| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_000913 | 3,740,791 | G→C | 7.3% | G363R (GGC→CGC) | avtA → | valine‑‑pyruvate aminotransferase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 3,740,791 | 0 | G | C | 7.3% | 162.2 / 5.7 | 58 | G363R (GGC→CGC) | avtA | valine‑‑pyruvate aminotransferase |
| Reads supporting (aligned to +/- strand): ref base G (31/22); new base C (2/2); total (34/24) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.91e-01 | |||||||||||
ATCGCCATCATTCGCCGCTATTTACCGGAAAATCGCTGCCTGATTCATAAACCGGAAGGAGCCATTTTCCTCTGGCTATGGTTTAAGGATTTGCCCATTACGACCAAGCAGCTCTATCAGCGCCTGAAAGCACGCGGCGTGCTGATGGTGCCGGGGCACAACTTCTTCCCAGGGCTGGATAAACCGTGGCCGCATACGCATCAATGTATGCGCATGAACTACGTACCAGAGCCGGAGAAAATTGAGGCGGGGGTGAAGATTCTGGCGGAAGAGATAG > NC_000913/3740656‑3740932 | aTCGCCATCATTCGCCGCTATTTACCGGAAAATCGCTGCCTGATTCATAAACCGGAAGGAGCCATTTTCCTCTGGCTATGGTTTAAGGATTTGCCCATTACGACCAAGCAGCTCTATCAGCGCCTGAAAGCACGCGGCGTGCt > 1:967172/1‑143 (MQ=255) gCCATCATTCGCCGCTATTTACCGGAAAATCGCTGCCTGATTCATAAACCGGAAGGAGCCATTTTCCTCTGGCTATGGTCTAAGGATTTGCCCATTACGACCAAGCAGCTCTATCAGCGCCTGAAAGCACGCCGCGTGCtttc < 1:1076434/143‑4 (MQ=255) catcatTCGCCGCTATTTACCGGAAAATCGCTGCCTGATTCATAAACCGGAAGGAGCCATTTTCCTCTGGCTATGGTTTAAGGATTTGCCCATTACGACCAAGCAGCTCTATCAGCGCCTGAAAGCACGCCGCGTGCtttcag < 1:915424/143‑6 (MQ=255) tCGCCGCTATTTACCGGAAAATCGCTGCCTGATTCATAAACCGGAAGGAGCCATTTTCCTCTGGCTATGGTTTAAGGATTTGCCCATTACGACCAAGCAGCTCTATCAGCGCCTGAAAGCACGCGGCGTGCTGATGGTGCCgg > 1:393750/1‑143 (MQ=255) gccgccATTTACCGGAAAGTCGCGTCCTGATTCATAAAGCGGACGGAGCCATTTTCCTCTGGCTATGGTTTAAGGATTTGCCCATTACGACCAAGCAGCTCTATCAGCGCCTGAAAGCACGCGGCGTGCTGATGGTGCCgggg < 1:349353/143‑1 (MQ=255) ctcTATTTACCCGAAAATCCCTGCCTGATTCATAAACCGGAAGGAGCCATTTTCCTCTGGCTATGGTTTAAGGATTTGCCCATTACGACCAAGCAGCTCTATCAGCGCCTGAAAGCACGCGGCGTGCTGATGGTGCCGGGGca < 2:982575/141‑1 (MQ=255) tttACCGGAAAATCGCTGCCTGATTCATAAACCGGAAGGAGCCATTTTCCTCTGGCTATGGTTTAAGGATTTGCCCATTACGACCAAGCAGCTCTATCAGCGCCTGAAAGCACGCGGCGTGCTGATGGTGCCGGGGCACAAct > 1:767358/1‑143 (MQ=255) ggAAAATCGCTGCCTGATTCATAAACCGGAAGGAGCCATTTTCCTCTGGCTGTGGTTTAAGGATTTGCCCATTACGACCAAGCAGCTCTATCAGCGACTGAAGGCACGCGGCGTGCTGATGGTGCAGTGGCACAActtcttga > 2:745103/1‑141 (MQ=255) gAAAATCGCTGCCTGATTCATAAACCGGAAGGAGCCATTTTCCTCTGGCTATGGTTTAAGGATTTGCCCATTACGACCAAGCAGCTCTATCAGCGCCTGAAAGCACGCGGCGTGCTGATGGTGACGGGGGTAAACTTCGTccc > 1:791377/1‑143 (MQ=255) aagaTCGCTGCCTGATTCATAAACCGGAAGGAGCCATTTTCCTCTGGCTATGGTTTAAGGATTTGCCCATTACGACCAAGCAGCTCTATCAGCGCCTGAAAGCACGCGGCGTGCTGATGGTGCCGGGGCACAACTTCTTCCCa < 2:63129/140‑1 (MQ=255) aaaaTCGCTGCCTGATTCATAAACCGGAAGGAGCCATTTTCCTCTGGCTATGGTTTAAGGATTTGCCCATTACGACCAAGCAGCTCTATCAGCGCCTGAAAGCACGCGGCGTGCTGATGGTGCCGGGGCACAACTTCTTCCCa < 2:233270/143‑1 (MQ=255) aaaaTCGCTGCCTGATTCATAAACCGGAAGGAGCCATTTTCCTCTGGCTATGGTTTAAGGATTTGCCCATTACGACCAAGCAGCTCTATCAGCGCCTGAAAGCACGCGGCGTGCTGATGCTGCCGGGGCACAACTTCTTCCCa > 2:614857/1‑143 (MQ=255) aaaaTCGCTGCCTGATTCATAAACCGGAAGGAGCCATTTTCACATCGATAAGGTTTGAGGATTTGCCCATTACGACCACGCAGCTCTATCAGCGCCTGAAAGCACGCGACGTGCTGGTGGTGCCGGGGCCCCACTTCTTcccg > 2:273433/1‑142 (MQ=255) caaTCGCGGCCTGCTGCATGAACCGGAAGGAGCCATTTTCCTCTGGCTATGGCTTAAGGATTTGCCCATTACGACCAAGCAGCTCTATCAGCGCCTGAAAGCACGCGGCGTGCTGATGGTGCCGGGGCACAACTTCTTCCCAg < 2:365797/142‑1 (MQ=255) gCTGCCTGATTCATAAACCGGAAGGAGCCATTTTCCTCTGGCTATGGTTTAAGGATTTGACCATTACGACCAAGAAGCTCTATCAGCGCCTGAAAGCACGCGGCGTGCTGATGGTGACGGGGCACAACTTCTTCCCAGGGCTg > 2:369441/1‑143 (MQ=255) cTGCCTGATTCATAAACCGGAAGGAGCCATTTTCCTCTGGCTATGGTTTAAGGATTTGCCCATTACGACCAAGCAGCTCTATCAGCGCCTGAAAGCACGCGGCGTGCTGATGGTGCCGGGGCACAACTTCTTCCCAGGGCTgg < 1:965531/143‑1 (MQ=255) tGCCTGATTCATAAACCGGAAGGAGCCATTTTCCTCTGGCTATGGTTTAAGGATTTGCCCATTACGACCAAGCAGCTCTATCAGCGCCTGAAAGCACGCGGCGTGCTGATGGTGCCGGGGCACAACTTCTTCCCAGGGCTGGa < 1:975431/143‑1 (MQ=255) ccTGATTCATAAACCGGAAGGAGCCATTTTCCTCTGGCTATGGTTTAAGGATTTGCCCATTACGACCAAGCAGCTCTATCAGCGCCTGAAAGCACGCGGCGTGCTGATGGTGCCGGGGCACAACTTCTTCCCAGGGCTGGATa > 1:949039/1‑143 (MQ=255) tGATTCATAAACCGGAAGGAGCCATTTTCCTCTGGCTATGGTTTAAGGATTTGCCCATTACGACCAAGCAGCTCTATCAGCGCCTGAAAGCACGCGGCGTGCTGATGGTGCCGGGGCACAACTTCTTCCCAGGGCTGGATaaa < 1:154472/143‑1 (MQ=255) aTTCATAAACCGGAAGGAGCCATTTTCCTCTGGCTATGGTTTAAGGATTTGCCCATTACGACCAAGCAGCTCTATCAGCGCCTGAAAGCACGCGGCGTGCTGATGGTGCCGGGGCACAACTTCTTCCCAGGGCTGGATAAAcc > 1:352625/1‑143 (MQ=255) aTTCATAAACCGGAAGGAGCCATTTTCCTCTGGCTATGGTTTAAGGATTTGCCCATTACGACCAAGCAGCTCTATCAGCGCCTGAAAGCACGCGGCGTGCTGATGGTGCCGGGGCACAACTTCTTCCCAGGGCTGGATAAAcc > 2:90735/1‑143 (MQ=255) aTAAACCGGAAGGAGCCATTTTCCTCTGGCTATGGTTTAAGGATTTGCCCATTACGACCAAGCAGCTCTATCAGCGCCTGAAAGCACGCGGCGTGCTGATGGTGCCGGGGCACAACTTCTTCCCAGGGCTGGATAAACCGTgg > 1:1251178/1‑143 (MQ=255) tAAACCGGAAGGAGCCATTTTCCTCTGGCTATGGTTTAAGGATTTGCCCATTACGACCAAGCAGCTCTATCAGCGCCTGAAAGCACGCGGCGTGCTGATGGTGCCGGGGCACAACTTCTTCCCAGGGCTGGATAAACCGTGGc > 2:197229/1‑143 (MQ=255) aaaCCGGAAGGAGCCATTTTCCTCTGGCTATGGTTTAAGGATTTGCCCATTACGACCAAGCAGCTCTATCAGCGCCTGAAAGCACGCGGCGTGCTGATGGTGCCGGGGCACAACTTCTTCCCAGGGCTGGATAAACCGTGGcc > 2:1137865/1‑143 (MQ=255) ccGGAAGGAGCCATTTTCCTCTGGCTATGGTTTAAGGATTTGCCCATTACGACCAAGCAGCTCTATCAGCGCCTGAAAGCACGCGGCGTGCTGATGGTGCCGGGGCACAACTTCTTCCCAGGGCTGGATAAACAGTGGCCGCa > 1:151068/1‑143 (MQ=255) cGGAAGGAGCCATTTTGCAGTGGCTATGGTTTAAGGATTGGCTGATTACGACGAAGCAGCTCTATCAGCGCCTGAAAGCACGCGGCGTGCTGATGGTGCCGGGGCACAACTTCTTCCCAGGGCTGGATAAACCGTGGCCGCAt < 1:1188494/143‑1 (MQ=255) cGGAAGGAGCCATTTTCCTCTGGCTATGGTTTAAGGATTTGCCCATTACGACCAAGCAGCTCTATCAGCGCCTGAAAGCACGCGGCGTGCTGATGGTGCCGGGGCACAACTTCTTCCCAGGGCTGGATAAACCGTGGCCGCAt > 1:1165339/1‑143 (MQ=255) aGCCATTTTCCTCTGGCTATGGGTTAAGGATTTGCCCATTACGACCAAGCAGCTCTATCGGCGCCTGAAAGCACGCGGCGTGCTGATGGTGCCGGGGCACAACTTCTTCCCAGGGCTGGATAAACCGTGGCCGCATACGCATc < 2:861247/143‑1 (MQ=255) gCCATTTTCCTCTGGCTATGGTTTAAGGATTTGCCCATTACGACCAAGCAGCTCTATCAGCGCCTGAAAGCACGCGGCGTGCTGATGGTGCCGGGGCACAACTTCTTCCCAGGGCTGGATAAACCGTGGCCGCATACGCATCa < 2:683698/143‑1 (MQ=255) ccATTTTCCTCTGGCTATGGTTTAAGGATTTGCCCATTACGACCAAGCAGCTCTATCAGCGCCTGAAAGCACGCGGCGTGCTGATGGTGCCGGGGCACAACTTCTTCCCAGGGCTGGATAAACCGTGGCCGCATACGCATCac > 1:878668/1‑142 (MQ=255) ttCCTCTGGCGATGGTTTAAGGGTTTGGCGATTGTGAGGAAGCAGTTCTGTCGGGGCGTGAGCGCGCGCGTCGTCCTGTTGGTGCCCTCACACAACTTCTTCCCAGGGCTGGATAAACCGTGGCCGCATACGCATCAATGTAt < 2:907989/143‑1 (MQ=255) ctGGCTATGGTTTAAGGATTTGCCCATTACGACCAAGCAGCTCTATCAGCGCCTGAAAGCACGCGGCGTGCTGATGGTGCCGGGGCACAACTTCTTCCCAGGGCTGGATAAACCGTGGCCGCATACGCATCAATGTATGCGCa > 1:885431/1‑143 (MQ=255) gTTTAAGGATTTGCCCATTACGACCAAGCAGCTCTATCAGCGCCTGAAAGCACGCGGCGTGCTGATGGTGCCGGGGCACAACTTCTTCCCAGGGCTGGATAAACCGTGGCCGCCTACGCATCACTGTATGCGCATGAACTACg > 1:335919/1‑143 (MQ=255) ttAAGGATTTGCCCATTACGACAAGGCCGCCCTTTCAGCGCTTGACAGCAAGAAAACGGCATTTGGTGGCGGCAGTCGTCATCGTCCCGGGGCTGGAGAAAGCGGGGCCGCATACTCATCAATGTATGCGGATGAACTACGTa > 2:568778/1‑143 (MQ=255) tAAGGATTTGCCCATTACGCCCAAGCAGCTCTATCAGCGCCTGAAAGCACGCGGCGTGCTGATGGTGCCGGGGCACAACTTCTTCCCAGGGCTGGATAAACCGTGGCCGCATACGCATCAATGTATGCGCATGAACTACGTAc < 2:1229090/143‑1 (MQ=255) ccATTACGACCAAGCAGCTCTATCAGCGCCTGAAAGCACGCGGCGTGCTGATGGTGCCGGGGCCCAACTTCTTCCCAGGGCTGGATAAACCGTGCCCGCATCCCCATCAATGCATGCGCATGAACTACGCACCAGAGCCgagg > 2:1230048/1‑140 (MQ=255) ccATTACGACCAAGCAGCTCTATCAGCGCCTGAAAGCACGCGGCGTGCTGATGGTGCCGGGGCACAACTTCTTCCCAGGGCTGGATAAACCGTGGCCGCATACGCATCAATGTATGCGCATGAGCTAAGTACCAGAGCCGgag > 2:250526/1‑143 (MQ=255) ttACGACCAAGCAGCTCTATCAGCGCCTGAAAGCACGCGGCGTGCTGATGGTGCCGGGGCACAACTTCTTCCCAGGGCTGGATAAACCGTGGCCGCATACGCATCAATGTATGCGCATGCACTACGTAGCCGACCCGgagaga > 2:963000/1‑141 (MQ=255) ggtcGACCATGCAGCTCTAGCGGCGCCTGAAAGCACGCGGCGTGCTGATGGTGCCGGGGCACAACGTCTTCCCAGGGCTGGATAAACCGTGGCCGCATACGCATCAATGTATGCGCATGAACTACGTACCAGAGCCGGAGaaa < 2:508822/140‑1 (MQ=255) cAAGCAGCTCTATCAGCGCCTGAAAGCACGCGGCGTGCTGATGGTGCCGGGGCACAACTTCTTCCCAGGGCTGGATAAACCGTGGCCGCATACGCATCAATGTATGCGCATGAACTACGTACCAGAGCCGGAGAAAATTGAgg > 1:899833/1‑143 (MQ=255) cAAGCAGCTCTATCAGCGCCTGAAAGCACGCGGCGTGCTGATGGTGCCGGGGCACAACTTCTTCCCAGGGCTGGATAAACCGTGGCCGCATACGCATCAATGTATGCGCATGAACTACGCACCAGAGCCGGAGAAAAttacgg > 2:527603/1‑139 (MQ=255) cAAGCAGCTCTATCAGCGCCTGAAAGCACGCGGCGTGCTGATGGTGCCGGGGCACAACTTCTTCCCAGGGCTGGATAAACCGTGGCCGCATACGCATAAATGTATGCGCATGAACTACGTACCAGAGCCGGAGAAAATTGAgg > 1:1064101/1‑143 (MQ=255) agcagcTCTAGCAGCGCCTGAAAGCACGCGGCGTGCTGATGGTGCCGGGGCACAACTTCTTCCCAGGGCTGGATAAACCGTGGCCGCATACGCATCAATGTATGCGCATGAACTACGTACCAGAGCCGGAGAAAATTGAGGCg < 1:838148/143‑1 (MQ=255) gcagcTCTATCAGCGCCTGAAAGCACGCGGCGTGCTGATGGTGCCGGGGCACAACTTCTTCCCAGGGCTGGATAAACCGTGGCCGCATACGCATCAATGTATGCGCATGAACTACGTACCAGAGCCGGAGAAAATTGAGGCgg > 1:829780/1‑143 (MQ=255) cggcTCTCTCCGCCCCTGCAAGCACGCGGCGTGTTGATGGCGCCGGGGCACAACTTCTCCCCAGGGCTGGATAAACCGTGGCCGCATACGCATCAACGTACGCGCATGAACTACGTACCAGAGCCGGAGAAAATTGAGGCggg < 2:1044910/141‑1 (MQ=255) ctctATCAGCGCCTGAAAGCACGCGGCGTGCTGATGGTGCCGGGGCACAACTTCTTCCCAGGGCTGGATAAACCGTGGCCGCATACGCATCAATGTATGCGCATGAACTACGTACCAGAGCCGGAGCAAATTGAGGCGGGGGt > 2:1212129/1‑143 (MQ=255) tggcAGCGGATGAGAGCCCGCGGTGTGCGGATGGTGCCGGGGCACAACTTCTGCGCAGGGCTGGCTAAACCGTGGCCGCATACGCAGGAATGTATGCGCATGAACTACGTACCAGAGCCGGAGAAAATTGAGGCGGGGGTGaa < 2:199188/140‑1 (MQ=255) tATCAGCGCCTGAAAGCACGCGGCGTGCTGATGGTGCCGGGGCACAACTTCTTCCCAGGGCTGGATAAACCGTGGCCGCATACGCATCAATGTATGCGCATGAACTACGTACCAGAGCCGGAGAAAATTGAGGCGGGGGTGaa > 2:476894/1‑143 (MQ=255) aGCGCCTGAAAGCACGCGGCGTGCTGATGGTGCCGGGGCACAACTTCTTCCCAGGGCTGGATAAACCGTGGCCGCATACGCATCAATGTATGCGCATGAACTACGTACCAGAGCCGGAGAAAATTGAGGCGGGGGTGAAGAtt > 2:541618/1‑143 (MQ=255) aGCGCCCGAAAGCACGCGGCGTGCTGATGGTGCCGGGGCACAACTTCTTCCCAGGGCTGGATAAACCGTGGCCGCATACGCATCAATGTATGCGCATGAACTACGTACCAGAGCCGGAGAAAATTGAGGCGGGGGTGAAGAtt < 2:116698/143‑1 (MQ=255) gcgcCTGAAAGCACGCGGCGTGCTGATGGTGCCGGGGCACAACTTCTTCCCAGGGCTGGATAAACCGTGGCCGCATACGCATCAATGTATGCGCATGAACTACGTACCAGAGCCGGAGAAAATTGAGGCGGGGGTGAAGATTc < 1:1013826/143‑1 (MQ=255) gcCTGAAAGCACGCGGCGTGCTGATGGTACCGGGGCACAACGTCATCCCAGGCCGGGATAAACCGTGGCAGCAGACGCATCAATGTATGCGCATGAACTACGTACCAGAGCCGGAGAAAATTGAGGCGGGGGTGAAGATTCTg < 2:441178/143‑1 (MQ=255) cTGAAAGCACGCGGCGTGCTGATGGTGCCGGGGCACAACTTCTTCCCAGGGCTGGATAAACCGTGGCCGCATACGCATCAATGTATGCGCATGAACTACGTACCATAGCCGGAGAAAATTGAGGCGGGGGTGAAGATTCTGGc < 1:1055256/143‑1 (MQ=255) atcaGCACGCCGCGTGCTGATGGTGCCGGGGCACAACTTCTTCCCAGGGCTGGATAAACCGTGGCCGCATACGCATCAATGTATGCGCATGAACTACGTACCAGAGCCGGAGAAAATTGAGGCGGGGGTGAAGATTCTGGCgg > 2:1148404/4‑143 (MQ=255) tcaGCACGCCGCGTGCTGATGGTGCCGGGGCACAACTTCTTCCCAGGGCTGGATAAACCGTGGCCGCATCCGCATCAATGTAAGCGCACGAACTACGTACCAGAGCCGGAGAAAACCGAGGCGGGGGTGAAGATGCGggagga > 2:620539/3‑143 (MQ=255) aaGCACGCGGCGTGCTGATGGTGCCGGGGCACAACTTCTTCCAAGGGCTGGATAAACCGTGGCAGCATCAGCGTCACTGTATGCGCATGAACTACGTACCAGAGCCGGCGAAAGTTGAGGCGGGGGTGAAGATTCTGGCGGaa > 1:24075/1‑143 (MQ=255) gctcggggcgTGCTGGGGGTGCCGGGGCACAGCTGCTGGGCAGGGGTGGATGGACCGTGGCCGCAGGCGCGTCAATGTAGGCGCGTGAAGTACGTACCAGAGCCGGAGAAAATTGAGGCGGGGGTGAAGATTCTGGCGGAaga < 2:1002115/137‑1 (MQ=255) aCGCGGCGTGCTGATGGTGCCGGGGCACAACTTCTTCCCAGGGCTGGATAAACCGTGGCCGCATACGCATCAATGTATGCGCATGAACTACGTACCAGAGCCGGAGAAACTTGAGGCGGGGGTGAAGATTCTGGCGGAAgaga > 2:760652/1‑143 (MQ=255) aCGCGGCGTGCTGATGGTGCCGGGGCACAACTTCTTCCCAGGGCTGGATAAACCGTGGCCGCATACGCATCAATGTATGCGCATGAACTACGTACCAGAGCCGGAGAAAATTGAGGCGGGGGTGAAGATTCTGGCGGAAgaga < 1:1170467/143‑1 (MQ=255) gcggcgTGCTGATGGTGCAGGGGCACAACTTATTCCCAAGGATGGATAAACCGGGGCCGCATACGAATCAATGTAGGCGCATGAACGACGTACCAGAGCCGGAGAAAATTGAGGGGGGGGTGAAGATTCTGGCGGAAGAGATa < 1:15894/143‑1 (MQ=255) cggcgTGCTGATGGTGCCGGGGCACAACTTCTTCCCAGGGCTGGATAAACCGTGGCCGCATACGCATCAATGTATGCGCATGAACTACGTACCAGAGCCGGAGAAAATTGAGGCGGGGGTGAAGATTCTGGCGGAAGAGATAg < 2:791377/143‑1 (MQ=255) cggcgTGCTGATGGTGCCGGGGCACAACTTCTTCCCAGGGCTGGATAAACCGTGGCCGCATACGCATCAATGTATGCGCATGAACTACGTACCAGAGCCGGAGAAAATTGAGGCGGGGGTGAAGATTCTGGCGGAAGAGATAg < 1:197229/143‑1 (MQ=255) | ATCGCCATCATTCGCCGCTATTTACCGGAAAATCGCTGCCTGATTCATAAACCGGAAGGAGCCATTTTCCTCTGGCTATGGTTTAAGGATTTGCCCATTACGACCAAGCAGCTCTATCAGCGCCTGAAAGCACGCGGCGTGCTGATGGTGCCGGGGCACAACTTCTTCCCAGGGCTGGATAAACCGTGGCCGCATACGCATCAATGTATGCGCATGAACTACGTACCAGAGCCGGAGAAAATTGAGGCGGGGGTGAAGATTCTGGCGGAAGAGATAG > NC_000913/3740656‑3740932 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |