTCCAGGCGATCGAATCGGTCATCCGTGAAGAAGGTGTTGAGCGTGCTCAGTATCTGATCGACCAACtgcttgct < 1:357972/143‑1 (MQ=255) agataAGGAATAACCCATGTCAGAACGTTTCCCAAATGACGTGGATCCGTTCGAAACTCGCGACTGGCTCCAGCCGATCGAATCGGTCATCCGTGCAGAAGGTGTTGGGCGTGCTCACCTTCTGATCGGCCACCGGCTTGCTg > 1:1046614/1‑143 (MQ=255) agaacgAATCACCCATGTCAGAACGTTTCCCAAAGGGCGTTGATCCGTTCGAAACTCGCGACTGGCTCCAGGCGATCGAATCGGTCATCCGTGAAGAAGGTGTTGAGCGTGCTCAGTATCTGATCGACCAACTGCTTGCTGaa < 2:930190/138‑1 (MQ=255) aaaaGGAATACGCCAGGGCAGACCGTTGCCCAGATGACGTGGATCCGATCGAAACTCGCGACTGGCTCCAGGCGAGCGAATCGGTCATCCGTGAAGAAGGTGTTGAGCGTGCTCAGTATCTGATCGACCAACTGCTTGCTGaa < 1:404128/141‑1 (MQ=255) aGGAATAACCCATGTCAGAACGTTTCCCAAATGACGTGGATCCGTTCGAAACTCGCGACTGGCTCCCGGCGATCGAATCGGTCATCCGTGAAGAAGGTGTTGAGCGTGCTCAGTATCTGATCGACCAACTGCTTGATGAAGcc > 2:278978/1‑143 (MQ=255) ggAATAACCCATGTCAGAACGTTTCCCAAATGACGTGGATCCGTTCGAAACTCGCGACTGGCTCCAGGCGATCGAATCGGTCATCCGTGAAGAAGGTGTTGAGCGTGCTCAGTATCTGATCGACCAACTGCTTGCTGAAGccc < 2:896763/143‑1 (MQ=255) gtaTACCCCATGTCAGAACGTTGCCCAAGTGGCGTGGATCGGTTCGAAACTCGCGACTGGCTCCAGGCGATCGAATCGGTCATCCGTGAAGAAGGTGTTGAGCGTGCTCAGTATCTGATCGACCAACTGCTTGCTGAAGCCCg < 2:1185720/141‑1 (MQ=255) gAATAACCCATGTCAGAACGTTTCCCAAATGACGTGGATCCGTTCGAAACTCGCGACTGGCTCCAGGCGATCGACTCGGTCATCCGTGAAGAAGGTGTTGAGCGTGCTCAGTGTCTGATCGACCAACTGCTTGCTGAAGCCCg > 2:481083/1‑143 (MQ=255) aTAACCCATGTCAGAACGTTTCCCAAATGACGTGGATCCGATCGAAACTCGCGACTGGCTCCAGGCGATCGAATCGGTCATCCGTGAAGAAGGTGTTGAGCGTGCTCAGTATCTGATCGACCAACTGCTTGCTGAAGGCCGCa > 1:302823/1‑143 (MQ=255) tAACCCATGTCAGAACGTTTCCCAAATGACGTGGATCCGTTCGAAACTCGCGACTGGCTCCAGGCGATCGAAGCGGTCATCCGTGCAGAAGGTGTTGAGCGTGCTCAGTATCTGATCCCCCAACTGCCTGCTGACGCCCGCaa > 2:1147974/1‑143 (MQ=255) cccATGTCAGAACGTTGCCCAAATGACGTGGATCCGATCGAAACTCGCGACTGGCTCCAGGCGATCGAATCGGTCATCCGTGAAGAAGGTGTTGAGCGTGCTCAGTATCTGATCGACCAACTGCTTGCTGAAGCCCGCAAAgg < 1:393147/143‑1 (MQ=255) ccATGTCAGAACGTTTCCCAAATGACGTGGATCCGTTCGAAACTCGCGACTGGCTCCAGGCGATCGAATCGGTCATCCGTGAAGAAGGTGTTGAGCGTGCTCAGTATCTGATCGACCAACTGCTTGCTGAAGCCCGCAAAGGc > 1:84243/1‑143 (MQ=255) cATGTCAGACCGTTTCCCAAATGACGTGGATCCGTTCGAAACTCGCGACTGGCTCCAGGCGATCGAATCGGTCATCCGTGAAGAAGGTGTTGAGCGTGCTCAGTATCTGATCGACCAACTGCTTGCTGAAGCCCGCAAAGGCg < 2:968383/143‑1 (MQ=255) cATGTCAGAACGTTTCCCAAATGACGTGGATCCGTTCGAAACTCGCGACTGGCTCCAGGCGATCGACTCGGTCATCCGTGAAGAAGGTGTTGAGCATGCTCCGTATCTGATCGACCAACTGCTTGCTGAAGCCAGCATAGGCg > 2:674451/1‑143 (MQ=255) tgccAGACCGTTTCCCAAATGACGTGGATCCGTTCGAAACTCGCGACTGGCTCCAGGCGATCGAATCGGTCATCCGTGAAGAAGGTGTTGAGCGTGCTCAGTATCTGATCGACCAACTGCTTGCTGAAGCCCGCAAAGGCGgt < 2:333903/140‑1 (MQ=255) tGTCAGCACGTTTCCCAAATGACGTGGATCCGTCCGAAACTCGCGACTGGCTCCAGGCGATCGAATCGGTCATCCGTCAAGAAGGTGTTGAGCGTGCTCAGTATCTGATCGACCAACTGCTTGCTGAAGCCCGCAAAGGCGgt < 1:67277/143‑1 (MQ=255) aGAACGTTTCCCAAATGACGTGGATCCGATCGAAACTCGCGACTGGCTCCAGGCGATCGAATCGGTCATCCGTGAAGAAGGTGTTGAGCGTGCTCAGTATCTGATCGACCAACTGCTTGCTGAAGCCCGCAAAGGCGGTGGaa > 1:1059741/1‑143 (MQ=255) gAACGTTTCCCAAATGACATGGATCCGTCAGAAAGTAGTCATTTGCTCCAGGCGATCGAATCGGACATCGGAGAAAGAGACATAGAGCGTGCTCAGGATCTTGTCGACGAACTGGTTTCTGAAGCCCGCAAAAGCGGTGGaaa > 2:326020/1‑143 (MQ=255) aaCGTTTCCCAAATGACGTGGATCCGTTCGAAAGTCGCGACTGGCTCCAGGCGATCGAATCGGTCATCCGTGCAGAAGGTGCTGAGCGTGCTCAGTATCTGATCGACCAACTGCTTGCTGAAGCCCGCGCAGGCGgtgtccgc > 2:874953/1‑139 (MQ=255) tttCCCAAATGACGTGGATCCGTTCGAAACTCGCGACTGGCTCCAGGCGATCGAATCGGTCATCCGTGAAGAAGGTGTTGAGCGTGCTCAGTATCTGATCGACCAACTGCTTGCTGAAGCCCGCAAAGGCGGTGTAAACGTAg < 2:1215428/143‑1 (MQ=255) tttCCCAAATGACGTGGATCCGTTCGAAACTCGCGACTGGCTCCAGGCGATCGAATCGGTCATCCGTGAAGAAGGTGTTGAGCGTGCTCAGTATCTGATCGACCAACTGCTTGCTGAAGCCCGCAAAGGCGGTGTAAACGTAg > 1:1180808/1‑143 (MQ=255) ttCCCAAATGACGTGGATCCGATCGAAACTCGCGACTGGCTCCAGGCGATCGAATCGGTCATCCGTGAAGAAGGTGTTGAGCGTGCTCAGTATCTGATCGACCAACTGCTTGCTGAAGCCCGCAAAGGCGGTGTAAACGTAGc < 2:1160572/143‑1 (MQ=255) cccacggACGTGGAGCCGTTCGAAACTCGCGACTGGCTCCAGGCGATCGAATCGGTCATCCGTGAAGAAGGTGTTGAGCGTGCTCAGTATCTGATCGACCAACTGCTTGCTGAAGCCCGCAAAGGCGGTGTAAACGTAGCCGc < 2:476875/137‑1 (MQ=255) ccAAATGACGTGGATCCGTTCGAAACTCGCGACTGGCTCCAGGCGATCGAATCGGTCATCCGTGAAGAAGGTGTTGAGCGTGCTCAGTATCTGATCGACCAACTGCTTGCTGAAGCCCGCAAAGGCGGTGTAAACGTAGCCGc > 1:1224722/1‑143 (MQ=255) gactGACGTGGATCCGATCGAAACTCGCGACTGGCTCCAGGCGATCGAATCGGTCATCCGTGAAGAAGGTGTTGAGCGTGCTCAGTATCTGATCGACCAACTGCTTGCTGAAGCCCGCAAAGGCGGTGTAAACGTAGCCGCAg < 2:1129854/140‑1 (MQ=255) aaTGACGTGGATCCGTTCGAAACTCGCGACTGGCTCCAGGCGATCGAATCGGTCATCCGTGAAGAAGGTGTTGAGCGTGCTCAGTATCTGATCGACCAACTGCTTGCTGAAGCCCGCAAAGGCGGTGTAAACGTAGCCGCAgg < 1:1006800/143‑1 (MQ=255) tGGATCCGTTCGAAACTCGCGACTGGCTCCAGGCGATCGAATCGGTCATCCGTGAAGAAGGTGTTGAGCGTGCTCACTATCTGCTCGACCAACTGCTTGCTGAAGCCCGCAACGGCGGCGTCAACGTACCCGCAGGCACAGGt > 2:513745/1‑143 (MQ=255) tCCGTTCGAAACTCGCGACTGGCTCCAGGCGATCGAATCGGTCATCCGTGAAGAAGGTGTTGAGCGTGCTCAGTATCTGATCGACCAACTGCTTGCTGAAGCCCGCAAAGGCGGTGTAAACGTGGCGGCAGGCACAGGTACCa > 2:57589/1‑143 (MQ=255) tCCGTTCGAAACTCGCGACTGGCTCCAGGCGATCGAATCGGTCATCCGTGAAGAAGGTGTTGAGCGTGCTCAGTATCTGATCGACCAACTGCTTGCTGAAGCCCGCAAAGGCGGTGTAAACGTAGCCGCAGGCACAGGTATCa < 1:156083/143‑1 (MQ=255) tCCGTTCGAAACTCGCGACTGGCTCCAGGCGATCGAATCGGTCATCCGTGAAGAAGGTGTTGAGCGTGCTCAGTATCTGATCGACCAACTGCTTGCTGAAGCCCGCAAAGGCGGTGTAAACGTAGCCGCAGGCACAGGTATCa > 1:816134/1‑143 (MQ=255) | GCGGCAACTAAACGTAGAACCTGTCTTATTGAGCTTTCCGGCGAGAGTTCAATGGGACAGGTTCCAGAAAACTCAACGTTATTAGATAGATAAGGAATAACCCATGTCAGAACGTTTCCCAAATGACGTGGATCCGATCGAAACTCGCGACTGGCTCCAGGCGATCGAATCGGTCATCCGTGAAGAAGGTGTTGAGCGTGCTCAGTATCTGATCGACCAACTGCTTGCTGAAGCCCGCAAAGGCGGTGTAAACGTAGCCGCAGGCACAGGTATCA > NC_000913/122914‑123188 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |