BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	3,797,336	C→A	5.7%	S130* (TCA→TAA)	waaL →	O‑antigen ligase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,797,336	0	C	A	5.7%	381.7 / 5.0	134	S130* (TCA→TAA)	waaL	O‑antigen ligase
Reads supporting (aligned to +/- strand): ref base C (63/62); new base A (2/7); total (65/69)
Fisher's exact test for biased strand distribution p-value = 1.67e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 137 total aligned reads displayed.

TTGCTTGATTTAATTTGGTATTCTGCGTTTAAAGTAGATAATTCGCCATTTCGTGCTACTTACCATAGTTATTTAAATACTGCCAAAATATTTATATTTGGTTCTTTTATTGTTTTCTTGACACTAACTAGCCAGCTAAAATCAAAAAAAGAGAGTGTATTATACACTTTGTATTCTCTGTCATTTCTAATTGCTGGATATGCAATGTATATTAATAGCATTCATGAAAATGACCGCATTTCTTTTGGTGTAGGAACGGCAACAGGAGCAGCATATTCAACAATG  >  NC_000913/3797194‑3797478

ttgcttgATTTAATTTGGTATTCTGCGTTTAAAGTAGATAATTCGCCATTTCGTGCTACTTACCATAGTTATTTAAATACTGCCAAAATATTTATATTTGGTTCTTTTATTGTTTTCTTGACACTAACTAACCAGCTAAAATc                                                                                                                                                >  2:879878/1‑143 (MQ=255)

  gcttgATTTAATTTGGTATTCTGCGTTTAAAGTAGATAATTCGCCATTTCGTGCTACTTACCATAGTTATTTAAATACTGCCAAAATATTTATATTTGGTTCTTTTATTGTTTTCTTGACACTAACTAGCCAGCTAAAATCaa                                                                                                                                              <  1:329663/143‑1 (MQ=255)

   cttgATTTAATTTGGTATTCTGCGTTTAAAGTAGATAATTCGCCATTTCGTGTTTGGTACCACAGTTGTTTGCACACCGCCAAAATATTTATATTTGGTTCTTTTATTGTTTTCTTGACACTAACTAGCCAGCTAAAATCaaa                                                                                                                                             >  1:594712/1‑143 (MQ=255)

   cttgATTTAATTTGGTATTCTGCGTTTAAAGTAGATAATTCGCCATTTCGTGCTACTTACCATAGTTATTTAAATACTGCCAAAATATTTATATTTGGTTCTTTTATTGTTTTCTTGACACTAACTAGCCAGCTAAAATCaaa                                                                                                                                             >  2:1144440/1‑143 (MQ=255)

      gATTTAATTTGGTATTCTGCGTTTAAAGTAGATAATTCGCCATTTCGTGCTACTTACCATAGTTATTTAAATACTGCCAAAATATTTATATTTGGTTCTTTTATTGTTTTCTTGACACTAACTAGCCAGCTAAAATCaaaaaa                                                                                                                                          <  2:408006/143‑1 (MQ=255)

       aTTTAATTTGGTATTCTGCGTTTAAAGTAGATAATTCGCCATTTCGTGCTACTTACCATAGTTATTTAAATACTGCCAAAATATTTATATTTGGTTCTTTTATTGTTTTCTTGACACTAACTAGCCAGCTAAAATCaaaaaaa                                                                                                                                         >  2:957934/1‑143 (MQ=255)

        tttAATTTGGTATTCTGCGTTTAAAGTAGATAATTCGCCATTTCGTGCTACTTACCATAGTTATTTAAATACTGCCAAAATATTTATATTTGGTTCTTTTATTGTTTTCTTGACACTAACTAGCCAGCTAAAATCaaaaaaac                                                                                                                                        >  2:870426/1‑142 (MQ=255)

           aagttGGTATTCAGCGTTTAAAGTAGATAATTCGACATTTAGTGCTACATACCATAGTTATTTAAATACTGCCAAAATATTTATATTTGGTTCTTTTATTGTTTTCTTGACACTAACTAGCCAGCTAAAATCAAAAAAagaga                                                                                                                                     <  2:219098/140‑1 (MQ=255)

           aaTTTGGTATTCTGCGTTTAAAGTAGATAATTCGCCATTTCGTGCTACTTACCATAGTTATTTAAATACTGCCAAAATATTTATATTTGGTTCTTTTATTGTTTTCTTGACACTAACTAGCCAGCTAAAATCAAAAAAagaga                                                                                                                                     <  1:310800/143‑1 (MQ=255)

           aaTTTGGTATTCTGCGTTTAAAGTAGATAATTCGCCATTTCGTGCTACTTACCATAGTTATTTAAATACTGCCAAAATATTTATATTTGGTTCTTTTATTGTTTTCTTGACACTAACTAGCCAGCTAAAATCAAAAAAagaga                                                                                                                                     <  2:538050/143‑1 (MQ=255)

             tttGGGATTCTGCGTTTAAAGTAGATAATTCGCCATTTCGTGCTACTTACGATTGTTTACGCAAGACGGCCAAAATATTTATATTTGGTTCTTTTATTGTTTTCTTGACACTAACTAGCCAGCTAAAATCAAAAAAAGAGAgt                                                                                                                                   <  1:1271276/143‑1 (MQ=255)

                 gTATTCTGCGTTTAAAGTAGATAATTCGCCATTTCGTGCTACTTACCATAGTTATTTAAATACTGCCAAAATATTTATATTTGGTTCTTTTATTGTTTTCTTGACACTAACTAGCCAGCTAAAATCAAAAAAAGAGAGTGtat                                                                                                                               <  1:32715/143‑1 (MQ=255)

                 gTATTCTGCGTTTAAAGTAGATAATTCGCCATTTCGTGCTACTTACCATAGTTATTTAAATACTGCCAAAATATTTATATTTGGTTCTTTTATTGTTTTCTGGACACTAACTAGCCAGAAAAAAAAAAAAAAAGAGAGTGtat                                                                                                                               <  2:95888/143‑1 (MQ=255)

                    ttCTGCGTTTAAAGTAGATAATTCGCCATTTCGTGCTACTTACCATAGTTATTTAGATACTGCCAAAATATTTATATTTGGTTCTTTTATTGTTTTCTTGACACTAACTAGCCAGCTAAAATCAAAAAAAGAGAGTGtattat                                                                                                                            >  1:734477/1‑143 (MQ=255)

                     tCTGCGTTTAAAGTAGATAATTCGCCATTTCGTGCTACTTACCATAGTTATTTAAATACTGCCAAAATATTTATATTTGGTTCTTTTATTGTTTTCTTGACACTAACTAGCCAGCTAAAATCAAAAAAAGAGAGTGTATtata                                                                                                                           <  1:953709/143‑1 (MQ=255)

                      cTGCGTTTAAAGTAGATAATTCGCCATTTCGTGCTACTTACCATAGTTATTTAAATACTGCCAAAATATTTATATTTGGTTCTTTTATTGTTTTCTTGACACTAACTAGCCAGCTAAAATCAAAAAAAGAGAGTGTATTATac                                                                                                                          >  1:412163/1‑143 (MQ=255)

                         cGTTTAAAGTAGATAATTCGCCATTTCGTGCTACTTACCATAGTTATTTAAATACTGCCAAAATATTTAGATTTGGTTCTTTTATTGTTTTCTTGACACTAACTAGCTAGCTAAAATAAAAAAAAGAGAGGGTATTATACACt                                                                                                                       >  1:494885/1‑143 (MQ=255)

                          gTTTAAAGTAGATAATTCGCCATTTCGTGCTACTTACCATAGTTATTTAAATACTGCCAAAATATTTATATTTGGTTCTTTTATTGTTTTCTTGACACTACCTACGCATCTAAAATCAAAAAAAGAGAGTGTATTATACACtt                                                                                                                      >  1:867555/1‑143 (MQ=255)

                           tttAAAGTAGATAATTCGCCATTTCGTGCTACTTACCATAGTTATTTAAATACTGCCAAAATATTTATATTTGGTTCTTTTATTGTTTTCTTGACACTAACTAGCCAGCTAAAATCAAAAAAAGAGAGTGTATTATACACttt                                                                                                                     >  1:846038/1‑143 (MQ=255)

                           tttAAAGTAGATAATTCGCCATTTCGTGCTACTTACCATAGTTATTTAAATACTGCCAAAATATTTATATTTGGTTCTTTTATTGTTTTCTTGACACTAACTAGCCAGCTAAAATCAAAAAAAGAGAGTGTATTATACACttt                                                                                                                     <  1:116548/143‑1 (MQ=255)

                           tttAAAGTAGATAATTCGCCATTTCGTGCTACTTACCATAGTTATTTAAATACTGCCAAAATATTTATATTTGGTTCTTTTATTGTTTTCTTGACACTAACTAGCCAGCTAAAATCAAAAAAAGAGAGTGTATTATACACttt                                                                                                                     >  1:1279559/1‑143 (MQ=255)

                           tttAAAGTAGATAATTCGCCATTTCGTGCTACTTACCATAGTTATTTAAATACTGACAACACATGGATAATTGGTTCTTTTCTTGTTTTCTTGACACTAACTAGCCAGCTAAAATCAAAAAAAGAGAGTGTATTATACACttt                                                                                                                     >  1:538305/1‑143 (MQ=255)

                             tAAAGTAGATAATTCGCCATTTCGTGCTACTTACCATAGTTATTTAAATACTGCCAAAATATTTATATTTGGTTCTTTTATTGTTTTCTTGACACTAACTAGCCAGCTAAAATCAAAAAAAGAGAGTGTATTATACACTTTGt                                                                                                                   <  2:807218/143‑1 (MQ=255)

                             tAAAGTAGATAATTCGCAAATTCGTGCTACTTACCATAGTTATTTAAATACTGCCAAAATATTTATATATGGTTATTTTATTGTTTACTTGAAACTAACTAGCAAGCTAAAAAAAAAAAAAGAGAGTGTATTATACACTTTGt                                                                                                                   <  2:690355/143‑1 (MQ=255)

                               tagcaGATAATTCGCCATTTCGTGCTACTTACCATAGTTATTTAAATACTGACAAAATATTTATATTTGGTGCTTTTATTGTTTTCTTGACACAAAAGAACAAGCAAAAATCAAAAAAAGAGAGTGTATTATACACTTTGTAt                                                                                                                 <  2:1044754/139‑1 (MQ=255)

                                  tAGATAATTCGCCATTTCGTGCTACTTACCATAGTTATTTAAATACTGCCAAAATATTTATATTTGGTTCTTTTATTGTTTTCTTGACACTAACTAGCCAGCTAAAATCAAAAAAAGAGAGTGTATTATACACTTTGTATtct                                                                                                              >  1:842228/1‑143 (MQ=255)

                                   agctAATTCGCCATTTTGTGCTACTTACCATAGTTATTTAAATACTGCCAAAATATTTATATTTGGTTCTTTTATTGTTTTCTTGACACTAACTAGCCAGCTAAAATCAAAAAAAGAGAGTGTATTATACACTTTGTATtctc                                                                                                             <  1:985569/140‑1 (MQ=255)

                                   aGATAATTCGCCATTTCGTGCTACTTACCATAGTTATTTAAATACTGCCAAAATATTTATATTTGGTTCTTTTATTGTTTTCTTGACACTAACTAGCCAGCTAAAATCAAAAAAAGAGAGTGTATTATACACTGTGTATtctc                                                                                                             >  2:945429/1‑143 (MQ=255)

                                    gATAATTCGCCATTTCGTGCTACTTACCATAGTTATTTAAATACTGCCAAAATATTTATATTTGGTTCTTTTATTGTTTTCTTGACACTAACTAGCCAGCTAAAATCAAAAAAAGAGAGTGTATTATACACTTTGTATTctct                                                                                                            <  2:255091/143‑1 (MQ=255)

                                         ttCGCCATTTCGTGCTACTTACCATAGTTATTTAAATACTGCCAAAATATTTATATTTGGTTCTTTTATTGTTTTCTTGACACTAACTAGCCAGCTAAAATCAAAAAAAGAGAGTGTATTATACACTTTGTATTCTCTGTCAt                                                                                                       >  1:176529/1‑143 (MQ=255)

                                           cgcgATTTCGTGCTACTAACAATAGTGATTTAAATACTAACAAAATAAATATATTTGATTCTAATAAAGATTTCATGACACTAACTAGCCAAAAAAAAAAAAAAAAAGAGAGTGTATTATACACTTTGTATTCTCTGTCAttt                                                                                                     <  2:122762/143‑1 (MQ=255)

                                           cGCCATTTGGGGCTACGTACCATAGTTATTTAAATACTGCCAAAATATTTATATTTGGTTCTTTTATTGTTTTCTTGACACTAACTAGCCAGCTAAAATCAAAAAAAGAGAGTGTATTATACACTTTGTATTCTCTGTCAttt                                                                                                     <  2:496852/143‑1 (MQ=255)

                                           cGCCATTTCGTGCTACTTACCATAGTTATTTAAATACTGCCAAAATATTTATATTTGGTTCTTTTATTGTTTTCTTGACACTAACTAGCCAGCTAAAATCAAAAAAAGAGAGTGTATTATACACTTTGTATTCTCTGTCAttt                                                                                                     <  1:719893/143‑1 (MQ=255)

                                            gCCATTTCGTGCTACTTACCATAGTTATTTAAATACTGCCAAAATATTTATATTTGGTTCTTTTATTGTTTTCTTGACACTAACTAGCCAGCTAAAATCAAAAAAAGAGAGTGTATTATACACTTTGTATTCTCTGTCATTTc                                                                                                    >  1:961537/1‑143 (MQ=255)

                                               attgtgTGGTATTTAGCATTGATATTTAAATACTGCCAAAATATTTATATTTTGTTCTTTTAGTGTTTTCTTGACACTAACTAGCCAGCTAAAATCAAAAAAAGAGAGTGTATTATACACTTTGTATTCTCTGTCATTTCTaa                                                                                                 <  1:641107/138‑1 (MQ=255)

                                               aTTTCGTGCTACTTACCATAGTTATTTAAATACTGCCAAAATATTTATATTGGGTTCTTTTATTGTTTCCTTGACACTAACTAGCCAGCTAAAATCAAAAAAAGAGAGTGTATTATACACTTTGTATTCTCTGTCATTTCTaa                                                                                                 <  1:653964/143‑1 (MQ=255)

                                                tttCGTGCTACTTACCATAGTTATTTAAATACTGCCAAAATATTTATATTTGGTTCTTTTATTGTTTTCTTGACACTAACTAGCCAGCTAAAATCAAAAAAAGAGAGTGTATTATACACTTTGTATTCTCTGTCATTTCTAAt                                                                                                <  1:368663/143‑1 (MQ=255)

                                                tttCGTGCTACTTACCATAGTTATTTAAATACTGCCAAAATATTTATATTTGGTTCTTTTATTGTTTTCTTGACACTAACTAGCCAGCTAAAATCAAAAAAAGAGAGTGTATTATACACTTTGTATTCTCGGTCGTTTCTAAt                                                                                                >  2:716373/1‑143 (MQ=255)

                                                  tCGTGCTACTTACCATAGTTATTTAAATACTGCCGTTATAGTTATATTTGGTTCTTTTATTGTTTTCTTGACACTAACTAGCCAGCTAAAATCAAAAAAAGAGAGTGTATTATACACTTTGTATTCTCTGTCATTTCTAATTg                                                                                              <  2:900088/143‑1 (MQ=255)

                                                  tCGTGATAATTACAATAGTTAGTGAAATACTGCCAAAATATTTATATTTGGTTCTTTTATTGTTTTATGGCCACTATCGAGCCAAAAAAAAAAAAAAAAAGAGAGTGTATTATACACTTTGTATTCTCTGTCATTTCTAATTg                                                                                              <  2:158144/143‑1 (MQ=255)

                                                     tGCTACTTACCATAGTTATTTAAATACTGCCAAAATATTTATATTTGGTTCTTTTATTGTTTTCTTGACACTAACTAGCCAGCTAAAATCAAAAAAAGAGAGTGTATTATACACTTTGTATTCTCTGTCATTTCTAATTGCTg                                                                                           <  2:1058400/143‑1 (MQ=255)

                                                      gCTACTTACCATAGTTATTTAAATACTGCCAAAATATTTATATTTGGTTCTTTTATTGTTTTCTTGACACTAACTAGCCAGCTAAAATCAAAAAAAGAGAGTGTATTATACACTTTGTATTCTCTGTCATTTCTAATTGCTgg                                                                                          >  1:1141762/1‑143 (MQ=255)

                                                       cTACTTACCATAGTTATTTAAATACTGCCAAAATATTTATATTTGGTTCTTTTATTGTTTTCTTGACACTAACTAGCCAGCTAAAATCAAAAAAAGAGAGTGTATTATACACTTTGTATTCTCTGTCATTTCTAATTGCTGGa                                                                                         <  1:256378/143‑1 (MQ=255)

                                                          cTTACCATAGTTATTTAAATACTGCCAAAATATTTATATTTGGTTCTTTTATTGTTTTCTTGACACTAACTAGCCAGCTAAAATCAAAAAAAGAGAGTGTATTATACACTTTGTATTCTCTGTCATTTCTAATTGCTGGatat                                                                                      <  2:1139604/143‑1 (MQ=255)

                                                            tACCATAGTTATTTAAATACTGCCAAAATATTTATATTTGGTTCTTTTATTGTTTTCTTGACACTAACTAGCCAGCTAAAATCAAAAAAAGAGAGTGTATTATACACTTTGTATTCTCTATCATTTCTAATTGCTCGATATGc                                                                                    >  2:371468/1‑143 (MQ=255)

                                                             aCCATAGTTATTTAAATACTGCCAAAATATTTATATTTGGTTCTTTTATTGTTTTCTTGACACTAACTAGCCAGCTAAAATCAAAAAAAGAGAGTGTATTATACACTTTGTATTCTCTGTCATTTCTAATTGCTGGATATGCa                                                                                   <  1:352142/143‑1 (MQ=255)

                                                                aTAGTTATTTAAATACTGCCAAAATATTTATATTTGGTTCTTTTATTGTTTTCTTGACACTAACTAGCCAGCTAAAATCAAAAAAAGAGAGTGTATTATACACTTTGTATTCTCTGTCATTTCTAATTGCTGGATATGCAATg                                                                                >  1:522647/1‑143 (MQ=255)

                                                                  aGTTATTTAAATACTGCCAAAATATTTATATTTGGTTCTTTTATTGTTTTCTTGACACTAACTAGCCAGCTAAAATCAAAAAAAGAGCGTGTCTTATACACTTTGTATTCTCTGTCATTTCTAATTGCTGGATATGCAATGta                                                                              >  1:12255/1‑143 (MQ=255)

                                                                    ttATTTAAATACTGCCAAAATATTTATATTTGGTTCTTTTATTGTTTTCTTGACACTAACTAGCCAGCTAAATTCAATATAACAGAGCGTATTCTACACTTTGTCTTCTCTGTCACTTCTCATTGCTGGCTCTGCAATGtata                                                                            >  2:12559/1‑143 (MQ=255)

                                                                       tttAAATACTGCCAAAATATTTATATTTGGTTCTTTTATTGTTTTCTTGACACTAATTAGCCAGCTAAAATCAAAAAAAGAGAGTGTATTATACACTTTGTATTCTCTGTCATTTCTAATTGCTGGATATGCAATGTATATTa                                                                         <  2:656016/143‑1 (MQ=255)

                                                                        ttAAATACTGCCAAAATATTTATATTTGGTTCTTTTATTGTTTTCTTGACACTAACTAGCCAGCTAAAATCAAAAAAAGGGAGTGTATTATACACTTTGTATTCTCTGTCATTTCTAATTGCTGGATATGGAATGTATATTaa                                                                        >  1:871355/1‑143 (MQ=255)

                                                                        ttAAATACTGCCAAAATATTTATATTTGGTTCTTTTATTGTTTTCTTGACACTAACTAGCCAGCTAAAATCAAAAAAAGAGAGTGTATTATACACTTTGTATTCTCTGTCATTTCTAATTGCTGGATAGTCAATGTATATTaa                                                                        >  2:376297/1‑143 (MQ=255)

                                                                          aaaTACTGACAAAATATTTAGATTTGGTTCTTTTATTGTTTTCTTGACACTAACTAGCCAGCTAAAATCAAAAAAAGAGAGTGTATTATACACTTTGTATTCTCTGTCATTTCTAATTGCTGGATATGCAATGTATATTAATa                                                                      <  1:282989/143‑1 (MQ=255)

                                                                             tACTGCCAAAATATTTATATTTGGTTCTTTTATTGTTTTCTTGACACTAACTAGCCAGCTAAAATCAAAAAAAGAGAGTGTATTATACACTTTGTATTCTCTGTCATTTCTAATTGCTGGATATGCAATGTATATTAATAGCa                                                                   >  1:534281/1‑143 (MQ=255)

                                                                              aCTGCCAAAATATTTATATTTGGTTCTTTTATTGTTTTCTTGACACTAACTAGCCAGCTAAAATCAAAAAAAGAGAGTGTATTATACACTTTGTATTCTCTGTCATTTCTAATTGCTGGATATGCAATGTATATTAATAGCAt                                                                  <  1:524345/143‑1 (MQ=255)

                                                                               cTGCCAAATTATTTATATTTGGTTCTTTTATTGTTTTCTTGACACTAACTAGCCAGCTAAAATCAAAAAAAGAGAGTGTATTATACACTTTGTATTCTCTGTCATTTCTAATTGCTGGATATGCAATGTATATTAATAGCAtt                                                                 <  2:508567/143‑1 (MQ=255)

                                                                               cTGCCAAAATATTTATATTTGGTTCTTTTATTGTTTTCTTGACACTAACTAGCCAGCTAAAATCAAAAAAAGAGAGTGTATTATACACTTTGTATTCTCTGTCATTTCTAATTGCTGGATATGCAATGTATATTAATAGCAtt                                                                 <  1:684008/143‑1 (MQ=255)

                                                                                tGCCAAAATATTTATATTTGGTTCTTTTATTGTTTTCTTGACACTAACTAGCCAGCTAAAATCAAAAAAAGAGAGTGTATTATACACTTTGTATTCTCTGTCATTTCTAATTGCTGGATATGCAATGTATATTAATAGCATTc                                                                >  2:33680/1‑143 (MQ=255)

                                                                                tGCCAAAATAATTAAATTTGGTTCTTTTATTGTTTTCTTGACACTAACTAGCCAGCTAAAATCAAAAAAAGAGAGTGTATTATACACTTTGTATTCTCTGTCATTTCTAATTGCTGGATATGCAATGTATATTAATAGCATTc                                                                <  1:275364/143‑1 (MQ=255)

                                                                                 gCCAAAATATTTATATTTGGTTCTTTTATTGTTTTCTTGACACTAACTAGCCAGCTAAAATCAAAAAAAGAGAGTGTATTATACACTTTGTATTCTCTGTCATTTCTAATTGCTGGATATGCAATGTATATTCATAGCCTTCa                                                               >  2:1079640/1‑143 (MQ=255)

                                                                                 gCCAAAATAATTATAATAGATTATTATAATGTTTAATTAACAATAAATAACCAAATAAAAACAAAAAAAGAGAGTGTATTATACACTTTGTATTCTCTGTCATTTCTAATTGCTGGATATGCAATGTATATTAATAGCATTCa                                                               <  2:1281942/143‑1 (MQ=255)

                                                                                    aaaaTATTTATATTTGGTTCTTTTATTGTTTTCTTGACACTAACTAGCCAGCTAAAATCAAAAAAAGAGAGTGTATTATACACTTTGTATTCTCTGTCATTTCTAATTGCTGGATATGCCACGTATATTAATAGCATTCATGa                                                            >  2:309298/1‑143 (MQ=255)

                                                                                    aaaaTATTTATATTTGGTTCTTTTATTGTTTTCTTGACACTAACTAGCCAGCTAAAATCAAAAAAAGAGAGTGTATTATACACTTTGTATTCTCTGTCATTTCTAATTGCTGGATATGCAATGTATATTAATAtcattcatta                                                            >  2:417958/1‑141 (MQ=255)

                                                                                    aaaaTATTTATATTTGGTTCTTTTATTGTTTTCTTGACACTAACTAGCCAGCTAAAATCAAAAAAAGAGAGTGTATTATACACTTTGTATTCTCTGTCATTTCTAATTGCTGGATATGCAATGTATATTAATAGCTTTCTTGa                                                            >  2:157423/1‑143 (MQ=255)

                                                                                       atatttatatttGGTTCTTTTATTGTTTTCTTGACACTAACTAGCCAGCTAAAATCAAAAAAAGAGAGTGTATTATACACTTTGTATTCTCTGTCATTTCTAATTGCTGGATATGCAATGTATATTAATAGCATTCATGaaaa                                                         <  1:188329/143‑1 (MQ=255)

                                                                                       atattgatatttGGTTCTTTTATTGTTTTCTTTACTCTAACTAGCCAGCTAAAATCAAAAAAAGAGAGTGTATTATACACTTTGTATTCTCTGTCATTTCTAATTGCTGGATATGCAATGTATATTAATAGCATTCATGaaaa                                                         <  1:1206818/143‑1 (MQ=255)

                                                                                           ttatatttGGTTCTTTTATTGTTTTCTTGACACTAACTAGCCAGCTAAAATCAAAAAAAGAGAGTGTATTATACACTTTGTATTCTCTGTCATTTCTAATTGCTGGATATGCAATGTATATTAATAGCATTCATGAAAATGAc                                                     <  1:21699/143‑1 (MQ=255)

                                                                                           ttatatttGGTTCTTTTATTGTTTTCTTGACACTAACTAGCCAGCTAAAATCAAAAAAAGAGAGTGTATTATACACTTTGTATTCTCTGTCATTTCTAATTGCTGGATATGCAATGTATATTAATAGCATTCATGAAAATGAc                                                     >  1:614993/1‑143 (MQ=255)

                                                                                           ttatatttGGTTCTTTTATTGTTTTCTTGACACTAACTAGCCAGCTAAAATCAAAAAAAGAGAGTGTATTATACACTTTGTATTCTCTGTCATTTCTAATTGCTGGATATGCAATGTATATTAATAGCATTCATGAAAATGAc                                                     >  1:1110414/1‑143 (MQ=255)

                                                                                            tatatttGGTTCTTTTATTGTTTTCTTGACACTAACTAGCCAGCTAAAATCAAAAAAAGAGAGTGTATTATACACTTTGTATTCTCTGTCATTTCTAATTGCTGGATATGCAATGTATCTTAATAGCATTCATCAAAATGAcc                                                    >  2:336020/1‑143 (MQ=255)

                                                                                            tatatttGGTTCTTTTATTGTTTTCTTGACACTAACTAGCCAGCTAAAATCAAAAAAAGAGAGTGTATTATACACTTTGTATTCTCTGTCATTTCTAATTGCTGGATATGCAATGTATATTAATAGCATTCATGAAAATGAcc                                                    >  1:1168963/1‑143 (MQ=255)

                                                                                             atatttGGTTCTTTTATTGTTTTCTTGACACTAACTAGCCAGCTAAAATCAAAAAAAGAGAGTGTATTATACACTTTGTATTCTCTGTCATTTCTAATTGCTGGATATGCAATGTATATTAATAGCATTCATGAAAATGACCg                                                   <  2:1277300/143‑1 (MQ=255)

                                                                                             atatttGGTTCTTTTATTGTTTTCTTGACACTAACTAGCCAGCTAAAATCAAAAAAAGAGAGTGTATTATACACTTTGTATTCTCTGTCATTTCTAATTGCTGGATATGCAATGTATATTAATAGCATTCATGAAAATGACCg                                                   <  1:736487/143‑1 (MQ=255)

                                                                                                 ttGGTTCTTTTATTGTTTTCTTGACACTAACTAGCCAGCTAAAATCAAAAAAAGAGAGTGTATTATACACTTTGTATTCTCTGTCATTTCTAATTGCTGGATATGCAATGTATTTTAATAGCATTAATGAAAATGACCGCAtt                                               >  2:411832/1‑143 (MQ=255)

                                                                                                      tcattaaTTGTTTTATTGACACTAACTAGCAAAAAAAAAAAAAAAAAAGAGAGTGTATTATACACTTTGTATTCTCTGTCATTTCTAATTGCTGGATATGCAATGTATATTAATAGCATTCATGAAAATGACCGCATTTCttt                                          <  2:1056503/137‑1 (MQ=255)

                                                                                                      tCTTTTATTGTTTTCTTGACACTAACTAGCCAGCTAAAATCAAAAAAAGAGAGTGTATTATACACTTTGTATTCTCTGTCATTTCTAATTGCTGGATATGCAATGTATATTAATAGCATTCATGAAAATGACCGCATTTCttt                                          <  1:492357/143‑1 (MQ=255)

                                                                                                       gttttATTGTTTTCTTGACACTAACTAGCCAGATAAAAACAAAAAAAGAGAGTGTATTATACACTTTGTATTCTCTGTCATTTCTAATTGCTGGATATGCAATGTGTCTTCCTAGCATTCATGAAAATGACCGCATTTCtttt                                         <  2:1254071/142‑1 (MQ=255)

                                                                                                       cTTTTATTGTTTTCTTAACACTAACTAGCCAGCTAAAATCAAAAAAAGAGAGTGTATTATACACTTTGTATTCTCTGTCATTTCTAATTGCTGGATATGCAATGTATATTAATAGCATTCATGAAAATGACCGCATTTCtttt                                         <  2:1161554/143‑1 (MQ=255)

                                                                                                       cTTTTAGTGTTTTCGTGACACTAACTAGCCAGCTAAAATCAAAAAAAGAGAGTGTATTATACACTTTGTATTCTCTGTCATTTCTAATTGCTGGATATGCAATGTATATTAATAGCATTCATGAAAATGACCGCATTTCtttt                                         <  2:1259346/143‑1 (MQ=255)

                                                                                                        tttaATTGTTTTCTTGACACTAACTAGCCAGCTAAAATCAAAAAAAGAGAGTGTATTATACACTTTGTATTCTCTGTCATTTCTAATTGCTGGATATGCAATGTATATTAATAGCATTCATGAAAATGACCGCATTTCTTTTg                                        <  2:342702/143‑1 (MQ=255)

                                                                                                         tttATTGTTTTCTTGACACTAACTAGCCAGCTAAAATCAAAAAAAGAGAGTGTATTATACACTTTGTATTCTCTGTCATTTCTAATTGCTTGATATGCATTATATAAGATTAGTATTCATGAAAATGACCGCATTTCtttttg                                       >  2:901260/1‑141 (MQ=255)

                                                                                                         tttATTGTTTTCTTGACACTAACTAGCCAGCTAAAATCAAAAAAAGAGAGTGTATTATACACTTTGTATTCTCTGTCATTTCTAATTGCTGGATATGCAATGTATATTAATAGCATTCATGAAAATGACCGCATTTCTTCTgg                                       >  1:1242481/1‑143 (MQ=255)

                                                                                                                ttttCTTGACACTAACTAGCCAGCTAAAATCAAAAAAAGAGAGTGTATTATACACTTTGTATTCTCTGTCATTTCTAATTGCTGGATATGCAATGTATATTAATAGCATTCATGAAAATGACCGCATTTCTTTTGGTGTAGGa                                >  1:555881/1‑143 (MQ=255)

                                                                                                                  ttCTTGACACTAACTAGCCAGCTAAAATCAAAAAAAGAGAGTGTATTATACACTTTGTATTCTCTGTCATTTCTAATTGCTGGATATGCAATGTATATTAATAGCATTCATGAAAATGACCGCATTTCTTTGGGTGTAGGAAc                              <  1:452344/143‑1 (MQ=255)

                                                                                                                   tCTTGACACTAACTAGCCAGCTAAAATCAAAAAAAGAGAGTGTATTATACACTTTGTATTCTCTGTCATTTCTAATTGCTGGATATGCAATGTATATACATAACGCAAATTTAAATGACCGCATTTCTTTTGGTGTAGGAACg                             >  2:1239521/1‑143 (MQ=255)

                                                                                                                     ttGACACTAACTAGCCAGCTAAAATCAAAAAAAGAGAGTGTATTATACACTTTGTATTCTCTGTCATTTCTAATTGCTGGATATGCAATGTATATTAATAGCATTCATGAAAATGACCGCATTTCTTTTGGTGTAGGAACGGc                           <  1:957934/143‑1 (MQ=255)

                                                                                                                       gACACTAACTAGCCAGCTAAAATCAAAAAAAGAGAGTGTATTATACACTTTGTATTCTCTGTCATTTCTAATTGCTGGATATGCAATGTATATTAATAGCATTCATGAAAATGACCGCATTTCTTTTGGTGTAGGAACGGCaa                         >  1:689997/1‑143 (MQ=255)

                                                                                                                          actaactaGCCAGCTAAAATCAAAAAAAGAGAGTGTATTATACACTTTGTATTCTCTGTCATTTCTAATTGCTGGATATGCAATGTATATTAATAGCATTCATGAAAATGACCGCATTTCTTTTGGTGTAGGAACGGCAACAg                      >  1:1058415/1‑143 (MQ=255)

                                                                                                                          actaactaGCCAGCTAAAATCAAAAAAAGAGAGTGTATTATACACTTTGTATTCTCTGTCATTTCTAATTGCTGGATATGCAATGGATATTAATAGCATTCATGAAAATGACCGCATTTCTTTTGGTGTAGGAACGGCAACAg                      >  1:1072335/1‑143 (MQ=255)

                                                                                                                          acgtactaGCCAGCTAAAATCAAAAAAAGAGTGTGTATTATACTCTTTGTATTCTCTGTCATTTCTAATTGCTGGATATGCAATGTATATTAATAGCATTCATGAAAATGACCGCATTTCTTTTGGTGTAGGAACGGCAACAg                      <  2:1104663/139‑1 (MQ=255)

                                                                                                                                 accgaGCTACAATCAAAAAAAGAGAGTGTATTATACTCTTTGTATCCTCTGTCATTTCTAATTGCTGGATATGCACTGTATATTAATAGCATTCATGAAAATGACCGCATTTCTTTTGGTGTAGGAACGGCAACAGGagcagc               <  2:402973/139‑1 (MQ=255)

                                                                                                                                   ccAGCTAAAATCAAAAAAAGAGAGTGTATTATACACTTTGTATTCTCTGTCATTTCTAATTGCAGGATATGCAAGGTATATTAATAGCATTCATGAAAATGAGCGCATTTGTTTTGGTGTAGGAACGGCAAGAGGAGCAGCat             >  1:408883/1‑143 (MQ=255)

                                                                                                                                     aGCTAAAATCAAAAAAAGAGAGTGTATTATACACTTTGTATTCTCTGTCATTTCTAATTGCTGGATATGCAATGTATATTAATAGCATTCATGAAAATGACAGCATTTCTTTTGGTGTAGGAACGGCAACAGGAGCAGCatat           >  2:1195908/1‑143 (MQ=255)

                                                                                                                                        tAAAATCAAAAAAAGAGAGTGTATTATACACTTTGTATTCTCTGTCATTTCTAATTGCTGGATATGCAATGTATATTAATAGCATTCATGAAAATGACCGCATTTCTTTTGGTGTAGGAACGGCAACAGGAGCAGCATATTca        <  1:60553/143‑1 (MQ=255)

                                                                                                                                          aaaTCAAAAAAAGCGAGTGTATTATACACTTTGTATTCTCTGTCATTTCTAATTGCTGGATATGCAATGTATGTTAATAGCATTCATGAAAATGACCGCATTTCTTTTGGTGTAGGAACGGCAACAGGAGCAGTATGTTcatc      >  1:35352/1‑141 (MQ=255)

                                                                                                                                            accAAAAAAAGAGAGTGTATTATACACTTTGTATTCTCTGTCATTTCTAATTGCTGGATATGCAATGTATATTAATAGCATTCATGAAAATGACCGCATTTCTTTTGGTGTAGGAACGGCAACAGGAGCAGCATATTcaacaa    <  2:87483/141‑1 (MQ=255)

                                                                                                                                             tCAAAAAAAGAGAGTGTATTATACACTTTGTATTCTCTGTCATTTCTAATTGCTGGATATGCAATGTATATTAATAGCATTCATGAAAATGACCGCATTTCTTTTGGTGTAGGAACGGCAACAGGAGCAGCATATTCAACAAt   >  2:609085/1‑143 (MQ=255)

                                                                                                                                             tCAAAAAAAGAGAGTGTATTATACACTTTGTATTCTCTGTCATTTCTAATTGCTGGATATGCAATGTATATTAATAGCATTCATGAAAATGACCGCATTTCTTTTGGTGTAGGAACGGCAACAGGAGCAGCATATTCAACAAt   >  1:759459/1‑143 (MQ=255)

                                                                                                                                             tCAAAAAAAGAGAGTGTATTATACACTTTGTATTCTCTGTCATTTCTAATTGCTGGATATGCAATGTATATTAATAGCATTCATGAAAATGACCGCATTTCTTTTGGTGTAGGAACGGCAACAGGAGCAGCATATTCAACAAt   <  2:534281/143‑1 (MQ=255)

                                                                                                                                              cAAAAAAAGAGAGTGTATTATACACTTTGTATTCTCTGTCATTTCTAATTGCTGGATATGCAATGTATATTAATAGCATTCATGAAAATGACCGCATTTCTTTTGGTGTAGGAACGGCAACAGGAGCAGCATATTCAACAATg  <  2:78458/143‑1 (MQ=255)

TTGCTTGATTTAATTTGGTATTCTGCGTTTAAAGTAGATAATTCGCCATTTCGTGCTACTTACCATAGTTATTTAAATACTGCCAAAATATTTATATTTGGTTCTTTTATTGTTTTCTTGACACTAACTAGCCAGCTAAAATCAAAAAAAGAGAGTGTATTATACACTTTGTATTCTCTGTCATTTCTAATTGCTGGATATGCAATGTATATTAATAGCATTCATGAAAATGACCGCATTTCTTTTGGTGTAGGAACGGCAACAGGAGCAGCATATTCAACAATG  >  NC_000913/3797194‑3797478

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 13 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`