Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,917,603	T→G	V849V (GTT→GTG)	barA →	sensory histidine kinase BarA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,917,603	0	T	G	100.0%	83.0 / NA	33	V849V (GTT→GTG)	barA	sensory histidine kinase BarA
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base G (16/17);  total (16/17)

GGTGAACCCGAATGCGACCCTCGACTGGCAACTGGCACTACGCCAGGCAGCAGGAAAAACCGATTTAGCGCGCGATATGCTGCAAATGTTACTCGATTTCCTGCCTGAAGTTCGCAACAAAGTTGAGGAACAGCTGGTTGGAGAAAACCCGGAAGGCCTGGTTGATTTGATTCATAAACTGCATGGCAGTTGCGGCTATAGCGGTGTGCCACGTATGAAGAATCTCTGCCAACTGATCGAACAACAGCTACGTAGCGGTACTAAAGAAGAAG  >  NC_000913/2917465‑2917736                                                                                                                                           |                                                                                                                                      ggtgAACCTGAATGCGAGCCTCCACTGGTAACTGGCACTACGCCAGGCAGCAGGAAAGACCGATTTAGCGCGCGATATGCTGCAAATGTTACTCGATTTCCTGCCTGAAGTTCGCAACAAAGTTGAGGAACAGCTGGTGGgag                                                                                                                                   <  2:73913/143‑1 (MQ=255) ggtgAACCCGAATGCGACCCTCGACTGGCAACTGGCACTACGCCAGGCAGCAGGAAAAACCGATTTAGCGCGCGATATGCTGCAAATGTTACTCGATTTCCTGCCTGAAGTTCGCAACAAAGTTGAGGAACAGCTGGTGGgag                                                                                                                                   >  2:663360/1‑143 (MQ=255)    gAACCCGAATGCGACCCTCGACTGGCAACTGGCACGACGCCAGGCAGCAGGCAAAACCGATTTAGCGCGCGACATGCGGCAACTGTTACTCGATTTCCTGCCTGGCGTTCGCAACCAAGTTGAGGAACAGCAGGGGGgaggac                                                                                                                                >  2:660625/1‑140 (MQ=255)        ccGAATGCGTCTCTCGCCTGGCAACTGGCACTACGCCAGGCAGCAGGAAAAACCGATTTAGCGCGCGATATGCGGTAAATGTTACTCGATTTCCTGCCTGAAGTTCGCAACAAAGTTGAGGAACAGCTGGTGGGAGAAAAccc                                                                                                                            <  2:333085/143‑1 (MQ=255)         cGAATGCGACCCTCGACTGGCAACTGGCACTACGCCAGGCAGCAGGAAAAACCGATTTAGCGCGCGATATGCTGCAAATGTTACTCGATTTCCTGCCTGAAGTTCGCAACAAAGTTGAGGAACAGCTGGTGGGAGAAAACCCg                                                                                                                           <  1:302179/143‑1 (MQ=255)                            gcaactggcaCTACGCCAGGCAGCAGGAAAAACCGATTTAGCGCGCGATATGCTGCAAATGTTACTCGATTTCCTGCGTGAAGATCGCAACAAAGTTGAGGAACAGCTGGTGGGAGAAAACCCGGAAGGCCTGGttgagctga                                                                                                        >  2:297683/1‑138 (MQ=255)                                     aCTACGCCAGGCAGCAGGAAAAACCGATTTAGCGCGCGATATGCTGCAAATGTTACTCGATTTCCTGCCTGAAGTTCGCAACAAAGTTGAGGAACAGCTGGTGGGAGAAAACCCGGAAGGCCTGGTTGATTTGATTCATAAAc                                                                                               >  2:692183/1‑143 (MQ=255)                                         ccacaggcaggAGGAAAAACCGATTTATCGCGTGATGTGCGGCAAATGTGGCGCGATTTGCCGCCTGAAGTCCCCAACAAAGTTGAGGAACAGCTGGTGGTAGAAAACCCGGAAGGCCTGGTTGATTTGATTACTAAACTGCa                                                                                           <  2:679237/140‑1 (MQ=255)                                                cagcagGAAAAACCGATTTAGCGCGCGATATGCTGCAAATGTTACTCGATTTCCTGCCTGAAGTTCGCAACAAAGTTGAGGAACAGCTGGTGGGAGAAAACCCGGAAGGCCTGGTTGATTGCATTGACAAACTGCATGGCAGt                                                                                    >  2:343568/1‑143 (MQ=255)                                                           aCCGATTTAGCGCGCGATATGCTGCAAATGTTACTCGATTTCCTGCCTGAAGTTCGCAACAAAGTTGAGGAACAGCTGGTGGGAGAAAACCCGGAAGGCCTGGTTGATTTGATTCATAAACTGCATAGCAGATGCGGCGATAg                                                                         >  2:369857/1‑143 (MQ=255)                                                            ccGATTTAGCGCGCGATATGCTGCAAATGTTACTCGATTTCCTGCCTGAAGTTCGCAACAAAGTTGAGGAACAGCTGGTGGGAGAAAACCCGGAAGGCCTGGGTGATTTGATTCATAAACTGCATGGCAGTTGCGGCTATAGc                                                                        >  1:684783/1‑143 (MQ=255)                                                               ttttAGCGCGCGAGATGCTGCAAATGTTACTCGATTTCCTGCCTGAAGTTCGCAACAAAGTTGAGGAACAGCTGGTGGGAGAAAACCCGGAAGGCCTGGTTGATTTGATTCATAAACTGCATGGCAGTTGCGGCTATAGCGgt                                                                     <  2:162566/142‑1 (MQ=255)                                                                tttAGCGCGCGATATGCTGCAAATGTTACTCGATTTCCTGCCTGAAGTTCGCAACAAAGTTGAGGAACAGCTGGCGGGCGAAGACCCGGAGGGCCTGGTTGATTTGATTCATAAACTGCATGGCAGTTGCGGCTATAGCGgtg                                                                    >  1:165519/1‑143 (MQ=255)                                                                 ttAGCGCGCGATATGCTGCAAATGTTACTCGATTTCCTGCCTGAAGTTCGCAACAAAGTTGAGGAACAGCTGGTGGGAGAAAACCCGGAAGGCCTGGTTGATTTGATTCATAAACTGCATGGCAGTTGCGGCTATAGCGgtgt                                                                   >  1:123826/1‑143 (MQ=255)                                                                         cgATATGCTGCAAATGTTACTCGATTTCCTGCCTGAAGTTCGCAACAAAGTTGAGGAACAGCTGGTGGGAGAAAACCCGGAAGGCCTGGTTGATTTGATTCATAAACTGCATGGCAGTTGCGGCTATAGCGGTGTGCCACGTa                                                           <  1:144786/143‑1 (MQ=255)                                                                         cgATATGCTGCAAATGTGACGCGATCGCCTGCCGGAAGTTCGCAACAAAGTGGAGGAACAGCTGGTGGGAGAAAACCCGGAAGGCCTGGTTGATTTGATTCATAAACTGCATGGCAGTTGCGGCTATAGCGGTGTGCCACGTa                                                           <  1:696578/143‑1 (MQ=255)                                                                          gATATGCTGCAAATGTTACTCGATTTCCTGCCTGAAGTTCGCAACAAAGTTGAGGAACAGCTGGTGGGAGAAAACCCGGAAGGCCTGGTTGATTTGATTCATCAGCTGCATGGCAGTTGCGGCTATAGCGGTGTGCCACGTAt                                                          <  2:635457/143‑1 (MQ=255)                                                                            tatGCTGCGAATGTTACTGGATTCCCGGCCGGAAGTTCGCAACAAAGTTGAGGAGCAGCTGGTGGGAGAAAACCCGGAAGGCCTGGTTGATTTGATTCATAAACTGCATGGCAGTTGCGGCTATAGCGGTGTGCCACGTATga                                                        <  2:24150/143‑1 (MQ=255)                                                                                     attGTTACTCGATTTCCTGCCTGGAGGTCGCAACAAAGTTGAGGAACAGCTGGTGGGAGAAAACCCGGAAGGCCTGGTTGATTTGATTCATAAACTGCATGGCAGTTGCGGCTATAGCGGTGTGCCACGTATGAAGAATctct                                               <  2:668080/141‑1 (MQ=255)                                                                                      aTGTTACTCGATTTCCTGCCTGAAGTTCGCAACAAAGTTGAGGAACAGCTGGTGGGAGAAAACCCGGAAGGCCTGGTTGATTTGATTCATAAACTGCATGGCAGTTGCGGCTATAGCGGTGTGCCACGTATGAAGGATCTCTg                                              >  1:448658/1‑143 (MQ=255)                                                                                           aCTCGATTTCCTGCCTGAAGTTCGCAACAAAGTTGAGGAACAGCTGGTGGGAGAAAACCAGGAAGGCCTGGTTGATTTGATTCATAAACTGCATGGCAGTTGCGGCTATAGCGGTGTGCCACGTATGAAGAATCTCTGCCAAc                                         >  2:640956/1‑143 (MQ=255)                                                                                            cTCGATTTCCTGCCTGAAGTTCGCAACAAAGTTGAGGAACAGCTGGTGGGAGAAAACCCGGAAGGCCTGGTTGATTTGATTCATAAACTGCATGGCAGTTGCGGCTATAGCGGTGTGCCACGTATGAAGAATCTCTGCCAACt                                        >  2:686592/1‑143 (MQ=255)                                                                                             tCGATTTCCTGCCTGAAGTTCGCAACAAAGTTGAGGGACAGCTGGTGGGAGAAAACCCGGAAGGCCTGGTTGATTTGATTCATAAACTGCATGGCAGTTGCGGCTATAGCGGTGTGCCACGTATGAAGAATCTCTGCCAACTg                                       >  1:470269/1‑143 (MQ=255)                                                                                                   tCCTGCCGGAAGGTCGCAACAAAGTTGAGGAACAGCTGGTGGGAGAAAACCCGGAAGGCCTGGTTGATTTGATTCATAAACTGCATGGCAGTTGCGGCTATAGCGGTGTGCCACGTATGAAGAATCTCTGCCAACTGATCGaa                                 <  2:712051/143‑1 (MQ=255)                                                                                                                 cGCAACAAAGTTGAGGAACAGCTGGTGGGAGAAAACCCGGAAGGCCTGGTTGATTTGATTCATAAACTGCATGGCAGTTGCGGCTATAGCGGTGTGCCACGTATGAAGAATCTCTGCCAACTGATCGAACAACAGCTACGTAg                   <  2:372725/143‑1 (MQ=255)                                                                                                                     acaaAGCTGAGGAACAGCTGGTGGGAGCAAGCCCGGAAGGCCTGGTTGATTTGATCCATAAACTGCATGGCAGCTGCGGCTATAGCGGTGTGCCACGTATGAAGAATCTCTGCCAACTGATCGAACAACAGCTACGTAGCGGt               <  2:437910/143‑1 (MQ=255)                                                                                                                       aaaGTTTAGGAACAGCTGGTGGGAGAAAACCCGGAATGCCTGGTTGATTTGATTCATAAACTGCATGGCAGTTGCGGCTATAGCGGTGTGCCACGTATGAAGAATCTCTGCCAACTGATCGAACAACAGCTACGTAGCGGTAc             <  1:621504/143‑1 (MQ=255)                                                                                                                          gTTGAGGAACAGCTGGTGGGAGAAAACCCGGAAGGCCTGGTTGATTTGATTCATAAACTGCATGGCAGTTGCGGCTATAGCGGTGTGCCACGTATGAAGAATCTCTGCCAACTGATCGAACAACAGCTACGTAGCGGTACTaa          <  2:140322/143‑1 (MQ=255)                                                                                                                           ttGAGGAACAGCTGGGGGGAGAAAACCCGGGAGGCCTGGTTGATTTGATTCATAAACTGCATGGCAGTTGCGGCTATAGCGGTGTGCCACGTATGAAGAATCTCTGCCAACTGATCGAACAACAGCTACGTAGCGGTACTaaa         <  1:392361/143‑1 (MQ=255)                                                                                                                                gAACAGCTGGTGGGAGAAAACCCGGAAGGCCTGGTTGATTTGATTCATAAACTGCATGGCAGTTGCGGCTATAGCGGTGTGCCACGTATGAAGAATCTCTGCCAACTGATCGAACAACAGCTACGTAGCGGTACTAcagcaga    >  2:353449/1‑143 (MQ=255)                                                                                                                                 aaCAGCTGGCGGGAGAAAACCCGGAAGGCCTGGTTGATTTGATTCATAAACTGCATGGCAGTTGCGGCTATAGCGGTGTGCCACGTATGAAGAATCTCTGCCAACTGATCGAACAACAGCTACGTAGCGGTACTAaagaagaa   <  1:517102/143‑1 (MQ=255)                                                                                                                                  aCAGCTGGTGGGAGAAAACCCGGAAGGCCTGGTTGATTTGATTCATAAACTGCATGGTAGTTGCGGCTATAGCGGTGTGCCACGTATGAAGAATCTCTGCCAACTGATAGAACAACAGCTACGTAGAGGTACTAaagaagaag  >  1:232121/1‑143 (MQ=255)                                                                                                                                  aCAGCTGGTGGGAGAAAACCCGGAAGGCCTGGTTGATTTGATTCATAAACTGCATGGCAGTTGCGGCTATAGCGGTGTGCCACGTATGAAGAATCTCGGCCAACTGATCGAACAACAGCTACGTAGCGGTACGAaagaagaag  >  2:187647/1‑143 (MQ=255)                                                                                                                                           |                                                                                                                                      GGTGAACCCGAATGCGACCCTCGACTGGCAACTGGCACTACGCCAGGCAGCAGGAAAAACCGATTTAGCGCGCGATATGCTGCAAATGTTACTCGATTTCCTGCCTGAAGTTCGCAACAAAGTTGAGGAACAGCTGGTTGGAGAAAACCCGGAAGGCCTGGTTGATTTGATTCATAAACTGCATGGCAGTTGCGGCTATAGCGGTGTGCCACGTATGAAGAATCTCTGCCAACTGATCGAACAACAGCTACGTAGCGGTACTAAAGAAGAAG  >  NC_000913/2917465‑2917736

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑