Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,041,398	T→G	L29R (CTT→CGT)	ygfZ →	folate‑binding protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,041,398	0	T	G	98.0%	159.9 / NA	51	L29R (CTT→CGT)	ygfZ	folate‑binding protein
Reads supporting (aligned to +/- strand):  ref base T (0/0);  major base G (24/26);  minor base A (1/0);  total (25/26)
Fisher's exact test for biased strand distribution p-value = 4.90e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.97e-01

AGCTCTTTTACCATTAGTCATTAATACGCCGTTAAGCAACTCAGGACTCTATTATGGCTTTTACACCTTTTCCTCCCCGTCAGCCTACGGCTTCTGCCCGTTTACCGCTGACGCTGATGACGCTTGATGACTGGGCGCTTGCCACCATTACTGGCGCGGACAGCGAAAAATATATGCAGGGTCAGGTGACAGCAGATGTCAGCCAGATGGCAGAAGATCAGCACCTGCTCGCCGCCCATTGCGACGCCAAAGGTAAAATGTGGAGCAATTTACGTCT  >  NC_000913/3041260‑3041536                                                                                                                                           |                                                                                                                                           ggCTCTGTTCCCATTAGTCATTAATAGGCCGTTGAGCAACTCAGGACTCTATTATGGCTCTTACACCTTTTCCCCCCCGTCAGCCTACGGCTTCTGCCCGTTTACCGCTGACGCTGATGACGCTTGATGACTGGGCGCGTGcc                                                                                                                                        <  2:641623/142‑1 (MQ=255)   ctctTTTACCATTAGTCATTAATACGCCGTTAAGCAACTCAGGACTCTATTATGGCTTTTACACCTTTTCCTCCCCGTCAGCCTACGGCTTCTGCCCGTTTACCGCTGACGCTGATGACGCTTGATGACTGGGCGCGTGccac                                                                                                                                      >  1:783482/1‑143 (MQ=255)            cgttAGTCATTAATACGCCGTTAAGCAACTCAGGACTCTATTATGGCTTTTACACCTTTTCCTCCCCGTCAGCCTACGGCTTCTGCCCGTTTACCGCTGACTCTGATGACGCTTGATGACTGGGCGCGTGCCACCATTACTgg                                                                                                                             <  1:587279/141‑1 (MQ=255)            cATTAGTCATTAATACGCCGTTAAGCAACTCAGGACTCTATTATGGCTTTTACACCTTTTCCTCCCCGTCAGCCTACGGCTTCTGCCCGTTTACCGCTGACTCTGATGACGCTTGATGACTGGGCGCGTGCCACCATTACTgg                                                                                                                             <  1:587651/143‑1 (MQ=255)            cATTAGTCATTAATACGCCGTTAAGCAACTCAGGACTCTATTATGGCTTTTACACCTTTTCCTCCCCGTCAGCCTACGGCTTCTGCCCGTTTACCGCTGACGCTGATGACGCTTGATGACTGGGCGCGTGccacca                                                                                                                                    <  2:435695/136‑1 (MQ=255)            cATTAGTCATTAATACGCCGTTAAGCAACTCAGGACTCTATTATGGCTTTTACACCTTTTCCTCCCCGTCAGCCTACGGCTTCTGCCCGTTTACCGCTGACGCTGATGACGCTTGATGACTGGGCGCGTGccacca                                                                                                                                    >  1:435695/1‑136 (MQ=255)            cATTAGTCATTAATACGCCGTTAAGCAACTCAGGACTCTATTATGGCTTTTACACCTTTTCCTCCCCGTCAGCCTACGGCTTCTGCCCGTTTACCGCTGACGCTGATGACGCTTGATGACTGGGCGCGTGCCCCCATTACCgg                                                                                                                             >  2:520315/1‑143 (MQ=255)            cATTAGTCATTAATACGCAGTTAAGCAACTCAGGACTCTATTATGGCTTTTCCACCTTTTCCTCACCGTCAACCTACGGCTTCTGCCCGTTTACCGCTGACGCTGATGACACTTGATGACGGGGCGCGTGCCACCATTAccga                                                                                                                             >  2:303670/1‑140 (MQ=255)               tAGTGATTAAGACGCCGTTAAGCAACTCAGGACTCTATTATGGCGTTTACACCTTTTCCTCCCCGTCAGCCTACGGCTTCTGCCCGTTTACCGCTGACGCTTATGACGCTTGATGACTGGGCGCGTGCCACCATTACTGgcgc                                                                                                                          <  1:37175/143‑1 (MQ=255)               tAGTCATTAATACGCCGTTAAGCAAGTCAGGACTCTATTATGGCTTTTACACCTTTTCCTCCGCGTCAGCCTACGGCTTCTGCCCGTTTACCGCTGACGCTGATGACGCTTGATGACTGGGCGCGTGCCACCATTACTGgcgc                                                                                                                          <  1:448767/143‑1 (MQ=255)                 gTCATTAATACGCCGTTAAGCAACTCAGGACTCTATTATGGCTTTTACACCTTTTCCTCCCCGTCACCCTACGGCTTCTGCCCGTTTACCGCTGACGCTGATGACGCCTGATGACTGGGCGCGTGCCACCATTACTGGCACgg                                                                                                                        >  1:845405/1‑143 (MQ=255)                  tCATTTATACGCCGTTAAGCCTCTCAGGACTCTATTGTGGCTTTTACACCTTTTCCTCCCCGTCAGCCGACGGCGTCTGCCCGTTTCCCGCTGACGCTGATGACGCTTGATGACTGGGCGCGTGCCACCATTACTGGCGCGGa                                                                                                                       <  2:588090/143‑1 (MQ=255)                    aTTAATACGCCGTTAAGCAACTCAGGACTCTATTATGGCTTTTACACCTTTTCCTCCCCGTCAGCCTACGGCTTCTGCCCGTTTACCGCTGACGCTGATGACGCTTGATGACTGGGCGCGTGCCACCATTACTGGCGCGGACa                                                                                                                     <  2:783482/143‑1 (MQ=255)                    aTTAATACGCCGTTAAGCAACTCAGGACTCTATTATGGCTTTTACACCTTTTCCTCCCCGTCAGCCTACGGCTTCTGCCCGTTTACCGCTGACGCTGATGACGCTTGATGACTGGGCGCGTGCCACCATTACTGGCGCGGACa                                                                                                                     >  2:593817/1‑143 (MQ=255)                                      aaCTCAGGACTCTATTATGGCTTTTACGCCATTGCCTCCCCGTCAGCCTACGGCTTCTGCCCGTTTACCGCTGACGCTGATGACGCTTGATGACTGGGCGCGTGCCACCATTACTGGCGCGGACAGCGAAAAATATATGCAgg                                                                                                   <  1:160719/143‑1 (MQ=255)                                          cAGGACTCTATTATGGCTTTTACACCTTTTCCTCCCCGTCAGCCTACGGCTTCTGCCCGTTTACCGCTGACGCTGATGACGCTTGATGACTGGGCGCGTGCCACCATTACTGGCGCGGACAGCGAAAAATATATGCAGGGTCa                                                                                               <  2:559970/143‑1 (MQ=255)                                           aGGACTCTATTATGGCTTTTACACCTTTTCCTCCCCGTCAGCCTACGGCTTCTGCCCGTTTACCGCTGACGCTGATGACGCTTGATGACTGGGCGCGTGCCACCATTACTGGCGCGGACAGCGAAAATTATATGCAGGGTAAg                                                                                              >  1:521178/1‑143 (MQ=255)                                                tctATTATGGCTTTTACACCTTTTCCTCCCCGTCAGCCTACGGCTTCTGCCCGTTTACCGCTGACGCTGATGACGCTTGATGACTGGGCGCGTGCCACCATTACTGGCGCGGACAGCGAAAAATATATGCAGGGTCAGGTGAc                                                                                         >  1:704287/1‑143 (MQ=255)                                                 ctATTATGGGTTTTACACCTTTTCCTCCCCGTCAGCCTACGGCTTCTGCCCGTTTACCGCTGACGCTGATGACGCTTGATGACTGGGCGCGTGCCACCATTACTGGCGCGGACAGCGAAAAATATATGCAGGGTCAGGTGAca                                                                                        <  1:390582/143‑1 (MQ=255)                                                  tattatGGCTTTTACACCTTTTCCTCCCCGTCAGCCTACGGCTTCTGCCCGTTTACCGCTGACGCTGATGGCGCTTGATGACTGGGCGCGTGCCACCATTACTGGCGCGGACAGCGAGAAATATATGCAGGGTGAGGTGAcag                                                                                       >  1:797215/1‑143 (MQ=255)                                                     tgtGGCTGTTACACCTTTGCCGCCCCGTCAGCCTACGGCTGCTGCCCGTTTACCGCTGACGCTGATGACGCTTGATGACTGGGCGCGTGCCACCATTACTGGCGCGGACAGCGAAAAATATATGCAGGGTCAGGTGAcagcag                                                                                    <  1:546726/141‑1 (MQ=255)                                                     tatGTCTTTGACACCTTTTCCTCCCCGTCAGCCTACGGCTTCTGCCCGTTTACCGCTGACGCTGATGACGCTTGATGACTGGGCGCGTGCCACCATTACTGGCGCGGACAGCGAAAAATATATGCAGGGTCAGGTGAcagcag                                                                                    <  1:494906/143‑1 (MQ=255)                                                           ttttACACCTTTTCCTCCCCGTCAGCCTACGGCTTCTGCCCGTTTACCGCTGACGCTGATGACGCTTGATGACTGGGCGCGTGCCACCATTACTGGCGCGGACAGCGAAAAATATATGCAGGGTCAGGTGACAGCAGATGTCa                                                                              <  1:196023/143‑1 (MQ=255)                                                                cacCTTTTGCTCCCCGTCAGCCTACGGCTTCTGCCCGTTCACCGCTGACGCTGATGATGCTTGATGACTGGGCGCGTGCCACCATTACTGGCGCGGACAGCGAAAAATATATGCAGGGTCAGGTGACAGCAGATGTCAGCCAg                                                                         <  2:539232/143‑1 (MQ=255)                                                                cacCTTTCCCTTCGCGTGTGCCTAGTGCTTCTGCCCGTTTACCCCTGACGCTGATGACGCTTGATGACTGGGCGCGTGCCACCATTACTGGCGCGGACAGCGAAAAATATATGCATGGTCAGGTGACAGCAGATGTCAGCCAg                                                                         <  2:347847/143‑1 (MQ=255)                                                                 cacctttCCTCCCCGTCAGCCTACGGCTTCTGCCCGTTTACCGCTGACGCTGATGACGCTTGATGACTGGGCGCGTGCCACCATTACTGGCGCGGACAGCGAAAAATATATGCAGGGTCAGGTGACAGCAGATGTCAGCCAGa                                                                        <  2:845380/139‑1 (MQ=255)                                                                   cTTTTCCTCCCCGTCAGCCTACGGCTTCTGCCCGTTTACCGCTGACGCTGATGACGCTTGATGACTGGGCGCGTGCCACCATTACTGGCGCGGACAGCGAAAAATATATGCAGGGTCAGGTGACAGCAGATGTCAGCCAGATg                                                                      >  1:564917/1‑143 (MQ=255)                                                                    ttttCCTCCCCGTCAGCCTACGGCTTCTGCCCGTTTACCGCTGACGCTGATGACGCTTGATGACTGGGCGCGTGCCACCATTACTGGCGCGGACAGCGAAAAATATATGCAGGGTCAGGTGACAGCAGATGTCAGCCAGATgg                                                                     >  1:450682/1‑143 (MQ=255)                                                                      ttCCTCCCCGTCAGCCTACGGCTTCTGCCCGTTTACCGCTGACGCTGATGACGCTTGATGACTGGGCGCGTGCCACCATTACTGGCGCGGACAGCGCCACCTCTATGTAGTGTCAGGTGACAGCAGATGTCAGCCAGATGGCa                                                                   <  1:611198/143‑1 (MQ=255)                                                                          tccCCGTCAGCCTACGGCTTCTGCCCGTTTACCGCTGACGCTGATGACGCTTGATGACTTGGCACAAGCCACCAATACTGGAGCGGACAGCGAAAAATATATGCAGGGTCAGGTGACAGCAGATGTCAGCCAGATGGCagaag                                                               >  1:465307/1‑143 (MQ=255)                                                                          tccCCGTCAGCCTACGGCTTCTGCCCGTTTACCGCTGACGCTGATGACGCTTGATGACTGGGCGCGTGCCACCATTACTGGCGCGGACAGCGAAAAATATATGCAGGGTCAg                                                                                              >  1:326466/1‑112 (MQ=255)                                                                          tccCCGTCAGCCTACGGCTTCTGCCCGTTTACCGCTGACGCTGATGACGCTTGATGACTGGGCGCGTGCCACCATTACTGGCGCGGACAGCGAAAAATATATGCAGGGTCAg                                                                                              <  2:326466/112‑1 (MQ=255)                                                                          tccCCGTCAGCCTACGGCTTCTGCCCGTTTACCGCTGACGCTGATGACGCTTGATGACTGGGCGCGTGCCACCATTACTGGCGCGGACAGCGAAAAATATATGCAGGGTCAGGTGACAGCAGATGTCAGCCAGATGGCaggag                                                               >  1:703449/1‑143 (MQ=255)                                                                             ccggccgCCTACGGCTTGCGCCCGTTTCCCGCTGACGCTGATGACGCTTGATGACTGGGCGCGTGCCACCATTACTGGCGCGGACAGCGAAAAATATATGCAGGGTCAGGTGACAGCAGATGTCAGCCAGATGGCAGAAGATc                                                            <  2:882067/137‑1 (MQ=255)                                                                             ccGTCAGCCTACGGCTTCTGGCCGGTTACCGCTGACGCTGATGACGCTTGATGACTGGGCGCGTGCCACCATTACTGGCGCGGACAGCGAAAAATATATGCAGGGTCAGGTGACAGCAGATGTCAGCCAGATGGCAGAAGATc                                                            <  2:454552/143‑1 (MQ=255)                                                                              cGTCAGCCTACGGCTTCTGCCCGTTTACCGCTGACGCTGATGACGCTTGATGACTGGGCGCGTGCCACCATTACTGGCGCGGACAGCGAAAAATATATGCAGGGTCAGGTGACAGCAGATGTCAGCCAGATGGCAGAAGATCa                                                           <  1:858100/143‑1 (MQ=255)                                                                                    ccTACGGCTTCTGCCCGTTTACCGCTGACGCTGATGACGCTTGATGACTGGGCGCGTGCCACCATTACTGGCGCGGACAGCGAAAAATATATGCAGGGTCAGGTGACAGCAGATGTCAGCCAGATGGCAGAAGATCAGcgact                                                     >  1:69463/1‑139 (MQ=255)                                                                                    ccTACGGCTTCTGCCCGTTTACCGCTGACGCTGATGACGCTTGATGACTGGGCGCGTGCCACCATTACTGGCGCGGACAGCGAAAAAGATATGCAGGGTCAGGTGACAGCAGATGTCAGCCAGATGGCAGAAGATCAGCAGCt                                                     >  1:94564/1‑143 (MQ=255)                                                                                     cTACGGCTTCTGCCCGTTTACCGCTGACGCTGATGACGCTTGATGACTGGGCGCGTGCCACCATTACTGGCGCGGACAGCGGAAAGTATATGCAGGGTCAGGTGACAGCCGATGTCAGCCAGGTGGCAGAAGATCAGGACGTg                                                    >  1:389702/1‑143 (MQ=255)                                                                                            ttCTGCCCGTTTACCGCTGACGCTGATGACCCTTCATGACTGGTCGCGTGCCACCATTACTGGCGCGGACAGCTAAAAATATATGCAGGGTCAGGTGACAGCAGATGTCAGGCAGATGGCAGAAGATCAGCACCTGCTcgccg                                             <  1:689036/143‑1 (MQ=255)                                                                                             gcTGCCCGTTTACCGCTGACGCTGATGACGCTTGATGACTGGGCGCGTGCCACCATTACTGGCGCGGACAGCGAAAAATATATGCAGGGTCAGGTGACAGCAGATGTCAGCCAGATGGCAGAAGATCAGCACCTGCTcgccgc                                            <  1:619505/142‑1 (MQ=255)                                                                                                  ccGTTTACCGCTGACGCTGATGACGCTTGATGACTGGGCGCGTGCCACCATTACTGGCGCGGACAGCGAAAAATATATGCAGGGTCAGGTGACAGCAGATGTCACCCCGATGGCCGAAGCTCAGCACCTACTCGTCGACCAtt                                       >  1:190680/1‑143 (MQ=255)                                                                                                            ctgacgctgaTGACGCTTGATGACTGGGCGCGTGCCACCATTACTGGCGCGGACAGCGAAAAATATATGCAGGGTCAGGTGACAGCAGATGTCAGCCAGATGGCAGAAGATCAGCACCTGCTCGCCGCCCATTGCGACGCCag                             >  1:876389/1‑142 (MQ=255)                                                                                                             tgacgctgaTGACGCTTGATGACTGGGCGCGTGCCACCATTACTGGCGCGGAGAGCGAAAAATATATGCAGGGTCAGGTGACAGCAGATGTCAGCCAGATGGCAGAAGATCAGCACCTGCTCGCCGCCCCTTGCGACGGCaac                            >  1:258027/1‑142 (MQ=255)                                                                                                              gacgctgaTGACGCTTGATGCCTGGGCGCGTGCCACCATTACTGGCGCGGACAGCGAAAAATATATGCAGGGTCAGGTGACAGCAGATGTCAGCCAGATGGCAGAAGATCAGCACCTGCTCGCCGCCCATTGCGACGCCAAAg                           <  1:892598/143‑1 (MQ=255)                                                                                                              gacgctgaTGACGCTTGATGACTGGGCGCGTGCCACCATTACTGGCGCGGACAGCGAAAAATATATGCAGGGTCAGGTGACAGCAGATGTCAGCCAGATGGCAGAAGATCAGCACCTGCTCGCCGCCCATGGCGACGCCAAAg                           >  2:487578/1‑143 (MQ=255)                                                                                                                   tgatgaCGCTTGATGACTGGGCGCGTGCCACCATTACTGGCGCGGACAGCGAAAAATATATGCAGGGTCAGGTGACAGCAGATGTCAGCCAGATGGCAGAAGATCAGCACCTGCTCGCCGCCCATTGCGACGCCAAAGGTaaa                      >  1:657611/1‑143 (MQ=255)                                                                                                                        aCGCTGGATGACTGGGCGCGTGCCACCATTACTGGCGCGGACAGCGAAAAATATATGCAGGGTCAGGTGACAGCAGATGTCAGCCAGATGGCAGAAGATCAGCACCTGCTCGCCGCCCATTGCGACGCCAAAGGTAAAAtgtg                 <  1:874079/143‑1 (MQ=255)                                                                                                                              gatgaCTGGGCGCGTGCCACCATTACTGGCGCGGACAGCGAAAAATATATGCAGGGTCAGGTGACAGCAGATGTCAGCCAGATGGCAGAAGATCAGCACCTGCTCGCCGCCCATTGCGACGCCAAAGGTAAAACGTGGAGCac           >  1:539208/1‑142 (MQ=255)                                                                                                                               atgaCTGGGCGCGTGCCACCATTACTGGCGCGGACAGCGAAAAATATATGCAGGGTCAGGTGACAGCAGATGTCAGCCAGATGGCAGAAGATCAGCACCTGCTCGCCGCCCATTGCGACGCCAAAGGTAAAATGTGGAGCAAt          >  1:743261/1‑143 (MQ=255)                                                                                                                                       gcgcGTGCCACCATTACTGGCGCGGACAGCGAAAAATATATGCAGGGTCAGGTGACAGCAGATGTCAGCCAGATGGCAGAAGATCAGCACCTGCTCGCCGCCCATTGCGACGCCGAAGGTAAAATGTGGAGCAATTTACGTCt  >  1:191592/1‑143 (MQ=255)                                                                                                                                           |                                                                                                                                           AGCTCTTTTACCATTAGTCATTAATACGCCGTTAAGCAACTCAGGACTCTATTATGGCTTTTACACCTTTTCCTCCCCGTCAGCCTACGGCTTCTGCCCGTTTACCGCTGACGCTGATGACGCTTGATGACTGGGCGCTTGCCACCATTACTGGCGCGGACAGCGAAAAATATATGCAGGGTCAGGTGACAGCAGATGTCAGCCAGATGGCAGAAGATCAGCACCTGCTCGCCGCCCATTGCGACGCCAAAGGTAAAATGTGGAGCAATTTACGTCT  >  NC_000913/3041260‑3041536

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑