BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	3,839,568	G→C	5.8%	N142K (AAC→AAG)	nlpA ←	lipoprotein‑28

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,839,568	0	G	C	5.8%	322.9 / 10.6	116	N142K (AAC→AAG)	nlpA	lipoprotein‑28
Reads supporting (aligned to +/- strand): ref base G (55/54); new base C (4/3); total (59/57)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.88e-01

Only 100 of 119 total aligned reads displayed.

GCGGGAGCTGCGCCCCCTCGAGTTCCATAATCTGCAAATGACGAGGATTATCGGTTATATCCAGCGCGGTAGGTAATAACCCTTTTCCCTCTTTCAGAGTAATTAGCTTCTCTTTTTGCAACAGTAAAAGTGCGCGACCAAGGTTGGTCGGGTCGTTGGGGATCGCCACTGTCGCGCCTTCTTTTATTTGCGCCACGGTTTTGATTTTTTTGGAATAACCCGCCAT‑AGGGAAGACAAACGTATTTCCCACCGCTACCAGTTTATAACCATGCGCCTGATTATCT  >  NC_000913/3839426‑3839709

gcgcGAGCTGCGCCCCCTCGAGTTCCATAATCTGCAAATGACGAGGATTATCGGTTATATCCAGCGCGGTAGGTAATAACCCTTTTCCCTCTTTCAGAGTAATTAGCTTCTCTTTTTGCAACAGTAAAAGTGCGCGACCAAgg                                                                                                                                                <  1:35130/143‑1 (MQ=255)

gcgGGAGCTGCGCCCCCTCGAGTTCCATAATCTGCAAATGACGAGGATTATCGGTTATATCCAGCGCGGTAGGTAATAACCCTTTTCCCTCTTTCAGAGTAATTAGCTTCTCTTTTTGCAACAGTAAACGTGCGCGACCAAgg                                                                                                                                                >  1:378549/1‑143 (MQ=255)

 cgGGAGCTGCGCCCCCTCGAGTTCCATAATCTGCAAATGACGAGGATTATCGGTTATATCCAGCGCGGTAGGTAATAACCCTTTTCCCTCTTTCAGAGTAATTAGCTTCTCTTTTTGCAACAGTAAAAGTGCGCGACCAAGGt                                                                                                                                               <  1:1073189/143‑1 (MQ=255)

 cgGGAGCTGCGCCCCCTCGAGTTCCATAATCCGGTAAGGACGGGGATTATCGGTTATATCCAGCGCGGTAGGTAATAACCCTTTTCCCTCTTTCAGAGTAATTAGCTTCTCTTTTTGCAACAGTAAAAGTGCGCGACCAAGGt                                                                                                                                               <  1:992185/143‑1 (MQ=255)

       cTGCGCCCCCTCGAGTTCCATAATCTGCAAATGACGAGGATTATCGGTTATATCCAGCGCGGTAGGTAATAACCCTTTTCCCTCTTTCAGAGTAATTAGCTTCTCTTTTTGCAACAGTAAAAGTGCGCGACCAAGGTTggtcg                                                                                                                                         <  2:1235781/143‑1 (MQ=255)

         gcgcCCCCTCGAGTTCCATAATCTGCAAATGACGAGGATTATCGGTTATATCCAGCGCGGTAGGTAATAACCCTTTTCCCTCTTTCAGAGTAATTAGCTTCTCTTTTTGCAACAGTAAAAGTGCGCGACCAAGGTTggccggg                                                                                                                                       >  1:195316/1‑143 (MQ=255)

             ccccTCGAGTTCCATAATCTGCAAATGACGAGGATTATCGGTTATATCCAGCGCGGTAGGTAATAACCCTTTTCCCTCTTTCAGAGTAATTAGCTTCTCTTTTTGCAACAGTAAAAGTGCGCGACCAACCTTggtcgcgcact                                                                                                                                   <  1:263630/143‑7 (MQ=255)

             ccccTCGAGTTCCATAATCTGCAAATGACGAGGATTATCGGCTATATCCAGCGCGGTAGGTAATAACCCTTTTCCCTCTTTCAGAGTAATTAGCTTCTCTTTTTGCAACAGTAAAAGTGCGCGACCAAGGTTGGTCGAGTCGt                                                                                                                                   >  2:359031/1‑143 (MQ=255)

               ccTCGAGTTCCATAATCTGCAAATGACGAGGATTATCGGTTATATCCAGCGCGGTAGGTAATACCCCTTTTCCCCCTTCCAGAGCAAATCGCTTCTCTTTCTGCAACAGTCACAGTGCGCGACCACGGTTAGTCGAGTCGTTg                                                                                                                                 >  2:1186856/1‑143 (MQ=255)

               ccTCGAGTTCCATAATCTGCAAATGACGAGGATTATCGGTTATATCCAGCGCGGTAGGTAATAACCCTTTTCCCTCTTTCAGAGTAATTAGCTTCTCTTTTTGCAACAGTAAAAGTGCGCGACCAATGTTGGTCGGGTCGTTg                                                                                                                                 <  2:276092/143‑1 (MQ=255)

                cTCGAGTTCCATAATCTGCAAATGACGAGGATTATCGGTTATATCCAGCGCGGTAGGTAATAACCCTTTTCGCTCTTTCAGAGTAATTAGCTTATCTTTTCGCAACAGTAAAAGTGCGCGACCAAGGTTGGCAGGGTCGTggg                                                                                                                                >  2:141352/1‑143 (MQ=255)

                cTCGAGTTCCATAATCTGCAAATGACGAGGATTATCGGTTATATCCAGCGCGGTAGGTAATAACCCTTTTCCCTCTTTCAGAGTAATTAGCTTCTCTTTTTGCAACAGTAAAAGTGCGCGACCAAGGTTGGTCGGGTCGTTgg                                                                                                                                >  2:1258321/1‑143 (MQ=255)

                 tCGAGTTCCATAATCTGCAAATGACGAGGATTATCGGTTATATACAGCGCGGTAGGTAATAACCCTTGTCCCTCTTTCAGAGCAATTAGCTTCTCTTTTTGCAACAGTAACAGTGCGCGACCAAGGTGGGTCGGGTCGTTggg                                                                                                                               >  2:699773/1‑143 (MQ=255)

                 tCGAGTGCCGTAGTGTGTAAATGACGAGGATTATCTGTCATATCCAGCGCGGTAGGTAATAACCCTTTTCCCTATTTCAGAGTAATTAGCTTCTCTTTTTGCAACAGTAAAAGTGCGCGACCAAGGTTGGTCGGGTCGTTggg                                                                                                                               <  2:414891/143‑1 (MQ=255)

                  cGAGTTCCATAATCTGCAAATGACGAGGATTATCGGTTATATCCAGCGCGCTAGGTAATAACCCTTTTCCCTCTTTCAGAGTAATTAGCTTCTCTTTTTGCAACAGTAAAAGTGCGCGACCAAGGTTGGTCGGGTCGTTgggg                                                                                                                              >  2:53664/1‑143 (MQ=255)

                       tCCATAATCTGCAAATGACGAGGATTATCGGTTATATCCAGCGCGGTAGGTAATAACCCTTTTCCCTCTTTCAGAGTAATTAGCTTCTCTTTTTGCAACAGTAAAAGTGCGCGACCAAGGTTGGTCGGGTCGTTGGGGATCGc                                                                                                                         >  2:188984/1‑143 (MQ=255)

                               acggagaTGACGAGGATTATCGGTTATATCCCGCGCGGTAGGTAATAGCCCTTTTCTCTCTTTCAGAGTAATTAGCTTCTCTTTTTGCAACAGTAAAAGTGCGCGACCAAGGTTGGTCGGGTCGTTGGGGATCGCCACTGTcg                                                                                                                 <  2:236317/137‑1 (MQ=255)

                                 gCAAATGACTAGGATTATCGGTTATATCCAGCGCGGTAGGTAATAACCCTTTTCCCTCTTTCAGAGTAATTAGCTTCTCTTTTTGCAACAGTAAAAGTGCGCGACCAAGGTTGGTCGGGTCGTTGGGGATCGCCACTGTcgcg                                                                                                               <  1:917493/143‑1 (MQ=255)

                                 ccAAATGACGAGCATTATCGGTTATATCCAGCGCGGTAGGTAATATCCCTTTTCCCTCTTGCAGAGTAATTAGCTTCTCTTTTTGCAACAGTAAAAGTGCGCGACCAAGGTTGGTCGGGTCGTTGGGGATCGCCACTGTcgcg                                                                                                               <  2:810749/142‑1 (MQ=255)

                                  caactcacgacgATTACCGGTTATATCCAGCGCGGTAGCTAATAACCCTGTTCCCTCTTTCAGAGTAATTAGCTTCTCTTTTTGCAACAGTAAAAGTGCGCGACCAAGGTTGGTCGGGTCGTTGGGGATCGCCACTGTCgcgc                                                                                                              <  2:896701/137‑1 (MQ=255)

                                    aaTGACGAGGATTATCGGTTATATCCAGCGCGGTAGGTAATAACCCTTTTCCCTCTTTCAGAGTAATTAGCTTCTCTTTTTGCAACAGTAAAAGTGCGCGACCAAGGTTCGTCGGGTCGTTGGGGATCGCCCCTGTCgcgcgt                                                                                                            >  2:1009536/1‑141 (MQ=255)

                                     aTGACGAGGATTATCGGTTATATCCAGCGCGGTAGGTAATAACCCTTTTCCCTCTTTCAGAGTAATTAGCTTCTCTTTTTGCAACAGTAAAAGTGCGCGACCAAGGTTGGTCGGGTCGTTGGGGATCGCCACTGTCGCGCctt                                                                                                           >  1:547510/1‑143 (MQ=255)

                                     aTGACGAGGATTATCGGTTATATCCAGCGCGGTAGGTAATAACCCTTTTCCCTCTTTCAGAGAAATTAGCTTCTCTTTTTGCAACAGTAAAAGTGCGCGACCAAGGTTGGTCGGGTCGTTGGGGATCGCCACTGTCGCGCctt                                                                                                           <  2:704940/143‑1 (MQ=255)

                                         cGATGATTATCGGTTATATCCAGCGCGGTAGGTAATAACCCTTTTCCCTCTTTCAGAGTAATTAGCTTCTCTTTTTGCAACAGTAAAAGTGCGCGACCAAGGTTGGTCGGGTCGTTGGGGATCGCCACTGTCGCGCCTTCttt                                                                                                       <  2:115398/143‑1 (MQ=255)

                                          gAGGATTATCGGTTATATCCAGCGCGGTAGGTAATAACCCTTTTCCCTCTTTCAGAGTAATTAGCTTCTCTTTTTGCAACAGTAAAAGTGCGCGACCAAGGTTGGTCGGGTCGGTGGGGATCGCCGCTGTCGCGCCGTCtttt                                                                                                      >  1:369561/1‑143 (MQ=255)

                                             gATTATCGGTTATATCCAGCGCGGTAGGTAATAACCCTTTTCCCTCTTTCAGAGTAATTAGCTTCTCTTTTTGCAACAGTAAAAGTGCGCGACCAAGGTTGGTCGGGTCGTTGGGGATCGCCACTGTCGCGCCTTCTTTTAtt                                                                                                   <  1:1234079/143‑1 (MQ=255)

                                             gATTATCGGTTATATCCAGCGCGGTAGGTAATAACCCTTTTCCCTCTTTCAGAGTAATTAGCTTCTCTTTTTGCAACAGTAAAAGTGCGCGACCAAGGTTGGTCGGGTCGTTGGGGATCGCCACTGTCGCGCCTTCTTTTAtt                                                                                                   <  1:1012861/143‑1 (MQ=255)

                                               ttttCGGTTATATCCAGCGCGGTAGGTAATAACCCTTTTCCCTCTTTCAGAGTAATTAGCTTCTCTTTTTGCAACAGTAAAAGTGCGCGACCAAGGTTGGTCGGGTCGTTGGGGATCGCCACTGTCGCGCCTTCTTTTATTTg                                                                                                 <  2:297057/140‑1 (MQ=255)

                                                tATCGGTTATATCCAGCGCGGTAGGTAATAACCCTTTTCCCTCTTTCAGAGTAATTAGCTTCTCTTTTTGCAACAGTAAAAGTGCGCGACCAAGGTTGGTCGGGTCGTTGGGGATCGCCACTGTCGCGCCTTCTTTTATTTgc                                                                                                <  1:384892/143‑1 (MQ=255)

                                                tATCGGTTATATCCAGCGCGGTAGGTAATAACCCTTTTCCCTCTTTCAGAGTAATTAGCTTCTCTTTTTGCAACAGTAAAAGTGCGCGACCAAGGTTGGTCGGGTCGTTGGGGATCGCCACTGTCGCGCCTTCTTTTATTTgc                                                                                                >  1:706713/1‑143 (MQ=255)

                                                tATCGGTTATATCCAGCGCGGTAGGTAATAACCCTTTTCCCTCTTTCAGAGTAATTAGCTTCTCTTTTTGCAACAGTAAAAGTGCGCGACCAAGGTTGGTCGGGTCGTTGGGGATCGCCACTGTAGCGCCTTCTTTTATTTgc                                                                                                >  2:499346/1‑143 (MQ=255)

                                                tATCGGCTATATCCAGCGCGGTAGGTAATAACCCTTTTCCATCTTTCAGAGTAATTAGCTTCTCTTTTTGCAACAGTAAAAGTGCGCGACCAAGGTTGGTCGGGTCGTTGGGGATCGCCCCTGTCGCGGCTTCTTGTAtttcc                                                                                                >  1:470374/1‑141 (MQ=255)

                                                  tCGGTTATATCCAGCGCGGTAGGTAATAACCCTTTTCCCTCTTTCAGAGTAATTAGCTTCTCTTTTTGCAACAGTAAAAGTGCGCGACCAAGGTTGGTCGGTGCGTTGCGGATCGCCACGGTCGCGCCTTCTTTTGTTTgtgc                                                                                              >  2:922722/1‑143 (MQ=255)

                                                  tCGGTTATATCCAGCGCGGTAGGTAATAACCCTTTTCCCTCTTTCAGAGTAATTAGCTTCTCTTTTTGCAACAGTAAAAGTGCGCGACCAAGGTTGGTCGGGTCGTTGGGGCTCGCCACTGTCGCGCCTTCTTTTGTTTgcgc                                                                                              >  1:306302/1‑143 (MQ=255)

                                                  tCGGTTATATCCAGCGCGGTAGGTAATAACCCTTTTCCCTCTTTCAGAGTAATTAGCTTCTCTTTTTGCAACAGTAAAAGTCCGCGCCCTAGGTTGGTCGGGTCGTTGGGGATCGCCACTGTCGCCCCTTCTTTTCTTTgcgc                                                                                              >  1:1105083/1‑143 (MQ=255)

                                                     gTTATATCCAGCGCGGTAGGTAATAACCCTTTTCCCTCTTTCAGAGTAATTAGCTTCTCTTTTTGCAACAGTAAAAGTGCGCGACCAAGGTTGGTCGGGTCGTTGGGGATCGCCACTGTCGCGCCTTCTTTTATTTGCGCCAc                                                                                           >  1:678042/1‑143 (MQ=255)

                                                      ttATATCCAGCGCGGTAGGTAATAACCCTTTTCCCTCTTTCAGAGTAATTAGCTTCTCTTTTTGCAACAGTAAAAGTGCGCGACCAAGGTTGGTCGGGTCGTTGGGGATCGCCACTGTCGCGCCTTCTTTTATTTGCGCCACg                                                                                          >  1:1256621/1‑143 (MQ=255)

                                                       tataTCCAGCGCGGTAGGTAATAACCCTTTTCCCTCTTTCAGAGTAATTAGCTTCTCTTTTTGCAACAGTAAAAGTGCGCGACCAAGGTTGGTCGGGTCGTTGGGGATCGCCACTGTCGCGCCTTCTTTTATTTGCGCCACgg                                                                                         >  2:662206/1‑143 (MQ=255)

                                                         ttcccAGCGCGGTAGGTAATGACCCTTTTTCCTCTTTCAGAGTAATTAGCTTCTCTTTTTGCAACAGTAAAAGTGCGCGACCAAGGTTGGTCGGGTCGTTGGGGATCGCCACTGTCGCGCCTTCTTTTATTTGCGCCACGGtt                                                                                       <  2:120755/140‑1 (MQ=255)

                                                          atCCAGCGCGGTAGGTAATAACCCTTTTCCCTCTTTCAGAGTAATTAGCTTCTCTTTTTGCAACACTAAAAGTGCGCGACCAAGGTTGGTCGGGTCGTTGGGGATCGCCACTGTCGCGCCTCCTTTTATTTGCCCCCCGGctt                                                                                      >  1:1234635/1‑143 (MQ=255)

                                                          atCCAGCGCGGTAGGTAATAACCCTTTTCCCTCTGTCAGCGTAAGTAGCTTCTCTTTTTGCAACAGTAAAAGTGCGCGACCAAGGTTGGTCGGGTCGTTGGGGATCGCCACTGTCGCGCCTTCTTTTATTTGCGCCACGGttt                                                                                      >  1:244229/1‑143 (MQ=255)

                                                            ctatcgcgGTAGGTAATACTCCCTTTCCCTCTTTCAGAGTAATTAGCTTCTCTTTTTGCAACAGTAACAGTTCGCGACTAAGGTTTGTCGGGTCGTTCGGGATCTCCACTGTCGCTCCTTCTTTTATTTGCGCCACGGTTTTg                                                                                    <  2:104772/139‑1 (MQ=255)

                                                             taGCGCGGTAGGTAATAACCCTTTTCCCTCTTTCAGAGTAATTAGCTTCTCTTTTTGCAACAGTAAAAGTGCGCGACCAAGGTTGGTCGGGTCGTTGGGGATCGCCACTGTCGCGCCTTCTTTTATTTGCGCCACGGTTTTgg                                                                                   >  1:760064/2‑142 (MQ=255)

                                                              aGCGCGGTAGGTAATAACCCTTTTCCCTCTTTCAGAGTAATTAGCTTCTCTTTTTGCAACAGTAAAAGTGCGCGACCAAGGTTGGTCGGGTCGTTGGGGATCGCCACTGTCGCGCCTTCTTTTCTTTGCGCCCCGGtttcgct                                                                                  >  1:112942/1‑139 (MQ=255)

                                                              aGCGCGGTAGGTAATAACCCTTTTCCCTCTTTCAGAGTAATTAGCTTCTCTTTTTGCAACAGTAAAAGTGCGCGACCAAGGTTGGTCGGGTCGTTGGGGATCGCCACTGTCGCGCCTTCTTTTATTTGCGCCACGGTTTTgtt                                                                                  >  1:784282/1‑141 (MQ=255)

                                                                  cgGTAGGTAATAACCCTTTTCCGTCTTTCCGCGTAATTAGCTTCTCTTTTTGCAACAGTAAAAGTGCGCGACCAAGGTTGGTCGGGTCGTTGGGGATCGCCACTGTCGCGCCTTCTTTTATTTGCGCCACGGTTTTGAttttt                                                                              <  2:1062041/143‑1 (MQ=255)

                                                                   ggtggttaataaCCCTTTTCCCTCTTTCAGAGTAATTAGCTTCTCTTTTTGCAACAGTAAAAGTGCGCGACCAAGGTTGGTCGGGTCGTTGGGGATCGCCACTGTCGCGCCTTCTTTTATTTGCGCCACGGTTTTGAtttttt                                                                             <  1:1086601/137‑1 (MQ=255)

                                                                    ttcggtaATATCCCTTGTCCCTCTTTCAGAGTAATTAGCGTCTCTTTTTGCAACAGTACAAGTGCGCGACCAAGGTTGGTCGGGTCGTTGGGGATCGCCACTGTCGCGCCTTCTTTTATTTGCGCCACGGTTTTGAttttttt                                                                            <  2:658163/140‑1 (MQ=255)

                                                                    gtaggtaATAACCCTTTTCCCTCTTTCAGAGTAATTAGCTTCTTTTGTTTTAACAGTAAAAGTGCGCGACCAAGGTTGGTCGGGTCGTTGGGGATCGCCACTGTCGCGCCTTCTTTTATTTGCGCCACGGTTTTGAttttttt                                                                            <  2:380596/143‑1 (MQ=255)

                                                                    gtaggtaATAACCCTTTTCCCTCTTTCAGAGTAATTAGCTTCTCTTTTTGCAACAGTAAAAGTGCGCGACCAAGGTTGGTCGGGTCGTTGGGGATCGCCACTGTCGCGCCTTCTTTTATTTGCGCCACGGTTTTGAgtttttt                                                                            >  1:111387/1‑143 (MQ=255)

                                                                    gtaggtaATAACCCTTGTCCCTCTTTCAGAGTAATTAGCTTCTCTTTTTGCAACAGTAAAAGTGCGCGACCAAGGTTGGTCGGGTCGTTGGGGATCGCCACTGTCGCGCCTTCTTTTATTTGCGCCACGGTTTTGAttttttt                                                                            <  1:757860/143‑1 (MQ=255)

                                                                     taggtaACGACCCTTTTCCCTCTTTCAGAGTAATTAGCTTCTCTTTTTGCAACAGTAAAAGTGCGCGACCAAGGTTGGTCGGGTCGTTGGGGATCGCCACTGTCGCGCCTTCTTTTATTTGCGCCACGGTTTTGATTTTTTTg                                                                           <  2:304124/143‑1 (MQ=255)

                                                                      aggtaATAACCCTTTTCCCTCTTTCAGAGTAATTAGCTTCTCTTTTTGCAACAGTAAAAGTGCGCGACCAAGGTTGGTCGGGTCGTTGGGGATTGCCACTGTCGCGCCTTCTTTTATTTGCGCCACGGTTTTGATTTTTTTgg                                                                          <  1:86461/143‑1 (MQ=255)

                                                                      aggtaATAACCCTTTTCCCTCTTTCAGAGTAATTAGCTTCTCTTTTTGCAACAGTAAAAGTGCGCGACCAAGGTTGGTCGGGTCGTTGGGGATCGCCACTGTCGCGCCTTCTTTTATTTGCGCCACGGTTTTGATTTTTTTgg                                                                          >  2:762138/1‑143 (MQ=255)

                                                                       ggtaATAACCCTTTTCCCTCTTTCAGAGTAATTAGCTTCTCTTTTTGCAACAGTAAAAGTGCGCGACCAAGGTTGGTCGGGTCGTTGGGGATCCCCACTGTCGCGCCTTCTTTTATTTGCGCCAGGGTTTTGATTTTTTTgta                                                                         >  2:862245/1‑141 (MQ=255)

                                                                          aataaCCCTTTGCCCTCTTTCCGAGTAATTAGCTTCTCTTTGTGCAACAGTAAAAGTGCGCGACCAAGGTTTGTCGGGTCGTTGGGTATCGCCACTGTCGCGCCTTCTTTTATTTGCGCCACGGTTTTGATTTTTTTGGAATa                                                                      <  2:124371/143‑1 (MQ=255)

                                                                           ataaCCCCTTTCCCTCTTTCAGAGTAATTAGCTTCTCGTTTTGCACCAGTACAAGTGCGCGACCACGGTTTGTCGGGTCGTTGGGGATCGCCACTGTCGCGCCTTCTTCTATTTGCGCCACGGTTTTGATTTTTTTGGAATaa                                                                     <  2:1105863/143‑1 (MQ=255)

                                                                            taaCCCTTTTCCGTCTTTCAGAGTAATTAGCTTCTCGTGTTGCACCAGTAAAAGTGCGCGACCAAGGTGGGTCTGGTCGTTGGGGATCGCCACTGTCGCGCCTTCTTTTATTTGCGCCACGGTTTTGATTTTTTTGGAATAAc                                                                    <  2:844247/143‑1 (MQ=255)

                                                                              attcTTTTCCCTCTTTCGTAGTAATTAGCTTCTTTTTTTGCAACAGTAAAATTGCGCGACCTAGGTTGTTCGGGTTGTTGTGGATCGCTATTGTCGCGCCTTCTTTTATTTGCGCTACGGTTTTGATTTTTTTGGAATAAccc                                                                  <  2:590129/140‑1 (MQ=255)

                                                                               cccTTTTCCCTCTTTCAGAGTAATTAGCTTCTCTTTTTGCAACAGTAAAAGTGCGCGACCAAGGTTGGTCGGGTCGTTGGGGATCGCCACTGTCGCGCCTTCTTTTATTTGCGCCACGGTTTTGATTTTTTTGGAATCACCCg                                                                 >  1:524841/1‑143 (MQ=255)

                                                                                ccTTTTCCCTCTTTCAGAGTAATTAGCTTCTCTTTTTGCAACAGTAAAAGTGCGCGACCAAGGTTGGTCGGGTCGTTGGGGATCGCCACTGTCGCGCCTTCTTTTATTTGCGGCACGGTTTTGTGTTTTTTGGCTTATCCCGc                                                                >  2:1195488/1‑143 (MQ=255)

                                                                                ccTTTTCCCTCTTTCAGAGTAATTAGCTTCTCTTTTTGCAACAGTAAAAGTGCGCGACCAAGGTTGGTCGGGTCGTTGGGGATCGCCACTGTCGCGCCTTCTTTTATTTGCGCCACGGTTTTGATTTTTTTGGAATAACCCGc                                                                <  1:1163504/143‑1 (MQ=255)

                                                                                ccTTCTCCCTCTTTCAGAGGAATTAGCGTCTCTTTTTGCAACAGTAAAAGTGCGCGACCAAGGTTGGTCGGGTCGTTGGGGATCGCCACTGTCGCGCCTTCTTTTATTTGCGCCACGGTTTTGATTTTTTTGGAATAACCCGc                                                                <  1:381561/143‑1 (MQ=255)

                                                                                    ttCCCTCTTTCAGAGTAATTAGCTGCTCTTTTTGCAACAGTAAAAGTGCGCGACCAAGGTTGGTCGGGTCGTTGGGGATCGCCACTGTCGCGCCTTCTTTTATTTGCGCCACGGTTTTGATTTTTTTGGAATAACCCGCCAT‑a                                                           <  1:887515/143‑1 (MQ=255)

                                                                                         tctTTCAGAGTAATTAGCTTCTCTTTTTGCAGCAGTAAAAGTGCGCGACCAAGGTTGGTCGGGTCGTTGGGGATCGCCACTGTCGCGCCTTCTTTTATTTGCGCCACGGTTTTGATTTTTTTGGAATAACCCGCCAT‑AGGGaa                                                      <  2:1062032/143‑1 (MQ=255)

                                                                                         tctTTCAGAGTAATTAGCTTCTCTTTTTGCAACAGTAAAAGTGCGCGACCAAGGTTGGTCGGGTCGTTGGGGATCGCCACTGTCGCGCCTTCTTTTATTTGCGCCACGGTTTTGATTTTTTTGGAATAACCCGCCAT‑AGGGaa                                                      <  1:677391/143‑1 (MQ=255)

                                                                                         tctTTCAGAGTAATTAGCTTCTCTTTTTGCAACAGTAAAAGTGCGCGACCAAGGTTGGTCGGGTCGTTGGCGATCGCCACTGTCGCGCCTTCTTGTATTTGCGCCACGGTTTTGATTTTTTTGGAATAACCCGCCAT‑AGGGaa                                                      >  2:840852/1‑143 (MQ=255)

                                                                                            ttCAGAGTAATTAGCTTCTCTTTTTGCAACAGTAAAAGTGCGCGCCCAAGGTTGGTCGGGTCGTTGGGGATCGCCACTGTCGCGCCTTCTTTTATTTGCGCCACGGTTTTGGCTTTTTTGGCATAACCCGCCAT‑AGGGATGAc                                                   >  2:321304/1‑143 (MQ=255)

                                                                                            ttCAGAGTAATTAGCTTCTCTTTTTGCAACAGTAAAAGTGCGCGACCAAGGTTGGTCGGGTCGTTGGGGATCGCCACTGTCGCGCCTTCTTTTATTTGCGCCACGGTTTTGATATTTTTGGACTAACCCGCCAT‑Agtgcggag                                                   >  2:863447/1‑136 (MQ=255)

                                                                                               agagTAATTAGCTTCTCTTTTTGCAACAGTAAAAGTGCGCGACCAAGGTTGGTCGGGTCGTTGGGGATCGCCACTGTCGCGCCTTCTTTTATTTGCGCCACGGTTTTGATTTTTTTGGAATAACCCGCCAT‑AGGGAAGACaaa                                                >  2:285111/1‑143 (MQ=255)

                                                                                                gagTAATTAGCTTCTCTTTTTGCAACAGTAAAAGTGCGCGACCAAGGTTGGTCGGGTCGTTGGGGATCGCCACTGTCGCGCCTTCTTTTATTTGCGCCACGGTTTTGATTTTTTTGGAATAACCCGCCAT‑AGGGAAGACAAAc                                               <  1:141352/143‑1 (MQ=255)

                                                                                                 agTAATTAGCTTCTCTTTTTGCAACAGTAAAAGTGCGCGACCAAGGTTGGTCGGGTCGTTGGGGATCGCCACTGTCGCGCCTTCTTTTATTTGCGCCACGGTTTTGATTGTTTTGGAATAACCCGCCAT‑AGGGAAGACAAACg                                              >  1:430094/1‑143 (MQ=255)

                                                                                                        aGCTTCTCTTTTTGCAACAGTAAAAGTGCGCGACCAAGGTTGGTCGGGTCGTTGGGGATCGCCACTGTCGCGCCTTCTTTTATTTGCGCCACGGTTTTGATTTTTTTGGAATAACCCGCCAT‑AGGGAAGACAAACGTATTTcc                                       <  2:244229/143‑1 (MQ=255)

                                                                                                          cTTCTCTTTTTGCAACAGTAAAAGTGCGCGACCAAGGTTGGTCGGGTCGTTGGGGATCGCCACTGTCGCGCCTTCTTTTATTTGCGCCACGGTTTTGATTTTTTTGGAATAACCCGCCAT‑AGGGAATGCAAACGTATTTctct                                     >  2:1184838/1‑140 (MQ=255)

                                                                                                          cTTCTCTTTTTGCAACAGTAAAAGTGCGCGACCAAGGTTGGTCGGGTCGTTGGGGATCGCCACTGTCGCGCCTTCTTTTATTTGCGCCACGGTTTTGATTTTCTTGGACTAACCCGCCAT‑AGGGAAGACAAACGTATTTTCCa                                     >  2:583377/1‑143 (MQ=255)

                                                                                                           gtctctcTTTCCAAGATTACAAGTGCGCAACCAAGGTTGGTCGGGTCGTTGGGGATCGCCACTGTCGCGCCTTCTTTTATTTGCGCCACGGTTTTGATTTTTTTGGAATAACCCGCCAT‑AGGGAAGACAAACGTATTTCCCAc                                    <  1:351922/142‑1 (MQ=255)

                                                                                                             ctctTTTTGCAACAGTAAAAGTGCGCGACCAAGGTTGGTCGGGTCGTTGGGGATCGCCACTGTCGCGCCTTCTTTTATTTGCGCCACGGTTTTGATTTTTTTGGAATAACCCGCCAT‑AGGGAAGACAAACGTATTTCCCACCg                                  >  1:1142579/1‑143 (MQ=255)

                                                                                                              tctTTTTGCAACAGTAAAAGTGCGCGACCAAGGTTGGTCGGGTCGTTGGGGATCGCCACTGTCGCGCCTTCTTTTATTTGCGCCACGGTTTTGATTTTTTTGGAATAACCCGCCAT‑AGGGAAGGCAAACGTATTTCCCACCGc                                 >  1:793640/1‑143 (MQ=255)

                                                                                                               ctTGTTGCAACAGTAAAAGTGCGCGACCCAGGTTGGTCGGGTCGTTGGGGATCGCCACTGTCGCGCCTTCTTTTATTTGCGCCACGGTTTTGATTTTTTTGGAATAACCCGCCAT‑AGGGAAGACAAACGTATTTCCCACCGCt                                <  2:684735/143‑1 (MQ=255)

                                                                                                                 ttttGCAACAGTAAAAGTGCGCGACCAAGGTTGGTCGGGTCGTTGGGGATCGCCACTGTCGCGCCTTCTTTTATTTGCGCCACGGTTTTGATTTTTTTGGAATAACCCGCCAT‑AGGGAAGACAAACGTATTTCCCACCGCTAc                              <  1:115381/143‑1 (MQ=255)

                                                                                                                  tttGCAACAGTAAAAGTGCGCGACCAAGGTTGGTCGGGTCGTTGGGGATCGCCACTGTCGCGCCTTCTTTTATTTGCGCCACGGTTGTGATTTTTTTGGACTAACCCGCCAT‑AGGGAAGACAAACGGATTTCCAACCGCTAcc                             >  2:72416/1‑143 (MQ=255)

                                                                                                                   ttGCAACGGTAAAAGTGCGCGACCAAGGTTGGTCGGGTCGTTGGGGATCGCCACTGTCGCGCCTTCTTTTATTTGCGCCACGGTTTTGATTTTTTTGGAATAACCCGCCAT‑AGGGAAGACAAACGTATTTCCCACCGCTACCa                            <  2:164816/143‑1 (MQ=255)

                                                                                                                       aaCAGTAAAAGTGCGCGACCAAGGTTGGTCGGGTCGTTGGGGATCGCCACTGTCGCGCCTTCTTTTATTTGCGCCACGGTTTTGATTTTTTTGGAATAACCCGCCAT‑AGGGAGGACAAACGTATTTCCCACCGCTACGAGttt                        >  1:1259384/1‑143 (MQ=255)

                                                                                                                        aCAGTAAAAGTGCGCGACCAAGGTTGGTCGGGTCGTTGGGGATCGCCACTGTCGCGCCTTCTTTTATTTGCGCCACGGTTTTGCTTTTTTTGGAATAACCTCTGCT‑CAGCAAGACAGACGTATTTCCTACCGCTACCAGTTta                       >  2:562055/1‑143 (MQ=255)

                                                                                                                            tAAAAGTGCGCGACCAAGGTTGGTCGGGTCGTTGGGGATCGCCACTGTCGCGCCTTCGTTTATTTGCGCCCCGGTTTTGATTTTTTTGGAATAACCCGCCAT‑CGGGAAGACAAACGTATTTCCCACCGCTACCAGTTTATAAc                   >  1:630565/1‑143 (MQ=255)

                                                                                                                               tggTGCGCGACCGAGGTTGGGCGGGTCGTTGGGGATCGCCACTGTCGCGCCTTCTTTTATTTGCGCCACGGTTTTGATTTTTTTGGAATAACCCGCCAT‑AGGGAAGACAAACGTATTTCCCACCGCTACCAGTTTATAACCAt                <  2:378549/141‑1 (MQ=255)

                                                                                                                               aaGTGCGCGAGCAAGCTTGGTCGGGTCTTTTGGGATCTCCTCTGTCCCGCCTTCTTTTATTTGCGCCACGGTTTTGATTTTTTTGGAATAACCCGCCAT‑AGGGAAGACAAACGTATTTCCCACCGCTACCAGTTTATAACCAt                <  2:1274035/143‑1 (MQ=255)

                                                                                                                               aaGTGCGCGACCAAGGTTGGTCGGGTCGTTGGGGATCGCCACTGTCGCGCCTTCTTTTATTTGCGCCACGGTTTTGATTTTTTTGGAATAACCCGCCAT‑AGGGAAGACAAACGTATTTCCCACCGCTACCAGTTTATAACCAt                <  1:262188/143‑1 (MQ=255)

                                                                                                                                aGTGCGCGGCCCAGGTTGGTCGGGTCGTTGGGGATCGCCACTGTCGCGCCTTCTTTTATTTGCGCCACGGTTTTGATTTTTTTGGAATAACCCGCCAT‑AGGGAAGACAAACGTATCTCCCACCGCTACCAGTTTATAACCATg               <  2:778310/143‑1 (MQ=255)

                                                                                                                                aGTGCGCGACCAAGGTTGGTCGGGTCGTTGGGGATCGCCACTGTCGCGCCTTCTTTTATTTGCGCCACGGTTTTGATTTTTTTGGAATAACCCGCCAT‑AGGGAAGACGAACGTCTTTCCCACCGCTACCAGTTTATAAGCATg               >  1:1006567/1‑143 (MQ=255)

                                                                                                                                aGTGCGCGACCAAGGTTGGTCGGGTCGTTGGGGATCGCCACTGTCGCGCCTTCTTTTATTTGCGCCACGGTTTTGATTTTTTTGGAATAACCCGCCAT‑AGGGAAGACAAACGTATTTCCCACCGCTACCAGTTTATAACCATg               >  1:608602/1‑143 (MQ=255)

                                                                                                                                  tGCGCGACCAAGGTTGGTCGGGTCGTTGGGGATCGCCACTGTCGCGCCTTCTTTTATTTGCGCCACGGTTTTGATTTTTTTGGAATAACCCGCCAT‑AGGGAAGACAAACGTATTTCCCACCGCTACCAGTTTATAACCATgcg             >  1:204134/1‑143 (MQ=255)

                                                                                                                                    cccgACCAACCTTGGTCGGGTCGTTGGGGATCGCCACTGTCGCGCCTTCTTTTATTTGCGCCACGGTTTTGATTTTTTTGGAATAACCCGCTAT‑AGGGAAGACAAACGTATTTCCCACCGCTACCCGTTTATAACCAttcccc           >  2:911357/3‑138 (MQ=255)

                                                                                                                                    cccgACCAACCTTGGTCGGGTCGTTGGGGATCGCCACTGTCGCGCCTTCTTTTATTTGCGCCACGGTTTTGATTTTTTTGGAATAACCCGCCAT‑AGGGCAGCAAACCGCTTTTCCCACCGGTACCCGTCTATCACCATgcggc           >  1:232695/3‑141 (MQ=255)

                                                                                                                                    cccgACCAACCTTGGTCGGGTCGTTGGGGATCGCCACTGTCGCGCCTTCTTTTATTTGCGCCACGGTTTTGA‑TTTTTTGGAATAACCCGCCATAAGTGAAG‑CAAACGTAGTTCCCACCGCCACCAGATTATAACCATGCGACt          >  2:710571/3‑143 (MQ=255)

                                                                                                                                      cgACCAGGGGTGGTTGGGTCGTTGGGGATCGCCACTGTCGCGCCTTCTGTTATTTGCGCCAGGGTTTTGATTTTTTTGGAATAACCCGCCCT‑AGGGAAGACAAACGTATTTCTCACCGCTACCAGTTTATAACCATGCGCCTg         <  2:161755/143‑1 (MQ=255)

                                                                                                                                       gACCAAGGTTGGTCGGGTCGTTGGGGATCGCCACTGTCGCGCCTTCTTTTATTTGCGCCACGGTTTTGATTTTTTTGGAATAACCCGCCAT‑AGGGAAGACAAACGTATTTCCCACCGCTACCAGTTTATAACCATGCGCCTGa        >  1:621312/1‑143 (MQ=255)

                                                                                                                                           aaGGGTGGTCGGGTCGTTGGGGATCGCCACTGTCGCGCCTTCTTTTATTTGCGCCACGGTTTTGATTTTTTTGGAATAACCCGCCAT‑AGGGAAGACAAACGTATTTCCCACCGCTACCAGTTTATAACCATGCGCCTGATTAt    <  1:510224/143‑1 (MQ=255)

                                                                                                                                            aGGTTGGTCGGGTCGTTGGGGCTCGCCACTGTCGCGCCTTCTTTTATTTGCGCCACGGTTTTGATTTTTTTGGAATAACCCGCCAT‑AGGGAAGACAAACGTATTTCCCACCGCTACCAGTTTATAACCATGCGCCTGATTATc   <  1:1049522/143‑1 (MQ=255)

                                                                                                                                            aGGTGGGTCGGGTCGTTGGGGATCGCCACTGTCGCGCCTTCTTTTATCTCCGCCACTTTTTTGATTTTTTTGGAATACCCCGCCAT‑AGGGAAGACAAACGTATTTCCCACCGCTACCAGTTTATAACCATGCGCCTGATTATc   <  1:412293/143‑1 (MQ=255)

GCGGGAGCTGCGCCCCCTCGAGTTCCATAATCTGCAAATGACGAGGATTATCGGTTATATCCAGCGCGGTAGGTAATAACCCTTTTCCCTCTTTCAGAGTAATTAGCTTCTCTTTTTGCAACAGTAAAAGTGCGCGACCAAGGTTGGTCGGGTCGTTGGGGATCGCCACTGTCGCGCCTTCTTTTATTTGCGCCACGGTTTTGATTTTTTTGGAATAACCCGCCAT‑AGGGAAGACAAACGTATTTCCCACCGCTACCAGTTTATAACCATGCGCCTGATTATCT  >  NC_000913/3839426‑3839709

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 8 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`