Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,270,143 C→T 11.5% intergenic (+541/+73) yhaC → / ← rnpB uncharacterized protein YhaC/RNase P catalytic RNA component

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,270,1430CT11.5% 106.1 / 3.6 49intergenic (+541/+73)yhaC/rnpBuncharacterized protein YhaC/RNase P catalytic RNA component
Reads supporting (aligned to +/- strand):  ref base C (25/18);  new base T (4/2);  total (29/20)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

ATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAATCAGGTGAAACTGACCGATAAGCCGGGTTCTGTCGTGGACAGTCATTCATCTAGGCCAGCAATCGCTCACT  >  NC_000913/3270005‑3270284
                                                                                                                                          |                                                                                                                                             
aTAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGcgtcg                                                                                                                                           <  1:1160693/143‑1 (MQ=39)
      cGCTCTCTCTCGGGACTAGTGTCGTCGACAGTCGCTCAAATTTCTGCCCCTCCAAAAGCAAAAACCCGCCGGAGCGGGTTATTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACa                                                                                                                                     <  2:1211922/143‑1 (MQ=11)
                   gTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTGTTGGGTACAGCGTCGTGGACAGtcctgcatgcgt                                                                                                                        >  1:971814/1‑139 (MQ=11)
                     ataGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGTCCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCt                                                                                                                      <  1:666374/143‑1 (MQ=38)
                           tCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCGTTCTGTTGGGTATAGCGGCGTGGACAGTCAATCATCTCTCTGccccg                                                                                                                >  1:462885/1‑142 (MQ=14)
                                   aGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTCCGTAAACCAGGTGAACCTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGGCAGTCATCTTTCTGCCCCTCCAAAAGc                                                                                                        >  1:125044/1‑143 (MQ=255)
                                     tcattcatCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCaa                                                                                                      >  1:1277576/1‑143 (MQ=38)
                                       attctccTTTCTGCCCCTCCGAAAGCAAAAACCCGCCGAAGCGGTTTTTTACGTAAATCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCaaaa                                                                                                    <  2:413174/137‑1 (MQ=11)
                                              cgtcctccccACCCAAACCAAAACCCCGCCGAACCGGGTGTGTACGGACACCCGGTGAACCTGACCGATAAGCCGCTGTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACccgcc                                                                                             <  1:1066120/137‑1 (MQ=11)
                                               ttCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGGTTTCTTTTGGGTATAGCGACGCGGACAGGCCATCATCCTTCCGCCGCCCCAAACGCACAAACccgccg                                                                                            >  2:357502/1‑143 (MQ=14)
                                                     cccTCCGAGTGCTAAGACCCGCCGGAGCGGGTTTTTACGTAAATCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCgg                                                                                      <  2:215421/143‑1 (MQ=11)
                                                     cccTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGTGGATAGCGATGTAGCCAGTCAATCAACCTTTTGCCCCCCCCAAAGCACAAACCCGCCGGCGCgg                                                                                      >  2:1128731/1‑143 (MQ=14)
                                                      acTCCACAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAAGCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCggg                                                                                     <  2:1040116/142‑1 (MQ=21)
                                                             aaGCAAAAACCCGCCGAAACGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTAc                                                                              <  2:1186252/143‑1 (MQ=37)
                                                                      cccGCCGAAGCGGGTTTTTACGTAAACCAGGTAAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAATCAg                                                                     <  2:354746/143‑1 (MQ=37)
                                                                           cgAAGAGGTTTGTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAATCAGGTGaa                                                                <  2:477541/143‑1 (MQ=255)
                                                                                   gTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGGTTTTACGTAAAGCAAGTGAAACTGAcgg                                                        >  1:888198/1‑141 (MQ=255)
                                                                                     ttttACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAATCAGGTGAAACTGACCGAt                                                      >  1:117508/1‑143 (MQ=38)
                                                                                       ttACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCtt                                                                                                                         <  1:392970/74‑1 (MQ=255)
                                                                                           gTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTCCTGCCCCGCCAAAAGCAAAAACCAGCCGACGCGGGGTTTTAAGTAAACCAGGTGCAACTGACCGATAAGCCg                                                >  2:1033278/1‑143 (MQ=11)
                                                                                             aaaCCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCAGTTTTCTGCCCCTCCAAAAGCACACACCCGCCGAAGCGGGGTGTTACGTAAACCAGGTGACACTCACCGATAAGcgcgg                                              >  2:990794/1‑139 (MQ=11)
                                                                                                   ggTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAATTAGGCGAAACTGACCGATAGGCCGGGTTCtct                                        >  2:1234546/1‑141 (MQ=255)
                                                                                                    gTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGcccc                                                                                                                 <  1:926863/69‑1 (MQ=37)
                                                                                                    gTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCACACGCAAACACCCGCCGCAGCGGGTTTTTTCGTACATCCGGTGAAACTGACCAATAAACCGAACTCTGTc                                       >  1:454695/1‑143 (MQ=11)
                                                                                                    gTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAATCAGGTGAAACTGACCGATAAGCCGGGTTCTGTc                                       >  1:454451/1‑143 (MQ=255)
                                                                                                     tGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAATCAGGTGAAACTGACCGATAAGCCGGGTTCTGTCg                                      >  2:517701/1‑143 (MQ=38)
                                                                                                     tGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGCAAATCAGGCGAAACTGACCGATACGCCGGGTTCTGTCg                                      >  2:939343/1‑143 (MQ=255)
                                                                                                         aCTGACCGATAAGCCGCTTTCTTGTGGGTATAGCGGCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAATCAGGTGAAACTGACCGATAAGCCGGGTTCTGTCGTGGa                                  <  1:1159222/143‑1 (MQ=255)
                                                                                                           tGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAATCAGGTGAAACTGACCGATAAGCCGGGTTCTGTCGTGGACa                                <  1:517701/143‑1 (MQ=255)
                                                                                                            gACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAACACCCGCCGAAGCGGGTTCTTACGTAAACCGGGTGAACCTGACCGATAAGCCGGGTTCTGTCGAGGatcg                               >  2:281114/1‑140 (MQ=11)
                                                                                                             aCCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGGTTTTACGTAAATCAGGTGAAACTGACCGATAAGCCGGGTTCTGTCGTGGACAgg                              >  1:68343/1‑142 (MQ=255)
                                                                                                              ccGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGNACAGTCAGTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAATCAGGTGAAACTGACCGATAAGCCGGGTTCTGTCGTGGACAGtc                             <  1:720424/143‑1 (MQ=255)
                                                                                                                 aTAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGGGTTCTTTCGTGGACAgcgatg                          >  2:1071373/1‑138 (MQ=11)
                                                                                                                 aTAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAATCAg                                                                     <  1:472034/100‑1 (MQ=38)
                                                                                                                 aTAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAATCAGGTGAAACTGACCGCTAAGCCGGGTTCTGTCGTGGACAGtcctt                          >  1:1049872/1‑143 (MQ=255)
                                                                                                                     gCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAATCAGGTGAAACTGACCGATAAGCCGGGTTCTGTCGTGGACAGTCATTCATc                      >  2:525125/1‑143 (MQ=255)
                                                                                                                      ccGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCTCAAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAATCAGGTGAAACTGACCGATAAGCCGGGTTCTGTCGTGGACAGTCATTCATCt                     >  1:116722/1‑143 (MQ=255)
                                                                                                                      ccGCTTTCTTTTGGGTATAGCGTCGTGGACAGGCATGCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAATCAGGTGAAACTGACCGATAAGCCGGGTTCTGTCGTGGACAGTCATTCATCt                     >  2:778631/1‑143 (MQ=255)
                                                                                                                        gCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAATCAGGTGAAACTGACCGATAAGCCGGGTTCTGTCGTGGACAGTCATTCATCTAg                   <  1:826584/143‑1 (MQ=255)
                                                                                                                        gCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAATCAGGTGAAACTGACCGATAAGCCGGGTTCTGTCGTGGACAGTCATTCATCTAg                   >  1:549480/1‑143 (MQ=255)
                                                                                                                          tttttgttGGCTATAGTGGCGTGGACAGTCATGCATCTTTCTGCCCCTCAAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAATCAGGTGAAACTGACCGATAAGCCGGGTTCTGTCGTGGACAGTCATTCATCTAGGc                 <  2:658046/137‑1 (MQ=17)
                                                                                                                             ctttTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCGCCAAACGCGCAAACCCGCCGAACCGGGTTTTCATGTAAATCAGGTGAACCTCACCGATATGCCGGATTCTGTCGTGGACCGCCGCTCACCGAGGCCAg              >  2:1031905/1‑143 (MQ=14)
                                                                                                                              ttttGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAATCAGGTGAAACTGACCGATAAGCCGGGTTCTGTCGGGGATAGCCATTTAGCCAGGCCAGc             >  2:1251972/1‑143 (MQ=14)
                                                                                                                              ttttGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAATCAGGTGAAACTGACCGATAAGCCGGGTTCTGTCGTGGACTGTCATTCATCTACGCCAGc             >  1:719949/1‑143 (MQ=255)
                                                                                                                              ttttGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAATCAGGTGAAACTGACCGATAAGCCGGGTTCTGTCGTGGACAGTCATTCATCTAGGCCAGc             >  2:781581/1‑143 (MQ=255)
                                                                                                                              ttttGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAATCAGGTGAAACTGACCGATAAGCCGGGTTCTGTCGTGGACAGCCATTCATCTAGGCCAGc             >  2:373975/1‑143 (MQ=255)
                                                                                                                              ttttGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCAGCTCGAAAAGCAAAGACACGCCGAAGCGGGTTTGTACGTAAATCAGGTGAAACTGACCGCTAAGCGGGGTTATGTCAGGGACAGTCATTCATGTAGGCCAga             >  2:401744/1‑142 (MQ=17)
                                                                                                                                gtGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAATCAGGTGAAACTGACCGATAAGCCGGGTTCTGTCGTGGACAGTCATTCATCTAGGCCAGCaa           <  1:484406/142‑1 (MQ=255)
                                                                                                                                   ggTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGc                                                                                        <  2:579756/63‑1 (MQ=35)
                                                                                                                                         gCGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAATCAGGTGAAACTGACCGATAAGCCGGGTTCTGTCGTGGACAGTCATTCATCTAGGCCAGCAATCGCTCACt  <  1:10115/143‑1 (MQ=255)
                                                                                                                                          |                                                                                                                                             
ATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAATCAGGTGAAACTGACCGATAAGCCGGGTTCTGTCGTGGACAGTCATTCATCTAGGCCAGCAATCGCTCACT  >  NC_000913/3270005‑3270284

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑