Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,196,232	C→T	T370T (ACC→ACT)	icd →	isocitrate dehydrogenase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,196,232	0	C	T	95.8%	49.3 / NA	24	T370T (ACC→ACT)	icd	isocitrate dehydrogenase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  major base T (17/6);  minor base G (1/0);  total (18/6)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.96e-01

CTGGTGCAAACATCGGTGACGAATGCGCCCTGTTTGAAGCCACCCACGGTACTGCGCCGAAATATGCCGGTCAGGACAAAGTAAATCCTGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCCACATGGGTTGGACCGAAGCGGCTGACTTAATTGTTAAAGGTATGGAAGGCGCAATCAACGCGAAAACCGTAACCTATGACTTCGAGCGTCT  >  NC_000913/1196093‑1196309                                                                                                                                            |                                                                              cTGGTGCAAACATCGGTGACGAATGCGCCCTGTTTGAAGCCACCCACGGTACTGCGCCGAAATATGCCGGTCAGGACAAAGTAAATCCTGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCCATATGGGTTGGACTGaa                                                                            >  1:239275/1‑143 (MQ=255)               ggTGACGAATGCGCCCTGTTTGAAGGCACCCACGGTACTGCGCCGAAATATGCCGGTCAGGACAAAGTAAATCCTGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCCATATGGGTTGGACTGAAGCGGCTGAcctgat                                                              <  1:641612/143‑6 (MQ=38)               ggTGACGAATGCGCCCTGTTTGAAGCCACCCACGGTACTGCGCCGAAATATGCCGGTCAGGACAAAGTAAATCCTGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCCATATGGGTTGGACTGAAGCGGCTGAcctgat                                                              <  1:212983/143‑6 (MQ=255)                           gcCCTGTTTGAAGCCACCCACGGTACTGCGCCGAAATATGCCGGTCAGGACAAAGTAAATCCTGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCGATACGGGTTGGACTGAAGCGGCTGACCTGATTGAGAAAGGTAt                                                  >  2:611305/1‑143 (MQ=38)                           gcCCTGTTTGAAGCCACCCACGGTACTGCGCCGAAATATGCCGGTCAGGACAAAGTAAATCCTGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCCATATGGGTTGGGCTGAAGCGGCTGACCTGCTTGTCAAAGGTAt                                                  >  1:702711/1‑143 (MQ=38)                            cccTGTTTGAAGCCTCTCACGTTTCTGCTCCGAAATATGCCGGTCAGGACAAAGTAAATCCTGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCCATATGGGTTGGACTGAAGCGGCTGACCTGATTGTTAAAGGTATg                                                 <  1:710364/143‑1 (MQ=25)                             ccTGTTTGAAGCCACCCACGGTACTGCGCCGAAATATGCCGGTCAGGACAAAGTAAATCCTGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCCATATGGGTTGGACTGAAGCGGCTGACCTGATTGTTAAAGGTATgg                                                >  1:387224/1‑143 (MQ=255)                                 tttGAAGCCACCCACGGTACTGCGCCGAAATATGCCGGTCAGGACAAAGTAAATCCTGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCCATATGGGTTGGACTGAAGCGGCTGACCTGATTGTTAAAGGTATGGAAgg                                            >  1:92103/1‑143 (MQ=255)                                 tttGAAGCCACCCACGGTACTGCGCCGAAATATGCCGGTCAGGACAAAGTAAATCCTGGCTCTATTATTCTCTCCGCTGAGATGATGCTGAGCCATATGGGTTGGACTGAAGACGCTGACCTGACTGATACTGGAATGGAAgg                                            >  2:304815/1‑143 (MQ=37)                                   tGAAGCCACCCACGGTACTGCGCCGAAATATGCCGGTCAGGACAAAGTAAATCCTGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCCAAAATTGGGTTGGACTGAAGCGGCTGACCTGATTGTTAAAGGTATGGAAGgcg                                          >  1:200897/1‑143 (MQ=37)                                       gCCACCCACGGTACTGCGCCGAAATATGCCGGTCAGGACAAACTAAACCCTGGCTCTATTATTCTCTCCGCTGAGATCATGATGCGACACATGGGTTGGACTCAAGCGGCTTACCTCAATGCTAAAGGTATCGAAGGCGCaac                                      >  2:545423/1‑142 (MQ=255)                                                     tGCGCCGAAATATGCCGGTCAGGACAAAGTACAACCTGTAGCTAATATTCTCTCCGCTGAGATGATGCTGCGCAATATGGGTTGGACTGACGCGGCTGACCTGATTGTTAAAGGTATGGAAGTAGCAATCAATGCCaagatcg                        >  2:397298/1‑138 (MQ=17)                                                     tGCGCCGAAATATGCCGGTCAGGACAAAGTAAATCCTGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCCATATGGGTTGGACTGAAGCGGCTGACCTGATTGGTAAAGGTATGGAAGGCGCAATCAATGCCAAGACCg                        >  1:252810/1‑143 (MQ=18)                                                       cgcCGAAATATGCCGGTCAGGACAAAGTAAATCCTGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCCATATGGGTTGGACGGAAGCGGCTGACCTGAGTGTTAAGGGTATGGAAGGCGCAATCAGTGCCAAGACCGTa                      >  1:356933/1‑143 (MQ=18)                                                        gcctgagtatGCCGGTCAGGACAAAGTAAATCCTGGCTCTATTATTCTCGCCGCTGAGATGATGCGGCGCCAGAGGGGTTGGACTGCAGCGGCTGACCTGATTGTTAAAGGTATGGAAGGCGCAATCAATGCCAAGACCGTaa                     <  2:287278/136‑1 (MQ=17)                                                        gcCGAAATATGCCGGTCAGGACAAAGTAAATCCTGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCCATATGGGTTGGACTGAAGCGGCTGACCTGATTGTTAAAGGTATGGAAGGCGCAATCAATGCCAAGACCGTaa                     >  2:680619/1‑143 (MQ=18)                                                          cGAAATATGCCGGTCAGGACAAAGTAAATCCTGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCCATATGGGTTGGACTGAAGCGGCTGACCTGATTGTTAAAGGTCTGGTAGGCGCAATCAATGCCAAGACCGTAAct                   >  1:353338/1‑142 (MQ=18)                                                          cGAAATATGCCGGTCAGGACAAAGTAAATCCTGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCCATATGGGTTGGACTGAAGCGGCTGACCTGATTGTTAAAGGTATGGAAGGCGCAATCAATACCAAGACCGTAAct                   >  1:614476/1‑142 (MQ=18)                                                             aaTATGCCGGTCAGGGCAAAGTAAATCCTGGCTCTATCATTCTCTCCGCTGAGATGATGCTGCGCCATATGGGTTGGACTGAAGCGGCTGACCTGATTGTTAAAGGTATGGAAGGCGCAATCAATGCCAAGACCGTAACTTAt                <  1:119872/143‑1 (MQ=14)                                                             aaTATGCCGGTCAGGACAGAGTGAAGCCTGGCTTTATTATGCTCTCCGCTGAGAGGATGCTGAGCCATATGGGTTGTACTGAAGCGTCTGACCTTATTGTTAAAGGTATGGAAGGCGCAATCAATGCCAAGACCGTAACTTAt                <  2:265552/143‑1 (MQ=14)                                                                 tGCCGGTCAGGACAAAGTAAATCCTGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCCATGTGGGTTGGACTGAAGCGGCTGACCTGATTGTTAAAGGTATGGAAGGCGCAATCAATGCCAAGACCGTAACTTATGACt            >  2:432158/1‑143 (MQ=17)                                                                   ccGGTCAGGACAAAGTAAATCCTGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCCATATGGGTTGGACTGAAGCGGCTGACCTGATTGTTAAAGGTATGGAAGGCGCAATCAATGCCAAGACCGTAACTTATGACTTc          >  1:418114/1‑143 (MQ=17)                                                                     ggTCAGGACAAAGTAAATCCTGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCCATATGGGTTGGACTGGCGCGGCTGACCTGATTGTTAAAGGTATGGAAAGCGCAATAAATGCCCAGACCGTAACTTATGACtacaa        >  2:337384/1‑139 (MQ=17)                                                                           gACAAAGTAAATCCTGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCCATATGGGTTGGACTGAAGCGGCTGACCTGATTGTTAAAGGTATGGAAGGCGCAATCAATGCCAAGACCGTAACTTATGACTTCGAACGTCt  >  1:791664/1‑143 (MQ=11)                                                                                                                                            |                                                                              CTGGTGCAAACATCGGTGACGAATGCGCCCTGTTTGAAGCCACCCACGGTACTGCGCCGAAATATGCCGGTCAGGACAAAGTAAATCCTGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCCACATGGGTTGGACCGAAGCGGCTGACTTAATTGTTAAAGGTATGGAAGGCGCAATCAACGCGAAAACCGTAACCTATGACTTCGAGCGTCT  >  NC_000913/1196093‑1196309

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑