Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,196,220	C→T	H366H (CAC→CAT)	icd →	isocitrate dehydrogenase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,196,220	0	C	T	96.0%	48.7 / NA	27	H366H (CAC→CAT)	icd	isocitrate dehydrogenase
Reads supporting (aligned to +/- strand):  ref base C (1/0);  major base T (18/6);  minor base G (0/1);  total (20/7)
Fisher's exact test for biased strand distribution p-value = 2.80e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.87e-01

GTATCGCCCCTGGTGCAAACATCGGTGACGAATGCGCCCTGTTTGAAGCCACCCACGGTACTGCGCCGAAATATGCCGGTCAGGACAAAGTAAATCCTGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCCACATGGGTTGGACCGAAGCGGCTGACTTAATTGTTAAAGGTATGGAAGGCGCAATCAACGCGAAAACCGTAACCTATGACTTCGAGCGTCT  >  NC_000913/1196084‑1196309                                                                                                                                         |                                                                                          gtatcgCCCCTGGTGCAAACATCGGTGACGAATGCGCCCTGTTTGCAGCCACCCACGGTACTGCGCCGAAATATGCCGGTCAGGACAAAGTAAATCCTGGCTCTCTTATTCTCTCCGCTGAGATGATGCTGCGCCATATGGGt                                                                                     >  1:29588/1‑143 (MQ=255) gtatcgCCCCTGGTGCAAACAGCGGTGGCGAATGCGCCGTGTGTGAAGCCACCCACGGTACTGCGCCGAAATCGGCCGGTGAAGACAGAATAAAGCCTTGATCGATTATTCTCGCCGCTTAGATGATGGTGCGCCATAggggg                                                                                     >  2:225927/1‑142 (MQ=255)   atcgCCCCTGGTGCAAACGTCGGTGACGAATGCGCCCTGTTTGATGCCACCCACGGTACTGCGCCGAAGTATGCCGGTCAGGACAAAGTAAAGCCTGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCCATATGGGTTg                                                                                   <  2:610828/143‑1 (MQ=255)          cTGGTGCAAACATCGGTGACGAATGCGCCCTGTTTGAAGCCACCCACGGTACTGCGCCGAAATATGCCGGTCAGGACAAAGTAAATCCTGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCCATATGGGTTGGACTGaa                                                                            >  1:239275/1‑143 (MQ=255)                        ggTGACGAATGCGCCCTGTTTGAAGGCACCCACGGTACTGCGCCGAAATATGCCGGTCAGGACAAAGTAAATCCTGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCCATATGGGTTGGACTGAAGCGGCTGAcctgat                                                              <  1:641612/143‑6 (MQ=38)                        ggTGACGAATGCGCCCTGTTTGAAGCCACCCACGGTACTGCGCCGAAATATGCCGGTCAGGACAAAGTAAATCCTGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCCATATGGGTTGGACTGAAGCGGCTGAcctgat                                                              <  1:212983/143‑6 (MQ=255)                                    gcCCTGTTTGAAGCCACCCACGGTACTGCGCCGAAATATGCCGGTCAGGACAAAGTAAATCCTGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCGATACGGGTTGGACTGAAGCGGCTGACCTGATTGAGAAAGGTAt                                                  >  2:611305/1‑143 (MQ=38)                                    gcCCTGTTTGAAGCCACCCACGGTACTGCGCCGAAATATGCCGGTCAGGACAAAGTAAATCCTGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCCATATGGGTTGGGCTGAAGCGGCTGACCTGCTTGTCAAAGGTAt                                                  >  1:702711/1‑143 (MQ=38)                                     cccTGTTTGAAGCCTCTCACGTTTCTGCTCCGAAATATGCCGGTCAGGACAAAGTAAATCCTGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCCATATGGGTTGGACTGAAGCGGCTGACCTGATTGTTAAAGGTATg                                                 <  1:710364/143‑1 (MQ=25)                                      ccTGTTTGAAGCCACCCACGGTACTGCGCCGAAATATGCCGGTCAGGACAAAGTAAATCCTGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCCATATGGGTTGGACTGAAGCGGCTGACCTGATTGTTAAAGGTATgg                                                >  1:387224/1‑143 (MQ=255)                                          tttGAAGCCACCCACGGTACTGCGCCGAAATATGCCGGTCAGGACAAAGTAAATCCTGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCCATATGGGTTGGACTGAAGCGGCTGACCTGATTGTTAAAGGTATGGAAgg                                            >  1:92103/1‑143 (MQ=255)                                          tttGAAGCCACCCACGGTACTGCGCCGAAATATGCCGGTCAGGACAAAGTAAATCCTGGCTCTATTATTCTCTCCGCTGAGATGATGCTGAGCCATATGGGTTGGACTGAAGACGCTGACCTGACTGATACTGGAATGGAAgg                                            >  2:304815/1‑143 (MQ=37)                                            tGAAGCCACCCACGGTACTGCGCCGAAATATGCCGGTCAGGACAAAGTAAATCCTGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCCAAAATTGGGTTGGACTGAAGCGGCTGACCTGATTGTTAAAGGTATGGAAGgcg                                          >  1:200897/1‑143 (MQ=37)                                                gCCACCCACGGTACTGCGCCGAAATATGCCGGTCAGGACAAACTAAACCCTGGCTCTATTATTCTCTCCGCTGAGATCATGATGCGACACATGGGTTGGACTCAAGCGGCTTACCTCAATGCTAAAGGTATCGAAGGCGCaac                                      >  2:545423/1‑142 (MQ=255)                                                              tGCGCCGAAATATGCCGGTCAGGACAAAGTACAACCTGTAGCTAATATTCTCTCCGCTGAGATGATGCTGCGCAATATGGGTTGGACTGACGCGGCTGACCTGATTGTTAAAGGTATGGAAGTAGCAATCAATGCCaagatcg                        >  2:397298/1‑138 (MQ=17)                                                              tGCGCCGAAATATGCCGGTCAGGACAAAGTAAATCCTGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCCATATGGGTTGGACTGAAGCGGCTGACCTGATTGGTAAAGGTATGGAAGGCGCAATCAATGCCAAGACCg                        >  1:252810/1‑143 (MQ=18)                                                                cgcCGAAATATGCCGGTCAGGACAAAGTAAATCCTGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCCATATGGGTTGGACGGAAGCGGCTGACCTGAGTGTTAAGGGTATGGAAGGCGCAATCAGTGCCAAGACCGTa                      >  1:356933/1‑143 (MQ=18)                                                                 gcctgagtatGCCGGTCAGGACAAAGTAAATCCTGGCTCTATTATTCTCGCCGCTGAGATGATGCGGCGCCAGAGGGGTTGGACTGCAGCGGCTGACCTGATTGTTAAAGGTATGGAAGGCGCAATCAATGCCAAGACCGTaa                     <  2:287278/136‑1 (MQ=17)                                                                 gcCGAAATATGCCGGTCAGGACAAAGTAAATCCTGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCCATATGGGTTGGACTGAAGCGGCTGACCTGATTGTTAAAGGTATGGAAGGCGCAATCAATGCCAAGACCGTaa                     >  2:680619/1‑143 (MQ=18)                                                                   cGAAATATGCCGGTCAGGACAAAGTAAATCCTGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCCATATGGGTTGGACTGAAGCGGCTGACCTGATTGTTAAAGGTCTGGTAGGCGCAATCAATGCCAAGACCGTAAct                   >  1:353338/1‑142 (MQ=18)                                                                   cGAAATATGCCGGTCAGGACAAAGTAAATCCTGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCCATATGGGTTGGACTGAAGCGGCTGACCTGATTGTTAAAGGTATGGAAGGCGCAATCAATACCAAGACCGTAAct                   >  1:614476/1‑142 (MQ=18)                                                                      aaTATGCCGGTCAGGGCAAAGTAAATCCTGGCTCTATCATTCTCTCCGCTGAGATGATGCTGCGCCATATGGGTTGGACTGAAGCGGCTGACCTGATTGTTAAAGGTATGGAAGGCGCAATCAATGCCAAGACCGTAACTTAt                <  1:119872/143‑1 (MQ=14)                                                                      aaTATGCCGGTCAGGACAGAGTGAAGCCTGGCTTTATTATGCTCTCCGCTGAGAGGATGCTGAGCCATATGGGTTGTACTGAAGCGTCTGACCTTATTGTTAAAGGTATGGAAGGCGCAATCAATGCCAAGACCGTAACTTAt                <  2:265552/143‑1 (MQ=14)                                                                          tGCCGGTCAGGACAAAGTAAATCCTGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCCATGTGGGTTGGACTGAAGCGGCTGACCTGATTGTTAAAGGTATGGAAGGCGCAATCAATGCCAAGACCGTAACTTATGACt            >  2:432158/1‑143 (MQ=17)                                                                            ccGGTCAGGACAAAGTAAATCCTGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCCATATGGGTTGGACTGAAGCGGCTGACCTGATTGTTAAAGGTATGGAAGGCGCAATCAATGCCAAGACCGTAACTTATGACTTc          >  1:418114/1‑143 (MQ=17)                                                                              ggTCAGGACAAAGTAAATCCTGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCCATATGGGTTGGACTGGCGCGGCTGACCTGATTGTTAAAGGTATGGAAAGCGCAATAAATGCCCAGACCGTAACTTATGACtacaa        >  2:337384/1‑139 (MQ=17)                                                                                    gACAAAGTAAATCCTGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCCATATGGGTTGGACTGAAGCGGCTGACCTGATTGTTAAAGGTATGGAAGGCGCAATCAATGCCAAGACCGTAACTTATGACTTCGAACGTCt  >  1:791664/1‑143 (MQ=11)                                                                                                                                         |                                                                                          GTATCGCCCCTGGTGCAAACATCGGTGACGAATGCGCCCTGTTTGAAGCCACCCACGGTACTGCGCCGAAATATGCCGGTCAGGACAAAGTAAATCCTGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCCACATGGGTTGGACCGAAGCGGCTGACTTAATTGTTAAAGGTATGGAAGGCGCAATCAACGCGAAAACCGTAACCTATGACTTCGAGCGTCT  >  NC_000913/1196084‑1196309

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑