BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	3,839,567	G→C	5.2%	L143V (CTT→GTT)	nlpA ←	lipoprotein‑28

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,839,567	0	G	C	5.2%	325.2 / 10.2	116	L143V (CTT→GTT)	nlpA	lipoprotein‑28
Reads supporting (aligned to +/- strand): ref base G (55/54); new base C (4/2); total (59/57)
Fisher's exact test for biased strand distribution p-value = 6.80e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.22e-01

Only 100 of 123 total aligned reads displayed.

CGCGGGAGCTGCGCCCCCTCGAGTTCCATAATCTGCAAATGACGAGGATTATCGGTTATATCCAGCGCGGTAGGTAATAACCCTTTTCCCTCTTTCAGAGTAATTAGCTTCTCTTTTTGCAACAGTAAAAGTGCGCGACCAAGGTTGGTCGGGTCGTTGGGGATCGCCACTGTCGCGCCTTCTTTTATTTGCGCCACGGTTTTGATTTTTTTGGAATAACCCGCCAT‑AGGGAAGACAAACGTATTTCCCACCGCTACCAGTTTATAACCATGCGCCTGATTATCT  >  NC_000913/3839425‑3839709

cgcgGGAGCTGCGCCCCCTCGAGTTCCATAATCTGCAAATGACGAGGATTATCGGTTATATCCAGCGCGGTAGGTAATAACCCTTTTCCCTCTTTCAGAGTAATTAGCTTCTCTTTTTGCAACAGTGAAAGTGCGCGACCAAg                                                                                                                                                 >  2:618932/1‑143 (MQ=255)

cgcgGGAGCTGCGCCCCCTCGAGTTCCATAATCTGCAAATGACGAGGATTATCGGTTATATCCAGCGCGGTAGGTAATAACCCTTTTCCCTCTTTCAGAGTAATTAGCTTCTCTTTTTGCAACAGTAAAAGTGCGCGACCAAg                                                                                                                                                 <  2:659474/143‑1 (MQ=255)

cgcgGGAGCTGCGCCCCCTCGAGTTCCATAATCTGCAAATGACGAGGATTATCGGTTATATCCAGCGCGGTAGGTAATAACCCTTTTCCCTCTTTCAGAGTAATTAGCTTCTCTTTTTGCAACAGTAAAAGTGCGCGACCAAg                                                                                                                                                 <  1:844736/143‑1 (MQ=255)

cgcgGCAGCTGCGCCCCCTCGAGTTCCATAATCTGCAAATGACGAGGATTATCGGTTATATCCAGCGCGGTAGGTAATAACCCTTTTCCCTCTTTCAGAGTAATTAGCTTCTCTTTTTGCAACAGTAAAAGTGCGCGACCAAg                                                                                                                                                 <  1:170886/143‑1 (MQ=255)

 gcgcGAGCTGCGCCCCCTCGAGTTCCATAATCTGCAAATGACGAGGATTATCGGTTATATCCAGCGCGGTAGGTAATAACCCTTTTCCCTCTTTCAGAGTAATTAGCTTCTCTTTTTGCAACAGTAAAAGTGCGCGACCAAgg                                                                                                                                                <  1:35130/143‑1 (MQ=255)

 gcgGGAGCTGCGCCCCCTCGAGTTCCATAATCTGCAAATGACGAGGATTATCGGTTATATCCAGCGCGGTAGGTAATAACCCTTTTCCCTCTTTCAGAGTAATTAGCTTCTCTTTTTGCAACAGTAAACGTGCGCGACCAAgg                                                                                                                                                >  1:378549/1‑143 (MQ=255)

  tgggACCGGCGCCGCCTGCAGTTCCATCTGCGGCAAATGACGAGCATTATCGGTTATCTCCAGCTCGGTAGGTAATAACCCTTTTCCCTCTTTCAGAGTAAGTAGCTTCTATTTTTGCAACAGTAAAAGTGCGCGACCAAGGt                                                                                                                                               <  2:1205939/142‑1 (MQ=255)

  cgGGAGCTGCGCCCCCTCGAGTTCCATAATCTGCAAATGACGAGGATTATCGGTTATATCCAGCGCGGTAGGTAATAACCCTTTTCCCTCTTTCAGAGTAATTAGCTTCTCTTTTTGCAACAGTAAAAGTGCGCGACCAAGGt                                                                                                                                               <  1:1073189/143‑1 (MQ=255)

  cgGGAGCTGCGCCCCCTCGAGTTCCATAATCCGGTAAGGACGGGGATTATCGGTTATATCCAGCGCGGTAGGTAATAACCCTTTTCCCTCTTTCAGAGTAATTAGCTTCTCTTTTTGCAACAGTAAAAGTGCGCGACCAAGGt                                                                                                                                               <  1:992185/143‑1 (MQ=255)

       ggtGCGCCCCCTGTGGTTCCATAGTCTGCAAATGACGAGGATTATCGGTTATATCCAGCGCGGTAGGTAATAACCCTTTTCCCTCTGTCAGAGTAATGAGCTTCTCTTTTTGCAACAGTAATAGTGCGCGACCAAGGTTggtc                                                                                                                                          <  2:158585/141‑1 (MQ=255)

        cTGCGCCCCCTCGAGTTCCATAATCTGCAAATGACGAGGATTATCGGTTATATCCAGCGCGGTAGGTAATAACCCTTTTCCCTCTTTCAGAGTAATTAGCTTCTCTTTTTGCAACAGTAAAAGTGCGCGACCAAGGTTggtcg                                                                                                                                         <  2:1235781/143‑1 (MQ=255)

        cTGCGCCCCCTCGAGTTCCATAATCTGCAAATGACGAGGATTATCGGTTATATCCAGCGCGGTAGGTAATAACCCTTTTCCCTCTTTCAGAGTAATTAGCTTCTCTTTTTGCAACAGTAAAAGTGCGCGACCAAGGTTggtcg                                                                                                                                         >  1:164816/1‑143 (MQ=255)

          gcgcCCCCTCGAGTTCCATAATCTGCAAATGACGAGGATTATCGGTTATATCCAGCGCGGTAGGTAATAACCCTTTTCCCTCTTTCAGAGTAATTAGCTTCTCTTTTTGCAACAGTAAAAGTGCGCGACCAAGGTTggccggg                                                                                                                                       >  1:195316/1‑143 (MQ=255)

           cgcCCCCTCGAGTTCCATAACCTGCAAATGACGATGATTATCACCTGTCTCTGGCTCTTTATATAATTATCCTTGGCCCTCTTTCAGAGTAATTAGCTTCTCTTTTTGCAACAGTAAAAGTGCGCGACCAACCTTggtcgcgc                                                                                                                                      <  2:269620/143‑4 (MQ=255)

              ccccTCGAGTTCCATAATCTGCAAATGACGAGGATTATCGGTTATATCCAGCGCGGTAGGTAATAACCCTTTTCCCTCTTTCAGAGTAATTAGCTTCTCTTTTTGCAACAGTAAAAGTGCGCGACCAACCTTggtcgcgcact                                                                                                                                   <  1:263630/143‑7 (MQ=255)

              ccccTCGAGTGCCATAATCTGCAAATGACGAGGATTAGCGGTTATATCCAGCGCGGTAGGTAATAACCCTTTTCCCTCTGTCAGAGTAATTAGCTTCGCTTTTTGCAACAGTAAAAGTGCGCGACCAAGGTTGGTCGGGTCGt                                                                                                                                   <  1:1216358/143‑1 (MQ=255)

               cccGCGTGTTCCATACTCTGCAAATTACGAGGATTTTTGGTTATTTCCAGCGCCGTAGGTAATAACCCTTTTCCCTCTTGCTGAGTTATTAGCTTCTCTTTTTGCAACAGTAAAAGTGCGCGACCAAGGTTGGTCGGGTCGtt                                                                                                                                  <  2:761467/143‑1 (MQ=255)

                ccTCGAGTTCCATAATCTGCAAATGACGAGGATTATCGGTTATATCCAGCGCGGTAGGTAATACCCCTTTTCCCCCTTCCAGAGCAAATCGCTTCTCTTTCTGCAACAGTCACAGTGCGCGACCACGGTTAGTCGAGTCGTTg                                                                                                                                 >  2:1186856/1‑143 (MQ=255)

                ccTCGAGTTCCATAATCTGCAAATGACGAGGATTATCGGTTATATCCAGCGCGGTAGGTAATAACCCTTTTCCCTCTTTCAGAGTAATTAGCTTCTCTTTTTGCAACAGTAAAAGTGCGCGACCAATGTTGGTCGGGTCGTTg                                                                                                                                 <  2:276092/143‑1 (MQ=255)

                 cTCGAGTTCCATAATCTGCAAATGACGAGGATTATCGGTTATATCCAGCGCGGTAGGTAATAACCCTTTTCGCTCTTTCAGAGTAATTAGCTTATCTTTTCGCAACAGTAAAAGTGCGCGACCAAGGTTGGCAGGGTCGTggg                                                                                                                                >  2:141352/1‑143 (MQ=255)

                  tCGAGTTCCATAATCTGTAAATGACGAGGATTATCGGTTATATCCAGCGCGGTAGGTAATAACCCTTTTCCCTCTTTCAGAGTAATTAGCTTCTCTTTTTGCAACAGTAAAAGTGCGCGACCAAGGTTGGTCGGGTCGTTggg                                                                                                                               >  1:625611/1‑143 (MQ=255)

                  tCGAGTGCCGTAGTGTGTAAATGACGAGGATTATCTGTCATATCCAGCGCGGTAGGTAATAACCCTTTTCCCTATTTCAGAGTAATTAGCTTCTCTTTTTGCAACAGTAAAAGTGCGCGACCAAGGTTGGTCGGGTCGTTggg                                                                                                                               <  2:414891/143‑1 (MQ=255)

                                acggagaTGACGAGGATTATCGGTTATATCCCGCGCGGTAGGTAATAGCCCTTTTCTCTCTTTCAGAGTAATTAGCTTCTCTTTTTGCAACAGTAAAAGTGCGCGACCAAGGTTGGTCGGGTCGTTGGGGATCGCCACTGTcg                                                                                                                 <  2:236317/137‑1 (MQ=255)

                                  gCAAATGACTAGGATTATCGGTTATATCCAGCGCGGTAGGTAATAACCCTTTTCCCTCTTTCAGAGTAATTAGCTTCTCTTTTTGCAACAGTAAAAGTGCGCGACCAAGGTTGGTCGGGTCGTTGGGGATCGCCACTGTcgcg                                                                                                               <  1:917493/143‑1 (MQ=255)

                                  ccAAATGACGAGCATTATCGGTTATATCCAGCGCGGTAGGTAATATCCCTTTTCCCTCTTGCAGAGTAATTAGCTTCTCTTTTTGCAACAGTAAAAGTGCGCGACCAAGGTTGGTCGGGTCGTTGGGGATCGCCACTGTcgcg                                                                                                               <  2:810749/142‑1 (MQ=255)

                                   caactcacgacgATTACCGGTTATATCCAGCGCGGTAGCTAATAACCCTGTTCCCTCTTTCAGAGTAATTAGCTTCTCTTTTTGCAACAGTAAAAGTGCGCGACCAAGGTTGGTCGGGTCGTTGGGGATCGCCACTGTCgcgc                                                                                                              <  2:896701/137‑1 (MQ=255)

                                     aaTGACGAGGATTATCGGTTATATCCAGCGCGGTAGGTAATAACCCTTTTCCCTCTTTCAGAGTAATTAGCTTCTCTTTTTGCAACAGTAAAAGTGCGCGACCAAGGTTCGTCGGGTCGTTGGGGATCGCCCCTGTCgcgcgt                                                                                                            >  2:1009536/1‑141 (MQ=255)

                                      aTGACGAGGATTATCGGTTATATCCAGCGCGGTAGGTAATAACCCTTTTCCCTCTTTCAGAGTAATTAGCTTCTCTTTTTGCAACAGTAAAAGTGCGCGACCAAGGTTGGTCGGGTCGTTGGGGATCGCCACTGTCGCGCctt                                                                                                           >  1:547510/1‑143 (MQ=255)

                                      aTGACGAGGATTATCGGTTATATCCAGCGCGGTAGGTAATAACCCTTTTCCCTCTTTCAGAGAAATTAGCTTCTCTTTTTGCAACAGTAAAAGTGCGCGACCAAGGTTGGTCGGGTCGTTGGGGATCGCCACTGTCGCGCctt                                                                                                           <  2:704940/143‑1 (MQ=255)

                                          cGATGATTATCGGTTATATCCAGCGCGGTAGGTAATAACCCTTTTCCCTCTTTCAGAGTAATTAGCTTCTCTTTTTGCAACAGTAAAAGTGCGCGACCAAGGTTGGTCGGGTCGTTGGGGATCGCCACTGTCGCGCCTTCttt                                                                                                       <  2:115398/143‑1 (MQ=255)

                                           gAGGATTATCGGTTATATCCAGCGCGGTAGGTAATAACCCTTTTCCCTCTTTCAGAGTAATTAGCTTCTCTTTTTGCAACAGTAAAAGTGCGCGACCAAGGTTGGTCGGGTCGGTGGGGATCGCCGCTGTCGCGCCGTCtttt                                                                                                      >  1:369561/1‑143 (MQ=255)

                                              gATTATCGGTTATATCCAGCGCGGTAGGTAATAACCCTTTTCCCTCTTTCAGAGTAATTAGCTTCTCTTTTTGCAACAGTAAAAGTGCGCGACCAAGGTTGGTCGGGTCGTTGGGGATCGCCACTGTCGCGCCTTCTTTTAtt                                                                                                   <  1:1012861/143‑1 (MQ=255)

                                              gATTATCGGTTATATCCAGCGCGGTAGGTAATAACCCTTTTCCCTCTTTCAGAGTAATTAGCTTCTCTTTTTGCAACAGTAAAAGTGCGCGACCAAGGTTGGTCGGGTCGTTGGGGATCGCCACTGTCGCGCCTTCTTTTAtt                                                                                                   <  1:1234079/143‑1 (MQ=255)

                                                ttttCGGTTATATCCAGCGCGGTAGGTAATAACCCTTTTCCCTCTTTCAGAGTAATTAGCTTCTCTTTTTGCAACAGTAAAAGTGCGCGACCAAGGTTGGTCGGGTCGTTGGGGATCGCCACTGTCGCGCCTTCTTTTATTTg                                                                                                 <  2:297057/140‑1 (MQ=255)

                                                ttATCGGTTATATCCAGCGCGGTAGGTAATAACCCTTTTCCCTCTTTCAGAGTAATTAGCTTCTCTTTTTGCAACAGTAAAAGTGCGCGACCAAGGTTGGTCGGGTCGTTGGGGATCGCCACTGTCGCGCCTTCTTTTATTTg                                                                                                 <  1:781896/143‑1 (MQ=255)

                                                 tATCGGTTATATCCAGCGCGGTAGGTAATAACCCTTTTCCCTCTTTCAGAGTAATTAGCTTCTCTTTTTGCAACAGTAAAAGTGCGCGACCAAGGTTGGTCGGGTCGTTGGGGATCGCCACTGTCGCGCCTTCTTTTATTTgc                                                                                                <  1:384892/143‑1 (MQ=255)

                                                 tATCGGTTATATCCAGCGCGGTAGGTAATAACCCTTTTCCCTCTTTCAGAGTAATTAGCTTCTCTTTTTGCAACAGTAAAAGTGCGCGACCAAGGTTGGTCGGGTCGTTGGGGATCGCCACTGTAGCGCCTTCTTTTATTTgc                                                                                                >  2:499346/1‑143 (MQ=255)

                                                   tCGGTTATATCCAGCGCGGTAGGTAATAACCCTTTTCCCTCTTTCAGAGTAATTAGCTTCTCTTTTTGCAACAGTAAAAGTGCGCGACCAAGGTTGGTCGGTGCGTTGCGGATCGCCACGGTCGCGCCTTCTTTTGTTTgtgc                                                                                              >  2:922722/1‑143 (MQ=255)

                                                   tCGGTTATATCCAGCGCGGTAGGTAATAACCCTTTTCCCTCTTTCAGAGTAATTAGCTTCTCTTTTTGCAACAGTAAAAGTGCGCGACCAAGGTTGGTCGGGTCGTTGGGGCTCGCCACTGTCGCGCCTTCTTTTGTTTgcgc                                                                                              >  1:306302/1‑143 (MQ=255)

                                                      gTTATATCCAGCGCGGTAGGTAATAACCCTTTTCCCTCTTTCAGAGTAATTAGCTTCTCTTTTTGCAACAGTAAAAGTGCGCGACCAAGGTTGGTCGGGTCGTTGGGGATCGCCACTGTCGCGCCTTCTTTTATTTGCGCCAc                                                                                           >  1:678042/1‑143 (MQ=255)

                                                       ttATATCCAGCGCGGTAGGTAATAACCCTTTTCCCTCTTTCAGAGTAATTAGCTTCTCTTTTTGCAACAGTAAAAGTGCGCGACCAAGGTTGGTCGGGTCGTTGGGGATCGCCACTGTCGCGCCTTCTTTTATTTGCGCCACg                                                                                          >  1:1256621/1‑143 (MQ=255)

                                                        tataTCCAGCGCGGTAGGTAATAACCCTTTTCCCTCTTTCAGAGTAATTAGCTTCTCTTTTTGCAACAGTAAAAGTGCGCGACCAAGGTTGGTCGGGTCGTTGGGGATCGCCACTGTCGCGCCTTCTTTTATTTGCGCCACgg                                                                                         >  2:662206/1‑143 (MQ=255)

                                                          ttcccAGCGCGGTAGGTAATGACCCTTTTTCCTCTTTCAGAGTAATTAGCTTCTCTTTTTGCAACAGTAAAAGTGCGCGACCAAGGTTGGTCGGGTCGTTGGGGATCGCCACTGTCGCGCCTTCTTTTATTTGCGCCACGGtt                                                                                       <  2:120755/140‑1 (MQ=255)

                                                           atCCAGCGCGGTAGGTAATAACCCTTTTCCCTCTTTCAGAGTAATTAGCTTCTCTTTTTGCAACACTAAAAGTGCGCGACCAAGGTTGGTCGGGTCGTTGGGGATCGCCACTGTCGCGCCTCCTTTTATTTGCCCCCCGGctt                                                                                      >  1:1234635/1‑143 (MQ=255)

                                                           atCCAGCGCGGTAGGTAATAACCCTTTTCCCTCTGTCAGCGTAAGTAGCTTCTCTTTTTGCAACAGTAAAAGTGCGCGACCAAGGTTGGTCGGGTCGTTGGGGATCGCCACTGTCGCGCCTTCTTTTATTTGCGCCACGGttt                                                                                      >  1:244229/1‑143 (MQ=255)

                                                             ctatcgcgGTAGGTAATACTCCCTTTCCCTCTTTCAGAGTAATTAGCTTCTCTTTTTGCAACAGTAACAGTTCGCGACTAAGGTTTGTCGGGTCGTTCGGGATCTCCACTGTCGCTCCTTCTTTTATTTGCGCCACGGTTTTg                                                                                    <  2:104772/139‑1 (MQ=255)

                                                              taGCGCGGTAGGTAATAACCCTTTTCCCTCTTTCAGAGTAATTAGCTTCTCTTTTTGCAACAGTAAAAGTGCGCGACCAAGGTTGGTCGGGTCGTTGGGGATCGCCACTGTCGCGCCTTCTTTTATTTGCGCCACGGTTTTgg                                                                                   >  1:760064/2‑142 (MQ=255)

                                                               aGCGCGGTAGGTAATAACCCTTTTCCCTCTTTCAGAGTAATTAGCTTCTCTTTTTGCAACAGTAAAAGTGCGCGACCAAGGTTGGTCGGGTCGTTGGGGATCGCCACTGTCGCGCCTTCTTTTCTTTGCGCCCCGGtttcgct                                                                                  >  1:112942/1‑139 (MQ=255)

                                                               aGCGCGGTAGGTAATAACCCTTTTCCCTCTTTCAGAGTAATTAGCTTCTCTTTTTGCAACAGTAAAAGTGCGCGACCAAGGTTGGTCGGGTCGTTGGGGATCGCCACTGTCGCGCCTTCTTTTATTTGCGCCACGGTTTTgtt                                                                                  >  1:784282/1‑141 (MQ=255)

                                                                   cgGTAGGTAATAACCCTTTTCCGTCTTTCCGCGTAATTAGCTTCTCTTTTTGCAACAGTAAAAGTGCGCGACCAAGGTTGGTCGGGTCGTTGGGGATCGCCACTGTCGCGCCTTCTTTTATTTGCGCCACGGTTTTGAttttt                                                                              <  2:1062041/143‑1 (MQ=255)

                                                                    ggtggttaataaCCCTTTTCCCTCTTTCAGAGTAATTAGCTTCTCTTTTTGCAACAGTAAAAGTGCGCGACCAAGGTTGGTCGGGTCGTTGGGGATCGCCACTGTCGCGCCTTCTTTTATTTGCGCCACGGTTTTGAtttttt                                                                             <  1:1086601/137‑1 (MQ=255)

                                                                     gtaggtaATAACCCTTTTCCCTCTTTCAGAGTAATTAGCTTCTTTTGTTTTAACAGTAAAAGTGCGCGACCAAGGTTGGTCGGGTCGTTGGGGATCGCCACTGTCGCGCCTTCTTTTATTTGCGCCACGGTTTTGAttttttt                                                                            <  2:380596/143‑1 (MQ=255)

                                                                     gtaggtaATAACCCTTTTCCCTCTTTCAGAGTAATTAGCTTCTCTTTTTGCAACAGTAAAAGTGCGCGACCAAGGTTGGTCGGGTCGTTGGGGATCGCCACTGTCGCGCCTTCTTTTATTTGCGCCACGGTTTTGAgtttttt                                                                            >  1:111387/1‑143 (MQ=255)

                                                                     gtaggtaATAACCCTTGTCCCTCTTTCAGAGTAATTAGCTTCTCTTTTTGCAACAGTAAAAGTGCGCGACCAAGGTTGGTCGGGTCGTTGGGGATCGCCACTGTCGCGCCTTCTTTTATTTGCGCCACGGTTTTGAttttttt                                                                            <  1:757860/143‑1 (MQ=255)

                                                                      taggtaACGACCCTTTTCCCTCTTTCAGAGTAATTAGCTTCTCTTTTTGCAACAGTAAAAGTGCGCGACCAAGGTTGGTCGGGTCGTTGGGGATCGCCACTGTCGCGCCTTCTTTTATTTGCGCCACGGTTTTGATTTTTTTg                                                                           <  2:304124/143‑1 (MQ=255)

                                                                       aggtaATAACCCTTTTCCCTCTTTCAGAGTAATTAGCTTCTCTTTTTGCAACAGTAAAAGTGCGCGACCAAGGTTGGTCGGGTCGTTGGGGATTGCCACTGTCGCGCCTTCTTTTATTTGCGCCACGGTTTTGATTTTTTTgg                                                                          <  1:86461/143‑1 (MQ=255)

                                                                       aggtaATAACCCTTTTCCCTCTTTCAGAGTAATTAGCTTCTCTTTTTGCAACAGTAAAAGTGCGCGACCAAGGTTGGTCGGGTCGTTGGGGATCGCCACTGTCGCGCCTTCTTTTATTTGCGCCACGGTTTTGATTTTTTTgg                                                                          >  2:762138/1‑143 (MQ=255)

                                                                           aataaCCCTTTGCCCTCTTTCCGAGTAATTAGCTTCTCTTTGTGCAACAGTAAAAGTGCGCGACCAAGGTTTGTCGGGTCGTTGGGTATCGCCACTGTCGCGCCTTCTTTTATTTGCGCCACGGTTTTGATTTTTTTGGAATa                                                                      <  2:124371/143‑1 (MQ=255)

                                                                            ataaCCCCTTTCCCTCTTTCAGAGTAATTAGCTTCTCGTTTTGCACCAGTACAAGTGCGCGACCACGGTTTGTCGGGTCGTTGGGGATCGCCACTGTCGCGCCTTCTTCTATTTGCGCCACGGTTTTGATTTTTTTGGAATaa                                                                     <  2:1105863/143‑1 (MQ=255)

                                                                             taaCCCTTTTCCGTCTTTCAGAGTAATTAGCTTCTCGTGTTGCACCAGTAAAAGTGCGCGACCAAGGTGGGTCTGGTCGTTGGGGATCGCCACTGTCGCGCCTTCTTTTATTTGCGCCACGGTTTTGATTTTTTTGGAATAAc                                                                    <  2:844247/143‑1 (MQ=255)

                                                                               attcTTTTCCCTCTTTCGTAGTAATTAGCTTCTTTTTTTGCAACAGTAAAATTGCGCGACCTAGGTTGTTCGGGTTGTTGTGGATCGCTATTGTCGCGCCTTCTTTTATTTGCGCTACGGTTTTGATTTTTTTGGAATAAccc                                                                  <  2:590129/140‑1 (MQ=255)

                                                                                cccTTTTCCCTCTTTCAGAGTAATTAGCTTCTCTTTTTGCAACAGTAAAAGTGCGCGACCAAGGTTGGTCGGGTCGTTGGGGATCGCCACTGTCGCGCCTTCTTTTATTTGCGCCACGGTTTTGATTTTTTTGGAATCACCCg                                                                 >  1:524841/1‑143 (MQ=255)

                                                                                 ccTTTTCCCTCTTTCAGAGTAATTAGCTTCTCTTTTTGCAACAGTAAAAGTGCGCGACCAAGGTTGGTCGGGTCGTTGGGGATCGCCACTGTCGCGCCTTCTTTTATTTGCGCCACGGTTTTGATTTTTTTGGAATAACCCGc                                                                <  1:1163504/143‑1 (MQ=255)

                                                                                 ccTTCTCCCTCTTTCAGAGGAATTAGCGTCTCTTTTTGCAACAGTAAAAGTGCGCGACCAAGGTTGGTCGGGTCGTTGGGGATCGCCACTGTCGCGCCTTCTTTTATTTGCGCCACGGTTTTGATTTTTTTGGAATAACCCGc                                                                <  1:381561/143‑1 (MQ=255)

                                                                                     ttCCCTCTTTCAGAGTAATTAGCTGCTCTTTTTGCAACAGTAAAAGTGCGCGACCAAGGTTGGTCGGGTCGTTGGGGATCGCCACTGTCGCGCCTTCTTTTATTTGCGCCACGGTTTTGATTTTTTTGGAATAACCCGCCAT‑a                                                           <  1:887515/143‑1 (MQ=255)

                                                                                          tctTTCAGAGTAATTAGCTTCTCTTTTTGCAGCAGTAAAAGTGCGCGACCAAGGTTGGTCGGGTCGTTGGGGATCGCCACTGTCGCGCCTTCTTTTATTTGCGCCACGGTTTTGATTTTTTTGGAATAACCCGCCAT‑AGGGaa                                                      <  2:1062032/143‑1 (MQ=255)

                                                                                          tctTTCAGAGTAATTAGCTTCTCTTTTTGCAACAGTAAAAGTGCGCGACCAAGGTTGGTCGGGTCGTTGGGGATCGCCACTGTCGCGCCTTCTTTTATTTGCGCCACGGTTTTGATTTTTTTGGAATAACCCGCCAT‑AGGGaa                                                      <  2:524336/143‑1 (MQ=255)

                                                                                          tctTTCAGAGTAATTAGCTTCTCTTTTTGCAACAGTAAAAGTGCGCGACCAAGGTTGGTCGGGTCGTTGGGGATCGCCACTGTCGCGCCTTCTTTTATTTGCGCCACGGTTTTGATTTTTTTGGAATAACCCGCCAT‑AGGGaa                                                      <  1:677391/143‑1 (MQ=255)

                                                                                          tctTTCAGAGTAATTAGCTTCTCTTTTTGCAACAGTAAAAGTGCGCGACCAAGGTTGGTCGGGTCGTTGGGGATCGCCACTGTCGCGCCTTCTTTTATTTGCGCCACGGTTTTGATTTTTTTGGAATAACCCGCCAT‑AGGGaa                                                      >  1:1105923/1‑143 (MQ=255)

                                                                                          tctTTCAGAGTAATTAGCTTCTCTTTTTGCAACAGTAAAAGTGCGCGACCAAGGTTGGTCGGGTCGTTGGCGATCGCCACTGTCGCGCCTTCTTGTATTTGCGCCACGGTTTTGATTTTTTTGGAATAACCCGCCAT‑AGGGaa                                                      >  2:840852/1‑143 (MQ=255)

                                                                                            tttCAGAGTAATTAGCTTCTCTTTTTGCAACAGTAAAAGTGCGCGACCAAGGTTGGTCGGGTCGTTGGGGATCGCCACTGTCGCGCCTTCTTTTATTTGCGCCACGGTCGTGATTTTTTTAGACTAACCCGCCAT‑AGAGACGa                                                    >  2:681675/1‑143 (MQ=255)

                                                                                             ttCAGAGTAATTAGCTTCTCTTTTTGCAACAGTAAAAGTGCGCGCCCAAGGTTGGTCGGGTCGTTGGGGATCGCCACTGTCGCGCCTTCTTTTATTTGCGCCACGGTTTTGGCTTTTTTGGCATAACCCGCCAT‑AGGGATGAc                                                   >  2:321304/1‑143 (MQ=255)

                                                                                             ttCAGAGTAATTAGCTTCTCTTTTTGCAACAGTAAAAGTGCGCGACCAAGGTTGGTCGGGTCGTTGGGGATCGCCACTGTCGCGCCTTCTTTTATTTGCGCCACGGTTTTGATATTTTTGGACTAACCCGCCAT‑Agtgcggag                                                   >  2:863447/1‑136 (MQ=255)

                                                                                                 gagTAATTAGCTTCTCTTTTTGCAACAGTAAAAGTGCGCGACCAAGGTTGGTCGGGTCGTTGGGGATCGCCACTGTCGCGCCTTCTTTTATTTGCGCCACGGTTTTGATTTTTTTGGAATAACCCGCCAT‑AGGGAAGACAAAc                                               <  1:141352/143‑1 (MQ=255)

                                                                                                  agTAATTAGCTTCTCTTTTTGCAACAGTAAAAGTGCGCGACCAAGGTTGGTCGGGTCGTTGGGGATCGCCACTGTCGCGCCTTCTTTTATTTGCGCCACGGTTTTGATTGTTTTGGAATAACCCGCCAT‑AGGGAAGACAAACg                                              >  1:430094/1‑143 (MQ=255)

                                                                                                      aTTAGTTTCTCTTGTTGCAACAGGAAAAGCGCGCGGCCAAGGTTGGTCGGGTCGTTGTGGATCGCCACTGTCGCGCCTTCTTTTATTTGCGCCACGGTTTTGATTTTTTTGGAATAACCCGCCAT‑AGGGAACACAAACGTAtt                                          <  2:331432/143‑1 (MQ=255)

                                                                                                         aGCTTCTCTTTTTGCAACAGTAAAAGTGCGCGACCAAGGTTGGTCGGGTCGTTGGGGATCGCCACTGTCGCGCCTTCTTTTATTTGCGCCACGGTTTTGATTTTTTTGGAATAACCCGCCAT‑AGGGAAGACAAACGTATTTcc                                       <  2:244229/143‑1 (MQ=255)

                                                                                                           cTTCTCTTTTTGCAACAGTAAAAGTGCGCGACCAAGGTTGGTCGGGTCGTTGGGGATCGCCACTGTCGCGCCTTCTTTTATTTGCGCCACGGTTTTGATTTTTTTGGAATAACCCGCCAT‑AGGGAATGCAAACGTATTTctct                                     >  2:1184838/1‑140 (MQ=255)

                                                                                                           cTTCTCTTTTTGCAACAGTAAAAGTGCGCGACCAAGGTTGGTCGGGTCGTTGGGGATCGCCACTGTCGCGCCTTCTTTTATTTGCGCCACGGTTTTGATTTTCTTGGACTAACCCGCCAT‑AGGGAAGACAAACGTATTTTCCa                                     >  2:583377/1‑143 (MQ=255)

                                                                                                            gtctctcTTTCCAAGATTACAAGTGCGCAACCAAGGTTGGTCGGGTCGTTGGGGATCGCCACTGTCGCGCCTTCTTTTATTTGCGCCACGGTTTTGATTTTTTTGGAATAACCCGCCAT‑AGGGAAGACAAACGTATTTCCCAc                                    <  1:351922/142‑1 (MQ=255)

                                                                                                              ctctTTTTGCAACAGTAAAAGTGCGCGACCAAGGTTGGTCGGGTCGTTGGGGCTCGCCACCGCCGCCACATGTGTTATTTGCGCCACGGTTTTGATTTTTTTGGGATAACCCGCCAT‑CGGGGAGACAAACGTAGTTCCCAccc                                  >  1:479165/1‑142 (MQ=255)

                                                                                                              ctctTTTTGCAACAGTAAAAGTGCGCGACCAAGGTTGGTCGGGTCGTTGGGGATCGCCACTGTCGCGCCTTCTTTTATTTGCGCCACGGTTTTGATTTTTTTGGAATAACCCGCCAT‑AGGGAAGACAAACGTATTTCCCACCg                                  >  1:1142579/1‑143 (MQ=255)

                                                                                                                ctTGTTGCAACAGTAAAAGTGCGCGACCCAGGTTGGTCGGGTCGTTGGGGATCGCCACTGTCGCGCCTTCTTTTATTTGCGCCACGGTTTTGATTTTTTTGGAATAACCCGCCAT‑AGGGAAGACAAACGTATTTCCCACCGCt                                <  2:684735/143‑1 (MQ=255)

                                                                                                                   tttGCAACAGTAAAAGTGCGCGACCAAGGTTGGTCGGGTCGTTGGGGATCGCCACTGTCGCGCCTTCTTTTATTTGCGCCACGGTTGTGATTTTTTTGGACTAACCCGCCAT‑AGGGAAGACAAACGGATTTCCAACCGCTAcc                             >  2:72416/1‑143 (MQ=255)

                                                                                                                    ttGCAACGGTAAAAGTGCGCGACCAAGGTTGGTCGGGTCGTTGGGGATCGCCACTGTCGCGCCTTCTTTTATTTGCGCCACGGTTTTGATTTTTTTGGAATAACCCGCCAT‑AGGGAAGACAAACGTATTTCCCACCGCTACCa                            <  2:164816/143‑1 (MQ=255)

                                                                                                                        aaCAGTAAAAGTGCGCGACCAAGGTTGGTCGGGTCGTTGGGGATCGCCACTGTCGCGCCTTCTTTTATTTGCGCCACGGTTTTGATTTTTTTGGAATAACCCGCCAT‑AGGGGAGGCAACCGTCGTGCCGACAGCTAcgcggtg                        >  2:861955/1‑137 (MQ=255)

                                                                                                                         aCAGTAAAAGTGCGCGACCAAGGTTGGTCGGGTCGTTGGGGATCGCCACTGTCGCGCCTTCTTTTATTTGCGCCACGGTTTTGCTTTTTTTGGAATAACCTCTGCT‑CAGCAAGACAGACGTATTTCCTACCGCTACCAGTTta                       >  2:562055/1‑143 (MQ=255)

                                                                                                                             tAAAAGTGCGCGACCAAGGTTGGTCGGGTCGTTGGGGATCGCCACTGTCGCGCCTTCGTTTATTTGCGCCCCGGTTTTGATTTTTTTGGAATAACCCGCCAT‑CGGGAAGACAAACGTATTTCCCACCGCTACCAGTTTATAAc                   >  1:630565/1‑143 (MQ=255)

                                                                                                                                tggTGCGCGACCGAGGTTGGGCGGGTCGTTGGGGATCGCCACTGTCGCGCCTTCTTTTATTTGCGCCACGGTTTTGATTTTTTTGGAATAACCCGCCAT‑AGGGAAGACAAACGTATTTCCCACCGCTACCAGTTTATAACCAt                <  2:378549/141‑1 (MQ=255)

                                                                                                                                aaGTGCGCGAGCAAGCTTGGTCGGGTCTTTTGGGATCTCCTCTGTCCCGCCTTCTTTTATTTGCGCCACGGTTTTGATTTTTTTGGAATAACCCGCCAT‑AGGGAAGACAAACGTATTTCCCACCGCTACCAGTTTATAACCAt                <  2:1274035/143‑1 (MQ=255)

                                                                                                                                aaGTGCGCGACCAAGGTTGGTCGGGTCGTTGGGGATCGCCACTGTCGCGCCTTCTTTTATTTGCGCCACGGTTTTGATTTTTTTGGAATAACCCGCCAT‑AGGGAAGACAAACGTATTTCCCACCGCTACCAGTTTATAACCAt                <  1:262188/143‑1 (MQ=255)

                                                                                                                                 aGTGCGCGACCAAGGTTGGTCGGGTCGTTGGGGATCGCCACTGTCGCGCCTTCTTTTATTTGCGCCACGGTTTTGATTTTTTTGGAATAACCCGCCAT‑AGGGAAGACGAACGTCTTTCCCACCGCTACCAGTTTATAAGCATg               >  1:1006567/1‑143 (MQ=255)

                                                                                                                                     cccgACCAACCTTGGTCGGGTCGTTGGGGATCGCCACTGTCGCGCCTTCTTTTATTTGCGCCACGGTTTTGATTTTTTTGGAATAACCCGCTAT‑AGGGAAGACAAACGTATTTCCCACCGCTACCCGTTTATAACCAttcccc           >  2:911357/3‑138 (MQ=255)

                                                                                                                                     cccgACCAACCTTGGTCGGGTCGTTGGGGATCGCCACTGTCGCGCCTTCTTTTATTTGCGCCACGGTTTTGATTTTTTTGGAATAACCCGCCAT‑AGGGCAGCAAACCGCTTTTCCCACCGGTACCCGTCTATCACCATgcggc           >  1:232695/3‑141 (MQ=255)

                                                                                                                                     cccgACCAACCTTGGTCGGGTCGTTGGGGATCGCCACTGTCGCGCCTTCTTTTATTTGCGCCACGGTTTTGA‑TTTTTTGGAATAACCCGCCATAAGTGAAG‑CAAACGTAGTTCCCACCGCCACCAGATTATAACCATGCGACt          >  2:710571/3‑143 (MQ=255)

                                                                                                                                       cgACCAGGGGTGGTTGGGTCGTTGGGGATCGCCACTGTCGCGCCTTCTGTTATTTGCGCCAGGGTTTTGATTTTTTTGGAATAACCCGCCCT‑AGGGAAGACAAACGTATTTCTCACCGCTACCAGTTTATAACCATGCGCCTg         <  2:161755/143‑1 (MQ=255)

                                                                                                                                        gACCAAGGTTGGTCGGGTCGTTGGGGATCGCCACTGTCGCGCCTTCTTTTATTTGCGCCACGGTTTTGATTTTTTTGGAATAACCCGCCAT‑AGGGAAGACAAACGTATTTCCCACCGCTACCAGTTTATAACCATGCGCCTGa        >  1:621312/1‑143 (MQ=255)

                                                                                                                                            aaGGGTGGTCGGGTCGTTGGGGATCGCCACTGTCGCGCCTTCTTTTATTTGCGCCACGGTTTTGATTTTTTTGGAATAACCCGCCAT‑AGGGAAGACAAACGTATTTCCCACCGCTACCAGTTTATAACCATGCGCCTGATTAt    <  1:510224/143‑1 (MQ=255)

                                                                                                                                             aGGTTGGTCGGGTCGTTGGGGCTCGCCACTGTCGCGCCTTCTTTTATTTGCGCCACGGTTTTGATTTTTTTGGAATAACCCGCCAT‑AGGGAAGACAAACGTATTTCCCACCGCTACCAGTTTATAACCATGCGCCTGATTATc   <  1:1049522/143‑1 (MQ=255)

                                                                                                                                             aGGTGGGTCGGGTCGTTGGGGATCGCCACTGTCGCGCCTTCTTTTATCTCCGCCACTTTTTTGATTTTTTTGGAATACCCCGCCAT‑AGGGAAGACAAACGTATTTCCCACCGCTACCAGTTTATAACCATGCGCCTGATTATc   <  1:412293/143‑1 (MQ=255)

CGCGGGAGCTGCGCCCCCTCGAGTTCCATAATCTGCAAATGACGAGGATTATCGGTTATATCCAGCGCGGTAGGTAATAACCCTTTTCCCTCTTTCAGAGTAATTAGCTTCTCTTTTTGCAACAGTAAAAGTGCGCGACCAAGGTTGGTCGGGTCGTTGGGGATCGCCACTGTCGCGCCTTCTTTTATTTGCGCCACGGTTTTGATTTTTTTGGAATAACCCGCCAT‑AGGGAAGACAAACGTATTTCCCACCGCTACCAGTTTATAACCATGCGCCTGATTATCT  >  NC_000913/3839425‑3839709

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 8 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`