| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_000913 | 3,041,398 | T→G | 100% | L29R (CTT→CGT) | ygfZ → | folate‑binding protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 3,041,398 | 0 | T | G | 100.0% | 163.8 / ‑6.7 | 57 | L29R (CTT→CGT) | ygfZ | folate‑binding protein |
| Reads supporting (aligned to +/- strand): ref base T (0/1); new base G (25/30); total (26/31) | |||||||||||
| Rejected as polymorphism: E-value score below prediction cutoff. | |||||||||||
| Rejected as polymorphism: Frequency below/above cutoff threshold. | |||||||||||
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. | |||||||||||
AGCTCTTTTACCATTAGTCATTAATACGCCGTTAAGCAACTCAGGACTCTATTATGGCTTTTACACCTTTTCCTCCCCGTCAGCCTACGGCTTCTGCCCGTTTACCGCTGACGCTGATGACGCTTGATGACTGGGCGCTTGCCACCATTACTGGCGCGGACAGCGAAAAATATATGCAGGGTCAGGTGACAGCAGATGTCAGCCAGATGGCAGAAGATCAGCACCTGCTCGCCGCCCATTGCGACGCCAAAGGTAAAATGTGGAGCAATTTACGT > NC_000913/3041260‑3041534 | aGCTCTTTTACCAGTAGTCGGTGATGCGCCGTTAAGCAACTCAGGACTCTATTGTGGCTTTTACACCGTTTCCGCCCCGGCAGCCTACGGCGTCTGCCCGTTTACCGCTGACGCTGATGACGCTTGATGACTGGGCGCGTGcc < 2:37057/143‑1 (MQ=255) ctctTTTACCATTAGTCATTAATACGCCGTTAAGCAACTCAGGACTCTATTATGGCTTTTACACCTTTTCCTCCCCGTCAGCCTACGGCTTCTGCCCGGTTACCGCTGACGCTGATGACGCTTGAGGCCTGGGCGCGTGccac > 1:970413/1‑143 (MQ=255) tACCATTAGTCAGTAATACGCCGTTAAGCAACTCAGGACTCTATTATGGCTTTTACACCTTTTCCTCCCCGTCAGCCTACGGCTTCTGCCCGTTTACCGCTGACGCTGATGACGCTTGATGACTGGGCGCGTGCCACCATTAc < 2:356828/143‑1 (MQ=255) ttAGTCATTAATACGCCGTTAAGCAACTCAGGACTCTATTATGGCTTTTACACCTTTTCCTCCCCGTCAGCCTACGGCTTCTGCCCCTTTCCCGCTGTCGATTATGTCGCTTGATGCCCGGGCCCGTGCCACCCTTatcgtgg > 2:882542/1‑137 (MQ=255) tCATTAATACGCCGTTAAGCAACTCAGGACTCTATTATGGCTTTTACACCTTTTCCTCCCCGTCAGCCTACGGCTTCTGCCCGTTTACCGCTGACGCTGATGACGCTTGATGACTGGGCGCGTGCCACCATTACTGGCGCggc > 1:610265/1‑142 (MQ=255) tCATTAATACGCCGTTAAGCAACTCAGGACTCTATTATGGCTTTTACACCTTTTCCTCCCCGTCAGCCTACGGCTTCTGCCCGTTTACCCCTGACGCTGATGGCGCTTGATCACTGCGCGCGTGCCCCCATCGCTGGCGCggc > 2:142591/1‑142 (MQ=255) aTTAATACGCCGTTAAGCAACTCAGGACTCTATTATGGCTTTTACACCTTTTCCTCCCCGTCAGCCTACGGCTTCTGCCCGTTTACCGCTGACGCTGATGACGCTTGATGCCTGGGCGCGTGCCACCCTTCCTGGCGCgggcg > 1:921816/1‑140 (MQ=255) aTTAATACGCCGTTAAGCAACTCAGGACTCTATTATGGCTTTTACACCTTTTCCTCCCCGTCAGCCTACGGCTTCTGCCCGTTTACCCCTGACGCTGATGACGCTTGATGACTGGGAGCGTGCAACAAATACTAGCGCGGACa > 1:218804/1‑143 (MQ=255) ccaacaCGCCGTTAAGCAACTCAGGACTCTATTACGGCTTTTACACCTTTTCCTCCCCGTCAGCCTACGGCTTCTGCCCGTTTACCGCTGACGCTGATGACGCTTGATGACTGGGCGCGTGCCACCATTACTGGCGCGGACAg < 1:347589/138‑1 (MQ=255) gaaTACGCCGTTAAGCAACTCAGGACTCTATTATGGCTTTTACACCTTTTCCTCCCCGTCAGCCTACGGCTTCTGCCCGTTTACCGCTGACGCTGATGACGCTTGATGACTGGGCGCGTGCCACCATTACTGGCGCGGACAGc < 1:906194/142‑1 (MQ=255) aTACGCCGTTAAGCAACTCAGGACTCTATTATGGCTTTTACACCTTTTCCTCCCCGTCAGCCTACGGCTTCTGCCCGTTTACCGCTGACGCTGATGACGCTTGATGACTGGGCGCGTGCCACCATTACTGGCGCGGACGGCGa > 2:179453/1‑143 (MQ=255) aCGCCGTTAAGCAACTCAGGACTCTATTATGGCTTTTACACCTTGTCCTCCCCGTCAGCCTACGGCTTCTGCCAGTATCACGCGGACGCTGATGACGCTTGATGACTGGGCGCCTGACACCAGTACTGGCCCGGACacagcag > 2:448050/1‑137 (MQ=255) gCAACTCAGGACTCTATTATGGCTTTTACACCTTTTCCTCCCCGTCAGCCTACGGCTTCTGCCCGCTTACCGCTGACGCTGATGACGCTTGATGACTGCGCGCGTGCCACCATTACTGGCGCGGACAGCGAAAAATATATGCa > 2:766383/1‑143 (MQ=255) tggctctATTATGGCTTTTGCGCCTTTTCCGCCCCGTCAGCCTACGGCTTCTGCCCGTTTACCGCTGACGCTGAGGACGCTTGATGACTGGGCGCGTGCCACCATTACTGGCGCGGACAGCGAAAAATATATGCAGGGTCAgg < 2:525944/140‑1 (MQ=255) ggACTCTATTATGGCTTCTACTCCTTTTCCTCCCCGTCAGCCTACGGCTTCTGCCCGTTTACCGCTGACGCTGATGACGCTTGATGACTGGGCGCGTGCCACCATTACTGGCGCGGACAGCGAAAAATATATGCAGGGTCAgg < 2:31020/143‑1 (MQ=255) ttattcgggCTTTTCCACCTTTTCCTCGCCGTCAGCCTACGGCTTCTGCCCGTTGACCGCGGACGCTGATGGCGCTTGATGACTGGGCGCGTGCCACCATTACTGGCGCGGAGACCGAAAAATATATGCAGGGTCAGGTGAca < 2:457274/136‑1 (MQ=255) tatgCTTTTACACCTTTTCCTCCCCGTCAGCCTACGGCTTCTGCCCGTTTACCGCTGACGCTGATGACGCTTGATGACTGGGCGCGTGCCACCATTACTGGCGCGGACAGCGAAAAATATATGCAGGGTCAGGTGACAGCAGa < 1:183251/140‑1 (MQ=255) atGGCTTTTACACCTTTTCCTCCCCGTCAGCCTACGGCTTCTGCCCGTTTACCGCTGACGCTGATGACGCTTGATGACTGGGCGCGTGCCGCCATTACTGGCGCGGACAGCGAAAAATATATGCAGGGTCAGGTGACAGCAGa > 1:335841/1‑143 (MQ=255) cTTTTACACCTTTTCCTCCCCGTCAGCCTACGGCTTCTGCCCGTTTACCGCTGACGCTGATGACGCTTGATGACTGGGCGCGTGCCACCATTACTGGCGCGGACAGCGAAAAATATATGCAGGGTCAGGTGACAGCAGATGTc > 2:15535/1‑143 (MQ=255) cTTTTACACCTTTTCCTCCCCGTCAGCCTACGGCTTCTGCACGTTTACCGCTGACGCTGATGACGCTTGATGACTGGGCGCGTGCCACCATTACTTGCGCGGGACCCGAAAGAAAGATGAAGGGTCGGGGGAcagcagggggc > 1:531219/1‑138 (MQ=255) tgttACCCCTTTGCCTCCCCGTCAGCCTACGGCTTCTGCCCGTTTACCGCTGACGCTGATGACGCTTGATGACTGGGCGCGTGCCACCATTACTGGCGCGGACAGCGAAAAATATATGCAGGGTCAGGTGACAGCAGATGTCa < 1:615340/141‑1 (MQ=255) tttCCTCCCCGTCAGCCTACGGCTTCTGCCCGTTTACCGCTGACGCTGATGACGCTTGATGACTGGGCGCGTGCCACCATTACTGGCGCGGACAGCGAAAAATATATGCAGGGTGAGGCGCAAGCAGATGTCAGCCAGATGGc > 2:201302/1‑143 (MQ=255) ttCCTCCCCGTCGCGTTCCGGCTTCGCGCCGGCTACCGCTGACGCTGGTGACGCTGGACGACTCGGCGCTTGCCACCTTTACTCGCGCGGACAGCGAAAAATATATGCAGGGTCAGGTGACAGCAGATGTCAGCCAGATGGCa < 2:422608/143‑1 (MQ=255) cctccCCGTCAGCCTACGGCTTCTGCCCGTTTACCGCTGACGCTGATGACGCTTGATGACTGGGCGCGTGCCACCATTACTGGCGCGGACAGCGAAAAATATATGCAGGGTCAGGTGACAGCAGATGTCAGCCAGATGGCaga < 1:1158474/143‑1 (MQ=255) ctctCCCTCAGCCTCCGGCTTCTTCCCGTTTACCGCTTACGCTGATGACGCTTGATGACTGGGCGCGTGCCACCATTACTGGCGCGGACAGCGAAAAATATCTGCAGGGTCAGGTGACAGCAGATGTCAGCCAGATGGCagaa < 2:804125/143‑1 (MQ=255) ctccCCGTCAGCCTACGGCTTCTGCCCGTTTACCGCTGACGCTGATGACGCTTGATGACTGGGCGCGTGCCACCATTACTGGCGCGGACAGCGAAAAGAATATGCAGGGTGAGGTGACAGCAGATGGCAGCCAGATGGCagaa > 2:1078362/1‑143 (MQ=255) tccCCGTCAGCCTACGGCTTCTGCCCGTTTACCGCTGACGCTGATGACGCTTGATGACTGGGCGCGTGCCACCATTACTGGCGCGGACAGCGAAAAATATATGCAGGGTCAGGTGACAGCAGATGTCAGCCAGATGGCagaag < 1:329564/143‑1 (MQ=255) tccCCGTCAGCCTACGGCTTCTGCCCGTTTACCGCCGACGCAGATGACGGTGGAAGACGGGGCGCGTGCCACCATTACTGGCACGGACCGAGAACGGGGTATGCACGGTCAGGTGACAGCAGGCGGCGGCCAGCTgggcggag > 2:506792/1‑137 (MQ=255) ccccGTCAGCCTACGGCTTCTGCCCGTTTACCGCTGACGCTGATGACGCTTGATGACTGGGCGCGTGCCACCATTACTGGCGCGGACAGCGAAAAATATATGCAGGGTCAGGTGACAGCCGATGTCAGCCAGATGGCagagga > 1:185508/1‑143 (MQ=255) gTCAGCCTACGGCTTCTGCCCGTTTACCGCTGACGCTGATGACGCTTGATGACTGGGCGCGTGCCACCATTACTGGCGCGGACAGCGAAAAAGATATGCAGGGTCAGGTGACAGCAGATGTCAGCCAGATGGCAGAAGATCAg > 1:739824/1‑143 (MQ=255) ccTACGGCTTCTGCCCGTTTACCGCTGACGCTGATGACGCTTGATGACTGGGCGCGTGCCACCATTACTGGCGCGGACAGCGAAAAATATATGCAGGGTCAGGTGACAGCAGATGTCAGCCAGATGGCAGAAGATAAGCACCt > 2:1062314/1‑143 (MQ=255) cTACGGCTTCTGCCCGTTTACCGCTGACGCTGCTGACGCTTGATGACTGGGCGCGTGCCACCATTACTGGCGCGGACAGCGAAAAATATATGCAGGGTCAGGTGACAGCAGATGTCAGCCAGATGGCAGCAGATCAGCACCTg > 1:456133/1‑143 (MQ=255) cTACGGCTTCTGCCCGTTTACCGCTGACGCTGATGACGCTTGATGACTGGGCGCGTGCCACCATTACTGGCGCGGACAGCGAAAAATATATGCAGGGTCAGGTGACAGCAGATGTCAGCCAGATGGCAGAAGATCAGCACCTg < 1:550230/143‑1 (MQ=255) gcctccgCCCGGTTACCGCTTCCGCTGACGACGCGTGATGACTGGGCGCGTGCCACCATTACTGGCGCGGACAGCGAAAAATATATGCAGGGTCAGGTGACAGCAGATGTCAGCCAGATGGCAGAAGATCAGCACCTGCTcgc < 2:528640/137‑1 (MQ=255) ttCTGCCCGTTTACCGCTGACGCTGATGACGCTTGATGACTGGGCGCGTGCCACCATTACTGGCGCGGACAGCGAAAAATATATGCAGGGTCAGGCGACTGCAGATGTCAGCCAGATGGCAGCAGATCAGCACCTGCTcgccg > 1:128031/1‑143 (MQ=255) ttCTGCCCGCGTCCCGCTGACGCTGATGACGCTTGATGACTGGGCGCGTGCCACCATTACCGGCGCGGCCACCGAAAAATATCAGACCCTTTAGGTGACAGCAGCTGTCAGCCAGATGGCAGAAGATCAGCACCTGCTcgccg < 2:1246811/143‑1 (MQ=255) tGCCCGTTTACCGCTGACGCTGATGACGCTTGATGACTGGGCGCGTGCCACCATTACTGGCGCGGACAGCGAAAAATATATGCAGGGTCAGGTGACAGCAGATGTCAGCCAGATGGCAGAAGATCAGCACCTGCTCGCCGccc < 2:224802/143‑1 (MQ=255) tGCCCGTTTACCGCTGACGCTGATGACGCTTGATGACTGGGCGCGTGCCACCATTACTGGCGCGGACAGCGAAAAATATATGCAGGGTCAGGTGACAGCAGATGTCAGCCAGATGGCAGAAGATCAGCACCTGCTCGCCGccc < 1:520491/143‑1 (MQ=255) ggCCCGTTTACCGCGGGCGCGGATGACGCGTGATGACTGGGCGCGTGCCACCATTACTGGCGCGGACAGCGAAAAAGATATGCAGGGTCAGGTGACAGCAGATGTCAGCCAGATGGCAGAAGATCAGCACCCGATcgacgtgg < 2:222131/142‑4 (MQ=255) cccGTTTACCGCTGACGCTGATGACGCTTGATGACTGGGCGCGTGCCACCATTACTGGCGCGGACAGCGAAAAATATATGCAGGGTCAGGAGACAGCAGATATCAGCCAGATGGCAGAAGATCAGCACCTGCTCGCCGCCCAt > 2:78074/1‑143 (MQ=255) tcGTTTACCGCTGACGCTGATGACGTCTCATGACTGGGCGCGTGCCACCATTACTGGCGCGGACAGCGGAAAATATATGCAGGGTCAGGTGACAGCAGATGTCAGCCAGATGGCAGAAGATCAGCACCTGCTCGCCGCCCAtt < 2:610265/142‑1 (MQ=255) ccGTTTACCGCTGACGCTGATGACGCTTGATGACTGGGCGCGTGCCACCATTACTGGCGCGGACAGCGAAAAATATATGCAGGGTCAGGTGACAGCAGATGTCAGCCAGATGGCAGAAGATCAGCACCGGCTCGCCGCCCAtt > 2:112326/1‑143 (MQ=255) ccGTTCACCGCTGACGCTGATGACGCTTGATGACTGGGCGCGTGCCACCATTACTGGCGCGGACAGCGAAAAATATATGCAGGGTCAGGTGACAGCAGATGTCAGCCAGATGGCAGAAGATCAGCACCTGCTCGCCGCCCAtt < 1:963084/143‑1 (MQ=255) ttACCGCTGACGCTGATGACGCTTGATGACTGGGCGCGTGCCGCCATTACTGGCGCGGACAGCGAAAAATATATGCAGGGTCAGGTGACAGCAGATGTCAGCCAGATGGCAGAAGATCAGCACCTGCTCGCCGCCCATTGCGa < 2:335841/143‑1 (MQ=255) ttACCGCTGACGCTGATGACGCTTGATGACTGGGCGCGTGCCACCATTACTGGCGCGGACAGCGAAAAATATATGCAGGGTCAGGTGACAGCAGATGTCAGCCAGATGGCAGAAGATCAGCACCTGCTCGCCGCCCATTGCGa < 1:569671/143‑1 (MQ=255) tACCGCTGACGCTGATGACGCTTGATGACTGGGCGCGTGCCACCATTACTGGCGCGGACAGCGAAAAATATATGCAGGGTCAGGTGACAGCAGATGTCAGCCAGATGGCAGAAGATCAGCACCTGCTCGCCGCCCATTGCGAc < 1:734293/143‑1 (MQ=255) cgctgacgctgaTGACGCTTGATGACTGGGCGCGTGCCACCATTACTGGCGCGGACAGCGAAAAATCTATGCAGGGTCAGGTGACAGCAGATGTCAGCCAGATGGCAGAAGATCAGCACCTGCTCGCCGCCCATTGCGACGcc > 1:59926/1‑143 (MQ=255) gacgctgaTGACGCTTGATGACTGGGCGCGTGCCACCATTACTGGCGCGGACAGCGAAAAATATATGCAGGGTCAGGTGACAGCAGATGTCAGCCAGATGGCAGAAGATCAGCACCTGCTCGCCGCCCATTGCGACGCCAAAg < 2:218804/143‑1 (MQ=255) gctgaTGACGCTTGATGACTGGGCGCGTGCCACCATTACTGGCGCGGACAGCGAAAAATATATGCAGGGTCAGGTGACAGCAGATGTCAGCCAGATGGCAGAAGATCAGCACCTGCTCGCCGCCCATTGCGACGCCAAAGGTa > 2:1105823/1‑143 (MQ=255) gctgaTGACGCTTGATGACTGGGAGCGTGCCACCATTACTGGCGCGGACAGCGAAAAAAATATGCAGGGTCAGGTGACAGCAGATGTCAGCCAGAAGGCAGAAGATCAGCACCTGCTCGCCGACCCGTGCGACGCCAAAGGTa > 2:350292/1‑143 (MQ=255) tgaCGCTTGATGACTGGGCGCGTGCCACCATTACTGGCGCGGACAGCGAAAAATATATGCAGGGTCAGGTGACAGCAGATGTCAGCCAGATGGCAGAAGATCAGCACCTGCTCGCCGCCCATTGCGACGCCAAAGGTAAAAtg < 1:189999/143‑1 (MQ=255) aCGCTTGATGACTGGGCGCGTGCCACCATTACTGGCGCGGACAGCGAACAATATATGCAGGGTCAGGTGACAGCAGATGTCAGCCAGATGGCAGAAGATCAGCACCTGCTCGCCGCCCATTGCGACGCCAACGGTAAAAggtg > 1:673182/1‑143 (MQ=255) aCGCTGGATGACTGGGCGCGTGCCACCATTACTGGCGCGGACAGCGAAAAATATATGCAGGGTCAGGTGACAGCAGATGTCAGCCAGATGGCAGAAGATCAGCACCTGCTCGCCGCCCATTGCGACGCCAAAGGTAAAAtgtg < 2:174932/143‑1 (MQ=255) cGCCTGACGACTGCGCGCGTGCCACGAGTAGTGGAGCTCACCGCGTATGACATATGCAGGGTCAGGTGACAGCAGATGTCAGCCAGATGGCAGAAGATCAGCACCTGCTCGCCGCCCATTGCGACGCCAAAGGTAAAATGTgg < 2:1240495/143‑1 (MQ=255) tggccgggCGCGTGCCAGCGTTACGGGCGCGGACAGCGAAAAATATAGGCAGGGTCAGGTGACAGCAGATGTCAGCCAGATGGCAGAAGATCAGCACCTGCTCGCCGCCCATTGCGACGCCAAAGGTAAAATGTGGAGCAAtt < 2:869191/138‑1 (MQ=255) gaCTGGGCGCGTGCCACCATTACTGGCGCGGACAGCGAAAAATATATGCAGGGTCAGGTGACAGCAGATGTCAGCCAGATGGCAGAAGATCAGCACCTGCTCGCCGCCCATTGCGACGCCAAAGGTAAAATGTGGAGCAAttt < 2:251101/143‑1 (MQ=255) gggCGCGTGCCACCAGTACGGGCGCGGAGAGCTAAAAATGTATGCAGGGTCAGGTGACAGCAGATGTCAGCCAGATGGCAGAAGATCAGCACCTGCTCGCCGCCCATTGCGACGCCAAAGGTAATATTTGGAGCAATTTACGt < 2:159285/143‑1 (MQ=255) | AGCTCTTTTACCATTAGTCATTAATACGCCGTTAAGCAACTCAGGACTCTATTATGGCTTTTACACCTTTTCCTCCCCGTCAGCCTACGGCTTCTGCCCGTTTACCGCTGACGCTGATGACGCTTGATGACTGGGCGCTTGCCACCATTACTGGCGCGGACAGCGAAAAATATATGCAGGGTCAGGTGACAGCAGATGTCAGCCAGATGGCAGAAGATCAGCACCTGCTCGCCGCCCATTGCGACGCCAAAGGTAAAATGTGGAGCAATTTACGT > NC_000913/3041260‑3041534 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |