Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	2,906,608	G→C	7.6%	intergenic (+25/+35)	ygcG → / ← eno	protein YgcG/enolase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,906,608	0	G	C	7.6%	192.2 / 5.7	68	intergenic (+25/+35)	ygcG/eno	protein YgcG/enolase
Reads supporting (aligned to +/- strand):  ref base G (26/35);  new base C (2/4);  total (28/40)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GCATCTGGTAGAAGTTATCATTCAGACAACAGCGGTTCCGCTGGAGGCTCAGATAGCGGCGGATTTAGCGGCGGGGGCGGTTCTTCTGGAGGTGGCGGCGCATCCGGCCGCTGGTAACTCAACCATAAAAAATGCCAGCC‑CGGAGGCTGGCATTTTTAAATCAGATAAAGTCAGTCTTATGCCTGGCCTTTGATCTCTTTACGACCGTTGTACGGTGCTTTTTCGCCCAGAGCTTCTTCGATACGAATCAGCTGGTTGTATTTAGCAACACGGTCAGAACG  >  NC_000913/2906467‑2906747                                                                                                                                               |                                                                                                                                            gCATCTGGTAGAAGTTATCATTCAGACAACAGCGGTTCCGCTGGAGGCTCAGATAGCGGCGGATTTAGCGGCGGGGGCGGTTCTTCTGGAGGTGGCGGCGCATCCGGCCGCTGGTAACTCAACCATAAAAAATGCCAGCC‑Cgg                                                                                                                                            <  2:142262/143‑1 (MQ=255)        gTAGAAGTTATCATTCAGACAACAGCGGTTCCGCTGGAGGCTCAGATAGCGGCGGATTTAGCGGCGGGGGCGGTTCTTCTGGAGGTGGCGGCGCATCCGGCCGCTGGTCACTCAACCATAAAAAATGCCAGCC‑CGGAGGCTgg                                                                                                                                     >  1:847122/1‑143 (MQ=255)        gTAGAAGTTATCATTCAGACAACAGCGGTTCCGCTGGAGGCTCAGATAGCGGCGGATTTAGCGGCGGGGGCGGTTCTTCTGGAGGTGGCGGCGCATCCGGCCGCTGGTAACTCAACCATAAAAAATGCCAGCC‑CGGAGGCTgg                                                                                                                                     <  1:357126/143‑1 (MQ=255)            aaGTTATCATTCAGACAACAGCGGTTCCGCTGGAGGCTCAGATAGCGGCGGATTTAGCGGCGGGGGCGGTTCTTCTGGAGGTGGCGGCGCATCCGGCCGCTGGTAACTCAACCATAAAAAATGCCAGCC‑CGGAGGCTGGCAtc                                                                                                                                 >  2:1048686/1‑142 (MQ=255)             aGTTATCATTCAGACACCAGCGGTTCCGCTGGAGGCTCAGATAGCGGCGGATTTAGCGGCGGGGGCGGTTCTTCTGGAGGTGGCGGCGCATCCGGCCGCTGGTAACTCAACCATAAAAAATGCCAGCCTCCG‑GGCTGGCAttt                                                                                                                                <  1:372634/143‑1 (MQ=255)              gtgaTCATTCCGACCTCAGCGGTTCCGCTGGAGGCTCAGATAGCGGCGGATTTCGCGGCGGGGGCGGTTCTTCTGGAGGTGGCGGCGCATCCGGCCGCTGGTAACTCAACCATAAAAAATGCCAGCC‑CGGAGGCTGGCAtttt                                                                                                                               <  2:652768/140‑1 (MQ=255)              gTTATCATGCAGACCACGGCGGTTCCGCTGGAGGCTCAGATAGCGGCGGATTTAGCGGCGGGGGCGGTTCTTCTGGAGGTGGCGGCGCATCCGGCCGCTGGTAACTCAACCATAAAAAATGCCAGCC‑TCCGGGCTGGCAtttt                                                                                                                               <  2:644097/143‑1 (MQ=255)                 aTCATTCAGACAACAGCGGTTCCGCTGGAGGCTCAGATAGCGGCGGATTTAGCGGCGGGGGCGGTTCTTCTGGAGGTGGCGGCGCATCCGGCCGCTTGTAACTCAACCATACAACATGCCAGCC‑TCCGGGCTGGCAtttttta                                                                                                                            <  1:810191/143‑3 (MQ=255)                  tCATTCAGACAACAGCGGTTCCGCTGGAGGCTCAGATAGCGGCGGATTTAGCGGCGAGCGCGGCTCTTTTGGAGCCCCCCGCGCATCCGGCCGCTGGTCACTCAACCATAAAAAATGCCAGCC‑CGGAGGCTGGCATTTTTaaa                                                                                                                           >  1:594921/1‑143 (MQ=255)                    aTTCAGACAACAGCGGTTCCGCTGGAGGCTCAGATAGCGGCGGATTTAGCGGCGGGGGCGGTTCTTCTGGAGGTGGCGGCGCATCCGGCCGCTGGTAACTCAACCATAAAAAATGCCAGCC‑CGGAGGCTGGCATTTTTAAATc                                                                                                                         <  1:1008223/143‑1 (MQ=255)                     ttCAGACAACAGCGGTTCCGCTGGAGGCTCAGATAGCGGCGGATTTAGCGGCGGGGGCGGTTCTTCTGGAGGTGGCGGCGCATCCGGCCGCTGGTAACTCAACCATAAAAAATGCCAGCC‑CGGAGGCTGGCATTTTTAAATCa                                                                                                                        <  2:40461/143‑1 (MQ=255)                     ttCAGACAACAGCGGTTCCGCTGGAGGCTCAGATAGCGGCGGATTTAGCGGCGGGGGCGGTTCTTCTGGAGGTGGCGGCGCATCCGGCCGCTGGTAACTCAACCATAAAAAATGCCAGCC‑CGGAGGCTGGCATTTTTAAATCa                                                                                                                        <  1:159671/143‑1 (MQ=255)                      tCAGACAACCGCGGTTCCGCTGGAGGCTCAGCTAGCGGCGGATTTAGCGGCGGGGGCGGTTCTTCTGGAGGTGGCGGCGCATCCGGCCGCTGGTAACTCAACCATAAAAAATGCCAGCC‑CGGAGGCTGGCATTTTTAAATCAg                                                                                                                       <  1:565460/143‑1 (MQ=255)                        aGACAACAGCGGTTCCGCTGGAGGCTCAGATAGCGGCGGATTTAGCGGCGGGGGCGGTTCTTCTGGAGGTGGCGGCGCATCCGGCCGCTGGTAACTCAACCATAAAAAATGCCAGCC‑CGGAGGCTGGCCTTTTTAAATCAGAt                                                                                                                     >  1:335248/1‑143 (MQ=255)                        aGACAACAGCGGTTCCGCTGGAGGCTCAGATAGCGGCGGATTTAGCGGCGGGGGCGGTTCTTCTGGAGGTGGCGGCGCATCCGGCCGCTGGTAACTCAACCATAAAAAATGCCAGCC‑CGGAGGCTGGCATTTTTAAATCAGAt                                                                                                                     >  1:86605/1‑143 (MQ=255)                            aacaGCGGTTCCGCTGGAGGCTCAGATAGCGGCGGATTTAGCGGCGGGGGCGGTTCTTCTGGAGGTGGCGGCGCATCCGGCCGCTGGTAACTCAACCATAAAAAATGCCAGCC‑CGGAGGCTGGCATTTTTAAATCAGATAAag                                                                                                                 >  1:98291/1‑143 (MQ=255)                            aacaGCGGTTCCGCTGGAGGCTCAGATAGCGGCGGATTTAGCGGCGGGGGCGGGTCTTCTGGAGGTGGCGGCGCATCCGGCCGCTGGTAACTCAACCATAAAAAATGCCGGCC‑ACGGGGCTGGCCGTTTTAAATCAGATAAag                                                                                                                 >  1:161564/1‑143 (MQ=255)                             acaGCGGTTCCGCTGGAGGCTCAGATAGCGGCGGATTTAGCGGCGGGGGCGGTTCTTCTGGAGGTGGCGGCGCATCCGGCCGCTGGTAACTCAACCATAAAAAATGCCAGCC‑CGGAGGCTGGCATTTTTAAATCAGATAAagt                                                                                                                <  2:358184/143‑1 (MQ=255)                                            gAGGCTCAGATAGCGGCGGATTTAGCGGCGGGGGCGGTTCTTCTGGAGGTGGCGGCGCATCCGGCCGCTGGTAACTCAACCATAAAAAATGCCAGCC‑CGGAGGCTGGCATTTTTAAATCAGATAAAGTCAGTCTTATGCCTgg                                                                                                 <  2:875168/143‑1 (MQ=255)                                                 tCAGATAGCGGCGGATTTAGCGGCGGGGGCGGTTCTTCTGGAGGTGGCGGCGCATCCGGCCGCTGGTAACTCAACCATAAAAAATGCCAGCC‑CGGAGGCTGGCATTTTTAAATCAGATAAAGTCAGTCTTATGCCTGGCCttt                                                                                            <  1:19291/143‑1 (MQ=255)                                                   aGATCGCGGCGGATTTAGCGGCGGGGGCGGTTCTTCTGGAGGTGGCGGCGCATCCGGCCGCTGGTAACTCAACCATAAAAAATGCCAGCC‑CGGAGGCTGGCATTTTTAAATCAGATAAAGTCAGTCTTATGCCTGGCCTTTGa                                                                                          <  2:355506/143‑1 (MQ=255)                                                   aGATAGCGGCGGATTTAGCGGCGGGGGCGGTTCTTCTGGAGGTGGCGGCGCATCCGGCCGCTGGTAACTCAACCATAAAAAATGCCAGCC‑CGGAGGCTGGCATTTTTAAATCAGATAAAGTCAGTCTTATGCCTGGCCTTTGa                                                                                          <  1:1181730/143‑1 (MQ=255)                                                    gATGGCGGCGGATTTAGCGGCGGGGGCGGTTCTTCTGGAGGTGGCGGCGCATCCGGCCGCTGGTAACTCAACCATAAAAAATGCCAGCC‑CGGAGGCTGGCATTTTTAAATCAGATAAAGTCAGTCTTATGCCTGGCCTTTGAt                                                                                         <  2:114318/143‑1 (MQ=255)                                                    gATAGCGGCGGATTTAGCGGCGGGGGCGGTTCTCCTGGAGGTGGCGGCGCATCCGGCCGCTGGTAACTCAACCATAAAAAATGCCAGCC‑CGGAGGCTGGCATTTTTAAATCAGATAAAGTCAGTCTTATGCCTGGCCTTTGAt                                                                                         <  1:1009274/143‑1 (MQ=255)                                                    gATAGCGGCGGATTTAGCCGCGGGGGCGGCGCGTCTGGAGGTGGCGGCGCATCCGGCCGCTGGTAACTCAACCATAAAAAATGCCAGCC‑CGGAGGCTGGCATTTTTAAATCAGATAAAGTCAGTCTTATGCCTGGCCTTTGAt                                                                                         <  2:614025/143‑1 (MQ=255)                                                        gcggcgGATTTAGCGGCGGGGGCGGTTCTTCTGGAGGTGGCGGCGCATCCGGCCGCTGGTAACTCAACCATAAAAAATGCCAGCC‑CGGAGGCTGGCATTTTTAAATCAGATAAAGTCAGTCTTATGCCTGGCCTTTGATctct                                                                                     <  1:1188612/143‑1 (MQ=255)                                                          ggcggATTTAGCGGCGGGGGCGGTTCTTCTGGAGGTGGCGGCGCATCCGGCCGCTGGTAACTCAACCATAAAAAATGCCAGCC‑CGGAGGCTGGCATTTTTAAATCAGATAAAGTCAGTCTTATGCCTGGCCTTTGATCTCttt                                                                                   >  1:203981/1‑143 (MQ=255)                                                            cggATTTAGCGGCGGGGGCGGTTCTTCTGGCGGTGGCGGCGCATCCCGCCGCTTGTAACTCAACCATAAAAAATGCCAGCC‑CGGAGGCTGGCATTTTTAAATCAGATAAAGTCAGTCTTATGCCTGGCCTTTGATCTCTTTAc                                                                                 <  2:445061/143‑1 (MQ=255)                                                            cggATTTAGCGGCGGGGGCGGTTCTTCTGGAGGTGGCGGCGCATCCGGCCGCTGGTAACTCAACCATAAAAAATGCCAGCC‑CGGAGGCTGGCATTTTTAAATCAGATAAAGTCAGTCTTATGCCTGACCTTTGATCTCTTTAc                                                                                 <  1:822200/143‑1 (MQ=255)                                                                tttAGCGGCGGGGGCGGTTCTTCTGGAGGTGGCGGCGCATCCGGCCGCTGGTAACTCAACCATAAAAAATGCCAGCC‑CGGAGGCTGGCATTTTTAAATCAGATAAAGTCAGTCTTATGCCTGGCCTTTGATCTCCTTACGAcc                                                                             >  2:726016/1‑143 (MQ=255)                                                                   aGCGGCGGGGGCGGTTCTTCTGGAGGTGGCGGCGCATCCGGCCGCTGGTAACTCAAGCATAAAAAATGCCAGCC‑CGGAGGCTGGCCTTTTTAAATCAGATAAAGTCAGTCTTATGCCTGGCCTTTGATCTCCTTACGACCGtt                                                                          >  2:1266345/1‑143 (MQ=255)                                                                      ggcggGGGCGGTTCTTCTGGAGGTGGCGGCGCATCCGGCCGCTGGTAACTCAACCATAAAAAATGCCAGCC‑CGGAGGCTGGCATTTTTAAATCAGATAAAGTCAGTCTTATGCCTGGCCTTTGATCTCTTTACGACCGTTGTa                                                                       >  1:714143/1‑143 (MQ=255)                                                                       gcggGGGCGGTTCTTCTGGAGGTGGCGGCGCGTCCGGCCGCTGGTAACTCAACCATAAAAAATGCCAGCC‑CGGAGGCTGGCATTTTTAAATCAGATAAAGTCAGTCTTATGCCTGGCCTTTGATCTCTTTACGACCGTTGTAc                                                                      <  2:1091531/143‑1 (MQ=255)                                                                               ggTTCTTCTGGAGGTGGCGGCGCATCCGGCCGCTGGTAACTCAACCATAAAAAATGCCAGCC‑CGGAGGCTGGCATTTTTAAATCAGGTAAAGTCAGTCTTATGCCTGGCCCTTGATCTCTTTACGACGTTTGTCCgggcgtct                                                              >  2:108985/1‑137 (MQ=255)                                                                               ggTTCTTCTGGAGGTGGCGGCGCATCCGGCCGCTGGTAACTCAACCATAAAAAATGCCAGCC‑CGGAGGCTGGCATTTTTAAATCAGATAAAGTCAGTCTTATGCCTGGCCTTTGATCTCTTTACGACCGTTGTACGGTGCttt                                                              >  2:1260120/1‑143 (MQ=255)                                                                               ggTTCTTCTGGAGGTGGCGGCGCATCCGGCCGCTGGTAACTCAACCATAAAAAATGCCAGCC‑CGGAGGCCGGCATTTTTAAATCAGATAAAGTCAGTCTTATGCCCGGCCTTTGATCTCTATAGGACCATTCTACGGAGcctc                                                              >  2:429560/1‑140 (MQ=255)                                                                               ggTTCTTCGGGAGGTGGCGGCGCATCCGGCCGCTGGTAACTCAACCATAAAAAATGCCAGCC‑CGGAGGCTGGCATTTTTAAATCAGATAAAGTCAGTCTTATGCCTGGCCTTTGATCTCTTTACGACCGTTGTACGGTGCttt                                                              <  1:92591/143‑1 (MQ=255)                                                                                  tcttctGGAGGTGGCGGCGCATCCGGCCGCTGGTAACTCAACCATAAAAAATGCCAGCC‑CGGAGGCTGGCATTTTTAAATCAGATAAAGTCAGTCTTATGCCTGGCCTTTGATCTCTTTACGACCGTTGTACGGTGCTTTTTc                                                           >  1:740917/1‑143 (MQ=255)                                                                                  tcttctGGAGGTGGCGGCGCATCCGGCCGCTGGTAACTCAACCATAAAAAATGCCAGCC‑CGGAGGCTGGCATTTTTAAATCAGATAAAGTCAGTCTTATGCCTGGCCTTTGATCTCTTTACGACCGTTGTACGGTGCTTTTTc                                                           >  1:393512/1‑143 (MQ=255)                                                                                    ttctGGAGGTGGCGGCGCATCCGGCCGCTGGTAACTCAACCATAAAAAATGCCAGCC‑CGGAGGCTGGCATTTTTAAATCAGATAAAGTCAGTCTTATGCCTGGCCTTTGATCTCTTTACGACCGTTGTACGGTGCTTTTTCGc                                                         >  2:60964/1‑143 (MQ=255)                                                                                     tctGGAGGTGGCGGCGCATCCGGCCGCTGGTAACTCAACCATAAAAAATGCCAGCC‑CGGAGGCTGGCATTTTTAAATCAGATAAAGTCAGTCTTATGCCTGGCCTTTGATCTCTTTACGACCGTTGTACGGTGCTTTTTCGcc                                                        >  2:477650/1‑143 (MQ=255)                                                                                       tGGAGGTGGCGGCGCACCCGGCCGCTGGTAACTCAACCATAAAAAAGGCCAGCC‑CGGAGGCTGGCATTGTTAAATCAGATAAAGTCAGTCTTATGCCTGGCCTTTGATCTCTTTACGACCGTTGTACGGTGCTTTTTCGCCCa                                                      <  1:28264/143‑1 (MQ=255)                                                                                          aGGTGGCGGCGCATCCGGCCGCTGGTAACTCAACCATAAAAAATGCCAGCC‑CGGAGGCTGGCATTTTTAAATCAGATAAAGTCAGTCTTATGCCTGGCCTTTGATCTCTTTACGACCGTTGTACGGTGCTTTTTCGCCCagag                                                   <  1:499625/143‑1 (MQ=255)                                                                                             tGGCGGCGCATCCGGCCGCTGGTAACTCAACCATAAAAAATGCCAGCC‑CGGAGGCTGGCATTTTTAAATCAGATAAAGTCAGTCTTATGCCTGGCCTTTGATCTCTTTACGACCGTTGTACGGTGCTTTTTCGCCCAGAGctt                                                >  1:640351/1‑143 (MQ=255)                                                                                                cggcgCATCCGGCCGCTGGTAACTCAACCATAAAAAATGCCAGCC‑CGGAGGCTGGCATTTTTAAATCAGATAAAGTCAGTCTTATGCCTGGCCTTTGATCCCTTTACGACCGTTGTACGGTGCCTTTTCGCCCCGCGcttctt                                             >  2:949045/1‑143 (MQ=255)                                                                                                 ggcgCATCCGGCCGCTGGTAACTCAACCATAAAAAATGCCAGCC‑CGGAGGCTGGCATTTTTAAATCAGATAGAGTCAGTCTTATGCCTGGCCTTTGATCTCTTTACGACCGTTGTACGGTGCTTTTTCGCCCAGAGCttcttc                                            <  2:1114160/143‑1 (MQ=255)                                                                                                     ccttgggCCGCTGGTAAGTCAATCATAATAAATGGCAGCC‑CGGAGGCTGGCATTTTTAAATCAGATAAAGGCAGTCTTATGCCTGGCCTTTGATCTCTTTACGACCGTTGTACGGTGCTTGTTCGCCCAGAGCTTCTTCGATa                                        <  2:809038/138‑1 (MQ=255)                                                                                                           gCCGCTGGTAACTCAACCATAAAAAATGCCAGCC‑CGGAGGCTGGCATTTTTAAATCAGATAAAGTCAGTCTTATGCCTGGCCTTTGATCTCTTTACGACCGTTGTACGGTGCTTTTTCGCCCAGCGCTTATTCGATACGAATc                                  >  2:583548/1‑143 (MQ=255)                                                                                                             cGCTGGTAACTCAACCATAAAAAATGCCAGCC‑CGGAGGCTGGCATTTTTAAATCAGATAAAGTCAGTCTTATGCCTGGCCTTTGATCTCTTTACGACCGTTGTACGGTGCTTTTTCGCCCAGAGCTTCTTCGATACGAATCAg                                <  1:1217486/143‑1 (MQ=255)                                                                                                                    accTCAACCATAAAAAATGCCAGCC‑CGGAGGCTGGCATTTTTAAATCAGATAAAGTCAGTCTTATGCCTGGCCTTTGATCTCTTTACGACCGTTGTACGGTGCTTTTTCGCCCAGAGCTTCTTCGATACGAATCAGCTGGTTg                         <  2:751174/141‑1 (MQ=255)                                                                                                                     aCTCAACCATAAAAAATGCCAGCC‑CGGAGGCTGGCATTTTTAAATCAGATAAAGTCAGTCTTATGCCTGGCCTTTGATCTCTTTACGACCGTTGTACGGTGCTTTTTCGCCCAGAGCTTCTTCGATACGAATCAGCTGGTTGt                        <  2:86605/143‑1 (MQ=255)                                                                                                                     aCTCAACCATAAAAAATGCCAGCC‑CGGAGCCTGGCATTTTTAAATCAGATAAAGTCAGTCTTATGCCTGGCCTTTGATCTCTTTACGACCGTTGTACGGTGCTTTTTCGCCCAGAGCTTCTTCGATACGAATCAGCTGGTTGt                        <  1:70403/143‑1 (MQ=255)                                                                                                                      cTCAACCATAAAAAATGCCAGCC‑CGGAGGCTGGCATTTTTAAATCAGATAAAGTCAGTCTTATGCCTGGCCTTTGATCTCTTTACGACCGTTGTACGGTGCTTTTTCGCCCAGAGCTTCTTCGATACGAATCAGCTGGTTGTa                       >  1:1279078/1‑143 (MQ=255)                                                                                                                         ctgatttaaaaaTGCCAGCCTCCG‑GGCTGGCATTTTTAAATCAGATAAAGTCAGTCTTATGCCTGGCCTTTGATCTCTTTACGACCGTTGTACGGTGCTTTTTCGCCCAGAGCTTCTTCGATACGAATCAGCTGGTTGTAttt                    >  1:56460/8‑143 (MQ=38)                                                                                                                         aaCCATAAAAAATGCCAGCC‑CGGAGGCTGGCATTTTTAAATCAGATAAAGTCAGTCTTATGCCTGGCCTTTGATCTCTTTACGACCGTTGTACGGTGCTTTTTCGCCCAGAGCTTCTTCGATACGAATCAGCTGGTTGTAttt                    <  2:594921/143‑1 (MQ=255)                                                                                                                         aaCCATAAAAAATGCCAGCC‑CGGAGGCTGGCATTTTTAAATCAGATAAAGTCAGTCTTATGCCTGGCCTTTGATCTCTTTACGACCGTTGTACGGTGCTTTTTCGCCCAGAGCTTCTTCGATACGAATCAGCTGGTTGTAttt                    <  2:1279447/143‑1 (MQ=255)                                                                                                                         aaCCATAAAAAATGCCAGCC‑CGGAGGCTGGCATTTTTAAATCAGATAAAGTCAGTCTTATGCCTGGCCTTTCATCTCTTTACGACCGTTGTACCGTGCTTTTTCGCCCAGAGCTTCTTCTATTCGAATTAGCTTGtttttttt                    >  1:48772/1‑143 (MQ=255)                                                                                                                          aCCATACAAAATGCCAGCC‑CGGAGGCTGGCATTTTGAAATCAGATAAAGTCAGTCTTATGCCTGGCCTTTGATCTCTTTACGACCGTTGTACGGTGCTTTTTCGCCCAGAGCTTCTTCGATACGAATCAGCTGGTTGTATTTa                   <  2:863995/143‑1 (MQ=255)                                                                                                                              tAAAGACTGCCAGCC‑CGGAGGCTGGCATTTTTAAATCAGATAAAGTCAGTCTTATGCCTGGCCTTTGATCTCTTTACGACCGTTGTACGGTGCTTTTTCGCCCAGAGCTTCTTCGATACGAATCAGCTGGTTGTATTTAGCaa               <  2:325325/143‑1 (MQ=255)                                                                                                                              tAAAAAATGCCAGCC‑CGGAGGCTGGCATTTTTAAATCAGATAAAGTCAGTCTTATGCCTGGCCTTTGATCTCTTTACGACCGTTGTACGGTGCTTTTTCGCCCAGAGCTTCTTCGATACGAATCAGCTGGTTGTATTTAGCaa               <  1:378973/143‑1 (MQ=255)                                                                                                                                    ctGCCAGGT‑CGGTGGGTGGCATTGTCAAATTCCCTAATTTCAGTCTGATGCCTGGCCTTTGATCTCTTTACGCCCGTTGTACGGTGCTTTTTCGCCCAGAGCTTCTTCGATACGAATCAGCTGGTTGTATTTAGCAACACGGt         <  2:560403/142‑1 (MQ=255)                                                                                                                                    aTGCCAGCC‑CGGAGGCTGGCATTTTTAAATCAGATAAAGTCAGTCTTATGCCTGGCCTTTGATCTCTTTACGACCGTTGTACGGTGCTTTTTCGCCCAGAGCTTCTTCGATACGAATCAGCTGGTTGTATTTAGCAACACGGt         <  1:764546/143‑1 (MQ=255)                                                                                                                                    aTGCCAGCC‑CCGAGGCTGGCATTTTTAAATCAGATAACGTCAGTCTTATGCCTGGCCTTTGATCTCTTTACGACCGTTGTACGGTGCTTTTTCGCCCAGAGCTTCTTCGATACGAATCAGCTGGTTGTATTTAGCAACACGGt         <  1:603140/143‑1 (MQ=255)                                                                                                                                     tGCCAGCC‑CGGAGGCTGGCATTTTTAAATCAGATAAAGTCAGTCTTATGCCTGGCCTTTGATCTCTTTACGACCGTTGTACGGTGCTTTTTCGCCCAGAGCTTCTTCGATACGAATCAGCTGGTTGTATTTAGCAACACGGTc        <  2:203981/143‑1 (MQ=255)                                                                                                                                       ccAGGC‑CTGAGGCTGGCATTTTTAAATCAGATAAAGTCAGTCTTATGCCTGGCCTTTGATCTCTTTTAGGCCGTTGTACGGTGCTTTTTCGCCCAGAGCTTCTTCGATACGAATCAGCTGGTTGTATTTAGCAACACGGTCAg      <  2:773119/143‑1 (MQ=255)                                                                                                                                       ccAGCC‑CGGAGGCTGGCATTTTTAAATCAGATAAAGTCAGTCTTATGCCTGGCCTTTGATCTCTTTACGACCGTTGTACGGTGCTTTTTCGCCCAGAGCTTCTTCGATACGCACCAGCTGATTGTGTTTAGCAAAATGGTCAg      >  2:1084542/1‑143 (MQ=255)                                                                                                                                       ccAGCC‑CGGAGGCTGGCATTTTTAAATCAGATAAAGTCAGTCTTATGCCTGGCCTTTGATCTCTTTACGACCGTTGTACGGTGCTTTTTCGCCCAGAGCTTCTTCGATACGAATCAGCTGGTTGTATTTAGCAAAACGGTCAg      >  1:240948/1‑143 (MQ=255)                                                                                                                                         aGCC‑CGGAGGCTGGCATTTTTAAATCAGATAAAGTCAGTCTTATGCCTGGCCTTTGATCTCTTTACGACCGTTGTACGGTGCTTTTTCGCCCAGAGCTTCTTCGATACGAATCAGCTGGTTGTATGTAGCCAGCCGGTCAGaa    >  2:1155489/1‑143 (MQ=255)                                                                                                                                           cc‑cGGAGGCTGGCATTTTTAAATCAGATAAAGTCAGTCTTATGCCTGGCCTTTGATCTCTTTACGACCGTTATACGGTGCTTTTTCGCCCAGAACTTCTTCGATACGAATCAGCTGGTTGTATTTAGCAACACTTTCAAAACg  >  1:764481/1‑143 (MQ=255)                                                                                                                                               |                                                                                                                                            GCATCTGGTAGAAGTTATCATTCAGACAACAGCGGTTCCGCTGGAGGCTCAGATAGCGGCGGATTTAGCGGCGGGGGCGGTTCTTCTGGAGGTGGCGGCGCATCCGGCCGCTGGTAACTCAACCATAAAAAATGCCAGCC‑CGGAGGCTGGCATTTTTAAATCAGATAAAGTCAGTCTTATGCCTGGCCTTTGATCTCTTTACGACCGTTGTACGGTGCTTTTTCGCCCAGAGCTTCTTCGATACGAATCAGCTGGTTGTATTTAGCAACACGGTCAGAACG  >  NC_000913/2906467‑2906747

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑