| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 3,560,455 | +G | pseudogene (151/758 nt) | glpR ← | DNA‑binding transcriptional repressor GlpR |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 3,560,455 | 1 | . | G | 92.3% | 71.2 / 0.6 | 26 | pseudogene (151/758 nt) | glpR | DNA‑binding transcriptional repressor GlpR |
| Reads supporting (aligned to +/- strand): ref base . (1/1); new base G (9/15); total (10/16) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
TCGAGCCATTGGGGATTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGAT‑GG‑C‑CAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTG > NC_000913/3560327‑3560585 | tCGAGCCATTGGGGATTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGAT‑GG‑CGCAGGATCAGGttt < 1:621282/143‑1 (MQ=255) cGAGCCATTGGGGATTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTGGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAGGGCAGCGCGGCACCGGCGTGATGGG‑G‑CAGGATCAGGtttt > 1:182621/1‑143 (MQ=255) ccATTGGGGATTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGAT‑GG‑CGCAGGATCAGGTTTTGCTc > 1:130213/1‑143 (MQ=255) tGCTCCGCCACTTTGCGGGCGATGCGCTCTGTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGAT‑GG‑CGCAGGATCAGGTTTTGCTCCGCCAGCTCAtt < 1:380929/143‑1 (MQ=255) tGCCCCGCCACCTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGAT‑GG‑CGCAGGATCAGGTTTTGCTCCGCCAGCTCAtt < 1:297322/143‑1 (MQ=255) tCCGCCGCTTTGCGGGCGACGCGCTCTTGTTCTTCGGTCCGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGAT‑GG‑CGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAg < 1:394981/143‑1 (MQ=255) aCTTTGCGGGCGTTGCGCTCGTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGAC‑GG‑CGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTcgcg < 2:32502/143‑1 (MQ=255) aCTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGAT‑GG‑CGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTcgcg > 1:337692/1‑143 (MQ=255) gggCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGAT‑GGAG‑CAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATa < 1:384206/143‑1 (MQ=255) tctTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGAT‑GG‑CGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGcc > 2:633536/1‑142 (MQ=255) tcGGGCTGGGTGGCCTTGCGCTGGGGCCACGGCGTGTTAACCGAACCGGAAGGCAGCGCCGCACCGCCATGAT‑GG‑CGCGGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGaa < 2:348279/143‑1 (MQ=255) gTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGAT‑GG‑CGCAGGATCAGGTTTTGCCCCGCCAGCGTGTTGAGGTCGCGGCGACTAGTCTGCGGGCTGACGGGGAGATg > 1:495496/1‑143 (MQ=255) gCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGAT‑GG‑CGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGATGTCGCGGCGCATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCtt < 2:291739/143‑1 (MQ=255) cGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGAT‑GG‑CGCAGGATCAGGTTTTGCTCCGCGAGCTCATTGAGGTCGCCGCGAATAGTCTGCGGGCTGACGGAGAAATGCGCTAGCAGGACTTc > 2:670444/1‑143 (MQ=255) gagcGTGCCGCGGCGTGTTAACCGAACAGGATGGCAGCGCTGCACCGCCATGAG‑GG‑CGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCg < 2:73531/140‑1 (MQ=255) gATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGAT‑GG‑CGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCg < 1:395811/143‑1 (MQ=255) aTCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGAT‑GG‑CGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGCAATGCTCTACCAGCTCTTCgg > 1:623316/1‑143 (MQ=255) gtTAACCGAGCTGGAAGGCAGCGCCGCACCGCCATGAT‑GG‑CGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCct < 1:152121/143‑1 (MQ=255) gcccGGACTGGTGGGCAGCCCCGCACCGCGATGAT‑GG‑CGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCGGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCctgct < 2:710199/141‑1 (MQ=255) ccGAACTGGAAGGCAGCGCCGCACCGCCAGGGT‑GG‑CGCAGGAGCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGt < 2:30040/143‑1 (MQ=255) cGAACTGGAAGGCAGCGCCGCACCGCCATGAT‑GG‑CGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTTACATAACCCTGCTGtt > 1:651714/1‑143 (MQ=255) tGGAAGGCAGCGCCGCACCGCCATGAT‑GG‑CGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAAcc < 1:638218/143‑1 (MQ=255) tGGAAGGCAGCGCCGCACCGCCATGAT‑GG‑CGCAGGATCAGGTTTTGCTCCGCCAGCCCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCCCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAAcc > 2:158570/1‑143 (MQ=255) aGGCAGCGCCGCACCGCCATGAT‑GG‑CGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGtt < 1:45119/143‑1 (MQ=255) cgccgcCCCGCCATGAT‑GG‑CGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGataa < 2:710854/143‑1 (MQ=255) cGCCATGAT‑GG‑CGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCCGTTCGATCATACCGTtg > 1:282110/1‑143 (MQ=255) | TCGAGCCATTGGGGATTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGAT‑GG‑C‑CAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTG > NC_000913/3560327‑3560585 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |