| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 123,050 | A→T | I12F (ATC→TTC) | aceE → | pyruvate dehydrogenase E1 component |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 123,050 | 0 | A | T | 97.3% | 106.0 / NA | 37 | I12F (ATC→TTC) | aceE | pyruvate dehydrogenase E1 component |
| Reads supporting (aligned to +/- strand): ref base A (0/0); major base T (16/20); minor base G (0/1); total (16/21) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
GCGCGGCAACTAAACGTAGAACCTGTCTTATTGAGCTTTCCGGCGAGAGTTCAATGGGACAGGTTCCAGAAAACTCAACGTTATTAGATAGATAAGGAATAACCCATGTCAGAACGTTTCCCAAATGACGTGGATCCGATCGAAACTCGCGACTGGCTCCAGGCGATCGAATCGGTCATCCGTGAAGAAGGTGTTGAGCGTGCTCAGTATCTGATCGACCAACTGCTTGCTGAAGCCCGCAAAGGCGGTGTAAACGTAGCCGCAGGCACAGGTATCA > NC_000913/122912‑123188 | gcgcGGCAACTAAACGTAGAACCTGTCTTATTGAGCTTTCCGGCGAGAGTTCAATGGGACAGGTTCCAGAAAACTCAACGTTATTAGATAGATAAGGAATAACCCATGTCAGAACGTTTCCCAAATGACGTGGATCCGTTCGa > 1:58612/1‑143 (MQ=255) ggCAACTAAACGTAGAACCTGTCTTATTGAGCTTTCCGGCGAGAGTTCAATGGGACAGGTTCCAGAAAACTCAACGTTATTAGATAGATAAGGAATAACCCATGTCAGAACGTTTCCCAAATGACGTGGATCCGTTCGAAACt > 1:156941/1‑143 (MQ=255) aCTAAACGTAGAACCTGTCTTATTGAGCTTTCCGGCGAGAGTTCAATGGGACAGGTTCCAGAAAACTCAACGTTATTAGATAGATAAGGAATAACCCATGTCAGAACGTTTCCCAAATGACGTGGATCCGTTCGAAACTcgcg > 1:404811/1‑143 (MQ=255) cTAAACGTAGAACCTGTCTTATTGAGCTTTCCGGCGAGAGTTCAATGGGACAGGTTCCAGAAAACTCAACGTTATTAGATAGATAAGGAATAACCCATGTCAGAACGTTTCCCAAATGACGTGGATCCGTTCGAAACTCGCGa < 2:407391/143‑1 (MQ=255) aCGTCGAACCTGGCTTATTGAGCTTTCCGGCGAGAGTTCAATGGGACAGGTTCCAGAAAACTCAACGTTATTAGATAGATAAGGAATAACCCATGTCAGAACGTTTCCCAAATGACGTGGATCCGTTCGAAACTCGCGACTgg < 1:11053/143‑1 (MQ=255) gctcaTTGAGCTTTCCGGCGAGAGTTCAATGGGACAGGTTCCAGAAAACTCAACGTTATTAGATAGATAAGGAATAACCCATGTCAGAACGTTTCCCAAATGACGTGGATCCGTTCGAAACTCGCGACTGGCTCCAGGCGATc < 2:110762/139‑1 (MQ=255) ttGAGCTTTCCCGCGAGAGTTCAATGGGACAGGTTCCAGAAAACTCAACGTTATTAGATAGATAAGGAATAACCCATGTCAGAACGTTTCCCAAATGACGTGGATCCGTTCGAAACTCGCGACTGGCTCCAGGCGATCGAATc < 1:424580/143‑1 (MQ=255) aGCTTTCCGGCGAGAGTTCAATGGGACAGGTTCCAGAAAACTCAACGTTATTAGATAGATAAGGAATAACCCATGTCAGAACGTTTCCCAAATGACGTGGATCCGTTCGCAACTCGCGACTCGCAACAGGCGATCGACTCgct > 2:95492/1‑141 (MQ=255) gCTTTCCGGCGAGAGTTCAATGGGACAGGTTCCAGAAAACTCAACGTTATTAGATAGATAAGGAATAACCCATGTCAGAACGTTTCCCAAATGACGTGGATCCGTTCGAAACTCGCGACTGGCTCCAGGCGATCGAATCGGTc < 2:551164/143‑1 (MQ=255) cGGCGAGAGTTCAATGGGACAGTTTCCAGAAAACTCAACGTTATTAGATAGATAAGGAATAACCCATGCCAGAACGTTTCCCAAATGACGTGGATCCGTTCGAAACTCGCGACTGGCTCCAGGCGATCGAATCGGTCATCCGt < 2:611657/143‑1 (MQ=255) ggCGAGAGTTCAATGGGACAGGTTCCAGAAAACTCAACGTTATTAGATAGATAAGGAATAACCCATGTCAGAACGTTTCCCAAATGACGTGGATCCGTTCGAAACTCGCGACTGGCTCCAGGCGATCGAATCGGTCATCCGTg > 1:168218/1‑143 (MQ=255) aaTGGGACAGGTTCCAGAAAACTCAACGTTATTAGATAGATAAGGAATAACCCATGTCAGAACGTTTCCCAAATGACGTGGATCCGTTCGAAACTCGCGACTGGCTCCAGGCGATCGAATCGGTCATCCGTGAAGAAGGTGtt < 1:344964/143‑1 (MQ=255) aaTGGGACAGGTGCAAGAAAACTCAACGTTATTAGATAGATAAGGAATAACCCATGTCAGAACGTTTCCCAAATGACGTGGATCCGTTCGAAACTCGCGACTGGCTCCAGGCGATCGAATCGGTCATCCGTGAAGAAGGTGtt < 1:357078/143‑1 (MQ=255) gggACAGGTTCCAGAAAACTCAACGTTATTAGATAGATAACGAATAACCCATGTCAGAACGTTTCCCAAATGACGTGGATCCGTTCGAAACTCGCGACTGGCTCCAGGCGATCGAATCGGTCATCCGTGAAGAAGGTGTTGAg < 1:511360/143‑1 (MQ=255) aGGTTCCAGAAAACTCAACGTTATTAGATAGATAAGGAATAACCCATGTCAGAACGTTTCCCAAATGACGTGGATCCGTTCGAAACTCGCGCCTGGCTCCCGGCGATCGACGCGGTCAGCCGTGAAGACGGCGTTGCGCGTGc > 2:687018/1‑143 (MQ=255) aGGTTCCAGAAAACTCAACGTTATTAGATAGATAAGGAATAACCCATGTCAGAACGTTTCCCAAATGACGTGGATCCGTTCGAAACTCGCGACTGGCTCCAGGCGATCGAATCGGTCATCCGTGAAGAAGGTGTTGAGCGTGc > 1:325215/1‑143 (MQ=255) cAGAAAACTCAACGTTATTAGATAGATAAGGAATAACCCATGTCAGAACGTTTCCCAAATGACGTGGATCCGTTCGAAACTCGCGACTGGCTCCAGGCGATCGAATCGGACATCCGAAAGGAAGGTGTTGAGCGTGCTCAGTa < 2:156941/143‑1 (MQ=255) aCTCAACATTATTAGATAGATAAGAGAGAACCCACGTCAGAACGGTGCCCAAATGACGTGGATCCGTTCGAAACTCGCGACTGGCTCCAGGCGATCGAATCGGTCATCCGTGAAGAAGGTGTTGAGCGTGCTCAGTATCTGAt < 1:319124/143‑1 (MQ=255) cTCAACGTTAGTAGATAGATAAGGAATAACCCATGTCAGAACGTTTCCCAAATGACGTGGATCCGTTCGAAACTCGCGACTGGCTCCAGGCGATCGAATCGGTCATCCGTGAAGAAGGTGTTGAGCGTGCTCAGTATCTGATc < 1:72887/143‑1 (MQ=255) cAACGTTATTGCATATATAAGGAATACCCCATGTCAGAACGTTTCCCAGATGGCGTGGATCCGTTCGAAACTCGCGACTGGCTCCAGGCGATCGAATCGGTCATCCGTGAAGAAGGTGTTGAGCGTGCTCAGTATCTGATCGa < 2:319133/143‑1 (MQ=255) aCGTTATTAGATAGATAAGGAATAACCCATGTCAGAACGTTTCCCAAATGACGTGGATCCGTTCGAAACTCGCGACTGGCTCCAGGCGATCGAATCGGTCATCCGTGAAGAAGGTGTTGAGCGTGCTCAGTATCTGATCGAcc < 1:35378/143‑1 (MQ=255) ttattaGATAGATAAGGAATAACCCATGTCAGAACGTTTCCCAAATGACGTGGATCCGTTCGAAACTCGCGACTGGCTCCAGGCGATCGAATCGGTCATCCGTGAAGAAGGTGTTGAGCGTGCTCAGTATCTGATCGACCAAc < 1:207554/143‑1 (MQ=255) ttatcaAATAGATAAGGAATAACCCATGTCAGAACGTTTCCCAAATGACGTGGATCCGTTCGAAACTCGCGACTGGCTCCAGGCGATCGAATCGGTCATCCGTGAAGAAGGTGTTGAGCGTGCTCAGTATCTGATCGACCAAc < 2:623301/143‑1 (MQ=255) ataggtgAGGAATAGCCCATGTCGGAACGTTTCCCAAATGACCTGCGTCCTGTCGAAACGCGCGACTGGCTCCAGGCGATCGACGCGGTCAGCCGTGAAGAAGGTGTTGAGCGTGCTCAGTATCTGATCGACCAACtgcttgc < 2:286716/143‑1 (MQ=255) taAGGAATAACCCATGTCAGAACGTTTCCCAAATGACGTGGATCCGTTCGAAACTCGCGACTGGCTCCAGGCGATCGAATCGGTCATCCGTGAAGAAGGTGTTGAGCGTGCTCAGTATCTGATCGACCAACTGCTTGCTGAAg < 1:309704/143‑1 (MQ=255) gAATAACCCATGTCAGAACGTTTCCCAAATGACGTGGATCCGTTCGAAACTCGCGACTGGCTCCAGGCGATCGAATCGGTCATCCGTGAAGAAGGTGTTGAGCGTGCTCAGTATCTGATCGACCAACTCCTTTCTGAACCCCg > 2:109244/1‑143 (MQ=255) tCAGAACGTTTCCCAAATGACGTGGATCCGTTCGAAACTCGCGACTGGCTCCAGGCGATCGAATCGGTCATCCGTGAAGAAGGTGTTGAGCGTGCTTAGTATCTGATCGACCAACTGCTTGCTGAAGCCCGCAAAGGCGgtgt > 2:424087/1‑143 (MQ=255) cAGAACGTTTCCCAAATGACGTGGATCCGTTCGAAACTCGCGACTGGCTCCAGGCGACCGAATCGGTCATACGTAAAGAAAGTATTGAACGTGCTCAGTATCTGATCGACCAACTGCTTGCTGAAGCCCGCAAAGGCGGTGTa > 1:206908/1‑143 (MQ=255) aGAACGTTTCCCAAATGACGTGGATCCGTTCGAACCTCGCGCCTGGCCCCACGCGATCGAATCGGTCATCCGTGAAGAAGGTGTCGAGCGTGCCCAGTATCCGATCGGCCACCTGCCTGCTCACCCCCCCAAAGGcagcgtcc > 2:700657/1‑136 (MQ=255) gAACGTTTCCCAAATGACGTGGATCCGTTCGAAACTCGCGACTGGCTCCAGGCGATCGAATCGGTCATCCGTGAAGAAGGTGTTGAGCGTGCTCAGTATCTGATCGACCAACTGCTTGCTGAAGCCCGCAAAGGCGGTGTaaa < 1:383615/143‑1 (MQ=255) aaCGTTTCCCAAATGACGTGGATCCGTTCGAAACTCGCGACTGGCTCCAGGCGATCGAATCGGTCATCCGTGAAGAAGGTGTTGAGCGTGCTCAGTATCTGATCGACCAACGGCTTGCTGAAGCCCGCAAAGGCGGTGTAAAc > 1:70236/1‑143 (MQ=255) ccAAATGACGTGGATCCGTTCGAAGCTCGCGACTGGCTCCAGGCGATCGAATCGGTCATCCGTGAAGAAGGTGTTGAGCGTGCTCAGTATCTGATCGACCAACTGCTTGCTGAAGCCCGCAAAGGCGGTGTAAACGTAGCCGc < 2:447978/143‑1 (MQ=255) ccAAATGACGTGGATCCGTTCGAAACTCGCGACTGGCTCCAGGCGATCGAATCGGTCATCCGTGAAGAAGGTGTTGAGCGTGCTCAGTATCTGATCGACCAACTGCTTGCTGAAGCCCGCAAAGGCGGTGTAAACGTAGCCGc > 1:19331/1‑143 (MQ=255) cGTGGCTTCGTTCGAAGTGCGCGGCTGGCTCCAGTGGATCGAATCGGTCATCCGTGAAGAAGGTGTTGAGCGTGCTTAGTATCTGATCGACCAACTGCTTGCTGAAGCCCGCAAAGGCGGTGTAAACGTAGCCGCAGGCACAg < 2:608797/143‑1 (MQ=255) gTGGATCCGTTCGAAACTCGCGACTGGCTCCAGGCGATCGAATCGGTCATCCGTGAAGAAGGTGTTGAGCGTGCTCAGTATCTGATCGACCAACTGCTTGCTGAAGCCCGCAGAGGCGGTGTAGACGTAGCCGCGGGCACAgg > 1:76716/1‑143 (MQ=255) gTGGATCCGTTCGAAACTCGCGACTGGCTCCAGGCGATCGAATCGGTCATCCGTGAAGAAGGTGTTGAGCGTGCTCAGTATCTGATCGACCAACTGCTTGCTGAAGCCCGCAAAGGCGGTGTAAACGTAGCCGCAGGCACAgg > 1:337595/1‑143 (MQ=255) tCCGTTCGAAACTCGCGACTGGCTCCAGGCGATCGAATCGGTCATCCGTGAAGAAGGTGTTGAGCGTGCTCAGTATCTGATCGACCAACTGCTTGCTGAAGCCCGCAAAGGCGGTGTAAACGTAGCCGCAGGCACAGGTATCa > 1:33282/1‑143 (MQ=255) | GCGCGGCAACTAAACGTAGAACCTGTCTTATTGAGCTTTCCGGCGAGAGTTCAATGGGACAGGTTCCAGAAAACTCAACGTTATTAGATAGATAAGGAATAACCCATGTCAGAACGTTTCCCAAATGACGTGGATCCGATCGAAACTCGCGACTGGCTCCAGGCGATCGAATCGGTCATCCGTGAAGAAGGTGTTGAGCGTGCTCAGTATCTGATCGACCAACTGCTTGCTGAAGCCCGCAAAGGCGGTGTAAACGTAGCCGCAGGCACAGGTATCA > NC_000913/122912‑123188 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |